Production–living–ecological land (PLEL) is one of the research focuses of land planning and regional sustainable development in China. This paper builds a three-level classification system of PLEL based on the identification of the main land use functions (LUFs). Taking 215 typical towns in the hilly area of Sichuan Province, Southwest China as samples, the quantitative, spatial, and functional characteristics and impact factors of PLEL were studied. The results showed that (1) production land holds a dominant role in the hilly area of Sichuan Province, and production land (PL), living land (LL) and ecological land (EL) account for 66.06%, 7.60%, and 26.34% of the area, respectively. The area of agricultural production land is the largest; forestland and rural living land rank second and third. (2) The spatial patterns of PLEL in different regions of hilly area have differences. The proportion of PL gradually decreases from north to south, while the proportion of EL gradually increases from north to south, and the difference in LL is not obvious. The EL is mainly distributed in the upper and middle parts of hills, and the PL and LL are mainly distributed in the foot slopes and valleys. (3) The main functions of PLEL in the hilly area of Sichuan are production and ecology. The production function is mainly for agricultural and forestry products, and the living function is mainly for cultural leisure and residential functions. There are little differences among the ecological sub-functions. (4) There is a strong correlation between PLEL and natural–social–economic factors in the hilly area of Sichuan. Natural conditions such as latitude, relative height, and surface roughness have significant impacts on PL and EL. Social and economic factors such as population density, location and total industrial output value have a significant impact on LL. The results of this study provide valuable implications for the spatial planning and sustainable development in the Sichuan Basin and upstream of the Yangtze River.

Soil microbial characteristics are considered to be an index for soil quality evaluation. It is generally believed that organic amendments replacing chemical fertilizers have positive effects on changing microbial activity and community structure. However, their effects on different agro-ecosystems on a global scale and their differences in different environmental conditions and experimental durations are unclear. This study performed a meta-analysis based on 94 studies with 204 observations to evaluate the overall effects and their differences in different experimental conditions and duration. The results indicated that compared to chemical fertilizer, organic amendments significantly increased total microbial biomass, bacterial biomass, fungal biomass, Gram-positive bacterial biomass and Gram-negative bacterial biomass, and had no effect on the ratio of fungi to bacteria and ratio of Gram-positive bacteria to Gram-negative bacteria. Meanwhile, land use type, mean annual precipitation and soil initial pH are essential factors affecting microbial activity response. Organic-amendment-induced shifts in microbial biomass can be predominantly explained by soil C and nutrient availability changes. Additionally, we observed positive relationships between microbial functionality and microbial biomass, suggesting that organic-amendment-induced changes in microbial activities improved soil microbial functionality.

Most General Circulation Models predict more infrequent but larger precipitation events in the eastern United States combined with a warmer climate. This could have a negative effect on maize production. To understand the sensitivity of maize production to future changes in precipitation, we analyzed growing season precipitation and average state maize yields in the eastern United States for the period 1963–2011. Growing season precipitation did not show a strong trend during this period. However, crop yields increased at 3.90% in the southern, 2.62% in the central, and 2.31% in the northern part of the eastern United States, which we attributed to technology and management. To separate technology and management effects from precipitation variability, we corrected maize yields for these yield trends. We then correlated maize yield corrected for advances in technology and management with total growing season precipitation and precipitation in the critical month of pollination, from the regional to state scale. Maize yield - precipitation relationships showed that moisture shortage rather than excess determined maize yield in the Eastern United States. The relationship between total growing season precipitation-maize yield was stronger in the southeastern than in the northeastern U.S., but the critical month precipitation-maize yield relationship was stronger in the northeastern than in the southeastern U.S. If climate model predictions are accurate and total growing season precipitation will not decrease in this region but variability will, it is likely that maize yields in the northeastern U.S. will be more significantly affected.

Application of organic fertilizers or their combination with chemical fertilizers is a feasible practice for improving soil fertility and reducing soil degradation in agroecosystems, and these regulations are mainly mediated though soil microbial communities. Despite bacteria ranking among the most abundant and diverse groups of soil microorganisms, the effects of long-term organic fertilization (OF) and chemical–organic fertilization (COF) on soil bacterial diversity and community composition remain unclear. In this study, we conducted a meta-analysis and demonstrated that OF had no significant effect on bacterial alpha diversity. Application of chemical fertilizer and crop residue significantly decreased bacterial Richness index. Both OF and COF significantly altered bacterial community structure, with these changes being predominately attributed to shifts in soil pH. For bacterial phyla, both OF and COF significantly increased the relative abundance of Proteobacteria and Bacteroidetes, suggesting that OF and COF may cause the enrichment of copiotrophic taxa. In addition, COF significantly increased the relative abundance of Gammaproteobacteria but decreased the relative abundance of Acidobacteria. Overall, our results suggest that organic and chemical–organic fertilization can effectively maintain bacterial diversity and enhance soil fertility in agroecosystems, and the alteration of soil bacterial community structure is closely intertwined with soil pH.

Waste mushroom residues are often returned to fields as organic amendments. Here, we estimated the effects of the continuous applications of different spent mushroom substrates for 2 years on crop yields, soil nutrients, and heavy metals in paddy fields. The study comprised seven treatments: no fertilization (CK) and mineral NPK fertilizer (CF), as well as NPK fertilizer combined with Enoki mushroom residue (EMR50), Oyster mushroom residue (OMR50), Auricularia polytricha mushroom residue (APR50), Shiitake mushroom residue (SMR50), and Agaricus bisporus residue (ABR50). The grain yield was highest under the APR50 treatment. The short-term application of waste mushroom residue significantly increased SOC, TN, TP, and TK content relative to the CK treatment. The SOC, TP, and TK were highest under ABR50. Both total Cr and Cd contents were highest under CF treatment. The highest cumulative ecological risk was observed under OMR50 treatment. In addition, crop yield was positively correlated with SOC, TN, TP, and TP. Our results highlight that further research and innovation are needed to optimize the benefits and overcome the challenges of mushroom residue application.

The purpose of this study was to establish a spatial multi-scale integrated assessment framework for critical areas of ecosystem service supply and demand, in order to provide theoretical support for regional ecological protection planning and refined management. Taking the typical hilly area of the upper reaches of the Yangtze River in China as an example, based on the assessment matrix of land use and ecosystem services, we used the method of spatial heterogeneity assessment and self-organizing feature mapping (SOFM) to explore the identification and regionalization of critical areas of ecosystem services at regional and small scales. The results show that there was spatial heterogeneity and scale effects of ecosystem services under the two scales. The small-scale supply–demand pattern was greatly affected by microtopography and land use patterns, and the importance of ecosystem services was as follows: forest area in the upper part of the mountain > orchard and dry area in the middle and lower part of the mountain > valley farming area > flat town and farming area. The regional-scale supply–demand pattern was greatly affected by landscape structure, location conditions and social economy, and the importance of ecosystem services was as follows: south > west > north > central. The SOFM network quantitatively identified four types of ecological areas with clear dominant functions at regional and small scales. The balance between supply and demand in the studied ecosystem service areas was I < II < III < IV, in which IV was the critical area in terms of supply and I was the critical area in terms of demand. This assessment framework can improve the spatial accuracy and objectivity of the quantification and mapping of ecosystem services, and provide new ideas for multi-scale identification and expression of ecosystem services.