This page has only limited features, please log in for full access.

Unclaimed
Inga Eichhorn
Institute of Microbiology and Epizootics, Department of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 30 July 2020 in Microorganisms
Reads 0
Downloads 0

The mechanisms of linezolid resistance among 13 E. faecalis and 6 E. faecium isolates, recovered from six Spanish hospitals during 2017–2018, were investigated. The presence of acquired linezolid resistance genes and mutations in 23S rDNA and in genes encoding for ribosomal proteins was analyzed by PCR and amplicon sequencing. Moreover, the susceptibility to 18 antimicrobial agents was investigated, and the respective molecular background was elucidated by PCR-amplicon sequencing and whole genome sequencing. The transferability of the linezolid resistance genes was evaluated by filter-mating experiments. The optrA gene was detected in all 13 E. faecalis isolates; and one optrA-positive isolate also carried the recently described cfr(D) gene. Moreover, one E. faecalis isolate displayed the nucleotide mutation G2576T in the 23S rDNA. This mutation was also present in all six E. faecium isolates. All linezolid-resistant enterococci showed a multiresistance phenotype and harbored several antimicrobial resistance genes, as well as many virulence determinants. The fexA gene was located upstream of the optrA gene in 12 of the E. faecalis isolates. Moreover, an erm(A)-like gene was located downstream of optrA in two isolates recovered from the same hospital. The optrA gene was transferable in all but one E. faecalis isolates, in all cases along with the fexA gene. The cfr(D) gene was not transferable. The presence of optrA and mutations in the 23S rDNA are the main mechanisms of linezolid resistance among E. faecalis and E. faecium, respectively. We report the first description of the cfr(D) gene in E. faecalis. The presence of the optrA and cfr(D) genes in Spanish hospitals is a public health concern.

ACS Style

Laura Ruiz-Ripa; Andrea T. Feßler; Dennis Hanke; Inga Eichhorn; José Manuel Azcona-Gutiérrez; Mar Olga Pérez-Moreno; Cristina Seral; Carmen Aspiroz; Carla Andrea Alonso; Luis Torres; Juan-Ignacio Alós; Stefan Schwarz; Carmen Torres. Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E.faecalis. Microorganisms 2020, 8, 1155 .

AMA Style

Laura Ruiz-Ripa, Andrea T. Feßler, Dennis Hanke, Inga Eichhorn, José Manuel Azcona-Gutiérrez, Mar Olga Pérez-Moreno, Cristina Seral, Carmen Aspiroz, Carla Andrea Alonso, Luis Torres, Juan-Ignacio Alós, Stefan Schwarz, Carmen Torres. Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E.faecalis. Microorganisms. 2020; 8 (8):1155.

Chicago/Turabian Style

Laura Ruiz-Ripa; Andrea T. Feßler; Dennis Hanke; Inga Eichhorn; José Manuel Azcona-Gutiérrez; Mar Olga Pérez-Moreno; Cristina Seral; Carmen Aspiroz; Carla Andrea Alonso; Luis Torres; Juan-Ignacio Alós; Stefan Schwarz; Carmen Torres. 2020. "Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E.faecalis." Microorganisms 8, no. 8: 1155.

Journal article
Published: 21 June 2020 in Heliyon
Reads 0
Downloads 0

Surface disinfectants are regularly used in prophylactic and infection control measures. Concern has been raised whether residues of sub-inhibitory disinfectant concentrations may constitute a selective pressure and could contribute to the development of strains which are tolerant and/or resistant to biocides including antibiotics. The current study investigated whether Staphylococcus (S.) aureus ATCC® 29213™ and ATCC® 6538™ would change their growth characteristics and antimicrobial susceptibility profiles after prolonged treatment with sub-inhibitory concentrations of sodium hypochlorite (NaOCl). NaOCl is a fast-acting disinfectant with a broad-spectrum activity, inexpensive and widely used in healthcare and the food production industry. Minimum inhibitory concentration (MIC) for NaOCl was determined by broth macrodilution according to the guidelines for disinfectant efficacy testing provided by the German Veterinary Medical Society. Serial passages after 24 h and 72 h, respectively, in defined sub-inhibitory concentrations of NaOCl resulted in a number of phenotypic variants. Two of these variants, derived from S. aureus ATCC® 29213™, showed elevated MICs of oxacillin and were considered as in vitro-generated borderline oxacillin-resistant S. aureus (BORSA). Transmission electron microscopy revealed a significantly thickened cell wall in these isolates, a phenomenon that has also been described for Listeria monocytogenes after low-level exposure to NaOCl. Whole genome sequencing revealed an early stop codon in the gene coding for the GdpP protein and thereby abolishing the function of this gene. GdpP represents a phosphodiesterase that regulates gene expression, and loss of function of the GdpP protein has been described in association with borderline oxacillin resistance. Our findings suggest that a mutation in the GdpP protein gene and morphological changes of the cell wall were induced by repeated exposure to sub-lethal NaOCl concentrations, and most likely accounted for a BORSA phenotype in two variants derived from S. aureus ATCC® 29213™.

ACS Style

Stephanie Speck; Cindy Wenke; Andrea T. Feßler; Johannes Kacza; Franziska Geber; Anissa D. Scholtzek; Dennis Hanke; Inga Eichhorn; Stefan Schwarz; Maciej Rosolowski; Uwe Truyen. Borderline resistance to oxacillin in Staphylococcus aureus after treatment with sub-lethal sodium hypochlorite concentrations. Heliyon 2020, 6, e04070 .

AMA Style

Stephanie Speck, Cindy Wenke, Andrea T. Feßler, Johannes Kacza, Franziska Geber, Anissa D. Scholtzek, Dennis Hanke, Inga Eichhorn, Stefan Schwarz, Maciej Rosolowski, Uwe Truyen. Borderline resistance to oxacillin in Staphylococcus aureus after treatment with sub-lethal sodium hypochlorite concentrations. Heliyon. 2020; 6 (6):e04070.

Chicago/Turabian Style

Stephanie Speck; Cindy Wenke; Andrea T. Feßler; Johannes Kacza; Franziska Geber; Anissa D. Scholtzek; Dennis Hanke; Inga Eichhorn; Stefan Schwarz; Maciej Rosolowski; Uwe Truyen. 2020. "Borderline resistance to oxacillin in Staphylococcus aureus after treatment with sub-lethal sodium hypochlorite concentrations." Heliyon 6, no. 6: e04070.

Short communication
Published: 26 December 2019 in Journal of Global Antimicrobial Resistance
Reads 0
Downloads 0

Two linezolid-resistant Enterococcus faecium isolates, C10004 and C10009, were recovered from air samples of a Spanish swine farm and comprehensively characterized. Detection of linezolid resistance mechanisms (mutations and acquisition of resistance genes) was performed by PCR/sequencing. Isolates were characterized by multilocus sequence typing (MLST), antimicrobial susceptibility testing, detection of antimicrobial resistance and virulence genes, and analysis of the genetic environment of the linezolid resistance genes. The characterization of isolate C10009 was performed by Whole-Genome-Sequencing and of isolate C10004 by PCR and amplicon sequencing, where applicable. Conjugation experiments to assess the transferability of the optrA and poxtA genes implicated in linezolid resistance were performed. The linezolid-resistant E. faecium isolates C10004 and C10009, assigned to ST128 and ST437, respectively, harbored the optrA and poxtA genes. Neither mutations in the 23S rRNA nor in the genes for the ribosomal proteins L3, L4 and L22 were detected. C10004 and C10009 carried fourteen and thirteen antimicrobial resistance genes, respectively. The sequence alignment indicated that the genetic environment of the poxtA gene was identical in both isolates, with a downstream-located fexB gene. The poxtA gene was transferred by conjugation together with the fexB gene, and also with tet(M) and tet(L) in the case of isolate C10004. The optrA gene could not be transferred. This is the first report of the poxtA gene in Spain. The presence of poxtA- and optrA-carrying E. faecium isolates in air samples represents a public health concern, indicating an involvement of swine farms in the spread of linezolid-resistant bacteria.

ACS Style

Laura Ruiz-Ripa; Andrea T. Feßler; Dennis Hanke; Susana Sanz; Carmen Olarte; Inga Eichhorn; Stefan Schwarz; Carmen Torres. Detection of poxtA- and optrA-carrying E. faecium isolates in air samples of a Spanish swine farm. Journal of Global Antimicrobial Resistance 2019, 22, 28 -31.

AMA Style

Laura Ruiz-Ripa, Andrea T. Feßler, Dennis Hanke, Susana Sanz, Carmen Olarte, Inga Eichhorn, Stefan Schwarz, Carmen Torres. Detection of poxtA- and optrA-carrying E. faecium isolates in air samples of a Spanish swine farm. Journal of Global Antimicrobial Resistance. 2019; 22 ():28-31.

Chicago/Turabian Style

Laura Ruiz-Ripa; Andrea T. Feßler; Dennis Hanke; Susana Sanz; Carmen Olarte; Inga Eichhorn; Stefan Schwarz; Carmen Torres. 2019. "Detection of poxtA- and optrA-carrying E. faecium isolates in air samples of a Spanish swine farm." Journal of Global Antimicrobial Resistance 22, no. : 28-31.

Journal article
Published: 13 September 2019 in Toxins
Reads 0
Downloads 0

The detection of borderline oxacillin-resistant Staphylococcus aureus (BORSA) represents a challenge to both, veterinary and human laboratories. Between 2015 and 2017, 19 equine S. aureus with elevated minimal inhibitory concentrations for oxacillin were detected in routine diagnostics. The aim of this study was to characterize these isolates to identify factors possibly associated with the BORSA phenotype. All S. aureus were subjected to antimicrobial susceptibility testing and whole genome sequencing (WGS). A quantifiable β-lactamase activity assay was performed for a representative subset of 13 isolates. The WGS data analysis of the 19 BORSA isolates identified two different genomic lineages, sequence type (ST) 1 and ST1660. The core genome multilocus sequence typing (cgMLST) revealed a close relatedness of all isolates belonging to either ST1 or ST1660. The WGS analysis identified the resistance genes aadD, dfrG, tet(L), and/or blaZ and aacA-aphD. Phenotypic resistance to penicillins, aminoglycosides, tetracyclines, fluoroquinolones and sulfamethoxazole/trimethoprim was observed in the respective isolates. For the penicillin-binding proteins 1–4, amino acid substitutions were predicted using WGS data. Since neither transglycosylase nor transpeptidase domains were affected, these alterations might not explain the BORSA phenotype. Moreover, β-lactamase activity was found to be associated with an inducible blaZ gene. The lineage-specific differences regarding the expression profiles were noted.

ACS Style

Anissa D. Scholtzek; Dennis Hanke; Birgit Walther; Inga Eichhorn; Sabita D. Stöckle; Katja-Sophia Klein; Heidrun Gehlen; Antina Lübke-Becker; Stefan Schwarz; Andrea T. Feßler; Lübke- Becker. Molecular Characterization of Equine Staphylococcus aureus Isolates Exhibiting Reduced Oxacillin Susceptibility. Toxins 2019, 11, 535 .

AMA Style

Anissa D. Scholtzek, Dennis Hanke, Birgit Walther, Inga Eichhorn, Sabita D. Stöckle, Katja-Sophia Klein, Heidrun Gehlen, Antina Lübke-Becker, Stefan Schwarz, Andrea T. Feßler, Lübke- Becker. Molecular Characterization of Equine Staphylococcus aureus Isolates Exhibiting Reduced Oxacillin Susceptibility. Toxins. 2019; 11 (9):535.

Chicago/Turabian Style

Anissa D. Scholtzek; Dennis Hanke; Birgit Walther; Inga Eichhorn; Sabita D. Stöckle; Katja-Sophia Klein; Heidrun Gehlen; Antina Lübke-Becker; Stefan Schwarz; Andrea T. Feßler; Lübke- Becker. 2019. "Molecular Characterization of Equine Staphylococcus aureus Isolates Exhibiting Reduced Oxacillin Susceptibility." Toxins 11, no. 9: 535.

Research paper
Published: 01 January 2019 in Virulence
Reads 0
Downloads 0

Streptococcus canis is a zoonotic agent that causes serious invasive diseases in domestic animals and humans, but knowledge about its pathogenic potential and underlying virulence mechanisms is limited. Here, we report on the ability of certain S. canis isolates to form large bacterial aggregates when grown in liquid broth. Bacterial aggregation was attributed to the presence and the self-binding activity of SCM, the M protein of S. canis, as evaluated by bacterial sedimentation assays, immunofluorescence- and electron microscopic approaches. Using a variety of truncated recombinant SCM fragments, we demonstrated that homophilic SCM interactions occur via the N-terminal, but not the C-terminal part, of the mature M protein. Interestingly, when incubated in human plasma, SCM forms soluble protein complexes comprising its known ligands, immunoglobulin G (IgG) and plasminogen (Plg). Co-incubation studies with purified host proteins revealed that SCM-mediated complex formation is based on the interaction of SCM with itself and with IgG, but not with Plg or fibrinogen (Fbg), well-established constituents of M protein-mediated protein complexes in human-associated streptococci. Notably, these soluble, SCM-mediated plasma complexes harbored complement factor C1q, which can induce complement breakdown in the periphery and therefore represent another immune evasion mechanism of SCM.

ACS Style

Andreas Nerlich; Antje-Maria Lapschies; Thomas P. Kohler; Ingrid Cornax; Inga Eichhorn; Oliver Goldmann; Petra Krienke; Simone Bergmann; Victor Nizet; Sven Hammerschmidt; Manfred Rohde; Marcus Fulde. Homophilic protein interactions facilitate bacterial aggregation and IgG-dependent complex formation by the Streptococcus canis M protein SCM. Virulence 2019, 10, 194 -206.

AMA Style

Andreas Nerlich, Antje-Maria Lapschies, Thomas P. Kohler, Ingrid Cornax, Inga Eichhorn, Oliver Goldmann, Petra Krienke, Simone Bergmann, Victor Nizet, Sven Hammerschmidt, Manfred Rohde, Marcus Fulde. Homophilic protein interactions facilitate bacterial aggregation and IgG-dependent complex formation by the Streptococcus canis M protein SCM. Virulence. 2019; 10 (1):194-206.

Chicago/Turabian Style

Andreas Nerlich; Antje-Maria Lapschies; Thomas P. Kohler; Ingrid Cornax; Inga Eichhorn; Oliver Goldmann; Petra Krienke; Simone Bergmann; Victor Nizet; Sven Hammerschmidt; Manfred Rohde; Marcus Fulde. 2019. "Homophilic protein interactions facilitate bacterial aggregation and IgG-dependent complex formation by the Streptococcus canis M protein SCM." Virulence 10, no. 1: 194-206.

Journal article
Published: 08 October 2018 in Gut Pathogens
Reads 0
Downloads 0

In the current study, nine foodborne “Locus of Enterocyte Effacement” (LEE)-negative Shiga toxin-producing Escherichia coli (STEC) strains were selected for whole genome sequencing and analysis for yet unknown genetic elements within the already known LEE integration sites selC, pheU and pheV. Foreign DNA ranging in size from 3.4 to 57 kbp was detected and further analyzed. Five STEC strains contained an insertion of foreign DNA adjacent to the selC tRNA gene and five and seven strains contained foreign DNA adjacent to the pheU and pheV tRNA genes, respectively. We characterized the foreign DNA insertion associated with selC (STEC O91:H21 strain 17584/1), pheU (STEC O8:H4 strain RF1a and O55:Hnt strain K30) and pheV (STEC O91:H21 strain 17584/1 and O113:H21 strain TS18/08) as examples. In total, 293 open reading frames partially encoding putative virulence factors such as TonB-dependent receptors, DNA helicases, a hemolysin activator protein precursor, antigen 43, anti-restriction protein KlcA, ShiA, and phosphoethanolamine transferases were detected. A virulence type IV toxin-antitoxin system was detected in three strains. Additionally, the ato system was found in one strain. In strain 17584/1 we were able to define a new genomic island which we designated GIselC17584/1. The island contained integrases and mobile elements in addition to genes for increased fitness and those playing a putative role in pathogenicity. The data presented highlight the important role of the three tRNAs selC, pheU, and pheV for the genomic flexibility of E. coli.

ACS Style

Nadja Saile; Elisabeth Schuh; Torsten Semmler; Inga Eichhorn; Lothar H. Wieler; Andreas Bauwens; Herbert Schmidt. Determination of virulence and fitness genes associated with the pheU, pheV and selC integration sites of LEE-negative food-borne Shiga toxin-producing Escherichia coli strains. Gut Pathogens 2018, 10, 1 -12.

AMA Style

Nadja Saile, Elisabeth Schuh, Torsten Semmler, Inga Eichhorn, Lothar H. Wieler, Andreas Bauwens, Herbert Schmidt. Determination of virulence and fitness genes associated with the pheU, pheV and selC integration sites of LEE-negative food-borne Shiga toxin-producing Escherichia coli strains. Gut Pathogens. 2018; 10 (1):1-12.

Chicago/Turabian Style

Nadja Saile; Elisabeth Schuh; Torsten Semmler; Inga Eichhorn; Lothar H. Wieler; Andreas Bauwens; Herbert Schmidt. 2018. "Determination of virulence and fitness genes associated with the pheU, pheV and selC integration sites of LEE-negative food-borne Shiga toxin-producing Escherichia coli strains." Gut Pathogens 10, no. 1: 1-12.

Journal article
Published: 01 October 2018 in International Journal of Medical Microbiology
Reads 0
Downloads 0

Bacteriophages play an important role in the evolution of bacterial pathogens. A phage-mediated transfer of stx-genes to atypical enteropathogenic E. coli (aEPEC) which are prevalent in different hosts, would convert them to enterohemorrhagic E. coli (EHEC). We decided to confirm this hypothesis experimentally to provide conclusive evidence that aEPEC isolated from different mammalian hosts are indeed progenitors of typical EHEC which gain the ability to produce Shiga-Toxin by lysogeny with stx-converting bacteriophages, utilizing the model phage Φ3538 Δstx2::cat. We applied a modified in vitro plaque-assay, using a high titer of a bacteriophage carrying a deletion in the stx2 gene (Φ3538 Δstx2::cat) to increase the detection of lysogenic conversion events. Three wild-type aEPEC strains were chosen as acceptor strains: the murine aEPEC-strain IMT14505 (sequence type (ST)28, serotype Ont:H6), isolated from a striped field mouse (Apodemus agrarius) in the surrounding of a cattle shed, and the human aEPEC-strain 910#00 (ST28, Ont:H6). The close genomic relationship of both strains implies a high zoonotic potential. A third strain, the bovine aEPEC IMT19981, was of serotype O26:H11 and ST21 (STC29). All three aEPEC were successfully lysogenized with phage Φ3538 Δstx2::cat. Integration of the bacteriophage DNA into the aEPEC host genomes was confirmed by amplification of chloramphenicol transferase (cat) marker gene and by Southern-Blot hybridization. Analysis of the whole genome sequence of each of the three lysogens showed that the bacteriophage was integrated into the known tRNA integration site argW, which is highly variable among E. coli. In conclusion, the successful lysogenic conversion of aEPEC with a stx-phage in vitro underlines the important role of aEPEC as progenitors of EHEC. Given the high prevalence and the wide host range of aEPEC acceptors, their high risk of zoonotic transmission should be recognized in infection control measures.

ACS Style

Inga Eichhorn; Katrin Heidemanns; Rainer G. Ulrich; Herbert Schmidt; Torsten Semmler; Angelika Fruth; Astrid Bethe; David Goulding; Derek Pickard; Helge Karch; Lothar H. Wieler. Lysogenic conversion of atypical enteropathogenic Escherichia coli (aEPEC) from human, murine, and bovine origin with bacteriophage Φ3538 Δstx::cat proves their enterohemorrhagic E. coli (EHEC) progeny. International Journal of Medical Microbiology 2018, 308, 890 -898.

AMA Style

Inga Eichhorn, Katrin Heidemanns, Rainer G. Ulrich, Herbert Schmidt, Torsten Semmler, Angelika Fruth, Astrid Bethe, David Goulding, Derek Pickard, Helge Karch, Lothar H. Wieler. Lysogenic conversion of atypical enteropathogenic Escherichia coli (aEPEC) from human, murine, and bovine origin with bacteriophage Φ3538 Δstx::cat proves their enterohemorrhagic E. coli (EHEC) progeny. International Journal of Medical Microbiology. 2018; 308 (7):890-898.

Chicago/Turabian Style

Inga Eichhorn; Katrin Heidemanns; Rainer G. Ulrich; Herbert Schmidt; Torsten Semmler; Angelika Fruth; Astrid Bethe; David Goulding; Derek Pickard; Helge Karch; Lothar H. Wieler. 2018. "Lysogenic conversion of atypical enteropathogenic Escherichia coli (aEPEC) from human, murine, and bovine origin with bacteriophage Φ3538 Δstx::cat proves their enterohemorrhagic E. coli (EHEC) progeny." International Journal of Medical Microbiology 308, no. 7: 890-898.

Journal article
Published: 10 August 2018 in International Journal of Medical Microbiology
Reads 0
Downloads 0

Enterohemorrhagic Escherichia coli (EHEC) are a cause of bloody diarrhea, hemorrhagic colitis (HC) and the potentially fatal hemolytic uremic syndrome (HUS). While O157:H7 is the dominant EHEC serotype, non-O157 EHEC have emerged as serious causes of disease. In Germany, the most important non-O157 O-serogroups causing one third of EHEC infections, including diarrhea as well as HUS, are O26, O103, O111 and O145. Interestingly, we identified EHEC O-serogroups O26 and O111 in one single sequence type complex, STC29, that also harbours atypical enteropathogenic E. coli (aEPEC). aEPEC differ from typical EHEC merely in the absence of stx-genes. These findings inspired us to unravel a putative microevolutionary scenario of these non-O157 EHEC by whole genome analyses. Analysis of single nucleotide polymorphisms (SNPs) of the maximum common genome (MCG) of 20 aEPEC (11 human/ 9 bovine) and 79 EHEC (42 human/ 36 bovine/ 1 food source) of STC29 identified three distinct clusters: Cluster 1 harboured strains of O-serogroup O111, the central Cluster 2 harboured only O26 aEPEC strains, while the more heterogeneous Cluster 3 contained both EHEC and aEPEC strains of O-serogroup O26. Further combined analyses of accessory virulence associated genes (VAGs) and insertion sites for mobile genetic elements suggested a parallel evolution of the MCG and the acquisition of virulence genes. The resulting microevolutionary model suggests the development of two distinct EHEC lineages from one common aEPEC ancestor of ST29 by lysogenic conversion with stx-converting bacteriophages, independent of the host species the strains had been isolated from. In conclusion, our cumulative data indicate that EHEC of O-serogroups O26 and O111 of STC29 originate from a common aEPEC ancestor and are bona fide zoonotic agents. The role of aEPEC in the emergence of O26 and O111 EHEC should be considered for infection control measures to prevent possible lysogenic conversion with stx-converting bacteriophages as major vehicle driving the emergence of EHEC lineages with direct Public Health consequences.

ACS Style

Inga Eichhorn; Torsten Semmler; Alexander Mellmann; Derek Pickard; Muna F. Anjum; Angelika Fruth; Helge Karch; Lothar H. Wieler. Microevolution of epidemiological highly relevant non-O157 enterohemorrhagic Escherichia coli of serogroups O26 and O111. International Journal of Medical Microbiology 2018, 308, 1085 -1095.

AMA Style

Inga Eichhorn, Torsten Semmler, Alexander Mellmann, Derek Pickard, Muna F. Anjum, Angelika Fruth, Helge Karch, Lothar H. Wieler. Microevolution of epidemiological highly relevant non-O157 enterohemorrhagic Escherichia coli of serogroups O26 and O111. International Journal of Medical Microbiology. 2018; 308 (8):1085-1095.

Chicago/Turabian Style

Inga Eichhorn; Torsten Semmler; Alexander Mellmann; Derek Pickard; Muna F. Anjum; Angelika Fruth; Helge Karch; Lothar H. Wieler. 2018. "Microevolution of epidemiological highly relevant non-O157 enterohemorrhagic Escherichia coli of serogroups O26 and O111." International Journal of Medical Microbiology 308, no. 8: 1085-1095.

Journal article
Published: 14 November 2017 in Acta Veterinaria Scandinavica
Reads 0
Downloads 0

The aim of the present study was to investigate the genetic relatedness and the antimicrobial resistance profiles of a collection of Austrian Streptococcus pneumoniae isolates from companion animals and horses. A total of 12 non-repetitive isolates presumptively identified as S. pneumoniae were obtained during routinely diagnostic activities between March 2009 and January 2017. Isolates were confirmed as S. pneumoniae by bile solubility and optochin susceptibility testing, matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) mass spectrometry and sequence analysis of a part recA and the 16S rRNA genes. Isolates were further characterized by pneumolysin polymerase chain reaction (PCR) and genotyped by multilocus sequence typing (MLST). Antimicrobial susceptibility testing was performed and resistance genes were detected by specific PCR assays. All isolates were serotyped. Four sequence types (ST) (ST36, ST3546, ST6934 and ST6937) and four serotypes (3, 19A, 19F and 23F) were detected. Two isolates from twelve displayed a multidrug-resistance pheno- and genotype. This study represents the first comprehensive investigation on characteristics of S. pneumoniae isolates recovered from Austrian companion animals and horses. The obtained results indicate that common human sero- (23F) and sequence type (ST36) implicated in causing invasive pneumococcal disease (IPD) may circulate in dogs. Isolates obtained from other examined animals seem to be host-adapted.

ACS Style

Maximilian Ginders; Michael Leschnik; Frank Künzel; Doris Kampner; Claudia Mikula; Georg Steindl; Inga Eichhorn; Andrea T. Feßler; Stefan Schwarz; Joachim Spergser; Igor Loncaric. Characterization of Streptococcus pneumoniae isolates from Austrian companion animals and horses. Acta Veterinaria Scandinavica 2017, 59, 79 -79.

AMA Style

Maximilian Ginders, Michael Leschnik, Frank Künzel, Doris Kampner, Claudia Mikula, Georg Steindl, Inga Eichhorn, Andrea T. Feßler, Stefan Schwarz, Joachim Spergser, Igor Loncaric. Characterization of Streptococcus pneumoniae isolates from Austrian companion animals and horses. Acta Veterinaria Scandinavica. 2017; 59 (1):79-79.

Chicago/Turabian Style

Maximilian Ginders; Michael Leschnik; Frank Künzel; Doris Kampner; Claudia Mikula; Georg Steindl; Inga Eichhorn; Andrea T. Feßler; Stefan Schwarz; Joachim Spergser; Igor Loncaric. 2017. "Characterization of Streptococcus pneumoniae isolates from Austrian companion animals and horses." Acta Veterinaria Scandinavica 59, no. 1: 79-79.

Journal article
Published: 01 October 2017 in Infection, Genetics and Evolution
Reads 0
Downloads 0

Clostridium (C.) chauvoei is a Gram-positive, spore forming, anaerobic bacterium. It causes black leg in ruminants, a typically fatal histotoxic myonecrosis. High quality circular genome sequences were generated for the C. chauvoei type strain DSM 7528(T) (ATCC 10092(T)) and a field strain 12S0467 isolated in Germany. The origin of replication (oriC) was comparable to that of Bacillus subtilis in structure with two regions containing DnaA boxes. Similar prophages were identified in the genomes of both C. chauvoei strains which also harbored hemolysin and bacterial spore formation genes. A CRISPR type I-B system with limited variations in the repeat number was identified. Sporulation and germination process related genes were homologous to that of the Clostridia cluster I group but novel variations for regulatory genes were identified indicative for strain specific control of regulatory events. Phylogenomics showed a higher relatedness to C. septicum than to other so far sequenced genomes of species belonging to the genus Clostridium. Comparative genome analysis of three C. chauvoei circular genome sequences revealed the presence of few inversions and translocations in locally collinear blocks (LCBs). The species genome also shows a large number of genes involved in proteolysis, genes for glycosyl hydrolases and metal iron transportation genes which are presumably involved in virulence and survival in the host. Three conserved flagellar genes (fliC) were identified in each of the circular genomes. In conclusion this is the first comparative analysis of circular genomes for the species C. chauvoei, enabling insights into genome composition and virulence factor variation.

ACS Style

Prasad Thomas; Torsten Semmler; Inga Eichhorn; Antina Lübke-Becker; Christiane Werckenthin; Mostafa Y. Abdel-Glil; Lothar H. Wieler; Heinrich Neubauer; Christian Seyboldt. First report of two complete Clostridium chauvoei genome sequences and detailed in silico genome analysis. Infection, Genetics and Evolution 2017, 54, 287 -298.

AMA Style

Prasad Thomas, Torsten Semmler, Inga Eichhorn, Antina Lübke-Becker, Christiane Werckenthin, Mostafa Y. Abdel-Glil, Lothar H. Wieler, Heinrich Neubauer, Christian Seyboldt. First report of two complete Clostridium chauvoei genome sequences and detailed in silico genome analysis. Infection, Genetics and Evolution. 2017; 54 ():287-298.

Chicago/Turabian Style

Prasad Thomas; Torsten Semmler; Inga Eichhorn; Antina Lübke-Becker; Christiane Werckenthin; Mostafa Y. Abdel-Glil; Lothar H. Wieler; Heinrich Neubauer; Christian Seyboldt. 2017. "First report of two complete Clostridium chauvoei genome sequences and detailed in silico genome analysis." Infection, Genetics and Evolution 54, no. : 287-298.

Research article
Published: 05 April 2017 in Journal of Veterinary Diagnostic Investigation
Reads 0
Downloads 0

We assessed the ability of a commercial DNA microarray to characterize bovine Shiga toxin–producing Escherichia coli (STEC) isolates and evaluated the results using in silico hybridization of the microarray probes within whole genome sequencing scaffolds. From a total of 69,954 reactions (393 probes with 178 isolates), 68,706 (98.2%) gave identical results by DNA microarray and in silico probe hybridization. Results were more congruent when detecting the genoserotype (209 differing results from 19,758 in total; 1.1%) or antimicrobial resistance genes (AMRGs; 141 of 26,878; 0.5%) than when detecting virulence-associated genes (VAGs; 876 of 22,072; 4.0%). Owing to the limited coverage of O-antigens by the microarray, only 37.2% of the isolates could be genoserotyped. However, the microarray proved suitable to rapidly screen bovine STEC strains for the occurrence of high numbers of VAGs and AMRGs and is suitable for molecular surveillance workflows.

ACS Style

Stefanie A. Barth; Christian Menge; Inga Eichhorn; Lutz Geue; Torsten Semmler; Derek Pickard. Evaluation of applicability of DNA microarray–based characterization of bovine Shiga toxin–producing Escherichia coli isolates using whole genome sequence analysis. Journal of Veterinary Diagnostic Investigation 2017, 29, 721 -724.

AMA Style

Stefanie A. Barth, Christian Menge, Inga Eichhorn, Lutz Geue, Torsten Semmler, Derek Pickard. Evaluation of applicability of DNA microarray–based characterization of bovine Shiga toxin–producing Escherichia coli isolates using whole genome sequence analysis. Journal of Veterinary Diagnostic Investigation. 2017; 29 (5):721-724.

Chicago/Turabian Style

Stefanie A. Barth; Christian Menge; Inga Eichhorn; Lutz Geue; Torsten Semmler; Derek Pickard. 2017. "Evaluation of applicability of DNA microarray–based characterization of bovine Shiga toxin–producing Escherichia coli isolates using whole genome sequence analysis." Journal of Veterinary Diagnostic Investigation 29, no. 5: 721-724.