The aim of this study was to determine dynamic thiol-disulphide homeostasis (TDH) parameters and ischemia modified albumin (IMA) levels in calves with neonatal diarrhea and compare with healthy controls. A total of 50 calves were included in the study. There were 25 calves in both diarrhea and healthy groups. Serum native thiol, total thiol, disulfide and IMA amounts were measured using new methods. Native thiol (p=0.025) and total thiol (p=0.041) values decreased significantly in calves with neonatal diarrhea compared to the healthy control group. Disulphide (p= 0.133), % disulphide/native thiol ratio (p=0.001) and IMA (p=0.0018) parameters were lower in healthy group, and the difference between the two groups was significant for the parameters other than disulphide. This study shows that TDH is impaired in neonatal calf diarrhea and IMA levels are increased due to oxidative stress.

Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii, which occurs worldwide in mammals and birds. Brain is the primary target organ because Toxoplasma gondii is a ubiquitous intracellular parasite that causes most frequently life-threatening encephalitis in immunocompromised patients. Relation of tissue cysts number, histopathology score and acute phase proteins were investigated. In this study, 36 mice are infected with Me49 strain of Toxoplasma gondii. The control group has 6 healthy mice. After inoculation of Toxoplasma gondii, at 10., 15., 20., 30., 45., 60. days, 6 each mice euthanized after collection of blood samples. Hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin levels are determined by ELISA. Then, brain tissues were investigated histopathologically and lesions were scored. The average cyst numbers were determined by counting three samples (25 μl each) of each brain homogenate under light microscopy. Inflammatory reaction was observed on day 10 days after inoculation (d.a.i.) The lesions were characterized by perivascular mononuclear cell infiltration, focal mononuclear cell infiltration in the meninges, and glial proliferation. Tissue cysts were observed in all Toxoplasma gondii-infected groups. The highest lesion score was observed at 60 d.a.i. And the most tissue cyst number were on day 30. d.a.i. Serum levels of hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin were significantly higher than the control group on day 10–20., 10., 10–30., 10.,10–45 d.a.i., respectively. High level of acute phase proteins in mice on certain days infected with Toxoplasma gondii was exhibited a relationship between brain lesions and tissue cysts.

The objective of this study was to evaluate cardiac remodeling and the diagnostic utility of cardiac biomarkers, cardiac troponin I (cTnI) and N-terminal pro–B-type natriuretic peptide (NT-proBNP), in cats with chronic kidney disease (CKD), with or without systemic hypertension. The study population included 11 healthy controls, 11 normotensive cats with chronic kidney disease (CKD), and 16 hypertensive cats with CKD. All cats underwent a comprehensive evaluation including blood pressure measurement, serum biochemistry, biomarker analysis, and echocardiographic assessment. CKD cats exhibited significantly elevated serum concentrations of cTnI and NT-proBNP compared to the control group, with hypertensive cats demonstrating the highest levels of these markers (P

Pulmonary veno-occlusive disease (PVOD) is an uncommon disease in humans and animals caused by pulmonary hypertension. The aim of this study was to investigate pathomorphological findings of PVOD in a cat. A mass was detected in the right atrium with echocardiography in an 11-year-old male cat. Following treatments, the cat was presented for necropsy after death due to respiratory failure. Grossly, masses were detected in mesenteric lymph node, mediastinal lymph node and, heart. At lungs examination, consolidated areas with edema and congestion were seen. Histopathologically, neoplastic lymphoid cells were observed in intestine, lymph nodes and heart. In lungs, vein lumens were almost completely occluded by mostly loose intimal fibrosis. Near affected veins, increasing in number of the capillaries was recognized. While intimal fibrosis was detected by Masson’s trichrome staining, the occluded veins were demonstrated by Verhoeff-van Gieson staining. PVOD and intestinal lymphoma with cardiac metastasis were diagnosed in a male cat after clinico-pathological examinations. With this case, pathomorphological evaluation of PVOD was reported in a cat and the relationship with PVOD and lymphoma was discussed. As a result of clinico-pathological examinations; PVOH, intestinal lymphoma, and cardiac metastasis were described in a male cat. Pulmoner Veno-Oklüzif Hastalık (PVOH) pulmoner hipertansiyonun neden olduğu, insanlarda ve hayvanlarda nadir görülen bir hastalıktır. Bu olgu ile bir kedide gözlenen Pulmoner Veno-Oklüzif Hastalık’ın patomorfolojik yönden incelenmesi amaçlanmıştır. 11 yaşlı erkek kedinin ekokardiyografisinde sağ atriumda kitle gözlendi. Tedavilerin ardından solunum güçlüğüne bağlı ölen kedinin nekropsisi yapıldı. Makroskobik olarak, mezenteriyal ve mediastinal lenf yumrularında ve kalpte kitleler ile karşılaşıldı. Akciğer muayenesinde ödem ve konjesyon ile konsolide alanlar görüldü. Histopatolojik olarak bağırsak, lenf yumruları ve kalpte neoplastik lenfoid hücreler gözlendi. Akciğerde şiddetli intimal fibrozise bağlı venlerin lümenlerinde daralma ve tıkanmalar dikkati çekti. Etkilenen çoğu damarların çevresinde kapillarizasyon gözlendi. Masson trikrom boyamasıyla intimal fibrozis tespit edilirken, tıkalı damarlar Verhoeff-van Gieson boyamasıyla gösterildi. Yapılan klinikopatolojik incelemeler sonrasında PVOD ve kardiyak metastazlı intestinal lenfoma tanıları konuldu. Bu olguda bir kedide Pulmoner Veno-Oklüzif Hastalık patomorfolojik yönden değerlendirilmiş ve PVOD ile lenfoma ile ilişkisi tartışılmıştır. Klinikopatolojik incelemeler sonucunda erkek bir kedide PVOH, intestinal lenfoma ve kardiyak metastazı tanımlanmıştır.

Two-dimensional speckle tracking echocardiography (2D-STE) is a new approach developed for cardiac imaging that provides better assessment of regional and global myocardial abnormalities than standard echocardiography techniques. Latest studies have demonstrated that asymptomatic and mildly symptomatic patients with hypertrophic cardiomyopathy have variable areas of patchy myocardial fibrosis in left ventricular myocardium. However, no previous studies in cats with feline arterial thromboembolism (ATE) have used 2D-STE to assess myocardial function. The purpose of the study was to evaluate regional radial strain variables of the left ventricle using 2D-STE in cats with ATE. Ten cats affected with ATE and ten healthy control cats were studied. Cats with ATE, in the study group, were diagnosed with hypertrophic cardiomyopathy (HCM). This group was further divided into both intraventricular septum (IVS) and left vetricular (LV) hypertrophy (IVS-HCM, n:5) and only LV free wall hypertrophy (LV-HCM, n:5) groups. Compared to the control group, cats in LV-HCM and IVS-HCM groups had a thicker IVSd. LVFWd were considerably higher in LV-HCM group than in both IVS-HCM and the control group (8.04 ± 0.93, 4.9 ± 0.4, 3,91 ± 0,17 respectively, P

Kedi ve köpeklerde kalp hastalıklarının doğru tanısı, yalnızca klinik muayeneye dayanmakla sınırlı kalmamalı; çeşitli tanı yöntemlerinin