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Aquaculture is one of the fastest-growing economic activities worldwide; shrimp production by aquaculture is around 70% or more of the total consumed. The development of this activity is inducing great benefits in the production of food and jobs; however, shrimp aquaculture is also generating; (1) ecological imbalance by pelagic species overexploitation to produce fish ingredients, (2) bays contamination by inappropriate waste management and (3) pathogens proliferation by antibiotics abuse. In this sense, a significant number of regulations and legal restrictions have been imposed; thus, aquaculture is no longer considered a profitable activity. Therefore, significant and innovative technologies need to be applied to ensure the sustainability and profitability of this activity. In this sense, probiotic bacteria are being used in aquaculture to improve feed intake, modulate gut microbiota and control pathogen proliferation. This work summarizes the results from researchers who worked extensively to show how probiotic bacteria can improve shrimp aquaculture development.
Jorge Olmos Soto. Feed intake improvement, gut microbiota modulation and pathogens control by using Bacillus species in shrimp aquaculture. World Journal of Microbiology and Biotechnology 2021, 37, 1 -7.
AMA StyleJorge Olmos Soto. Feed intake improvement, gut microbiota modulation and pathogens control by using Bacillus species in shrimp aquaculture. World Journal of Microbiology and Biotechnology. 2021; 37 (2):1-7.
Chicago/Turabian StyleJorge Olmos Soto. 2021. "Feed intake improvement, gut microbiota modulation and pathogens control by using Bacillus species in shrimp aquaculture." World Journal of Microbiology and Biotechnology 37, no. 2: 1-7.
The high mortality rate of breast cancer is mainly caused by the metastatic ability of cancer cells, resistance to chemotherapy and radiotherapy, and tumor regression capacity. In recent years, it has been shown that the presence of breast cancer stem cells is closely associated with the migration and metastatic ability of cancer cells, as well as with their resistance to chemotherapy and radiotherapy. The tumor microenvironment is one of the main molecular factors involved in cancer and metastatic processes development, in this sense it is interesting to study the role of platelets, one of the main communicator cells in the human body which are activated by the signals they receive from the microenvironment and can generate more than one response. Platelets can ingest and release RNA, proteins, cytokines and growth factors. After the platelets interact with the tumor microenvironment, they are called "tumor-educated platelets." Tumor-educated platelets transport material from the tumor microenvironment to sites adjacent to the tumor, thus helping to create microenvironments conducive for the development of primary and metastatic tumors. It has been observed that the clone capable of carrying out the metastatic process is a cancer cell with stem cell characteristics. Cancer stem cells go through a series of processes, including epithelial-mesenchymal transition, intravasation into blood vessels, movement through blood vessels, extravasation at the site of the establishment of a metastatic focus, and site colonization. Tumor-educated platelets support all these processes.
Gretel Mendoza-Almanza; Luis Burciaga-Hernández; Vilma Maldonado; Jorge Melendez-Zajgla; Jorge Olmos. Role of platelets and breast cancer stem cells in metastasis. World Journal of Stem Cells 2020, 12, 1237 -1254.
AMA StyleGretel Mendoza-Almanza, Luis Burciaga-Hernández, Vilma Maldonado, Jorge Melendez-Zajgla, Jorge Olmos. Role of platelets and breast cancer stem cells in metastasis. World Journal of Stem Cells. 2020; 12 (11):1237-1254.
Chicago/Turabian StyleGretel Mendoza-Almanza; Luis Burciaga-Hernández; Vilma Maldonado; Jorge Melendez-Zajgla; Jorge Olmos. 2020. "Role of platelets and breast cancer stem cells in metastasis." World Journal of Stem Cells 12, no. 11: 1237-1254.
Bacillus thuringiensis (Bt) is a ubiquitous bacterium in soils, insect cadavers, phylloplane, water, and stored grain, that produces several proteins, each one toxic to different biological targets such as insects, nematodes, mites, protozoa, and mammalian cells. Most Bt toxins identify their particular target through the recognition of specific cell membrane receptors. Cry proteins are the best-known toxins from Bt and a great amount of research has been published. Cry are cytotoxic to insect larvae that affect important crops recognizing specific cell membrane receptors such as cadherin, aminopeptidase-N, and alkaline phosphatase. Furthermore, some Cry toxins such as Cry4A, Cry4B, and Cry11A act synergistically with Cyt toxins against dipteran larvae vectors of human disease. Research developed with Cry proteins revealed that these toxins also could kill human cancer cells through the interaction with specific receptors. Parasporins are a small group of patented toxins that may or may not have insecticidal activity. These proteins could kill a wide variety of mammalian cancer cells by recognizing specific membrane receptors, just like Cry toxins do. Surface layer proteins (SLP), unlike the other proteins produced by Bt, are also produced by most bacteria and archaebacteria. It was recently demonstrated that SLP produced by Bt could interact with membrane receptors of insect and human cancer cells to kill them. Cyt toxins have a structure that is mostly unrelated to Cry toxins; thereby, other mechanisms of action have been reported to them. These toxins affect mainly mosquitoes that are vectors of human diseases like Anopheles spp (malaria), Aedes spp (dengue, zika, and chikungunya), and Culex spp (Nile fever and Rift Valley fever), respectively. In addition to the Cry, Cyt, and parasporins toxins produced during spore formation as inclusion bodies, Bt strains also produce Vip (Vegetative insecticidal toxins) and Sip (Secreted insecticidal proteins) toxins with insecticidal activity during their vegetative growth phase.
Gretel Mendoza-Almanza; Edgar L. Esparza-Ibarra; Jorge L. Ayala-Luján; Marisa Mercado-Reyes; Susana Godina-González; Marisa Hernández-Barrales; Jorge Olmos-Soto. The Cytocidal Spectrum of Bacillus thuringiensis Toxins: From Insects to Human Cancer Cells. Toxins 2020, 12, 301 .
AMA StyleGretel Mendoza-Almanza, Edgar L. Esparza-Ibarra, Jorge L. Ayala-Luján, Marisa Mercado-Reyes, Susana Godina-González, Marisa Hernández-Barrales, Jorge Olmos-Soto. The Cytocidal Spectrum of Bacillus thuringiensis Toxins: From Insects to Human Cancer Cells. Toxins. 2020; 12 (5):301.
Chicago/Turabian StyleGretel Mendoza-Almanza; Edgar L. Esparza-Ibarra; Jorge L. Ayala-Luján; Marisa Mercado-Reyes; Susana Godina-González; Marisa Hernández-Barrales; Jorge Olmos-Soto. 2020. "The Cytocidal Spectrum of Bacillus thuringiensis Toxins: From Insects to Human Cancer Cells." Toxins 12, no. 5: 301.
Beneficial microorganisms maintain the ecosystems, plants, animals and humans working in healthy conditions. In nature, around 95% of all microorganisms produce beneficial effects by increasing nutrients digestion and assimilation, preventing pathogens development and by improving environmental parameters. However, increase in human population and indiscriminate uses of antibiotics have been exerting a great pressure on agriculture, livestock, aquaculture, and also to the environment. This pressure has induced the decomposition of environmental parameters and the development of pathogenic strains resistant to most antibiotics. Therefore, all antibiotics have been restricted by corresponding authorities; hence, new and healthy alternatives to prevent or eliminate these pathogens need to be identified. Thus, probiotic bacteria utilization in aquaculture systems has emerged as a solution to prevent pathogens development, to enhance nutrients assimilation and to improve environmental parameters. In this sense, B. subtilis is an ideal multifunctional probiotic bacterium, with the capacity to solve these problems and also to increase aquaculture profitability.
Jorge Olmos; Manuel Acosta; Gretel Mendoza; Viviana Pitones. Bacillus subtilis, an ideal probiotic bacterium to shrimp and fish aquaculture that increase feed digestibility, prevent microbial diseases, and avoid water pollution. Archives of Microbiology 2019, 202, 427 -435.
AMA StyleJorge Olmos, Manuel Acosta, Gretel Mendoza, Viviana Pitones. Bacillus subtilis, an ideal probiotic bacterium to shrimp and fish aquaculture that increase feed digestibility, prevent microbial diseases, and avoid water pollution. Archives of Microbiology. 2019; 202 (3):427-435.
Chicago/Turabian StyleJorge Olmos; Manuel Acosta; Gretel Mendoza; Viviana Pitones. 2019. "Bacillus subtilis, an ideal probiotic bacterium to shrimp and fish aquaculture that increase feed digestibility, prevent microbial diseases, and avoid water pollution." Archives of Microbiology 202, no. 3: 427-435.
Background:Bacillus thuringiensis toxins are effective against multiple biological targets such as insects, nematodes, mites, protozoa, and importantly, human cancer cells. One of the main mechanisms by which Cry toxins to trigger cell death is the specific recognition of cadherin-like membrane cell receptors.Objective:This work aimed to assess the cytotoxicity of the Cry1Ab and Cry1Ac toxins from Bacillus thuringiensis in HeLa, cervical cancer cell line, as well as their antitumor activity in mouse models.Methods:We analyzed several biological targets of Cry1Ab and Cry1Ac including erythrocytes, insect larvae, as well as cancer and non-cancer cell lines. The viability of HeLa, SiHa, MCF7 and HaCat cells was assessed by MTT 24 h after the administration of Cry toxins. We also studied apoptosis as a possible cytotoxicity mechanism in HeLa. The capacity of Cry toxins to eliminate tumors in xenograft mouse models was also analyzed.Results:Both toxins, Cry1Ab and Cry1Ac, showed specific cytotoxic activity in HeLa (HPV18+) cervical cancer cell line, with a Cry1Ab LC50 of 2.5 µg/ml, and of 0.5 µg/ml for Cry1Ac. Apoptosis was differentially induced in HeLa cells using the same concentration of Cry1Ab and Cry1Ac toxins. Cry1Ac eliminated 50% of the tumors at 10 µg/ml, and eliminate 100% of the tumors at 30 and 50 µg/ml.Conclusion:Bacillus thuringiensis Cry1A toxins show dual cytotoxic activity, in insects as well as in HeLa cancer cell line.
Gretel Mendoza-Almanza; Leticia Rocha-Zavaleta; Cecilia Aguilar-Zacarías; Jorge Ayala-Luján; Jorge Olmos. Cry1A Proteins are Cytotoxic to HeLa but not to SiHa Cervical Cancer Cells. Current Pharmaceutical Biotechnology 2019, 20, 1018 -1027.
AMA StyleGretel Mendoza-Almanza, Leticia Rocha-Zavaleta, Cecilia Aguilar-Zacarías, Jorge Ayala-Luján, Jorge Olmos. Cry1A Proteins are Cytotoxic to HeLa but not to SiHa Cervical Cancer Cells. Current Pharmaceutical Biotechnology. 2019; 20 (12):1018-1027.
Chicago/Turabian StyleGretel Mendoza-Almanza; Leticia Rocha-Zavaleta; Cecilia Aguilar-Zacarías; Jorge Ayala-Luján; Jorge Olmos. 2019. "Cry1A Proteins are Cytotoxic to HeLa but not to SiHa Cervical Cancer Cells." Current Pharmaceutical Biotechnology 20, no. 12: 1018-1027.
Cervical cancer (CC) is one of the leading causes of cancer-associated mortalities in women from developing countries. Similar to other types of cancer, CC is considered to be a multifactorial disease, involving socioeconomic, cultural, immunological and epigenetic factors, as well as persistent human papilloma virus (HPV) infection. It has been well established that cancer stem cells (CSCs) play an important role in defining tumor size, the speed of development and the level of regression following treatment; therefore, CSCs are associated with a poor prognosis. CSCs have been detected in many types of cancer, including leukemia, pancreatic, colon, esophagus, liver, prostate, breast, gastric and lung cancer. In cervical cancer, CSCs have been associated with resistance to normally used drugs such as cisplatin. The present review summarizes the strategies that high-risk HPV viruses (HPV-16 and HPV-18) have developed to transform normal epithelial cells into cancer cells, as well as the cellular pathways and studies associated with the identification of cervical cancer stem cell biomarkers. In this sense, the present review provides state of the art information regarding CC development.
Gretel Mendoza; Leticia Rocha‑Zavaleta; Edgar Esparza‑Ibarra; Jorge Olmos. Cervical cancer stem cells and other leading factors associated with cervical cancer development (Review). Oncology Letters 2019, 18, 3423 -3432.
AMA StyleGretel Mendoza, Leticia Rocha‑Zavaleta, Edgar Esparza‑Ibarra, Jorge Olmos. Cervical cancer stem cells and other leading factors associated with cervical cancer development (Review). Oncology Letters. 2019; 18 (4):3423-3432.
Chicago/Turabian StyleGretel Mendoza; Leticia Rocha‑Zavaleta; Edgar Esparza‑Ibarra; Jorge Olmos. 2019. "Cervical cancer stem cells and other leading factors associated with cervical cancer development (Review)." Oncology Letters 18, no. 4: 3423-3432.
Exploitation of marine fishes is the main source of several life-supporting feed compounds such as proteins, lipids, and carbohydrates that maintain the production of most trading marine organisms by aquaculture. However, at this rate the marine inventory will go to the end soon, since fishery resources are finite. In this sense, the availability of the principal ingredients obtained from marine fishes is going to decrease considerably, increasing the diet prices and affecting the economy of this activity. Therefore, aquaculture industry needs to find nonexpensive land unconventional resources of protein, carbohydrates, and lipids and use bacterial probiotics to improve digestion-assimilation of these unfamiliar compounds. Bacillus subtilis is a cosmopolitan probiotic bacterium with a great enzymatic profile that could improve nutrient digestion-assimilation, induce healthy growth, and avoid water pollution, decreasing economic problems and increasing yields in the aquaculture industry. In this chapter, we present how Bacillus enzymes can help marine animals to assimilate nutrients from unconventional and economic plant resources.
Jorge Olmos Soto. Bacillus Probiotic Enzymes. Advances in Food and Nutrition Research 2017, 80, 15 -35.
AMA StyleJorge Olmos Soto. Bacillus Probiotic Enzymes. Advances in Food and Nutrition Research. 2017; 80 ():15-35.
Chicago/Turabian StyleJorge Olmos Soto. 2017. "Bacillus Probiotic Enzymes." Advances in Food and Nutrition Research 80, no. : 15-35.
Microalgae are present in all aquatic ecosystems on the planet; they are a large group of photosynthetic unicellular eukaryotes and prokaryotes, and its diversity is even larger than superior plants. Around 30 000 have been studied and analyzed, but it is estimated that more than 350 000 species exist. Microalgae produce a great variety of compounds, among which are polysaccharides, lipids, proteins, carotenoids, pigments, vitamins, sterols, enzymes, antibiotics, pharmaceuticals and some fine chemicals, as well as biofuels. In the context of the enormous biodiversity and the development in genetic engineering, microalgae represent one of the most promising biotechnological resources for the isolation of new products and for process development. Among current commercial applications, there are five strains: two cyanobacteria, Arthrospira (Spirulina) and Aphanizomenon flos‐aquae; and three chlorophytas: Chlorella, Dunaliella, and Haematococcus. This chapter highlights the Chlamydomonadales and Chlorellales order as they contain the most known genera of microalgae and a large amount of species to study and exploit.
Rosalia Contreras; J. Paniagua-Michel; Jorge Olmos. Identification of Microalgae Producers of Commercially Important Compounds. Marine Algae Extracts 2015, 41 -58.
AMA StyleRosalia Contreras, J. Paniagua-Michel, Jorge Olmos. Identification of Microalgae Producers of Commercially Important Compounds. Marine Algae Extracts. 2015; ():41-58.
Chicago/Turabian StyleRosalia Contreras; J. Paniagua-Michel; Jorge Olmos. 2015. "Identification of Microalgae Producers of Commercially Important Compounds." Marine Algae Extracts , no. : 41-58.
In addition, Bacillus subtilis (Bs ) is generally recognized as safe (GRAS ) by the Food and Drug Administration (FDA ), meaning that it is harmless to animal and humans. In this work, we present the potential benefits of the utilization of FF in the aquaculture industry.
Jorge Olmos Soto; José De Jesús Paniagua-Michel; Lus M Lopez; Leonel Ochoa. Functional Feeds in Aquaculture. Hb25_Springer Handbook of Marine Biotechnology 2015, 1303 -1319.
AMA StyleJorge Olmos Soto, José De Jesús Paniagua-Michel, Lus M Lopez, Leonel Ochoa. Functional Feeds in Aquaculture. Hb25_Springer Handbook of Marine Biotechnology. 2015; ():1303-1319.
Chicago/Turabian StyleJorge Olmos Soto; José De Jesús Paniagua-Michel; Lus M Lopez; Leonel Ochoa. 2015. "Functional Feeds in Aquaculture." Hb25_Springer Handbook of Marine Biotechnology , no. : 1303-1319.
In the twenty-first century, energy is considered as the most important issue for human sustainability. The world’s dependence on unsustainable fossil fuels (almost 90%Open image in new window), and the increasing population demand new sources of energy for sustainable human activities. Algae, and particularly microalgae have nowadays become of enormous importance as a new potential source of feedstock for renewable bioenergy production. As photosynthetic microorganisms, microalgae may potentially be produced as carbon neutral and can be produced on non-arable land and cultured in marine and wastewater effluents. Furthermore, microalgae can be used to produce a range of products such as protein-rich animal feed in aquaculture, high-value products, viz, polyunsaturated fatty acids, bioactive and functional pigments and natural dyes, health foods, cosmetics, and pharmaceuticals. In this chapter, an update of the advances in microalgal biotechnology is presented as a new biomass for the potential development of biofuels, and as a realistic source of highly valuable molecules of industrial interest. The potential to harness endogenous carbon storage compounds, triacylglyceride (TAG s) and starch, as products of photosynthesis, including the photoproduction of hydrogen, can contribute to diversify the sources and yields of feedstocks for biofuel production. Even if the production of microalgae for biofuels is highly promissory and clearly has potential for contributing to environmental, social, and economic sustainability, presently this alternative is unsustainable. Definitely, the combination of biofuel production by microalgal biotechnology with co-products may contribute to the sustainability of biofuels, a condition with null or less impact on natural resources and biodiversity. The integration of all the components of the uses of microalgae, i. e., high-value compounds, aquaculture, and bioremediation coupled to the production of biofuels will play an important role in the near future to make the production of biofuels from microalgae sustainable. The integration of genomics, metabolic engineering, nanotechnology, and other areas to the aforementioned issues shall lead to a wide range of benefits for the tasks demanded by the forthcoming bioenergy industries.
José De Jesús Paniagua-Michel; Eduardo Morales-Guerrero; Jorge Olmos Soto. Microalgal Biotechnology: Biofuels and Bioproducts. Hb25_Springer Handbook of Marine Biotechnology 2015, 1355 -1370.
AMA StyleJosé De Jesús Paniagua-Michel, Eduardo Morales-Guerrero, Jorge Olmos Soto. Microalgal Biotechnology: Biofuels and Bioproducts. Hb25_Springer Handbook of Marine Biotechnology. 2015; ():1355-1370.
Chicago/Turabian StyleJosé De Jesús Paniagua-Michel; Eduardo Morales-Guerrero; Jorge Olmos Soto. 2015. "Microalgal Biotechnology: Biofuels and Bioproducts." Hb25_Springer Handbook of Marine Biotechnology , no. : 1355-1370.
Litopenaeus vannamei do not have an adaptive immune response system. White spot syndrome virus (WSSV) is the most severe pathogen in shrimps. Bacillus subtilis spores carrying heterologous antigens on its surface have been evaluated as a vaccine inducing specific systemic responses on vertebrates. Orally administrated Vp28 vaccines have been investigated in crustaceans. Vp26 is also an important constituent of WSSV structure but little is known about its oral vaccination capacity in L. vannamei. In this study, for first time, L. vannamei WSSV protection was carried out using B. subtilis recombinant spores (RS), displaying CotC::Vp26 fusion protein (FP) on its surface. RS‐expressing FP were coated on shrimp food pellets and used to feed L. vannamei. Results have shown that orally administered B. subtilis RS protected 100% L. vannamei against WSSV infection. Bacillus subtilis spores orally administrated expressing CotC::Vp26 fusion protein on its surface demonstrated the great capacity of Vp26 to induce immune protection, equally or even greater than Vp28 in L. vannamei. The biotechnological process developed represents an easy to produce, practical to handle, environmentally stable, human‐safe and economically feasible opportunity to apply a new Vp26 vaccine in a massively way in shrimp farms.
A. Valdez; G. Yepiz-Plascencia; Ezio Ricca; J. Olmos. First Litopenaeus vannamei WSSV 100% oral vaccination protection using CotC::Vp26 fusion protein displayed on Bacillus subtilis spores surface. Journal of Applied Microbiology 2014, 117, 347 -357.
AMA StyleA. Valdez, G. Yepiz-Plascencia, Ezio Ricca, J. Olmos. First Litopenaeus vannamei WSSV 100% oral vaccination protection using CotC::Vp26 fusion protein displayed on Bacillus subtilis spores surface. Journal of Applied Microbiology. 2014; 117 (2):347-357.
Chicago/Turabian StyleA. Valdez; G. Yepiz-Plascencia; Ezio Ricca; J. Olmos. 2014. "First Litopenaeus vannamei WSSV 100% oral vaccination protection using CotC::Vp26 fusion protein displayed on Bacillus subtilis spores surface." Journal of Applied Microbiology 117, no. 2: 347-357.
Carbohydrates (CHOs) are the most abundant organic compounds found in living organisms and are a great source of metabolic energy, both for plants and animals. Besides of CHOs great potential to solve animal's energy requirements and diminishing high feed cost, we first must to understand its digestibility and assimilation to avoid several inconvenients. Today, CHOs feed animal inclusions are of great concern about cost-benefits, animal's health status, and environmental pollution. In this chapter, we make a brief description about sugars (DP1-2), oligosaccharides (DP3-9), polysaccharides (DP ≥10), and their essential characteristics to understand the role of marine and terrestrial CHOs in animal nutrition. Subsequently, we talk about basic concepts, CHOs functional benefits, suggestions about their application and successful cases. This information will contribute to produce a new generation of high-quality and energetic functional feed formulations for livestock and aquaculture farms; which must be of low cost, healthy, and environmentally friendly, with the inclusion of prebiotics and probiotics.
Leonel Ochoa; José De Jesús Paniagua Michel; Jorge Olmos-Soto. Complex Carbohydrates as a Possible Source of High Energy to Formulate Functional Feeds. Advances in Food and Nutrition Research 2014, 73, 259 -288.
AMA StyleLeonel Ochoa, José De Jesús Paniagua Michel, Jorge Olmos-Soto. Complex Carbohydrates as a Possible Source of High Energy to Formulate Functional Feeds. Advances in Food and Nutrition Research. 2014; 73 ():259-288.
Chicago/Turabian StyleLeonel Ochoa; José De Jesús Paniagua Michel; Jorge Olmos-Soto. 2014. "Complex Carbohydrates as a Possible Source of High Energy to Formulate Functional Feeds." Advances in Food and Nutrition Research 73, no. : 259-288.
Her-2 gene amplification and its overexpression in breast cancer cells is directly associated with aggressive clinical behavior. The her-2 gene and its Her-2 protein have been utilized for disease diagnosis and as a predictive marker for treatment response to the antibody herceptin. Fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) are the most common FDA-approved methodologies involving gene and protein quantification, respectively. False positive or negative her-2/Her-2 patient results may result in inappropriate treatment administration. To support accurate quantification and interpretation of results, in this study we have standardized qPCR analysis using previously identified IHC samples, obtaining very significant and clinically useful results.
Gretel Mendoza; Amelia Portillo; Jorge Olmos-Soto. Accurate breast cancer diagnosis through real-time PCR her-2 gene quantification using immunohistochemically-identified biopsies. Oncology Letters 2012, 5, 295 -298.
AMA StyleGretel Mendoza, Amelia Portillo, Jorge Olmos-Soto. Accurate breast cancer diagnosis through real-time PCR her-2 gene quantification using immunohistochemically-identified biopsies. Oncology Letters. 2012; 5 (1):295-298.
Chicago/Turabian StyleGretel Mendoza; Amelia Portillo; Jorge Olmos-Soto. 2012. "Accurate breast cancer diagnosis through real-time PCR her-2 gene quantification using immunohistochemically-identified biopsies." Oncology Letters 5, no. 1: 295-298.
Dunaliella salina has become the most important microorganism for the production of β-carotene around the world. Natural carotenoids are a source of active metabolites utilized in different areas of food nutrition and pharmaceuticals, both in humans and also in animals. Identification of Dunaliella species from natural environments or certified culture collections is not precise and it is time consuming. However, accurate identification is extremely important because a slight difference in Dunaliella species generates great differences in carotenoids production. Here, we describe an intron-sizing method to make a rapid and precise identification for each of the most important carotenogenic species, showing that each hyperproducer species has an exclusive 18S rDNA fingerprint profile.
Jorge Olmos-Soto; J. Paniagua-Michel; Rosalía Contreras; Leonel Ochoa. DNA Fingerprinting Intron-Sizing Method to Accomplish a Specific, Rapid, and Sensitive Identification of Carotenogenic Dunaliella Species. Methods in Molecular Biology 2012, 892, 269 -281.
AMA StyleJorge Olmos-Soto, J. Paniagua-Michel, Rosalía Contreras, Leonel Ochoa. DNA Fingerprinting Intron-Sizing Method to Accomplish a Specific, Rapid, and Sensitive Identification of Carotenogenic Dunaliella Species. Methods in Molecular Biology. 2012; 892 ():269-281.
Chicago/Turabian StyleJorge Olmos-Soto; J. Paniagua-Michel; Rosalía Contreras; Leonel Ochoa. 2012. "DNA Fingerprinting Intron-Sizing Method to Accomplish a Specific, Rapid, and Sensitive Identification of Carotenogenic Dunaliella Species." Methods in Molecular Biology 892, no. : 269-281.
Functional feed supplemented with alternative-economic nutrient sources (protein, carbohydrates, lipids) and probiotics are being considered in shrimp/fish aquaculture production systems as an option to increase yield and profits and to reduce water pollution. In this study the probiotic potential to formulate functional feeds have been evaluated using four dietary treatments: Treatment 1 (B + Bs); Bacillus subtilis potential probiotic strain was supplemented to a soybeanmeal (SBM)—carbohydrates (CHO) basal feed. Treatment 2 (B + Bm); Bacillus megaterium potential probiotic strain was supplemented to the same SBM-CHO basal feed. In Treatment 3 (B); SBM-CHO basal feed was not supplemented with probiotic strains. Treatment 4 (C); fishmeal commercial feed (FM) was utilized as positive control. Feeding trials evaluated the survival, growth, and food conversion ratio and stress tolerance of juvenile Litopenaeus vannamei (Boone) Pacific white shrimp. Best overall shrimp performance was observed for animals fed with Treatment 1 (B+Bs); additionally, stress tolerance and hemolymph metabolites also showed the best performance in this treatment. SBM-CHO basal feed not supplemented with probiotic strains (B) presented smaller growth and lower feed conversion ratio (FCR). Shrimps fed with the fishmeal commercial feed (C) presented the lowest stress tolerance to high ammonia and low oxygen levels. Specifically selected B. subtilis strains are recommended to formulate functional and economical feeds containing high levels of vegetable; protein and carbohydrates as main dietary sources in L. vannamei cultures.
Jorge Olmos; Leonel Ochoa; Jesus Paniagua-Michel; Rosalia Contreras. Functional Feed Assessment on Litopenaeus vannamei Using 100% Fish Meal Replacement by Soybean Meal, High Levels of Complex Carbohydrates and Bacillus Probiotic Strains. Marine Drugs 2011, 9, 1119 -1132.
AMA StyleJorge Olmos, Leonel Ochoa, Jesus Paniagua-Michel, Rosalia Contreras. Functional Feed Assessment on Litopenaeus vannamei Using 100% Fish Meal Replacement by Soybean Meal, High Levels of Complex Carbohydrates and Bacillus Probiotic Strains. Marine Drugs. 2011; 9 (6):1119-1132.
Chicago/Turabian StyleJorge Olmos; Leonel Ochoa; Jesus Paniagua-Michel; Rosalia Contreras. 2011. "Functional Feed Assessment on Litopenaeus vannamei Using 100% Fish Meal Replacement by Soybean Meal, High Levels of Complex Carbohydrates and Bacillus Probiotic Strains." Marine Drugs 9, no. 6: 1119-1132.
Dunaliella salina is the most important species of the genus for β-carotene production. Several investigations have demonstrated that D. salina produces more than 10% dry weight of pigment and that the species grows in salt saturated lagoons. High plasticity in the green stage and the almost indistinguishable differences in the red phase make identification and differentiation of species and ecotypes very difficult and time consuming.
Jorge Olmos; Leonel Ochoa; Jesus Paniagua-Michel; Rosalía Contreras. DNA fingerprinting differentiation between β-carotene hyperproducer strains of Dunaliella from around the world. Saline Systems 2009, 5, 5 -5.
AMA StyleJorge Olmos, Leonel Ochoa, Jesus Paniagua-Michel, Rosalía Contreras. DNA fingerprinting differentiation between β-carotene hyperproducer strains of Dunaliella from around the world. Saline Systems. 2009; 5 (1):5-5.
Chicago/Turabian StyleJorge Olmos; Leonel Ochoa; Jesus Paniagua-Michel; Rosalía Contreras. 2009. "DNA fingerprinting differentiation between β-carotene hyperproducer strains of Dunaliella from around the world." Saline Systems 5, no. 1: 5-5.
The bacterial composition in the hatchery at Unidad Experimental Peñasco (UEP) of the Sonora University, Mexico, was studied by using Fluorescent In Situ Hybridization (FISH) with rRNA-specific oligonucleotide probes. We applied fluorochrome-labeled polyribonucleotide probes to identify and enumerate marine shrimp culture hatchery related bacteria. Quantitative whole-cell hybridization experiments using α-, γ- and δ-Proteobacteria, and high and low G + C Gram-positive bacteria accounted for 20.8 ± 3.4% to 69.3 ± 3.3% of the total 4′,6-diamidino-2-phenylindole (DAPI)-stained cells in most samples. As predicted in a previous study, marine high G + C and γ-Proteobacteria predominated in different shrimp life sub-stages. The elevated percent of high G + C and γ-Proteobacteria, extending from nauplii to mysis stages, suggest that they represent a large and significant fraction of the total picoplankton biomass in Litopenaeus vannamei larval culture.
Antonio T. Garcia; Jorge S. Olmos. Quantification by fluorescent in situ hybridization of bacteria associated with Litopenaeus vannamei larvae in Mexican shrimp hatchery. Aquaculture 2007, 262, 211 -218.
AMA StyleAntonio T. Garcia, Jorge S. Olmos. Quantification by fluorescent in situ hybridization of bacteria associated with Litopenaeus vannamei larvae in Mexican shrimp hatchery. Aquaculture. 2007; 262 (2-4):211-218.
Chicago/Turabian StyleAntonio T. Garcia; Jorge S. Olmos. 2007. "Quantification by fluorescent in situ hybridization of bacteria associated with Litopenaeus vannamei larvae in Mexican shrimp hatchery." Aquaculture 262, no. 2-4: 211-218.
In shrimp cultures, feed represents the most expensive production cost. The quantity and quality of diets are primary factors influencing shrimp growth, nitrogen loading of the culture system and disease proliferation. For these reasons there is an interest in developing ‘environmentally friendly’ feeds for optimal growth. In this study, Bacillus strains were isolated from marine environments. The isolates were qualitatively assayed for proteases, carbohydrolases and lipases using selective media. The selected strains (9b, 31 and 33) were molecularly identified as Bacillus subtilis, B. megaterium and B. megaterium, respectively. Subsequently the strains were grown in an inexpensive culture medium (soybean mineral medium (MMS)). The information generated from the present investigation may contribute towards better feed formulations for shrimp at low cost, including bacterial strains as probiotics.
J. Leonel Ochoa-Solano; Jorge Olmos-Soto. The functional property of Bacillus for shrimp feeds. Food Microbiology 2006, 23, 519 -525.
AMA StyleJ. Leonel Ochoa-Solano, Jorge Olmos-Soto. The functional property of Bacillus for shrimp feeds. Food Microbiology. 2006; 23 (6):519-525.
Chicago/Turabian StyleJ. Leonel Ochoa-Solano; Jorge Olmos-Soto. 2006. "The functional property of Bacillus for shrimp feeds." Food Microbiology 23, no. 6: 519-525.
Bacillus subtilis aprE gene codes for the extracellular protease subtilisin. Its expression is controlled by AbrB, DegU, Hpr, SinI, SinR and Spo0A transition state protein regulators. To determine in vivo the protein-protein interactions among these regulators, we used the LexA-based bacterial genetic two-hybrid system. Our results show homo-dimerization to all the analyzed proteins and hetero-dimerization between SinR-SinI and SinR-Hpr.
Alejandro Sánchez; Jorge Olmos. Bacillus subtilis transcriptional regulators interaction. Biotechnology Letters 2004, 26, 403 -407.
AMA StyleAlejandro Sánchez, Jorge Olmos. Bacillus subtilis transcriptional regulators interaction. Biotechnology Letters. 2004; 26 (5):403-407.
Chicago/Turabian StyleAlejandro Sánchez; Jorge Olmos. 2004. "Bacillus subtilis transcriptional regulators interaction." Biotechnology Letters 26, no. 5: 403-407.
Fifteen environmental samples of Vibrio spp. isolated from healthy and diseased shrimps were tested for pathogenicity to juvenile shrimps. Two isolates, strains Z2 and Z3, were observed to be pathogenic, causing 100% mortality of the target host compared to the control strain Vibrio harveyi ATCC 14126. Environmental and type strains were subjected to molecular characterization by restriction fragment length polymorphism (RFLP) and PCR using primers targeted to different virulence, transcriptional regulator, or quorum sensing genes from V. harveyi. Primers designed for luxN were specific and identified all the environmental strains as V. harveyi. The random amplified polymorphic DNA (RAPD) method was used to differentiate between pathogenic and nonpathogenic strains of V. harveyi. These methodologies allowed us to detect and distinguish strains virulent and avirulent to juvenile shrimp.
J. Olmos. Molecular identification of pathogenic and nonpathogenic strains of Vibrio harveyi using PCR and RAPD. Applied Microbiology and Biotechnology 2003, 63, 722 -727.
AMA StyleJ. Olmos. Molecular identification of pathogenic and nonpathogenic strains of Vibrio harveyi using PCR and RAPD. Applied Microbiology and Biotechnology. 2003; 63 (6):722-727.
Chicago/Turabian StyleJ. Olmos. 2003. "Molecular identification of pathogenic and nonpathogenic strains of Vibrio harveyi using PCR and RAPD." Applied Microbiology and Biotechnology 63, no. 6: 722-727.