Indigenous and local knowledge systems are characterised by a ‘knowledge-practice-belief’ complex that plays a critical role for biodiversity management and conservation on indigenous lands. However, few studies take into consideration the interconnected relationship between the social processes underpinning knowledge accumulation, generation and transmission. The study draws on ethnobotanical research to explore plant uses, practices and belief systems developed among the indigenous Vhavenda in South Africa for sustaining indigenous plant resources and highlights some of the forces of change influencing the acquisition and transmission of knowledge. Data was collected from September–November 2016 from 31 individuals by means of semi-structured interviews; walks in home gardens, cultivated fields, montane forests and deciduous woodlands; and vouchering of plant species in six villages (Duthuni, Tshidzivhe, Vuvha, Lwamondo, Mashau and Tshiendeulu) in the Vhembe District of South Africa. The Use Value Index (UVI) was used to measure the number of different uses of each species and the Relative Frequency Index (RFI) to measure the local importance of each species. Semi-structured interviews and comparisons with published works also explored cultural practices and belief systems associated with plants, modes and barriers of knowledge transmission. Eighty-four plant species were reported within 44 families, with Fabaceae representing the highest diversity of plant species. We identified six species not previously documented in the Vhavenda ethnobotanical literature, 68 novel uses of plants and another 14 variations of known uses. Vhavenda plants were predominantly used for food (36.0%) and medicine (26.1%) and consisted mainly of native (73.8%) compared to non-native species (26.2%). The Vhavenda possess a range of practices for managing plant resources that can be attributed to taboos preventing the use of selected species, promotion of sustainable harvesting practices and the propagation of plant species for ecological restoration. Plant knowledge and management practices were transmitted from relatives (48.4%), self-taught through time spent planting and harvesting plants on the land (19.4%), through apprenticeships with traditional healers (16.1%), initiation schools (9.7%) and clan gatherings (6.4%). Changes in traditional learning platforms for knowledge exchange, erosion of cultural institutions and shifting value systems serve as barriers for knowledge transmission among the Vhavenda. The study points to a need for new partnerships to be forged between conservationists, government actors and local and indigenous knowledge holders to foster hybrid knowledge coproduction for developing strategies to enhance the productivity and biodiversity of indigenous lands.

The aim of this study was to determine the antioxidant activity of the extractable and non-extractable phenolics of Terminalia. Sericea Burch. Ex DC. Free, ester bound, ether or glycoside bound and insoluble phenolics were extracted from the fruit, leaves, stem, and root samples. Follin Ciocalteu was used to estimate the phenolic content while DPPH (2,2-diphenyl-1-picrylhydrazyl) assay was used to determine the antioxidant activity. The data obtained were subjected to multivariate analysis for relationships. The result indicated that the highest average total phenolic contents and antioxidant activities were found in the free (14.8 mgGAE/g; IC₅₀ 6.8 μg/mL) and ester bound (15.1 mgGAE/g; IC₅₀ 6.4 μg/mL) extractable phenolics. There was a strong negative correlation between TPC and DPPH (r = −0.828). Agglomerative hierarchical clustering revealed three clusters. Cluster one contained the insoluble and glycoside phenolics while cluster 2 contained only free phenolic acid of the root. The third cluster was predominantly free and ester bound phenolic extracts. The principal component analysis score plot indicated two major clusters with factor 1 (F1) explaining 61% of the variation. The nuclear magnetic resonance spectroscopy spectra indicated that gallic acid and resveratrol are the major phenolic compounds present in the root. This study has demonstrated that extractable phenolics contributed more to the antioxidant activities compared to the non-extractables.

Malaria parasites are increasingly becoming resistant to currently used antimalarial therapies, therefore there is an urgent need to expand the arsenal of alternative antimalarial drugs. In addition, it is also important to identify novel antimalarial drug targets. In the current study, extracts of two plants, Pterocarpus angolensis and Ziziphus mucronata were obtained and their antimalarial functions were investigated. Furthermore, we explored the capability of the extracts to inhibit Plasmodium falciparum heat shock protein 70 (Hsp70) function. Heat shock protein 70 (Hsp70) are molecular chaperones whose function is to facilitate protein folding. Plasmodium falciparum the main agent of malaria, expresses two cytosol-localized Hsp70s: PfHsp70-1 and PfHsp70-z. The PfHsp70-z has been reported to be essential for parasite survival, while inhibition of PfHsp70-1 function leads to parasite death. Hence both PfHsp70-1 and PfHsp70-z are potential antimalarial drug targets. Extracts of P. angolensis and Z. mucronata inhibited the basal ATPase and chaperone functions of the two parasite Hsp70s. Furthermore, fractions of P. angolensis and Z. mucronata inhibited P. falciparum 3D7 parasite growth in vitro. The extracts obtained in the current study exhibited antiplasmodial activity as they killed P. falciparum parasites maintained in vitro. In addition, the findings further suggest that some of the compounds in P. angolensis and Z. mucronata may target parasite Hsp70 function.

Over a long period of time, cycads endemic to Africa have been facing high risk of extinction. Several conservation efforts have been made to reduce the risk of losing these highly endangered species. In this study we review the current risk of extinction of all African cycads species. We calculated the percentages of each category of species found in African cycads using the IUCN (International Union of Conservation of Nature) red list of threatened species 2014 version. We compared our result with that of Donaldson (2003) on percentages of different categories of IUCN for cycads in Africa which was carried out a decade ago. We also calculated the percentage of population trend in African cycads. When comparing these results, we discovered that over one decade there was no improvement in cycads conservation despite several conservation efforts that were made during this period. The results of the population trend also showed that the majority of African cycads are experiencing population decreases. These results highlight that the risk of extinction of African cycads is still very high and much conservation effort is still required to properly tackle ecological factors pushing these endangered species to extinction.

There is an established link between deforestation and negative hydrological effects which may affect watersheds. The number of the cycads Encephalartos transvenosus Stapf & Burtt Davy in South Africa is drastically reducing, and they are nearly extinct. Additionally, poaching and reduction in the population of cycads in reserves may impact negatively on watersheds in protected and buffer areas since the regeneration of this plant occurs over a long period. This research aimed to study the distributions of poached cycads, evaluate the factors responsible for poaching activity and suggest possible solutions towards conservation of E. transvenosus Stapf & Burtt Davy in some of the nature reserves in Limpopo province. Field observation was used to ascertain and collect locations of poached cycads. Data was obtained through the use of a questionnaire. Questionnaires were administered to rangers for the collection of data on causes of poaching, parts poached, measures taken and challenges of rangers in the nature reserves. Processing of the collected data was done by simple statistical analysis. The total population of rangers available in the reserves was used for the determination of the sample size of rangers by Slovin’s formula. Unemployment (46%) is the leading cause for poaching in Mphaphuli Nature Reserve, while trade is a leading factor in Modjadji and Lekgalameetse (37% and 60%) respectively. Debarked cycads amount to 14 (54%) while 12 of the total (26 poached cycads) were completely removed (46%). The most effective way of stopping the poaching of cycads as perceived by the rangers is by patrolling the nature reserves. The observed poaching of Encephalartos transvenosus is alarming since this plant has a low population size which is currently confined to the Limpopo Province in South Africa only. If poaching continues in the nature reserves, all cycads have the tendency of being removed. Therefore, there is a need to continually develop strategies for the conservation of cycads. The peculiar nature and challenges of each reserve have been established and unique solutions for these nature reserves in Limpopo Province have been proposed. Better conservation of cycad species can positively impact the hydrological process of the concerned area and better help the watersheds.

Victor PM, Tshisikhawe MP, Magwede K. 2023. Population dynamics of Spirostachys africana Sond. a species highly exploited amongst some communities of Limpopo Province, South Africa. Biodiversitas 24: 492-497. Over-exploitation of important indigenous plant species impact negatively on their population structure. Unmonitored and unsustainable harvesting from plant ecosystems may inhibit plant recovery. The aim of the study was to investigate the population structure of Spirostachys africana in a communal area of Ha-Matsa Village in Limpopo Province, South Africa. Eleven transects of 100m x 10m were used to sample ecological parameter data from 66 plant specimens of S. africana species. Transects were subjectively placed within the population of S. africana. Data on plant height size classes and basal stem diameter size classes exhibited an almost Bell-shaped population curve. The population of S. africana did not have many seedlings, with the Crown health status data revealing a population with generally moderately damaged crowns (24%), while 19% of individuals had healthy crowns. The concern is the recorded 9% of dead crowns. Although the population looks great from a distance, the sampled ecological data revealed an unviable population. The Spirostachys africana population of Ha-Matsa Village does not have more individuals in the small size classes of both height and basal stem diameter. Conservation practice implies that if anthropogenic activities are not properly monitored, there may not be enough young individuals to support the population of S. africana into the future. Therefore, this study aimed to improve the assessment and the effects of harvesting on the S. africana species population. This study also suggests important information and guides ecologists and conservationists on sustainable harvesting practices and offtakes of S. africana.