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Jianbin He
Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.

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Journal article
Published: 17 September 2019 in Toxins
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The aim of this research was to evaluate the potential protective mechanism of astaxanthin (ASTA) against oxidative damage and inflammation caused by ochratoxin (OTA) in mouse lung. We divided mice into a control group (CG), an OTA group (PG), an astaxanthin group (AG), and an OTA+ASTA group (JG). Oxidative indices (malondialdehyde (MDA), total superoxide dismutase (T-SOD), and reduced glutathione (GSH)) and inflammatory markers (interleukin 1β (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α)) were assayed in the lung, and the lung-weight-to-body-weight ratio was calculated. Apoptosis was detected in pathological sections by the TdT-mediated dUTP nick-end labeling (TUNEL) assay. Oxidative damage and inflammation were detected in the lung of mice after exposure to OTA. Besides, Nrf2- and NF-κB-pathway-associated proteins were detected by Western blot. In contrast with OTA, ASTA significantly raised the expression of Nrf2, HO-1, and MnSOD, while the expression of other proteins (Keap1, TLR4, and NF-κB) was significantly decreased. These results indicate that ASTA exerted protective effects against OTA-induced oxidative damage and inflammation in the lung by regulating the Nrf2 and NF-κB pathways.

ACS Style

Weixiang Xu; Mingyang Wang; Gengyuan Cui; Lin Li; Danyang Jiao; Beibei Yao; Ketao Xu; Yueli Chen; Miao Long; Shuhua Yang; Jianbin He. Astaxanthin Protects OTA-Induced Lung Injury in Mice through the Nrf2/NF-κB Pathway. Toxins 2019, 11, 540 .

AMA Style

Weixiang Xu, Mingyang Wang, Gengyuan Cui, Lin Li, Danyang Jiao, Beibei Yao, Ketao Xu, Yueli Chen, Miao Long, Shuhua Yang, Jianbin He. Astaxanthin Protects OTA-Induced Lung Injury in Mice through the Nrf2/NF-κB Pathway. Toxins. 2019; 11 (9):540.

Chicago/Turabian Style

Weixiang Xu; Mingyang Wang; Gengyuan Cui; Lin Li; Danyang Jiao; Beibei Yao; Ketao Xu; Yueli Chen; Miao Long; Shuhua Yang; Jianbin He. 2019. "Astaxanthin Protects OTA-Induced Lung Injury in Mice through the Nrf2/NF-κB Pathway." Toxins 11, no. 9: 540.

Journal article
Published: 16 August 2019 in Microorganisms
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Zearalenone (ZEA) contamination is a very serious problem around the world as it can induce reproductive disorders in animals and affect the health of humans. Therefore, reducing the damage it causes to humans and animals is a current focus of research. In this study, we assess the removing capacity of Pediococcus pentosaceus xy46 towards ZEA and investigate the mechanism responsible for its action, thus confirming if it can alleviate ZEA toxicity to the reproductive systems of male mice. Our results show that the rate at which the strain removes ZEA is as high as 89.2% in 48 h when the concentration of ZEA is 4 μg/mL in the liquid medium. Heat and acid treatment significantly enhanced the ability of the bacteria to remove ZEA. The animal experiments results show that the oral administration of xy46 to mice (0.2 mL daily at a concentration of 109 CFU/mL for 28 days) significantly reduces the degree of testicular pathomorphological changes and apoptosis induced by ZEA when the mice are intragastric administration with 40 mg/kg ZEA daily for 28 days. Moreover, oral administration of xy46 enhances the decrease in the testosterone level and improves the oxidative stress injury induced by ZEA. Furthermore, oral administration of xy46 reverts the expression of these genes and proteins in the testicular tissues of the mice involved in the blood–testis barrier and apoptosis (e.g., Vim, caspase 12, Cldn11, N-cad, Bax, and Bcl-2). However, xy46 cannot significantly revert in some of these evaluated parameters, especially in sperm quantity and quality when the mice were given 70 mg/kg ZEA daily for 28 days. In conclusion, our results suggest that the strain Pediococcus pentosaceus xy46 can efficiently remove ZEA from the liquid medium, the mechanism responsible for its action is absorption, and it can alleviate the toxicity of ZEA to the reproductive systems of male mice when the mice are given 40 mg/kg ZEA daily, However, it cannot completely alleviate the reproductive toxicity of higher dosage of zearalenone through its ability to adsorb ZEA.

ACS Style

Shuhua Yang; Ping Gong; Jianwen Pan; Wang; Jingjing Tong; Miao Long; Peng Li; Jianbin He; Yang; Gong; Pan; Tong; Long; Li; He; Nan Wang; Mingyang Wang. Pediococcus pentosaceus xy46 Can Absorb Zearalenone and Alleviate its Toxicity to the Reproductive Systems of Male Mice. Microorganisms 2019, 7, 266 .

AMA Style

Shuhua Yang, Ping Gong, Jianwen Pan, Wang, Jingjing Tong, Miao Long, Peng Li, Jianbin He, Yang, Gong, Pan, Tong, Long, Li, He, Nan Wang, Mingyang Wang. Pediococcus pentosaceus xy46 Can Absorb Zearalenone and Alleviate its Toxicity to the Reproductive Systems of Male Mice. Microorganisms. 2019; 7 (8):266.

Chicago/Turabian Style

Shuhua Yang; Ping Gong; Jianwen Pan; Wang; Jingjing Tong; Miao Long; Peng Li; Jianbin He; Yang; Gong; Pan; Tong; Long; Li; He; Nan Wang; Mingyang Wang. 2019. "Pediococcus pentosaceus xy46 Can Absorb Zearalenone and Alleviate its Toxicity to the Reproductive Systems of Male Mice." Microorganisms 7, no. 8: 266.

Journal article
Published: 01 February 2019 in International Journal of Molecular Sciences
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Cadmium (Cd) is harmful for humans and animals, especially for the reproductive system. However, the mechanism of its toxicity has not been elucidated, and how to alleviate its toxicity is very important. This study aimed to explore the role and mechanism of action of sulforaphane (SFN) in protecting mouse Leydigs (TM3) cells from cadmium (Cd)-induced damage. The half-maximal inhibitory concentration (IC50) of Cd and the safe doses of SFN were determined using a methyl thiazolyl tetrazolium (MTT) assay. The testosterone secretion from TM3 cells was measured using the enzyme-linked immunosorbent assay. The intracellular oxidative stress was evaluated using corresponding kits. The cell apoptosis was detected using flow cytometry. The mRNA expression of genes associated with NF-E2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling was detected using reverse transcription–polymerase chain reaction, including Nrf2, heme oxygenase I (HO-1), glutathione peroxidase (GSH-Px), NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), and γ-glutamylcysteine synthetase (γ-GCS). The protein expression of Nrf2, GSH-Px, HO-1, γ-GCS, and NQO1 was detected using Western blot analysis. The results showed that the IC50 of Cd to TM3 cells was 51.4 µmol/L. SFN reduced the release of lactate dehydrogenase from Cd-exposed cells. Cd + SFN 2.5 treatment significantly elevated testosterone concentration compared with the Cd group (p < 0.05). SFN significantly increased total superoxide dismutase (T-SOD) and GSH-Px activity and GSH content in Cd-treated cells (p < 0.05; p < 0.01), inhibited the production of malondialdehyde or reactive oxygen species caused by Cd (p < 0.05; p < 0.01), and reduced the apoptotic rate of Cd-induced TM3 cells (p < 0.01). SFN upregulated the mRNA expression of Nrf2, GSH-Px, HO-1, NQO1, and γ-GCS in Cd-treated cells, indicating the protective effect of SFN against Cd-induced oxidative stress or cell apoptosis by activating the Nrf2/ARE signaling pathway.

ACS Style

Shu-Hua Yang; Peng Li; Li-Hui Yu; Lin Li; Miao Long; Ming-Da Liu; Jian-Bin He. Sulforaphane Protect Against Cadmium-Induced Oxidative Damage in mouse Leydigs cells by Activating Nrf2/ARE Signaling Pathway. International Journal of Molecular Sciences 2019, 20, 630 .

AMA Style

Shu-Hua Yang, Peng Li, Li-Hui Yu, Lin Li, Miao Long, Ming-Da Liu, Jian-Bin He. Sulforaphane Protect Against Cadmium-Induced Oxidative Damage in mouse Leydigs cells by Activating Nrf2/ARE Signaling Pathway. International Journal of Molecular Sciences. 2019; 20 (3):630.

Chicago/Turabian Style

Shu-Hua Yang; Peng Li; Li-Hui Yu; Lin Li; Miao Long; Ming-Da Liu; Jian-Bin He. 2019. "Sulforaphane Protect Against Cadmium-Induced Oxidative Damage in mouse Leydigs cells by Activating Nrf2/ARE Signaling Pathway." International Journal of Molecular Sciences 20, no. 3: 630.

Journal article
Published: 31 October 2018 in Toxins
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Zearalenone (ZEN) is an estrogen-like mycotoxin produced by Fusarium that seriously compromises the safety of animal and human health. In this study, our aim was to evaluate the protective effect of Bacillus velezensis A2 against biochemical and pathological changes induced by zearalenone in mice. Kunming mice (n = 40; 25 ± 2 g) were allotted to four treatment groups: a control group (basic feed); a ZEN group (basic feed with a ZEN dose of 60 mg/kg); an A2 strain fermented feed group (150 g of feed mixed with 150 mL of sterile distilled water and inoculated with 5 mL of phosphate buffer salt (PBS) resuspended A2 strain); and an A2 strain fermented ZEN-contaminated feed group. (A2 strain group 150 mL pure bacterial distilled water system mixed with 150 g ZEN-contaminated feed.) Our results showed that the Bacillus velezensis A2 strain can completely degrade the ZEN-contaminated feed within 5 days. (The concentration of ZEN in fermentation was 60 μg/mL.) After the mice fed for 28 days, compared with the control group, the activities of AST and ALT were increased, the activities of glutathione peroxidase (GSH-PX) and total superoxide dismutase (T-SOD) were decreased, and the amount of creatinine (CRE), blood urea nitrogen (BUN), uric acid (UA), and malondialdehyde (MDA) in the ZEN group were increased in the mice serum (p < 0.05; p < 0.01). However, compared with the ZEN group, these biochemical levels were reversed in the A2 strain fermented feed group and in the A2 strain fermented ZEN-contaminated feed group (p < 0.05; p < 0.01). Furthermore, histopathological analysis only showed pathological changes of the mice liver in the ZEN group. The results showed that Bacillus velezensis A2 as additive could effectively remove ZEN contamination in the feed and protect the mice against the toxic damage of ZEN. In conclusion, Bacillus velezensis A2 has great potential use as a microbial feed additive to detoxify the toxicity of zearalenone in production practice.

ACS Style

Nan Wang; Peng Li; Mingyang Wang; Si Chen; Sheng Huang; Miao Long; Shuhua Yang; Jianbin He. The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice. Toxins 2018, 10, 449 .

AMA Style

Nan Wang, Peng Li, Mingyang Wang, Si Chen, Sheng Huang, Miao Long, Shuhua Yang, Jianbin He. The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice. Toxins. 2018; 10 (11):449.

Chicago/Turabian Style

Nan Wang; Peng Li; Mingyang Wang; Si Chen; Sheng Huang; Miao Long; Shuhua Yang; Jianbin He. 2018. "The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice." Toxins 10, no. 11: 449.