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Marieke Rüth
XcorLab GmbH, Industrie Center Obernburg, 63784 Obernburg am Main, Germany

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Journal article
Published: 03 June 2021 in Sensors
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In this article, we summarize our investigations on optimized 248 nm deep ultraviolet (UV) fabrication of highly stable epoxy polymer Bragg grating sensors and their application for biomedical purposes. Employing m-line spectroscopy, deep UV photosensitivity of cross-linked EpoCore thin films in terms of responding refractive index change is determined to a maximum of Δn = + (1.8 ± 0.2) × 10−3. All-polymer waveguide Bragg gratings are fabricated by direct laser irradiation of lithographic EpoCore strip waveguides on compatible Topas 6017 substrates through standard +1/-1-order phase masks. According near-field simulations of realistic non-ideal phase masks provide insight into UV dose-dependent characteristics of the Bragg grating formation. By means of online monitoring, arising Bragg reflections during grating inscription via beforehand fiber-coupled waveguide samples, an optimum laser parameter set for well-detectable sensor reflection peaks in respect of peak strength, full width at half maximum and grating attenuation are derived. Promising blood analysis applications of optimized epoxy-based Bragg grating sensors are demonstrated in terms of bulk refractive index sensing of whole blood and selective surface refractive index sensing of human serum albumin.

ACS Style

Steffen Hessler; Marieke Rüth; Horst-Dieter Lemke; Bernhard Schmauss; Ralf Hellmann. Deep UV Formation of Long-Term Stable Optical Bragg Gratings in Epoxy Waveguides and Their Biomedical Sensing Potentials. Sensors 2021, 21, 3868 .

AMA Style

Steffen Hessler, Marieke Rüth, Horst-Dieter Lemke, Bernhard Schmauss, Ralf Hellmann. Deep UV Formation of Long-Term Stable Optical Bragg Gratings in Epoxy Waveguides and Their Biomedical Sensing Potentials. Sensors. 2021; 21 (11):3868.

Chicago/Turabian Style

Steffen Hessler; Marieke Rüth; Horst-Dieter Lemke; Bernhard Schmauss; Ralf Hellmann. 2021. "Deep UV Formation of Long-Term Stable Optical Bragg Gratings in Epoxy Waveguides and Their Biomedical Sensing Potentials." Sensors 21, no. 11: 3868.

Randomized controlled trial
Published: 16 January 2019 in Toxins
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The protein-bound uremic toxins para-cresyl sulfate (pCS) and indoxyl sulfate (IS) are associated with cardiovascular disease in chronic renal failure, but the effect of different dialysis procedures on their plasma levels over time is poorly studied. The present prospective, randomized, cross-over trial tested dialysis efficacy and monitored pre-treatment pCS and IS concentrations in 15 patients on low-flux and high-flux hemodialysis and high-convective volume postdilution hemodiafiltration over six weeks each. Although hemodiafiltration achieved by far the highest toxin removal, only the mean total IS level was decreased at week three (16.6 ± 12.1 mg/L) compared to baseline (18.9 ± 13.0 mg/L, p = 0.027) and to low-flux dialysis (20.0 ± 12.7 mg/L, p = 0.021). At week six, the total IS concentration in hemodiafiltration reached the initial values again. Concentrations of free IS and free and total pCS remained unaltered. Highest beta2-microglobulin elimination in hemodiafiltration (p < 0.001) led to a persistent decrease of the plasma levels at week three and six (each p < 0.001). In contrast, absent removal in low-flux dialysis resulted in rising beta2-microglobulin concentrations (p < 0.001). In conclusion, this trial demonstrated that even large differences in instantaneous protein-bound toxin removal by current extracorporeal dialysis techniques may have only limited impact on IS and pCS plasma levels in the longer term.

ACS Style

Detlef H. Krieter; Simon Kerwagen; Marieke Rüth; Horst-Dieter Lemke; Christoph Wanner. Differences in Dialysis Efficacy Have Limited Effects on Protein-Bound Uremic Toxins Plasma Levels over Time. Toxins 2019, 11, 47 .

AMA Style

Detlef H. Krieter, Simon Kerwagen, Marieke Rüth, Horst-Dieter Lemke, Christoph Wanner. Differences in Dialysis Efficacy Have Limited Effects on Protein-Bound Uremic Toxins Plasma Levels over Time. Toxins. 2019; 11 (1):47.

Chicago/Turabian Style

Detlef H. Krieter; Simon Kerwagen; Marieke Rüth; Horst-Dieter Lemke; Christoph Wanner. 2019. "Differences in Dialysis Efficacy Have Limited Effects on Protein-Bound Uremic Toxins Plasma Levels over Time." Toxins 11, no. 1: 47.

Journal article
Published: 01 October 2018 in Optics & Laser Technology
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We report on cellular adhesion of human primary fibroblasts on scaffolds fabricated by laser-induced two-photon polymerization using 515 nm femtosecond pulses. The scaffolds are large scaled with a dimension in the range of several millimeters and consist of a periodic reproducible structure. A minimum process duration of 6.3 min is achieved by an implemented nonstop single-line single-pass fabrication process and allows to write several identical specimens with different pore sizes from 10 µm up to 90 µm suitable for cell adhesion studies in a reasonable amount of time. OrmoComp®, an organic-inorganic hybrid polymer, is chosen as base material for the structures. Human dermal fibroblasts are directly seeded on scaffolds after several post-processing steps to ensure the extraction of toxic residues. Cell adhesion, proliferation, and survival are examined after three, six, and nine days of culture, respectively. Cell growth is compared depending on the different pore sizes of the scaffolds. Due to the horizontal and vertical cell growth observed on and inside the structures, we demonstrate that large scaffolds prepared from OrmoComp® qualify for three-dimensional cell adhesion and growth without support of an additional protein coating.

ACS Style

Anika Trautmann; Marieke Rüth; Horst-Dieter Lemke; Thomas Walther; Ralf Hellmann. Two-photon polymerization based large scaffolds for adhesion and proliferation studies of human primary fibroblasts. Optics & Laser Technology 2018, 106, 474 -480.

AMA Style

Anika Trautmann, Marieke Rüth, Horst-Dieter Lemke, Thomas Walther, Ralf Hellmann. Two-photon polymerization based large scaffolds for adhesion and proliferation studies of human primary fibroblasts. Optics & Laser Technology. 2018; 106 ():474-480.

Chicago/Turabian Style

Anika Trautmann; Marieke Rüth; Horst-Dieter Lemke; Thomas Walther; Ralf Hellmann. 2018. "Two-photon polymerization based large scaffolds for adhesion and proliferation studies of human primary fibroblasts." Optics & Laser Technology 106, no. : 474-480.

Journal article
Published: 16 May 2018 in Therapeutic Apheresis and Dialysis
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ACS Style

Tomasz Piątek; Joanna Giebultowicz; Marieke Rüth; Horst-Dieter Lemke; Florian Bonn; Piotr Wroczynski; Marta Hrenczuk; Piotr Malkowski; Jacek Rozga. Albumin Apheresis for Artificial Liver Support: In Vitro Testing of a Novel Filter. Therapeutic Apheresis and Dialysis 2018, 22, 399 -409.

AMA Style

Tomasz Piątek, Joanna Giebultowicz, Marieke Rüth, Horst-Dieter Lemke, Florian Bonn, Piotr Wroczynski, Marta Hrenczuk, Piotr Malkowski, Jacek Rozga. Albumin Apheresis for Artificial Liver Support: In Vitro Testing of a Novel Filter. Therapeutic Apheresis and Dialysis. 2018; 22 (4):399-409.

Chicago/Turabian Style

Tomasz Piątek; Joanna Giebultowicz; Marieke Rüth; Horst-Dieter Lemke; Florian Bonn; Piotr Wroczynski; Marta Hrenczuk; Piotr Malkowski; Jacek Rozga. 2018. "Albumin Apheresis for Artificial Liver Support: In Vitro Testing of a Novel Filter." Therapeutic Apheresis and Dialysis 22, no. 4: 399-409.

Conference paper
Published: 02 January 2018 in Nanophotonics Australasia 2017
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We report on the fabrication of three-dimensional direct laser written scaffolds for tissue engineering and the seeding of primary fibroblasts on these structures. Scaffolds are realized by two-photon absorption induced polymerization in the inorganic-organic hybrid polymer OrmoComp using a 515 nm femtosecond laser. A nonstop single-line single-pass writing process is implemented in order to produce periodic reproducible large scaled structures with a dimension in the range of several millimeters and reduce process time to less than one hour. This method allows us to determine optimized process parameters for writing stable structures while achieving pore sizes ranging from 5 μm to 90 μm and a scanning speed of up to 5 mm/s. After a multi-stage post-treatment, normal human dermal fibroblasts are applied to the scaffolds to test if these macroscopic structures with large surface and numerous small gaps between the pores provide nontoxic conditions. Furthermore, we study the cell behavior in this environment and observe both cell growth on as well as ingrowth on the three-dimensional structures. In particular, fibroblasts adhere and grow also on the vertical walls of the scaffolds.

ACS Style

Anika Trautmann; Horst-Dieter Lemke; Ralf Hellmann; Thomas Walther; Marieke Rüth. Large 3D direct laser written scaffolds for tissue engineering applications. Nanophotonics Australasia 2017 2018, 10456, 104565A .

AMA Style

Anika Trautmann, Horst-Dieter Lemke, Ralf Hellmann, Thomas Walther, Marieke Rüth. Large 3D direct laser written scaffolds for tissue engineering applications. Nanophotonics Australasia 2017. 2018; 10456 ():104565A.

Chicago/Turabian Style

Anika Trautmann; Horst-Dieter Lemke; Ralf Hellmann; Thomas Walther; Marieke Rüth. 2018. "Large 3D direct laser written scaffolds for tissue engineering applications." Nanophotonics Australasia 2017 10456, no. : 104565A.

Journal article
Published: 01 November 2016 in European Journal of Pharmacology
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The limited removal of metabolic waste products in dialyzed kidney patients leads to high morbidity and mortality. One powerful solution for a more complete removal of those metabolites might be offered by a bioartificial kidney device (BAK), which contains a hybrid "living membrane" with functional proximal tubule epithelial cells (PTEC). These cells are supported by an artificial functionalized hollow fiber membrane (HFM) and are able to actively remove the waste products. In our earlier studies, conditionally immortalized human PTEC (ciPTEC) showed to express functional organic cationic transporter 2 (OCT2) when seeded on small size flat or hollow fiber polyethersulfone (PES) membranes. Here, an upscaled "living membrane" is presented. We developed and assessed the functionality of modules containing three commercially available MicroPES HFM supporting ciPTEC. The HFM were optimally coated with L-Dopa and collagen IV to support a uniform and tight monolayer formation of matured ciPTEC under static culturing conditions. Both abundant expression of zonula occludens-1 (ZO-1) protein and limited diffusion of FITC-inulin confirm a clear barrier function of the monolayer. Furthermore, the uptake of 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP), a fluorescent OCT2 substrate, was studied in absence and presence of known OCT inhibitors, such as cimetidine and a cationic uremic solutes mixture. The ASP uptake by the living upscaled membrane was decreased by 60% in the presence of either inhibitor, proving the active function of OCT2. In conclusion, this study presents a successful upscaling of a living membrane with active organic cation transport as a support for BAK device.

ACS Style

Natalia Vladimirovna Chevtchik; Michele Fedecostante; Jitske Jansen; Milos Mihajlovic; Martijn Wilmer; Marieke Rüth; Rosalinde Masereeuw; Dimitrios Stamatialis. Upscaling of a living membrane for bioartificial kidney device. European Journal of Pharmacology 2016, 790, 28 -35.

AMA Style

Natalia Vladimirovna Chevtchik, Michele Fedecostante, Jitske Jansen, Milos Mihajlovic, Martijn Wilmer, Marieke Rüth, Rosalinde Masereeuw, Dimitrios Stamatialis. Upscaling of a living membrane for bioartificial kidney device. European Journal of Pharmacology. 2016; 790 ():28-35.

Chicago/Turabian Style

Natalia Vladimirovna Chevtchik; Michele Fedecostante; Jitske Jansen; Milos Mihajlovic; Martijn Wilmer; Marieke Rüth; Rosalinde Masereeuw; Dimitrios Stamatialis. 2016. "Upscaling of a living membrane for bioartificial kidney device." European Journal of Pharmacology 790, no. : 28-35.

Randomized controlled trial
Published: 22 June 2016 in Acta Physiologica
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Protein-bound uraemic toxin accumulation causes uraemia associated cardiovascular morbidity. Enhancing the plasma ionic strength releases toxins from protein binding and makes them available for removal during dialysis. This concept was implemented through high sodium concentrations ([Na+]) in the substituate of predilution haemodiafiltration at increased plasma ionic strength (HDF-IPIS). Ex vivo HDF-IPIS with blood tested increasing [Na+] to demonstrate efficacy and haemocompatibility. Haemocompatibility was further assessed in sheep using two different HDF-IPIS setups and [Na+] between 350 and 600 mmol/L. Safety and efficacy of para-cresyl sulfate (pCS) and indoxyl sulfate (IS) removal was further investigated in a randomized clinical pilot trial comparing HDF-IPIS to HD and HDF. Compared to [Na+] of 150 mmol/L, ex vivo HDF-IPIS at 500 mmol/L demonstrated up to 50 % higher IS removal. Haemolysis in sheep was low even at [Na+] of 600 mmol/L (free Hb 0.016 ± 0.001 g/dL). In patients, compared to HD, a [Na+] of 240 mmol/L in HDF-IPIS resulted in 40 % greater reduction (48.7 ± 23.6 vs. 67.8 ± 7.9 %; P=0.013) of free IS. Compared to HD and HDF (23.0 ± 14.8 and 25.4 ± 10.5 mL/min), the dialytic clearance of free IS was 31.6 ± 12.8 mL/min (P=0.017) in HDF-IPIS, but [Na+] in arterial blood increased from 132 ± 2 to 136 ± 3 mmol/L (0 vs. 240 min; P<0.001). HDF-IPIS is technically and clinically feasible. More effective HDF-IPIS requires higher temporary plasma [Na+], but dialysate [Na+] has to be appropriately adapted to avoid sodium accumulation. This article is protected by copyright. All rights reserved.

ACS Style

Detlef H. Krieter; Eric Devine; Thomas Körner; Marieke Rüth; Christoph Wanner; Manuelle Raine; Joachim Jankowski; Horst‐Dieter Lemke. Haemodiafiltration at increased plasma ionic strength for improved protein-bound toxin removal. Acta Physiologica 2016, 219, 510 -520.

AMA Style

Detlef H. Krieter, Eric Devine, Thomas Körner, Marieke Rüth, Christoph Wanner, Manuelle Raine, Joachim Jankowski, Horst‐Dieter Lemke. Haemodiafiltration at increased plasma ionic strength for improved protein-bound toxin removal. Acta Physiologica. 2016; 219 (2):510-520.

Chicago/Turabian Style

Detlef H. Krieter; Eric Devine; Thomas Körner; Marieke Rüth; Christoph Wanner; Manuelle Raine; Joachim Jankowski; Horst‐Dieter Lemke. 2016. "Haemodiafiltration at increased plasma ionic strength for improved protein-bound toxin removal." Acta Physiologica 219, no. 2: 510-520.

Journal article
Published: 01 May 2014 in Nephrology Dialysis Transplantation
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Introduction and Aims: Klotho is an “aging-suppressor” gene and encodes a single-pass transmembrane protein predominantly expressed in renal tubules. Whether chronic kidney disease (CKD) affects serum Klotho is poorly documented. We aimed to measure the relationship of serum α-Klotho with renal function, acid-base status, bone biomarkers and proteinuria in CKD patients.

ACS Style

Valerie Hage; Solenne Pelletier; Laurence Dubourg; Jocelyne Drai; Charlotte Cuerq; Sandrine Lemoine; Aoumeur Hadj-Aissa; Maurice Laville; Denis Fouque; S. Chinnappa; L. B. Tan; A. Mooney; A. M. El Nahas; G. Glorieux; R. Vanholder; E. White; Joachim Jankowski; Doreen Janke; Marieke Rüth; Horst-Dieter Lemke; Vera Jankowski; Thomas Troeger; Matthias Wessely; Martin Bidlingmaier; Ulf Schönermarck; Nino Hadjamu; Simon Rau; Michael Fischereder; Yaeni Kim; Y. A. Hong; Min Young Kim; Ji Hee Lim; Yoon Sik Chang; Cheol Whee Park. UREMIC TOXICITY. Nephrology Dialysis Transplantation 2014, 29, iii19 -iii20.

AMA Style

Valerie Hage, Solenne Pelletier, Laurence Dubourg, Jocelyne Drai, Charlotte Cuerq, Sandrine Lemoine, Aoumeur Hadj-Aissa, Maurice Laville, Denis Fouque, S. Chinnappa, L. B. Tan, A. Mooney, A. M. El Nahas, G. Glorieux, R. Vanholder, E. White, Joachim Jankowski, Doreen Janke, Marieke Rüth, Horst-Dieter Lemke, Vera Jankowski, Thomas Troeger, Matthias Wessely, Martin Bidlingmaier, Ulf Schönermarck, Nino Hadjamu, Simon Rau, Michael Fischereder, Yaeni Kim, Y. A. Hong, Min Young Kim, Ji Hee Lim, Yoon Sik Chang, Cheol Whee Park. UREMIC TOXICITY. Nephrology Dialysis Transplantation. 2014; 29 (suppl 3):iii19-iii20.

Chicago/Turabian Style

Valerie Hage; Solenne Pelletier; Laurence Dubourg; Jocelyne Drai; Charlotte Cuerq; Sandrine Lemoine; Aoumeur Hadj-Aissa; Maurice Laville; Denis Fouque; S. Chinnappa; L. B. Tan; A. Mooney; A. M. El Nahas; G. Glorieux; R. Vanholder; E. White; Joachim Jankowski; Doreen Janke; Marieke Rüth; Horst-Dieter Lemke; Vera Jankowski; Thomas Troeger; Matthias Wessely; Martin Bidlingmaier; Ulf Schönermarck; Nino Hadjamu; Simon Rau; Michael Fischereder; Yaeni Kim; Y. A. Hong; Min Young Kim; Ji Hee Lim; Yoon Sik Chang; Cheol Whee Park. 2014. "UREMIC TOXICITY." Nephrology Dialysis Transplantation 29, no. suppl 3: iii19-iii20.

Journal article
Published: 24 January 2014 in Toxins
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Protein binding prevents uremic toxins from removal by conventional extracorporeal therapies leading to accumulation in maintenance dialysis patients. Weakening of the protein binding may enhance the dialytic elimination of these toxins. In ultrafiltration and equilibrium dialysis experiments, different measures to modify the plasma binding affinity and capacity were tested: (i), increasing the sodium chloride (NaCl) concentration to achieve a higher ionic strength; (ii), increasing the temperature; and (iii), dilution. The effects on the dissociation constant KD and the protein bound fraction of the prototypical uremic toxin indoxyl sulfate (IS) in plasma of healthy and uremic individuals were studied. Binding of IS corresponded to one site binding in normal plasma. KD increased linearly with the NaCl concentration between 0.15 (KD = 13.2 ± 3.7 µM) and 0.75 M (KD = 56.2 ± 2.0 µM). Plasma dilution further reduced the protein bound toxin fraction by lowering the protein binding capacity of the plasma. Higher temperatures also decreased the protein bound fraction of IS in human plasma. Increasing the NaCl concentration was effective to weaken the binding of IS also in uremic plasma: the protein bound fraction decreased from 89% ± 3% to 81% ± 3% at 0.15 and 0.75 M NaCl, respectively. Dilution and increasing the ionic strength and temperature enhance the free fraction of IS allowing better removal of the substance during dialysis. Applied during clinical dialysis, this may have beneficial effects on the long-term outcome of maintenance dialysis patients.

ACS Style

Eric Devine; Detlef H. Krieter; Marieke Rüth; Joachim Jankovski; Horst-Dieter Lemke. Binding Affinity and Capacity for the Uremic Toxin Indoxyl Sulfate. Toxins 2014, 6, 416 -429.

AMA Style

Eric Devine, Detlef H. Krieter, Marieke Rüth, Joachim Jankovski, Horst-Dieter Lemke. Binding Affinity and Capacity for the Uremic Toxin Indoxyl Sulfate. Toxins. 2014; 6 (2):416-429.

Chicago/Turabian Style

Eric Devine; Detlef H. Krieter; Marieke Rüth; Joachim Jankovski; Horst-Dieter Lemke. 2014. "Binding Affinity and Capacity for the Uremic Toxin Indoxyl Sulfate." Toxins 6, no. 2: 416-429.

Research article
Published: 07 March 2013 in PLOS ONE
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Metabolic stimuli, pressure, and fluid shear stress (FSS) are major mediators of vascular plasticity. The exposure of the vessel wall to increased laminar FSS is the main trigger of arteriogenesis, the remodelling of pre-existent arterio-arteriolar anastomoses to functional conductance arteries. In this study, we have used an in vitro bioreactor to investigate cell-specific interactions, molecular mechanisms as well as time-dependent effects under laminar FSS conditions. This bioreactor termed “artificial artery” can be used for screening potential arterio-protective substances, pro-arteriogenic factors, and for investigating biomarkers of cardiovascular diseases such as cardiac diseases. The bioreactor is built up out of 14 hollow fiber membranes colonized with endothelial cells (HUVECs) on the inside and smooth muscle cells (HUASMCs) on the outside. By means of Hoechst 33342 staining as well as immunocytochemistry of ß-catenin and α-smooth-muscle-actin, a microporous polypropylene membrane was characterized as being the appropriate polymer for co-colonization. Defined arterial flow conditions (0.1 N/m2 and 3 N/m2), metabolic exchange, and cross-talk of HUVECs and HUASMCs through hollow fibers mimic physiological in vivo conditions of the vasculature. Analysing mono- and co-culture secretomes by MALDI-TOF-TOF mass spectrometry, we could show that HUVECs secreted Up4A upon 3 N/m2. A constant cellular secretion of randomly chosen peptides verified viability of the “artificial artery” for a cultivation period up to five days. qRT-PCR analyses revealed an up-regulation of KLF2 and TIMP1 as mechano-regulated genes and demonstrated arterio-protective, homeostatic FSS conditions by a down-regulation of EDN1. Expression analyses of VWF and EDN1 furthermore confirmed that RNA of both cell types could separately be isolated without cross-contamination. CCND1 mRNA expression in HUVECs did not change upon FSS indicating a quiescent endothelial phenotype. Taken together, the “artificial artery” provides a solid in vitro model to test pharmacological active compounds for their impact on arterio-damaging or arterio-protective properties on vascular response.

ACS Style

Doreen Janke; Joachim Jankowski; Marieke Rüth; Ivo Buschmann; Horst-Dieter Lemke; Dorit Jacobi; Petra Knaus; Ernst Spindler; Walter Zidek; Kerstin Lehmann; Vera Jankowski. The “Artificial Artery” as In Vitro Perfusion Model. PLOS ONE 2013, 8, e57227 .

AMA Style

Doreen Janke, Joachim Jankowski, Marieke Rüth, Ivo Buschmann, Horst-Dieter Lemke, Dorit Jacobi, Petra Knaus, Ernst Spindler, Walter Zidek, Kerstin Lehmann, Vera Jankowski. The “Artificial Artery” as In Vitro Perfusion Model. PLOS ONE. 2013; 8 (3):e57227.

Chicago/Turabian Style

Doreen Janke; Joachim Jankowski; Marieke Rüth; Ivo Buschmann; Horst-Dieter Lemke; Dorit Jacobi; Petra Knaus; Ernst Spindler; Walter Zidek; Kerstin Lehmann; Vera Jankowski. 2013. "The “Artificial Artery” as In Vitro Perfusion Model." PLOS ONE 8, no. 3: e57227.