Characterizing the viscoelastic properties of thin-layer tissues with micro-level thickness has long remained challenging. Recently, several micro-elastography techniques have been developed to improve the spatial resolution. However, most of these techniques have not considered the medium boundary conditions when evaluating the viscoelastic properties of thin-layer tissues such as arteries and corneas; this might lead to estimation bias or errors. This paper aims to integrate the Lamb wave model with our previously developed ultrasonic micro-elastography imaging system for obtaining accurate viscoelastic properties in thin-layer tissues. A 4.5-MHz ring transducer was used to generate an acoustic radiation force for inducing tissue displacements to produce guided wave, and the wave propagation was detected using a confocally aligned 40-MHz needle transducer. The phase velocity and attenuation were obtained from k-space by both the impulse and the harmonic methods. The measured phase velocity was fit using the Lamb wave model with the Kelvin-Voigt model. Phantom experiments were conducted using 7% and 12% gelatin and 1.5% agar phantoms with different thicknesses (2, 3, and 4 mm). Biological experiments were performed on porcine cornea and rabbit carotid artery ex vivo . Thin-layer phantoms with different thicknesses were confirmed to have the same elasticity; this was consistent with the estimates of bulk phantoms from mechanical tests and the shear wave rheological model. The trend of the measured attenuations was also confirmed with the viscosity results obtained using the Lamb wave model. Through the impulse and harmonic methods, the shear viscoelasticity values were estimated to be 8.2 kPa for 0.9 Pa·s and 9.6 kPa for 0.8 Pa·s in the cornea and 27.9 kPa for 0.1 Pa·s and 26.5 kPa for 0.1 Pa·s in the artery.

Objective: to both qualitatively and quantitatively investigate corneal biomechanical properties through an ultrasonic micro-elastography imaging system, which is potentially useful in diagnosis of diseases such as keratoconus, post-refractive keratectasia and tracking treatment such as cross-linking surgery. Methods: our imaging system has a dual frequency configuration, including a 4.5 MHz ring transducer to push the tissue and a confocally aligned 40 MHz needle transducer to track micron-level displacement. 2D/3D acoustic radiation force impulse (ARFI) imaging and the Young's modulus in the region of interest were performed on ex vivo porcine corneas that were either cross-linked using formalin solution or preloaded with IOPs from 5 mmHg to 30 mmHg. Results: the increase of corneal stiffness and the change in cross-linked volume following formalin crosslinking could be precisely observed in the ARFI images and reflected by the reconstructed Young's modulus while the B-mode structural images remained almost unchanged. In addition, the relationship between the stiffness of the cornea and IOPs was investigated among twelve porcine corneas. The corneal stiffness is significantly different at various IOPs and has a tendency to become stiffer with increasing IOP. Conclusion: Our results demonstrate the principle of using ultrasonic micro-elastography techniques to image the biomechanical properties of the cornea. Integrating high-resolution ARFI imaging labelled with reconstructed Young's modulus and structural imaging of the cornea can potentially lead to a routinely performed imaging modality in the field of ophthalmology.

Estimating the corneal elasticity can provide valuable information for corneal pathologies and treatments. Ophthalmologic pathologies will invariably cause changes to the elasticity of the cornea. For example, keratoconus and the phototoxic effects of ultraviolet radiation usually increase the corneal elasticity. This makes a quantitative estimation of the elasticity of the human cornea important for ophthalmic diagnoses. The present study investigated the use of a proposed high-resolution shear wave imaging (HR-SWI) method based on a dual-element transducer (comprising an 8-MHz element for pushing and a 32-MHz element for imaging) for measuring the group shear wave velocity (GSWV) of the human cornea. An empirical Young’s modulus formula was used to accurately convert the GSWV to Young’s modulus. Four quantitative parameters, bias, resolution, contrast, and contrast-to-noise ratio (CNR), were measured in gelatin phantoms with two different concentrations (3% and 7%) to evaluate the performance of HR-SWI. The biases of gelatin phantoms (3% and 7%) were 5.88% and 0.78%, respectively. The contrast and CNR were 0.76, 1.31 and 3.22, 2.43 for the two-side and two-layer phantoms, respectively. The measured image resolutions of HR-SWI in the lateral and axial directions were 72 and 140 μm, respectively. The calculated phase SWV (PSWV) and their corresponding Young’s modulus from six human donors were 2.45 ± 0.48 m/s (1600 Hz) and 11.52 ± 7.81 kPa, respectively. All the experimental results validated the concept of HR-SWI and its ability for measuring the human corneal elasticity.

Objective: Estimating the elasticity distribution in the cornea is important because corneal elasticity is usually influenced by corneal pathologies and surgical treatments, especially for early corneal sclerosis. Because the thickness of the cornea is typically less than 1 mm, high-resolution ultrasound elastography as well as the Lamb wave model is required for viscoelastic property estimation. In the present study, an array high-frequency ultrasound (HFUS) elastography method based on ultrafast ultrasound imaging was proposed for estimating the viscoelastic properties of porcine cornea. Methods: The elastic wave was generated by an external vibrator, after which the wave propagation image was obtained using a 40-MHz array transducer. Viscoelasticity estimation was performed by fitting the phase velocity curve using the Lamb wave model. The performance of the proposed HFUS elastography system was verified using 2-mm-thick thin-layer gelatin phantoms with gelatin concentrations of 7% and 12%. Ex vivo experiments were carried out using fresh porcine cornea with artificial sclerosing. Results: Experimental results showed that the estimated elasticity was close to the standard value obtained in the phantom study when the Lamb wave model was used for elasticity measurement. However, the error between the standard elasticity values and the elasticity values estimated using group shear wave velocity was large. In the ex vivo eyeball experiments, the estimated elasticities and viscosities were respectively 9.1 ± 1.3 kPa and 0.5 ± 0.10 Pas for a healthy cornea and respectively 15.9 ± 2.1 kPa and 1.1 ± 0.12 Pas for a cornea with artificial sclerosis. A 3D HFUS elastography was also obtained for distinguishing the region of sclerosis in the cornea. Conclusion The experimental results demonstrated that the proposed HFUS elastography method has high potential for the clinical diagnosis of corneal diseases compared with other HFUS single-element transducer elastography systems.

Tendon stiffness plays an important role in the tendon healing process, and many studies have indicated that measuring the shear wave velocity (SWV) on tendons relates to their stiffness. Because the thickness of hand tendons is a few millimeters, high-resolution imaging is required for visualizing hand tissues. However, the resolution of current ultrasound elastography systems is insufficient. In this study, a high-frequency (HF) ultrasound elastography system is proposed for measuring the SWVs of hand tendons. The HF ultrasound elastography system uses an external vibrator to create shear waves on hand tendons. Then, it uses a 40-MHz HF ultrasound array transducer with ultrafast ultrasound imaging technology to measure the SWV for characterizing hand tendons. A handheld device that combines a transducer and a vibrator allows the user to scan hand tissues. The biases of HF ultrasound elastography were measured in gelatin phantom experiments and were less than 6% compared to standard mechanical testing approach. Human experiments showed the ability to use HF ultrasound elastography to distinguish different SWVs of hand tendons. The SWVs were 0.73 ± 0.65 m/s and 1 ± 0.54 m/s for flexor digitorum superficialis (FDS) and flexor digitorum profundus (FDP), respectively, and 0.52 ± 0.14 m/s and 4.02 ± 0.77 m/s for extensor tendon under stretch and contraction conditions, respectively. The simplicity and convenience of the HF ultrasound elastography system for measuring hand tendon stiffness make it a promising tool for evaluating the severity of hand injuries and the performance of rehabilitation after hand injuries.