Glutathione and aged garlic extract are sulfur-containing products that play important protective and regulatory roles within the immune system and in oxidative processes. Hydrogen sulfide (H₂S), an endogenous, gaseous, signaling transmitter, has also been shown to be involved in the regulation of inflammation. Recent studies have shown that sulfur-containing compounds from garlic have beneficial effects in attenuating outcomes associated with cardiovascular disease and inflammation by a mechanism that may be related to the H₂S signaling pathway. In this review, we summarize the main functions of glutathione (GSH), garlic derivatives and H₂S and their role in the immune response and impact on health and disease.

Surface water poses a great risk to fruit and vegetable crops when contaminated by foodborne pathogens. Several factors impact the microbial quality of surface waters and increase the risk of produce contamination. Therefore, evaluating the factors associated with the prevalence of pathogenic microorganisms in agricultural water sources is critical to determine and establish preventive actions that may minimize the incidence of foodborne outbreaks associated with contaminated production water. In the Southeastern U.S. environmental factors such as rainfall, temperature, and seasonal variations have been associated with the prevalence of pathogens or microbial indicators of fecal contamination in water. Also, the geographical location of the irrigation sources as well as surrounding activities and land use play an important role on the survival and prevalence of pathogenic bacteria. Therefore, these factors may be determinants useful in the evaluation of production water quality and may help to preemptively identify scenarios or hazards associated with the incidence of foodborne pathogenic microorganisms.

Growers commonly wash fresh produce with chemical sanitizers during postharvest handling. However, these sanitizers can be harsh to washing systems and pose a health risk to workers. Essential oils (EOs) can be used as alternatives to chemical sanitizers in produce washing. Previous studies reveal that the EOs from thyme, oregano, cinnamon, and clove are the main EOs evaluated in the studies as potential sanitizers for the washing of produce. The use of EOs and surfactants, such as tween80 and cetylpyridinium chloride, might be used to improve the antimicrobial activity of emulsions. However, studies are still required to evaluate the potential effect of different chemical components of EOs and preparations. Also, it is recommended that researchers focus on overcoming obstacles regarding EOs application in washing systems, including the high levels of EO required to reduce bacterial growth, undesired organoleptic impact on produce, and the poor solubility of EOs in aqueous solution.

The use of unmanned aerial application systems (UAASs) for precision pesticide applications has increased alongside the demand for sustainable agricultural practices. However, limited studies have standardized the necessary flight parameters ensuring the optimal use of UAASs in specialty crops (e.g., fruits and vegetables). Thus, the objective of this study was to evaluate the effects of flight speed, droplet size, and application volume on the spray deposition of UAASs, creating guidelines to facilitate their use in specialty crops. Field experiments were conducted in a three-factorial experimental design of three flight speeds (i.e., 4, 7, and 10 m/s), three droplet sizes (i.e., 150, 250, and 350 µm), and two application volumes (i.e., 18.75 and 28.10 L/ha). Spraying droplet parameters (i.e., coverage, droplet density, and droplet spectra, and application uniformity), measured through the effective swath width, were recorded to assess spray deposition efficiency. Flight speed, droplet size, and application volume significantly influenced spray deposition. Treatments with slower flight speeds (4 m/s) and higher application volumes (28.10 L/ha) increased spray coverage, while droplet density was maximized at 4 m/s with the finest droplet size (150 µm), which are desirable characteristics for pesticide applications in specialty crops. Ultimately, the effective swath width and spray uniformity were maximized at a flight speed of 7.93 m/s with a droplet size of 350 µm. These results help optimize UAAS-based pesticide application, increasing efficiency and reducing environmental impact; however, understanding pesticide translocation dynamics (i.e., systemic or contact) on plants is key for growers to determine flight parameters.

Terpenes play a vital role in plant defense; tomato plants produce a diverse range of terpenes within specialized glandular trichomes, influencing interactions with herbivores, predators, and pollinators. This study employed two distinct methods, namely leaf dip and maceration, to extract trichomes from tomato leaves. Terpene quantification was carried out using Gas Chromatography -Mass Spectrometry (GC -MS). The leaf dip method proved effective in selectively targeting trichome content, revealing unique extraction patterns compared to maceration. The GC -MS method demonstrated high linearity, accuracy, sensitivity, and low limits of detection and quantification. Application of the method to different tomato species ( Solanum pennellii, Solanum pimpinellifolium, Solanum galapagense, Solanum habrochaites , and Solanum lycopersicum ) identified significant variation in terpene content among these species, highlighting the potential of specific accessions for breeding programs. Notably, the terpene alpha-zingiberene, known for its repellency against whiteflies, was found in high quantities (211.90 -9155.13 mu g g -1 ) in Solanum habrochaites accession PI209978. These findings provide valuable insights into terpenoid diversity for plant defense mechanisms, guiding future research on developing pest -resistant tomato cultivars. Additionally, the study underscores the broader applications of terpenes in agriculture.

Essential oils (EOs) have strong antibacterial properties and can be potential sanitizers to reduce pathogen load and prevent cross-contamination during postharvest washing. The objective of this study was to investigate the efficacy of emulsions containing oregano (OR; Origanum vulgare) and winter savory (WS; Satureja montana) EOs at different concentrations (0.94 and 1.88 µL/mL) and storage times (0 h, 24 h, and 7 days), in reducing Escherichia coli O157:H7 on the surface of three types of lettuce (romaine, crisphead, and butterhead). The EO emulsions were compared with one no-rinse treatment and three rinse treatments using water, 200 ppm chlorine, and 80 ppm peroxyacetic acid (PAA), respectively, in a simulated washing system. The results showed that while the EO emulsions significantly reduced E. coli O157:H7 on crisphead lettuce over time, not all treatments were effective for romaine and butterhead lettuce. The mixture of OR and WS at concentrations of 0.94 and 1.88 µL/mL was found to be the most effective in reducing E. coli O157:H7 on inoculated lettuce, resulting in reductions of 3.52 and 3.41 log CFU/g, respectively. Furthermore, the PAA and the mixture of OR and WS at 1.88 µL/mL effectively limited bacterial cross-contamination close to the detection limit for all lettuce types during all storage times. These results suggest that OR and WS EOs could serve as potential alternatives to chemical sanitizers for postharvest lettuce washing.