The number of cotton plants grown per unit of ground area has gained attention due to the high prices of inputs and lower production. Cotton yield per unit of area in Henan province has been stagnant in the last few years. The objectives of this study were to investigate cotton growth, yield, boll spatial distribution and biomass accumulation using different plant densities at cultivation and to find out the optimal plant density. A 2-year field experiment was conducted in a randomized complete block design under six plant densities (D1, 15,000; D2, 33,000; D3, 51,000; D4, 69,000; D5, 87,000 and D6, 105,000 ha⁻¹). Cotton grown at lower plant density produced taller plants and high number of leaves per plant while greater number of branches, fruiting nodes and high number of bolls per unit of ground area were produced under high plant density. Boll retention rate decreased as plant population increased and at nodes 1–8 the rate decreased slowly and then increased dramatically. The highest seed cotton yield (4546 kg ha⁻¹) and lint yield (1682 kg ha⁻¹) was produced by D5. The seed cotton and lint yield produced by D5 were 51–55%, 40–37%, 22–26%, 11–15%, 12–15%, 28–30%, 21–24%, 15–20%, 7–13% and 13–17% higher than D1, D2, D3, D4 and D6 during both years of experimentation, respectively. The increase in seed yield was due to higher biomass accumulation in reproductive organs under D5 plant density. The highest average (110.4 V_A kg ha⁻¹ d⁻¹) and maximum (126 V_M kg ha⁻¹ d⁻¹) rate of reproductive organs biomass was also accumulated by D5 as compared to other plant densities. The results suggest that D5 is the optimal plant density for high reproductive biomass accumulation and high yield for the area of Henan province.

The objective of this study was to assess the impacts of nitrogen on the physiological characteristics of the source–sink system of upper fruiting branches under various amounts of nitrogen fertilization. A two-year field experiment was conducted with a Bt cotton cultivar in the Yellow River Basin of China. The growth and yield of cotton of the upper fruiting branches were compared under four nitrogen levels: Control (N0, 0 kg ha⁻¹), low nitrogen (N1, 120 kg ha⁻¹), moderate nitrogen (N2, 240 kg ha⁻¹), and high nitrogen (N3, 480 kg ha⁻¹). The results indicated that in the subtending leaves in upper fruiting branches, chlorophyll content, protein content, and peroxidase (POD) activity dramatically increased with nitrogen application, reaching the highest under the moderate nitrogen treatment. The physiological characters in the seeds had the same trends as in the subtending leaves. Furthermore, the moderate nitrogen rate (240 kg ha⁻¹) had a favorable yield and quality. Our results supported that a moderate nitrogen rate (240 kg ha⁻¹) could coordinate the source–sink growth of cotton in the late stage, enhance the yield and fiber quality, and decrease the cost of fertilizer in the Yellow River Basin of China and other similar ecological areas.

Modification of the cotton canopy results in shade avoidance and competition for light, which shows that density and spatial arrangement of cotton have a great impact on light interception. This experiment was conducted in 2018 and 2019 in the experimental field at the Institute of Cotton Research of Chinese Academy of Agricultural Science in Anyang city, Henan Province, China. Six plant densities of cotton variety SCRC28 were used to assess spatial competition for light in cotton populations during the whole growing period. Light interception data were collected and analyzed according to the spatial grid method and the extension of Simpson’s 3/8 rule. The results showed that at the bottom of the canopy, greater light interception was observed at high densities than at low densities, while in the external part of the layer of the canopy in the horizontal direction, low light interception was recorded at low densities. Leaf area, aboveground biomass and plant height were obviously correlated with light interception, and the cotton population with a higher density (8.7 plants m⁻²) performed best at the light interception competition, and with the highest yield. The results will provide guidance on light management through the optimization of the structure of the canopy to provide more solar radiation and a significant basis by which to improve the management of light and canopy architecture.