Neonicotinoid insecticides are ubiquitous in food and the environment due to their wide use. Growing evidence suggests the adverse effects of neonicotinoids in many species, including mammals. Some studies have reported the urinary concentrations of neonicotinoids in human biological monitoring, but the potential risks of neonicotinoids on human health based on long-term chronic exposure studies in any general population have been rarely tackled. In this study, the dietary exposure to neonicotinoids of the Chinese adult population was studied on the basis of composite dietary samples collected from the 5th (2009-2012) and 6th (2015-2018) Chinese total diet studies (TDS). Residue levels of ten neonicotinoids were determined in 528 composite dietary samples from 24 provinces in China. Most of the samples (53.3% and 70.5% in the 5th and 6th TDS, respectively) that we analyzed contained the multi-residue of neonicotinoids. Imidacloprid and acetamiprid were the most frequently detected neonicotinoids, and thiamethoxam and clothianidin were increasingly used and found in the 6th TDS. The estimated daily intake (EDI) for total neonicotinoids was calculated to evaluate health risk of the Chinese adult population based on a relative potency factor assessment method. The mean EDIs of total neonicotinoids in the 5th and 6th TDS respectively reached 598.95 and 710.38 ng/kg bw per day. Although the mean EDIs of total neonics in 6th TDS was relatively higher than that in 5th TDS, no statistical difference was observed (p > 0.05). Vegetables were the main source of dietary exposure, but exposure via cereals and beverages and water must also be addressed in China. Although the average exposure for total neonicotinoids was much lower than the current chronic reference dose (57 μg/kg bw per day), the dietary exposure risks of a general population for total neonicotinoids should not be overlooked due to the ubiquity of neonicotinoids in food and the environment.

Inhalation from ambient air and cigarette smoke is a common route of human exposure to polycyclic aromatic hydrocarbons (PAHs). Little information is available regarding hepatotoxicities of inhaled PAHs so for. In this study, we evaluated the toxic effects of intratracheally instilled benzo[a]pyrene (B[a]P) on hepatic lipid metabolism of C57BL/6 mice at relevant environmental exposure levels by using two different mass-based lipidomics approaches. The results of mass spectrometry imaging analysis showed that both the abundance and spatial distribution of several lysophosphatidylcholine (LysoPC), phosphatidylcholine (PC) and sphingomyelin (SM) in the liver section were different and changed after inhalation exposure to B[a]P. Liquid chromatography coupled with mass spectrometry-based lipidomics analysis and multivariate statistical analysis found that B[a]P exposure markedly altered glycerophospholipids, glycerolipids, and fatty acid metabolism in the mouse liver, with increasing of triacylglycerol (TG), phosphatidylinositol (PI) and PC, and decreasing of LysoPCs phosphatidylethanolamines (PEs), lysophosphatidylethanolamine (LysoPEs), free fatty acids (FFAs) and eicosanoids. B[a]P-induced lipid metabolic disorders showed a time-dependent effect, which generated three response trajectories with different change trends. Consequently, B[a]P exposure induced alteration of hepatic lipids by promoting the uptake from blood or the biosynthesis and transformation in the liver, might contribute to non-alcoholic fatty liver disease, hepatocyte membrane injury, inflammation, and signal system disturbance.