Cold stress is one of the most common abiotic stresses experienced by plants and is caused by low temperature extremes and variations. Polyamines (PAs) have been reported to contribute in abiotic stress defense processes in plants. The present study investigates the survival and responses of PA-treated non-acclimated (N) and acclimated (A) winter oilseed rape to increasing cold conditions. The study was conducted under controlled conditions. Seedlings were foliarly sprayed with spermidine (Spd), spermine (Spm), and putrescine (Put) solutions (1 mM) and exposed to four days of cold acclimation (4 °C) and two days of increasing cold (from −1 to −3 °C). Two cultivars with different cold tolerance were used in this study. The recorded traits included the percentage of survival, H⁺-ATPase activity, proline accumulation, and ethylene emission. Exogenous PA application improved cold resistance, maintained the activity of plasma membrane H⁺-ATPase, increased content of free proline, and delayed stimulation of ethylene emission under increasing cold. The results of the current study on winter oilseed rape revealed that foliar application of PAs may activate a defensive response (act as elicitor to trigger physiological processes), which may compensate the negative impact of cold stress. Thus, cold tolerance of winter oilseed rape can be enhanced by PA treatment.

This study was carried out to evaluate the effect of exogenous proline on the growth, biochemical responses, and plant recovery of drought-stressed oilseed rape plants after renewed irrigation. The experiment was conducted under controlled laboratory conditions. After 21 days of cultivation, 3–4 leaf stage seedlings were sprayed with proline (1 mM), then subjected to prolonged drought stress for 8 days to achieve a severe water deficit, next, irrigation was resumed and recovery was assessed after 4 days. The results show that exogenous application of proline reduced the drought-induced growth inhibition of seedlings while maintaining relative water content (RWC) and growth parameters closer to those of irrigated plants. Proline had a positive effect on chlorophyll accumulation and membrane permeability while decreasing ethylene, H₂O₂, and MDA levels. Moreover, after 4 days of recovery, the H₂O₂ content of the proline-treated plants was significantly lower (2-fold) and the MDA content was close to that of continuously irrigated plants. Thus, all these biochemical reactions influenced plant survival: after drought + proline treatment, the number of surviving plants was two times higher than that of drought-treated plants. The findings show that exogenous proline has antioxidant, osmotic, and growth-promoting properties that improve the drought tolerance of winter oilseed rape plants and is, therefore, beneficial for drought adaptation in oilseed rape.

In order to increase plants tolerance to drought, the idea of treating them with stress-protecting compounds exogenously is being considered. In this study, we aimed to evaluate and compare the impact of exogenous calcium, proline, and plant probiotics on the response of winter wheat to drought stress. The research was carried out under controlled conditions, simulating a prolonged drought from 6 to 18 days. Seedlings were treated with ProbioHumus 2 µL g⁻¹ for seed priming, 1 mL 100 mL⁻¹ for seedling spraying, and proline 1 mM according to the scheme. 70 g m⁻² CaCO₃ was added to the soil. All tested compounds improved the prolonged drought tolerance of winter wheat. ProbioHumus, ProbioHumus + Ca had the greatest effect on maintaining the relative leaf water content (RWC) and in maintaining growth parameters close to those of irrigated plants. They delayed and reduced the stimulation of ethylene emission in drought-stressed leaves. Seedlings treated with ProbioHumus and ProbioHumus + Ca had a significantly lower degree of membrane damage induced by ROS. Molecular studies of drought-responsive genes revealed substantially lower expression of Ca and Probiotics + Ca treated plants vs. drought control. The results of this study showed that the use of probiotics in combination with Ca can activate defense reactions that can compensate for the adverse effects of drought stress.

This study evaluates the effect of end-of-day blue (EOD B) light on the physiological response of lettuce (Lactuca sativa, Lobjoits Green Cos) at different phenological development stages. Plants were grown in a controlled environment growth chamber (day/night temperature 21 ± 2 °C; relative air humidity 60 ± 5%) under the light of light-emitting diodes (LEDs) consisting of 5% blue (B; 450 nm), 85% red (R; 660 nm), and 10% green (G; 530 nm) photosynthetic photon flux density (PPFD) at 200 µmol m⁻² s⁻¹ for 16 h d⁻¹ (BRG, control) for 8, 15, and 25 days (BBCH 12, BBCH 14, and BBCH 18, respectively). For the EOD B treatments, lettuce plants were additionally illuminated with 100% of B light at 30 and 60 µmol m⁻² s⁻¹ PPFD for 4 h d⁻¹ (B₃₀ and B₆₀, respectively). The results show that EOD B light caused the elevated shoot elongation of lettuce plants regardless of their growth stages. However, leaf width increased only in more developed lettuce plants (BBCH 18). EOD B light negatively affected the development of new leaves and fresh weight, except for seedlings (BBCH 12). Most photosynthetic and spectral leaf indices also decreased when lettuce was treated with EOD B light, especially under the PPFD level of 60 µmol m⁻² s⁻¹. Moreover, the changes in metabolic parameters such as DPPH free radical activity, free proline content, and H⁺-ATPase activity in lettuce showed a plant response to unfavorable conditions to EOD B light.

This study investigates the effects of γ-aminobutyric acid (GABA) and proline, both individually and in combination, on the growth of oilseed rape under drought stress and following the resumption of irrigation. The goal was to determine whether the exogenous application of these compounds enhances the plants response to prolonged water deficit and, if so, to identify the biochemical processes involved in the plant tissue. The experiment was conducted under controlled laboratory conditions. After 21 days of plant cultivation, at the 3–4 leaf stage, seedlings were sprayed with aqueous solutions of GABA (0.1 mM) and proline (0.1 mM). The plants were then subjected to 8 days of severe drought stress, after which irrigation was resumed, and recovery was assessed over 4 days. The results showed that both amino acids alleviated the drought-induced stress as indicated by higher relative water content (RWC), increased levels of endogenous proline and photosynthetic pigments in leaves, and enhanced survival and growth recovery after drought. GABA-treated plants maintained membrane integrity and preserved plasma membrane (PM) ATPase activity during prolonged drought stress while reducing ethylene, H₂O₂, and MDA levels. Proline also influenced these biochemical responses, though to a lesser extent. The combination of GABA and proline facilitated better recovery of oilseed rape compared to the drought control group following rewatering. Notably, GABA treatment resulted in a significant increase in gene expression compared to the untreated control. Molecular analysis of drought-responsive genes revealed that the gene expression in plants treated with both proline and GABA was typically intermediate between those treated with proline alone and those treated with GABA alone. Based on these findings, we propose that GABA application could serve as an alternative to proline for improving oilseed rape’s drought tolerance, potentially increasing both crop yield and quality.

Cold stress is among the most important environmental factors reducing the yield of crops. The present study aimed to investigate the impact of increasing cold stress conditions on winter oilseed rape polyamines, proline, and ethylene metabolism in acclimated and non-acclimated winter oilseed rape. This study was carried out under controlled conditions in the laboratory. The winter oilseed rape hybrid ‘Visby’ was used in the experiment. Acclimated and non-acclimated plants were subjected to a two-day-long increasing cold (from −1 °C to −3 °C) treatment. HPTLC, RT-qPCR, spectral analysis, and gas chromatography methods were used to analyse the levels of polyamines, gene expression, proline, and ethylene, respectively. This study showed a decrease in putrescine, spermidine, and spermine content during cold acclimation and a decrease in putrescine and spermidine levels at sub-zero temperatures. There were intensive changes in ADC2 gene expression, proline, and ethylene levels in non-acclimated plants: a substantial increase after exposure to −1 °C temperature and a sharp decrease after exposure to −3 °C temperature. The changes in these parameters were lower or absent in acclimated plants. The phenomena observed in this study add new insights to the knowledge about the plant stress response and suggest questions to be answered in the future.