This study investigated the presence of Mycoplasma bovis in nasal swabs taken from cattle with pneumonia, to reveal the clinical and biochemical findings of positive animals, and determine the levels of vitamin A and 25(OH)D₃. In the study were included 103 infected cattle and 10 healthy cattle, all aged 1-4 years, as the control group. Quantitative clinical evaluations and clinical examinations were performed on each animal, and 5 ml blood samples and nasal swabs were taken. Serum biochemistry, vitamin A and 25(OH)D₃ levels were determined in both positive and control samples. The clinical signs were identified by qualitative scoring in M. bovis positive animals. Regarding the clinical findings, respiratory frequency, heart rate, and rectal temperature were significantly higher in positive than control group animals (p<0.001). Regarding the biochemistry findings, albumin (ALB) and glucose (GLU) levels were lower in cattle infected with M. bovis (p<0.001) whereas total protein (TP) and alkaline phosphatase (ALP) levels were higher (p<0.001). While serum 25(OH)D₃ levels were lower in infected animals (p0.05) in serum vitamin A levels. In this study, clinical findings in cattle naturally infected with M. bovis were expressed qualitatively and quantitatively. In addition, it has been revealed that the disease does not only cause lung involvement, but also causes changes in biochemical and vitamin levels. Although these parameters cannot be used as a biomarker in the diagnosis of pneumonia, they are thought to provide benefits in terms of diagnosis, treatment and prophylaxis in practice.

Aggression is a prevalent and concerning behavioral issue in dogs. Pit Bull dogs, known for their high levels of aggression, are recognized as a focus of concern in society. In our study, we aimed to investigate the behavioral characteristics of Pit Bull dogs and explore the potential roles of peptides involved in the neurobiology of aggression. Initially, female, and male dogs underwent aggression tests, and their aggression levels were categorized. Plasma nesfatin-1, serotonin, oxytocin, and dopamine levels were quantified using ELISA, with blood samples collected after a 24 h fasting period and 2 h post-refeeding. Our findings indicate that aggression in Pit Bull dogs correlates with decreased plasma nesfatin-1, serotonin, and oxytocin levels, while dopamine levels increase. The study’s findings indicate that fasted dogs exhibited lower plasma levels of nesfatin-1, serotonin, and dopamine, while plasma oxytocin levels were higher. Furthermore, while the research findings do not suggest a significant relationship between the severity of aggression and the gender of the dog, male Pit Bull breeds appear to have higher plasma nesfatin-1 and serotonin levels compared to their female counterparts. The study’s findings demonstrate that nesfatin-1, serotonin, oxytocin, and dopamine play pivotal roles in Pit Bull dogs’ aggression, indicating potential interactions among these neuropeptides at the central nervous system level.

The present study was conducted to investigate the effect of eutocia and dystocia on blood gas and Apgar scores in Aleppo goat kids. A total of 40 kids were used in the study. Kids were divided into 2 groups according to the type of birth. The first group of the study (Group 1, n=20) consisted of kids born as a result of eutocia, and the second group of the study (Group 2, n=20) was formed as a result of dystocia. Apgar scores (appearance, pulse, grimace, attitude, respiration) were evaluated at 0, 5, and 15 minutes postpartum from kids in both study groups, and blood samples were taken from blood gas injectors and blood gas profiles (pH, pCO2, pO2, HCO3) were obtained. The obtained data were analyzed with Mann-Whitney-U and Friedman post-hoc bonferroni. Spearman correlation coefficients were calculated to determine the relationships between measurements. A statistically significant difference was observed between the groups in the Apgar score at 0, 5 and 15 minutes and in the blood gas measurements of pH, pO2, pCO2, and HCO3 at 0, 5 and 15 minutes (p

Ovine pneumonia is a multifactorial respiratory disease, yet bacterial community alterations associated with pathologically confirmed pneumonia in lambs remain poorly characterised. This study investigated the lung bacterial microbiota of non-pneumonic and pneumonic Akkaraman lambs by integrating gross and histopathological examination with 16S rRNA gene amplicon sequencing. The final dataset comprised lung samples from 15 non-pneumonic and 14 pneumonic lambs. Alpha diversity did not differ significantly between groups, whereas bacterial community composition differed significantly for both Bray–Curtis (R² = 0.093, p = 0.001) and weighted UniFrac (R² = 0.117, p = 0.006) distances, with no significant differences in multivariate dispersion. At the genus level, Mannheimia showed significantly higher relative abundance in pneumonic lungs after false-discovery-rate correction and was the only genus also identified as differentially abundant by ANCOM. Mycoplasma, Fusobacterium, and Trueperella showed greater relative representation in pneumonic lungs, whereas Acinetobacter and Psychrobacter showed the opposite pattern. Prevalence analysis further demonstrated contrasting taxon distributions between groups. Overall, naturally occurring pneumonia was associated with distinct alterations in lung bacterial community composition rather than a generalised loss of within-sample diversity, with Mannheimia representing the strongest pneumonia-associated genus-level signal.

Background: This study was conducted to evaluate the relationship between oxidative stress and some blood parameters in different number of quarters affected with and without sub-clinical mastitis (SM). Methods: A total of 50 lactating Holstein dairy cows were used in the study. The study groups were as follows; Group 1 (negative test results and no SM), Group 2 (SM in one quarter), Group 3 (SM in two quarters), Group 4 (SM in three quarters) Group 5 (SM in four quarters). Milk samples were obtained from the infected quarters for both Somatic Cell Count (SCC) measurement and bacteriological growth/bacterial identification. Blood was collected for analysis of oxidative stress parameters (total oxidative capacity and total antioxidant capacity) and some blood parameters (glucose, total cholesterol, total bilirubin, aspartate aminotransferase [AST], alanine aminotransferase [ALT]). Result: Somatic Cell Count (SCC) was significantly lower in Group 1 than in Groups 2, 3, 4 and 5 (P less than 0.001). Glucose and total cholesterol levels were significantly higher in Group 5 than in Groups 1, 2, 3 and 4 (P less than 0.001). A significant difference was determined between all groups in respect of AST and ALT activities (P less than 0.001). The Total Oxidative Capacity (TOC) and oxidative stress index value (OSI) levels were significantly lower in Group 1 than in Groups 2, 3, 4 and 5 (P less than 0.001), while Total Antioxidant Capacity (TAC) levels were significantly higher (P less than 0.001). In conclusion, it was found that oxidative stress and blood-biochemistry values are significantly affected in dairy cows with sub-clinical mastitis.