This page has only limited features, please log in for full access.
Iron excess in tissues results in increased oxidative damage. Among different tissues, the skin can particularly be severely damaged by oxidative stress, as it is exposed not only to endogenous but also directly to exogenous pro-oxidants. The skin is especially vulnerable to harmful oxidative stress. Melatonin and indole-3-propionic acid (IPA), two indole substances, are efficient antioxidants. This study aims to evaluate the potential protective effects of melatonin and IPA against oxidative damage to membrane lipids (lipid peroxidation (LPO)), induced in porcine skin homogenates by the Fenton reaction (Fe2+ + H2O2 → Fe3+ + •OH + OH−) when iron is used in extremely high concentrations. Skin homogenates were incubated in the presence of FeSO4 (2400, 1200, 600, 300, 150 and 75 µM) + H2O2 (5 mM) with/without melatonin or IPA. LPO level (MDA + 4-HDA/mg protein) was measured spectrophotometrically. Melatonin, in its highest used concentration (5.0 mM), prevented FeSO4 (1200 mM)-induced LPO, whereas it was effective in concentrations as low as 2.5 mM against all lower iron concentrations. IPA was protective in concentrations as low as 2.5 mM independently of FeSO4 concentration. In conclusion, melatonin and IPA effectively protect against oxidative damage to membrane lipids induced by high concentrations of iron in porcine skin; therefore, both can be considered pharmacological agents in the case of disorders associated with excessive iron accumulation in the skin.
Aleksandra Rynkowska; Jan Stępniak; Małgorzata Karbownik-Lewińska. Melatonin and Indole-3-Propionic Acid Reduce Oxidative Damage to Membrane Lipids Induced by High Iron Concentrations in Porcine Skin. Membranes 2021, 11, 571 .
AMA StyleAleksandra Rynkowska, Jan Stępniak, Małgorzata Karbownik-Lewińska. Melatonin and Indole-3-Propionic Acid Reduce Oxidative Damage to Membrane Lipids Induced by High Iron Concentrations in Porcine Skin. Membranes. 2021; 11 (8):571.
Chicago/Turabian StyleAleksandra Rynkowska; Jan Stępniak; Małgorzata Karbownik-Lewińska. 2021. "Melatonin and Indole-3-Propionic Acid Reduce Oxidative Damage to Membrane Lipids Induced by High Iron Concentrations in Porcine Skin." Membranes 11, no. 8: 571.
Not only iodine deficiency, but also its excess may contribute to thyroid cancer. Potassium iodate (KIO3), which is broadly used in the salt iodization program, can increase oxidative damage to membrane lipids (lipid peroxidation, LPO) under experimental conditions, with the strongest damaging effect at KIO3 concentration of ~10 mM (corresponding to physiological iodine concentration in the thyroid). Melatonin is an effective antioxidant, which protects against KIO3-induced LPO in the thyroid. This study aimed to compare the protective effects of melatonin, used in the highest achievable in vitro concentration, against KIO3-induced oxidative damage to membrane lipids in various porcine tissues (thyroid, ovary, liver, kidney, brain, spleen, and small intestine). Homogenates were incubated in the presence of KIO3 (20; 15; 10; 7.5; 5.0; 0.0 mM) without/with melatonin (5 mM). The malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA) concentration (LPO index) was measured spectrophotometrically. KIO3 increased the LPO in all examined tissues; in the thyroid, the damaging effect of KIO3 (10; and 7.5 mM) was lower than in other tissues and was not observed for the lowest concentration of 5 mM. Melatonin reduced LPO induced by KIO3 (10, 7.5, and 5 mM) in all tissues, and in the thyroid it was also protective against as high a concentration of KIO3 as 15 mM; the LPO level resulting from KIO3 + melatonin treatment was lower in the thyroid than in other tissues. In conclusion, the thyroid is less sensitive tothe pro-oxidative effects of KIO3 compared to other tissues. The strongest protective effect of melatonin was observed in the thyroid, but beneficial effects were significant also in other tissues. Melatonin should be considered to avoid the potential damaging effects of iodine compounds applied in iodine prophylaxis.
Paulina Iwan; Jan Stepniak; Malgorzata Karbownik-Lewinska. Pro-Oxidative Effect of KIO3 and Protective Effect of Melatonin in the Thyroid—Comparison to Other Tissues. Life 2021, 11, 592 .
AMA StylePaulina Iwan, Jan Stepniak, Malgorzata Karbownik-Lewinska. Pro-Oxidative Effect of KIO3 and Protective Effect of Melatonin in the Thyroid—Comparison to Other Tissues. Life. 2021; 11 (6):592.
Chicago/Turabian StylePaulina Iwan; Jan Stepniak; Malgorzata Karbownik-Lewinska. 2021. "Pro-Oxidative Effect of KIO3 and Protective Effect of Melatonin in the Thyroid—Comparison to Other Tissues." Life 11, no. 6: 592.
Iodine deficiency is the main environmental factor leading to thyroid cancer. At the same time iodine excess may also contribute to thyroid cancer. Potassium iodate (KIO3), which is broadly used in salt iodization program, may increase oxidative damage to membrane lipids (lipid peroxidation, LPO) under experimental conditions, with the strongest damaging effect at KIO3 concentration of ~10 mM (corresponding to physiological iodine concentration in the thyroid). Melatonin and indole-3-propionic acid (IPA) are effective antioxidative indoles, each of which protects against KIO3-induced LPO in the thyroid. The study aims to check if melatonin used together with IPA (in their highest achievable in vitro concentrations) reveals stronger protective effects against KIO3-induced LPO in porcine thyroid homogenates than each of these antioxidants used separately. Homogenates were incubated in the presence of KIO3 (200; 100; 50; 25; 20; 15; 10; 7.5; 5.0; 2.5; 1.25; 0.0 mM) without/with melatonin (5 mM) or without/with IPA (10 mM) or without/with melatonin + IPA, and then, to further clarify the narrow range of KIO3 concentrations, against which melatonin + IPA reveal cumulative protective effects, the following KIO3 concentrations were used: 20; 18.75; 17.5; 16.25; 15; 13.75; 12.5; 11.25; 10; 8.75; 7.5; 0.0 mM. Malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA) concentration (LPO index) was measured spectrophotometrically. Protective effects of melatonin + IPA were stronger than those revealed by each antioxidant used separately, but only when LPO was induced by KIO3 in concentrations from 18.75 mM to 8.75 mM, corresponding to physiological iodine concentration in the thyroid. In conclusion, melatonin and indole-3-propionic acid exert cumulative protective effects against oxidative damage caused by KIO3, when this prooxidant is used in concentrations close to physiological iodine concentrations in the thyroid. Therefore, the simultaneous administration of these two indoles should be considered to prevent more effectively oxidative damage (and thereby thyroid cancer formation) caused by iodine compounds applied in iodine prophylaxis.
Paulina Iwan; Jan Stepniak; Malgorzata Karbownik-Lewinska. Cumulative Protective Effect of Melatonin and Indole-3-Propionic Acid against KIO3—Induced Lipid Peroxidation in Porcine Thyroid. Toxics 2021, 9, 89 .
AMA StylePaulina Iwan, Jan Stepniak, Malgorzata Karbownik-Lewinska. Cumulative Protective Effect of Melatonin and Indole-3-Propionic Acid against KIO3—Induced Lipid Peroxidation in Porcine Thyroid. Toxics. 2021; 9 (5):89.
Chicago/Turabian StylePaulina Iwan; Jan Stepniak; Malgorzata Karbownik-Lewinska. 2021. "Cumulative Protective Effect of Melatonin and Indole-3-Propionic Acid against KIO3—Induced Lipid Peroxidation in Porcine Thyroid." Toxics 9, no. 5: 89.
The aim of the study was to evaluate the distribution of blood dendritic cells (DCs) in patients with Graves’ orbitopathy (GO) and to assess the influence of methylprednisolone therapy on subsets of peripheral blood mononuclear cells (PBMCs). Peripheral blood DC subsets were analyzed by flow cytometry in patients with active GO ( n = 17 ), inactive GO ( n = 8 ), and Graves’ disease (GD) without GO ( n = 8 ) and controls ( n = 15 ); additionally, in patients with active GO ( n = 17 ), analyses were done at three time points, i.e., before methylprednisolone treatment and after 6 weeks and after 12 weeks of the treatment. Percentage of myeloid DCs (mDCs) in PBMC fraction was significantly lower in patients with both active and inactive GO, compared to patients with GD without GO and controls ( p < 0.05 ). In addition, mDCs were also documented to be an independent factor negatively associated with GO, however without essential differences between active and inactive phases. On the other hand, we did not observe any changes in the percentage of DCs after methylprednisolone therapy ( p > 0.05 ). In the present study, we have succeeded to firstly demonstrate—according to our knowledge—that blood mDCs are negatively related to GO incidence.
Katarzyna Wojciechowska-Durczynska; Katarzyna Wieczorek-Szukala; Borys Stefanski; Arkadiusz Zygmunt; Jan Stepniak; Małgorzata Karbownik-Lewinska; Andrzej Lewinski. Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves’ Orbitopathy. Mediators of Inflammation 2021, 2021, 1 -8.
AMA StyleKatarzyna Wojciechowska-Durczynska, Katarzyna Wieczorek-Szukala, Borys Stefanski, Arkadiusz Zygmunt, Jan Stepniak, Małgorzata Karbownik-Lewinska, Andrzej Lewinski. Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves’ Orbitopathy. Mediators of Inflammation. 2021; 2021 ():1-8.
Chicago/Turabian StyleKatarzyna Wojciechowska-Durczynska; Katarzyna Wieczorek-Szukala; Borys Stefanski; Arkadiusz Zygmunt; Jan Stepniak; Małgorzata Karbownik-Lewinska; Andrzej Lewinski. 2021. "Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves’ Orbitopathy." Mediators of Inflammation 2021, no. : 1-8.
The Fenton reaction (Fe2++H2O2→Fe3++•OH+OH-) results in strong oxidative damage to macromolecules when iron (Fe) or hydrogen peroxide (H2O2) are in excess. This study aims at comparing Fe2++H2O2-induced oxidative damage to membrane lipids (lipid peroxidation, LPO) and protective effects of 17β-estradiol (a potential antioxidant) in porcine ovary and thyroid homogenates. Iron, as one of the Fenton reaction substrates, was used in the highest achievable concentrations. Thyroid or ovary homogenates were incubated in the presence of: (1st) FeSO4+H2O2 with/without 17β-estradiol (1 mM; 100, 10.0, 1.0 µM; 100, 10.0, 1.0 nM; 100, 10.0, 1.0 pM); five experiments were performed with different FeSO4 concentrations (2400, 1200, 600, 300, 150 µM); (2nd) FeSO4 (2400, 1200, 600, 300, 150 µM)+H2O2 with/without 17β-estradiol; three experiments were performed with three highest 17β-estradiol concentrations; (3rd) FeSO4 (2400, 1200, 1100, 1000, 900, 800, 700, 600, 300, 150, 75 µM)+H2O2 (5 mM). LPO level [MDA+4-HDA/mg protein] was measured spectrophotometrically. The basal LPO level is lower in ovary than in thyroid homogenates. However, experimentally-induced LPO was higher in the former tissue, which was confirmed for the three highest Fe2+ concentrations (2400, 1200, 1100 µM). Exogenous 17β-estradiol (1 mM, 100, and 10 µM) reduced experimentally-induced LPO independently of iron concentration and that protective effect did not differ between tissues. The ovary, compared to the thyroid, reveals higher sensitivity to prooxidative effects of iron, however, it showed similar responsivity to protective 17β-estradiol activity. The therapeutic effect of 17β-estradiol against iron overload consequences should be considered with relation to both tissues.
Aleksandra Rynkowska; Jan Stępniak; Małgorzata Karbownik-Lewińska. Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates. International Journal of Environmental Research and Public Health 2020, 17, 6841 .
AMA StyleAleksandra Rynkowska, Jan Stępniak, Małgorzata Karbownik-Lewińska. Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates. International Journal of Environmental Research and Public Health. 2020; 17 (18):6841.
Chicago/Turabian StyleAleksandra Rynkowska; Jan Stępniak; Małgorzata Karbownik-Lewińska. 2020. "Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates." International Journal of Environmental Research and Public Health 17, no. 18: 6841.
An amendment to this paper has been published and can be accessed via the original article.
Malgorzata Karbownik-Lewinska; Jan Stepniak; Magdalena Marcinkowska; Adrian Krygier; Andrzej Lewinski. Correction to: High normal TSH is associated with lower mannan-binding lectin in women of childbearing age. BMC Endocrine Disorders 2020, 20, 1 -1.
AMA StyleMalgorzata Karbownik-Lewinska, Jan Stepniak, Magdalena Marcinkowska, Adrian Krygier, Andrzej Lewinski. Correction to: High normal TSH is associated with lower mannan-binding lectin in women of childbearing age. BMC Endocrine Disorders. 2020; 20 (1):1-1.
Chicago/Turabian StyleMalgorzata Karbownik-Lewinska; Jan Stepniak; Magdalena Marcinkowska; Adrian Krygier; Andrzej Lewinski. 2020. "Correction to: High normal TSH is associated with lower mannan-binding lectin in women of childbearing age." BMC Endocrine Disorders 20, no. 1: 1-1.
High-normal TSH can be associated with metabolic abnormalities and infertility. Aims of this study are to analyze retrospectively if routinely measured blood laboratory and anthropometric parameters differ between women of reproductive age with TSH < 2.5 mIU/L and with TSH ≥ 2.5 mIU/L. Retrospective analysis was performed in 466 female inpatients, aged 13–51, hospitalized in an endocrine department. The group of 280 patients with normal thyroid tests (TSH 0.27-4.2 mIU/L; normal FT3 and FT4) was selected and it was divided into two subgroups, i.e., with TSH < 2.5 mIU/L and TSH ≥ 2.5 mIU/L (n = 66; 23.6%). After excluding patients on L-thyroxine treatment (n = 240), those with TSH ≥ 2.5 mIU/L constituted 22.92% (n = 55). In the group of 280 patients with normal thyroid tests, an abnormally high concentration of triglycerides and an abnormally low HDLC/cholesterol ratio occurred more frequently in women with TSH ≥ 2.5 mIU/L than those with TSH < 2.5 mIU/L (17% vs. 7%, p = 0.017; 14% vs. 5%, p = 0.015, respectively). Increased concentration of thyroid antibodies, i.e., TPOAb, occurred more frequently in patients with TSH ≥ 2.5 mIU/L than those with TSH < 2.5 mIU/L (27% vs. 9%, p = 0.001). The same differences were found in the group of 240 patients after excluding those on L-thyroxine treatment. Blood lymphocyte concentration was the only independent linear parameter associated with TSH ≥ 2.5 mIU/L (OR = 1.551, p = 0.024) but only in the group of 280 patients with normal thyroid tests. TSH concentration correlated positively with blood lymphocyte (r = 0.129, p = 0.031) and TPOAb (r = 0.177, p = 0.005) concentrations but only in the group of 280 patients with normal thyroid tests. Less favorable lipid profiles and a higher prevalence of thyroid antibodies in women of reproductive age with high-normal TSH suggests that L-thyroxine treatment should be considered in such patients. The significance of a positive association between high-normal TSH and blood lymphocytes requires further evaluation.
Małgorzata Karbownik-Lewińska; Jan Stępniak; Anna Żurawska; Andrzej Lewiński. Less Favorable Lipid Profile and Higher Prevalence of Thyroid Antibodies in Women of Reproductive Age with High-Normal TSH—Retrospective Study. International Journal of Environmental Research and Public Health 2020, 17, 2122 .
AMA StyleMałgorzata Karbownik-Lewińska, Jan Stępniak, Anna Żurawska, Andrzej Lewiński. Less Favorable Lipid Profile and Higher Prevalence of Thyroid Antibodies in Women of Reproductive Age with High-Normal TSH—Retrospective Study. International Journal of Environmental Research and Public Health. 2020; 17 (6):2122.
Chicago/Turabian StyleMałgorzata Karbownik-Lewińska; Jan Stępniak; Anna Żurawska; Andrzej Lewiński. 2020. "Less Favorable Lipid Profile and Higher Prevalence of Thyroid Antibodies in Women of Reproductive Age with High-Normal TSH—Retrospective Study." International Journal of Environmental Research and Public Health 17, no. 6: 2122.
Background Mannan-binding lectin (MBL) is a main component of the lectin pathway of the complement system. Lower MBL levels are associated with, among other conditions, hypothyroidism and adverse pregnancy outcomes. In turn, adverse pregnancy outcomes and infertility may result from hypothyroidism, even in patients with high normal Thyroid-stimulating hormone (TSH). The aim of this study was to determine if MBL level differs between women of reproductive age with low normal (< 2.5 mIU/l) and high normal (≥2.5 mIU/l) TSH. Associations with other parameters potentially affected by hypothyroidism were also evaluated. Methods Ninety five (95) patients with normal thyroid tests (TSH 0.27–4.2 mIU/l), aged 18–48 years, were prospectively enrolled. Several laboratory parameters were measured, including MBL level, thyroid tests and lipid profile. Results Serum MBL level was lower in women with TSH ≥ 2.5 mIU/l than with TSH < 2.5 mIU/l. This association was confirmed by univariate regression analysis. MBL level was significantly lower in patients with abnormally low HDLC/cholesterol ratio and a positive correlation was found between MBL level and HDL/cholesterol ratio. Conclusion In women of reproductive age with normal thyroid tests, lower MBL is associated with high normal TSH and with less favourable lipid profile. Therefore treatment with L-thyroxine should be considered in women of reproductive age with TSH ≥ 2.5 mIU/l.
Malgorzata Karbownik-Lewinska; Jan Stepniak; Magdalena Marcinkowska; Adrian Krygier; Andrzej Lewinski. High normal TSH is associated with lower mannan-binding lectin in women of childbearing age. BMC Endocrine Disorders 2020, 20, 1 .
AMA StyleMalgorzata Karbownik-Lewinska, Jan Stepniak, Magdalena Marcinkowska, Adrian Krygier, Andrzej Lewinski. High normal TSH is associated with lower mannan-binding lectin in women of childbearing age. BMC Endocrine Disorders. 2020; 20 (1):1.
Chicago/Turabian StyleMalgorzata Karbownik-Lewinska; Jan Stepniak; Magdalena Marcinkowska; Adrian Krygier; Andrzej Lewinski. 2020. "High normal TSH is associated with lower mannan-binding lectin in women of childbearing age." BMC Endocrine Disorders 20, no. 1: 1.
It has long been observed that thyroid diseases are more prevalent in women than in men. However, there are limited experimental data demonstrating mechanisms by which sex differences in thyroid diseases may occur and exact molecular mechanisms involved are still far from clear. The aim of the study was to evaluate if there are sex differences concerning oxidative damage to membrane lipids in thyroid homogenates in response to Fenton reaction substrates, i.e., Fe2+ and/or H2O2, and, additionally, in response to potentially protective agent, i.e., melatonin. Homogenates of male or female thyroids collected from adult swine (Sus scrofa domesticus) at slaughter were incubated in the presence of H2O2 and/or Fe2+ without or with addition of melatonin. Malondialdehyde + 4-hydroxyalkenals concentration (LPO index) was measured spectrophotometrically. Neither H2O2 nor Fe2+, when used separately, did affect the level of lipid peroxidation in both male and female porcine thyroid homogenates. When H2O2 (0.5 mM) was used together with different concentrations of Fe2+, the level of lipid peroxidation increased significantly in both male and female porcine thyroid homogenates, with clear Fe2+ concentration-dependent stimulatory effect, but without differences between sexes. No sex-specific differences was found concerning oxidative damage to membrane lipids in porcine thyroid in response to Fenton reaction substrates and/or to melatonin. The lack of expected differences may be due to potentially lower sensitivity of membrane lipids comparing to other biological macromolecules to pro-/antioxidative agents in the thyroid. However, further studies should be performed to explain the discussed issue.
Jan Stepniak; Andrzej Lewinski; Malgorzata Karbownik-Lewinska. Oxidative damage to membrane lipids in the thyroid – no differences between sexes. Drug and Chemical Toxicology 2019, 1 -6.
AMA StyleJan Stepniak, Andrzej Lewinski, Malgorzata Karbownik-Lewinska. Oxidative damage to membrane lipids in the thyroid – no differences between sexes. Drug and Chemical Toxicology. 2019; ():1-6.
Chicago/Turabian StyleJan Stepniak; Andrzej Lewinski; Malgorzata Karbownik-Lewinska. 2019. "Oxidative damage to membrane lipids in the thyroid – no differences between sexes." Drug and Chemical Toxicology , no. : 1-6.
It has long been observed that females are more susceptible to thyroid diseases than males. Epidemiological and experimental data show that actions of hormonal factors—especially estrogens—may explain such disparity. However, the exact cause and mechanisms of this sexual dimorphism remain so far unknown. Therefore, we aimed at evaluating the effect of 17β-estradiol on the redox balance in thyroids of male and female rats. Expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, i.e., dual oxidase 1 (DUOX1), dual oxidase 2 (DUOX2) and NADPH oxidase 4 (NOX4), and hydrogen peroxide (H2O2) levels were evaluated in the primary cell cultures derived from thyroid glands of adult male or female Wistar rats. The measurement was made before and after treatment with 17β-estradiol alone or with addition of one of its receptor antagonists. We found that under basal conditions female thyroid cells are exposed to higher concentrations of H2O2, most likely due to NOX/DUOX enzymes activity. Additionally, exogenous 17β-estradiol stimulated NOX/DUOX expression as well as H2O2 production, and this effect was mainly mediated through ERα. In conclusion, oxidative processes may constitute mechanisms responsible for sexual dimorphism of thyroid diseases. Exogenous 17β-estradiol may play a crucial pathogenic role in thyroid diseases via oxidative mechanisms, however without any gender differences.
Jan Stepniak; Andrzej Lewinski; Malgorzata Karbownik-Lewinska. Sexual Dimorphism of NADPH Oxidase/H2O2 System in Rat Thyroid Cells; Effect of Exogenous 17β-Estradiol. International Journal of Molecular Sciences 2018, 19, 4063 .
AMA StyleJan Stepniak, Andrzej Lewinski, Malgorzata Karbownik-Lewinska. Sexual Dimorphism of NADPH Oxidase/H2O2 System in Rat Thyroid Cells; Effect of Exogenous 17β-Estradiol. International Journal of Molecular Sciences. 2018; 19 (12):4063.
Chicago/Turabian StyleJan Stepniak; Andrzej Lewinski; Malgorzata Karbownik-Lewinska. 2018. "Sexual Dimorphism of NADPH Oxidase/H2O2 System in Rat Thyroid Cells; Effect of Exogenous 17β-Estradiol." International Journal of Molecular Sciences 19, no. 12: 4063.
Oxidative stress has been implicated in the normal ageing process and the pathogenesis of several diseases, including goitre. The aim of the study was to evaluate the relationship between urine lipid peroxidation (LPO) and anthropometric parameters as well as the parameters associated with goitre formation in children. The subjects included 172 healthy children (93 girls and 79 boys) aged 8–15, divided into 4 age groups – group I (8–9 years), group II (10–11 years), group III (12–13 years) and group IV (14–15 years) – and into 2 groups based on the BSA: the BSA-1 group (≤ 0.55 m2) and the BSA-2 group (> 0.55 m2). The value of LPO was the highest in group I but the difference between the groups was not statistically significant (p = 0.074). In the BSA-1 group, the LPO was higher than in the BSA-2 group (12.75 ±6.90 nmol/ml and 10.79 ±4.86 nmol/ml, respectively; p = 0.023). We found a weak, negative linear correlation between LPO and age (r = –0.216; p < 0.005), body mass (r = –0.153; p < 0.05), height (r = –0.152; p < 0.05) and BSA (r = –0.151; p < 0.05). Anthropometric parameters of school-age children independently of age are negatively associated with oxidative damage to membrane lipids, whereas factors promoting goitrogenesis do not contribute to this process.
Arkadiusz Zygmunt; Jan Stepniak; Katarzyna Wojciechowska-Durczynska; Kinga Krawczyk-Rusiecka; Andrzej Lewiński; Malgorzata Karbownik-Lewinska. Relationship between urine lipid peroxidation, anthropometric parameters and parameters associated with goitre formation in school-age children. Archives of Medical Science 2018, 14, 30 -37.
AMA StyleArkadiusz Zygmunt, Jan Stepniak, Katarzyna Wojciechowska-Durczynska, Kinga Krawczyk-Rusiecka, Andrzej Lewiński, Malgorzata Karbownik-Lewinska. Relationship between urine lipid peroxidation, anthropometric parameters and parameters associated with goitre formation in school-age children. Archives of Medical Science. 2018; 14 (1):30-37.
Chicago/Turabian StyleArkadiusz Zygmunt; Jan Stepniak; Katarzyna Wojciechowska-Durczynska; Kinga Krawczyk-Rusiecka; Andrzej Lewiński; Malgorzata Karbownik-Lewinska. 2018. "Relationship between urine lipid peroxidation, anthropometric parameters and parameters associated with goitre formation in school-age children." Archives of Medical Science 14, no. 1: 30-37.
[b]Abstract Introduction and objective[/b]. Protective antioxidative effects of melatonin have been repeatedly documented in experimental and clinical studies. One of the most spectacular exogenous prooxidative agents is cigarette smoking. The aim of the study was to evaluate the level of...
Dorota Sagan; Jan Stepniak; Adam Gesing; Andrzej Lewinski; Malgorzata Karbownik-Lewinska. Melatonin reverses the enhanced oxidative damage to membrane lipids and improves skin biophysical characteristics in former-smokers – A study in postmenopausal women. Annals of Agricultural and Environmental Medicine 2017, 24, 659 -666.
AMA StyleDorota Sagan, Jan Stepniak, Adam Gesing, Andrzej Lewinski, Malgorzata Karbownik-Lewinska. Melatonin reverses the enhanced oxidative damage to membrane lipids and improves skin biophysical characteristics in former-smokers – A study in postmenopausal women. Annals of Agricultural and Environmental Medicine. 2017; 24 (4):659-666.
Chicago/Turabian StyleDorota Sagan; Jan Stepniak; Adam Gesing; Andrzej Lewinski; Malgorzata Karbownik-Lewinska. 2017. "Melatonin reverses the enhanced oxidative damage to membrane lipids and improves skin biophysical characteristics in former-smokers – A study in postmenopausal women." Annals of Agricultural and Environmental Medicine 24, no. 4: 659-666.
According to current recommendations, a TSH value of <2.5 mIU/l should be maintained during preconception and pregnancy. The same recommendation, however, does not relate to all women of childbearing age. The aim of the study was to evaluate relationship between lipid peroxidation (LPO; index of oxidative damage to membrane lipids) and thyroid tests and other parameters, which may be affected by thyroid dysfunction, in euthyroid women of childbearing age. Ninety nine female inpatients with normal thyroid tests (TSH 0.27–4.2 mIU/l), aged 18–48 years, were prospectively enrolled. Blood concentrations of malondialdehyde+4-hydroxyalkenals (LPO index) were measured spectrophotometrically. Thyroid tests (TSH, FT4, FT3), thyroid antibodies and other laboratory parameters [cholesterol, HDL cholesterol (HDLC), LDL cholesterol, HDLC/cholesterol ratio, triglycerides, glucose, CRP, iron] were measured with standard methods. Blood LPO level was higher in women with TSH≥2.5 mIU/l than in women with TSH<2.5 mIU/l. Positive correlation was found between TSH concentration and LPO level (r=0.210, p=0.037). In the univariate regression analysis, blood LPO level did constitute the only independent factor associated with TSH≥2.5 mIU/l. Abnormal HDLC/cholesterol ratio occurred more frequently in subjects with TSH≥2.5 mIU/l. Additionally, LPO level correlated positively with triglyceride concentration (r=0.340, p=0.001), whereas it correlated negatively with HDLC concentration (r=–0.335, p=0.001) and with HDL/cholesterol ratio (r=–0.331, p=0.001). In conclusion, in women of childbearing age with normal thyroid tests, TSH≥2.5 mIU/l is associated with higher oxidative damage to membrane lipids and less favorable lipid profile, which supports our standpoint that TSH of less than 2.5 mIU/l should be maintained in all women of childbearing age.
Malgorzata Karbownik-Lewinska; Magdalena Marcinkowska; Jan Stepniak; Andrzej Lewinski. TSH ≥2.5 mIU/l is Associated with the Increased Oxidative Damage to Membrane Lipids in Women of Childbearing Age with Normal Thyroid Tests. Hormone and Metabolic Research 2017, 49, 321 -326.
AMA StyleMalgorzata Karbownik-Lewinska, Magdalena Marcinkowska, Jan Stepniak, Andrzej Lewinski. TSH ≥2.5 mIU/l is Associated with the Increased Oxidative Damage to Membrane Lipids in Women of Childbearing Age with Normal Thyroid Tests. Hormone and Metabolic Research. 2017; 49 (5):321-326.
Chicago/Turabian StyleMalgorzata Karbownik-Lewinska; Magdalena Marcinkowska; Jan Stepniak; Andrzej Lewinski. 2017. "TSH ≥2.5 mIU/l is Associated with the Increased Oxidative Damage to Membrane Lipids in Women of Childbearing Age with Normal Thyroid Tests." Hormone and Metabolic Research 49, no. 5: 321-326.
Estrogens, with their principle representative 17β-estradiol, contribute to the redox state of cells showing both pro- and antioxidative properties. In the ovary, being the main source of estrogens, maintaining balance between the production and detoxification of ROS is crucial. Whereas ovary estrogen concentration is difficult to estimate, its circulating concentration in women may reach the nanomolar level. The aim of the study was to evaluate the effects of 17β-estradiol on oxidative damage to membrane lipids (lipid peroxidation, LPO) and to nuclear DNA in the porcine ovary under basal conditions and in the presence of Fenton reaction (Fe(2+)+H2O2→Fe(3+)+(•)OH + OH(-)) substrates. Ovary homogenates and DNA were incubated in the presence of 17β-estradiol (1 mM-1 pM), without/with FeSO4 (30 μM) + H2O2 (0.5 mM). Malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA) concentration (LPO index) was measured spectrophotometrically. The concentration of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) (DNA damage index) was measured by HPLC. We observed that 17β-estradiol did not alter the basal level of oxidative damage, but reduced Fe(2+)+H2O2-induced oxidative damage to membrane lipids when ≥10 nM and to DNA at concentrations ≥1 nM. In the ovary at near physiological concentration, 17β-estradiol prevents experimentally induced oxidative damage. This suggests that under physiological conditions this hormone may contribute to protecting the ovary against oxidative damage.
Jan Stepniak; Malgorzata Karbownik-Lewinska. 17β-estradiol prevents experimentally-induced oxidative damage to membrane lipids and nuclear DNA in porcine ovary. Systems Biology in Reproductive Medicine 2015, 62, 17 -21.
AMA StyleJan Stepniak, Malgorzata Karbownik-Lewinska. 17β-estradiol prevents experimentally-induced oxidative damage to membrane lipids and nuclear DNA in porcine ovary. Systems Biology in Reproductive Medicine. 2015; 62 (1):17-21.
Chicago/Turabian StyleJan Stepniak; Malgorzata Karbownik-Lewinska. 2015. "17β-estradiol prevents experimentally-induced oxidative damage to membrane lipids and nuclear DNA in porcine ovary." Systems Biology in Reproductive Medicine 62, no. 1: 17-21.
Dorota Sagan; Jan Stępniak; Adam Gesing; Andrzej Lewinski; Malgorzata Karbownik-Lewinska. Higher lipid peroxidation in former-smokers vs. never-smokers - study in postmenopausal women. Neuro endocrinology letters 2015, 36, 1 .
AMA StyleDorota Sagan, Jan Stępniak, Adam Gesing, Andrzej Lewinski, Malgorzata Karbownik-Lewinska. Higher lipid peroxidation in former-smokers vs. never-smokers - study in postmenopausal women. Neuro endocrinology letters. 2015; 36 (6):1.
Chicago/Turabian StyleDorota Sagan; Jan Stępniak; Adam Gesing; Andrzej Lewinski; Malgorzata Karbownik-Lewinska. 2015. "Higher lipid peroxidation in former-smokers vs. never-smokers - study in postmenopausal women." Neuro endocrinology letters 36, no. 6: 1.
K. Szokalska; J. Stępniak; M. Karbownik-Lewinska. Lipid peroxidation evaluated in epidermis exfoliated during microdermabrasion is a reliable marker of oxidative stress related to obesity. Journal of the European Academy of Dermatology and Venereology 2015, 30, 1429 -1431.
AMA StyleK. Szokalska, J. Stępniak, M. Karbownik-Lewinska. Lipid peroxidation evaluated in epidermis exfoliated during microdermabrasion is a reliable marker of oxidative stress related to obesity. Journal of the European Academy of Dermatology and Venereology. 2015; 30 (8):1429-1431.
Chicago/Turabian StyleK. Szokalska; J. Stępniak; M. Karbownik-Lewinska. 2015. "Lipid peroxidation evaluated in epidermis exfoliated during microdermabrasion is a reliable marker of oxidative stress related to obesity." Journal of the European Academy of Dermatology and Venereology 30, no. 8: 1429-1431.
Iodine, bivalent iron (Fe²⁺), and hydrogen peroxide (H₂O₂), all significantly affecting the red-ox balance, are required for thyroid hormone synthesis. Intracellular iodine excess (≥10⁻³ M) transiently blocks thyroid hormonogenesis (an adaptive mechanism called Wolff-Chaikoff effect). The aim of the study was to evaluate the effects of iodine, used as potassium iodide (KI) or potassium iodate (KIO₃), in concentrations corresponding to those typical for Wolff-Chaikoff effect, on the level of oxidative damage to nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) isolated from porcine thyroid under basal conditions and in the presence of Fenton reaction (Fe²⁺+H₂O₂ → Fe³⁺+(·)OH + OH⁻) substrates. Thyroid nDNA and mtDNA were incubated in the presence of either KI or KIO₃ (2.5-50 mM), without/with FeSO₄ (30 µM) + H₂O₂ (0.5 mM). Index of DNA damage, i.e., 8-oxo-7,8-dihydro-2'-deoxyguanosine, was measured by HPLC. Neither KI nor KIO₃ increased the basal level of 8-oxodG in both nDNA and mtDNA. KI-in all used concentrations-completely prevented the damaging effect of Fenton reaction substrates in mtDNA, and it partially prevented this damage in nDNA. KIO₃ partially prevented Fe²⁺+H₂O₂-induced oxidative damage in both DNA only in its highest used concentrations (≥25 mM). Without additional prooxidative abuse, both iodine compounds, i.e., KI and KIO₃, seem to be safe in terms of their potential oxidative damage to DNA in the thyroid. The superiority of KI over KIO₃ relies on its stronger protective effects against oxidative damage to mtDNA, which constitutes an argument for its preferential utility in iodine prophylaxis.
Malgorzata Karbownik-Lewinska; Jan Stepniak; Magdalena Milczarek; Andrzej Lewiński. Protective effect of KI in mtDNA in porcine thyroid: comparison with KIO₃ and nDNA. European Journal of Nutrition 2014, 54, 319 -23.
AMA StyleMalgorzata Karbownik-Lewinska, Jan Stepniak, Magdalena Milczarek, Andrzej Lewiński. Protective effect of KI in mtDNA in porcine thyroid: comparison with KIO₃ and nDNA. European Journal of Nutrition. 2014; 54 (2):319-23.
Chicago/Turabian StyleMalgorzata Karbownik-Lewinska; Jan Stepniak; Magdalena Milczarek; Andrzej Lewiński. 2014. "Protective effect of KI in mtDNA in porcine thyroid: comparison with KIO₃ and nDNA." European Journal of Nutrition 54, no. 2: 319-23.
Whereas oxidative reactions occur in all tissues and organs, the thyroid constitutes such an organ, in which oxidative processes are indispensable for physiological functions. In turn, numerous metabolic reactions occurring in the liver create favourable conditions for huge oxidative stress. Melatonin is a well-known antioxidant with protective effects against oxidative damage perfectly documented in many tissues, the thyroid and the liver included. Caffeic acid phenethyl ester (CAPE), a component of honeybee propolis, has been suggested to be also an effective antioxidant.
Agnieszka Kokoszko-Bilska; Jan Stępniak; Andrzej Lewiński; Malgorzata Karbownik-Lewinska. Protective antioxidative effects of caffeic acid phenethyl ester (CAPE) in the thyroid and the liver are similar to those caused by melatonin. Thyroid Research 2014, 7, 5 -5.
AMA StyleAgnieszka Kokoszko-Bilska, Jan Stępniak, Andrzej Lewiński, Malgorzata Karbownik-Lewinska. Protective antioxidative effects of caffeic acid phenethyl ester (CAPE) in the thyroid and the liver are similar to those caused by melatonin. Thyroid Research. 2014; 7 (1):5-5.
Chicago/Turabian StyleAgnieszka Kokoszko-Bilska; Jan Stępniak; Andrzej Lewiński; Malgorzata Karbownik-Lewinska. 2014. "Protective antioxidative effects of caffeic acid phenethyl ester (CAPE) in the thyroid and the liver are similar to those caused by melatonin." Thyroid Research 7, no. 1: 5-5.
Agnieszka Kokoszko-Bilska; Jan Stępniak; Andrzej Lewiński; Malgorzata Karbownik-Lewinska. Protective antioxidative effects of caffeic acid phenethyl ester in the thyroid and the liver are similar to those caused by melatonin. Endocrine Abstracts 2013, 1 .
AMA StyleAgnieszka Kokoszko-Bilska, Jan Stępniak, Andrzej Lewiński, Malgorzata Karbownik-Lewinska. Protective antioxidative effects of caffeic acid phenethyl ester in the thyroid and the liver are similar to those caused by melatonin. Endocrine Abstracts. 2013; ():1.
Chicago/Turabian StyleAgnieszka Kokoszko-Bilska; Jan Stępniak; Andrzej Lewiński; Malgorzata Karbownik-Lewinska. 2013. "Protective antioxidative effects of caffeic acid phenethyl ester in the thyroid and the liver are similar to those caused by melatonin." Endocrine Abstracts , no. : 1.
Fenton reaction (Fe(2+)+H(2)O(2) → Fe(3+)+()OH+OH(-)) is of special significance in the thyroid, as both substrates are indispensable for thyroid hormone synthesis, therefore being available presumably at high concentrations under physiological conditions. The study aimed at evaluation if both Fenton reaction substrates are required to induce oxidative damage to membrane lipids and nuclear DNA in porcine thyroid homogenates, and if these macromolecules are vulnerable to the same extent. Thyroid homogenates and nuclear DNA were incubated in the presence of H(2)O(2) and/or Fe(2+). Malondialdehyde+4-hydroxyalkenals (MDA+4-HDA) concentration (lipid peroxidation index) was measured spectrophotometrically, and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) concentration (DNA damage index) by HPLC. Whereas Fenton reaction substrates, used separately, did not affect lipid peroxidation, they increased 8-oxodG level for the highest H(2)O(2) concentration (100mM) and in Fe(2+) concentration-dependent manner (300, 150, 30 and 15 μM). If Fe(2+) and H(2)O(2) were applied together, lipid peroxidation increased significantly, however without H(2)O(2) concentration- but with clear Fe(2+) concentration-dependent effect. Concerning DNA damage, Fe(2+) enhanced H(2)O(2) effect, whereas Fe(2+) concentration-dependent effect was not changed by H(2)O(2). Excess of exclusively one of Fenton reaction substrates is sufficient to induce oxidative DNA damage, but not lipid peroxidation, in porcine thyroid. Comparing to H(2)O(2), Fe(2+) seems to be a stronger damaging substrate.
Jan Stępniak; Andrzej Lewiński; Malgorzata Karbownik-Lewinska. Membrane lipids and nuclear DNA are differently susceptive to Fenton reaction substrates in porcine thyroid. Toxicology in Vitro 2013, 27, 71 -78.
AMA StyleJan Stępniak, Andrzej Lewiński, Malgorzata Karbownik-Lewinska. Membrane lipids and nuclear DNA are differently susceptive to Fenton reaction substrates in porcine thyroid. Toxicology in Vitro. 2013; 27 (1):71-78.
Chicago/Turabian StyleJan Stępniak; Andrzej Lewiński; Malgorzata Karbownik-Lewinska. 2013. "Membrane lipids and nuclear DNA are differently susceptive to Fenton reaction substrates in porcine thyroid." Toxicology in Vitro 27, no. 1: 71-78.