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Phosphorus availability and acid pH are major limiting factors for grass and crop production in the Andisols of Southern Chile. Sustainable management requires both the efficient use of fertilizers and the recycling of agricultural waste. The aim of this study was to investigate the effect of citrus waste, alone or in combination with beef cattle manure (CM), on phosphorus availability. Controlled laboratory incubations were carried out for up to six weeks. Lemon (L) and mandarin (M) fruit wastes were added to an Andisol, amended or not with dried CM. Fruit parts (Peel, Juice or whole fruit), and for whole fruit the size of the fruit pieces added to soil, were compared. Water-extractable (WE) P, phosphatase activity (P-ase), and pH were monitored. In general, the size and distribution of the citrus fruit wastes gave similar trends in WE-P, P-ase, and pH. Lemon induced larger effects on WE-P and pH than did mandarin. CM caused sustained soil alkalization, increased soil WE-P and P-ase. Citrus addition produced a short-lived acidification followed by net alkalization and immediate increases in WE-P and P-ase. The combined amendment with citrus wastes enhanced the positive effect of CM on soil WE-P and alkalization, with only a small effect on P-ase. These findings indicate that citrus wastes would intensify the beneficial effects of CM amendment on soil P availability and additionally the liming effect on acid soils.
Cecilia Paredes; Siobhan Staunton; Giuseppe Di Rauso Simeone; Maria A. Rao; Antonio Violante; María De La Luz Mora. Citrus Residue Enhances the Effectiveness of Beef Cattle Manure Improving the Phosphorus Availability in Acidic Andisol. Communications in Soil Science and Plant Analysis 2021, 1 -15.
AMA StyleCecilia Paredes, Siobhan Staunton, Giuseppe Di Rauso Simeone, Maria A. Rao, Antonio Violante, María De La Luz Mora. Citrus Residue Enhances the Effectiveness of Beef Cattle Manure Improving the Phosphorus Availability in Acidic Andisol. Communications in Soil Science and Plant Analysis. 2021; ():1-15.
Chicago/Turabian StyleCecilia Paredes; Siobhan Staunton; Giuseppe Di Rauso Simeone; Maria A. Rao; Antonio Violante; María De La Luz Mora. 2021. "Citrus Residue Enhances the Effectiveness of Beef Cattle Manure Improving the Phosphorus Availability in Acidic Andisol." Communications in Soil Science and Plant Analysis , no. : 1-15.
Expansion of farmland with food production as a major service has been largely associated with conversion of natural ecosystems like the Amazon and Savanna into new agricultural land
María Mora; Marcela Calabi-Floody; Cornelia Rumpel. Closing Biogeochemical Cycles and Meeting Plant Requirements by Smart Fertilizers and Innovative Organic Amendments. Agronomy 2021, 11, 1158 .
AMA StyleMaría Mora, Marcela Calabi-Floody, Cornelia Rumpel. Closing Biogeochemical Cycles and Meeting Plant Requirements by Smart Fertilizers and Innovative Organic Amendments. Agronomy. 2021; 11 (6):1158.
Chicago/Turabian StyleMaría Mora; Marcela Calabi-Floody; Cornelia Rumpel. 2021. "Closing Biogeochemical Cycles and Meeting Plant Requirements by Smart Fertilizers and Innovative Organic Amendments." Agronomy 11, no. 6: 1158.
Soil metal contamination has emerged as a global environmental issue due to toxicity to living organisms. Traditional remediation techniques applied to metal-contaminated soils are intrusive and expensive, by which more environmental-friendly methods, such as phytoremediation, are necessary to remediate metal-contaminated soils. This study aimed to evaluate the combined effect of poultry manure compost (PMC) and indigenous arbuscular mycorrhizal (IM) fungi inoculation over copper (Cu) immobilization and the effectiveness of these elements promoting the plant growth and the soil microbial communities in a Cu-contaminated soil. A sandy Cu-contaminated soil (338 mg kg−1 of extractable Cu) was amended with PMC (2 and 4% w/w) in a pot experiment using the metallophyte Oenothera picensis. The plants were inoculated with the IM fungi isolated from the Cu-contaminated soils and grown for 3 months. PMC produced a liming effect, reduced the exchangeable Cu, and increased the amount of Cu-bound to organic and residual fractions. In addition, PMC also increased the biomass of O. picensis, which grew 8.5 times than the ones in unamended soil. Moreover, PMC incorporation stimulated soil microorganisms, increasing basal respiration and dehydrogenase activity modifying also the microbial community composition probably by reducing Cu availability and increasing carbon and nutrient sources to microorganism. On the contrary, the IM fungi inoculation reduced the plant biomass and increased the Cu translocation to the aboveground tissues. Therefore, the PMC is an effective amendment for remediating Cu-contaminated soils since it buffered soil pH, decreased Cu availability, supported the growth of O. picensis, and promoted mainly microbial activities in special shifting bacterial communities.
Sebastián Meier; Francisca Moore; Nasser Khan; María-Eugenia González; Jorge Medina; Jonathan Cumming; Arturo Morales; Paola Durán; Alex Seguel; Humberto Aponte. Effect of Poultry Manure Compost and Arbuscular Mycorrhizal Fungi on Cu Immobilization and Soil Microbial Communities in a Cu-Contaminated Soil Using the Metallophyte Oenothera Picensis. Journal of Soil Science and Plant Nutrition 2021, 1 -11.
AMA StyleSebastián Meier, Francisca Moore, Nasser Khan, María-Eugenia González, Jorge Medina, Jonathan Cumming, Arturo Morales, Paola Durán, Alex Seguel, Humberto Aponte. Effect of Poultry Manure Compost and Arbuscular Mycorrhizal Fungi on Cu Immobilization and Soil Microbial Communities in a Cu-Contaminated Soil Using the Metallophyte Oenothera Picensis. Journal of Soil Science and Plant Nutrition. 2021; ():1-11.
Chicago/Turabian StyleSebastián Meier; Francisca Moore; Nasser Khan; María-Eugenia González; Jorge Medina; Jonathan Cumming; Arturo Morales; Paola Durán; Alex Seguel; Humberto Aponte. 2021. "Effect of Poultry Manure Compost and Arbuscular Mycorrhizal Fungi on Cu Immobilization and Soil Microbial Communities in a Cu-Contaminated Soil Using the Metallophyte Oenothera Picensis." Journal of Soil Science and Plant Nutrition , no. : 1-11.
This study was conducted to evaluate the effect of compost produced with agricultural residues and oat-based biochar, iron oxide and halloysite nanoparticles as additives of the process of composting on soil chemical properties, nutrient status and growth of ryegrass Lolium perenne L. For this, a 90-day mesocosm experiment was carried out under greenhouse conditions. Bare soil and a basal fertilization treatment were compared to soils amended with nonadditive compost (NA compost), compost supplied with oat-based biochar (Bioch compost), iron oxide nanoparticles (Fe compost), and halloysite nanoparticles (Ha compost). Compost supplied with nanoparticles and biochar combined were also considered. The incorporation of compost with or without additives increased the content of total C and N in soil, with N diminishing (total and mineral forms) and C/N modifications after 90 days. The addition of compost and co-composted treatments also increased the total contents of main nutrients such as Ca, K, P and S. Furthermore, the supply of additives into composting did not increase the concentration of trace toxic elements. At the end of the experiment, plant biomass increased by the addition of the different organic amendments, with the highest shoot biomass in soils amended with compost supplied with nanoparticles. These results suggest that the addition of compost based on agricultural residues with additives such as halloysite or biochar improves chemical properties and nutritional status of soil that favor and increase plant growth of Lollium perenne stablished in soils from the Mediterranean Region.
Jorge Medina; Marcela Calabi-Floody; Humberto Aponte; Christian Santander; Marina Paneque; Sebastian Meier; Marco Panettieri; Pablo Cornejo; Fernando Borie; Heike Knicker. Utilization of Inorganic Nanoparticles and Biochar as Additives of Agricultural Waste Composting: Effects of End-Products on Plant Growth, C and Nutrient Stock in Soils from a Mediterranean Region. Agronomy 2021, 11, 767 .
AMA StyleJorge Medina, Marcela Calabi-Floody, Humberto Aponte, Christian Santander, Marina Paneque, Sebastian Meier, Marco Panettieri, Pablo Cornejo, Fernando Borie, Heike Knicker. Utilization of Inorganic Nanoparticles and Biochar as Additives of Agricultural Waste Composting: Effects of End-Products on Plant Growth, C and Nutrient Stock in Soils from a Mediterranean Region. Agronomy. 2021; 11 (4):767.
Chicago/Turabian StyleJorge Medina; Marcela Calabi-Floody; Humberto Aponte; Christian Santander; Marina Paneque; Sebastian Meier; Marco Panettieri; Pablo Cornejo; Fernando Borie; Heike Knicker. 2021. "Utilization of Inorganic Nanoparticles and Biochar as Additives of Agricultural Waste Composting: Effects of End-Products on Plant Growth, C and Nutrient Stock in Soils from a Mediterranean Region." Agronomy 11, no. 4: 767.
Engineered nanoparticles (ENPs) present in consumer products are being released into the agricultural systems. There is little information about the direct effect of ENPs on phosphorus (P) availability, which is an essential nutrient for crop growth naturally occurring in agricultural soils. The present study examined the effect of 1, 3, and 5% doses of Cu0 or Ag0 ENPs stabilized with L-ascorbic acid (suspension pH 2–3) on P ad- and desorption in an agricultural Andisol with total organic matter (T-OM) and with partial removal of organic matter (R-OM) by performing batch experiments. Our results showed that the adsorption kinetics data of H2PO4 − on T-OM and R-OM soil samples with and without ENPs were adequately described by the pseudo-second-order (PSO) and Elovich models. The adsorption isotherm data of H2PO4 − from T-OM and R-OM soil samples following ENPs addition were better fitted by the Langmuir model than the Freundlich model. When the Cu0 or Ag0 ENPs doses were increased, the pH value decreased and H2PO4 − adsorption increased on T-OM and R-OM. The H2PO4 − desorption (%) was lower with Cu0 ENPs than Ag0 ENPs. Overall, the incorporation of ENPs into Andisols generated an increase in P retention, which may affect agricultural crop production.
Jonathan Suazo-Hernández; Erwin Klumpp; Nicolás Arancibia-Miranda; Patricia Poblete-Grant; Alejandra Jara; Roland Bol; María De La Luz Mora. Describing Phosphorus Sorption Processes on Volcanic Soil in the Presence of Copper or Silver Engineered Nanoparticles. Minerals 2021, 11, 373 .
AMA StyleJonathan Suazo-Hernández, Erwin Klumpp, Nicolás Arancibia-Miranda, Patricia Poblete-Grant, Alejandra Jara, Roland Bol, María De La Luz Mora. Describing Phosphorus Sorption Processes on Volcanic Soil in the Presence of Copper or Silver Engineered Nanoparticles. Minerals. 2021; 11 (4):373.
Chicago/Turabian StyleJonathan Suazo-Hernández; Erwin Klumpp; Nicolás Arancibia-Miranda; Patricia Poblete-Grant; Alejandra Jara; Roland Bol; María De La Luz Mora. 2021. "Describing Phosphorus Sorption Processes on Volcanic Soil in the Presence of Copper or Silver Engineered Nanoparticles." Minerals 11, no. 4: 373.
The monitoring of soil metal(loid) contamination is of global significance due to deleterious effects that metal(loid)s have on living organisms. Soil biological properties such as enzyme activities (EAs) are good indicators of metal(loid) contamination due to their high sensitivity, fast response, and low-cost. Here, the effect of metal(loid) contamination on physicochemical properties and microbial functionality in soils sampled from within 10 km of a Cu smelter is investigated. Soil composite samples were randomly taken within 2, 4, 6, 8 and10 km zones from a mining industry Cu smelter. The EAs of dehydrogenase (DHA), arylsulfatase (ARY), β-glucosidase, urease, and arginine ammonification (AA) were studied as indicators of metal(loid) contamination, which included the ecological dose (ED50) with respect to Cu and As contents. The community level physiological profile (CLPP), functional diversity, and catabolic evenness were evaluated based on the C-substrate utilisation. All EAs decreased in zones with high degrees of metal(loid) contamination, which also had low TOC and clay contents, reflecting long term processes of soil degradation. Positive and strong relationships between EAs and TOC were found. DHA and ARY activities decreased by approximately 85–90% in highly metal(loid) contaminated soils. DHA and AA showed significant ED50 values associated with available Cu (112.8 and 121.6 mg CuDTPA kg−1, respectively) and total As contents (30.8 and 31.8 mg As kg−1, respectively). The CLPP showed different metabolic profiles along the metal(loid) contamination gradients. Long-term stress conditions in soils close to industrial areas resulted in the decreasing of general biological activity, catabolic capacity, and functional diversity.
Humberto Aponte; Pedro Mondaca; Christian Santander; Sebastián Meier; Jorge Paolini; Benjamin Butler; Claudia Rojas; María Cristina Diez; Pablo Cornejo. Enzyme activities and microbial functional diversity in metal(loid) contaminated soils near to a copper smelter. Science of The Total Environment 2021, 779, 146423 .
AMA StyleHumberto Aponte, Pedro Mondaca, Christian Santander, Sebastián Meier, Jorge Paolini, Benjamin Butler, Claudia Rojas, María Cristina Diez, Pablo Cornejo. Enzyme activities and microbial functional diversity in metal(loid) contaminated soils near to a copper smelter. Science of The Total Environment. 2021; 779 ():146423.
Chicago/Turabian StyleHumberto Aponte; Pedro Mondaca; Christian Santander; Sebastián Meier; Jorge Paolini; Benjamin Butler; Claudia Rojas; María Cristina Diez; Pablo Cornejo. 2021. "Enzyme activities and microbial functional diversity in metal(loid) contaminated soils near to a copper smelter." Science of The Total Environment 779, no. : 146423.
The current scientific evidence shows that phosphorus (P) appears to mitigate the adverse effects derived from water deficit stress. However, the efficiency of wheat in the use of both components have been not addressed. The objective of this study was to investigate the combined effect of P and water limiting conditions over phosphorus utilization efficiency (PUTE) and water use efficiency (WUE) to categorize different wheat genotypes. Ten wheat genotypes, including old, current and advanced lines were screened for their ability to utilize phosphorus (P) and water. Plants were grown under controlled conditions on an Andisol with soil P-concentration of 4 mg P kg−1 (Inadequate P-4 P-), which was enriched to 30 mg P kg−1 (Adequate P-30 P-). Irrigation was applied at two levels: adequate irrigation (W1) and 30% of W1 (Inadequate irrigation -W2-). Wheat was grown until end of its phenological cycle. The inadequate P and water produced a long delay on plant development, especially on early vegetative stages. As expected, the genotypes showed differences in their growth, development, P accumulation and yield in response to inadequate P and water conditions. The lowest performance in terms of plant growth, development and yield was observed when both the P and water restrictions were applied. Genotypic differences were observed in terms of utilization of P and water. On this basis, wheat genotypes were grouped into three and four classes for PUTE WUE, respectively. The most efficient genotype for all the conditions was “Fritz”, which obtained the highest score in the consolidated ranking for PUTE and WUE. On the contrary, “Kirón” was the most inefficient genotype. A strong correlation (~85%) was observed between PUTE and WUE, which could enable the future selection of co-adapted wheat genotypes efficient in the utilization of P and water.
Sebastián Meier; Francisca Moore; Arturo Morales; Claudio Jobet; Rafael López-Olivari; Humberto Aponte; Paula Cartes; Pedro Campos; Naser Khan. Interactive role between phosphorus utilization efficiency and water use efficiency. A tool to categorize wheats co-adapted to water and phosphorus limiting conditions. Agricultural Water Management 2021, 248, 106765 .
AMA StyleSebastián Meier, Francisca Moore, Arturo Morales, Claudio Jobet, Rafael López-Olivari, Humberto Aponte, Paula Cartes, Pedro Campos, Naser Khan. Interactive role between phosphorus utilization efficiency and water use efficiency. A tool to categorize wheats co-adapted to water and phosphorus limiting conditions. Agricultural Water Management. 2021; 248 ():106765.
Chicago/Turabian StyleSebastián Meier; Francisca Moore; Arturo Morales; Claudio Jobet; Rafael López-Olivari; Humberto Aponte; Paula Cartes; Pedro Campos; Naser Khan. 2021. "Interactive role between phosphorus utilization efficiency and water use efficiency. A tool to categorize wheats co-adapted to water and phosphorus limiting conditions." Agricultural Water Management 248, no. : 106765.
Different techniques have been developed for the remediation of Cu contaminated soils, being the phytoremediation a sustainable and environmentally friendly strategy, but its use in mine tailings is scarce. Arbuscular mycorrhizal fungi (AMF) can decrease the Cu concentration in plants by favouring the stabilization of this metal through different mechanisms such as the production of glomalin, immobilization in the fungal wall of hyphae and spores, and the storage of Cu in vacuoles. Additionally, the use of organic amendments promotes the beneficial effects produced by AMF and improves plant growth. Based on the above, the aim of this study was to determine the effect of AMF inoculation and compost application at different doses on the growth of Oenothera picensis in a Cu mine tailing. One group of plants were inoculated with Claroideoglomus claroideum (CC) and other was non-inoculated (NM). Both CC and NM were grown for two month under greenhouse conditions in pots with the Cu mine tailing, which also had increasing compost doses (0%, 2.5%, 5%, and 10%). Results showed greater biomass production of O. picensis by CC up to 2-fold compared with NM. This effect was improved by the compost addition, especially at doses of 5% and 10%. Therefore, the increase of mycorrhizal and nutritional parameters in O. picensis, and the decreasing of Cu availability in the mine tailing, promoted the production of photosynthetic pigments together with the plant growth, which is of importance to accomplish phytoremediation programs in Cu mine tailings.
Rodrigo Pérez; Yasna Tapia; Monica Antilén; Manuel Casanova; Catalina Vidal; Christian Santander; Humberto Aponte; Pablo Cornejo. Interactive effect of compost application and inoculation with the fungus Claroideoglomus claroideum in Oenothera picensis plants growing in mine tailings. Ecotoxicology and Environmental Safety 2020, 208, 111495 .
AMA StyleRodrigo Pérez, Yasna Tapia, Monica Antilén, Manuel Casanova, Catalina Vidal, Christian Santander, Humberto Aponte, Pablo Cornejo. Interactive effect of compost application and inoculation with the fungus Claroideoglomus claroideum in Oenothera picensis plants growing in mine tailings. Ecotoxicology and Environmental Safety. 2020; 208 ():111495.
Chicago/Turabian StyleRodrigo Pérez; Yasna Tapia; Monica Antilén; Manuel Casanova; Catalina Vidal; Christian Santander; Humberto Aponte; Pablo Cornejo. 2020. "Interactive effect of compost application and inoculation with the fungus Claroideoglomus claroideum in Oenothera picensis plants growing in mine tailings." Ecotoxicology and Environmental Safety 208, no. : 111495.
Nanoscale zero-valent iron (NZVI) and NZVI supported onto montmorillonite (NZVI-Mt) were synthetized and used in this study to remove SeVI and AsV from water in mono- and binary-adsorbate systems. The adsorption kinetics and isotherm data for SeVI and AsV were adequately described by the pseudo-second-order (PSO) (r2>0.94) and Freundlich (r2>0.93) equations. Results from scanning electron microscopy showed that the dimension of the NZVI immobilized on the Mt was smaller than pure NZVI. Using 0.05 g of adsorbent and an initial 200 mg L−1 AsV and SeVI concentration, the maximum adsorption capacity (qmax) and partition coefficient (PC) for AsV on NZVI-Mt in monocomponent system were 54.75 mg g-1 and 0.065 mg g-1·µM-1, which dropped respectively to 49.91 mg g-1 and 0.055 mg g-1·µM-1 under competitive system. For SeVI adsorption on NZVI-Mt in monocomponent system, qmax and PC were 28.63 mg g-1 and 0.024 mg g-1·µM-1, respectively. Values of qmax and PC were higher for NZVI-Mt than NZVI and montmorillonite, indicating that the nanocomposite contained greater adsorption sites for removing both oxyanions, but with a marked preference for AsV. Future research should evaluate the effect of different operational variables on the removal efficiency of both oxyanions by NZVI-Mt.
Jonathan Suazo-Hernández; Karen Manquián-Cerda; María De La Luz Mora; Mauricio Molina-Roco; María Angélica Rubio; Binoy Sarkar; Nanthi Bolan; Nicolás Arancibia-Miranda. Efficient and selective removal of SeVI and AsV mixed contaminants from aqueous media by montmorillonite-nanoscale zero valent iron nanocomposite. Journal of Hazardous Materials 2020, 403, 123639 .
AMA StyleJonathan Suazo-Hernández, Karen Manquián-Cerda, María De La Luz Mora, Mauricio Molina-Roco, María Angélica Rubio, Binoy Sarkar, Nanthi Bolan, Nicolás Arancibia-Miranda. Efficient and selective removal of SeVI and AsV mixed contaminants from aqueous media by montmorillonite-nanoscale zero valent iron nanocomposite. Journal of Hazardous Materials. 2020; 403 ():123639.
Chicago/Turabian StyleJonathan Suazo-Hernández; Karen Manquián-Cerda; María De La Luz Mora; Mauricio Molina-Roco; María Angélica Rubio; Binoy Sarkar; Nanthi Bolan; Nicolás Arancibia-Miranda. 2020. "Efficient and selective removal of SeVI and AsV mixed contaminants from aqueous media by montmorillonite-nanoscale zero valent iron nanocomposite." Journal of Hazardous Materials 403, no. : 123639.
Silicon (Si) exerts beneficial effects in mitigating aluminum (Al) toxicity in different plant species. These include attenuating oxidative damage and improving structural strengthening as a result of the increased production of secondary metabolites such as phenols. The aim of this research was to evaluate the effect of Si on phenol production and composition in two barley cultivars under Al stress. Our conceptual approach included a hydroponic experiment with an Al-tolerant (Sebastian) and an Al-sensitive (Scarlett) barley cultivar treated with two Al doses (0 or 0.2 mM of Al) and two Si doses (0 or 2 mM) for 21 days. Chemical, biochemical and growth parameters were assayed after harvest. Our results indicated that the Al and Si concentration decreased in both cultivars when Al and Si were added in combination. Silicon increased the antioxidant activity and soluble phenol concentration, but reduced lipid peroxidation irrespective of the Al dose. Both barley cultivars showed changes in culm creep rate, flavonoids and flavones concentration, lignin accumulation and altered lignin composition in Si and Al treatments. We concluded that Si fertilization could increase the resistance of barley to Al toxicity by regulating the metabolism of phenolic compounds with antioxidant and structural functions.
Isis Vega; Cornelia Rumpel; Antonieta Ruíz; Maria Luz Mora; Daniel Calderini; Paula Cartes. Silicon Modulates the Production and Composition of Phenols in Barley under Aluminum Stress. Agronomy 2020, 10, 1138 .
AMA StyleIsis Vega, Cornelia Rumpel, Antonieta Ruíz, Maria Luz Mora, Daniel Calderini, Paula Cartes. Silicon Modulates the Production and Composition of Phenols in Barley under Aluminum Stress. Agronomy. 2020; 10 (8):1138.
Chicago/Turabian StyleIsis Vega; Cornelia Rumpel; Antonieta Ruíz; Maria Luz Mora; Daniel Calderini; Paula Cartes. 2020. "Silicon Modulates the Production and Composition of Phenols in Barley under Aluminum Stress." Agronomy 10, no. 8: 1138.
C. Rumpel; Vannak Ann; H. Bahri; M. Calabi Floody; S. Cheik; T.T. Doan; A. Harit; J.L. Janeau; P. Jouquet; Maria Luz Mora; P. Podwojewski; T.M. Tran; Q.A. Ngo; P.L. Rossi; M. Sanaullah. Research for development in the 21st century. Geoderma 2020, 378, 114558 -114558.
AMA StyleC. Rumpel, Vannak Ann, H. Bahri, M. Calabi Floody, S. Cheik, T.T. Doan, A. Harit, J.L. Janeau, P. Jouquet, Maria Luz Mora, P. Podwojewski, T.M. Tran, Q.A. Ngo, P.L. Rossi, M. Sanaullah. Research for development in the 21st century. Geoderma. 2020; 378 ():114558-114558.
Chicago/Turabian StyleC. Rumpel; Vannak Ann; H. Bahri; M. Calabi Floody; S. Cheik; T.T. Doan; A. Harit; J.L. Janeau; P. Jouquet; Maria Luz Mora; P. Podwojewski; T.M. Tran; Q.A. Ngo; P.L. Rossi; M. Sanaullah. 2020. "Research for development in the 21st century." Geoderma 378, no. : 114558-114558.
Soil degradation by metal and metalloid (metal[loid]) contamination represents a widespread environmental threat. Most studies investigating soil metal(loid) contamination have disregarded the soil quality concept. Enzyme activities (EAs) are good soil quality indicators due to their direct connection to functions related to C, N, P, and S cycles and their sensitivity to metal(loid) contamination. This review (a) provides an overview of the development of soil quality indices (SQIs) based on EAs in metal(loid) contaminated soils, and (b) evaluates the effects of individual metal(loid)s and their combinations on the activities of the most common enzymes involved in the C, N, P, and S cycles. Four intracellular mechanisms dominate the inhibition of EAs by metal(loid)s: (a) transcription inhibition, (b) protein denaturation, (c) cell division inhibition, (d) cell membrane disruption. These mechanisms can also be further exacerbated in soil by nonspecific exoenzyme inhibition. The main EAs used as indicators for metal(loid) contaminated soils are dehydrogenase (DHA), arylsulfatase (ARY), urease (UA), acid phosphatase (Pacid), alkaline phosphatase (Palk), and catalase (CAT). These enzymes are sensitive to metal(loid) contamination, with DHA and ARY being the most sensitive indicators (62 and 56% inhibition, respectively, when averaged over all metal[loid] contamination levels). Other enzymes are inhibited by 32% (UA), 23% (Palk), 18% (Pacid), and 18% (CAT). We suggest principles for the development of SQIs considering biotic soil functions via the use of EAs. This review is the first presenting SQIs based on EAs for metal(loid) contaminated soils, and represents the first quantitative analysis of metal(loid) effects on EAs.
Humberto Aponte; Jorge Medina; Benjamin Butler; Sebastian Meier; Pablo Cornejo; Yakov Kuzyakov. Soil quality indices for metal(loid) contamination: An enzymatic perspective. Land Degradation & Development 2020, 31, 2700 -2719.
AMA StyleHumberto Aponte, Jorge Medina, Benjamin Butler, Sebastian Meier, Pablo Cornejo, Yakov Kuzyakov. Soil quality indices for metal(loid) contamination: An enzymatic perspective. Land Degradation & Development. 2020; 31 (17):2700-2719.
Chicago/Turabian StyleHumberto Aponte; Jorge Medina; Benjamin Butler; Sebastian Meier; Pablo Cornejo; Yakov Kuzyakov. 2020. "Soil quality indices for metal(loid) contamination: An enzymatic perspective." Land Degradation & Development 31, no. 17: 2700-2719.
Climate change directly affecting the Antarctic Peninsula has been reported to induce the successful colonization of ice-free lands by two Antarctic vascular plants (Deschampsia antarctica and Colobanthus quitensis). While studies have revealed the importance of microbiota for plant growth and stress tolerance in temperate climates, the role that plant-associated microbes play in the colonization of ice-free lands remains unknown. Consequently, we used high-throughput DNA sequence analyses to explore the composition, predicted functions, and interactive networks of plant-associated microbial communities among the rhizosphere, endosphere, and phyllosphere niches of D. antarctica and C. quitensis. Here we report a greater number of operational taxonomic units (OTUs), diversity, and richness in the microbial communities from the rhizosphere, relative to endosphere and phyllosphere. While taxonomic assignments showed greater relative abundances of Proteobacteria, Bacteroidetes, and Actinobacteria in plant niches, principal coordinate analysis revealed differences among the bacterial communities from the other compartments examined. More importantly, however, our results showed that most of OTUs were exclusively found in each plant niche. Major predicted functional groups of these microbiota were attributed to heterotrophy, aerobic heterotrophy, fermentation, and nitrate reduction, independent of plant niches or plant species. Co-occurrences network analyses identified 5 (e.g., Microbacteriaceae, Pseudomonaceae, Lactobacillaceae, and Corynebacteriaceae), 23 (e.g., Chitinophagaceae and Sphingomonadaceae) and 7 (e.g., Rhodospirillaceae) putative keystone taxa present in endosphere, phyllosphere, and rhizosphere, respectively. Our results revealed niche differentiation in Antarctic vascular plants, highlighting some putative microbial indicators and keystone taxa in each niche. However, more studies are required to determine the pivotal role that these microbes play in the successful colonization of ice-free lands by Antarctic plants.
Qian Zhang; Jacquelinne J. Acuña; Nitza G. Inostroza; Paola Duran; Maria Luz Mora; Michael Sadowsky; Milko A. Jorquera. Niche Differentiation in the Composition, Predicted Function, and Co-occurrence Networks in Bacterial Communities Associated With Antarctic Vascular Plants. Frontiers in Microbiology 2020, 11, 1036 .
AMA StyleQian Zhang, Jacquelinne J. Acuña, Nitza G. Inostroza, Paola Duran, Maria Luz Mora, Michael Sadowsky, Milko A. Jorquera. Niche Differentiation in the Composition, Predicted Function, and Co-occurrence Networks in Bacterial Communities Associated With Antarctic Vascular Plants. Frontiers in Microbiology. 2020; 11 ():1036.
Chicago/Turabian StyleQian Zhang; Jacquelinne J. Acuña; Nitza G. Inostroza; Paola Duran; Maria Luz Mora; Michael Sadowsky; Milko A. Jorquera. 2020. "Niche Differentiation in the Composition, Predicted Function, and Co-occurrence Networks in Bacterial Communities Associated With Antarctic Vascular Plants." Frontiers in Microbiology 11, no. : 1036.
Enzyme activities (EAs) respond to contamination in several ways depending on the chemical form and content of heavy metals and metalloids (HMs) and their interactions with various soil properties. A systematic and mechanistic understanding of EA responses to HM contamination in soil is necessary for predicting the consequences for nutrient availability and the cycling of carbon (C), nitrogen (N), phosphorus (P) and sulphur (S). In this study, a meta-analysis based on 671 observations found the activities of seven enzymes to decrease in response to soil contamination with Pb, Zn, Cd, Cu and As. HM contamination linearly reduced the activities of all enzymes in the following order: arylsulfatase > dehydrogenase > β-glucosidase > urease > acid phosphatase > alkaline phosphatase > catalase. The activities of two endoenzymes: arylsulfatase (partly as exoenzyme) and dehydrogenase were reduced by 72% and 64%, respectively. These reductions were two times greater than of exoenzymes: β-glucosidase, urease, acid phosphatase, alkaline phosphatase and catalase (partly endoenzyme). This reflects the much stronger impact of HMs on living microorganisms and their endoenzymes than on extracellular enzymes stabilized on clay minerals and organic matter. Increasing clay content weakened the negative effects of HM contamination on EAs. All negative effects of HMs on EAs decreased with soil depth because HMs remain mainly in the topsoil. EAs involved in the cycling of C and S were more affected by HMs than the enzymes associated with the cycling of N and P. Consequently, HM contamination may alter the stoichiometry of C, N, P and S released by enzymatic decomposition of organic compounds that consequently affect microbial community structure and activity.
Humberto Aponte; Paula Meli; Benjamin Butler; Jorge Paolini; Francisco Matus; Carolina Merino; Pablo Cornejo; Yakov Kuzyakov. Meta-analysis of heavy metal effects on soil enzyme activities. Science of The Total Environment 2020, 737, 139744 .
AMA StyleHumberto Aponte, Paula Meli, Benjamin Butler, Jorge Paolini, Francisco Matus, Carolina Merino, Pablo Cornejo, Yakov Kuzyakov. Meta-analysis of heavy metal effects on soil enzyme activities. Science of The Total Environment. 2020; 737 ():139744.
Chicago/Turabian StyleHumberto Aponte; Paula Meli; Benjamin Butler; Jorge Paolini; Francisco Matus; Carolina Merino; Pablo Cornejo; Yakov Kuzyakov. 2020. "Meta-analysis of heavy metal effects on soil enzyme activities." Science of The Total Environment 737, no. : 139744.
The hyphae and spores of arbuscular mycorrhizal (AM) fungi represent an essential component in the extraradical zone due to their role in nutrients and water uptake and as propagules that allow the perpetuation of the AM symbiosis over time, respectively. However, the attention of scientific literature is usually more focused on root colonization than on the study of the extraradical components of AM fungi, especially their vital, active, or functional fractions. This chapter presents some easy-to-use alternatives for staining vital, active, or functional structures of AM fungi for their subsequent microscopic visualization, such as the application of enzyme-based stains, NADPH formation, and also nucleus staining. Some modified methods for the extraction of mycelium from the soil are also presented.
Pablo Cornejo; Humberto Aponte. Visualization of Arbuscular Mycorrhizal Fungal Extraradical Hyphae and Spores Vitality and Activity. Methods in Molecular Biology 2020, 2146, 61 -71.
AMA StylePablo Cornejo, Humberto Aponte. Visualization of Arbuscular Mycorrhizal Fungal Extraradical Hyphae and Spores Vitality and Activity. Methods in Molecular Biology. 2020; 2146 ():61-71.
Chicago/Turabian StylePablo Cornejo; Humberto Aponte. 2020. "Visualization of Arbuscular Mycorrhizal Fungal Extraradical Hyphae and Spores Vitality and Activity." Methods in Molecular Biology 2146, no. : 61-71.
Highbush blueberry fruits are very perishable, decreasing quality and antioxidant potential during postharvest storage. Several preservation technologies to improve blueberries shelf life have been proposed. We evaluated the impact of fruit cold-storage and UV-C irradiation postharvest treatments on quality and antioxidant properties of berries from different blueberry cultivars grown in southern Chile. Berries of Legacy, Brigitta, and Bluegold cultivars were subjected to cold-storage (4 °C, for 28 days) or UV-C irradiation (2.3 or 4.6 kJ m−2) before cold-storage (4 °C) during 5 or 10 days. Then, fresh weight (FW), total soluble solids (TSS), titratable acidity (TiA), radical scavenging activity (RSA), total phenol content (TPC), and total anthocyanin content (TAC) were analyzed in whole fruits. During cold-storage, fruit FW was reduced (~ 20%) from day 7 onward, without variations among storage times. The irradiation of fruits with UV-C light also tends to reduce berry FW (~ 20%), but only in cultivar Legacy and in response to the highest UV-C doses. No significant differences were found in TSS or TiA of fruits by the effect of any treatments. Interestingly, UV-C irradiation induced greater antioxidant properties of blueberries. Bluegold and Brigitta fruits revealed increased RSA under 4.6 kJ m−2 doses. Also, Bluegold showed the highest levels of TPC at doses of 4.6 kJ m−2 of UV-C. Moreover, fruits of all cultivars exhibited increased TCA in response to the higher doses of UV-C. UV-C irradiation could be an interesting tool to improve antioxidant potential in highbush blueberries, which can negatively affect fruit quality for fresh consumption.
J. González-Villagra; M. Reyes-Díaz; M. Alberdi; Maria Luz Mora; E. M. Ulloa-Inostroza; A. E. Ribera-Fonseca. Impact of Cold-Storage and UV-C Irradiation Postharvest Treatments on Quality and Antioxidant Properties of Fruits from Blueberry Cultivars Grown in Southern Chile. Journal of Soil Science and Plant Nutrition 2020, 20, 1751 -1758.
AMA StyleJ. González-Villagra, M. Reyes-Díaz, M. Alberdi, Maria Luz Mora, E. M. Ulloa-Inostroza, A. E. Ribera-Fonseca. Impact of Cold-Storage and UV-C Irradiation Postharvest Treatments on Quality and Antioxidant Properties of Fruits from Blueberry Cultivars Grown in Southern Chile. Journal of Soil Science and Plant Nutrition. 2020; 20 (4):1751-1758.
Chicago/Turabian StyleJ. González-Villagra; M. Reyes-Díaz; M. Alberdi; Maria Luz Mora; E. M. Ulloa-Inostroza; A. E. Ribera-Fonseca. 2020. "Impact of Cold-Storage and UV-C Irradiation Postharvest Treatments on Quality and Antioxidant Properties of Fruits from Blueberry Cultivars Grown in Southern Chile." Journal of Soil Science and Plant Nutrition 20, no. 4: 1751-1758.
This study was carried out to evaluate the effects of foliar sprays containing boron (B) nano-fertilizer (NF) on the growth and physiology of lettuce (Lactuca sativa) and zucchini (Cucurbita pepo). Plants were grown under greenhouse conditions for 60 days on a modified Hoagland solution with the presence and absence of boron (+B or –B). A synthesized B–NF foliar spray and a commercial B foliar fertilizer (Bortrac™ 150, BT) was applied at a concentration of 30 mg B L−1 at 10-d intervals throughout the experiment. The B–NF treatment increased the growth of lettuce 2.7- and 1.9-fold for shoots and roots, respectively, with an average production of lettuce biomass by ∼58%. Similarly, the NF increased the growth of zucchini by 18 and 66% compared with Control–B (the absence of B), and 13 and 36% compared with BT, both for shoots and roots, respectively. Nevertheless, NF + B mostly decreased lettuce growth with symptoms of B toxicity in leaves. In lettuce, addition of B did not affect concentrations of phenols; however, in zucchini, Control–B induced a higher production of phenolic compounds possibly related to B deficiency. The B addition in lettuce reduced the DPPH activity by 32 and 21% in NF and BT, respectively, compared to Control-B. These responses were similar in zucchini; however, the effect of B was product of its presence in mineral solution rather than due the foliar product applied. This suggests that a NF-based delivery system for B may be highly effective at boosting plant productivity on B-limited soils.
Sebastián Meier; Francisca Moore; Arturo Morales; María-Eugenia González; Alex Seguel; Cristian Meriño-Gergichevich; Olga Rubilar; Jonathan Cumming; Humberto Aponte; David Alarcón; Jaime Mejías. Synthesis of calcium borate nanoparticles and its use as a potential foliar fertilizer in lettuce (Lactuca sativa) and zucchini (Cucurbita pepo). Plant Physiology and Biochemistry 2020, 151, 673 -680.
AMA StyleSebastián Meier, Francisca Moore, Arturo Morales, María-Eugenia González, Alex Seguel, Cristian Meriño-Gergichevich, Olga Rubilar, Jonathan Cumming, Humberto Aponte, David Alarcón, Jaime Mejías. Synthesis of calcium borate nanoparticles and its use as a potential foliar fertilizer in lettuce (Lactuca sativa) and zucchini (Cucurbita pepo). Plant Physiology and Biochemistry. 2020; 151 ():673-680.
Chicago/Turabian StyleSebastián Meier; Francisca Moore; Arturo Morales; María-Eugenia González; Alex Seguel; Cristian Meriño-Gergichevich; Olga Rubilar; Jonathan Cumming; Humberto Aponte; David Alarcón; Jaime Mejías. 2020. "Synthesis of calcium borate nanoparticles and its use as a potential foliar fertilizer in lettuce (Lactuca sativa) and zucchini (Cucurbita pepo)." Plant Physiology and Biochemistry 151, no. : 673-680.
The use of high-throughput DNA sequencing (HTS) has revealed the great diversity of rhizobacteria in plant rhizospheres; however, only a minor portion (≤ 1%) of rhizobacteria belonging to few taxa can be cultured under laboratory conditions. In recent years, in situ cultivation has opened a window to explore a greater diversity of bacterial taxa in the environment. Here, we explored the total and culturable rhizobacterial communities associated with the rhizosphere of wheat plants by using 16S rRNA-based HTS and in situ cultivation with microwell chambers (MWCs), respectively. Results by HTS revealed to phyla Proteobacteria (29–39%), Acidobacteria (17%), Actinobacteria (11–15%), and Bacteroidetes (5–12%) as the most abundant rhizobacterial taxa in rhizosphere samples. A total of 206 isolates (26 genera) were obtained with MWCs, where coincidentally with HTS, the most abundant phyla were Proteobacteria (70.4%), Firmicutes (24%), Actinobacteria (4%), and Bacteroidetes (1.5%). At the genus level, the most of isolates (72%) belonged to Pseudomonas, followed by Bacillus, Stenotrophomonas, Delftia, and Herbaspirillum. Members of rare taxa (Lelliottia, Rhodococcus, Micrococcus, Variovorax, and Bosea) also were isolated by MWCs. In addition, a high proportion (82%) of isolates showed high similarity with plant beneficial and environmental non-pathogenic bacteria whereas a minor proportion (18%) of isolates showed high similarity to human and plant pathogenic bacteria. This study demonstrates that in situ cultivation represents a useful tool to isolate a greater number of rhizobacterial taxa, which can be investigated under laboratory conditions in bioprospecting (e.g., plant growth–promoting bacteria) and public health (e.g., human opportunist and plant pathogens) studies.
Jacquelinne J. Acuña; Luis Marileo; Macarena A. Araya; Joaquin I. Rilling; Giovanni A. Larama; Maria Luz Mora; Slava Epstein; Milko A. Jorquera. In Situ Cultivation Approach to Increase the Culturable Bacterial Diversity in the Rhizobiome of Plants. Journal of Soil Science and Plant Nutrition 2020, 20, 1411 -1426.
AMA StyleJacquelinne J. Acuña, Luis Marileo, Macarena A. Araya, Joaquin I. Rilling, Giovanni A. Larama, Maria Luz Mora, Slava Epstein, Milko A. Jorquera. In Situ Cultivation Approach to Increase the Culturable Bacterial Diversity in the Rhizobiome of Plants. Journal of Soil Science and Plant Nutrition. 2020; 20 (3):1411-1426.
Chicago/Turabian StyleJacquelinne J. Acuña; Luis Marileo; Macarena A. Araya; Joaquin I. Rilling; Giovanni A. Larama; Maria Luz Mora; Slava Epstein; Milko A. Jorquera. 2020. "In Situ Cultivation Approach to Increase the Culturable Bacterial Diversity in the Rhizobiome of Plants." Journal of Soil Science and Plant Nutrition 20, no. 3: 1411-1426.
Andisols, rich in minerals like allophane, imogolite and iron- (Fe) or aluminum- (Al) oxides have high phosphorus (P) sorption capacity and require annual P additions to ensure plant productivity. It is known that the use of composted poultry manure (PM) increases soil labile P and carbon concentration, although the mechanisms controlling P availability and soil organic carbon (SOC) sequestration are not fully understood. The aim of this study was to investigate the relationship between soil available P and particles size distribution, SOC, and iron and aluminum complexes on Southern Chilean pastures with long-term PM amendment. Soil samples, were collected from 4 pastoral farms where PM had been applied annually at 3 tons ha−1 for 5 and 10 years. We analyzed for elemental concentrations, P forms through a modified P fractionation methodology and aggregation through particle size analyses and scanning electron microscopy. Moreover, we determined oxalate and pyrophosphate extractable Fe and Al. Andisols receiving long-term PM amendment had 22–65% higher SOC contents than unfertilized control likely due to improved aggregation capacity as indicated by greater soil particle size. Moreover, the readily available inorganic P increased by 56 to 286% between control and PM amended soils. Andisols amended with PM also showed lower oxalate extractable Al and Fe and higher pyrophosphate extractable forms. These parameters correlated with SOC, labile P concentrations and particle size distribution. We concluded that increased P availability under pastures growing on Andisol with long-term PM amendment is regulated by three mechanisms related to input of readily available P, release of native soil P and decreased availability of sorption sites.
Patricia Poblete-Grant; Jonathan Marcelo Suazo Hernandez; Leo Condron; Cornelia Rumpel; Rolando Demanet; Sparkle L. Malone; Maria Luz Mora. Soil available P, soil organic carbon and aggregation as affected by long-term poultry manure application to Andisols under pastures in Southern Chile. Geoderma Regional 2020, 21, e00271 .
AMA StylePatricia Poblete-Grant, Jonathan Marcelo Suazo Hernandez, Leo Condron, Cornelia Rumpel, Rolando Demanet, Sparkle L. Malone, Maria Luz Mora. Soil available P, soil organic carbon and aggregation as affected by long-term poultry manure application to Andisols under pastures in Southern Chile. Geoderma Regional. 2020; 21 ():e00271.
Chicago/Turabian StylePatricia Poblete-Grant; Jonathan Marcelo Suazo Hernandez; Leo Condron; Cornelia Rumpel; Rolando Demanet; Sparkle L. Malone; Maria Luz Mora. 2020. "Soil available P, soil organic carbon and aggregation as affected by long-term poultry manure application to Andisols under pastures in Southern Chile." Geoderma Regional 21, no. : e00271.
A critical aspect of predicting soil organic carbon (SOC) concentrations is the lack of available soil information; where information on soil characteristics is available, it is usually focused on regions of high agricultural interest. To date, in Chile, a large proportion of the SOC data have been collected in areas of intensive agricultural or forestry use; however, vast areas beyond these forms of land use have few or no soil data available. Here we present a new SOC database for the country, which is the result of an unprecedented national effort under the framework of the Global Soil Partnership. This partnership has helped build the largest database of SOC to date in Chile, named the Chilean Soil Organic Carbon database (CHLSOC), comprising 13 612 data points compiled from numerous sources, including unpublished and difficult-to-access data. The database will allow users to fill spatial gaps where no SOC estimates were publicly available previously. Presented values of SOC range from 6×10-5 % to 83.3 %, reflecting the variety of ecosystems that exist in Chile. The database has the potential to inform and test current models that predict SOC stocks and dynamics at larger spatial scales, thus enabling benefits from the richness of geochemical, topographic and climatic variability in Chile. The database is freely available to registered users at https://doi.org/10.17605/OSF.IO/NMYS3 (Pfeiffer et al., 2019b) under the Creative Commons Attribution 4.0 International Public License.
Marco Pfeiffer; José Padarian; Rodrigo Osorio; Nelson Bustamante; Guillermo Federico Olmedo; Mario Guevara; Felipe Aburto; Francisco Albornoz; Monica Antilén; Elías Araya; Eduardo Arellano; Maialen Barret; Juan Barrera; Pascal Boeckx; Margarita Briceño; Sally Bunning; Lea Cabrol; Manuel Casanova; Pablo Cornejo; Fabio Corradini; Gustavo Curaqueo; Sebastian Doetterl; Paola Duran; Mauricio Escudey; Angelina Espinoza; Samuel Francke; Juan Pablo Fuentes; Marcel Fuentes; Gonzalo Gajardo; Rafael García; Audrey Gallaud; Mauricio Galleguillos; Andrés Gomez; Marcela Hidalgo; Jorge Ivelic-Sáez; Lwando Mashalaba; Francisco Matus; Francisco Meza; Maria De La Luz Mora; Jorge Mora; Cristina Muñoz; Pablo Norambuena; Carolina Olivera; Carlos Ovalle; Marcelo Panichini; Aníbal Pauchard; Jorge F. Pérez-Quezada; Sergio Radic; José Ramirez; Nicolás Riveras; Germán Ruiz; Osvaldo Salazar; Iván Salgado; Oscar Seguel; Maria Sepúlveda; Carlos Sierra; Yasna Tapia; Francisco Tapia; Balfredo Toledo; José Miguel Torrico; Susana Valle; Ronald Vargas; Michael Wolff; Erick Zagal. CHLSOC: the Chilean Soil Organic Carbon database, a multi-institutional collaborative effort. Earth System Science Data 2020, 12, 457 -468.
AMA StyleMarco Pfeiffer, José Padarian, Rodrigo Osorio, Nelson Bustamante, Guillermo Federico Olmedo, Mario Guevara, Felipe Aburto, Francisco Albornoz, Monica Antilén, Elías Araya, Eduardo Arellano, Maialen Barret, Juan Barrera, Pascal Boeckx, Margarita Briceño, Sally Bunning, Lea Cabrol, Manuel Casanova, Pablo Cornejo, Fabio Corradini, Gustavo Curaqueo, Sebastian Doetterl, Paola Duran, Mauricio Escudey, Angelina Espinoza, Samuel Francke, Juan Pablo Fuentes, Marcel Fuentes, Gonzalo Gajardo, Rafael García, Audrey Gallaud, Mauricio Galleguillos, Andrés Gomez, Marcela Hidalgo, Jorge Ivelic-Sáez, Lwando Mashalaba, Francisco Matus, Francisco Meza, Maria De La Luz Mora, Jorge Mora, Cristina Muñoz, Pablo Norambuena, Carolina Olivera, Carlos Ovalle, Marcelo Panichini, Aníbal Pauchard, Jorge F. Pérez-Quezada, Sergio Radic, José Ramirez, Nicolás Riveras, Germán Ruiz, Osvaldo Salazar, Iván Salgado, Oscar Seguel, Maria Sepúlveda, Carlos Sierra, Yasna Tapia, Francisco Tapia, Balfredo Toledo, José Miguel Torrico, Susana Valle, Ronald Vargas, Michael Wolff, Erick Zagal. CHLSOC: the Chilean Soil Organic Carbon database, a multi-institutional collaborative effort. Earth System Science Data. 2020; 12 (1):457-468.
Chicago/Turabian StyleMarco Pfeiffer; José Padarian; Rodrigo Osorio; Nelson Bustamante; Guillermo Federico Olmedo; Mario Guevara; Felipe Aburto; Francisco Albornoz; Monica Antilén; Elías Araya; Eduardo Arellano; Maialen Barret; Juan Barrera; Pascal Boeckx; Margarita Briceño; Sally Bunning; Lea Cabrol; Manuel Casanova; Pablo Cornejo; Fabio Corradini; Gustavo Curaqueo; Sebastian Doetterl; Paola Duran; Mauricio Escudey; Angelina Espinoza; Samuel Francke; Juan Pablo Fuentes; Marcel Fuentes; Gonzalo Gajardo; Rafael García; Audrey Gallaud; Mauricio Galleguillos; Andrés Gomez; Marcela Hidalgo; Jorge Ivelic-Sáez; Lwando Mashalaba; Francisco Matus; Francisco Meza; Maria De La Luz Mora; Jorge Mora; Cristina Muñoz; Pablo Norambuena; Carolina Olivera; Carlos Ovalle; Marcelo Panichini; Aníbal Pauchard; Jorge F. Pérez-Quezada; Sergio Radic; José Ramirez; Nicolás Riveras; Germán Ruiz; Osvaldo Salazar; Iván Salgado; Oscar Seguel; Maria Sepúlveda; Carlos Sierra; Yasna Tapia; Francisco Tapia; Balfredo Toledo; José Miguel Torrico; Susana Valle; Ronald Vargas; Michael Wolff; Erick Zagal. 2020. "CHLSOC: the Chilean Soil Organic Carbon database, a multi-institutional collaborative effort." Earth System Science Data 12, no. 1: 457-468.