Intra-articular injection of platelet-rich plasma offers a promising potential for treatment of osteoarthritis in dogs. Twenty dogs weighing 25 to 50 kg (mean 38 kg) with unilateral stifle osteoarthritis were used for the study. Fourteen dogs were given intra-articular platelet rich plasma treatment and 6 dogs were used as controls. Double centrifuge method was used to obtain platelet-rich plasma. Radiography and ultrasonography of the affected joint were carried out and scores for lameness severity and pain severity were assigned by the attending clinicians. Synovial fluid was collected under sterile conditions at pre-treatment and on the 1 ^st , 3 ^rd , 5 ^th , 7 ^th , 15 ^th days, and 4, 8 and 12 weeks after treatment. Gelatin zymography and Enzyme-linked Immunosorbent Assay were used to determine the synovial fluid levels of matrix metalloproteinase-2 (MMP-2) and metalloproteinase-9 (MMP-9). No adverse effects associated with the injection of the platelet concentrate or saline were observed. Hudson Visual Analog Scale and Canine Brief Pain Inventory scores for all components were non-significantly different between weeks 0, 4, 12 for control dogs. Matrix metalloproteinase-9 was totally and MMP-2 was partially inhibited in the platelet-rich plasma group. In the control group, MMP-9 was partially inhibited during the first month and activation started later. Matrix metalloproteinase-2 was constant in control samples throughout the experimental period. Platelet-rich plasma is a safe and effective method for treatment of dogs with osteoarthritis, possibly more useful for early cases with mild and moderate osteoarthritis. It is suggested that plasma rich platelet should be injected several times at regular intervals instead of a single application.

In this study, the effects of the wound‐covering materials, Acticoat^® and Cutinova Hydro^®, on wound healing have been studied in rabbit models with open and tissue‐lost wounds with full‐thickness flank excisions. Rabbits were used as subjects with three groups of four rabbits each, and trial periods of 7, 14 and 21{\uns}days. Four circular wounds, of 1.5 cm diameter were made two on the right (one of them control) and two on the left (one of them control) of the dorsal sides of the abdomen. Acticoat^® and Cutinova Hydro^® were applied on the wounds with suture for a period of 21 days and one each placed on the right and left sides as control with gauze. Biopsy specimens were taken from the animals at the end of the research period to check the length of the epithelium, epithelial thickness, size of wounds, wound granulation tissue formation and histopathological evaluation for clarity. The Acticoat^® group showed better healing and scar formation compared to the Cutinova Hydro^® group by macroscopic examination. Epithelial wound length and clarity in terms of statistical difference occurred on day 21 (P 0.05). As a result, the Acticoat^® wound dressing was determined as a more reliable for the early wound healing. This study has shown the short‐term clinical benefits of hydroactive, polyurethane dressings in the management of acute wounds. However, longer periods of wound healing procedure should be planned for reliable and safe results of wound dressing. It has also been concluded that microbiological analyses should be included for more robust and reliable comparisons.

The objective of this study intended the application of the current technique of modified tibial tuberosity advancement (TTA)-rapid method in dogs with cranial cruciate ligament rupture (CrCL) and reports on the clinical outcome and complications. The study material consisted of 17 male dogs of different breeds between the ages of 2-8 years, average weight of 32 kg with a diagnosed unilateral CCL rupture. Hudson Visual Analog Scale (HVAS)-Walking Test Rating, Canine Brief Pain Inventory (CPBI)-Pain Rating) were examined, and rupture of the CCL was diagnosed based on direct radiographic examination. The pre-operative evaluation of the implant to be used in TTA-Rapid technique was determined by measuring radiographic images of the CCL in dogs. Post-operative evaluation in the 1st, 2nd, and at 3 month was performed by radiographic examination, gait and pain tests. Major complications were recorded in 17.6% of the cases. Fourteen (82.4%) dogs had a good to excellent outcome 3 months after surgery. Lameness and pain assessment were performed up to 15 days prior to the operation and 1, 2, and 3 months after the TTA-Rapid technique procedure. It can be concluded that TTA-Rapid technique procedure is a fast, easy to learn and non-invasive treatment of CrCL ruptures in dogs.

Background: Lameness results in major economic losses on dairy farms. The proportion of lameness in cows are between 4-55%, the origin of 90% lameness are caused by foot diseases. Hoof abnormalities such as laminitis are major contributors to lameness. Aim of the study, compared with thermographic imaging (IRT) and radiographic, magnetic resonans (MR), computer tomography (CT), histopathological analysis on the diagnosis of laminitis.Materials, Methods & Results: Preliminary, the animals (20 Holstein-Fresian) were selected by lameness examination for evaluation of the for any obvious signs of disease. The animals were examined when rising and walking of the free stalls. Walking was very painful and clinical symptoms of general distress become present for laminitic group. The cows were scored on a 1 to 5 scale for their locomotion (1 = normal locomotion; 5 = severely lame). Thermographic examinations were performed the all animals after the routine clinical examination methods. The infrared images were taken from the dorsal view of all hoovesto monitor the temperature of coronary band. All images were scanned using a hand-held portable infrared camera (Wahl, Thermal Imager HSI3000 Series), which was calibrated to ambient temperature and absorptive conditions. Incentives radiological examinations, dorso-palmar and latero-medial shooting was done for using of (Regius Model 110 Konica, Minolta) 70 kV and 30 mAs for each claws. MRI images of all isolated digits were performed at the Department of Radiology, by using Siemens Symphony Erlangen, Germany. Continuous series of transversal, sagital and dorsoplantar scan were obtained from all digit. Computer tomography (CT), isolated digits were performed at the Imaging Department of Radiology, by using Siemens Symphony Erlangen, Germany. CT scanner was used to obtain contiguous slices from the region of the proximal sesamoid bones to the toe. Anterior side (laminar and perilaminar tissues) of the claws was uncovered with electric wheel. Tissue samples were taken 10% buffered formaldehyde solution after establishing a routine laboratory procedure following the prepared paraffin blocks 5 micrometers thick sections with hematoxylineosin dye method according to the staining. It was finally examined by light microscopy. The computer soft ware of the infrared thermal camera showed increased local temperature, by 0.5-1.5°C, between the normal and suspected regions for laminitic cases. Clinically, animals suspected of laminitis were showed general stiffness, lameness during walking difficulty. Examination of the claws was demonstrated of rings and plump appearance was evident in paries ungulae. The main damage was seen during trimming of the sole from no hemorrhages or discoloration of control group. The transversal MRI images provided excellent depictions of anatomical structures when compared to their corresponding pictures. Identifiable anatomic structure was labeled on the line drawings of the limb sections and on the corresponding CT images. Twenty out of 30 claws (65%) were shown laminitis statistical with histopathological examination. In histopathological examination; mononuclear cells and neutrophil granulocytes infilrations and edema, haemorrhagia, mild hyperemia in the lamellar region was remarkable in laminitic cases.Discussion: As a results, thermographic examination may have potential as a detection tool for laminitis. MR transversal images provided excellent depiction of anatomical structures and many biometric research in the bovine hoof can be easily investigated. The usefulness of IRT, MRI, CT in evaluating laminitis in the acute patient remains stil open. The use of diagnostic techniques during the initial active phase of laminitis as a means to increase the understanding of the disease and also serves as a justification for the development of an experiment involving live cows induces laminitis.

This study aim to evaluate the modified Glasgow Coma Scale (mGCS) and Animal Trauma Triage (ATT) scores, labo- ratory variables, and prognostic features of trauma-induced endothelial glycocalyx injury in cats with head trauma. Twenty-five cats with head trauma and 10 healthy cats were evaluated in this study. The enzyme-linked immunosorbent assay (ELISA) method was used to measure the levels of syndecan-1 and thrombomodulin in the serum of the 25 cats with head trauma (within 48 hours) and the 10 healthy cats. In addition, mGCS scores, ATT scores, laboratory values, syndecan-1 and thrombomodulin levels were compared between the cats that survived following treatment and the cats that did not survive despite treatment. Syndecan-1 and thrombomodulin levels were not statistically different between healthy cats and cats with head trauma. In the cats with head trauma, the mGCS scoring system was found to be more sensitive than the ATT scoring system. In conclusion, syndecan-1 and thrombomodulin levels did not yield statistically significant results in the cats with head trauma. VREDNOTENJE SISTEMA OCENJEVANJA TRAVME IN POŠKODB ENDOTELIJSKEGA GLIKOKALIKSA PRI MAČKAH S POŠKODBO GLAVE Izvleček: Namen te študije je bil ovrednotiti spremenjeno glasgowsko lestvico kome (angl. Glasgow Coma Scale, GCS) in ocene ATT (Animal Trauma Triage), laboratorijske spremenljivke in prognostične značilnosti s travmo povzročene poškodbe endotelijskega glikokaliksa pri mačkah s poškodbo glave. V študijo je bilo vključenih 25 mačk s poškodbo glave in 10 zdravih mačk. Ravni sindekana-1 in trombomodulina v serumu 25 mačk s poškodbo glave (v 48 urah) in 10 zdravih mačk smo merili z encimsko imunoadsorpcijsko preiskavo (ELISA). Ocene mGCS, ocene ATT, laboratorijske vrednosti ter ravni sindekana-1 in trombomodulina smo primerjali med mačkami, ki so po zdravljenju preživele, in mačkami, ki kljub zdravljenju niso preživele. Vrednosti sindekana-1 in trombomodulina se med zdravimi mačkami in mačkami s poškodbo glave niso statistično razlikova- le. Pri mačkah s poškodbo glave se je sistem točkovanja mCGS izkazal za občutljivejšega od sistema točkovanja ATT. Zaklju- čili smo, da vrednosti sindekana-1 in trombomodulina pri mačkah s poškodbo glave niso statistično pomembne. Ključne besede: triaža poškodb živali; mačka; poškodba glave; spremenjena glasgowska lestvica kome; endotelijski glikokaliks