Unmanned aerial vehicle (UAV)-based multispectral remote sensing effectively monitors agro-ecosystem functioning and predicts crop yield. However, the timing of the remote sensing field campaigns can profoundly impact the accuracy of yield predictions. Little is known on the effects of phenological phases on skills of high-frequency sensing observations used to predict maize yield. It is also unclear how much improvement can be gained using multi-temporal compared to mono-temporal data. We used a systematic scheme to address those gaps employing UAV multispectral observations at nine development stages of maize (from second-leaf to maturity). Next, the spectral and texture indices calculated from the mono-temporal and multi-temporal UAV images were fed into the Random Forest model for yield prediction. Our results indicated that multi-temporal UAV data could remarkably enhance the yield prediction accuracy compared with mono-temporal UAV data (R² increased by 8.1% and RMSE decreased by 27.4%). For single temporal UAV observation, the fourteenth-leaf stage was the earliest suitable time and the milking stage was the optimal observing time to estimate grain yield. For multi-temporal UAV data, the combination of tasseling, silking, milking, and dough stages exhibited the highest yield prediction accuracy (R² = 0.93, RMSE = 0.77 t·ha⁻¹). Furthermore, we found that the Normalized Difference Red Edge Index (NDRE), Green Normalized Difference Vegetation Index (GNDVI), and dissimilarity of the near-infrared image at milking stage were the most promising feature variables for maize yield prediction.

Crop above-ground biomass (AGB) is a key parameter used for monitoring crop growth and predicting yield in precision agriculture. Estimating the crop AGB at a field scale through the use of unmanned aerial vehicles (UAVs) is promising for agronomic application, but the robustness of the methods used for estimation needs to be balanced with practical application. In this study, three UAV remote sensing flight missions (using a multiSPEC-4C multispectral camera, a Micasense RedEdge-M multispectral camera, and an Alpha Series AL3-32 Light Detection and Ranging (LiDAR) sensor onboard three different UAV platforms) were conducted above three long-term experimental plots with different tillage treatments in 2018. We investigated the performances of the multi-source UAV-based 3D point clouds at multi-spatial scales using the traditional multi-variable linear regression model (OLS), random forest (RF), backpropagation neural network (BP), and support vector machine (SVM) methods for accurate AGB estimation. Results showed that crop height (CH) was a robust proxy for AGB estimation, and that high spatial resolution in CH datasets helps to improve maize AGB estimation. Furthermore, the OLS, RF, BP, and SVM methods all maintained an acceptable accuracy for AGB estimation; however, the SVM and RF methods performed slightly more robustly. This study is expected to optimize UAV systems and algorithms for specific agronomic applications.