The industries are searching for greener alternatives for their productions due to the rising concern about the environment and creation of waste and by-products without industrial utility for that specific line of products. This investigation describes the development of two stable nanostructured lipid carriers (NLCs): one is the formulation of a standard NLC, and the other one is the same NLC formulation associated with a natural deep eutectic solvent (NaDES). The research presents the formulation paths of the NLCs through completeness, which encompass dynamic light scattering (DLS), zeta potential tests, and pH. Transmission electron microscopy (TEM) and confocal microscopy were performed to clarify the morphology. Cytotoxicity tests with zebrafish were realized, and the results are complementary to the in vitro outcomes reached with fibroblast L132 tests by the MTT technique and the zymography test. Infrared spectroscopy and X-ray diffractometry tests elucidated the link between the physicochemical characteristics of the formulation and its behavior and properties. Different cooling techniques were explored to prove the tailorable properties of the NLCs for any industrial applications. In conclusion, the compiled results show the successful formulation of new nanocarriers based on a sustainable, eco-friendly, and highly tailorable technology, which presents low cytotoxic potential.

Plant-derived compounds offer immense therapeutic potential, yet many suffer from limited solubility, instability, and poor bioavailability, restricting their clinical application. Curcumin, a polyphenol extracted from Curcuma longa, is one such molecule, with proven antioxidant and anti-inflammatory properties. To overcome its pharmacokinetic limitations, we developed Jamamina, a sustainable nanostructured lipid carrier (NLC) system incorporating curcumin and a Natural Deep Eutectic Solvent (NaDES) phase composed of malic acid and betaine. The bioinspired formulation, based on Amazonian tucumã butter and jambu oil, achieved high encapsulation efficiency (>80%) and curcumin amorphization, enhancing solubility and colloidal stability. In vitro assays with L132 demonstrated potent antioxidant activity (DPPH), a significant reduction in pro-inflammatory cytokines (TNF-α and IL-6), and upregulation of IL-10. The system also suppressed MMP-2/9 activity and preserved cytoskeletal integrity under oxidative stress. These findings highlight Jamamina as a multifunctional, eco-friendly nanoplatform that enables the pharmacological application of plant-derived curcumin, representing a promising platform for modulating redox balance and investigating inflammation in epithelial-like contexts.

Background: Ultraviolet (UV) radiation, urban pollution and depletion of cutaneous antioxidant reserves are interconnected drivers of photoaging, matrix metalloproteinase (MMP) activation, mitochondrial dysfunction and dermal extracellular matrix degradation. Plant polyphenols can counteract these processes, but topical efficacy is limited by chemical instability, polarity-driven partitioning and a hostile stratum corneum delivery interface. Here we report a green nanostructured lipid carrier co-loading a phenolic-rich jabuticaba (Plinia cauliflora) peel extract and caffeine (NLC-JC), engineered with Amazonian lipids (Astrocaryum murumuru butter and Orbignya phalerata oil) and stabilized by a Natural Deep Eutectic Solvent (NaDES)-like microenvironment. Methods: UPLC profiling was used to determine the polyphenolic composition of the extract. Physicochemical characterization included hydrodynamic diameter and PDI, ζ-potential, lipid matrix organization (XRD) and morphology (TEM), as well as colloidal stability over 30 days. Cytotoxicity was assessed in SH-4 melanocytes (ISO 10993-5) and in zebrafish (Danio rerio) embryos (96-h IC₅₀, GHS acute hazard threshold). Functional antioxidant and photoprotective activity was evaluated by DPPH radical scavenging, and by attenuation of UVC- and H₂O₂-induced anion superoxide generation, alongside assessment of cellular and nuclear morphology under combined oxidative stress. Results: UPLC profiling revealed a polyphenolic fingerprint dominated by ellagic acid (476.8 ± 19.7 µg g⁻¹), quercetin-3-rhamnoside (90.9 ± 4.6 µg g⁻¹), quercetin (83.7 ± 1.4 µg g⁻¹), cyanidin-3-glucoside (73.0 ± 1.2 µg g⁻¹) and protocatechuic acid (68.2 ± 2.2 µg g⁻¹), bioactives with documented anti-MMP, NF-κB-suppressing and ROS-scavenging activities. Optimized NLC-JC showed a hydrodynamic diameter of 56.1 nm (PDI = 0.131), negative ζ-potential, amorphous lipid matrix (XRD) and spherical morphology (TEM, ≈150–200 nm electron-dense particles), with 30-day colloidal stability. NLC-JC exhibited low cytotoxicity in SH-4 melanocytes (IC₅₀ = 2304 μg mL⁻¹), preserving ≥70% viability across the cosmetically relevant range (ISO 10993-5), and a 96-h LC₅₀ of 2013 μg mL⁻¹ in zebrafish (Danio rerio) embryos, three orders of magnitude above the GHS acute hazard threshold (100 mg L⁻¹). Functionally, NLC-JC produced ≈50% DPPH radical scavenging at concentrations comparable to ascorbic acid, attenuated UVC- and H₂O₂-induced anion superoxide generation and preserved cellular and nuclear morphology under combined oxidative stress. Conclusion: These data position NLC-JC as a safe-by-design, multifunctional dermocosmetic platform combining agro-industrial valorization with mechanism-anchored photoprotection.