Remote estimation of Sun‐induced chlorophyll fluorescence emitted by terrestrial vegetation can provide an unparalleled opportunity to track spatiotemporal variations of photosynthetic efficiency. Here we provide the first direct experimental evidence that the two peaks of the chlorophyll fluorescence spectrum can be accurately mapped from high‐resolution radiance spectra and that the signal is linked to variations in actual photosynthetic efficiency. Red and far red fluorescence measured using a novel airborne imaging spectrometer over a grass carpet treated with an herbicide known to inhibit photosynthesis was significantly higher than the corresponding signal from an equivalent untreated grass carpet. The reflectance signal of the two grass carpets was indistinguishable, confirming that the fast dynamic changes in fluorescence emission were related to variations in the functional status of actual photosynthesis induced by herbicide application. Our results from a controlled experiment at the local scale illustrate the potential for the global mapping of terrestrial photosynthesis through space‐borne measurements of chlorophyll fluorescence.

Advances in high-performance computer resources and exploitation of high-density terrestrial laser scanning (TLS) data allow for reconstruction of close-to-reality 3D forest scenes for use in canopy radiative transfer models. Consequently, our main objectives were (i) to reconstruct 3D representation of Norway spruce (Picea abies) trees by deriving distribution of woody and foliage elements from TLS and field structure data and (ii) to use the reconstructed 3D spruce representations for evaluation of the effects of canopy structure on forest reflectance simulated in the Discrete Anisotropic Radiative Transfer (DART) model. Data for this study were combined from two spruce research sites located in the mountainous areas of the Czech Republic. The canopy structure effects on simulated top-of-canopy reflectance were evaluated for four scenarios (10 × 10 m scenes with 10 trees), ranging from geometrically simple to highly detailed architectures. First scenario A used predefined simple tree crown shapes filled with a turbid medium with simplified trunks and branches. Other three scenarios used the reconstructed 3D spruce representations with B detailed needle shoots transformed into turbid medium, C with simplified shoots retained as facets, and D with detailed needle shoots retained as facets D. For the first time, we demonstrated the capability of the DART model to simulate reflectance of complex coniferous forest scenes up to the level of a single needle (scenario D). Simulated bidirectional reflectance factors extracted for each scenario were compared with actual airborne hyperspectral and space-borne Sentinel-2 MSI reflectance data. Scenario A yielded the largest differences from the remote sensing observations, mainly in the visible and NIR regions, whereas scenarios B, C, and D produced similar results revealing a good agreement with the remote sensing data. When judging the computational requirements for reflectance simulations in DART, scenario B can be considered as most operational spruce forest representation, because the transformation of 3D shoots in turbid medium reduces considerably the simulation time and hardware requirements.

Synergies of optical, thermal and laser scanning remotely sensed data provide valuable information to study the structure and functioning of terrestrial ecosystems. One of the few fully operational airborne multi-sensor platforms for ecosystem research in Europe is the Flying Laboratory of Imaging Systems (FLIS), operated by the Global Change Research Institute of the Czech Academy of Sciences. The system consists of three commercial imaging spectroradiometers. One spectroradiometer covers the visible and near-infrared, and the other covers the shortwave infrared part of the electromagnetic spectrum. These two provide full spectral data between 380–2450 nm, mainly for the assessment of biochemical properties of vegetation, soil and water. The third spectroradiometer covers the thermal long-wave infrared part of the electromagnetic spectrum and allows for mapping of surface emissivity and temperature properties. The fourth instrument onboard is the full waveform laser scanning system, which provides data on landscape orography and 3D structure. Here, we describe the FLIS design, data acquisition plan and primary data pre-processing. The synchronous acquisition of multiple data sources provides a complex analytical and data framework for the assessment of vegetation ecosystems (such as plant species composition, plant functional traits, biomass and carbon stocks), as well as for studying the role of greenery or blue-green infrastructure on the thermal behaviour of urban systems. In addition, the FLIS airborne infrastructure supports calibration and validation activities for existing and upcoming satellite missions (e.g., FLEX, PRISMA).

This study presents a method for three-dimensional (3D) reconstruction of forest tree species that are, for instance, required for simulations of 3D canopies in radiative transfer modelling. We selected three forest species of different architecture: Norway spruce (Picea abies) and European beech (Fagus sylvatica), representatives of European production forests, and white peppermint (Eucalyptus pulchella), a common forest species of Tasmania. Each species has a specific crown structure and foliage distribution. Our algorithm for 3D model construction of a single tree is based on terrestrial laser scanning (TLS) and ancillary field measurements of leaf angle distribution, percentage of current-year and older leaves, and other parameters that could not be derived from TLS data. The algorithm comprises four main steps: (i) segmentation of a TLS tree point cloud separating wooden parts from foliage, (ii) reconstruction of wooden parts (trunks and branches) from TLS data, (iii) biologically genuine distribution of foliage within the tree crown and (iv) separation of foliage into two age categories (for spruce trees only). The reconstructed 3D models of the tree species were used to build virtual forest scenes in the Discrete Anisotropic Radiative Transfer model and to simulate canopy optical signals, specifically: angularly anisotropic top-of-canopy reflectance (for retrieval of leaf biochemical compounds from nadir canopy reflectance signatures captured in airborne imaging spectroscopy data) and solar-induced chlorophyll fluorescence signal (for experimentally unfeasible sensitivity analyses).

Scaling leaf-level optical signals to the canopy level is essential for airborne and satellite-based forest monitoring. In evergreen trees, biophysical and optical traits may change as foliage ages. This study aims to evaluate the effect of age in Norway spruce needle on biophysical trait-prediction based on laboratory leaf-level spectra. Mature Norway spruce trees were sampled at forest stands in ten headwater catchments with different soil properties. Foliage biophysical traits (pigments, phenolics, lignin, cellulose, leaf mass per area, water, and nitrogen content) were assessed for three needle-age classes. Complementary samples for needle reflectance and transmittance were measured using an integrating sphere. Partial least square regression (PLSR) models were constructed for predicting needle biophysical traits from reflectance—separating needle age classes and assessing all age classes together. The ten study sites differed in soil properties rather than in needle biophysical traits. Optical properties consistently varied among age classes; however, variation related to the soil conditions was less pronounced. The predictive power of PLSR models was needle-age dependent for all studied traits. The following traits were predicted with moderate accuracy: needle pigments, phenolics, leaf mass per area and water content. PLSR models always performed better if all needle age classes were included (rather than individual age classes separately). This also applied to needle-age independent traits (water and lignin). Thus, we recommend including not only current but also older needle traits as a ground truth for evergreen conifers with long needle lifespan.