Microelectrode arrays play an important role in prosthetic implants for neural signal recording or applying electrical pulses stimulation to target nerve system. Safety and long-term reliability are essential requirements for microelectrode arrays applied in electrical stimulation. In design and fabrication of the microelectrode array, soft materials are generally chosen to be the substrate for the aim of achieving better compliance with the surrounding tissue while maintaining minimal damage. By flexing of the array to the surface, the array is capable of keeping a more stable electrical contact resulting in a significantly improved signal detected. In this study, we design and fabricate a flexible microelectrode array with gold as the electrode material and parylene-C as the substrate. The fabrication process of the array is presented. The in vitro electrochemical characteristics of the microelectrode are investigated by electrochemical impedance spectroscopy and cyclic voltammetry in a three-electrode electrochemical cell containing phosphate-buffered saline. Charge injection capacity measurements are carried out by multichannel systems and the CSC of the microarray is calculated. Electrochemical results showed that impedance decreased with frequency. The average impedance of the Au electrodes at 1 kHz was 36.54 ± 0.88 kΩ. The average phase angle at 1 kHz was − 73.52 ± 1.3°, and the CIC of the microelectrode was 22.3 µC/cm2. The results demonstrated that the microelectrode array performed as expected for neuronal signal recording or stimulation. With parylene-C as the substrate, the microarray has good flexibility. The electrochemical characteristics’ results show that the array has the ability to resist any corrosion on metal–electrolyte interface and has good biocompatibility. This low-cost, flexible parylene-based, gold microelectrode array shows potential for use in implant neurological signal acquisition or neurostimulation applications.

Purpose: P300 component of event related potentials in response to visual and auditory stimulation has been widely used in brain–computer interfaces (BCI). In clinical applications, tactile stimulus based on somatosensory electrical stimulation is an alternative for patients with impaired vision or hearing. This study presents an online P300 BCI based on somatosensory electrical stimulation paradigm. P300 signals were elicited by tactile selective attention of electrical stimuli on four fingers. Methods: Fifteen healthy subjects participated in this study. Participants’ task was to focus their attention on the target finger and count the number. The classification of P300 signals was performed by step-wise linear discriminate analysis. Results: The average classification accuracy of the somatosensory BCI was 79.81 ± 7.91%, with the information transfer rate at 4.9 ± 1.3 bits/min. The BCI performance on different time windows was also evaluated in the present study. Conclusions: Our results demonstrate the feasibility of employing somatosensory electrical stimuli to build a practical online P300 BCI without taxing the visual and auditory channel, providing a wider application prospect in clinical applications and daily life. We anticipate our diagram to be a starting point for more explorations on utilizing electrical somatosensory stimuli in conjunction with portable BCI for neural rehabilitation.

Early detection of neural conductivity changes at the compressed spinal cord is important for predicting the surgical outcomes of patients with cervical spondylotic myelopathy (CSM). The prognostic value of median nerve somatosensory evoked potential (SEP) has been proposed previously. The present prospective study evaluates the use of trial-to-trial variability in SEP as a valuable predictor of neurological recovery after surgery of CSM. A total of 35 CSM patients who underwent surgery with up to 6-month follow-up were recruited in this study. SEP signals were recorded preoperatively. The single trial SEP was extracted by a newly developed second-order blind identification method. The postoperative recovery was assessed using the modified Japanese Orthopaedic Association. The correlation between the latency variability of trial-to-trial SEP and post-operative recovery ratio was analyzed. The prognostic value of trial-to-trial SEP for CSM was evaluated using a receiver operator characteristic curve which can accurately reflect the relationship between sensitivity and specificity of a diagnostic method and represent the accuracy of prognosis. The correlation coefficient of trial-to-trial latency variability and the 6-month recovery ratio was statistically significant (r = −0.82, P < 0.01). The trial-to-trial SEP had a higher prognostic accuracy (AUC = 0.928, P < 0.001) with an optimal prognostic value of 9.25 % compared with averaged SEP when the threshold of recovery ratio was 40 %, and was more sensitive (93.80 %) than the averaged SEP (43.80 %). These findings indicate that the latency variability of trial-to-trial SEP reflect the recovery ratio of CSM patients after surgery. It is suggested that the latency variability of trial-to-trial SEP is useful for predicting the surgical outcomes for patients with CSM, which would be a potential indication of surgical treatment for CSM to help decision making of surgical planning for CSM patients.