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Coffee consumption is believed to have chemopreventive and chemotherapeutic effects and to contribute to preventing the development and progression of cancer. However, there is still controversy around these claims. As indicated in our previous works, diet can influence the risk of breast cancer. Intake of coffee is hypothesized to reduce this risk, but current scientific evidence is not conclusive. This work is aimed at studying the effects of Robusta coffee bean extract on cell viability, proliferation, and apoptosis of different human cancers, especially breast cancer cell lines. To this end, cell viability was evaluated by Alamar Blue in 2D and 3D models, the cell cycle by PI, apoptosis by annexin V, mitochondrial morphology, and functionality by mitoTracker, and colony formation capacity by the clonogenic assay. Green and dark coffee extract significantly reduced viability in human breast, colorectal, brain, and bone cancer cells. Coffee anticancer activity was clearly evidenced in MDA-MB-231 (ER-) and MCF-7 (ER+) breast cancer cells but not in the normal breast cell line. In addition, coffee extract induces an increase S phase and a decrease G2/M population in breast cancer cells, affected the mitochondrial morphology, and triggered apoptosis. MDA-MB-231 breast cancer cells lost their clonogenic capacity after treatment. The antitumor activity was demonstrated in both 2D and 3D culture cell models.
Ayelén D. Nigra; Deborah De Almeida Bauer Guimarães; César G. Prucca; Otniel Freitas-Silva; Anderson J. Teodoro; Germán A. Gil. Antitumor Effects of Freeze-Dried Robusta Coffee (Coffea canephora) Extracts on Breast Cancer Cell Lines. Oxidative Medicine and Cellular Longevity 2021, 2021, 1 -16.
AMA StyleAyelén D. Nigra, Deborah De Almeida Bauer Guimarães, César G. Prucca, Otniel Freitas-Silva, Anderson J. Teodoro, Germán A. Gil. Antitumor Effects of Freeze-Dried Robusta Coffee (Coffea canephora) Extracts on Breast Cancer Cell Lines. Oxidative Medicine and Cellular Longevity. 2021; 2021 ():1-16.
Chicago/Turabian StyleAyelén D. Nigra; Deborah De Almeida Bauer Guimarães; César G. Prucca; Otniel Freitas-Silva; Anderson J. Teodoro; Germán A. Gil. 2021. "Antitumor Effects of Freeze-Dried Robusta Coffee (Coffea canephora) Extracts on Breast Cancer Cell Lines." Oxidative Medicine and Cellular Longevity 2021, no. : 1-16.
Polyphenols, condensed tannins, total flavonoids, total carotenoids, lycopene and ascorbic acid were determined besides verifying antioxidant capacity of peel, pulp and desserts (with and without sugar) of red guava (Psidium guajava L.) as well as the effects of lycopene on cytotoxicity, cell cycle and apoptosis on breast cancer cell line MCF-7. Guava peel contains 90% of the total ascorbic acid and heat treatment does not modify bioactive compounds content and antioxidant capacity. Sugar addition decreased guava pulp functional capacity. After heat treatment, lycopene content was stable, but sugar addition reduced its concentration by 57%. Lycopene (10 µM) extracted from guava and standard presented the same cytotoxic effect on MCF-7 cells. Lycopene influenced over G2-M transition check-point of the cell cycle and increased apoptotic cells percentages compared to untreated cells. The consumption of in natura guava, especially with peel can be considered an important source of bioactive compounds. Graphical Abstract
R.M. Polinati; A.J. Teodoro; M.G. Correa; F.A. Casanova; C.L.A. Passos; J.L. Silva; E. Fialho. Effects of lycopene from guava (Psidium guajava L.) derived products on breast cancer cells. Natural Product Research 2021, 1 -4.
AMA StyleR.M. Polinati, A.J. Teodoro, M.G. Correa, F.A. Casanova, C.L.A. Passos, J.L. Silva, E. Fialho. Effects of lycopene from guava (Psidium guajava L.) derived products on breast cancer cells. Natural Product Research. 2021; ():1-4.
Chicago/Turabian StyleR.M. Polinati; A.J. Teodoro; M.G. Correa; F.A. Casanova; C.L.A. Passos; J.L. Silva; E. Fialho. 2021. "Effects of lycopene from guava (Psidium guajava L.) derived products on breast cancer cells." Natural Product Research , no. : 1-4.
There is a significant indication of the beneficial health effects of fruit rich diets. Fruits of native plant species have noticeably different phytochemicals and bioactive effects. The aim of this work was to characterize and compare the constituents of jabuticaba (Myrciaria jaboticaba, MJ), jamun-berry (Syzygium cumini, SC), and malay-apple (Syzygium malaccense, SM) extracts and their influence on antioxidant activity in vitro and antiproliferative effects on human colon adenocarcinoma cells. According to the results, dried peel powders (DP) have a high anthocyanin content, phenolic compounds, and antioxidant activity when compared to freeze dried extracts (FD). M. jaboticaba dried peel powder extract had a higher total anthocyanin and phenolic compounds content (802.90 ± 1.93 and 2152.92 ± 43.95 mg/100 g, respectively). A reduction in cell viability of HT-29 cells after treatment with M. jaboticaba extracts (DP-MJ and FD-MJ) was observed via MTT assay. Flow cytometry showed that the treatment with the anthocyanin-rich extracts from MJ, SC, and SM had an inhibitory impact on cell development due to G2/M arrest and caused a rise in apoptotic cells in relation to the control group. The findings of this study highlight the potential of peel powders from Myrtaceae fruits as an important source of natural antioxidants and a protective effect against colon adenocarcinoma.
Nayara Simas Frauches; Júlia Montenegro; Thuane Amaral; Joel Abreu; Gabriela Laiber; Jorge Junior; Renata Borguini; Manuela Santiago; Sidney Pacheco; Vania Nakajima; Ronoel Godoy; Anderson Teodoro. Antiproliferative Activity on Human Colon Adenocarcinoma Cells and In Vitro Antioxidant Effect of Anthocyanin-Rich Extracts from Peels of Species of the Myrtaceae Family. Molecules 2021, 26, 564 .
AMA StyleNayara Simas Frauches, Júlia Montenegro, Thuane Amaral, Joel Abreu, Gabriela Laiber, Jorge Junior, Renata Borguini, Manuela Santiago, Sidney Pacheco, Vania Nakajima, Ronoel Godoy, Anderson Teodoro. Antiproliferative Activity on Human Colon Adenocarcinoma Cells and In Vitro Antioxidant Effect of Anthocyanin-Rich Extracts from Peels of Species of the Myrtaceae Family. Molecules. 2021; 26 (3):564.
Chicago/Turabian StyleNayara Simas Frauches; Júlia Montenegro; Thuane Amaral; Joel Abreu; Gabriela Laiber; Jorge Junior; Renata Borguini; Manuela Santiago; Sidney Pacheco; Vania Nakajima; Ronoel Godoy; Anderson Teodoro. 2021. "Antiproliferative Activity on Human Colon Adenocarcinoma Cells and In Vitro Antioxidant Effect of Anthocyanin-Rich Extracts from Peels of Species of the Myrtaceae Family." Molecules 26, no. 3: 564.
Amazonian fruits are excellent sources of bioactive compounds and can be used in beverages to improve the nutritional and sensorial characteristics. The present study aimed to develop a blend of murici (Byrsonima Crassifolia (L.) Kunth) and taperebá (Spondias Mombin L.) through experimental design and investigating the nutritional and sensorial characteristics of fruits and beverages. The murici was highlighted as higher vitamin C content (58.88 mg · 100 g−1) compared to taperebá (25.93 mg · 100 g−1). The murici and taperebá are good sources of total phenolic compounds (taperebá 1304.15 ± 19.14 mgGAE · 100 g−1 and the murici of 307.52 ± 19.73 mg GAE · 100 g−1) and flavonoids (174.87 ± 1.76 μgQE/g and 129.46 ± 10.68 μgQE/g, murici and taperebá, respectively), when compared to other Brazilian fruits. The antioxidant capacity in different methods revealed that the taperebá had a higher average in the results, only in the ORAC method and did not present a significant difference (p > 0.05) in relation to the murici. The beverage development was performed using experimental design 23, showed through sensory analysis and surface response methodology that murici and high sugar content (between 12.5 and 14.2% of sugar) influenced in sensory acceptance. Our findings indicate that beverages with improved nutrition and a sensory acceptance can be prepared using taperebá and murici fruits.
Adriana Aniceto; Julia Montenegro; Rafael Da Silva Cadena; Anderson Junger Teodoro. Physicochemical Characterization, Antioxidant Capacity, and Sensory Properties of Murici (Byrsonimacrassifolia (L.) Kunth) and Taperebá (Spondiasmombin L.) Beverages. Molecules 2021, 26, 332 .
AMA StyleAdriana Aniceto, Julia Montenegro, Rafael Da Silva Cadena, Anderson Junger Teodoro. Physicochemical Characterization, Antioxidant Capacity, and Sensory Properties of Murici (Byrsonimacrassifolia (L.) Kunth) and Taperebá (Spondiasmombin L.) Beverages. Molecules. 2021; 26 (2):332.
Chicago/Turabian StyleAdriana Aniceto; Julia Montenegro; Rafael Da Silva Cadena; Anderson Junger Teodoro. 2021. "Physicochemical Characterization, Antioxidant Capacity, and Sensory Properties of Murici (Byrsonimacrassifolia (L.) Kunth) and Taperebá (Spondiasmombin L.) Beverages." Molecules 26, no. 2: 332.
Coffee consumption has been investigated as a protective factor against prostate cancer. Coffee may be related to prostate cancer risk reduction due to its phytochemical compounds, such as caffeine, chlorogenic acids, and trigonelline. The roasting process affects the content of the phytochemicals and undesired compounds can be formed. Microwave-assisted extraction is an alternative to conventional extraction techniques since it preserves more bioactive compounds. Therefore, this study aimed to evaluate the phytochemical composition and the putative preventive effects in prostate cancer development of coffee beans submitted to four different coffee-roasting degrees extracted using microwave-assisted extraction. Coffea arabica green beans (1) were roasted into light (2), medium (3) and dark (4) and these four coffee samples were submitted to microwave-assisted extraction. The antioxidant capacity of these samples was evaluated by five different methods. Caffeine, chlorogenic acid and caffeic acid were measured through HPLC. Samples were tested against PC-3 and DU-145 metastatic prostate cancer cell lines regarding their effects on cell viability, cell cycle progression and apoptotic cell death. We found that green and light roasted coffee extracts had the highest antioxidant activity. Caffeine content was not affected by roasting, chlorogenic acid was degraded due to the temperature, and caffeic acid increased in light roasted and decreased in medium and dark roasted. Green and light roasted coffee extracts promoted higher inhibition of cell viability, caused greater cell cycle arrest in S and G2/M and induced apoptosis more compared to medium and dark roasted coffee extracts and the control samples. Coffee extracts were more effective against DU-145 than in PC-3 cells. Our data provide initial evidence that among the four tested samples, the consumption of green and light coffee extracts contributes to inhibit prostate cancer tumor progression features, potentially preventing aspects related to advanced prostate cancer subtypes.
Júlia Montenegro; Lauriza Silva dos Santos; Rodrigo Gonçalves Gusmão de Souza; Larissa Gabrielly Barbosa Lima; Daniella Santos Mattos; Bruna Prunes Pena Baroni Viana; Ana Clara Santos Da Fonseca Bastos; Leda Muzzi; Carlos Adam Conte-Júnior; Etel Rodrigues Pereira Gimba; Otniel Freitas-Silva; Anderson Junger Teodoro. Bioactive compounds, antioxidant activity and antiproliferative effects in prostate cancer cells of green and roasted coffee extracts obtained by microwave-assisted extraction (MAE). Food Research International 2020, 140, 110014 .
AMA StyleJúlia Montenegro, Lauriza Silva dos Santos, Rodrigo Gonçalves Gusmão de Souza, Larissa Gabrielly Barbosa Lima, Daniella Santos Mattos, Bruna Prunes Pena Baroni Viana, Ana Clara Santos Da Fonseca Bastos, Leda Muzzi, Carlos Adam Conte-Júnior, Etel Rodrigues Pereira Gimba, Otniel Freitas-Silva, Anderson Junger Teodoro. Bioactive compounds, antioxidant activity and antiproliferative effects in prostate cancer cells of green and roasted coffee extracts obtained by microwave-assisted extraction (MAE). Food Research International. 2020; 140 ():110014.
Chicago/Turabian StyleJúlia Montenegro; Lauriza Silva dos Santos; Rodrigo Gonçalves Gusmão de Souza; Larissa Gabrielly Barbosa Lima; Daniella Santos Mattos; Bruna Prunes Pena Baroni Viana; Ana Clara Santos Da Fonseca Bastos; Leda Muzzi; Carlos Adam Conte-Júnior; Etel Rodrigues Pereira Gimba; Otniel Freitas-Silva; Anderson Junger Teodoro. 2020. "Bioactive compounds, antioxidant activity and antiproliferative effects in prostate cancer cells of green and roasted coffee extracts obtained by microwave-assisted extraction (MAE)." Food Research International 140, no. : 110014.
Coffee, besides being one of the most consumed stimulating beverages in the world, has important bioactive activities, which have been attracting increasing attention from researchers.
Laurent Dos Santos De Souza; Isabella Porto Carrero Horta; Lana De Souza Rosa; Larissa Gabrielly Barbosa Lima; Jeane Santos da Rosa; Julia Montenegro; Lauriza Da Silva Santos; Raquel Bernardo Nana de Castro; Otniel Freitas-Silva; Anderson Junger Teodoro. Effect of the roasting levels of Coffea arabica L. extracts on their potential antioxidant capacity and antiproliferative activity in human prostate cancer cells. RSC Advances 2020, 10, 30115 -30126.
AMA StyleLaurent Dos Santos De Souza, Isabella Porto Carrero Horta, Lana De Souza Rosa, Larissa Gabrielly Barbosa Lima, Jeane Santos da Rosa, Julia Montenegro, Lauriza Da Silva Santos, Raquel Bernardo Nana de Castro, Otniel Freitas-Silva, Anderson Junger Teodoro. Effect of the roasting levels of Coffea arabica L. extracts on their potential antioxidant capacity and antiproliferative activity in human prostate cancer cells. RSC Advances. 2020; 10 (50):30115-30126.
Chicago/Turabian StyleLaurent Dos Santos De Souza; Isabella Porto Carrero Horta; Lana De Souza Rosa; Larissa Gabrielly Barbosa Lima; Jeane Santos da Rosa; Julia Montenegro; Lauriza Da Silva Santos; Raquel Bernardo Nana de Castro; Otniel Freitas-Silva; Anderson Junger Teodoro. 2020. "Effect of the roasting levels of Coffea arabica L. extracts on their potential antioxidant capacity and antiproliferative activity in human prostate cancer cells." RSC Advances 10, no. 50: 30115-30126.
The present study aimed to evaluate the antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cancer cell lines (PC-3 and DU-145). Five different whey beverages were manufactured: conventional whey beverage (without addition of probiotic strain, CTL); and whey beverages containing Lactobacillus acidophilus La-05, Lactobacillus acidophilus La-03, Lactobacillus casei-01, and Bifidobacterium animalis Bb-12. Cell viability was determined by MTT assay and cell cycle arrest, and apoptosis by flow cytometry. All the samples presented cytotoxic activities against both cell lines. A decrease in the percentage of PC-3 cells in G0/G1 and S, followed by an increase in G2/M phase were observed with L. casei-01, Bb-12 and La-05 beverages (50.0 and 100.0 µg/mL). The extracts of the whey beverages caused extensive apoptosis induction in both cells’ lines, regardless of the probiotic strain. However, the whey beverage added with L. casei-01 might be a better candidate against prostate cancer cells.
Lana S. Rosa; Mariana L. Santos; Joel P. Abreu; Celso F. Balthazar; Ramon S. Rocha; Hugo L.A. Silva; Erick A. Esmerino; Maria Carmela K.H. Duarte; Tatiana Colombo Pimentel; Mônica Q. Freitas; Márcia C. Silva; Adriano G. Cruz; Anderson J. Teodoro. Antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cell lines. Food Research International 2020, 137, 109450 .
AMA StyleLana S. Rosa, Mariana L. Santos, Joel P. Abreu, Celso F. Balthazar, Ramon S. Rocha, Hugo L.A. Silva, Erick A. Esmerino, Maria Carmela K.H. Duarte, Tatiana Colombo Pimentel, Mônica Q. Freitas, Márcia C. Silva, Adriano G. Cruz, Anderson J. Teodoro. Antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cell lines. Food Research International. 2020; 137 ():109450.
Chicago/Turabian StyleLana S. Rosa; Mariana L. Santos; Joel P. Abreu; Celso F. Balthazar; Ramon S. Rocha; Hugo L.A. Silva; Erick A. Esmerino; Maria Carmela K.H. Duarte; Tatiana Colombo Pimentel; Mônica Q. Freitas; Márcia C. Silva; Adriano G. Cruz; Anderson J. Teodoro. 2020. "Antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cell lines." Food Research International 137, no. : 109450.
The objective was to investigate the role of consumption of grape juice (GJ), red wine (RW) or resveratrol solution (RS) on rats treated with a high-fat diet (HFD). Among the drinks offered, GJ had lower content of polyphenols and trans-resveratrol. Nevertheless, GJ showed similar content of anthocyanin and antioxidant activity to RW, although higher than RS. In rats treated with HFD, consumption of GJ presented best antioxidant and anti-inflammatory activity, reducing glutathione peroxidase and interleukin-6 serum levels. In addition, GJ promoted better levels of cholesterol and liver markers. On the other hand, RW aggravated the oxidizing effect of HFD, increasing catalase activity and interleukin-6 level. Already, RS showed no benefit in animals. Thus, GJ minimized the effects of HFD on oxidative stress and inflammation beyond promoted better levels of lipid profile and liver biomarkers. However, consumption of RS showed no benefit and RW revealed a pro-oxidant effect, as did HFD.
Teresa Palmisciano Bedê; Vanessa De Jesus; Vanessa Rosse De Souza; Vânia Mattoso; Joel Pimentel Abreu; Juliana Dias; Anderson Junger Teodoro; Vilma Blondet De Azeredo. Effect of grape juice, red wine and resveratrol solution on antioxidant, anti-inflammatory, hepactic function and lipid profile in rats feds with high-fat diet. Natural Product Research 2020, 1 -6.
AMA StyleTeresa Palmisciano Bedê, Vanessa De Jesus, Vanessa Rosse De Souza, Vânia Mattoso, Joel Pimentel Abreu, Juliana Dias, Anderson Junger Teodoro, Vilma Blondet De Azeredo. Effect of grape juice, red wine and resveratrol solution on antioxidant, anti-inflammatory, hepactic function and lipid profile in rats feds with high-fat diet. Natural Product Research. 2020; ():1-6.
Chicago/Turabian StyleTeresa Palmisciano Bedê; Vanessa De Jesus; Vanessa Rosse De Souza; Vânia Mattoso; Joel Pimentel Abreu; Juliana Dias; Anderson Junger Teodoro; Vilma Blondet De Azeredo. 2020. "Effect of grape juice, red wine and resveratrol solution on antioxidant, anti-inflammatory, hepactic function and lipid profile in rats feds with high-fat diet." Natural Product Research , no. : 1-6.
Increased fruit consumption due its protective effect on the organism is accompanied by the development of the processing industry of these products. The aim of this work was to optimize fruit pulp‐based beverage formulations from the murici and tapereba Amazon region, taking into account their sensory acceptance and antioxidant activity. Total soluble solid content, reducing sugar content, titratable acidity contents, pH, and ascorbic acid content were determined in pulps and formulations. The total content phenolic compounds and antioxidant activity were also evaluated. A 22 factorial experiment was formulated to optimize ingredients for the production of murici and tapereba fruit drinks. The murici pulp had higher acidity and higher ascorbic acid content. The analysis of phenolic compounds and antioxidant activity presented higher quantity in tapereba pulp. Tapereba‐based beverages had better acceptance by the evaluated criteria. Fruit‐based beverages murici and tapereba are a well‐accepted product and have important nutritional characteristics.
Vanessa Rosse De Souza; Adriana Aniceto; Joel Pimentel de Abreu; Julia Montenegro; Bruno Boquimpani; Vanessa Azevedo De Jesuz; Monique De Barros Elias Campos; Paulo Sérgio Marcellini; Otniel Freitas‐Silva; Rafael Cadena; Anderson Junger Teodoro. Fruit‐based drink sensory, physicochemical, and antioxidant properties in the Amazon region: Murici ( Byrsonima crassifolia (L.) Kunth and verbascifolia (L.) DC) and tapereba ( Spondia mombin ). Food Science & Nutrition 2020, 8, 2341 -2347.
AMA StyleVanessa Rosse De Souza, Adriana Aniceto, Joel Pimentel de Abreu, Julia Montenegro, Bruno Boquimpani, Vanessa Azevedo De Jesuz, Monique De Barros Elias Campos, Paulo Sérgio Marcellini, Otniel Freitas‐Silva, Rafael Cadena, Anderson Junger Teodoro. Fruit‐based drink sensory, physicochemical, and antioxidant properties in the Amazon region: Murici ( Byrsonima crassifolia (L.) Kunth and verbascifolia (L.) DC) and tapereba ( Spondia mombin ). Food Science & Nutrition. 2020; 8 (5):2341-2347.
Chicago/Turabian StyleVanessa Rosse De Souza; Adriana Aniceto; Joel Pimentel de Abreu; Julia Montenegro; Bruno Boquimpani; Vanessa Azevedo De Jesuz; Monique De Barros Elias Campos; Paulo Sérgio Marcellini; Otniel Freitas‐Silva; Rafael Cadena; Anderson Junger Teodoro. 2020. "Fruit‐based drink sensory, physicochemical, and antioxidant properties in the Amazon region: Murici ( Byrsonima crassifolia (L.) Kunth and verbascifolia (L.) DC) and tapereba ( Spondia mombin )." Food Science & Nutrition 8, no. 5: 2341-2347.
Rafael Gonçalves; Anderson Teodoro. O uso de biomoléculas contra infecções virais. ?!Pesquisar é preciso?! 2020, 1 .
AMA StyleRafael Gonçalves, Anderson Teodoro. O uso de biomoléculas contra infecções virais. ?!Pesquisar é preciso?!. 2020; ():1.
Chicago/Turabian StyleRafael Gonçalves; Anderson Teodoro. 2020. "O uso de biomoléculas contra infecções virais." ?!Pesquisar é preciso?! , no. : 1.
The metabolite profiling associated with the antioxidant potential of Amazonian fruits represents an important step to the bioactive compound′s characterization due to the large biodiversity in this region. The comprehensive bioactive compounds profile and antioxidant capacities of mamey apple (Mammea americana), camapu (Physalis angulata), and uxi (Endopleura uchi) was determined for the first time. Bioactive compounds were characterized by ultra-performance liquid chromatography coupled to high resolution mass spectrometry (UPLC-MSE) in aqueous and ethanolic extracts. Globally, a total of 293 metabolites were tentatively identified in mamey apple, campau, and uxi extracts. The main classes of compounds in the three species were terpenoids (61), phenolic acids (58), and flavonoids (53). Ethanolic extracts of fruits showed higher antioxidant activity and total ion abundance of bioactive compounds than aqueous. Uxi had the highest values of phenolic content (701.84 mg GAE/100 g), ABTS (1602.7 μmol Trolox g−1), and ORAC (15.04 μmol Trolox g−1). Mamey apple had the highest results for DPPH (1168.42 μmol TE g−1) and FRAP (1381.13 μmol FSE g−1). Nuclear magnetic resonance (NMR) spectroscopy results showed that sugars and lipids were the substances with the highest amounts in mamey apple and camapu. Data referring to chemical characteristics and antioxidant capacity of these fruits can contribute to their economic exploitation.
Larissa Gabrielly Barbosa Lima; Julia Montenegro; Joel Pimentel De Abreu; Millena Cristina Barros Santos; Talita Nascimento; Maiara Da Silva Santos; Antônio Gilberto Ferreira; Luiz Claudio Cameron; Mariana Simões Larraz Ferreira; Anderson Junger Teodoro. Metabolite Profiling by UPLC-MSE, NMR, and Antioxidant Properties of Amazonian Fruits: Mamey Apple (Mammea Americana), Camapu (Physalis Angulata), and Uxi (Endopleura Uchi). Molecules 2020, 25, 342 .
AMA StyleLarissa Gabrielly Barbosa Lima, Julia Montenegro, Joel Pimentel De Abreu, Millena Cristina Barros Santos, Talita Nascimento, Maiara Da Silva Santos, Antônio Gilberto Ferreira, Luiz Claudio Cameron, Mariana Simões Larraz Ferreira, Anderson Junger Teodoro. Metabolite Profiling by UPLC-MSE, NMR, and Antioxidant Properties of Amazonian Fruits: Mamey Apple (Mammea Americana), Camapu (Physalis Angulata), and Uxi (Endopleura Uchi). Molecules. 2020; 25 (2):342.
Chicago/Turabian StyleLarissa Gabrielly Barbosa Lima; Julia Montenegro; Joel Pimentel De Abreu; Millena Cristina Barros Santos; Talita Nascimento; Maiara Da Silva Santos; Antônio Gilberto Ferreira; Luiz Claudio Cameron; Mariana Simões Larraz Ferreira; Anderson Junger Teodoro. 2020. "Metabolite Profiling by UPLC-MSE, NMR, and Antioxidant Properties of Amazonian Fruits: Mamey Apple (Mammea Americana), Camapu (Physalis Angulata), and Uxi (Endopleura Uchi)." Molecules 25, no. 2: 342.
This work evaluated the effect of grape juice, red wine and resveratrol in liver parameters of rats submitted to high-fat diet. Experimental model was conducted with groups of adult females Rattus norvegicus: control (CG); high-fat (HG); grape juice (JG); red wine (RW) and resveratrol solution (RG). The high-fat diet significantly altered hepatocytes and Kupffer cells in all treated groups. HG group presented severe steatosis followed hepatocyte ballooning and tissue damages. JG group minimized hepatic histological lesion caused by high-fat diet and WG group also induced steatosis and inflammation in hepatocytes, similar to HG. Still, resveratrol protected the tissue against fatty liver disease by reducing fat infiltration and inflammation, indicating possible therapeutic effects on the liver. Cell cycle analysis showed that HG promoted damage to the tissue, reducing the viable cell content and increasing apoptosis, even when associated with wine consumption or isolated resveratrol. However, JG protected the liver against cell damage generated by the diet. Consumption of grape juice, even associated with a high-fat diet, represents a promising protection of the liver against cellular damage, but red wine further affects the tissue, and resveratrol alone was able to reduce damage but did not minimize cellular damage to the liver.
Teresa P. Bedê; Vanessa A. Jesuz; Vanessa R. Souza; Monique B. Elias; Felipe L. Oliveira; Juliana F. Dias; Anderson J. Teodoro; Vilma B. Azeredo. Effects of grape juice, red wine and resveratrol on liver parameters of rat submitted high-fat diet. Anais da Academia Brasileira de Ciências 2020, 92, 1 .
AMA StyleTeresa P. Bedê, Vanessa A. Jesuz, Vanessa R. Souza, Monique B. Elias, Felipe L. Oliveira, Juliana F. Dias, Anderson J. Teodoro, Vilma B. Azeredo. Effects of grape juice, red wine and resveratrol on liver parameters of rat submitted high-fat diet. Anais da Academia Brasileira de Ciências. 2020; 92 (2):1.
Chicago/Turabian StyleTeresa P. Bedê; Vanessa A. Jesuz; Vanessa R. Souza; Monique B. Elias; Felipe L. Oliveira; Juliana F. Dias; Anderson J. Teodoro; Vilma B. Azeredo. 2020. "Effects of grape juice, red wine and resveratrol on liver parameters of rat submitted high-fat diet." Anais da Academia Brasileira de Ciências 92, no. 2: 1.
Breast cancer is the most frequent and lethal neoplastic disease among women worldwide. Psidium Guajava is a promising functional food against cancer, owing to a variety of bioactive compounds. This study aimed to evaluate the anticarcinogenic potential of Pedro Sato (PS), Hitigio (HI) and Tsumori (TS) guava cultivars fruit pulp extracts in MDA-MB-435 and MCF-7 human breast cancer cells. The antioxidant capacity of the extracts and their effect on cell viability, cell cycle and apoptosis were assessed. Additionally, the concentration of carotenoids, total phenolics, ascorbic acid and other physicochemical parameters were evaluated. PS pulp extract showed the highest in vitro antioxidative activity by all tested methods, as well as the highest content of lycopene and total phenolics, while TS pulp extract presented the highest concentration of β-carotene. After 48 hours treatment, all guava cultivars’ extracts caused reduction of MDA-MB-435 and MCF-7 cells viability, with PS and HI being the most effective extracts. All guava extracts caused MDA-MB-435 and MCF-7 cell count reduction in G0/G1 and G2/M phases and increased apoptosis. The present results strongly suggest that guava pulp exerts antiproliferative effect on breast adenocarcinoma cells.
Mariana G. Correa; Jessica S. Couto; Bruno B. Trindade; Joel P. Abreu; Vânia Nakajima; Felipe L. Oliveira; Adriana Farah; Anderson J. Teodoro. Antiproliferative effect of guava fruit extracts in MDA-MB-435 and MCF-7 human breast cancer cell lines. Anais da Academia Brasileira de Ciências 2020, 92, 1 .
AMA StyleMariana G. Correa, Jessica S. Couto, Bruno B. Trindade, Joel P. Abreu, Vânia Nakajima, Felipe L. Oliveira, Adriana Farah, Anderson J. Teodoro. Antiproliferative effect of guava fruit extracts in MDA-MB-435 and MCF-7 human breast cancer cell lines. Anais da Academia Brasileira de Ciências. 2020; 92 (2):1.
Chicago/Turabian StyleMariana G. Correa; Jessica S. Couto; Bruno B. Trindade; Joel P. Abreu; Vânia Nakajima; Felipe L. Oliveira; Adriana Farah; Anderson J. Teodoro. 2020. "Antiproliferative effect of guava fruit extracts in MDA-MB-435 and MCF-7 human breast cancer cell lines." Anais da Academia Brasileira de Ciências 92, no. 2: 1.
BACKGROUND This study evaluated the impact of cooking on the profile of bioactive compounds in unripe breadfruit. To this end, the accessibility of bioactive compounds by various solvents was assessed through total phenolic content (TPC) and antioxidant capacity analyses. The most efficient solvent was applied to extract the metabolites that were evaluated by ultra‐performance liquid chromatography coupled with high‐resolution quadrupole time‐of‐flight mass spectrometry in MSE mode (UPLC‐QTOF‐MSE). RESULTS Cooked and raw breadfruit presented similar TPC and antioxidant capacities in almost all extracts and pure water proved to be the best extractor. Globally, 146 bioactive compounds have been identified for both raw and cooked fruits aqueous extracts. Most of these compounds were stable to the heat treatment applied (121 °C/ 10 minutes). However, results revealed that 22 metabolites contributed to significantly distinguishing the raw from the cooked samples. Among those, 15 compounds such as pyrogallol, 1‐acetoxypinoresinol, and scopolin evidenced higher relative abundance in the cooked extracts. On the other hand, only seven metabolites, such as 4‐hydroxyhippuric acid, epicatechin, and leptodactylone decreased post‐heating. CONCLUSIONS Cooking promoted little alteration in the bioactive compounds profile of immature breadfruit and thus appears to be an exploitation alternative for this perishable fruit, which seems to be a source of a large range of bioactive compounds. This article is protected by copyright. All rights reserved.
Talita Nascimento; Millena Cristina Barros Santos; Joel Pimentel de Abreu; Iris Lengruber Gonçalves Teixeira De Almeida; Márcia Barreto Da Silva Feijó; Anderson Teodoro; Mariana Simões Larraz Ferreira; Luiz Claudio Cameron; Maria Gabriela Bello Koblitz. Effects of cooking on the phytochemical profile of breadfruit as revealed by high‐resolution UPLC–MS E. Journal of the Science of Food and Agriculture 2019, 100, 1962 -1970.
AMA StyleTalita Nascimento, Millena Cristina Barros Santos, Joel Pimentel de Abreu, Iris Lengruber Gonçalves Teixeira De Almeida, Márcia Barreto Da Silva Feijó, Anderson Teodoro, Mariana Simões Larraz Ferreira, Luiz Claudio Cameron, Maria Gabriela Bello Koblitz. Effects of cooking on the phytochemical profile of breadfruit as revealed by high‐resolution UPLC–MS E. Journal of the Science of Food and Agriculture. 2019; 100 (5):1962-1970.
Chicago/Turabian StyleTalita Nascimento; Millena Cristina Barros Santos; Joel Pimentel de Abreu; Iris Lengruber Gonçalves Teixeira De Almeida; Márcia Barreto Da Silva Feijó; Anderson Teodoro; Mariana Simões Larraz Ferreira; Luiz Claudio Cameron; Maria Gabriela Bello Koblitz. 2019. "Effects of cooking on the phytochemical profile of breadfruit as revealed by high‐resolution UPLC–MS E." Journal of the Science of Food and Agriculture 100, no. 5: 1962-1970.
Murici (Byrsonima crassifolia (L.) Kunth and B. verbascifolia (L.) DC.) and tapereba (Spondias mombin) are Amazonian fruits that contain bioactive compounds. Biochemical and molecular characterization of these fruits can reveal their potential use in preventing diseases, including cancer. The extracts were characterized regarding the presence and profile of carotenoids by high-performance liquid chromatography (HPLC), total phenolic content by the Folin-Ciocalteu assay, and antioxidant activity by antioxidant value 2,2-diphenyl-1-picrylhydrazyl (DPPH) content analysis, 22,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) content analysis, Ferric-Reducing Ability of Plasma (FRAP), and Oxygen Radical Absorbance Capacity (ORAC) analysis. The extracts of tapereba and murici studied were important sources of total carotenoids and lutein, respectively. The extracts were then tested for their effect on the viability of the A2780 ovarian cancer (OC) cell line and its cisplatin (CDDP)-resistant derived cell line, called ACRP, by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. Their influence on cell cycle and apoptosis were analyzed by using flow cytometry. Murici and tapereba cell extracts exhibited a strong bioactivity by inhibiting A2780 and ACRP cell viability by 76.37% and 78.37%, respectively, besides modulating the cell cycle and inducing apoptotic cell death. Our results open new perspectives for the development of innovative therapeutic strategies using these Amazon fruit extracts to sensitize ovarian cancer cells to current chemotherapeutic options.
Vanessa Rosse De Souza; Mariana Concentino Menezes Brum; Isabella Dos Santos Guimarães; Paula De Freitas Dos Santos; Thuane Oliveira Do Amaral; Joel Pimentel Abreu; Thuane Passos; Otniel Freitas-Silva; Etel Rodrigues Pereira Gimba; Anderson Junger Teodoro. Amazon Fruits Inhibit Growth and Promote Pro-apoptotic Effects on Human Ovarian Carcinoma Cell Lines. Biomolecules 2019, 9, 707 .
AMA StyleVanessa Rosse De Souza, Mariana Concentino Menezes Brum, Isabella Dos Santos Guimarães, Paula De Freitas Dos Santos, Thuane Oliveira Do Amaral, Joel Pimentel Abreu, Thuane Passos, Otniel Freitas-Silva, Etel Rodrigues Pereira Gimba, Anderson Junger Teodoro. Amazon Fruits Inhibit Growth and Promote Pro-apoptotic Effects on Human Ovarian Carcinoma Cell Lines. Biomolecules. 2019; 9 (11):707.
Chicago/Turabian StyleVanessa Rosse De Souza; Mariana Concentino Menezes Brum; Isabella Dos Santos Guimarães; Paula De Freitas Dos Santos; Thuane Oliveira Do Amaral; Joel Pimentel Abreu; Thuane Passos; Otniel Freitas-Silva; Etel Rodrigues Pereira Gimba; Anderson Junger Teodoro. 2019. "Amazon Fruits Inhibit Growth and Promote Pro-apoptotic Effects on Human Ovarian Carcinoma Cell Lines." Biomolecules 9, no. 11: 707.
This study evaluated the effects of tomato sauce and lycopene on hepatic and cardiac cell biomarkers in rats fed a high-fat diet. Animals were split into five groups: control group, high-fat group (HG), high-fat tomato sauce group, high-fat lycopene 2 mg, and high-fat lycopene 4 mg. Food and water were offered ad libitum, whereas tomato sauce and lycopene (2 and 4 mg/day) were offered daily for 60 days. Body, heart, and liver weights, cardiosomatic and hepatosomatic indices, and serum parameters were also analyzed in rats. The animals' hearts and liver were processed, and cells were examined by flow cytometry. Results showed that the groups receiving tomato sauce and lycopene had lower glycemia. The serum concentration of high-density lipoprotein cholesterol, hepatic enzymes, and tumor necrosis factor-α did not change upon treatment. Tomato sauce and lycopene supplementation did not increase interleukin-1β in response to a high-fat diet. Cell cycle analysis of cardiac and liver cells showed a lower percentage of cells in the G0/G1 phase and an increase in the G2/M phase in HG. Both lycopene and tomato sauce reversed this effect. Both lycopene and tomato sauce reversed this effect and prevented high-fat diet-stimulated cardiac and liver cell death. Supplementation of tomato sauce and lycopene showed beneficial effects on cardiac and liver cell metabolism; therefore, it is suggested as a nutritional approach for the prevention and treatment of cardiovascular diseases and nonalcoholic hepatic steatosis.
Vanessa Azevedo De Jesuz; Monique De Barros Elias Campos; Vanessa Rosse De Souza; Teresa Palmiciano Bede; Bianca Portugal Tavares De Moraes; Adriana Ribeiro Silva; Cassiano Felippe Gonçalves De Albuquerque; Vilma Blondet De Azeredo; Anderson Teodoro. Lycopene and Tomato Sauce Improve Hepatic and Cardiac Cell Biomarkers in Rats. Journal of Medicinal Food 2019, 22, 1175 -1182.
AMA StyleVanessa Azevedo De Jesuz, Monique De Barros Elias Campos, Vanessa Rosse De Souza, Teresa Palmiciano Bede, Bianca Portugal Tavares De Moraes, Adriana Ribeiro Silva, Cassiano Felippe Gonçalves De Albuquerque, Vilma Blondet De Azeredo, Anderson Teodoro. Lycopene and Tomato Sauce Improve Hepatic and Cardiac Cell Biomarkers in Rats. Journal of Medicinal Food. 2019; 22 (11):1175-1182.
Chicago/Turabian StyleVanessa Azevedo De Jesuz; Monique De Barros Elias Campos; Vanessa Rosse De Souza; Teresa Palmiciano Bede; Bianca Portugal Tavares De Moraes; Adriana Ribeiro Silva; Cassiano Felippe Gonçalves De Albuquerque; Vilma Blondet De Azeredo; Anderson Teodoro. 2019. "Lycopene and Tomato Sauce Improve Hepatic and Cardiac Cell Biomarkers in Rats." Journal of Medicinal Food 22, no. 11: 1175-1182.
Lycopene is more bioavailable in processed tomato products than in raw tomatoes, since arrangement of cis-isomers of lycopene during food processing and storage may increase its biological activity. The aim of the study is evaluate the influence of lycopene content from different tomato-based food products (extract, paste, ketchup and sauce) on cell proliferation, cell cycle, and rate of apoptosis of human prostate cancer cell lines. DU-145 and PC-3 cell lines were treated with lycopene content from different tomato-based food products (500-5000 μg/mL) for 96 h. The data showed a decrease in cell viability in both DU-145 and PC-3 cells after treatment with all lycopene extracts from tomato-based food products. Analysis of cell cycle revealed a decrease in the percentage of prostate cancer cells in G0/G1 and G2/M phases after 96 h of treatment when using lycopene content from tomato paste and tomato extract. However, lycopene extracted from tomato sauce and ketchup promoted a decrease in the percentage of cells in G0/G1 phase and an increase in S and G2/M phases after 96 h of treatment. Lycopene content from all of those tomato-based food products also increased apoptosis in both prostate cancer cell lines. In this regard, lycopene has proved to be a potent inhibitor of cell viability, arrest cell cycle and increase the apoptosis in human prostate cancer cells, suggesting an effect in the balance of human prostate cancer cell lines growth.
Nathalia Da Costa Pereira Soares; Monique De Barros Elias; Clara Lima Machado; Bruno Boquimpani Trindade; Radovan Borojevic; Anderson Junger Teodoro. Comparative Analysis of Lycopene Content from Different Tomato-Based Food Products on the Cellular Activity of Prostate Cancer Cell Lines. Foods 2019, 8, 201 .
AMA StyleNathalia Da Costa Pereira Soares, Monique De Barros Elias, Clara Lima Machado, Bruno Boquimpani Trindade, Radovan Borojevic, Anderson Junger Teodoro. Comparative Analysis of Lycopene Content from Different Tomato-Based Food Products on the Cellular Activity of Prostate Cancer Cell Lines. Foods. 2019; 8 (6):201.
Chicago/Turabian StyleNathalia Da Costa Pereira Soares; Monique De Barros Elias; Clara Lima Machado; Bruno Boquimpani Trindade; Radovan Borojevic; Anderson Junger Teodoro. 2019. "Comparative Analysis of Lycopene Content from Different Tomato-Based Food Products on the Cellular Activity of Prostate Cancer Cell Lines." Foods 8, no. 6: 201.
Anderson Junger Teodoro. Bioactive Compounds of Food: Their Role in the Prevention and Treatment of Diseases. Oxidative Medicine and Cellular Longevity 2019, 2019, 1 -4.
AMA StyleAnderson Junger Teodoro. Bioactive Compounds of Food: Their Role in the Prevention and Treatment of Diseases. Oxidative Medicine and Cellular Longevity. 2019; 2019 ():1-4.
Chicago/Turabian StyleAnderson Junger Teodoro. 2019. "Bioactive Compounds of Food: Their Role in the Prevention and Treatment of Diseases." Oxidative Medicine and Cellular Longevity 2019, no. : 1-4.
Hepatic stellate cells are liver-specific perivascular cells, identified as the major source of collagen in liver fibrosis, following their activation and conversion to myofibroblast-like cells.
Monique De Barros Elias; Felipe Leite de Oliveira; Fatima Costa Rodrigues Guma; Renata Brum Martucci; Radovan Borojevic; Anderson Junger Teodoro. Lycopene inhibits hepatic stellate cell activation and modulates cellular lipid storage and signaling. Food & Function 2019, 10, 1974 -1984.
AMA StyleMonique De Barros Elias, Felipe Leite de Oliveira, Fatima Costa Rodrigues Guma, Renata Brum Martucci, Radovan Borojevic, Anderson Junger Teodoro. Lycopene inhibits hepatic stellate cell activation and modulates cellular lipid storage and signaling. Food & Function. 2019; 10 (4):1974-1984.
Chicago/Turabian StyleMonique De Barros Elias; Felipe Leite de Oliveira; Fatima Costa Rodrigues Guma; Renata Brum Martucci; Radovan Borojevic; Anderson Junger Teodoro. 2019. "Lycopene inhibits hepatic stellate cell activation and modulates cellular lipid storage and signaling." Food & Function 10, no. 4: 1974-1984.
The objectives of this study were to assess the effect of hypoenergetic diet associated with eggplant (Solanummelongena L.) flour (EF) consumption on the antioxidant status in overweight women and compare the effect among metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO) women. This is a 4‐month double‐blind, randomised and placebo‐controlled clinical trial. The volunteers were divided into two groups: (a) balanced and individualised hypoenergetic diet + 13 g of EF (Eggplant Flour Group [EFG]); and (b) balanced and individualised hypoenergetic diet + 13 g of placebo (Placebo Group [PG]). One hundred and eighty‐six women completed the study. The EFG exhibited significant increase in antioxidant capacity after the intervention (P = 0.044). MHO exhibited statistically significant increased antioxidant capacity (P = 0.028). Body fat had significant reduction in the EFG (P = 0.010). Waist circumference was reduced in the two groups. The hypoenergetic diet associated with EF improved the antioxidant status in comparison to the PG and in MUO women the eggplant flour reduced fat mass.
Mauara Scorsatto; Glorimar Rosa; Ronir Raggio Luiz; Alessandra Da Rocha Pinheiro Mulder; Anderson Junger Teodoro; Gláucia M. Moraes De Oliveira. Effect of Eggplant Flour ( Solanum melongena L.) associated with hypoenergetic diet on antioxidant status in overweight women ‐ a randomised clinical trial. International Journal of Food Science & Technology 2019, 54, 2182 -2189.
AMA StyleMauara Scorsatto, Glorimar Rosa, Ronir Raggio Luiz, Alessandra Da Rocha Pinheiro Mulder, Anderson Junger Teodoro, Gláucia M. Moraes De Oliveira. Effect of Eggplant Flour ( Solanum melongena L.) associated with hypoenergetic diet on antioxidant status in overweight women ‐ a randomised clinical trial. International Journal of Food Science & Technology. 2019; 54 (6):2182-2189.
Chicago/Turabian StyleMauara Scorsatto; Glorimar Rosa; Ronir Raggio Luiz; Alessandra Da Rocha Pinheiro Mulder; Anderson Junger Teodoro; Gláucia M. Moraes De Oliveira. 2019. "Effect of Eggplant Flour ( Solanum melongena L.) associated with hypoenergetic diet on antioxidant status in overweight women ‐ a randomised clinical trial." International Journal of Food Science & Technology 54, no. 6: 2182-2189.