Correct management of the feeding regime in shrimp aquaculture has been beneficial. Still, when looking for improvement in shrimp performance, the results have been contradictory, and the limits between better growth and independent growth as a function of the feeding regime are not clear. In this study, trypsin and α-amylase activity, as well as an interpretation of the energy utilized for enzyme production, were evaluated in shrimp weighing 1 g. Four feeding groups were set to feed one, two, four, or eight times per day over a month, after which trypsin and α-amylase activities were evaluated during 29 h. Results indicated that the group fed once per day ingested 90% of the feed, whereas the other groups ingested 100%. The α-amylase was not consumed during the daytime in all groups, unlike trypsin. Total trypsin activity was not significantly different between feeding groups, but α-amylase was significantly different. Shrimp fed eight times had an elevated α-amylase activity level that was 2.6 times greater than those fed only once, and 0.8 and 0.5 times greater than those fed four and twice per day, respectively. Feeding more frequently generates a higher use of energy that may or may not be reflected in growth but could be essential for all the energy-dependent metabolic processes required by shrimp.

Aquaculture wastewater supplies nutrients for seaweed cultivation, but its suitability for producing functional polysaccharides remains uncertain. This critical review examines the evidence connecting wastewater composition, nutrient assimilation, polysaccharide production, macromolecular architecture, and agricultural performance. Studies of wastewater-grown seaweeds were compared with controlled cultivation, extraction, and agricultural application studies, with attention to nitrogen and phosphorus availability, C:N balance, salinity, and dissolved inorganic carbon. Evidence indicates that nutrient enrichment can improve biomass production without corresponding increases in polysaccharide yield. Measurements linking wastewater chemistry to molecular weight distribution, monosaccharide composition, sulfation, and polymer conformation remain scarce. Differences among species, growth conditions, and nutrient exposure regimes complicate comparisons across studies. Extraction and purification can alter polymer characteristics, limiting the attribution of observed differences to cultivation conditions. Reported agricultural responses may depend on polymer structure and formulation. However, the independent effects of molecular weight and other structural descriptors remain poorly established experimentally. Contaminant transfer from wastewater to purified products and subsequent exposure of soil and crops are inadequately characterized. Future research should combine matched wastewater and conventional cultivation controls, standardized extraction, polymer characterization, contaminant mass balances, and dose–response bioassays. Integrated economic and environmental assessment is also required before product safety, functional equivalence, and practical feasibility can be established.