Miscanthus x giganteus (miscanthus), a perennial biomass crop, allocates more carbon belowground and typically has lower soil greenhouse gas (GHG) emissions than conventional feedstock crops, but best practices for nutrient management that maximize yield while minimizing soil GHG emissions are still debated. This study evaluated the effects of four different fertilization treatments (digestate from a biodigester, synthetic fertilizer (urea), hydrochar from the hydrothermal carbonization of digestate, and a control) on soil GHG emissions and biomass yield of an established miscanthus stand grown on abandoned agricultural land. Soil GHG fluxes (including CH₄, CO₂, and N₂O) were sampled in all treatments using the static chamber methodology. Average biomass yield varied from 20.2 Mg ha⁻¹ to 23.5 Mg ha⁻¹, but there were no significant differences among the four treatments (p > 0.05). The hydrochar treatment reduced mean CO₂ emissions by 34% compared to the control treatment, but this difference was only statistically significant in one of the two sites tested. Applying digestate to miscanthus resulted in a CH₄ efflux from the soil in one of two sites, while soils treated with urea and hydrochar acted as CH₄ sinks in both sites. Overall, fertilization did not significantly improve biomass yield, but hydrochar as a soil amendment has potential for reducing soil GHG fluxes.

Terrestrial consumption of the potent greenhouse gas methane (CH₄) is a critical aspect of the future climate, as CH₄ concentrations in the atmosphere are projected to play an increasingly important role in global climate forcing. Anaerobic oxidation of methane (AOM) has only recently been considered a relevant control on methane fluxes from terrestrial systems. We performed in vitro anoxic incubations of intact peat from Utqiaġvik (Barrow), Alaska using stable isotope tracers. Our results showed an average potential AOM rate of 15.0 nmol cm³ h⁻¹, surpassing the average rate of gross CH₄ production (6.0 nmol cm³ h⁻¹). AOM and CH₄ production rates were positively correlated. While CH₄ production was insensitive to additions of Fe(III), there was a depth:Fe(III) interaction in the kinetic reaction rate constant for AOM, suggestive of stimulation by Fe(III), particularly in shallow soils (<10 cm). We estimate AOM would consume 25–34% of CH₄ produced under ambient conditions. Soil genetic surveys showed phylogenetic links between soil microbes and known anaerobic methanotrophs in ANME groups 2 and 3. These results suggest a prevalent role of AOM to net CH₄ fluxes from Arctic peatland ecosystems, and a probable link with Fe(III)-reduction.

Small-scale anaerobic digestion (AD) can be an effective organic waste management system that also provides energy for small businesses and rural communities. This study measured fuel production from digestions of single and mixed feedstocks using an unheated, 2 m³ digester operated continuously in a temperate climate for over three years. Using local food waste, brewery waste, grease waste, and agricultural residues, this study determined that small-scale AD co-digestions were almost always higher yielding than single feedstocks during psychrophilic operation and seasonal temperature transitions. Agricultural residues from Miscanthus x giganteus had the greatest impact on biomethane production during co-digestion (4.7-fold greater average biogas %CH₄), while mesophilic digestion of brewery waste alone produced the most biogas (0.76 gCH₄ gVS⁻¹ d⁻¹). Biogas production during the transition from mesophilic to psychrophilic was temporarily maintained at levels similar to mesophilic digestions, particularly during co-digestions, but biogas quality declined during these temperature shifts. Full-time operation of small-scale, unheated AD systems could be feasible in temperate climates if feedstock is intentionally amended to stabilize carbon content.

Food waste (FW), a major part of the US waste stream, causes greenhouse gases within landfills, but there is an opportunity to divert FW to anaerobic digestion (AD) facilities that produce biogas and digestate fertilizer. The composition of FW inputs to AD determines the value of these products. This study provides insight into the effect of waste composition on the quality of AD products by first characterizing the biogas and digestate quality of anaerobically digested FW from four diets (paleolithic, ketogenic, vegetarian, and omnivorous), and then estimating the difference in biogas produced from codigested FW and brewery waste (BW). Waste feedstock mixtures were incubated in lab-scale bioreactors for 21 days with live inoculum. Biogas quality was monitored for 21–30 days in four trials. Samples were analyzed using a gas chromatograph for detection of methane (CH₄) and carbon dioxide (CO₂). The composition of the waste inputs had a significant impact on the quality of biogas but not on the quality of the digestate, which has implications for the value of post-AD fertilizer products. Wastes with higher proportions of proteins and fats enhanced biogas quality, unlike wastes that were rich in soluble carbohydrates. Codigestion of omnivorous food waste with carbon-rich agricultural wastes (AW) improved biogas quality, but biogas produced from BW does not necessarily improve with increasing amounts of AW in codigestion.