Background: Alterations in the fecal bacterial flora occur in inflammatory bowel disease (IBD). We examined the abundance and diversity of Clostridium leptum group, an important group of carbohydrate-fermenting bacteria, in the feces of patients with IBD and compared them with healthy controls. Methods: Seventeen healthy controls (HC), 20 patients with Crohn’s disease (CD) and 22 patients with ulcerative colitis (UC) participated in the study. DNA extracted from fecal samples was amplified by PCR targeting 16S rRNA gene sequences specific to C. leptum group. The PCR product was subjected to temporal temperature gradient electrophoresis (TTGE) and the number and position of individual bands were noted and diversity was estimated. The identity of bands at different positions was confirmed by cloning and sequencing. Real time quantitative PCR with Mesa Green, targeted at specific 16S rRNA gene sequences, was used to quantitate C. leptum group and its most prominent constituent, Faecalibacterium prausnitzii. Results: Twenty five different operational taxonomic units (OTUs, equivalent to species) were identified constituting the C. leptum group in these participants. Their sequences were deposited in GenBank [accession numbers GQ465348 to GQ465370]. OTU number was significantly reduced in CD (7.7±3.7, mean±SD) and UC (9.0±3.0) compared to HC (11.9±2.2) (P=0.0005). The Simpson D index of alpha diversity was not significantly different between the three groups. Total numbers of C. leptum group bacteria and F. prausnitzii were reduced in both CD and UC compared to HC (P=0.0036 and P<0.0001 respectively). Disease activity did not influence numbers of C. leptum or F. prausnitzii in patients with CD or UC. Conclusion: C. leptum numbers and diversity were significantly reduced in both CD and UC suggesting that alterations noted were not specific to one disease. This could contribute to reduced short chain fatty acid production in IBD.

An alternate host for mycobacteria is Mycobacterium smegmatis which is used frequently. It is a directly budding eco-friendly organism not emulated as human infection. It is mainly useful for the investigation of various microorganisms in the sort of Mycobacteria in cell culture laboratories. Some Mycobacterium species groups that is normal, unsafe ailments, likely to Mycobacterium leprae, Mycobacterium tuberculosis and Mycobacterium bovis. At present, various laboratories are clean and culture this type of species to make an opinion that fascinating route of harmful Mycobacteria. This publication provides aggregate data on cell shape, genome studies, ecology, pathology and utilization of M. smegmatis.

Activated calcium oxide extracted from crab shell impregnated on Na-ZSM-5 has been investigated. Crab shells were collected, powdered and calcined at 900 °C, and CaO was impregnated on Na-ZSM-5 and calcined at 550 °C for 10 h. The CaO/Na-ZSM-5 was characterized by X-ray diffraction, scanning electron microscopy and BET surface area. The prepared catalyst was tested for its catalytic activity by transesterifing neem oil into biodiesel in the presence of methanol. The influence of various parameters including reaction time, temperature, methanol to oil ratio, catalyst concentration and dosage were also investigated. Produced biodiesel have also been tested using proton NMR spectroscopy. Biodiesel yield as 95% has been achieved with 15% CaO impregnated on Na-ZSM-5 at 75 °C. The optimum transesterification reaction conditions were identified as follows: reaction temperature, 75 °C; reaction time, 6 h; methanol-to-neem oil molar ratio, 12:1; catalyst dosage, 0.2 g; and catalyst concentration, 15%. Based on the above study, it can be concluded that the calcium oxide impregnated Na-ZSM-5 can be a potential catalyst for biodiesel production

Photocatalysis plays a prominent role in the protection of the environment from recalcitrant pollutants by reducing hazardous wastes. Among the different methods of choice, photocatalysis mediated through nanomaterials is the most widely used and economical method for removing pollutants from wastewater. Recently, worldwide researchers focused their research on eco-friendly and sustainable environmental aspects. Wastewater contamination is one of the major threats coming from industrial processes, compared to other environmental issues. Much research is concerned with the advanced development of technology for treating wastewater discharged from various industries. Water treatment using photocatalysis is prominent because of its degradation capacity to convert pollutants into non-toxic biodegradable products. Photocatalysts are cheap, and are now emerging slowly in the research field. This review paper elaborates in detail on the metal oxides used as a nano photocatalysts in the various type of pollutant degradation. The progress of research into metal oxide nanoparticles, and their application as photocatalysts in organic pollutant degradation, were highlighted. As a final consideration, the challenges and future perspectives of photocatalysts were analyzed. The application of nano-based materials can be a new horizon in the use of photocatalysts in the near future for organic pollutant degradation.