The development of environmentally adaptive solutions for magnetically actuated microrobots to enable targeted delivery in complex and confined fluid environments presents a significant challenge. Inspired by the natural locomotion of crucian carp, a barbell‐shaped soft microrobot (MBS²M) is proposed. A mechano‐electromagnetic hybrid actuation system is developed to generate oscillating magnetic fields to manipulate the microrobot. The MBS²M can seamlessly transition between three fundamental locomotion modes: fast navigation (FN), high‐precision navigation (HPN), and fixed‐point rotation (FPR). Moreover, the MBS²M can move in reverse without turning. The multimodal locomotion endows the MBS²M's adaptability in diverse environments. It can smoothly pass through confined channels, climb over obstacles, overcome gravity for vertical motion, track complex pathways, traverse viscous environments, overcome low fluid resistance, and navigate complex spaces mimicking in vivo environments. Additionally, the MBS²M is capable of drug loading and release in response to ultrasound excitation. In an ex vivo porcine liver vein, the microrobot demonstrated targeted navigation under ultrasound guidance, showcasing its potential for specialized in vivo tasks.

Exosomes are essential early biomarkers for health monitoring and cancer diagnosis. A prerequisite for further investigation of exosomes is the isolation, which is technically challenging due to the complexity of body fluids. This paper presents the development of an integrated microfluidic chip for exosomes isolation, which combines the traditional immunomagnetic bead-based protocol and the recently emerging microfluidic approach, resulting in benefits from both the high-purity of the former and the automated continuous superiority of the latter. The chip was designed based on an S-shaped micromixer with embedded baffle. The excellent mixing efficiency of this micromixer compared with Y-shaped and S-shaped micromixers was verified by simulation and experiments. The photolithography technique was employed to fabricate the integrated microfluidic chip, and the manufacturing process was elucidated. We finally established an experimental platform for exosomes isolation with the fabricated microfluidic chip built in. Exosomes isolation experiments were conducted using this platform. The distribution and morphology of the isolated exosomes were observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Quantitative size analyses based on transmission electron micrographs indicated that most of the obtained particles were between 30 and 150 nm. Western blot analyses of the isolated exosomes and the serum were conducted to verify the platform’s capability of isolating a certain subpopulation of exosomes corresponding to specified protein markers (CD63). The complete time for isolation of 150 μL serum samples was approximately 50 min, which was highly competitive with the reported existing protocols. Experimental results proved the capacity of the established integrated microfluidic chip for exosomes isolation with high purity, high integrity, and excellent efficiency. The platform can be further developed to make it possible for practical use in clinical applications as a universal exosomes isolation and characterization tool.

As an emerging multifunctional material, Gallium based room temperature liquid metal has attracted a lot of attention for a variety of applications due to its mobility and deformability. However, controlling the motion of a liquid metal droplet accurately still remains unrevealed, which restricts its application in many fields. In this paper we propose a hybrid framework that would control the motion of a liquid metal droplet in a one-dimensional fluidic channel. A dynamic model of a liquid metal droplet immersed in the electrolyte when an electrical field is applied to each end of the channel is discussed first, followed by a setpoint controller designed to calculate the current input needed to drive the liquid metal droplet to its destination with vision feedback. To obtain the desired high-resolution current output, a fast and high-resolution current output power supply will be established by integrating a fast PID controller and a simple programmable DC power supply. The effectiveness of this proposed approach will be verified by controlling a liquid metal droplet so that it reaches its destination inside the PMMA channel. This proposed approach may lead to the development of tiny soft robots, or micro-fluidic systems that can be driven accurately by liquid metal droplets.

A multi-layer microfluidic chip integrates dynamic cell culture, processing, and in situ measurement of mechanical properties.