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Huaiying Yao
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China

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Journal article
Published: 24 August 2021 in Microorganisms
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Intestinal bacteria are crucial for the healthy aquaculture of Litopenaeus vannamei, and the coastal areas of China are important areas for concentrated L. vannamei cultivation. In this study, we evaluated different compositions and structures, key roles, and functional potentials of the intestinal bacterial community of L. vannamei shrimp collected in 12 Chinese coastal cities and investigated the correlation between the intestinal bacteria and functional potentials. The dominant bacteria in the shrimp intestines included Proteobacteria, Bacteroidetes, Tenericutes, Firmicutes, and Actinobacteria, and the main potential functions were metabolism, genetic information processing, and environmental information processing. Although the composition and structure of the intestinal bacterial community, potential pathogenic bacteria, and spoilage organisms varied from region to region, the functional potentials were homeostatic and significantly (p< 0.05) correlated with intestinal bacteria (at the family level) to different degrees. The correlation between intestinal bacteria and functional potentials further suggested that L. vannamei had sufficient functional redundancy to maintain its own health. These findings help us understand differences among the intestinal bacterial communities of L. vannamei cultivated in different regions and provide a basis for the disease management and healthy aquaculture of L. vannamei.

ACS Style

Yimeng Cheng; Chaorong Ge; Wei Li; Huaiying Yao. The Intestinal Bacterial Community and Functional Potential of Litopenaeus vannamei in the Coastal Areas of China. Microorganisms 2021, 9, 1793 .

AMA Style

Yimeng Cheng, Chaorong Ge, Wei Li, Huaiying Yao. The Intestinal Bacterial Community and Functional Potential of Litopenaeus vannamei in the Coastal Areas of China. Microorganisms. 2021; 9 (9):1793.

Chicago/Turabian Style

Yimeng Cheng; Chaorong Ge; Wei Li; Huaiying Yao. 2021. "The Intestinal Bacterial Community and Functional Potential of Litopenaeus vannamei in the Coastal Areas of China." Microorganisms 9, no. 9: 1793.

Journal article
Published: 31 July 2021 in Agriculture
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Zinc oxide nanoparticles (ZnO NPs) are widely used and exposed to the soil environment, but their effect on soil nitrous oxide (N2O) emissions remains unclear. In this study, a microcosm experiment was conducted to explore the effects of different ZnO NPs concentrations (0, 100, 500, and 1000 mg kg−1) on N2O emissions and associated functional genes related to N2O amendment with carbon (C) or nitrogen (N) substrates. Partial least squares path modeling (PLS-PM) was used to explore possible pathways controlling N2O emissions induced by ZnO NPs. In the treatment without C or N substrates, 100 and 500 mg kg−1 ZnO NPs did not affect N2O production, but 1000 mg kg−1 ZnO NPs stimulated N2O production. Interestingly, compared with the soils without ZnO NPs, the total N2O emissions in the presence of different ZnO NPs concentrations increased by 2.36–4.85-, 1.51–1.62-, and 6.28–8.35-fold following C, N and both C & N substrate amendments, respectively. Moreover, ZnO NPs increased the functional genes of ammonia-oxidizing bacteria (AOB amoA) and nitrite reductase (nirS) and led to the exhaustion of nitrate but reduced the gene copies of ammonia-oxidizing archaea (AOA amoA). In addition, the redundancy analysis results showed that the AOB amoA and nirS genes were positively correlated with total N2O emissions, and the PLS-PM results showed that ZnO NPs indirectly affected N2O emissions by influencing soil nitrate content, nitrifiers and denitrifiers. Overall, our results showed that ZnO NPs increase N2O emissions by increasing nitrification (AOB amoA) and denitrification (nirS), and we highlight that the exposure of ZnO NPs in agricultural fields probably results in a high risk of N2O emissions when coupled with C and N substrate amendments, contributing to global climate warming.

ACS Style

Ziyi Feng; Yongxiang Yu; Huaiying Yao; Chaorong Ge. Effect of Zinc Oxide Nanoparticles on Nitrous Oxide Emissions in Agricultural Soil. Agriculture 2021, 11, 730 .

AMA Style

Ziyi Feng, Yongxiang Yu, Huaiying Yao, Chaorong Ge. Effect of Zinc Oxide Nanoparticles on Nitrous Oxide Emissions in Agricultural Soil. Agriculture. 2021; 11 (8):730.

Chicago/Turabian Style

Ziyi Feng; Yongxiang Yu; Huaiying Yao; Chaorong Ge. 2021. "Effect of Zinc Oxide Nanoparticles on Nitrous Oxide Emissions in Agricultural Soil." Agriculture 11, no. 8: 730.

Review
Published: 29 June 2021 in Animals
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Functional antimicrobial peptides (AMPs) are an important class of effector molecules of innate host immune defense against pathogen invasion. Inability of microorganisms to develop resistance against the majority of AMPs has made them alternatives to antibiotics, contributing to the development of a new generation of antimicrobials. Due to extensive biodiversity, insects are one of the most abundant sources of novel AMPs. Notably, black soldier fly insect (BSF; Hermetia illucens (Diptera: Stratiomyidae)) feeds on decaying substrates and displays a supernormal capacity to survive under adverse conditions in the presence of abundant microorganisms, therefore, BSF is one of the most promising sources for identification of AMPs. However, discovery, functional investigation, and drug development to replace antibiotics with AMPs from Hermetia illucens remain in a preliminary stage. In this review, we provide general information on currently verified AMPs of Hermetia illucens, describe their potential medical value, discuss the mechanism of their synthesis and interactions, and consider the development of bacterial resistance to AMPs in comparison with antibiotics, aiming to provide a candidate for substitution of antibiotics in livestock farming or, to some extent, for blocking the horizontal transfer of resistance genes in the environment, which is beneficial to human and animal welfare.

ACS Style

Jing Xia; Chaorong Ge; Huaiying Yao. Antimicrobial Peptides from Black Soldier Fly (Hermetia illucens) as Potential Antimicrobial Factors Representing an Alternative to Antibiotics in Livestock Farming. Animals 2021, 11, 1937 .

AMA Style

Jing Xia, Chaorong Ge, Huaiying Yao. Antimicrobial Peptides from Black Soldier Fly (Hermetia illucens) as Potential Antimicrobial Factors Representing an Alternative to Antibiotics in Livestock Farming. Animals. 2021; 11 (7):1937.

Chicago/Turabian Style

Jing Xia; Chaorong Ge; Huaiying Yao. 2021. "Antimicrobial Peptides from Black Soldier Fly (Hermetia illucens) as Potential Antimicrobial Factors Representing an Alternative to Antibiotics in Livestock Farming." Animals 11, no. 7: 1937.

Review
Published: 31 May 2021 in CATENA
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The use of plastic film in agriculture strongly affects the decomposition of soil organic carbon (SOC) and the release of methane (CH4) and nitrous oxide (N2O) by influencing soil biogeochemical processes. However, no systematic conclusions have been reached regarding the effect of plastic film mulching on SOC stocks and greenhouse gas (GHG) budgets. In this study, a meta-analysis compiling data from 150 studies was used to assess the effects of film mulching on SOC and soil GHG fluxes across different agricultural systems. In addition, the net global warming potentials (GWPs) of SOC, CH4 and N2O were calculated with the GWP approach. Plastic film mulching significantly promoted crop yield by 48.6%; did not affect SOC; reduced CH4 emissions from paddy fields by 64.2% and CH4 uptake in uplands by 16.1%; and increased soil N2O emissions by 23.9%. The effect of mulching on SOC and GHG fluxes varied among agricultural systems. Compared with the non-mulched field, the use of plastic film significantly increased SOC and decreased CH4 uptake in rainfed uplands but reduced SOC in irrigated uplands. In paddy fields, this practice significantly reduced the SOC stock and CH4 emissions but increased N2O emissions. Our results suggest that a low coverage ratio (<50%), black film mulching and flat mulching effectively increase SOC levels compared to those in non-mulched fields, and a low coverage ratio, biodegradable film and ridge mulching could mitigate the positive effect of this practice on N2O emissions. Overall, our results suggest that plastic film mulching promotes crop productivity and slightly affects the net GWP in uplands. In paddy fields, this technology appears to be a “win-win” strategy for crop production and GHG mitigation, but additional practices (e.g., biochar application) should be adopted to maintain SOC levels for sustainable development in these fields because it greatly reduces SOC stocks.

ACS Style

Yongxiang Yu; Yanxia Zhang; Mao Xiao; Chengyi Zhao; Huaiying Yao. A meta-analysis of film mulching cultivation effects on soil organic carbon and soil greenhouse gas fluxes. CATENA 2021, 206, 105483 .

AMA Style

Yongxiang Yu, Yanxia Zhang, Mao Xiao, Chengyi Zhao, Huaiying Yao. A meta-analysis of film mulching cultivation effects on soil organic carbon and soil greenhouse gas fluxes. CATENA. 2021; 206 ():105483.

Chicago/Turabian Style

Yongxiang Yu; Yanxia Zhang; Mao Xiao; Chengyi Zhao; Huaiying Yao. 2021. "A meta-analysis of film mulching cultivation effects on soil organic carbon and soil greenhouse gas fluxes." CATENA 206, no. : 105483.

Journal article
Published: 15 May 2021 in Science of The Total Environment
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The anaerobic oxidation of methane (AOM) mediated by microorganisms is a key process in the reduction of methane emissions, and AOM-coupled electron acceptors have been shown to regulate methane emissions into the atmosphere in marine systems. Paddy fields are a significant source of methane and account for 20% of global methane emissions, but the effect of electron acceptors on the methane emission process in flooded paddy fields has been poorly characterized. This study aimed to determine whether the electron acceptors ferric iron and nitrate, and biochar, acting as an electron shuttle, can regulate the AOM process in paddy soil, with or without interaction between biochar and these two electron acceptors. We also aimed to characterize which microorganisms are actively involved. Here, we added 13C-labeled CH4 (13CH4) into anaerobic microcosms to evaluate the role of electron acceptors by measuring the methane oxidation rate and the enrichment of 13C-labeled CO2 (13CO2). We then combined DNA-stable isotope probing with amplicon sequencing to study the active microorganisms. We found for the first time that, in addition to nitrate, ferric iron can also effectively promote AOM in paddy soil. However, there was no significant effect of biochar. Ferric iron-dependent AOM was mainly carried out by iron-reducing bacteria (Geobacter, Ammoniphilus and Clostridium), and nitrate-dependent AOM was mainly by nitrate-reducing bacteria (Rhodanobacter, Paenibacillus and Planococcus). Our results demonstrate that the AOM process, regulated by the electron acceptors ferric iron and nitrate, can alleviate methane emission from paddy soil. The potentially active microorganisms related to electron acceptor reduction may be crucial for this methane sink and deserve further research.

ACS Style

Dan Luo; Xiangtian Meng; Ningguo Zheng; Yaying Li; Huaiying Yao; Stephen J. Chapman. The anaerobic oxidation of methane in paddy soil by ferric iron and nitrate, and the microbial communities involved. Science of The Total Environment 2021, 788, 147773 .

AMA Style

Dan Luo, Xiangtian Meng, Ningguo Zheng, Yaying Li, Huaiying Yao, Stephen J. Chapman. The anaerobic oxidation of methane in paddy soil by ferric iron and nitrate, and the microbial communities involved. Science of The Total Environment. 2021; 788 ():147773.

Chicago/Turabian Style

Dan Luo; Xiangtian Meng; Ningguo Zheng; Yaying Li; Huaiying Yao; Stephen J. Chapman. 2021. "The anaerobic oxidation of methane in paddy soil by ferric iron and nitrate, and the microbial communities involved." Science of The Total Environment 788, no. : 147773.

Journal article
Published: 17 April 2021 in Forests
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Soil bacterial microbial communities are important in the ecosystem function and succession of forests. Using high-throughput 16S rRNA gene sequencing and relative importance for linear regression, we explored how the structures of soil bacterial community were influenced by the environmental factors and restoration succession of secondary forests in the Miyaluo Mountains of western Sichuan, China. Using a space-for-time approach, field measurements and sampling were conducted in four stands at different stages of natural restoration. Results of distance-based multivariate analysis showed that soil pH, organic carbon, available phosphorus, and C/N ratio were the predominant environmental factors that collectively explained a 46.9% variation in the bacterial community structures. The community compositions were jointly controlled by the direct and indirect effects of the rehabilitation stages. The changes in soil environmental factors coincided with restoration succession could lead to the shifts in the relative abundance of different soil bacterial taxa. We screened 13 successional discriminant taxa that could quantitatively indicate the secondary succession subalpine stage. Collectively, our findings show that soil bacteria in different taxa are governed by different local soil variables and rehabilitation ages, which can lead to shifts in the relative abundance of different taxa in successional stages, ultimately changing the entire soil bacterial community with the succession of secondary forest.

ACS Style

Zheliang Sheng; Wanze Zhu; Huaiying Yao; Shumiao Shu; Xia Li; Shenglan Ma; Yaying Li; Jinbo Xiong. Niche Selection by Soil Bacterial Community of Disturbed Subalpine Forests in Western Sichuan. Forests 2021, 12, 505 .

AMA Style

Zheliang Sheng, Wanze Zhu, Huaiying Yao, Shumiao Shu, Xia Li, Shenglan Ma, Yaying Li, Jinbo Xiong. Niche Selection by Soil Bacterial Community of Disturbed Subalpine Forests in Western Sichuan. Forests. 2021; 12 (4):505.

Chicago/Turabian Style

Zheliang Sheng; Wanze Zhu; Huaiying Yao; Shumiao Shu; Xia Li; Shenglan Ma; Yaying Li; Jinbo Xiong. 2021. "Niche Selection by Soil Bacterial Community of Disturbed Subalpine Forests in Western Sichuan." Forests 12, no. 4: 505.

Book chapter
Published: 18 January 2021 in Methods in Microbiology
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In the past two decades, DNA/RNA-stable isotope probing (SIP) techniques have been widely used in microbial ecology to decipher the link between microbial metabolic function and phylogenetic information. Diverse methodological strategies were employed to guarantee the successful SIP experiments. In view of the emerging literature and our experience in the optimization of methods for DNA/RNA-SIP, it is crucial to provide a methodological comparison. This review discusses methods published on DNA/RNA SIP, considering a range of experimental parameters, including the selection of nucleic acid, labelled element, substrate amount, incubation time, the speed and time of isopycnic gradient centrifugation, and the detection approach of labelled fractions and microbes after ultracentrifugation. Moreover, some scopes of environmental microorganism studied through 13C-DNA/RNA-SIP technology, such as microbes assimilating the inorganic carbon and low molecular weight organic acids, degrading the organic pollutants and residues were summarized in this chapter.

ACS Style

Juan Wang; Huaiying Yao. Applications of DNA/RNA-stable isotope probing (SIP) in environmental microbiology. Methods in Microbiology 2021, 227 -267.

AMA Style

Juan Wang, Huaiying Yao. Applications of DNA/RNA-stable isotope probing (SIP) in environmental microbiology. Methods in Microbiology. 2021; ():227-267.

Chicago/Turabian Style

Juan Wang; Huaiying Yao. 2021. "Applications of DNA/RNA-stable isotope probing (SIP) in environmental microbiology." Methods in Microbiology , no. : 227-267.

Journal article
Published: 11 January 2021 in Agronomy
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Soil salinization typically inhibits the ability of decomposer organisms to utilize soil organic matter, and an increase in soil clay content can mediate the negative effect of salinity on carbon (C) mineralization. However, the interactive effects of soil salt concentrations and properties on C mineralization remain uncertain. In this study, a laboratory experiment was performed to investigate the interactive effects of soil salt content (0.1%, 0.3%, 0.6% and 1.0%) and texture (sandy loam, sandy clay loam and silty clay soil with 6.0%, 23.9% and 40.6% clay content, respectively) on C mineralization and microbial community composition after cotton straw addition. With increasing soil salinity, carbon dioxide (CO2) emissions from the three soils decreased, but the effect of soil salinity on the decomposition of soil organic carbon varied with soil texture. Cumulative CO2 emissions in the coarse-textured (sandy loam and sandy clay loam) soils were more affected by salinity than those in the fine-textured (silty clay) soil. This difference was probably due to the differing responses of labile and resistant organic compounds to salinity across different soil texture. Increased salinity decreased the decomposition of the stable C pool in the coarse-textured soil, by reducing the proportion of fungi to bacteria, whereas it decreased the mineralization of the active C pool in the fine-textured soil through decreasing the Gram-positive bacterial population. Overall, our results suggest that soil texture controlled the negative effect of salinity on C mineralization through regulating the soil microbial community composition.

ACS Style

Ruihuan She; Yongxiang Yu; Chaorong Ge; Huaiying Yao. Soil Texture Alters the Impact of Salinity on Carbon Mineralization. Agronomy 2021, 11, 128 .

AMA Style

Ruihuan She, Yongxiang Yu, Chaorong Ge, Huaiying Yao. Soil Texture Alters the Impact of Salinity on Carbon Mineralization. Agronomy. 2021; 11 (1):128.

Chicago/Turabian Style

Ruihuan She; Yongxiang Yu; Chaorong Ge; Huaiying Yao. 2021. "Soil Texture Alters the Impact of Salinity on Carbon Mineralization." Agronomy 11, no. 1: 128.

Original paper
Published: 08 January 2021 in Biology and Fertility of Soils
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Sorghum has a great capacity to release biological nitrification inhibitors (BNIs), but the inhibitory effect on nitrification and ammonia oxidizer populations under planted soil conditions is unclear. A pot experiment with three nitrogen (N) application rates (0, 50, and 200 mg N kg−1) was set up to detect the influence of sorghum growth on soil nitrification and investigate the function of blocking the activity of ammonia oxidizers. A 15N-labeled experiment was also conducted to detect the N form absorbed by sorghum. Sorghum root exudates were collected at 30 days after transplanting to hydroponic culture and added into cultured soil to determine the shifts in the populations of nitrifiers. The 15N labeling experiment showed that the uptake rate by sorghum of ammonium N fertilizer was 24% and that of nitrate N fertilizer was 9%, indicating that sorghum was an ammonium using plant. Compared with unplanted soil, sorghum planting had a significant inhibitory effect on the nitrification process even at the high-N fertilizer rates. Autotrophic nitrification was the prevailing process, and sorghum root exudation inhibited this process as much as dicyandiamide (DCD, 10 mg kg−1). Root exudates had a significant inhibitory effect on ammonia-oxidizing bacteria (AOB) but had no effect on ammonia-oxidizing archaea (AOA).

ACS Style

Yaying Li; Yang Zhang; Stephen James Chapman; Huaiying Yao. Biological nitrification inhibition by sorghum root exudates impacts ammonia-oxidizing bacteria but not ammonia-oxidizing archaea. Biology and Fertility of Soils 2021, 57, 399 -407.

AMA Style

Yaying Li, Yang Zhang, Stephen James Chapman, Huaiying Yao. Biological nitrification inhibition by sorghum root exudates impacts ammonia-oxidizing bacteria but not ammonia-oxidizing archaea. Biology and Fertility of Soils. 2021; 57 (3):399-407.

Chicago/Turabian Style

Yaying Li; Yang Zhang; Stephen James Chapman; Huaiying Yao. 2021. "Biological nitrification inhibition by sorghum root exudates impacts ammonia-oxidizing bacteria but not ammonia-oxidizing archaea." Biology and Fertility of Soils 57, no. 3: 399-407.

Journal article
Published: 08 January 2021 in Geoderma
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Biological nitrogen (N) fixation is one of the most significant parts of the N cycle in terrestrial ecosystems, and this process is carried out by soil diazotrophs. However, knowledge of diazotroph assembly processes and activity in response to diverse fertilization strategies in agroecosystems across a large geographical scale is still lacking. Here, we selected nine agro-ecological experimental sites that covered a wide geographical distance (~3500 km) at a continental scale, and investigated the diazotrophic communities, assembly processes, distance-decay patterns and N2 fixation activity in response to geographical factors and long-term fertilization strategies. The results showed that the dominant genera were Bradyrhizobium (~30.5%) and Azospirillum (~26.8%) in all samples, and RDA analysis showed that the relative abundance of Bradyrhizobium was positive correlated with MAP while specific negatively correlated with soil pH and the relative abundance of Azospirillum. Geographical factors (location and climate) and fertilization collectively drive diazotroph assembly processes and determine diazotroph activity. Diazotroph assembly processes were influenced by both stochastic (~36.2%) and deterministic (~63.8%) processes simultaneously at large geographical scales and under various fertilization strategies. Moreover, fertilization increased the proportion of deterministic processes under various fertilization strategies. The N fixation rate was determined by local soil properties. Fertilization changes but does not always suppress nitrogen fixation activity. Both geographical factors and fertilization through the shift of diazotroph community composition and the changes in soil properties, indirectly affected the assembly process and N fixation rate. Among soil properties, pH was the dominant factor and linearly related to diazotrophs assembly process, while N fixation rate reached peak at near-neutral pH. These results elucidate the mechanism of soil diazotroph assembly process and activity shaped by both geographical factors and fertilization; thus, expand the current understanding of the diazotroph community affected by fertilization strategies across a large geographical scale.

ACS Style

Xiangtian Meng; Hongkai Liao; Haoxin Fan; Xuechen Zhang; Yaying Li; Huaiying Yao; Bahar S. Razavi. The geographical scale dependence of diazotroph assembly and activity: Effect of a decade fertilization. Geoderma 2021, 386, 114923 .

AMA Style

Xiangtian Meng, Hongkai Liao, Haoxin Fan, Xuechen Zhang, Yaying Li, Huaiying Yao, Bahar S. Razavi. The geographical scale dependence of diazotroph assembly and activity: Effect of a decade fertilization. Geoderma. 2021; 386 ():114923.

Chicago/Turabian Style

Xiangtian Meng; Hongkai Liao; Haoxin Fan; Xuechen Zhang; Yaying Li; Huaiying Yao; Bahar S. Razavi. 2021. "The geographical scale dependence of diazotroph assembly and activity: Effect of a decade fertilization." Geoderma 386, no. : 114923.

Environmental biotechnology
Published: 02 January 2021 in Applied Microbiology and Biotechnology
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Root-associated microorganisms play an important role in plant nutrition and productivity. However, our understanding of how a plant-microbiome system responds to pre-planting soil management remains limited. Here, continuous labeling with 13CO2 gas combined with stable isotope probing (SIP) was applied to explore bacterial utilization of plant-derived carbon (C) in the tomato rhizosphere as affected by biochar amendment or reductive soil disinfestation (RSD). Our results showed that RSD treatment strongly shaped the soil bacterial community composition, while biochar soil amendment had little impact on the community in the rhizosphere of tomato. We observed that the bacterial community in the RSD treatment, which actively utilized plant-derived C, belonged to various phyla (i.e., Proteobacteria, Cyanobacteria, Verrucomicrobia, and Acidobacteria), while the genus Streptomyces (phylum Actinobacteria) was the main bacterial taxa that actively utilized plant-derived C in the biochar and control treatments. This study provides evidence that biochar application or RSD pre-planting soil management practices induced distinct bacterial utilization of plant-derived C, which may in turn regulate plant productivity in agricultural systems. KEY POINTS: • Genus Streptomyces was the main bacterial group utilizing plant-derived carbon in both control and biochar treatments. • Reductive soil disinfestation altered bacterial utilization of plant-derived carbon. • Biochar did not alter the composition of the bacterial communities but had more labeled bacterial taxa utilizing plant-derived carbon.

ACS Style

Hongkai Liao; Haoxin Fan; Yaying Li; Huaiying Yao. Influence of reductive soil disinfestation or biochar amendment on bacterial communities and their utilization of plant-derived carbon in the rhizosphere of tomato. Applied Microbiology and Biotechnology 2021, 105, 815 -825.

AMA Style

Hongkai Liao, Haoxin Fan, Yaying Li, Huaiying Yao. Influence of reductive soil disinfestation or biochar amendment on bacterial communities and their utilization of plant-derived carbon in the rhizosphere of tomato. Applied Microbiology and Biotechnology. 2021; 105 (2):815-825.

Chicago/Turabian Style

Hongkai Liao; Haoxin Fan; Yaying Li; Huaiying Yao. 2021. "Influence of reductive soil disinfestation or biochar amendment on bacterial communities and their utilization of plant-derived carbon in the rhizosphere of tomato." Applied Microbiology and Biotechnology 105, no. 2: 815-825.

Journal article
Published: 15 December 2020 in Science of The Total Environment
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Rice straw is considered as a renewable biomass energy source and its efficient utilization is still a topic worthy of attention. Black soldier fly larvae, Hermetia illucens (L.), (Diptera: Stratiomydiae) is a kind of saprophytic insect, which can effectively digest organic wastes. Here we report that alkaline peroxide-pretreatment improves the digestion of rice straw by these larvae, especially the decomposition of cellulose, which was at 70.9% compared to 58.2% without pretreatment. After conversion, the effective conversion rates of rice straw to larvae were 10.7% and 11.4%, for raw rice straw and rice straw with pretreatment, respectively. With pretreatment the composition of larval gut microorganisms was altered where Actinomyces, Dysgonomonas, Devosia and Pelagibacterium were the dominant flora for digesting rice straw. In addition, metabolism, environmental information processing and genetic information processing were the major gut microbial functions. These findings demonstrate that chemical pretreatment for the removal of lignin and hemicellulose was an effective measure for the digestion and consumption of rice straw by black soldier fly larvae.

ACS Style

Cuncheng Liu; Cunwen Wang; Huaiying Yao; Stephen J. Chapman. Pretreatment is an important method for increasing the conversion efficiency of rice straw by black soldier fly larvae based on the function of gut microorganisms. Science of The Total Environment 2020, 762, 144118 .

AMA Style

Cuncheng Liu, Cunwen Wang, Huaiying Yao, Stephen J. Chapman. Pretreatment is an important method for increasing the conversion efficiency of rice straw by black soldier fly larvae based on the function of gut microorganisms. Science of The Total Environment. 2020; 762 ():144118.

Chicago/Turabian Style

Cuncheng Liu; Cunwen Wang; Huaiying Yao; Stephen J. Chapman. 2020. "Pretreatment is an important method for increasing the conversion efficiency of rice straw by black soldier fly larvae based on the function of gut microorganisms." Science of The Total Environment 762, no. : 144118.

Journal article
Published: 11 November 2020 in Agronomy
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Although elevated CO2 (eCO2) in the atmosphere is one of the main factors influencing climate and ecosystem stability, less research on eCO2 in greenhouse soil systems has been conducted, despite their prevalence. In this article, phospholipid fatty acid (PLFA) profiling, 16S rRNA and Internally Transcribed Spacer (ITS) gene sequencing and high-throughput quantity polymerase chain reactions (HT-qPCRs) for 72 biogeochemical cycling-related genes were used to reveal the comprehensive responses of microbes to 23 days eCO2 fumigation in the soil of a tomato greenhouse. Our results indicated that eCO2 significantly increased microbial biomass (p < 0.05). The fungal community was more susceptible to eCO2 than the bacterial community; the fungal alpha diversity indices decreased significantly under eCO2 (p < 0.05) and the abundance of Ascomycota and its lower level taxa also increased significantly (p < 0.01). The absolute abundance of numerous C, N, P, S and methane cycling related genes increased significantly (p < 0.05) under eCO2. Furthermore, the microbial community structure and function were correlated with certain measured plant characteristics. Hence, the microbial ecosystem of the tomato greenhouse soil system was stimulated under eCO2. These results contribute to a greater understanding of how eCO2 in the atmosphere affects terrestrial ecosystem stability.

ACS Style

Hehua Wang; Haoxin Fan; Huaiying Yao. Effects of Elevated CO2 on Tomato (Lycopersicon esculentum Mill.) Growth and Rhizosphere Soil Microbial Community Structure and Functionality. Agronomy 2020, 10, 1752 .

AMA Style

Hehua Wang, Haoxin Fan, Huaiying Yao. Effects of Elevated CO2 on Tomato (Lycopersicon esculentum Mill.) Growth and Rhizosphere Soil Microbial Community Structure and Functionality. Agronomy. 2020; 10 (11):1752.

Chicago/Turabian Style

Hehua Wang; Haoxin Fan; Huaiying Yao. 2020. "Effects of Elevated CO2 on Tomato (Lycopersicon esculentum Mill.) Growth and Rhizosphere Soil Microbial Community Structure and Functionality." Agronomy 10, no. 11: 1752.

Environmental toxicology
Published: 26 October 2020 in Environmental Toxicology and Chemistry
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Microplastic pollution has become an increasingly pervasive issue worldwide, but little is known about its effects on the soil environment. A soil microcosm experiment is conducted using low‐density polyethylene microplastics to estimate the effect of microplastic pollution on soil nutrient cycling and the soil microbial community structure. The results showed that microplastic addition significantly promoted soil carbon dioxide emissions but not soil nitrous oxide emissions. Soil pH, dissolved organic carbon, ammonia nitrogen, the contents of total phospholipid fatty acid (PLFA), and the ratios of gram‐positive bacteria: gram‐negative bacteria and saturated: monounsaturated PLFAs significantly increased. In addition, nitrate nitrogen and the ratios of fungi: bacteria, total iso‐branched fatty acids: total anteiso‐branched fatty acids, and cyclopropyl: precursor significantly decreased with increasing microplastic addition. The addition of microplastics decreased the abundance of ammonia oxidizing bacteria and nitrite reductase (nirS) but had little effect on the functional genes of ammonia oxidizing archaear, nitrite reductase (nirK), and nitrous oxide reductase. A principal coordinate analysis of the bacterial 16S rRNA gene and fungal ITS in the microplastic addition treatments revealed that the bacterial and fungal communities formed an obvious cluster. The average abundance of some microbial species with tolerance and degradability to microplastics, such as Nocardioidaceae, Amycolatopsis, Aeromicrobium, Cytophagaceae, Betaproteobacteria, Rhodoplanes, and Mortierella, in the microplastic addition treatments was significantly higher than that of the control treatment. The results suggested that microplastics have obvious influences on microbial communities and may affect global carbon and nitrogen cycles. This article is protected by copyright. All rights reserved.

ACS Style

Bo Gao; Huaiying Yao; Yaying Li; Yizu Zhu. Microplastic Addition Alters the Microbial Community Structure and Stimulates Soil Carbon Dioxide Emissions in Vegetable‐Growing Soil. Environmental Toxicology and Chemistry 2020, 40, 352 -365.

AMA Style

Bo Gao, Huaiying Yao, Yaying Li, Yizu Zhu. Microplastic Addition Alters the Microbial Community Structure and Stimulates Soil Carbon Dioxide Emissions in Vegetable‐Growing Soil. Environmental Toxicology and Chemistry. 2020; 40 (2):352-365.

Chicago/Turabian Style

Bo Gao; Huaiying Yao; Yaying Li; Yizu Zhu. 2020. "Microplastic Addition Alters the Microbial Community Structure and Stimulates Soil Carbon Dioxide Emissions in Vegetable‐Growing Soil." Environmental Toxicology and Chemistry 40, no. 2: 352-365.

Journal article
Published: 17 September 2020 in Journal of Soils and Sediments
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This study proposed to investigate soil N transformation rates under different tea plantation ages. These plantations receive a great deal of nitrogen fertilizer each year. It was hypothesized that the inhibition of nitrification by increasing soil acidification would be counteracted by the N application. We aimed to explore whether this relationship would influence soil nitrogen transformations within tea plantations. Tea fields with different establishment periods (8 years, 36 years, and 105 years) and adjacent forest soils were collected from Hangzhou city, China. The 15N dilution technique and a 15N tracing model were used to investigate changes in soil gross N transformation rates, additionally combining the 15N dilution technique with acetylene inhibition to distinguish heterotrophic and autotrophic nitrification rates. Both 15N labeling methods demonstrated that land use conversion and fertilization significantly change the soil N transformation rates. The gross N mineralization rate was much higher in the tea soils compared with that in the adjacent forest soil and increased with planting age in the tea soils. Interestingly, appreciable NO3− production was detected in the forest soil caused by heterotrophic nitrification, whereas only a small amount of nitrate was formed by heterotrophs in the tea soils. Gross nitrification rates increased with the planting age from 8 to 36 years but decreased at 105 years. Nitrification was the main ammonium consuming process in the tea soils and a positive relationship was observed between ammonia oxidizing archaea (AOA) amoA abundance and nitrification rate, suggesting AOA as the dominant nitrification drivers in these tea soils. Overall, the conversion from forest to tea soil enhanced the gross rate of nitrification, N mineralization, and NH4+ immobilization, but N/I (nitrification/ammonium immobilization) also increased significantly in the tea plantations, indicating a high nitrate leaching and runoff risk.

ACS Style

Yingying Zhang; Jinbo Zhang; Stephen J. Chapman; Huaiying Yao; Ningguo Zheng; Christoph Müller. Tea plantation affects soil nitrogen transformations in subtropical China. Journal of Soils and Sediments 2020, 21, 441 -451.

AMA Style

Yingying Zhang, Jinbo Zhang, Stephen J. Chapman, Huaiying Yao, Ningguo Zheng, Christoph Müller. Tea plantation affects soil nitrogen transformations in subtropical China. Journal of Soils and Sediments. 2020; 21 (1):441-451.

Chicago/Turabian Style

Yingying Zhang; Jinbo Zhang; Stephen J. Chapman; Huaiying Yao; Ningguo Zheng; Christoph Müller. 2020. "Tea plantation affects soil nitrogen transformations in subtropical China." Journal of Soils and Sediments 21, no. 1: 441-451.

Journal article
Published: 16 September 2020 in CATENA
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Soil salinization largely affects greenhouse gas emissions from soils, but its effects on the temperature sensitivity (Q10) of soil carbon dioxide (CO2) emissions and nitrous oxide (N2O) emissions remain unclear. In this study, a temperature-controlled (15 °C and 25 °C) microcosm experiment was performed for 56 days to evaluate the effects of soil salinity (0.1%, 0.3%, 0.6% and 1.0% salt contents) on the Q10 of CO2 emissions, N2O emissions and phospholipid fatty acid (PLFA)-distinguishable microbial communities in sandy clay loam (SCL) soil and silty clay (SC) soil. In both soils, soil salinity inhibited soil microbial respiration under both temperature conditions. However, soil salinity significantly (p < 0.05) enhanced the temperature sensitivity of soil CO2 emissions, indicating that although soil salinization inhibited CO2 production in the soil, more CO2 would be produced in the salinized soils under the warming scenario. Increasing soil salinity simulates the release of N2O in both soils, whereas the effects of soil salinity on the Q10 of soil N2O emissions differed between the two soils. There was no significant (p > 0.05) difference in the Q10 of soil N2O emissions among the four salinity levels in the SCL soil, but soil salinization significantly (p < 0.05) increased the Q10 of the SC soil. The relative changes in soil ammonium (NH4+)-nitrogen (N) at 15–25 °C and the temperature sensitivity of Gram-positive bacteria and fungi were negatively (p < 0.05) related to the Q10 of soil CO2 emissions. The Q10 of soil N2O was positively (p < 0.05) correlated with the relative changes in soil nitrate (NO3−)-N at 15–25 °C. Overall, our results highlight the important role of soil salinity in controlling the temperature sensitivity of soil CO2 and N2O emissions, and soil salinization likely causes a high risk of soil greenhouse gas emissions under climate warming conditions.

ACS Style

Yongxiang Yu; Xing Li; Chengyi Zhao; Ningguo Zheng; Hongtao Jia; Huaiying Yao. Soil salinity changes the temperature sensitivity of soil carbon dioxide and nitrous oxide emissions. CATENA 2020, 195, 104912 .

AMA Style

Yongxiang Yu, Xing Li, Chengyi Zhao, Ningguo Zheng, Hongtao Jia, Huaiying Yao. Soil salinity changes the temperature sensitivity of soil carbon dioxide and nitrous oxide emissions. CATENA. 2020; 195 ():104912.

Chicago/Turabian Style

Yongxiang Yu; Xing Li; Chengyi Zhao; Ningguo Zheng; Hongtao Jia; Huaiying Yao. 2020. "Soil salinity changes the temperature sensitivity of soil carbon dioxide and nitrous oxide emissions." CATENA 195, no. : 104912.

Journal article
Published: 10 July 2020 in International Journal of Applied Earth Observation and Geoinformation
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Soil organic carbon (SOC) is the largest carbon pool and a key property of ecosystems. Compared with traditional field surveys, remote sensing (RS) represents a more efficient approach to mapping SOC, especially in larger-scale areas. Hyperspectral imagery provides a great potential for SOC prediction, but predicting the SOC content at a regional scale for a given year remains a great challenge. Multispectral RS images (e.g., Landsat images) with middle spatial resolution can be used as an alternative to predict SOC amongst other images. However, multispectral images are commonly affected by cloud cover and lack information on the shallow soil surface and soil surface with vegetation. By contrast, synthetic aperture radar (SAR) images can capture shallow surface information, penetrate through vegetation and are not affected by cloud cover. In this study, a new approach was evaluated for the prediction of SOC content by integrating Landsat Thematic Mapper (TM) and Advanced Land Observing Satellite (ALOS) Phased Arrayed L-band Synthetic Aperture Radar (PALSAR) images. Mainland Spain (hereafter Spain) was used as the study site. Soil samples from the Land Use and Coverage Area frame Survey (LUCAS) European topsoil dataset in 2009 were utilized to fit a random forest (RF) model of the relationships between SOC content and several covariates in Spain. SOC prediction using only the Landsat TM image and only the ALOS PALSAR image was established as benchmarks to verify the efficiency of the proposed approach. The results for Spain showed that the integrated approach holds the highest prediction accuracy, with R2, RPD and RMSE of 0.59, 1.98 and 9.27 g kg−1, respectively. The correlations between the SOC content and various covariates were investigated and discussed, and the derived indices presented high relationships with SOC. The proposed method can accurately map the SOC content covering a large area by combining spectral information on land cover from the Landsat TM and topsoil information from the ALOS PALSAR. Land covers and elevation are closely related to SOC prediction, where lands with high vegetation canopy density have high SOC content, and the SOC content in areas with slopes within 15° is mainly concentrated between 3 and 26 g kg−1.

ACS Style

Xia Wang; Yihang Zhang; Peter M. Atkinson; Huaiying Yao. Predicting soil organic carbon content in Spain by combining Landsat TM and ALOS PALSAR images. International Journal of Applied Earth Observation and Geoinformation 2020, 92, 102182 .

AMA Style

Xia Wang, Yihang Zhang, Peter M. Atkinson, Huaiying Yao. Predicting soil organic carbon content in Spain by combining Landsat TM and ALOS PALSAR images. International Journal of Applied Earth Observation and Geoinformation. 2020; 92 ():102182.

Chicago/Turabian Style

Xia Wang; Yihang Zhang; Peter M. Atkinson; Huaiying Yao. 2020. "Predicting soil organic carbon content in Spain by combining Landsat TM and ALOS PALSAR images." International Journal of Applied Earth Observation and Geoinformation 92, no. : 102182.

Journal article
Published: 24 June 2020 in Environmental Pollution
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The conversion of natural forests to tea plantations largely affects soil nitrous oxide (N2O) emissions and soil microbial communities. However, the impacts of this conversion on the contribution of fungi to N2O emission and on fungal community structure remain unclear. In this study, we determined the soil N2O emission rate, N2O production by fungi, associated fungal community diversity, and related ecological factors in chronological changes of tea crop systems (3, 36 and 105 years old tea orchards named T3, T36 and T105, respectively), and in an adjacent soil from a natural forest. The results indicate that the tea plantations significantly enhanced soil N2O production compared with the forest soil. Tea plantations significantly decreased soil pH and C/N ratio, but increased soil inorganic nitrogen (N). Furthermore, they increased the fungal contribution to the production of soil N2O, but decreased the bacterial counterpart. We also observed that fungal community and functional composition differed distinctly between tea plantations and forest. Additionally, most of the fungal groups in high N2O emission soils (T36 and T105) were identified as the genus Fusarium, which were positively correlated with soil N2O emissions. The variation in N2O emission response could be well explained by NO3−-N, soil organic carbon (SOC), C/N, and Fusarium, which contributed to up to 97% of the observed variance. Altogether, these findings provide significant direct evidence that the increase of soil N2O emissions and fungal communities be attributed to the conversion of natural forest to tea plantations.

ACS Style

Ningguo Zheng; Yongxiang Yu; Juan Wang; Stephen J. Chapman; Huaiying Yao; Yingying Zhang. The conversion of subtropical forest to tea plantation changes the fungal community and the contribution of fungi to N2O production. Environmental Pollution 2020, 265, 115106 .

AMA Style

Ningguo Zheng, Yongxiang Yu, Juan Wang, Stephen J. Chapman, Huaiying Yao, Yingying Zhang. The conversion of subtropical forest to tea plantation changes the fungal community and the contribution of fungi to N2O production. Environmental Pollution. 2020; 265 ():115106.

Chicago/Turabian Style

Ningguo Zheng; Yongxiang Yu; Juan Wang; Stephen J. Chapman; Huaiying Yao; Yingying Zhang. 2020. "The conversion of subtropical forest to tea plantation changes the fungal community and the contribution of fungi to N2O production." Environmental Pollution 265, no. : 115106.

Journal article
Published: 12 June 2020 in Environment International
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As a saprophytic insect, the black soldier fly can digest organic waste efficiently in an environmentally friendly way. However, the ability and efficiency of this insect, and the microbial mechanisms involved, in the degradation of antibiotics are largely uncharacterized. To obtain further details during the degradation of OTC (oxytetracycline) by black soldier fly larvae (larvae), the changes in intestinal bacterial communities were examined. Both ARGs (antibiotic resistance genes) and MGEs (mobile genetic elements) were found within the larval guts. At the end of the degradation period, 82.7%, 77.6% and 69.3% of OTC was degraded by larvae when the initial concentrations were 100, 1000 and 2000 mg kg−1 (dry weight), respectively, which was much higher than the degradation efficiencies (19.3–22.2%) without larvae. There was no obvious effect of OTC on the development of the larvae. Although the larval gut microorganisms were affected by OTC, they adapted to the altered environment. Enterococcus, Ignatzschineria, Providencia, Morganella, Paenalcaligenes and Actinomyces in the gut responded strongly to antibiotic exposure. Interestingly, numerous ARGs (specifically, 180 ARGs and 10 MGEs) were discovered, and significantly correlated with those of both integron-integrase gene and transposases in the larval gut. Of all the detected ARGs, tetracycline resistance genes expressed at relatively high levels and accounted for up to 67% of the total ARGs. In particular, Enterococcus, Ignatzschineria, Bordetella, Providencia and Proteus were all hosts of enzymatic modification genes of tetracycline in the guts that enabled effective degradation of OTC. These findings demonstrate that OTC can be degraded effectively and prove that the bioremediation of antibiotic contamination is enhanced by larvae. In addition, the abundance of ARGs and MGEs formed should receive attention and be considered in environmental health risk assessment systems.

ACS Style

Cuncheng Liu; Huaiying Yao; Stephen J. Chapman; Jianqiang Su; Cunwen Wang. Changes in gut bacterial communities and the incidence of antibiotic resistance genes during degradation of antibiotics by black soldier fly larvae. Environment International 2020, 142, 105834 .

AMA Style

Cuncheng Liu, Huaiying Yao, Stephen J. Chapman, Jianqiang Su, Cunwen Wang. Changes in gut bacterial communities and the incidence of antibiotic resistance genes during degradation of antibiotics by black soldier fly larvae. Environment International. 2020; 142 ():105834.

Chicago/Turabian Style

Cuncheng Liu; Huaiying Yao; Stephen J. Chapman; Jianqiang Su; Cunwen Wang. 2020. "Changes in gut bacterial communities and the incidence of antibiotic resistance genes during degradation of antibiotics by black soldier fly larvae." Environment International 142, no. : 105834.

Journal article
Published: 26 May 2020 in Applied Soil Ecology
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Increasing evidence indicates that nitrification is a vital factor in crop growth and nitrous oxide emission. Nitrification and urease inhibitors have been demonstrated to be effective in inhibiting the nitrification process and are widely used as fertilizer additives in agricultural soils. However, the effects of these inhibitors on rice N uptake and N2O production through denitrification in paddy soils remain unclear. In the present work, we compared the influences of nitrification inhibitors dicyandiamide (DCD), nitrapyrin (2-chloro-6-(trichloromethyl) pyridine; NP) and N-(n-butyl) thiophosphoric triamide (NBPT) on rice growth, the fate of urea nitrogen (N), and the abundances and activities of ammonia oxidizers and denitrifiers. The fate of urea N was determined by the 15N isotope labeling technique, and the abundances of ammonia oxidizers and denitrifiers were determined using qPCR. All three inhibitors improved rice growth mainly due to the increase in urea N use efficiency. Urea N uptake was negatively correlated with nitrification. The growth of ammonia-oxidizing bacteria (AOB), important in nitrification, was directly blocked by DCD. Additionally, NP and NBPT impeded the growth of ammonia-oxidizing archaea (AOA). In addition, NP significantly increased the microbial biomass to promote more residual urea N in soil and increased soil N transformation. NBPT significantly inhibited urea hydrolysis indirectly affecting nitrification. All three inhibitors decreased the potential denitrification rate (PDR) at the rice heading stage but had little effect on the denitrifier gene abundance except for nitrapyrin, which decreased the nirK gene abundance. DCD and NBPT may reduce the denitrification activity by decreasing the denitrification substrate (NO3−) concentration. These results suggest that DCD, NP and NBPT have a beneficial effect on improving rice N uptake and have the potential to reduce N2O generation through denitrification.

ACS Style

Xiangtian Meng; Yaying Li; Huaiying Yao; Juan Wang; Feng Dai; Yuping Wu; Stephen Chapman. Nitrification and urease inhibitors improve rice nitrogen uptake and prevent denitrification in alkaline paddy soil. Applied Soil Ecology 2020, 154, 103665 .

AMA Style

Xiangtian Meng, Yaying Li, Huaiying Yao, Juan Wang, Feng Dai, Yuping Wu, Stephen Chapman. Nitrification and urease inhibitors improve rice nitrogen uptake and prevent denitrification in alkaline paddy soil. Applied Soil Ecology. 2020; 154 ():103665.

Chicago/Turabian Style

Xiangtian Meng; Yaying Li; Huaiying Yao; Juan Wang; Feng Dai; Yuping Wu; Stephen Chapman. 2020. "Nitrification and urease inhibitors improve rice nitrogen uptake and prevent denitrification in alkaline paddy soil." Applied Soil Ecology 154, no. : 103665.