The objective of this study was to illustrate the photosynthetic characteristics of current twigs of Salix matsudana Koidz., and clarify the effect of stem photosynthesis on the new organ development in cutting seedlings. Excised twigs were taken as the experimental samples. The response of the stem photosynthesis rate to increasing light intensity and the effective photochemical efficiency of the cross section of the twig were determined. Then, twigs were used as cuttings and exposed to 0, 20, and 100 μmol m⁻² s⁻¹ light intensities, respectively, to achieve distinctive stem photosynthetic rates. After 14 days of treatment, stem water and non-structural carbohydrate (NSC) content, as well as the biomass and carbon isotopic composition, of new organs in the cutting seedlings under different light treatments were examined. The results showed that the gross photosynthetic rate significantly increased within 400 μmol m⁻² s⁻¹ of light intensity, and the maximum rate was approximately 1.27 μmol m⁻² s⁻¹. The effective photochemical efficiency of the PSⅡ of the cortex was significantly higher than the inner tissues in the cross section of the twig. When twig cuttings were exposed to different light intensities, stem water and starch content, as well as bud and root biomass, were significantly higher in the cutting seedling subjected to 100 μmol m⁻² s⁻¹ than the case treated in darkness; however, the bud δ¹³C trend was the opposite. Stem photosynthesis played a positive role in the maintenance of stem water and starch supply for the cutting seedlings, and ¹³C depleted assimilates produced by stem photosynthesis contributed to bud biomass, revealing that stem photosynthesis promotes organ development in cutting seedlings of Salix matsudana.

This study is among the first to investigate ozone levels in urban forests in China. It establishes that urban forest air quality in Yuanshan Forest Park (Shenzhen) is suitable for recreational activities and identifies spatial, seasonal, and diurnal O₃ patterns and relationships with micrometeorological parameters, suggesting the possibility of manipulating relevant forest characteristics to reduce Surface ozone (O₃) levels. An understanding of O₃ levels of urban forest environments is needed to assess potential effects on human health and recreational activities. Such studies in China are scarce. This study investigated urban forest O₃ levels to improve understanding and support residents engaging in forest recreational activities. We monitored O₃ levels in 2015–2016 for three urban forests representing common habitats (foothill, valley, and ridge) in Yuanshan Forest Park and for an adjacent square. The overall mean daily and daily maximum 8 h mean (MDA8) O₃ concentrations were highest for the ridge forest and lowest for the valley forest. Each forest’s O₃ concentrations were highest in summer. Diurnally, forest O₃ concentrations peaked between 13:00 and 17:00 and reached a minimum between 03:00 and 09:00. The correlation between forest O₃ concentrations and air temperature (AT) was strongly positive in summer and autumn but negative in spring. In each season, O₃ concentration was negatively correlated with relative humidity (RH). No MDA8 or hourly O₃ concentrations in the forests exceeded National Ambient Air Quality Standard Grade I thresholds (100 and 160 μg m⁻³, respectively). O₃ accumulation is present in ridge urban forest in all seasons. Foothill and valley urban forests have better air quality than ridge forestation. Urban forest air quality is better in spring and autumn than in summer and is better from night-time to early morning than from noon to afternoon.

Bark chloroplasts play important roles in carbon balancing by recycling internal stem CO₂ into assimilated carbon. The photosynthetic response of bark chloroplasts to interior stem environments has been studied recently in woody plants. However, the molecular regulatory mechanisms underlying specific characteristics of bark photosynthesis remain unclear. To address this knowledge gap, differences in the structure, photosynthetic activity and protein expression profiles between bark and leaf chloroplasts were investigated in Salix matsudana in this study. Bark chloroplasts exhibited broader and lower grana stacks and higher levels of starch relative to leaf chloroplasts. Concomitantly, decreased oxygen evolution rates and decreased saturated radiation point were observed in bark chloroplasts. Furthermore, a total of 293 differentially expressed proteins (DEPs) were identified in bark and leaf chloroplast profile comparisons. These DEPs were significantly enriched in photosynthesis-related biological processes or Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with photosynthesis. All 116 DEPs within the KEGG pathways associated with photosynthesis light reactions were downregulated in bark chloroplasts, including key proteins responsible for chlorophyll synthesis, light energy harvesting, nonphotochemical quenching, linear electron transport and photophosphorylation. Interestingly, seven upregulated proteins involved in dark reactions were identified in bark chloroplasts that comprised two kinds of malic enzymes typical of C₄-type photosynthesis. These results provide comprehensive proteomic evidence to understand the low photochemical capability of bark chloroplasts and suggest that bark chloroplasts might fix CO₂ derived from malate decarboxylation.

Research Highlights: This study is among the first to investigate ozone levels in urban forests in China. It establishes that urban forest air quality in Yuanshan Forest Park, Shenzhen, is suitable for recreational activities and identifies spatial, seasonal, and diurnal O3 patterns and relationships with micrometeorological parameters, suggesting the possibility of manipulating relevant forest characteristics to reduce O3 levels. Background and Objectives: An understanding of O3 levels of urban forest environments is needed to assess potential effects on human health and recreational activities. Such studies in China are scarce. This study investigated urban forest O3 levels to improve understanding and support residents engaging in forest recreational activities. Materials and Methods: We monitored O3 levels in 2015–2016 for three urban forests representing common habitats (foothill, valley, and ridge) in Yuanshan Forest Park, Shenzhen, and for an adjacent square. Results: The overall mean daily and daily maximum 8-h mean (MDA8) O3 concentrations were highest for the ridge forest and lowest for the valley forest. Each forest’s O3 concentrations were highest in summer. Diurnally, forest O3 concentrations peaked between 13:00 and 17:00 and reached a minimum between 03:00 and 09:00. The correlation between forest O3 concentrations and air temperature (AT) was strongly positive in summer and autumn but negative in spring. In each season, O3 concentration was negatively correlated with relative humidity (RH). No MDA8 or hourly O3 concentrations in the forests exceeded National Ambient Air Quality Standard Grade I thresholds (100 and 160 μg m−3, respectively). Conclusions: O3 accumulation is present in ridge urban forest in all seasons. Foothill and valley urban forests have better air quality than ridge forestation. Urban forest air quality is better in spring and autumn than in summer and is better from night-time to early morning than from noon to afternoon.

Urban green spaces, closely tied to local climates, significantly affect human comfort levels, yet existing assessment methods vary in applicability across different contexts and regions. Here, we determined the applicability of two commonly used indices to evaluate human comfort in urban green space types in Hohhot City in China, which is in an arid and semi-arid area. We established sites in four different urban green space types (S1–S4) and a control area (CK) through field-based assessment, and collected meteorological data over 10 days in each season from 2020 to 2021. Specifically, air temperature, relative humidity, and average wind speed were observed from 7:00 to 19:00. Air temperature was highest in summer and lowest in winter. Throughout the day, air temperature first increased and then decreased, with the maximum temperature occurring later in winter than in other seasons. Relative humidity showed an opposite diurnal trend to temperature, and there were no significant differences between urban green space types and CK. The average wind speed of CK was significantly higher than that of the urban green space types. HCI_Lu classifies thermal comfort levels across urban green space types and seasons into four distinct categories as uncomfortable, comfortable to less comfortable, less comfortable, and extremely uncomfortable. HCI_CMA further stratifies thermal conditions at urban green space types by season into cool and refreshing, most comfortable, most comfortable to slightly cool, cold, and uncomfortable. The HCI_Lu ranged from 2.3 to 25.1, and tended to first decrease and then increase on a daily basis. Conversely, HCI_CMA fluctuated throughout the day and ranged from 18.6 to 78.0. According to HCI_Lu, the urban green space types were comfortable for 45% of the observation time, and were comfortable for a greater proportion of time compared to if the comfort was calculated using HCI_CMA. HCI_CMA was strongly correlated with air temperature and average wind speed. According to receiver operating characteristic (ROC) curve analysis, the area under the curve (AUC) for HCI_CMA was 0.59–0.91, and was higher than that of HCI_Lu in each season, indicating greater suitability for the study site.