The aim of this study was to assess the effect of elevated temperature on the growth, morphology and spatial orientation of lupine roots at the initial stages of development and on the formation of lupine root architecture at later stages. Two lupine species were studied—the invasive Lupinus polyphyllus Lindl. and the non-invasive L. luteus L. The plants were grown in climate chambers under 25 °C and simulated warming at 30 °C conditions. The angle of root curvature towards the vector of gravity was measured at the 48th hour of growth, and during a 4-h period after 90° reorientation. Root biometrical, histological measurements were carried out on 7-day-old and 30-day-old plants. The elevation of 5 °C affected root formation of the two lupine species differently. The initial roots of L. polyphyllus were characterized by worse spatial orientation, reduced growth and reduced mitotic index of root apical meristem at 30 °C compared with 25 °C. The length of primary roots of 30-day-old lupines and the number of lateral roots decreased by 14% and 16%, respectively. More intense root development and formation were observed in non-invasive L. luteus at 30 °C. Our results provide important information on the effect of elevated temperature on the formation of root architecture in two lupine species and suggest that global warming may impact the invasiveness of these species.

The interest in studies focused on applying probiotic microorganisms is increasing due to sustainable agriculture development. In this research, we aimed to evaluate the impact of two commercial plant probiotics—ProbioHumus and NaturGel on carrot growth, yield, and quality in organic and nonorganic production systems. The research was carried out under laboratory and field conditions. Plants were treated with probiotics (2 L/ha) at the nine leaves stage. Biometrical measurements and chemical analyses were performed at a maturation stage. The average weight of carrot roots increased by 17 and 20 g in the test variant with ProbioHumus as compared to the control in the organic and nonorganic farms, respectively. Plant microbial biostimulants ProbioHumus and NaturGel had a positive effect on the quality of carrots from organic and nonorganic farms: applied in couple they promoted the accumulation of monosaccharides, ascorbic acid, carotenoids, phenols, and increased antioxidant activity. Quantitative nitrate analysis regardless of the biostimulant used revealed about twofold lower nitrate content of carrots from organic than nonorganic farms, and probiotics did not show a significant effect on nitrate accumulation. Finally, ProbioHumus and NaturGel were effective at low doses. The use of microbial biostimulants can be recommended as an element of cultivation for creating ecologically friendly technologies.

The aim of the study was to investigate the effects of biostimulants on the resistance to freezing under laboratory-controlled cold conditions and on the growth, development, overwintering and productivity of winter rapeseed and winter wheat in natural field experiments. The effect of free amino acids, macroelements and microelements that contain biostimulants Ruter AA, Terra Sorb and Razormin was tested on cultivars of rapeseed, ‘Hornet H’, and winter wheat, ‘Skagen’ and ‘Kovas’, applying morphometrical methods. We found that biostimulants applied to rapeseed at BBCH 13–14 stage and to wheat at BBCH 14–15 stage under controlled cold stress conditions increased the freezing tolerance of seedlings. Biostimulants more actively increased the freezing resistance of rapeseed seedlings at –5°C compared to that of wheat seedlings. The temperature of –7°C was mortal to rape seedlings, while the resistance of wheat seedlings increased under the influence of the tested biostimulants compared to that of the control seedlings. In natural field experiments, these biostimulants produced a significant effect on plant growth in autumn, acclimation to the cold, plant overwintering, vegetation renewal and, due to this, formation of productivity elements. The effects of Razormin (200 mL/ha), Terra Sorb (2 L/ha) and Ruter AA (1 L/ha) were significantly higher on growth parameters of winter wheat compared to the productivity of winter rapeseed.

Cold stress is among the most important environmental factors reducing the yield of crops. The present study aimed to investigate the impact of increasing cold stress conditions on winter oilseed rape polyamines, proline, and ethylene metabolism in acclimated and non-acclimated winter oilseed rape. This study was carried out under controlled conditions in the laboratory. The winter oilseed rape hybrid ‘Visby’ was used in the experiment. Acclimated and non-acclimated plants were subjected to a two-day-long increasing cold (from −1 °C to −3 °C) treatment. HPTLC, RT-qPCR, spectral analysis, and gas chromatography methods were used to analyse the levels of polyamines, gene expression, proline, and ethylene, respectively. This study showed a decrease in putrescine, spermidine, and spermine content during cold acclimation and a decrease in putrescine and spermidine levels at sub-zero temperatures. There were intensive changes in ADC2 gene expression, proline, and ethylene levels in non-acclimated plants: a substantial increase after exposure to −1 °C temperature and a sharp decrease after exposure to −3 °C temperature. The changes in these parameters were lower or absent in acclimated plants. The phenomena observed in this study add new insights to the knowledge about the plant stress response and suggest questions to be answered in the future.

The aim of this study was to determine the influence of microbial biostimulants on wheat and oat growth, grain yield, and grain quality and to evaluate the influence of these probiotics on some soil agrochemical traits in the open field. Active concentrations of ProbioHumus and NaturGel and their mixtures were selected under laboratory conditions using winter wheat as a reference plant. Probiotics had a biostimulating effect on the development of the underground and aboveground part of winter wheat when 2 µL/g was used for seed priming and 2 mL/100 mL for seedling spraying. Under field conditions, after treatment of soil (2 L/ha), wheat and oat seeds (2 L/t), and plants (2 L/ha) with ProbioHumus and NaturGel, it was found that the yield of the studied cereals increased, on average, by 0.50 t/ha to 1.09 t/ha. ProbioHumus promoted protein accumulation in the investigated cereal grains. The level of microelements in wheat and oat grains increased after treatment of plants with NaturGel. Probiotics improved soil agrochemical properties, such as total and nitrate nitrogen, total and available phosphorus, organic carbon, humic acid, and humus content. In conclusion, plant probiotics can be used as an ecological alternative for growing cereals and improving the agrochemical properties of the soil.