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The innovative Eye Movement Modelling Examples (EMMEs) method can be used in medicine as an educational training tool for the assessment and verification of students and professionals. Our work was intended to analyse the possibility of using eye tracking tools to verify the skills and training of people engaged in laboratory medicine on the example of parasitological diagnostics. Professionally active laboratory diagnosticians working in a multi-profile laboratory (non-parasitological) (n = 16), laboratory diagnosticians no longer working in this profession (n = 10), and medical analyst students (n = 56), participated in the study. The studied group analysed microscopic images of parasitological preparations made with the cellSens Dimension Software (Olympus) system. Eye activity parameters were obtained using a stationary, video-based eye tracker Tobii TX300 which has a 3-ms temporal resolution. Eye movement activity parameters were analysed along with time parameters. The results of our studies have shown that the eye tracking method is a valuable tool for the analysis of parasitological preparations. Detailed quantitative and qualitative analysis confirmed that the EMMEs method may facilitate learning of the correct microscopic image scanning path. The analysis of the results of our studies allows us to conclude that the EMMEs method may be a valuable tool in the preparation of teaching materials in virtual microscopy. These teaching materials generated with the use of eye tracking, prepared by experienced professionals in the field of laboratory medicine, can be used during various training, simulations and courses in medical parasitology and contribute to the verification of education results, professional skills, and elimination of errors in parasitological diagnostics.
Przemysław Kołodziej; Wioletta Tuszyńska-Bogucka; Mariusz Dzieńkowski; Jacek Bogucki; Janusz Kocki; Marek Milosz; Marcin Kocki; Patrycja Reszka; Wojciech Kocki; Anna Bogucka-Kocka. Eye Tracking—An Innovative Tool in Medical Parasitology. Journal of Clinical Medicine 2021, 10, 2989 .
AMA StylePrzemysław Kołodziej, Wioletta Tuszyńska-Bogucka, Mariusz Dzieńkowski, Jacek Bogucki, Janusz Kocki, Marek Milosz, Marcin Kocki, Patrycja Reszka, Wojciech Kocki, Anna Bogucka-Kocka. Eye Tracking—An Innovative Tool in Medical Parasitology. Journal of Clinical Medicine. 2021; 10 (13):2989.
Chicago/Turabian StylePrzemysław Kołodziej; Wioletta Tuszyńska-Bogucka; Mariusz Dzieńkowski; Jacek Bogucki; Janusz Kocki; Marek Milosz; Marcin Kocki; Patrycja Reszka; Wojciech Kocki; Anna Bogucka-Kocka. 2021. "Eye Tracking—An Innovative Tool in Medical Parasitology." Journal of Clinical Medicine 10, no. 13: 2989.
In most angiosperms, the female gametophyte is hidden in the mother tissues and the pollen tube enters the ovule via a micropylar canal. The mother tissues play an essential role in the pollen tube guidance. However, in Utricularia, the female gametophyte surpasses the entire micropylar canal and extends beyond the limit of the integument. The female gametophyte then invades the placenta and a part of the central cell has direct contact with the ovary chamber. To date, information about the role of the placenta and integument in pollen tube guidance in Utricularia, which have extra-ovular female gametophytes, has been lacking. The aim of this study was to evaluate the role of the placenta, central cell and integument in pollen tube pollen tube guidance in Utricularia nelumbifolia Gardner and Utricularia humboldtii R.H. Schomb. by studying the production of arabinogalactan proteins. It was also determined whether the production of the arabinogalactan proteins is dependent on pollination in Utricularia. In both of the examined species, arabinogalactan proteins (AGPs) were observed in the placenta (epidermis and nutritive tissue), ovule (integument, chalaza), and female gametophyte of both pollinated and unpollinated flowers, which means that the production of AGPs is independent of pollination; however, the production of some AGPs was lower after fertilization. There were some differences in the production of AGPs between the examined species. The occurrence of AGPs in the placental epidermis and nutritive tissue suggests that they function as an obturator. The production of some AGPs in the ovular tissues (nucellus, integument) was independent of the presence of a mature embryo sac.
Bartosz Płachno; Małgorzata Kapusta; Piotr Świątek; Krzysztof Banaś; Vitor Miranda; Anna Bogucka-Kocka. Spatio-Temporal Distribution of Cell Wall Components in the Placentas, Ovules and Female Gametophytes of Utricularia during Pollination. International Journal of Molecular Sciences 2021, 22, 5622 .
AMA StyleBartosz Płachno, Małgorzata Kapusta, Piotr Świątek, Krzysztof Banaś, Vitor Miranda, Anna Bogucka-Kocka. Spatio-Temporal Distribution of Cell Wall Components in the Placentas, Ovules and Female Gametophytes of Utricularia during Pollination. International Journal of Molecular Sciences. 2021; 22 (11):5622.
Chicago/Turabian StyleBartosz Płachno; Małgorzata Kapusta; Piotr Świątek; Krzysztof Banaś; Vitor Miranda; Anna Bogucka-Kocka. 2021. "Spatio-Temporal Distribution of Cell Wall Components in the Placentas, Ovules and Female Gametophytes of Utricularia during Pollination." International Journal of Molecular Sciences 22, no. 11: 5622.
Several human tissues are investigated in studies of molecular biomarkers associated with diseases development. Special attention is focused on the blood and its components due to combining abundant information about systemic responses to pathological processes as well as high accessibility. In the current study, transcriptome profiles of peripheral blood mononuclear cells (PBMCs) were used to compare differentially expressed genes between patients with lower extremities arterial disease (LEAD), abdominal aortic aneurysm (AAA) and chronic venous disease (CVD). Gene expression patterns were generated using the Ion S5XL next-generation sequencing platform and were analyzed using DESeq2 and UVE-PLS methods implemented in R programming software. In direct pairwise analysis, 21, 58 and 10 differentially expressed genes were selected from the comparison of LEAD vs. AAA, LEAD vs. CVD and AAA vs. CVD patient groups, respectively. Relationships between expression of dysregulated genes and age, body mass index, creatinine levels, hypertension and medication were identified using Spearman rank correlation test and two-sided Mann–Whitney U test. The functional analysis, performed using DAVID website tool, provides potential implications of selected genes in pathological processes underlying diseases studied. Presented research provides new insight into differences of pathogenesis in LEAD, AAA and CVD, and selected genes could be considered as potential candidates for biomarkers useful in diagnosis and differentiation of studied diseases.
Daniel Zalewski; Karol Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Przemysław Kołodziej; Jolanta Szymańska; Bartosz Płachno; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens. International Journal of Molecular Sciences 2021, 22, 3200 .
AMA StyleDaniel Zalewski, Karol Ruszel, Andrzej Stępniewski, Dariusz Gałkowski, Jacek Bogucki, Przemysław Kołodziej, Jolanta Szymańska, Bartosz Płachno, Tomasz Zubilewicz, Marcin Feldo, Janusz Kocki, Anna Bogucka-Kocka. Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens. International Journal of Molecular Sciences. 2021; 22 (6):3200.
Chicago/Turabian StyleDaniel Zalewski; Karol Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Przemysław Kołodziej; Jolanta Szymańska; Bartosz Płachno; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. 2021. "Identification of Transcriptomic Differences between Lower Extremities Arterial Disease, Abdominal Aortic Aneurysm and Chronic Venous Disease in Peripheral Blood Mononuclear Cells Specimens." International Journal of Molecular Sciences 22, no. 6: 3200.
There is an urgent need to seek new molecular biomarkers helpful in diagnosing and treating breast cancer. In this elaboration, we performed a molecular analysis of mutations and expression of genes within the PI3K/Akt/mTOR pathway in patients with ductal breast cancer of various malignancy levels. We recognized significant correlations between the expression levels of the studied genes. We also performed a bioinformatics analysis of the data available on the international database TCGA and compared them with our own research. Studies on mutations and expression of genes were conducted using High-Resolution Melt PCR (HRM-PCR), Allele-Specific-quantitative PCR (ASP-qPCR), Real-Time PCR molecular methods in a group of women with ductal breast cancer. Bioinformatics analysis was carried out using web source Ualcan and bc-GenExMiner. In the studied group of women, it was observed that the prevalence of mutations in the studied PIK3CA and AKT1 genes was 29.63%. It was stated that the average expression level of the PIK3CA, PIK3R1, PTEN genes in the group of breast cancer patients is lower in comparison to the control group, while the average expression level of the AKT1 and mTOR genes in the studied group was higher in comparison to the control group. It was also indicated that in the group of patients with mutations in the area of the PIK3CA and AKT1 genes, the PIK3CA gene expression level is statistically significantly lower than in the group without mutations. According to our knowledge, we demonstrate, for the first time, that there is a very strong positive correlation between the levels of AKT1 and mTOR gene expression in the case of patients with mutations and without mutations.
Przemysław Kołodziej; Marcin Nicoś; Paweł A. Krawczyk; Jacek Bogucki; Agnieszka Karczmarczyk; Daniel Zalewski; Tomasz Kubrak; Elżbieta Kołodziej; Anna Makuch-Kocka; Barbara Madej-Czerwonka; Bartosz J. Płachno; Janusz Kocki; Anna Bogucka-Kocka. The Correlation of Mutations and Expressions of Genes within the PI3K/Akt/mTOR Pathway in Breast Cancer—A Preliminary Study. International Journal of Molecular Sciences 2021, 22, 2061 .
AMA StylePrzemysław Kołodziej, Marcin Nicoś, Paweł A. Krawczyk, Jacek Bogucki, Agnieszka Karczmarczyk, Daniel Zalewski, Tomasz Kubrak, Elżbieta Kołodziej, Anna Makuch-Kocka, Barbara Madej-Czerwonka, Bartosz J. Płachno, Janusz Kocki, Anna Bogucka-Kocka. The Correlation of Mutations and Expressions of Genes within the PI3K/Akt/mTOR Pathway in Breast Cancer—A Preliminary Study. International Journal of Molecular Sciences. 2021; 22 (4):2061.
Chicago/Turabian StylePrzemysław Kołodziej; Marcin Nicoś; Paweł A. Krawczyk; Jacek Bogucki; Agnieszka Karczmarczyk; Daniel Zalewski; Tomasz Kubrak; Elżbieta Kołodziej; Anna Makuch-Kocka; Barbara Madej-Czerwonka; Bartosz J. Płachno; Janusz Kocki; Anna Bogucka-Kocka. 2021. "The Correlation of Mutations and Expressions of Genes within the PI3K/Akt/mTOR Pathway in Breast Cancer—A Preliminary Study." International Journal of Molecular Sciences 22, no. 4: 2061.
The BIRC (baculoviral IAP repeat‐containing; BIRC) family genes encode for Inhibitor of Apoptosis (IAP) proteins. The dysregulation of the expression levels of the genes in question in cancer tissue as compared to normal tissue suggests that the apoptosis process in cancer cells was disturbed, which may be associated with the development and chemoresistance of triple negative breast cancer (TNBC). In our study, we determined the expression level of eight genes from the BIRC family using the Real-Time PCR method in patients with TNBC and compared the obtained results with clinical data. Additionally, using bioinformatics tools (Ualcan and The Breast Cancer Gene-Expression Miner v4.5 (bc-GenExMiner v4.5)), we compared our data with the data in the Cancer Genome Atlas (TCGA) database. We observed diverse expression pattern among the studied genes in breast cancer tissue. Comparing the expression level of the studied genes with the clinical data, we found that in patients diagnosed with breast cancer under the age of 50, the expression levels of all studied genes were higher compared to patients diagnosed after the age of 50. We observed that in patients with invasion of neoplastic cells into lymphatic vessels and fat tissue, the expression levels of BIRC family genes were lower compared to patients in whom these features were not noted. Statistically significant differences in gene expression were also noted in patients classified into three groups depending on the basis of the Scarff-Bloom and Richardson (SBR) Grading System.
Anna Makuch-Kocka; Janusz Kocki; Anna Brzozowska; Jacek Bogucki; Przemysław Kołodziej; Bartosz J. Płachno; Anna Bogucka-Kocka. The BIRC Family Genes Expression in Patients with Triple Negative Breast Cancer. International Journal of Molecular Sciences 2021, 22, 1820 .
AMA StyleAnna Makuch-Kocka, Janusz Kocki, Anna Brzozowska, Jacek Bogucki, Przemysław Kołodziej, Bartosz J. Płachno, Anna Bogucka-Kocka. The BIRC Family Genes Expression in Patients with Triple Negative Breast Cancer. International Journal of Molecular Sciences. 2021; 22 (4):1820.
Chicago/Turabian StyleAnna Makuch-Kocka; Janusz Kocki; Anna Brzozowska; Jacek Bogucki; Przemysław Kołodziej; Bartosz J. Płachno; Anna Bogucka-Kocka. 2021. "The BIRC Family Genes Expression in Patients with Triple Negative Breast Cancer." International Journal of Molecular Sciences 22, no. 4: 1820.
External apical root resorption (EARR) is one of the most serious complications associated with orthodontic treatment. The aim of the study was to analyze the relationships between selected single nucleotide polymorphisms (SNPs) in Interleukin 1 receptor antagonist (IL1RN), purinoreceptor P2X7 (P2RX7) and EARR in patients after orthodontic treatment. The study comprised 101 patients who underwent a complex orthodontic treatment with a combination of fixed appliances. Roots were measured based on orthopantomograms and lateral cephalometric radiographs taken before and at the end of the treatment using diagnostic software. Proportional measurements of selected teeth were made using the modified Linge and Linge methods. Based on the presence or absence of EARR, patients were divided into two groups: control group, 61 patients without EARR (with 0.90 ≤ rRCR ≤ 1.00), and EARR group, 40 patients with EARR (rRCR < 0.90). Root resorption in selected groups was also evaluated with the scores of Malmgren and Levander. SNP analysis was performed using the real-time polymerase chain reaction (PCR) method. The analysis indicated that a specific haplotype of P2RX7 (rs208294) and IL1RN (rs419598) modified the risk of EARR development (p < 0.05), with a Bonferroni correction. The analysis of the P2RX7 and IL1RN gene polymorphisms showed that the presence of SNPs of these genes may predispose individuals to EARR. These findings indicate that EARR is a complex condition influenced not only by environmental factors and needs further study on the genetic risk factors.
Agata Ciurla; Jolanta Szymańska; Bartosz Płachno; Anna Bogucka-Kocka. Polymorphisms of Encoding Genes IL1RN and P2RX7 in Apical Root Resorption in Patients after Orthodontic Treatment. International Journal of Molecular Sciences 2021, 22, 777 .
AMA StyleAgata Ciurla, Jolanta Szymańska, Bartosz Płachno, Anna Bogucka-Kocka. Polymorphisms of Encoding Genes IL1RN and P2RX7 in Apical Root Resorption in Patients after Orthodontic Treatment. International Journal of Molecular Sciences. 2021; 22 (2):777.
Chicago/Turabian StyleAgata Ciurla; Jolanta Szymańska; Bartosz Płachno; Anna Bogucka-Kocka. 2021. "Polymorphisms of Encoding Genes IL1RN and P2RX7 in Apical Root Resorption in Patients after Orthodontic Treatment." International Journal of Molecular Sciences 22, no. 2: 777.
Purpose Demodex mites infestation, typically asymptomatic, is a problem for patients with weakened immune systems because it often takes the form of symptomatic, massive infection. The Demodex mites play an important role in the occurrence of a range of eye surface diseases such as Demodex blepharitis, Meibomian gland dysfunctions, conjunctivitis and corneal changes. The ocular infection is closely related to the systemic invasion. Our goal was to minimize infestation and alleviate the symptoms of massive demodicosis so as to prevent further damage to the cornea. Methods Our research note involves a 61-year old woman diagnosed with secondary Sjögren syndrome due to rheumatoid arthritis. On the background of the autoimmune disease, corneal perforation of the left eye occurred that was cured by surgery. Then during the follow-up visit the patient was found (microscopically) massively infected with Demodex mites and the developed symptoms were particularly severe. Results Adequate dry eye syndrome and massive demodicosis therapy significantly reduced the number of Demodex mites and improved the patient’s condition. Conclusion We would like to draw the attention of the physicians of different specialties that special care should be taken with respect to the therapy of dry eye syndrome and ocular demodicosis in patients with immunological disorders to achieve therapeutic success and avoid particularly dangerous consequences of these diseases.
Marta Ziaja-Sołtys; Magdalena Kołodziejczyk; Beata Rymgayłło-Jankowska; Dominika Wróbel-Dudzińska; Ewa Suchodoła-Ratajewicz; Dominika Szlonzak; Tomasz Żarnowski; Anna Bogucka-Kocka. Massive Demodicosis of the Eyes in a Patient with Sjögren Syndrome: A Case Report. Acta Parasitologica 2020, 66, 677 -681.
AMA StyleMarta Ziaja-Sołtys, Magdalena Kołodziejczyk, Beata Rymgayłło-Jankowska, Dominika Wróbel-Dudzińska, Ewa Suchodoła-Ratajewicz, Dominika Szlonzak, Tomasz Żarnowski, Anna Bogucka-Kocka. Massive Demodicosis of the Eyes in a Patient with Sjögren Syndrome: A Case Report. Acta Parasitologica. 2020; 66 (2):677-681.
Chicago/Turabian StyleMarta Ziaja-Sołtys; Magdalena Kołodziejczyk; Beata Rymgayłło-Jankowska; Dominika Wróbel-Dudzińska; Ewa Suchodoła-Ratajewicz; Dominika Szlonzak; Tomasz Żarnowski; Anna Bogucka-Kocka. 2020. "Massive Demodicosis of the Eyes in a Patient with Sjögren Syndrome: A Case Report." Acta Parasitologica 66, no. 2: 677-681.
The aim of this study was to evaluate the ability of multivariate techniques to predict antioxidant and cytotoxic activity of the selected lichens from the chromatographic data. A simple and reproducible HPLC-DAD technique has been used to obtain the chromatographic fingerprint profiles. Reversed phase high performance liquid chromatography (RP-HPLC) linear gradient system with methanol, water and phosphoric acid (V) (pH 2.3) as the mobile phase was used (50 min). Principal Component Analysis (PCA) has been applied to the evaluation of the phytochemical similarity between studied samples, especially between the same species collected in various places of Poland (Cetraria islandica (L.) Ach., CI, Cladina mitis Sandst., CM, Hypogymnia physodes (L.) Nyl., HP). The ability to scavenge free radicals was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods and the total phenolic content was determined by Folin-Ciocalteu (F-C) test. In the case of DPPH % of inhibition was higher for selected species (Pseudevernia furfuracea (L.) Zopf, H. physodes in comparison to the literature data. The FRAP test showed that the H. physodes extract had higher ability to scavenge free radical in comparison to Cladonia furcata (Huds.) Schrader and Evernia prunastri (L.) Ach., whereas P. furfuracea extract showed higher ability than C. islandica. The high content of phenolics in P. furfuracea and H. physodes confirms their high antioxidant activity. The cytotoxic activity of studied extracts was tested by cell culture method using the human HL-60 / MX2 acute CKL-22 (CRL-2257) promyelocytic leukemia tumor cell line. The lowest values of IC50 [µg∙mL−1] were obtained for: H. physodes (HP1)—99.4; C. digitate—122.6; H. physodes (HP)—136.5, C. subulata—142.6; C. mitis—180.2.
Anna Hawrył; Mirosław Hawrył; Agnieszka Hajnos-Stolarz; Jagoda Abramek; Anna Bogucka-Kocka; Łukasz Komsta. HPLC Fingerprint Analysis with the Antioxidant and Cytotoxic Activities of Selected Lichens Combined with the Chemometric Calculations. Molecules 2020, 25, 4301 .
AMA StyleAnna Hawrył, Mirosław Hawrył, Agnieszka Hajnos-Stolarz, Jagoda Abramek, Anna Bogucka-Kocka, Łukasz Komsta. HPLC Fingerprint Analysis with the Antioxidant and Cytotoxic Activities of Selected Lichens Combined with the Chemometric Calculations. Molecules. 2020; 25 (18):4301.
Chicago/Turabian StyleAnna Hawrył; Mirosław Hawrył; Agnieszka Hajnos-Stolarz; Jagoda Abramek; Anna Bogucka-Kocka; Łukasz Komsta. 2020. "HPLC Fingerprint Analysis with the Antioxidant and Cytotoxic Activities of Selected Lichens Combined with the Chemometric Calculations." Molecules 25, no. 18: 4301.
Abdominal artery aneurysm (AAA) refers to abdominal aortic dilatation of 3 cm or greater. AAA is frequently underdiagnosed due to often asymptomatic character of the disease, leading to elevated mortality due to aneurysm rupture. MiRNA constitute a pool of small RNAs controlling gene expression and is involved in many pathologic conditions in human. Targeted panel detecting altered expression of miRNA and genes involved in AAA would improve early diagnosis of this disease. In the presented study, we selected and analyzed miRNA and gene expression signatures in AAA patients. Next, generation sequencing was applied to obtain miRNA and gene-wide expression profiles from peripheral blood mononuclear cells in individuals with AAA and healthy controls. Differential expression analysis was performed using DESeq2 and uninformative variable elimination by partial least squares (UVE-PLS) methods. A total of 31 miRNAs and 51 genes were selected as the most promising biomarkers of AAA. Receiver operating characteristics (ROC) analysis showed good diagnostic ability of proposed biomarkers. Genes regulated by selected miRNAs were determined in silico and associated with functional terms closely related to cardiovascular and neurological diseases. Proposed biomarkers may be used for new diagnostic and therapeutic approaches in management of AAA. The findings will also contribute to the pool of knowledge about miRNA-dependent regulatory mechanisms involved in pathology of that disease.
Daniel Zalewski; Karol Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Łukasz Komsta; Przemysław Kołodziej; Paulina Chmiel; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. Dysregulation of microRNA Modulatory Network in Abdominal Aortic Aneurysm. Journal of Clinical Medicine 2020, 9, 1974 .
AMA StyleDaniel Zalewski, Karol Ruszel, Andrzej Stępniewski, Dariusz Gałkowski, Jacek Bogucki, Łukasz Komsta, Przemysław Kołodziej, Paulina Chmiel, Tomasz Zubilewicz, Marcin Feldo, Janusz Kocki, Anna Bogucka-Kocka. Dysregulation of microRNA Modulatory Network in Abdominal Aortic Aneurysm. Journal of Clinical Medicine. 2020; 9 (6):1974.
Chicago/Turabian StyleDaniel Zalewski; Karol Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Łukasz Komsta; Przemysław Kołodziej; Paulina Chmiel; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. 2020. "Dysregulation of microRNA Modulatory Network in Abdominal Aortic Aneurysm." Journal of Clinical Medicine 9, no. 6: 1974.
Parasitic infections caused by different species of intestinal helminths still poses a threat to public health. There is a need to search for new, effective anthelmintic drugs. A series of novel thiosemicarbazides were synthesized and evaluated for their in vitro anthelmintic activity. The preliminary results showed that the most of synthesized compounds were very active. 4-Phenyl-1-[(1-methyl-4-nitroimidazol-2-yl)carbonyl]thiosemicarbazide and 4-(3-chlorophenyl)-1-[(1-methyl-4-nitroimidazol-2-yl)carbonyl]thiosemicarbazide showed a 100% mortality of nematodes and a high anthelmintic activity in both tested concentrations.
Katarzyna Dziduch; Przemysław Kołodziej; Agata Paneth; Anna Bogucka-Kocka; Monika Wujec. Synthesis and Anthelmintic Activity of New Thiosemicarbazide Derivatives—A Preliminary Study. Molecules 2020, 25, 2770 .
AMA StyleKatarzyna Dziduch, Przemysław Kołodziej, Agata Paneth, Anna Bogucka-Kocka, Monika Wujec. Synthesis and Anthelmintic Activity of New Thiosemicarbazide Derivatives—A Preliminary Study. Molecules. 2020; 25 (12):2770.
Chicago/Turabian StyleKatarzyna Dziduch; Przemysław Kołodziej; Agata Paneth; Anna Bogucka-Kocka; Monika Wujec. 2020. "Synthesis and Anthelmintic Activity of New Thiosemicarbazide Derivatives—A Preliminary Study." Molecules 25, no. 12: 2770.
Background Acute myeloid leukemia (AML) is a heterogenic lethal disorder characterized by the accumulation of abnormal myeloid progenitor cells in the bone marrow which results in hematopoietic failure. Despite various efforts in detection and treatment, many patients with AML die of this cancer. That is why it is important to develop novel therapeutic options, employing strategic target genes involved in apoptosis and tumor progression. Methods The aim of the study was to evaluate PARP1, PARP2, PARP3, and TRPM2 gene expression at mRNA level using qPCR method in the cells of hematopoietic system of the bone marrow in patients with acute myeloid leukemia, bone marrow collected from healthy patients, peripheral blood of healthy individuals, and hematopoietic stem cells from the peripheral blood after mobilization. Results The results found that the bone marrow cells of the patients with acute myeloid leukemia (AML) show overexpression of PARP1 and PARP2 genes and decreased TRPM2 gene expression. In the hematopoietic stem cells derived from the normal marrow and peripheral blood after mobilization, the opposite situation was observed, i.e. TRPM2 gene showed increased expression while PARP1 and PARP2 gene expression was reduced. We observed positive correlations between PARP1, PARP2, PARP3, and TRPM2 genes expression in the group of mature mononuclear cells derived from the peripheral blood and in the group of bone marrow-derived cells. In AML cells significant correlations were not observed between the expression of the examined genes. In addition, we observed that the reduced expression of TRPM2 and overexpression of PARP1 are associated with a shorter overall survival of patients, indicating the prognostic significance of these genes expression in AML. Conclusions Our research suggests that in physiological conditions in the cells of the hematopoietic system there is mutual positive regulation of PARP1, PARP2, PARP3, and TRPM2 genes expression. PARP1, PARP2, and TRPM2 genes at mRNA level deregulate in acute myeloid leukemia cells.
Paulina Gil-Kulik; Ewa Dudzińska; Elżbieta Radzikowska-Büchner; Joanna Wawer; Mariusz Jojczuk; Adam Nogalski; Genowefa Anna Wawer; Marcin Feldo; Wojciech Kocki; Maria Cioch; Anna Bogucka-Kocka; Mansur Rahnama; Janusz Kocki. Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells. BMC Cancer 2020, 20, 1 -9.
AMA StylePaulina Gil-Kulik, Ewa Dudzińska, Elżbieta Radzikowska-Büchner, Joanna Wawer, Mariusz Jojczuk, Adam Nogalski, Genowefa Anna Wawer, Marcin Feldo, Wojciech Kocki, Maria Cioch, Anna Bogucka-Kocka, Mansur Rahnama, Janusz Kocki. Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells. BMC Cancer. 2020; 20 (1):1-9.
Chicago/Turabian StylePaulina Gil-Kulik; Ewa Dudzińska; Elżbieta Radzikowska-Büchner; Joanna Wawer; Mariusz Jojczuk; Adam Nogalski; Genowefa Anna Wawer; Marcin Feldo; Wojciech Kocki; Maria Cioch; Anna Bogucka-Kocka; Mansur Rahnama; Janusz Kocki. 2020. "Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells." BMC Cancer 20, no. 1: 1-9.
Chronic venous disease (CVD) is a vascular disease of lower limbs with high prevalence worldwide. Pathologic features include varicose veins, venous valves dysfunction and skin ulceration resulting from dysfunction of cell proliferation, apoptosis and angiogenesis. These processes are partly regulated by microRNA (miRNA)-dependent modulation of gene expression, pointing to miRNA as a potentially important target in diagnosis and therapy of CVD progression. The aim of the study was to analyze alterations of miRNA and gene expression in CVD, as well as to identify miRNA-mediated changes in gene expression and their potential link to CVD development. Using next generation sequencing, miRNA and gene expression profiles in peripheral blood mononuclear cells of subjects with CVD in relation to healthy controls were studied. Thirty-one miRNAs and 62 genes were recognized as potential biomarkers of CVD using DESeq2, Uninformative Variable Elimination by Partial Least Squares (UVE-PLS) and ROC (Receiver Operating Characteristics) methods. Regulatory interactions between potential biomarker miRNAs and genes were projected. Functional analysis of microRNA-regulated genes revealed terms closely related to cardiovascular diseases and risk factors. The study shed new light on miRNA-dependent regulatory mechanisms involved in the pathology of CVD. MicroRNAs and genes proposed as CVD biomarkers may be used to develop new diagnostic and therapeutic methods.
Daniel P. Zalewski; Karol P. Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Łukasz Komsta; Przemysław Kołodziej; Paulina Chmiel; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. Dysregulations of MicroRNA and Gene Expression in Chronic Venous Disease. Journal of Clinical Medicine 2020, 9, 1251 .
AMA StyleDaniel P. Zalewski, Karol P. Ruszel, Andrzej Stępniewski, Dariusz Gałkowski, Jacek Bogucki, Łukasz Komsta, Przemysław Kołodziej, Paulina Chmiel, Tomasz Zubilewicz, Marcin Feldo, Janusz Kocki, Anna Bogucka-Kocka. Dysregulations of MicroRNA and Gene Expression in Chronic Venous Disease. Journal of Clinical Medicine. 2020; 9 (5):1251.
Chicago/Turabian StyleDaniel P. Zalewski; Karol P. Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Łukasz Komsta; Przemysław Kołodziej; Paulina Chmiel; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki; Anna Bogucka-Kocka. 2020. "Dysregulations of MicroRNA and Gene Expression in Chronic Venous Disease." Journal of Clinical Medicine 9, no. 5: 1251.
The leaves of Pyrola rotundifolia L. were extracted in the mixed solvent of methanol/acetone/water (2:2:1, v/v/v) and investigated for their phytochemical analysis and biological activity. Total phenolic and flavonoid contents were determined spectrophotometrically. A high content of phenols (208.35 mg GAE/g of dry extract), flavonoids (38.90 mg QE/g of dry extract) and gallotannins (722.91 GAE/g of dry extract) was obtained. Ultra-high performance liquid chromatography diode array detector tandem mass spectrometry (UHPLC–DAD–MS) allowed for the detection of 23 major peaks at 254 nm. The extract was analyzed for its antioxidant capacity using 2,2-diphenyl-1-picryl-hydrazyl (DPPH•) and 2,2′-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS•+) radical scavenging, metal chelating power and β-carotene-linoleic acid bleaching assays. The examined extract showed moderate radical scavenging and chelating activity, and good inhibiting ability of linoleic acid oxidation (EC50 = 0.05 mg/mL) in comparison to standards. The cytotoxic effect in increasing concentration on five types of leukemic cell lines was also investigated using trypan blue vital staining. It was found that the analyzed extract induced the apoptosis of all the tested cell lines. Our findings suggest that the leaves of P. rotundifolia are a source of valuable compounds providing protection against oxidative damage, hence their use in traditional medicine is justified.
Katarzyna Szewczyk; Anna Bogucka-Kocka; Natalia Vorobets; Anna Grzywa-Celińska; Sebastian Granica. Phenolic Composition of the Leaves of Pyrola rotundifolia L. and Their Antioxidant and Cytotoxic Activity. Molecules 2020, 25, 1749 .
AMA StyleKatarzyna Szewczyk, Anna Bogucka-Kocka, Natalia Vorobets, Anna Grzywa-Celińska, Sebastian Granica. Phenolic Composition of the Leaves of Pyrola rotundifolia L. and Their Antioxidant and Cytotoxic Activity. Molecules. 2020; 25 (7):1749.
Chicago/Turabian StyleKatarzyna Szewczyk; Anna Bogucka-Kocka; Natalia Vorobets; Anna Grzywa-Celińska; Sebastian Granica. 2020. "Phenolic Composition of the Leaves of Pyrola rotundifolia L. and Their Antioxidant and Cytotoxic Activity." Molecules 25, no. 7: 1749.
Background Acute myeloid leukemia (AML) is a heterogenic lethal disorder characterized by the accumulation of abnormal myeloid progenitor cells in the bone marrow which results in hematopoietic failure. Despite various efforts in detection and treatment, many patients with AML die of this cancer. That is why it is important to develop novel therapeutic options, employing strategic target genes involved in apoptosis and tumor progression. Methods The aim of the study was to evaluate PARP1, PARP2, PARP3, and TRPM2 gene expression at mRNA level using qPCR method in the cells of hematopoietic system of the bone marrow in patients with acute myeloid leukemia, bone marrow collected from healthy patients, peripheral blood of healthy individuals, and hematopoietic stem cells from the peripheral blood after mobilization. Results The results found that the bone marrow cells of the patients with acute myeloid leukemia (AML) show overexpression of PARP1 and PARP2 genes and decreased TRPM2 gene expression. In the hematopoietic stem cells derived from the normal marrow and peripheral blood after mobilization, the opposite situation was observed, i.e. TRPM2 gene showed increased expression while PARP1 and PARP2 gene expression was reduced. We observed positive correlations between PARP1, PARP2, PARP3, and TRPM2 genes expression in the group of mature mononuclear cells derived from the peripheral blood and in the group of bone marrow-derived cells. In AML cells significant correlations were not observed between the expression of the examined genes. In addition, we observed that the reduced expression of TRPM2 and overexpression of PARP1 are associated with a shorter overall survival of patients, indicating the prognostic significance of these genes expression in AML. Conclusions Our research suggests that in physiological conditions in the cells of the hematopoietic system there is mutual positive regulation of PARP1, PARP2, PARP3, and TRPM2 genes expression. PARP1, PARP2, and TRPM2 genes at mRNA level deregulate in acute myeloid leukemia cells.
Paulina Gil-Kulik; Ewa Dudzińska; Elżbieta Radzikowska-Büchner; Joanna Wawer; Mariusz Jojczuk; Adam Nogalski; Genowefa Anna Wawer; Marcin Feldo; Wojciech Kocki; Maria Cioch; Anna Bogucka-Kocka; Mansur Rahnama; Janusz Kocki. Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells. 2020, 1 .
AMA StylePaulina Gil-Kulik, Ewa Dudzińska, Elżbieta Radzikowska-Büchner, Joanna Wawer, Mariusz Jojczuk, Adam Nogalski, Genowefa Anna Wawer, Marcin Feldo, Wojciech Kocki, Maria Cioch, Anna Bogucka-Kocka, Mansur Rahnama, Janusz Kocki. Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells. . 2020; ():1.
Chicago/Turabian StylePaulina Gil-Kulik; Ewa Dudzińska; Elżbieta Radzikowska-Büchner; Joanna Wawer; Mariusz Jojczuk; Adam Nogalski; Genowefa Anna Wawer; Marcin Feldo; Wojciech Kocki; Maria Cioch; Anna Bogucka-Kocka; Mansur Rahnama; Janusz Kocki. 2020. "Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells." , no. : 1.
Anna Hawrył; Mirosław Hawrył; Wojciech Litwińczuk; Anna Bogucka-Kocka. Thin-layer chromatographic fingerprint of selected Paulownia species with chemometrics and antioxidant activity. Journal of Liquid Chromatography & Related Technologies 2020, 43, 367 -374.
AMA StyleAnna Hawrył, Mirosław Hawrył, Wojciech Litwińczuk, Anna Bogucka-Kocka. Thin-layer chromatographic fingerprint of selected Paulownia species with chemometrics and antioxidant activity. Journal of Liquid Chromatography & Related Technologies. 2020; 43 (11-12):367-374.
Chicago/Turabian StyleAnna Hawrył; Mirosław Hawrył; Wojciech Litwińczuk; Anna Bogucka-Kocka. 2020. "Thin-layer chromatographic fingerprint of selected Paulownia species with chemometrics and antioxidant activity." Journal of Liquid Chromatography & Related Technologies 43, no. 11-12: 367-374.
Water soluble polysaccharides (WSP) were isolated from Lentinus edodes fruiting bodies. The mushrooms were previously subjected to various processing techniques which included blanching, boiling, and fermenting with lactic acid bacteria. Therefore, the impact of processing on the content and biological activities of WSP was established. Non-processed fruiting bodies contained 10.70 ± 0.09 mg/g fw. Boiling caused ~12% decrease in the amount of WSP, while blanched and fermented mushrooms showed ~6% decline. Fourier transform infrared spectroscopy analysis (FTIR) confirmed the presence of β-glycosidic links, whereas due to size exclusion chromatography 216 kDa and 11 kDa molecules were detected. WSP exhibited antioxidant potential in FRAP (ferric ion reducing antioxidant power) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays. Cytotoxic properties were determined on MCF-7 and T47D human breast cell lines using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) test. Both biological activities decreased as the result of boiling and fermenting.
Marta Ziaja-Sołtys; Wojciech Radzki; Jakub Nowak; Jolanta Topolska; Ewa Jabłońska-Ryś; Aneta Sławińska; Katarzyna Skrzypczak; Andrzej Kuczumow; Anna Bogucka-Kocka. Processed Fruiting Bodies of Lentinus edodes as a Source of Biologically Active Polysaccharides. Applied Sciences 2020, 10, 470 .
AMA StyleMarta Ziaja-Sołtys, Wojciech Radzki, Jakub Nowak, Jolanta Topolska, Ewa Jabłońska-Ryś, Aneta Sławińska, Katarzyna Skrzypczak, Andrzej Kuczumow, Anna Bogucka-Kocka. Processed Fruiting Bodies of Lentinus edodes as a Source of Biologically Active Polysaccharides. Applied Sciences. 2020; 10 (2):470.
Chicago/Turabian StyleMarta Ziaja-Sołtys; Wojciech Radzki; Jakub Nowak; Jolanta Topolska; Ewa Jabłońska-Ryś; Aneta Sławińska; Katarzyna Skrzypczak; Andrzej Kuczumow; Anna Bogucka-Kocka. 2020. "Processed Fruiting Bodies of Lentinus edodes as a Source of Biologically Active Polysaccharides." Applied Sciences 10, no. 2: 470.
Acute myeloid leukemia (AML) is a heterogenic lethal disorder characterized by the accumulation of abnormal myeloid progenitor cells in the bone marrow, which results in hematopoietic failure. Despite various efforts in detection and treatment, many patients with AML die of this cancer. That is why it is important to develop novel therapeutic options, employing strategic target genes involved in apoptosis and tumor progression. The aim of the study was to evaluate PARP1, PARP2, PARP3, and TRPM2 gene expression at the mRNA level in the cells of the hematopoietic system of the bone marrow in patients with acute myeloid leukemia, bone marrow collected from healthy patients, peripheral blood of healthy individuals, and hematopoietic stem cells from the peripheral blood after mobilization.Results: The results found that the bone marrow cells of patients with acute myeloid leukemia (AML) show over expression of PARP1 and PARP2 genes and decreased TRPM2 gene expression. In the hematopoietic stem cells derived from the normal marrow and peripheral blood after mobilization, the opposite situation was observed, i.e. TRPM2 gene showed increased expression while PARP1 and PARP2 gene expression was reduced. We observed the positive correlations between PARP1, PARP2, PARP3, and TRPM2 genes expression in the group of mature mononuclear cells derived from the peripheral blood and in the group of bone marrow-derived cells. In AML cells significant correlations were not observed between the expression of the examined genes.Conclusions: Our research suggests that in physiological conditions in the cells of the hematopoietic system there is mutual positive regulation of PARP1, PARP2, PARP3, and TRPM2 genes expression. PARP1, PARP2, and TRPM2 genes at mRNA level deregulate in acute myeloid leukemia cells.
Paulina Gil-Kulik; Ewa Dudzińska; Elżbieta Radzikowska-Büchner; Joanna Wawer; Mariusz Jojczuk; Adam Nogalski; Genowefa Anna Wawer; Marcin Feldo; Wojciech Kocki; Maria Cioch; Anna Bogucka-Kocka; Mansur Rahnama; Janusz Kocki. Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells. 2019, 1 .
AMA StylePaulina Gil-Kulik, Ewa Dudzińska, Elżbieta Radzikowska-Büchner, Joanna Wawer, Mariusz Jojczuk, Adam Nogalski, Genowefa Anna Wawer, Marcin Feldo, Wojciech Kocki, Maria Cioch, Anna Bogucka-Kocka, Mansur Rahnama, Janusz Kocki. Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells. . 2019; ():1.
Chicago/Turabian StylePaulina Gil-Kulik; Ewa Dudzińska; Elżbieta Radzikowska-Büchner; Joanna Wawer; Mariusz Jojczuk; Adam Nogalski; Genowefa Anna Wawer; Marcin Feldo; Wojciech Kocki; Maria Cioch; Anna Bogucka-Kocka; Mansur Rahnama; Janusz Kocki. 2019. "Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells." , no. : 1.
There is currently no knowledge about the expression profile of the autophagy (BECN1), mitophagy (BNIP3), and apoptosis (CASP3) genes in the CA3 region of the hippocampus after cerebral ischemia. In addition, it is unknown whether genes for BECN1, BNIP3, and CASP3 have any effect on the neuronal death in the CA3 area of the hippocampus due to ischemia. In this study, for the first time, we present, by means of a quantitative PCR protocol with reverse transcriptase, the expression of BECN1 and CASP3 genes in the neuronal CA3 region of the hippocampus with the co-expression of the mitochondrial BNIP3 gene, which genes are associated with Alzheimer's disease, in the ischemic model of Alzheimer's disease in the rat. The present study showed that after ischemia, the CASP3 gene was significantly expressed within 7-30 days, the BECN1 gene was significantly overexpressed on the thirtieth day, and the BINP3 gene was lowered below control values during post-ischemic follow-up period. The caspase-dependent neuronal death in the CA3 region of the hippocampus after ischemia is not accompanied by overexpression of the BNIP3 gene. Our data may therefore suggest a new insight into the BNIP3 gene in the regulation of neuronal mitophagy in neurodegeneration in the CA3 region of the hippocampus after ischemia. This indicates no involvement of the BNIP3 gene along with the CASP3 gene in the CA3 region of the hippocampus in delayed neuronal death after brain ischemia.
Marzena Ułamek-Kozioł; Stanisław J. Czuczwar; Janusz Kocki; Sławomir Januszewski; Jacek Bogucki; Anna Bogucka-Kocka; Ryszard Pluta. Dysregulation of Autophagy, Mitophagy, and Apoptosis Genes in the CA3 Region of the Hippocampus in the Ischemic Model of Alzheimer’s Disease in the Rat. Journal of Alzheimer's Disease 2019, 72, 1279 -1286.
AMA StyleMarzena Ułamek-Kozioł, Stanisław J. Czuczwar, Janusz Kocki, Sławomir Januszewski, Jacek Bogucki, Anna Bogucka-Kocka, Ryszard Pluta. Dysregulation of Autophagy, Mitophagy, and Apoptosis Genes in the CA3 Region of the Hippocampus in the Ischemic Model of Alzheimer’s Disease in the Rat. Journal of Alzheimer's Disease. 2019; 72 (4):1279-1286.
Chicago/Turabian StyleMarzena Ułamek-Kozioł; Stanisław J. Czuczwar; Janusz Kocki; Sławomir Januszewski; Jacek Bogucki; Anna Bogucka-Kocka; Ryszard Pluta. 2019. "Dysregulation of Autophagy, Mitophagy, and Apoptosis Genes in the CA3 Region of the Hippocampus in the Ischemic Model of Alzheimer’s Disease in the Rat." Journal of Alzheimer's Disease 72, no. 4: 1279-1286.
Atherosclerosis and its comorbidities are the major contributors to the global burden of death worldwide. Lower extremities arterial disease (LEAD) is a common manifestation of atherosclerotic disease of arteries of lower extremities. MicroRNAs belong to epigenetic factors that regulate gene expression and have not yet been extensively studied in LEAD. We aimed to indicate the most promising microRNA and gene expression signatures of LEAD, to identify interactions between microRNA and genes and to describe potential effect of modulated gene expression. High-throughput sequencing was employed to examine microRNAome and transcriptome of peripheral blood mononuclear cells of patients with LEAD, in relation to controls. Statistical significance of microRNAs and genes analysis results was evaluated using DESeq2 and uninformative variable elimination by partial least squares methods. Altered expression of 26 microRNAs (hsa-let-7f-1-3p, hsa-miR-34a-5p, -122-5p, -3591-3p, -34a-3p, -1261, -21-5p, -15a-5p, -548d-5p, -34b-5p, -424-3p, -548aa, -548t-3p, -4423-3p, -196a-5p, -330-3p, -766-3p, -30e-3p, -125b-5p, -1301-3p, -3184-5p, -423-3p, -339-3p, -138-5p, -99a-3p, and -6087) and 14 genes (AK5, CD248, CDS2, FAM129A, FBLN2, GGT1, NOG, NRCAM, PDE7A, RP11-545E17.3, SLC12A2, SLC16A10, SLC4A10, and ZSCAN18) were the most significantly differentially expressed in LEAD group compared to controls. Discriminative value of revealed microRNAs and genes were confirmed by receiver operating characteristic analysis. Dysregulations of 26 microRNAs and 14 genes were used to propose novel biomarkers of LEAD. Regulatory interactions between biomarker microRNAs and genes were studied in silico using R multiMiR package. Functional analysis of genes modulated by proposed biomarker microRNAs was performed using DAVID 6.8 tools and revealed terms closely related to atherosclerosis and, interestingly, the processes involving nervous system. The study provides new insight into microRNA-dependent regulatory mechanisms involved in pathology of LEAD. Proposed microRNA and gene biomarkers of LEAD may provide new diagnostic and therapeutic opportunities.
Anna Bogucka-Kocka; Daniel P. Zalewski; Karol P. Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Łukasz Komsta; Przemysław Kołodziej; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki. Dysregulation of MicroRNA Regulatory Network in Lower Extremities Arterial Disease. Frontiers in Genetics 2019, 10, 1200 .
AMA StyleAnna Bogucka-Kocka, Daniel P. Zalewski, Karol P. Ruszel, Andrzej Stępniewski, Dariusz Gałkowski, Jacek Bogucki, Łukasz Komsta, Przemysław Kołodziej, Tomasz Zubilewicz, Marcin Feldo, Janusz Kocki. Dysregulation of MicroRNA Regulatory Network in Lower Extremities Arterial Disease. Frontiers in Genetics. 2019; 10 ():1200.
Chicago/Turabian StyleAnna Bogucka-Kocka; Daniel P. Zalewski; Karol P. Ruszel; Andrzej Stępniewski; Dariusz Gałkowski; Jacek Bogucki; Łukasz Komsta; Przemysław Kołodziej; Tomasz Zubilewicz; Marcin Feldo; Janusz Kocki. 2019. "Dysregulation of MicroRNA Regulatory Network in Lower Extremities Arterial Disease." Frontiers in Genetics 10, no. : 1200.
Understanding the mechanisms underlying the selective susceptibility to ischemia of the CA3 region is very important to explain the neuropathology of memory loss after brain ischemia. We used a rat model to study changes in gene expression of the amyloid protein precursor and its cleaving enzymes and tau protein in the hippocampal CA3 sector, after transient 10-min global brain ischemia with survival times of 2, 7, and 30 days. The expression of the α-secretase gene was below control values at all times studied. But, the expression of the β-secretase gene was below the control values at 2–7 days after ischemia and the maximal increase in its expression was observed on day 30. Expression of the presenilin 1 gene was significantly elevated above the control values at 2–7 days after ischemia and decreased below the control values at day 30. Expression of the presenilin 2 gene showed an opposite trend to the expression of presenilin 1. Expression of the amyloid protein precursor gene after ischemia was at all times above the control values with a huge significant overexpression on day 7. Additionally, the expression of the tau protein gene was below the control values 2 days after ischemia, but the significant increase in its expression was observed on days 7–30. Data show that brain ischemia activates neuronal changes and death in the CA3 region of the hippocampus in a manner dependent on amyloid and tau protein, thus determining a new and important way to regulate the survival and/or death of ischemic neurons.
Ryszard Pluta; Marzena Ułamek-Kozioł; Janusz Kocki; Jacek Bogucki; Sławomir Januszewski; Anna Bogucka-Kocka; Stanisław J. Czuczwar. Expression of the Tau Protein and Amyloid Protein Precursor Processing Genes in the CA3 Area of the Hippocampus in the Ischemic Model of Alzheimer’s Disease in the Rat. Molecular Neurobiology 2019, 57, 1281 -1290.
AMA StyleRyszard Pluta, Marzena Ułamek-Kozioł, Janusz Kocki, Jacek Bogucki, Sławomir Januszewski, Anna Bogucka-Kocka, Stanisław J. Czuczwar. Expression of the Tau Protein and Amyloid Protein Precursor Processing Genes in the CA3 Area of the Hippocampus in the Ischemic Model of Alzheimer’s Disease in the Rat. Molecular Neurobiology. 2019; 57 (2):1281-1290.
Chicago/Turabian StyleRyszard Pluta; Marzena Ułamek-Kozioł; Janusz Kocki; Jacek Bogucki; Sławomir Januszewski; Anna Bogucka-Kocka; Stanisław J. Czuczwar. 2019. "Expression of the Tau Protein and Amyloid Protein Precursor Processing Genes in the CA3 Area of the Hippocampus in the Ischemic Model of Alzheimer’s Disease in the Rat." Molecular Neurobiology 57, no. 2: 1281-1290.