Introduction: Cytokines are key mediators in immunological and inflammatory conditions, including chronic spontaneous urticaria (CSU). Objectives: To investigate Th1, Th2, and Th17 cytokine profiles in CSU and to evaluate the possible effect of omalizumab treatment. Methods: Patients who were followed up for CSU, as well as healthy volunteers, were included in the study. To assess urticaria activity, the 7-day-Urticaria Activity Score (UAS-7), the Urticaria Control Test (UCT), and the Chronic Urticaria Quality of Life Questionnaire (CU-QoL) were filled. Serum levels of IL-6, IL-17, IL-31, eotaxin, RANTES, TNF-α, and TSLP were analyzed by ELISA and compared in CSU and control groups. The patients were analyzed in two groups as the omalizumab group and the non-omalizumab group based on their treatment status. Results: Total IgE, ESR, CRP, RANTES, and TNF-a were significantly different in the overall comparison of the three groups: CSU-receiving omalizumab, CSU-not receiving omalizumab, and control groups (P <0.01, 0.015, <0.01, <0.01 and <0.01 respectively). Total IgE, CRP, RANTES, and TNF-α values were similar in those who received and did not receive omalizumab, yet these biomarkers were significantly higher in both groups than in the control group (P < 0.05). Statistical significance in ESR was observed only between the CSU-receiving omalizumab group and the control group (P = 0.01). Within the CSU patients, there was a slight but significant correlation between UCT and TNF-α (P = 0.008, r = 0.32) and IL-17 (P = 0.06, r = 0.33) levels. Conclusions: The investigated cytokine profile in CSU patients may differ from healthy controls, particularly with the higher levels of RANTES and TNF-α, and omalizumab treatment does not seem to affect that profile in CSU patients.

Nanocomposite hydrogels have potential in bone regeneration due to the inorganic and polymeric material content. In this study, new types of nanocomposite hydrogels composed of zoledronic acid/strontium hydroxyapatite nanoparticles and carboxymethyl chitosan/oxidized dextran (CMC/OD) hydrogels were reported. Pure hydroxyapatite, 5%, 10% and 15% (w/w) strontium-substituted strontium hydroxyapatite nanoparticles were produced and then modified with zoledronic acid at ratios of 5% to 7.5% (w/w). These modified structures were then incorporated into CMC/OD hydrogels. Zoledronic acid modified strontium hydroxyapatite nanoparticles were characterized using Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS) and X-ray Diffraction (XRD). CMC/OD structures were investigated using Fourier Transform Infrared Analysis (FTIR), Scanning Electron Microscopy (SEM). The physical properties of the hydrogels were determined via degradation behavior and rheological measurements. Cell–material interactions were investigated in vitro. The results showed that the incorporation of hydroxyapatite nanoparticles into CMC would significantly improve the rheological properties. The addition of strontium to hydroxyapatite nanoparticles significantly enhanced cell proliferation. In addition, a significant increase in alkaline phosphatase (ALP) and calcium deposition was observed with the addition of zoledronic acid. In conclusion, the nanocomposite hydrogels of CMC/OD containing zoledronic acid modified strontium hydroxyapatite demonstrate potential for orthopedic and craniofacial applications due to their superior properties, including the ability to be easily injected into targeted areas, potent antibacterial activity that helps prevent infections and remarkable self-healing capabilities that promote tissue regeneration and repair.

Chronic airway diseases represent a significant global health challenge, and reliable in vitro model systems are essential for elucidating the molecular mechanisms underlying these conditions. Although air–liquid interface (ALI) culture systems are among the most effective models for studying airway epithelial cells under physiological conditions, the nonbiodegradable membranes commonly used in current systems present certain limitations. In the present study, biodegradable poly­(l-lactic acid) (PLLA) and poly­(ε-caprolactone) (PCL) nanofiber membranes were fabricated using the electrospinning technique, and novel transwell membrane systems were developed. Optimization studies revealed that the nanofiber diameters ranged between 50 and 275 nm, forming a structure closely resembling the native lung extracellular matrix. Degradation analyses indicated that PLLA and PCL membranes remained structurally stable for up to six months, making them suitable for long-term in vitro airway modeling applications. Attenuated total reflectance–Fourier transform infrared (ATR-FTIR) spectroscopy confirmed the chemical stability of the membranes. Additionally, cell culture assays demonstrated high cell viability and strong cellular adhesion. Immunocytochemical analysis revealed β-tubulin expression in bronchial epithelial cells differentiated on the membranes, indicating successful epithelial maturation. These findings suggest that biodegradable membranes provide a promising platform for in vitro airway modeling. Furthermore, the use of biodegradable membranes is expected to address a critical need by accurately mimicking the tracheal and bronchial architecture as submucosal tissue analogues, thereby advancing the development of preclinical airway tissue graft constructs.