The present study was inspired by scientific reports describing the positive effects of silicon preparations on fruit and vegetables in horticulture. The use of silicon preparations in forest protection represents a new research application, especially in the cases of oak seedlings that are damaged by oak powdery mildew. Additionally, these preparations increase the photosynthetic efficiency of the seedlings, and thus promote increased biomass and better nutritional value of the root systems. The new idea of using silicon formulations in forestry, based on the initial test results, is particularly important for seedlings in the post-planting period in forest plantations. In particular, these preparations increase yield and plant growth, and improve plant health. So far, no comparable studies have been conducted on forest tree species. To this end, one year-old pine (Pinus sylvestris L.) and two year-old English oak (Quercus robur L.) seedlings were sprayed with silicon preparations of different concentrations, and changes in growth parameters and photosynthetic efficiency were observed. The main objective of the study was to determine the effect of the silicon preparation on the seedlings during their first life span, i.e., the first year after planting. In this study, 50 pine and 50 oak seedlings were sprayed three times with the silicon preparation, in five variants (control; concentrations of 1%, 2%, 3% and 5%), as well as the chlorophyll fluorescence, height, thickness and biomass of the roots, were measured. It was found that the treatment with a concentration of 2% was better and statistically, significantly different from the other variants, e.g., in terms of increasing photosynthetic efficiency.

Dispersal mechanisms of phytopathogenic fungi and oomycetes are critical components of forest disease dynamics. While wind and water are well-studied pathways, the role of large forest herbivores as passive vectors remains significantly overlooked. This study quantifies and compares the pathogen loads carried on the hooves and hair of wild red deer (Cervus elaphus) to evaluate their epidemiological potential. Swab samples were collected from the hooves and hair of harvested deer in the Czerwony Bór Forest District, Poland. Quantitative PCR (qPCR) assays targeting the ITS1 region were deployed to detect total fungal DNA, Alternaria alternata, Fusarium avenaceum/F. tricinctum, and several Phytophthora species. A linear mixed-effects model was implemented to statistically evaluate variations in pathogen loads across anatomical sampling locations while controlling for individual animal variability. Fungal DNA was detected in 87.5% of hoof samples, showing significantly lower Ct values (13.85–18.54) compared to fur samples (17.02–29.56), which exhibited a more patchy distribution (p = 0.016). Similarly, A. alternata transfer was highly favored by hooves (p < 0.001). Conversely, F. avenaceum was more frequently detected on hair. Among oomycetes, Phytophthora pseudosyringae was detected in all sampled animals, whereas Phytophthora cactorum occurred rarely, and other tested Phytophthora species were not detected. Wild deer carry DNA of multiple fungal and oomycete pathogens and may act as potential passive carriers within forest ecosystems. Hooves constitute the primary vector for soil-borne pathogens due to sustained contact with topsoil, whereas hair facilitates the movement of specific canopy or airborne taxa. These findings suggest that wildlife movements should be considered in future forest biosecurity assessments for comprehensive forest health management and for understanding pathogen exchange between forest and agricultural ecosystems.