Background/Objectives: Inula helenium L. (Asteraceae) is widely used in traditional medicine, and its essential oil has demonstrated antimicrobial properties. The aim of this study was a comprehensive investigation of the chemical composition and biological activity of a concentrated extract of Inula helenium L. roots, including the determination of the main groups of biologically active substances and the assessment of antioxidant, antimicrobial, anti-aggregatory and anticoagulant properties. Methods: Inula helenium L. roots were extracted by UAE, the optimal conditions were double extraction with 70% ethanol at 30 kHz for 30 min. Chemical profiling was performed by HPLC-ESI-MS against 20 reference phenolic compounds, with method validation. Antimicrobial activity was assessed by microdilution against Gram-positive, Gram-negative and fungal strains (MBC/MFC). Antioxidant activity was evaluated in two chemiluminescence model systems simulating reactive oxygen species generation and lipid peroxidation. Anti-aggregatory and anticoagulant effects were studied in vitro on platelet-rich and platelet-poor plasma. Results: 11 polyphenolic compounds were identified and quantified, including five phenolic acids (gallic, chlorogenic, caffeic, ferulic and p-coumaric acids) and six flavonoids (hyperoside, naringenin, quercetin, kaempferol, luteolin and apigenin). The extract exhibited antimicrobial activity with MBC values of 3.91–62.5 μg/mL against Gram-positive bacteria, 2.5–10 μg/mL against Gram-negative bacteria, and 10 μg/mL against Candida albicans. The extract moderately inhibited platelet aggregation, prolonged aPTT, and reduced chemiluminescence by 15–20% relative to the control. Conclusions: The thick extract of Inula helenium L. roots contains bioactive phenolic compounds and exhibits moderate antimicrobial, antioxidant and anticoagulant activity, supporting its potential as a natural multifunctional agent and justifying further in vivo studies and dosage form development.

This study investigated manufacturing conditions for suppositories containing a CO₂ extract of Rosmarinus officinalis L. herb, using a previously developed formulation, and evaluated a gas chromatography procedure with flame ionization detection (GC–FID) for quantitative marker determination. The effects of key manufacturing parameters were evaluated, and the optimal conditions for base preparation were determined as 70 °C and 120 rpm for 12 min; increasing the agitation speed to 150 rpm did not reduce the processing time. Extract concentrate preparation at 37 °C and 60 rpm required 25 min to achieve visually assessed homogeneity. At 60 rpm, prior concentrate preparation reduced the subsequent mixing time of the suppository mass from 100 to 20 min at each investigated temperature (37, 45, and 60 °C), excluding the time required to prepare the concentrate. Cooling at 8 °C for 60 min was selected on the basis of mechanical-strength measurements. Agar diffusion experiments provided a comparative assessment of the diffusion of colored extract constituents rather than a quantitative measure of chamazulene release or bioavailability. The findings identify candidate manufacturing conditions for further evaluation. Confirmation of marker identity, analytical validity, quantitative content uniformity, and long-term stability is required before reliable product standardization can be established.