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Canine innate immune system role in cancer prevention and progression remains poorly understood. It has been revealed that innate immune cells could play a dual role in cancer immunology promoting or inhibiting tumor development and growth. Current immunotherapies target mainly the adaptive anti-tumor response and that may be a reason why they remain ineffective in a majority of patients. It is important to acquire detailed knowledge about innate immune mechanisms to broaden the diagnostic and therapeutic options and employ innate immune cells in anti-cancer therapies. In the present study, 21 female dogs of different breeds and types of spontaneous mammary tumors were investigated. The study aimed to find simple and cheap markers that can be used for preliminary diagnosis, prior to the surgical resection of the tumor. The differences in innate immune cell quantity and function were investigated between female dogs with malignant mammary tumors of epithelial and mesenchymal origin. Flow cytometry was used to evaluate the percentages of CD5+ lymphocytes including CD5low lymphocytes, CD11b integrin expression on leukocytes, phagocytosis, and oxidative burst. The number of CD11b lymphocytes was increased in tumors with epithelial origin compared to the control group. No significant differences were found between the percentages of phagocytic cells neither for granulocytes nor for monocytes. However, the phagocytes of canine patients with tumors of epithelial origin showed increased phagocytosis compared to the control group. The percentages of granulocytes that produced reactive oxygen species (ROS) in response to E.coli and PMA were not altered in patients with malignant tumors compared to control. A statistically significant difference between the number of ROS produced by the single granulocyte was demonstrated only between the group of bitches with epithelial tumors and the control group in case of E. coli stimulation. The obtained results suggest that some innate immune cells may be involved in anti-tumor immune mechanisms and have the potential to be supportive diagnostic markers in canine mammary tumors.
Urszula Lisiecka; Piotr Brodzki; Anna Śmiech; Janusz Kocki; Marcin Czop; Łukasz Adaszek; Stanisław Winiarczyk. Comparative Expression Analysis of Innate Immune Markers and Phagocytic Activity in Peripheral Blood of Dogs with Mammary Tumors. Animals 2021, 11, 2398 .
AMA StyleUrszula Lisiecka, Piotr Brodzki, Anna Śmiech, Janusz Kocki, Marcin Czop, Łukasz Adaszek, Stanisław Winiarczyk. Comparative Expression Analysis of Innate Immune Markers and Phagocytic Activity in Peripheral Blood of Dogs with Mammary Tumors. Animals. 2021; 11 (8):2398.
Chicago/Turabian StyleUrszula Lisiecka; Piotr Brodzki; Anna Śmiech; Janusz Kocki; Marcin Czop; Łukasz Adaszek; Stanisław Winiarczyk. 2021. "Comparative Expression Analysis of Innate Immune Markers and Phagocytic Activity in Peripheral Blood of Dogs with Mammary Tumors." Animals 11, no. 8: 2398.
Atherosclerosis involves an ongoing inflammatory response of the vascular endothelium and vessel wall of the aorta and vein. The pleiotropic effects of statins have been well described in many in vitro and in vivo studies, but these effects are difficult to achieve in clinical practice due to the low bioavailability of statins and their first-pass metabolism in the liver. The aim of this study was to test a vessel wall local drug delivery system (DDS) using PLA microstructures loaded with simvastatin. Wistar rats were fed high cholesterol chow as a model. The rat vessels were chemically injured by repeated injections of perivascular paclitaxel and 5-fluorouracil. The vessels were then cultured and treated by the injection of several concentrations of poly(L,L-lactide) microparticles loaded with the high local HMG-CoA inhibitor simvastatin (0.58 mg/kg) concentration (SVPLA). Histopathological examinations of the harvested vessels and vital organs after 24 h, 7 days and 4 weeks were performed. Microcirculation in mice as an additional test was performed to demonstrate the safety of this approach. A single dose of SVPLA microspheres with an average diameter of 6.4 μm and a drug concentration equal to 8.1% of particles limited the inflammatory reaction of the endothelium and vessel wall and had no influence on microcirculation in vivo or in vitro. A potent pleiotropic (anti-inflammatory) effect of simvastatin after local SVPLA administration was observed. Moreover, significant concentrations of free simvastatin were observed in the vessel wall (compared to the maximum serum level). In addition, it appeared that simvastatin, once locally administered as SVPLA particles, exerted potent pleiotropic effects on chemically injured vessels and presented anti-inflammatory action. Presumably, this effect was due to the high local concentrations of simvastatin. No local or systemic side effects were observed. This approach could be useful for local simvastatin DDSs when high, local drug concentrations are difficult to obtain, or systemic side effects are present.
Piotr Wacinski; Mariusz Gadzinowski; Wojciech Dabrowski; Justyna Szumilo; Jakub Wacinski; Nathalie Oru; Eric Vicaut; Stanislaw Czuczwar; Janusz Kocki; Teresa Basinska; Stanislaw Slomkowski. Anti-Inflammatory Effect of Very High Dose Local Vessel Wall Statin Administration: Poly(L,L-Lactide) Biodegradable Microspheres with Simvastatin for Drug Delivery System (DDS). International Journal of Molecular Sciences 2021, 22, 7486 .
AMA StylePiotr Wacinski, Mariusz Gadzinowski, Wojciech Dabrowski, Justyna Szumilo, Jakub Wacinski, Nathalie Oru, Eric Vicaut, Stanislaw Czuczwar, Janusz Kocki, Teresa Basinska, Stanislaw Slomkowski. Anti-Inflammatory Effect of Very High Dose Local Vessel Wall Statin Administration: Poly(L,L-Lactide) Biodegradable Microspheres with Simvastatin for Drug Delivery System (DDS). International Journal of Molecular Sciences. 2021; 22 (14):7486.
Chicago/Turabian StylePiotr Wacinski; Mariusz Gadzinowski; Wojciech Dabrowski; Justyna Szumilo; Jakub Wacinski; Nathalie Oru; Eric Vicaut; Stanislaw Czuczwar; Janusz Kocki; Teresa Basinska; Stanislaw Slomkowski. 2021. "Anti-Inflammatory Effect of Very High Dose Local Vessel Wall Statin Administration: Poly(L,L-Lactide) Biodegradable Microspheres with Simvastatin for Drug Delivery System (DDS)." International Journal of Molecular Sciences 22, no. 14: 7486.
The innovative Eye Movement Modelling Examples (EMMEs) method can be used in medicine as an educational training tool for the assessment and verification of students and professionals. Our work was intended to analyse the possibility of using eye tracking tools to verify the skills and training of people engaged in laboratory medicine on the example of parasitological diagnostics. Professionally active laboratory diagnosticians working in a multi-profile laboratory (non-parasitological) (n = 16), laboratory diagnosticians no longer working in this profession (n = 10), and medical analyst students (n = 56), participated in the study. The studied group analysed microscopic images of parasitological preparations made with the cellSens Dimension Software (Olympus) system. Eye activity parameters were obtained using a stationary, video-based eye tracker Tobii TX300 which has a 3-ms temporal resolution. Eye movement activity parameters were analysed along with time parameters. The results of our studies have shown that the eye tracking method is a valuable tool for the analysis of parasitological preparations. Detailed quantitative and qualitative analysis confirmed that the EMMEs method may facilitate learning of the correct microscopic image scanning path. The analysis of the results of our studies allows us to conclude that the EMMEs method may be a valuable tool in the preparation of teaching materials in virtual microscopy. These teaching materials generated with the use of eye tracking, prepared by experienced professionals in the field of laboratory medicine, can be used during various training, simulations and courses in medical parasitology and contribute to the verification of education results, professional skills, and elimination of errors in parasitological diagnostics.
Przemysław Kołodziej; Wioletta Tuszyńska-Bogucka; Mariusz Dzieńkowski; Jacek Bogucki; Janusz Kocki; Marek Milosz; Marcin Kocki; Patrycja Reszka; Wojciech Kocki; Anna Bogucka-Kocka. Eye Tracking—An Innovative Tool in Medical Parasitology. Journal of Clinical Medicine 2021, 10, 2989 .
AMA StylePrzemysław Kołodziej, Wioletta Tuszyńska-Bogucka, Mariusz Dzieńkowski, Jacek Bogucki, Janusz Kocki, Marek Milosz, Marcin Kocki, Patrycja Reszka, Wojciech Kocki, Anna Bogucka-Kocka. Eye Tracking—An Innovative Tool in Medical Parasitology. Journal of Clinical Medicine. 2021; 10 (13):2989.
Chicago/Turabian StylePrzemysław Kołodziej; Wioletta Tuszyńska-Bogucka; Mariusz Dzieńkowski; Jacek Bogucki; Janusz Kocki; Marek Milosz; Marcin Kocki; Patrycja Reszka; Wojciech Kocki; Anna Bogucka-Kocka. 2021. "Eye Tracking—An Innovative Tool in Medical Parasitology." Journal of Clinical Medicine 10, no. 13: 2989.
Several human tissues are investigated in studies of molecular biomarkers associated with diseases development. Special attention is focused on the blood and its components due to combining abundant information about systemic responses to pathological processes as well as high accessibility. In the current study, transcriptome profiles of peripheral blood mononuclear cells (PBMCs) were used to compare differentially expressed genes between patients with lower extremities arterial disease (LEAD), abdominal aortic aneurysm (AAA) and chronic venous disease (CVD). Gene expression patterns were generated using the Ion S5XL next-generation sequencing platform and were analyzed using DESeq2 and UVE-PLS methods implemented in R programming software. In direct pairwise analysis, 21, 58 and 10 differentially expressed genes were selected from the comparison of LEAD vs. AAA, LEAD vs. CVD and AAA vs. CVD patient groups, respectively. Relationships between expression of dysregulated genes and age, body mass index, creatinine levels, hypertension and medication were identified using Spearman rank correlation test and two-sided Mann–Whitney U test. The functional analysis, performed using DAVID website tool, provides potential implications of selected genes in pathological processes underlying diseases studied. Presented research provides new insight into differences of pathogenesis in LEAD, AAA and CVD, and selected genes could be considered as potential candidates for biomarkers useful in diagnosis and differentiation of studied diseases.
Daniel Zalewski; Karol Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Przemysław Kołodziej; Jolanta Szymańska; Bartosz Płachno; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens. International Journal of Molecular Sciences 2021, 22, 3200 .
AMA StyleDaniel Zalewski, Karol Ruszel, Andrzej Stępniewski, Dariusz Gałkowski, Jacek Bogucki, Przemysław Kołodziej, Jolanta Szymańska, Bartosz Płachno, Tomasz Zubilewicz, Marcin Feldo, Janusz Kocki, Anna Bogucka-Kocka. Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens. International Journal of Molecular Sciences. 2021; 22 (6):3200.
Chicago/Turabian StyleDaniel Zalewski; Karol Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Przemysław Kołodziej; Jolanta Szymańska; Bartosz Płachno; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. 2021. "Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens." International Journal of Molecular Sciences 22, no. 6: 3200.
There is an urgent need to seek new molecular biomarkers helpful in diagnosing and treating breast cancer. In this elaboration, we performed a molecular analysis of mutations and expression of genes within the PI3K/Akt/mTOR pathway in patients with ductal breast cancer of various malignancy levels. We recognized significant correlations between the expression levels of the studied genes. We also performed a bioinformatics analysis of the data available on the international database TCGA and compared them with our own research. Studies on mutations and expression of genes were conducted using High-Resolution Melt PCR (HRM-PCR), Allele-Specific-quantitative PCR (ASP-qPCR), Real-Time PCR molecular methods in a group of women with ductal breast cancer. Bioinformatics analysis was carried out using web source Ualcan and bc-GenExMiner. In the studied group of women, it was observed that the prevalence of mutations in the studied PIK3CA and AKT1 genes was 29.63%. It was stated that the average expression level of the PIK3CA, PIK3R1, PTEN genes in the group of breast cancer patients is lower in comparison to the control group, while the average expression level of the AKT1 and mTOR genes in the studied group was higher in comparison to the control group. It was also indicated that in the group of patients with mutations in the area of the PIK3CA and AKT1 genes, the PIK3CA gene expression level is statistically significantly lower than in the group without mutations. According to our knowledge, we demonstrate, for the first time, that there is a very strong positive correlation between the levels of AKT1 and mTOR gene expression in the case of patients with mutations and without mutations.
Przemysław Kołodziej; Marcin Nicoś; Paweł A. Krawczyk; Jacek Bogucki; Agnieszka Karczmarczyk; Daniel Zalewski; Tomasz Kubrak; Elżbieta Kołodziej; Anna Makuch-Kocka; Barbara Madej-Czerwonka; Bartosz J. Płachno; Janusz Kocki; Anna Bogucka-Kocka. The Correlation of Mutations and Expressions of Genes within the PI3K/Akt/mTOR Pathway in Breast Cancer—A Preliminary Study. International Journal of Molecular Sciences 2021, 22, 2061 .
AMA StylePrzemysław Kołodziej, Marcin Nicoś, Paweł A. Krawczyk, Jacek Bogucki, Agnieszka Karczmarczyk, Daniel Zalewski, Tomasz Kubrak, Elżbieta Kołodziej, Anna Makuch-Kocka, Barbara Madej-Czerwonka, Bartosz J. Płachno, Janusz Kocki, Anna Bogucka-Kocka. The Correlation of Mutations and Expressions of Genes within the PI3K/Akt/mTOR Pathway in Breast Cancer—A Preliminary Study. International Journal of Molecular Sciences. 2021; 22 (4):2061.
Chicago/Turabian StylePrzemysław Kołodziej; Marcin Nicoś; Paweł A. Krawczyk; Jacek Bogucki; Agnieszka Karczmarczyk; Daniel Zalewski; Tomasz Kubrak; Elżbieta Kołodziej; Anna Makuch-Kocka; Barbara Madej-Czerwonka; Bartosz J. Płachno; Janusz Kocki; Anna Bogucka-Kocka. 2021. "The Correlation of Mutations and Expressions of Genes within the PI3K/Akt/mTOR Pathway in Breast Cancer—A Preliminary Study." International Journal of Molecular Sciences 22, no. 4: 2061.
The BIRC (baculoviral IAP repeat‐containing; BIRC) family genes encode for Inhibitor of Apoptosis (IAP) proteins. The dysregulation of the expression levels of the genes in question in cancer tissue as compared to normal tissue suggests that the apoptosis process in cancer cells was disturbed, which may be associated with the development and chemoresistance of triple negative breast cancer (TNBC). In our study, we determined the expression level of eight genes from the BIRC family using the Real-Time PCR method in patients with TNBC and compared the obtained results with clinical data. Additionally, using bioinformatics tools (Ualcan and The Breast Cancer Gene-Expression Miner v4.5 (bc-GenExMiner v4.5)), we compared our data with the data in the Cancer Genome Atlas (TCGA) database. We observed diverse expression pattern among the studied genes in breast cancer tissue. Comparing the expression level of the studied genes with the clinical data, we found that in patients diagnosed with breast cancer under the age of 50, the expression levels of all studied genes were higher compared to patients diagnosed after the age of 50. We observed that in patients with invasion of neoplastic cells into lymphatic vessels and fat tissue, the expression levels of BIRC family genes were lower compared to patients in whom these features were not noted. Statistically significant differences in gene expression were also noted in patients classified into three groups depending on the basis of the Scarff-Bloom and Richardson (SBR) Grading System.
Anna Makuch-Kocka; Janusz Kocki; Anna Brzozowska; Jacek Bogucki; Przemysław Kołodziej; Bartosz J. Płachno; Anna Bogucka-Kocka. The BIRC Family Genes Expression in Patients with Triple Negative Breast Cancer. International Journal of Molecular Sciences 2021, 22, 1820 .
AMA StyleAnna Makuch-Kocka, Janusz Kocki, Anna Brzozowska, Jacek Bogucki, Przemysław Kołodziej, Bartosz J. Płachno, Anna Bogucka-Kocka. The BIRC Family Genes Expression in Patients with Triple Negative Breast Cancer. International Journal of Molecular Sciences. 2021; 22 (4):1820.
Chicago/Turabian StyleAnna Makuch-Kocka; Janusz Kocki; Anna Brzozowska; Jacek Bogucki; Przemysław Kołodziej; Bartosz J. Płachno; Anna Bogucka-Kocka. 2021. "The BIRC Family Genes Expression in Patients with Triple Negative Breast Cancer." International Journal of Molecular Sciences 22, no. 4: 1820.
The link between the kynurenine pathway and immunomodulatory molecules—fractalkine and soluble intercellular adhesion molecule-1 (sICAM-1)—in anorexia nervosa (AN) remains unknown. Fractalkine, sICAM-1, tryptophan (TRP), kynurenine (KYN), neuroprotective kynurenic acid (KYNA), neurotoxic 3-OH-kynurenine (3-OH-KYN), and the expression of mRNA for kynurenine aminotransferases (KAT1-3) were studied in 20 female patients with restrictive AN (mostly drug-free, all during first episode of the disease) and in 24 controls. In AN, serum fractalkine, but not sICAM-1, KYNA, KYN, TRP or 3-OH-KYN, was higher; ratios TRP/KYN, KYN/KYNA, KYN/3-OH-KYN and KYNA/3-OH-KYN were unaltered. The expression of the gene encoding KAT3, but not of genes encoding KAT1 and KAT2 (measured in blood mononuclear cells), was higher in patients with AN. In AN, fractalkine positively correlated with TRP, while sICAM-1 was negatively associated with 3-OH-KYN and positively linked with the ratio KYN/3-OH-KYN. Furthermore, TRP and fractalkine were negatively associated with the body mass index (BMI) in AN. Expression of KAT1, KAT2 and KAT3 did not correlate with fractalkine, sICAM-1 or BMI, either in AN or control. Increased fractalkine may be an independent factor associated with the restrictive type of AN. Excessive physical activity probably underlies increased expression of KAT3 observed among enrolled patients. Further, longitudinal studies on a larger cohort of patients should be aimed to clarify the contribution of fractalkine and KAT3 to the pathogenesis of AN.
Ewa Dudzińska; Kinga Szymona; Renata Kloc; Tomasz Kocki; Paulina Gil-Kulik; Jacek Bogucki; Janusz Kocki; Roman Paduch; Ewa Urbańska. Fractalkine, sICAM-1 and Kynurenine Pathway in Restrictive Anorexia Nervosa–Exploratory Study. Nutrients 2021, 13, 339 .
AMA StyleEwa Dudzińska, Kinga Szymona, Renata Kloc, Tomasz Kocki, Paulina Gil-Kulik, Jacek Bogucki, Janusz Kocki, Roman Paduch, Ewa Urbańska. Fractalkine, sICAM-1 and Kynurenine Pathway in Restrictive Anorexia Nervosa–Exploratory Study. Nutrients. 2021; 13 (2):339.
Chicago/Turabian StyleEwa Dudzińska; Kinga Szymona; Renata Kloc; Tomasz Kocki; Paulina Gil-Kulik; Jacek Bogucki; Janusz Kocki; Roman Paduch; Ewa Urbańska. 2021. "Fractalkine, sICAM-1 and Kynurenine Pathway in Restrictive Anorexia Nervosa–Exploratory Study." Nutrients 13, no. 2: 339.
Paweł Aleksandrowicz; Lidia Zofia Kotuła; Katarzyna Grabowska; Rafał Leszek Krakowiak; Marta Kusa-Podkańska; Justyna Agier; Aneta Gorący; Kinga Eleonora Kądziołka; Janusz Kocki; Joanna Wysokińska-Miszczuk. Evaluation of circulating CD34+ stem cells in peripheral venous blood in patients with varying degrees of periodontal disease. Annals of Agricultural and Environmental Medicine 2020, 1 .
AMA StylePaweł Aleksandrowicz, Lidia Zofia Kotuła, Katarzyna Grabowska, Rafał Leszek Krakowiak, Marta Kusa-Podkańska, Justyna Agier, Aneta Gorący, Kinga Eleonora Kądziołka, Janusz Kocki, Joanna Wysokińska-Miszczuk. Evaluation of circulating CD34+ stem cells in peripheral venous blood in patients with varying degrees of periodontal disease. Annals of Agricultural and Environmental Medicine. 2020; ():1.
Chicago/Turabian StylePaweł Aleksandrowicz; Lidia Zofia Kotuła; Katarzyna Grabowska; Rafał Leszek Krakowiak; Marta Kusa-Podkańska; Justyna Agier; Aneta Gorący; Kinga Eleonora Kądziołka; Janusz Kocki; Joanna Wysokińska-Miszczuk. 2020. "Evaluation of circulating CD34+ stem cells in peripheral venous blood in patients with varying degrees of periodontal disease." Annals of Agricultural and Environmental Medicine , no. : 1.
Abdominal artery aneurysm (AAA) refers to abdominal aortic dilatation of 3 cm or greater. AAA is frequently underdiagnosed due to often asymptomatic character of the disease, leading to elevated mortality due to aneurysm rupture. MiRNA constitute a pool of small RNAs controlling gene expression and is involved in many pathologic conditions in human. Targeted panel detecting altered expression of miRNA and genes involved in AAA would improve early diagnosis of this disease. In the presented study, we selected and analyzed miRNA and gene expression signatures in AAA patients. Next, generation sequencing was applied to obtain miRNA and gene-wide expression profiles from peripheral blood mononuclear cells in individuals with AAA and healthy controls. Differential expression analysis was performed using DESeq2 and uninformative variable elimination by partial least squares (UVE-PLS) methods. A total of 31 miRNAs and 51 genes were selected as the most promising biomarkers of AAA. Receiver operating characteristics (ROC) analysis showed good diagnostic ability of proposed biomarkers. Genes regulated by selected miRNAs were determined in silico and associated with functional terms closely related to cardiovascular and neurological diseases. Proposed biomarkers may be used for new diagnostic and therapeutic approaches in management of AAA. The findings will also contribute to the pool of knowledge about miRNA-dependent regulatory mechanisms involved in pathology of that disease.
Daniel Zalewski; Karol Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Łukasz Komsta; Przemysław Kołodziej; Paulina Chmiel; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. Dysregulation of microRNA Modulatory Network in Abdominal Aortic Aneurysm. Journal of Clinical Medicine 2020, 9, 1974 .
AMA StyleDaniel Zalewski, Karol Ruszel, Andrzej Stępniewski, Dariusz Gałkowski, Jacek Bogucki, Łukasz Komsta, Przemysław Kołodziej, Paulina Chmiel, Tomasz Zubilewicz, Marcin Feldo, Janusz Kocki, Anna Bogucka-Kocka. Dysregulation of microRNA Modulatory Network in Abdominal Aortic Aneurysm. Journal of Clinical Medicine. 2020; 9 (6):1974.
Chicago/Turabian StyleDaniel Zalewski; Karol Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Łukasz Komsta; Przemysław Kołodziej; Paulina Chmiel; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. 2020. "Dysregulation of microRNA Modulatory Network in Abdominal Aortic Aneurysm." Journal of Clinical Medicine 9, no. 6: 1974.
Anna Beata Pacian; Janusz Kocki; Jolanta Pacian; Monika Kaczoruk; Maria Bylina; Paulina Kaczor-Szkodny; Elżbieta Monika Galińska; Teresa Kulik; Lech Panasiuk. An evaluation of the genetic conditioning of evoking pain. Annals of Agricultural and Environmental Medicine 2020, 27, 274 -278.
AMA StyleAnna Beata Pacian, Janusz Kocki, Jolanta Pacian, Monika Kaczoruk, Maria Bylina, Paulina Kaczor-Szkodny, Elżbieta Monika Galińska, Teresa Kulik, Lech Panasiuk. An evaluation of the genetic conditioning of evoking pain. Annals of Agricultural and Environmental Medicine. 2020; 27 (2):274-278.
Chicago/Turabian StyleAnna Beata Pacian; Janusz Kocki; Jolanta Pacian; Monika Kaczoruk; Maria Bylina; Paulina Kaczor-Szkodny; Elżbieta Monika Galińska; Teresa Kulik; Lech Panasiuk. 2020. "An evaluation of the genetic conditioning of evoking pain." Annals of Agricultural and Environmental Medicine 27, no. 2: 274-278.
Chronic venous disease (CVD) is a vascular disease of lower limbs with high prevalence worldwide. Pathologic features include varicose veins, venous valves dysfunction and skin ulceration resulting from dysfunction of cell proliferation, apoptosis and angiogenesis. These processes are partly regulated by microRNA (miRNA)-dependent modulation of gene expression, pointing to miRNA as a potentially important target in diagnosis and therapy of CVD progression. The aim of the study was to analyze alterations of miRNA and gene expression in CVD, as well as to identify miRNA-mediated changes in gene expression and their potential link to CVD development. Using next generation sequencing, miRNA and gene expression profiles in peripheral blood mononuclear cells of subjects with CVD in relation to healthy controls were studied. Thirty-one miRNAs and 62 genes were recognized as potential biomarkers of CVD using DESeq2, Uninformative Variable Elimination by Partial Least Squares (UVE-PLS) and ROC (Receiver Operating Characteristics) methods. Regulatory interactions between potential biomarker miRNAs and genes were projected. Functional analysis of microRNA-regulated genes revealed terms closely related to cardiovascular diseases and risk factors. The study shed new light on miRNA-dependent regulatory mechanisms involved in the pathology of CVD. MicroRNAs and genes proposed as CVD biomarkers may be used to develop new diagnostic and therapeutic methods.
Daniel P. Zalewski; Karol P. Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Łukasz Komsta; Przemysław Kołodziej; Paulina Chmiel; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. Dysregulations of MicroRNA and Gene Expression in Chronic Venous Disease. Journal of Clinical Medicine 2020, 9, 1251 .
AMA StyleDaniel P. Zalewski, Karol P. Ruszel, Andrzej Stępniewski, Dariusz Gałkowski, Jacek Bogucki, Łukasz Komsta, Przemysław Kołodziej, Paulina Chmiel, Tomasz Zubilewicz, Marcin Feldo, Janusz Kocki, Anna Bogucka-Kocka. Dysregulations of MicroRNA and Gene Expression in Chronic Venous Disease. Journal of Clinical Medicine. 2020; 9 (5):1251.
Chicago/Turabian StyleDaniel P. Zalewski; Karol P. Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Łukasz Komsta; Przemysław Kołodziej; Paulina Chmiel; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. 2020. "Dysregulations of MicroRNA and Gene Expression in Chronic Venous Disease." Journal of Clinical Medicine 9, no. 5: 1251.
Background Acute myeloid leukemia (AML) is a heterogenic lethal disorder characterized by the accumulation of abnormal myeloid progenitor cells in the bone marrow which results in hematopoietic failure. Despite various efforts in detection and treatment, many patients with AML die of this cancer. That is why it is important to develop novel therapeutic options, employing strategic target genes involved in apoptosis and tumor progression. Methods The aim of the study was to evaluate PARP1, PARP2, PARP3, and TRPM2 gene expression at mRNA level using qPCR method in the cells of hematopoietic system of the bone marrow in patients with acute myeloid leukemia, bone marrow collected from healthy patients, peripheral blood of healthy individuals, and hematopoietic stem cells from the peripheral blood after mobilization. Results The results found that the bone marrow cells of the patients with acute myeloid leukemia (AML) show overexpression of PARP1 and PARP2 genes and decreased TRPM2 gene expression. In the hematopoietic stem cells derived from the normal marrow and peripheral blood after mobilization, the opposite situation was observed, i.e. TRPM2 gene showed increased expression while PARP1 and PARP2 gene expression was reduced. We observed positive correlations between PARP1, PARP2, PARP3, and TRPM2 genes expression in the group of mature mononuclear cells derived from the peripheral blood and in the group of bone marrow-derived cells. In AML cells significant correlations were not observed between the expression of the examined genes. In addition, we observed that the reduced expression of TRPM2 and overexpression of PARP1 are associated with a shorter overall survival of patients, indicating the prognostic significance of these genes expression in AML. Conclusions Our research suggests that in physiological conditions in the cells of the hematopoietic system there is mutual positive regulation of PARP1, PARP2, PARP3, and TRPM2 genes expression. PARP1, PARP2, and TRPM2 genes at mRNA level deregulate in acute myeloid leukemia cells.
Paulina Gil-Kulik; Ewa Dudzińska; Elżbieta Radzikowska-Büchner; Joanna Wawer; Mariusz Jojczuk; Adam Nogalski; Genowefa Anna Wawer; Marcin Feldo; Wojciech Kocki; Maria Cioch; Anna Bogucka-Kocka; Mansur Rahnama; Janusz Kocki. Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells. 2020, 1 .
AMA StylePaulina Gil-Kulik, Ewa Dudzińska, Elżbieta Radzikowska-Büchner, Joanna Wawer, Mariusz Jojczuk, Adam Nogalski, Genowefa Anna Wawer, Marcin Feldo, Wojciech Kocki, Maria Cioch, Anna Bogucka-Kocka, Mansur Rahnama, Janusz Kocki. Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells. . 2020; ():1.
Chicago/Turabian StylePaulina Gil-Kulik; Ewa Dudzińska; Elżbieta Radzikowska-Büchner; Joanna Wawer; Mariusz Jojczuk; Adam Nogalski; Genowefa Anna Wawer; Marcin Feldo; Wojciech Kocki; Maria Cioch; Anna Bogucka-Kocka; Mansur Rahnama; Janusz Kocki. 2020. "Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells." , no. : 1.
The aim of the study was the analysis of (1) the level of BAX,BCL2,BIRC6,CASP3, CASP9 apoptosis genes expression in schizophrenic patients and in the control group, and (2) the relationships between the genes expression level and the morphological and biochemical parameters, as well as the severity of psychopathological symptoms. The study included 21 patients diagnosed with schizophrenia according to ICD-10 and 26 healthy subjects. The following parameters were assessed: genes expression in peripheral blood lymphocytes, laboratory parameters and severity of psychopathological symptoms (using the PANSS). The expression of the BCL2 gene and the CASP3 gene was significantly higher in schizophrenic patients compared to the controls. A significant positive correlation was found between the BAX gene expression level and neutrophil, lymphocyte and monocyte counts as well as the severity of negative symptoms (PANSS-N), between BCL2 and CASP9 genes expression and the monocyte count, and between the CASP3 gene expression and the lymphocyte count. (1) Significantly higher expression of BCL2 and CASP3 genes in schizophrenic patients compared to the controls proves the intensification of the apoptosis process, fitting in the theory of increased apoptosis in the pathophysiology of schizophrenia. (2) Significant correlations between the BAX gene expression and differential blood count parameters (leucocyte, neutrophil, lymphocyte, monocyte count) in the group of schizophrenic patients suggest a relationship with immune dysregulation and confirm the presence of apoptosis in peripheral blood lymphocytes. (3) The BAX gene expression may be indicative of the severity of negative symptoms in schizophrenia. (4) The analysis of the intercorrelation of studied genes expression indicates that BAX and CASP3 genes were the most active in the apoptosis process in the study group.
Kinga Szymona; Ewa Dudzińska; Hanna Karakuła-Juchnowicz; Paulina Gil-Kulik; Piotr Chomik; Małgorzata Świstowska; Joanna Gałaszkiewicz; Janusz Kocki. Analysis of the expression of BAX, BCL2, BIRC6, CASP3, CASP9 apoptosis genes during the first episode of schizophrenia. Psychiatria Polska 2019, 53, 1293 -1303.
AMA StyleKinga Szymona, Ewa Dudzińska, Hanna Karakuła-Juchnowicz, Paulina Gil-Kulik, Piotr Chomik, Małgorzata Świstowska, Joanna Gałaszkiewicz, Janusz Kocki. Analysis of the expression of BAX, BCL2, BIRC6, CASP3, CASP9 apoptosis genes during the first episode of schizophrenia. Psychiatria Polska. 2019; 53 (6):1293-1303.
Chicago/Turabian StyleKinga Szymona; Ewa Dudzińska; Hanna Karakuła-Juchnowicz; Paulina Gil-Kulik; Piotr Chomik; Małgorzata Świstowska; Joanna Gałaszkiewicz; Janusz Kocki. 2019. "Analysis of the expression of BAX, BCL2, BIRC6, CASP3, CASP9 apoptosis genes during the first episode of schizophrenia." Psychiatria Polska 53, no. 6: 1293-1303.
Acute myeloid leukemia (AML) is a heterogenic lethal disorder characterized by the accumulation of abnormal myeloid progenitor cells in the bone marrow, which results in hematopoietic failure. Despite various efforts in detection and treatment, many patients with AML die of this cancer. That is why it is important to develop novel therapeutic options, employing strategic target genes involved in apoptosis and tumor progression. The aim of the study was to evaluate PARP1, PARP2, PARP3, and TRPM2 gene expression at the mRNA level in the cells of the hematopoietic system of the bone marrow in patients with acute myeloid leukemia, bone marrow collected from healthy patients, peripheral blood of healthy individuals, and hematopoietic stem cells from the peripheral blood after mobilization.Results: The results found that the bone marrow cells of patients with acute myeloid leukemia (AML) show over expression of PARP1 and PARP2 genes and decreased TRPM2 gene expression. In the hematopoietic stem cells derived from the normal marrow and peripheral blood after mobilization, the opposite situation was observed, i.e. TRPM2 gene showed increased expression while PARP1 and PARP2 gene expression was reduced. We observed the positive correlations between PARP1, PARP2, PARP3, and TRPM2 genes expression in the group of mature mononuclear cells derived from the peripheral blood and in the group of bone marrow-derived cells. In AML cells significant correlations were not observed between the expression of the examined genes.Conclusions: Our research suggests that in physiological conditions in the cells of the hematopoietic system there is mutual positive regulation of PARP1, PARP2, PARP3, and TRPM2 genes expression. PARP1, PARP2, and TRPM2 genes at mRNA level deregulate in acute myeloid leukemia cells.
Paulina Gil-Kulik; Ewa Dudzińska; Elżbieta Radzikowska-Büchner; Joanna Wawer; Mariusz Jojczuk; Adam Nogalski; Genowefa Anna Wawer; Marcin Feldo; Wojciech Kocki; Maria Cioch; Anna Bogucka-Kocka; Mansur Rahnama; Janusz Kocki. Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells. 2019, 1 .
AMA StylePaulina Gil-Kulik, Ewa Dudzińska, Elżbieta Radzikowska-Büchner, Joanna Wawer, Mariusz Jojczuk, Adam Nogalski, Genowefa Anna Wawer, Marcin Feldo, Wojciech Kocki, Maria Cioch, Anna Bogucka-Kocka, Mansur Rahnama, Janusz Kocki. Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells. . 2019; ():1.
Chicago/Turabian StylePaulina Gil-Kulik; Ewa Dudzińska; Elżbieta Radzikowska-Büchner; Joanna Wawer; Mariusz Jojczuk; Adam Nogalski; Genowefa Anna Wawer; Marcin Feldo; Wojciech Kocki; Maria Cioch; Anna Bogucka-Kocka; Mansur Rahnama; Janusz Kocki. 2019. "Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells." , no. : 1.
Diosmin is a natural compound with a wide range of biological activity, e.g., it improves lymphatic drainage, supports microcirculation, and increases venous tone, and venous elasticity, hence, it is applied in the pharmacotherapy of chronic venous disorders (CVD). The aim of this study was to assess the correlation between diosmin administration (2 × 600 mg daily) in patients suffering from CVD and the levels of selected factors influencing angiogenesis, which are involved in CVD pathophysiology. Thirty-five CVD patients were examined. Levels of plasma tumor necrosis factor alpha (TNF alpha), vascular endothelial growth factor (VEGF-A and VEGF-C); angiostatin, interleukin 6 (IL-6), fibroblast growth factor 2 (FGF2); and plasminogen (PLG) were measured with an Elisa assay before and after three months of diosmin administration. The clinical symptoms of CVD were monitored using ultrasound images, echo Doppler assay, visual analogue scale (VAS), and measurement of the leg circumference. The average content of TNF alpha, VEGF-C, VEGF-A IL-6, and FGF2 decreased after the therapy with diosmin in a significant manner; with p < 0.001, p < 0.05, p < 0.05, p < 0.01, and p < 0.01, respectively, and a significant (p < 0.05) increase in the plasma angiostatin level after the three-month treatment was found. A significant (p < 0.05) decrease in edema and the average leg circumference of the patients was observed after the therapy. Diosmin influences the angiogenic and inflammatory mechanisms involved in the pathophysiology of edema presented in patients with a different class of CVD.
Marcin Feldo; Magdalena Wójciak-Kosior; Ireneusz Sowa; Janusz Kocki; Jacek Bogucki; Tomasz Zubilewicz; Jan Kęsik; Anna Bogucka-Kocka. Effect of Diosmin Administration in Patients with Chronic Venous Disorders on Selected Factors Affecting Angiogenesis. Molecules 2019, 24, 3316 .
AMA StyleMarcin Feldo, Magdalena Wójciak-Kosior, Ireneusz Sowa, Janusz Kocki, Jacek Bogucki, Tomasz Zubilewicz, Jan Kęsik, Anna Bogucka-Kocka. Effect of Diosmin Administration in Patients with Chronic Venous Disorders on Selected Factors Affecting Angiogenesis. Molecules. 2019; 24 (18):3316.
Chicago/Turabian StyleMarcin Feldo; Magdalena Wójciak-Kosior; Ireneusz Sowa; Janusz Kocki; Jacek Bogucki; Tomasz Zubilewicz; Jan Kęsik; Anna Bogucka-Kocka. 2019. "Effect of Diosmin Administration in Patients with Chronic Venous Disorders on Selected Factors Affecting Angiogenesis." Molecules 24, no. 18: 3316.
Isoquinoline alkaloids belong to the toxic secondary metabolites occurring in plants of many families. The high biological activity makes these compounds promising agents for use in medicine, particularly as anticancer drugs. The aim of our study was to evaluate the cytotoxicity and proapoptotic activity of sanguinarine, berberine, and extracts of Chelidonium majus L. and Berberis thunbergii DC. IC10, IC50, and IC90 doses were established toward hematopoietic cancer cell lines using trypan blue staining. Alterations in the expression of 18 apoptosis-related genes in cells exposed to IC10, IC50, and IC90 were evaluated using real-time PCR. Sanguinarine and Chelidonium majus L. extract exhibit significant cytotoxicity against all studied cell lines. Lower cytotoxic activity was demonstrated for berberine. Berberis thunbergii DC. extract had no influence on cell viability. Berberine, sanguinarine, and Chelidonium majus L. extract altered the expression of apoptosis-related genes in all tested cell lines, indicating the induction of apoptosis. The presented study confirmed the substantial cytotoxicity and proapoptotic activity of sanguinarine, berberine, and Chelidonium majus L. extract toward the studied hematopoietic cell lines, which indicates the utility of these substances in anticancer therapy.
Anna Och; Daniel Zalewski; Łukasz Komsta; Przemysław Kołodziej; Janusz Kocki; Anna Bogucka-Kocka. Cytotoxic and Proapoptotic Activity of Sanguinarine, Berberine, and Extracts of Chelidonium majus L. and Berberis thunbergii DC. toward Hematopoietic Cancer Cell Lines. Toxins 2019, 11, 485 .
AMA StyleAnna Och, Daniel Zalewski, Łukasz Komsta, Przemysław Kołodziej, Janusz Kocki, Anna Bogucka-Kocka. Cytotoxic and Proapoptotic Activity of Sanguinarine, Berberine, and Extracts of Chelidonium majus L. and Berberis thunbergii DC. toward Hematopoietic Cancer Cell Lines. Toxins. 2019; 11 (9):485.
Chicago/Turabian StyleAnna Och; Daniel Zalewski; Łukasz Komsta; Przemysław Kołodziej; Janusz Kocki; Anna Bogucka-Kocka. 2019. "Cytotoxic and Proapoptotic Activity of Sanguinarine, Berberine, and Extracts of Chelidonium majus L. and Berberis thunbergii DC. toward Hematopoietic Cancer Cell Lines." Toxins 11, no. 9: 485.
Background and objectives: Inflammatory bowel disease (IBD) mainly includes Crohn’s disease (CD) and ulcerative colitis (UC). Both conditions are associated with an exacerbated intestinal immune response to harmless stimuli, leading to upregulation of pro-inflammatory mediators. Materials and Methods: The subjects of the study were 55 patients with IBD. The control group consisted of 35 healthy subjects. The researched material consisted of peripheral blood lymphocytes collected from the subjects. Expression of the genes BAX, BCL2, CASP3 and CASP9 was assessed at the mRNA level in the peripheral blood lymphocytes of patients with ulcerative colitis and Crohn’s disease relative to the healthy subjects. The expression of the genes was determined by rtPCR using TaqMan probes specific for these genes. Results: The group of patients diagnosed with CD had statistically significantly higher expression of the genes BAX (p = 0.012), BCL2 (p = 0.022), CASP3 (p = 0.003) and CASP9 (p = 0.029) than healthy subjects. Expression of BAX, BCL2, CASP3 and CASP9 in UC patients in the active phase of the disease was significantly lower than in patients in remission: BAX (p = 0.001), BCL2 (p = 0.038) and CASP9 (p = 0.007). In patients with UC, the BAX/BCL2 ratio was significantly correlated (r = 0.473) with the duration of the disease. In the group of CD patients treated biologically, a significantly lower BAX/BCL2 ratio was demonstrated than in patients that were not biologically treated. Conclusions: Our research has shown a simultaneous increase in the expression of the anti-apoptotic BCL2 gene and the proapoptotic BAX gene, which suggests the dysregulation of apoptosis mechanisms in IBD. Significantly higher expression of BAX and BCL2 in UC patients in remission as compared to CD may suggest differences in these diseases in terms of prognosis and treatment. Our results may suggest that an underlying imbalance in factors controlling apoptosis in peripheral blood lymphocytes may be the response of the immune system to inflammation of the intestinal mucosa. Modulation of apoptosis may become an important therapeutic mechanism in IBD.
Ewa Dudzińska; Kinga Szymona; Paulina Gil-Kulik; Piotr Chomik; Małgorzata Świstowska; Magdalena Gryzińska; Janusz Kocki. Imbalance of Controlled Death in Peripheral Blood Lymphocytes in Crohn’s Disease and Ulcerative Colitis. Medicina 2019, 55, 231 .
AMA StyleEwa Dudzińska, Kinga Szymona, Paulina Gil-Kulik, Piotr Chomik, Małgorzata Świstowska, Magdalena Gryzińska, Janusz Kocki. Imbalance of Controlled Death in Peripheral Blood Lymphocytes in Crohn’s Disease and Ulcerative Colitis. Medicina. 2019; 55 (6):231.
Chicago/Turabian StyleEwa Dudzińska; Kinga Szymona; Paulina Gil-Kulik; Piotr Chomik; Małgorzata Świstowska; Magdalena Gryzińska; Janusz Kocki. 2019. "Imbalance of Controlled Death in Peripheral Blood Lymphocytes in Crohn’s Disease and Ulcerative Colitis." Medicina 55, no. 6: 231.
Brain ischemia may be causally related with Alzheimer's disease. Presumably, β-secretase and amyloid-β protein precursor gene expression changes may be associated with Alzheimer's disease neuropathology. Consequently, we have examined quantitative changes in both β-secretase and amyloid-β protein precursor genes in the medial temporal lobe cortex with the use of quantitative rtPCR analysis following 10-min global brain ischemia in rats with survival of 2, 7, and 30 days. The greatest significant overexpression of β-secretase gene was noted on the 2nd day, while on days 7-30 the expression of this gene was only modestly downregulated. Amyloid-β protein precursor gene was downregulated on the 2nd day, but on days 7-30 postischemia, there was a significant reverse tendency. Thus, the demonstrated alterations indicate that the considerable changes of expression of β-secretase and amyloid-β protein precursor genes may be connected with a response of neurons in medial temporal lobe cortex to transient global brain ischemia. Finally, the ischemia-induced gene changes may play a key role in a late and slow onset of Alzheimer-type pathology.
Ryszard Pluta; Janusz Kocki; Marzena Ułamek-Kozioł; Alicja Petniak; Paulina Gil-Kulik; Sławomir Januszewski; Jacek Bogucki; Mirosław Jabłoński; Judyta Brzozowska; Wanda Furmaga-Jabłońska; Anna Bogucka-Kocka; Stanisław J. Czuczwar. Discrepancy in Expression of β-Secretase and Amyloid-β Protein Precursor in Alzheimer-Related Genes in the Rat Medial Temporal Lobe Cortex Following Transient Global Brain Ischemia. Journal of Alzheimer's Disease 2016, 51, 1023 -1031.
AMA StyleRyszard Pluta, Janusz Kocki, Marzena Ułamek-Kozioł, Alicja Petniak, Paulina Gil-Kulik, Sławomir Januszewski, Jacek Bogucki, Mirosław Jabłoński, Judyta Brzozowska, Wanda Furmaga-Jabłońska, Anna Bogucka-Kocka, Stanisław J. Czuczwar. Discrepancy in Expression of β-Secretase and Amyloid-β Protein Precursor in Alzheimer-Related Genes in the Rat Medial Temporal Lobe Cortex Following Transient Global Brain Ischemia. Journal of Alzheimer's Disease. 2016; 51 (4):1023-1031.
Chicago/Turabian StyleRyszard Pluta; Janusz Kocki; Marzena Ułamek-Kozioł; Alicja Petniak; Paulina Gil-Kulik; Sławomir Januszewski; Jacek Bogucki; Mirosław Jabłoński; Judyta Brzozowska; Wanda Furmaga-Jabłońska; Anna Bogucka-Kocka; Stanisław J. Czuczwar. 2016. "Discrepancy in Expression of β-Secretase and Amyloid-β Protein Precursor in Alzheimer-Related Genes in the Rat Medial Temporal Lobe Cortex Following Transient Global Brain Ischemia." Journal of Alzheimer's Disease 51, no. 4: 1023-1031.
Brain ischemia may be causally related with Alzheimer's disease. Probably, presenilin gene dysregulation may be associated with Alzheimer's disease neuropathology. Consequently, we have examined quantitative changes in both presenilin 1 and 2 genes in the medial temporal lobe cortex following 10-min global brain ischemia in rats. Global brain ischemia was induced by cardiac arrest in female rats that were allowed to survive for 2, 7 and 30 days. The expression of presenilin genes was evaluated in the rat medial temporal lobe cortex with the use of quantitative RT-PCR analysis. Presenilin 1 gene expression tended to be downregulated from days 2 to 7 postischemia but at day 30, there was a reverse tendency. The greatest overexpression of presenilin 2 gene was noted at 2-nd day whilst on day 7, the expression of this gene was only modestly elevated. Eventually, at day 30 expression of presenilin 2 gene was modestly downregulated. Alterations of presenilin 2 gene expression between 2 and 7 days and between 2 and 30 days were statistically significant. Thus, presented changes suggest that the significant dysregulation of presenilin 2 gene may be connected with a response of neuronal cells to transient global brain ischemia due to cardiac arrest. Finally, the ischemia-induced gene dysregulation may play a key role in the late onset of Alzheimer's-type dementia.
Ryszard Pluta; Janusz Kocki; Marzena Ułamek-Kozioł; Anna Bogucka-Kocka; Paulina Gil-Kulik; Sławomir Januszewski; Mirosław Jabłoński; Alicja Petniak; Judyta Brzozowska; Jacek Bogucki; Wanda Furmaga-Jabłońska; Stanisław J. Czuczwar. Alzheimer-associated presenilin 2 gene is dysregulated in rat medial temporal lobe cortex after complete brain ischemia due to cardiac arrest. Pharmacological Reports 2016, 68, 155 -161.
AMA StyleRyszard Pluta, Janusz Kocki, Marzena Ułamek-Kozioł, Anna Bogucka-Kocka, Paulina Gil-Kulik, Sławomir Januszewski, Mirosław Jabłoński, Alicja Petniak, Judyta Brzozowska, Jacek Bogucki, Wanda Furmaga-Jabłońska, Stanisław J. Czuczwar. Alzheimer-associated presenilin 2 gene is dysregulated in rat medial temporal lobe cortex after complete brain ischemia due to cardiac arrest. Pharmacological Reports. 2016; 68 (1):155-161.
Chicago/Turabian StyleRyszard Pluta; Janusz Kocki; Marzena Ułamek-Kozioł; Anna Bogucka-Kocka; Paulina Gil-Kulik; Sławomir Januszewski; Mirosław Jabłoński; Alicja Petniak; Judyta Brzozowska; Jacek Bogucki; Wanda Furmaga-Jabłońska; Stanisław J. Czuczwar. 2016. "Alzheimer-associated presenilin 2 gene is dysregulated in rat medial temporal lobe cortex after complete brain ischemia due to cardiac arrest." Pharmacological Reports 68, no. 1: 155-161.
The stromal-vascular cell fraction (SVF) of adipose tissue can be an abundant source of both multipotent and pluripotent stem cells, known as adipose-derived stem cells or adipose tissue-derived stromal cells (ADSCs). The SVF also contains vascular cells, targeted progenitor cells, and preadipocytes. Stromal cells isolated from adipose tissue express common surface antigens, show the ability to adhere to plastic, and produce forms that resemble fibroblasts. They are characterized by a high proliferation potential and the ability to differentiate into cells of meso-, ecto- and endodermal origin. Although stem cells obtained from an adult organism have smaller capabilities for differentiation in comparison to embryonic and induced pluripotent stem cells (iPSs), the cost of obtaining them is significantly lower. The 40 years of research that mainly focused on the potential of bone marrow stem cells (BMSCs) revealed a number of negative factors: the painful sampling procedure, frequent complications, and small cell yield. The number of stem cells in adipose tissue is relatively large, and obtaining them is less invasive. Sampling through simple procedures such as liposuction performed under local anesthesia is less painful, ensuring patient comfort. The isolated cells are easily grown in culture, and they retain their properties over many passages. That is why adipose tissue has recently been treated as an attractive alternative source of stem cells. Essential aspects of ADSC biology and their use in regenerative medicine will be analyzed in this article.
Izabela Harasymiak-Krzyżanowska; Alicja Niedojadło; Jolanta Karwat; Lidia Kotuła; Paulina Gil-Kulik; Magdalena Sawiuk; Janusz Kocki. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cellular & Molecular Biology Letters 2013, 18, 479 -493.
AMA StyleIzabela Harasymiak-Krzyżanowska, Alicja Niedojadło, Jolanta Karwat, Lidia Kotuła, Paulina Gil-Kulik, Magdalena Sawiuk, Janusz Kocki. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cellular & Molecular Biology Letters. 2013; 18 (4):479-493.
Chicago/Turabian StyleIzabela Harasymiak-Krzyżanowska; Alicja Niedojadło; Jolanta Karwat; Lidia Kotuła; Paulina Gil-Kulik; Magdalena Sawiuk; Janusz Kocki. 2013. "Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications." Cellular & Molecular Biology Letters 18, no. 4: 479-493.