This study compared and analyzed changes in the microclimate and thermal environment inside single-span greenhouses covered with a single layer of plastic film, polycarbonate (PC), and glass. The results of the experiment show that the PC-covered greenhouse was the most favorable for managing the nighttime heating effect during the cold season. However, the glass-covered greenhouse was found to be the most favorable for managing the cooling effect during the hot season. Although the plastic-covered greenhouse was inexpensive and easy to install, the air temperature inside varied significantly, and it was difficult to control its indoor environment. The thermal load leveling values showed that the PC-covered greenhouse had the lowest variation, confirming its superiority in terms of environmental control and energy savings. In terms of the overall heat transfer, heat was generally transferred from the interior to the exterior of the greenhouses. In the plastic-covered greenhouse, however, heat was transferred in the opposite direction at night due to the influence of radiant cooling. The occurrence of the minimum and maximum heat transfer values had a tendency similar to that of the occurrence of the minimum and maximum air temperatures inside the greenhouses.