The detection of vertical heterogeneity in vegetation has attracted an increasing attention as it has a great significance for precise agriculture. The hyperspectral light detection and ranging (LiDAR) (HSL) can obtain the spectral and spatial information simultaneously. However, its ability to monitor the vertical distribution of biochemical parameters in plants has not been fully explored. In this article, the applicability of empirical ratio and normalized spectral indices for HSL channels in chlorophyll (Chl) detection was investigated using three data sets: the PROSPECT-5 synthetic data set, the ANGERS public data set, and an HSL-measured data set. A linear regression model of the best performing index against measured Chl values was constructed so as to build 3-D Chl point clouds of maize. The performance of HSL in Chl detection at the upper and lower layers was also tested based on the selected spectral index. The result showed that the CIred edge index was most compatible with the HSL channels. The estimated Chl concentrations of the upper and lower layers showed the close relationships with HSL measurements (R² = 0.73 and 0.91, respectively). The vertical Chl profiles in maize were also presented, indicating that the HSL system has a strong ability to monitor the vertical distribution of maize Chl concentrations. This article provides a basis for the vertical detection of vegetation biochemical parameters directly from HSL measurements.

Terrestrial hyperspectral LiDAR (HSL) sensors could provide not only spatial information of the measured targets but also the backscattered spectral intensity signal of the laser pulse. The raw intensity collected by HSL is influenced by several factors, among which the range, incidence angle and sub-footprint play a significant role. Further studies on the influence of the range, incidence angle and sub-footprint are needed to improve the accuracy of backscatter intensity data as it is important for vegetation structural and biochemical information estimation. In this paper, we investigated the effects on the laser backscatter intensity and developed a practical correction method for HSL data. We established a laser ratio calibration method and a reference target-based method for HSL and investigated the calibration procedures for the mixed measurements of the effects of the incident angle, range and sub-footprint. Results showed that the laser ratio at the red-edge and near-infrared laser wavelengths has higher accuracy and simplicity in eliminating range, incident angle and sub-footprint effects and can significantly improve the backscatter intensity discrepancy caused by these effects.

As a novel remote sensor, hyperspectral LiDAR is faced with the incidence angle effect, which restricts its quantitative applications. However, the current radiometric correction algorithms have some limitations, concentrating on: 1) the mathematically polynomial fitting; 2) adjacent wavelength ratio such as ratio vegetation index; and 3) perfect Lambertian assumption and using the Lambert cosine law to correct the effect. In this study, a practical and proper correction algorithm is proposed to overcome these limitations. First, to better characterize the complex reflection characteristics of the object surface, a combination of the Lambert law and Beckmann law is applied to represent the object surface. Then, it considers the impact of both wavelength and incidence angle on describing the surface roughness factor and diffuse fraction. Finally, a modified and physically based radiometric correction algorithm is generated. It provides the detailed correction equations for intensity and reflectance data recorded by hyperspectral LiDAR. To obtain its parameters and verify its reliability, leaves (ten samples per species, 30 in total) were stochastically collected from three broadleaf trees to experiment. The results showed that the algorithm achieved good performances by comparing the intensity and reflectance changes before and after removing the leaf incidence angle effect. Since it is physically based, the algorithm could promisingly be a fundamental solution to eliminate the incidence angle effect for hyperspectral LiDAR.

As hyperspectral LiDAR (HSL) combines the advantages of hyperspectral remote sensing and LiDAR, it has the potential of estimating vegetation biochemical contents at any three-dimensional (3-D) location. We investigate the capability of HSL to monitor leaf chlorophyll and nitrogen contents at a distance of 7.5 m. Using full-waveform LiDAR data obtained by HSL, the performance of the partial least square regression (PLSR) model using three strategies (only wavelength bands, only vegetation indices, and both wavelength bands and vegetation indices) is explored based on the correlation analysis of 16 wavelength bands and 16 vegetation indices. The result shows that the PLSR model can make full use of various wavelength bands and vegetation indices, having a strong ability to monitor leaf chlorophyll and nitrogen contents using full-waveform LiDAR data. The analysis can be applied for predicting leaf biochemical components of other vegetation and provides the basis for monitoring 3-D distribution of biochemical contents using HSL in the future.

Advanced remote sensing techniques for estimating crop nitrogen (N) are crucial for optimizing N fertilizer management. Hyperspectral LiDAR (HSL) data, with both spectral and spatial information of the targets, can extract more plant properties than traditional LiDAR and hyperspectral imaging systems. In this study, we tested the ability of HSL in terms of estimating maize N concentration at the leaf-level by using spectral indices and partial least squares regression (PLSR) methods. Subsequently, the N estimation was scaled up to the plant-level based on HSL point clouds. Biomass, extracted with structural proxies, was utilized to exhibit its supplemental effect on N concentration. The results show that HSL has the ability to extract N concentrations at both the leaf-level and the canopy-level, and PLSR showed better performance (R² > 0.6) than the single spectral index (R² > 0.4). In comparison to the stem height and maximum canopy width, the plant height had the strongest ability (R² = 0.88) to estimate biomass. Future research should utilize larger datasets to test the viability of using HSL to monitor the N concentration of crops, which is beneficial for precision agriculture.