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This study evaluated the chemical behaviour of Bisphenol S (BPS) and determined its bioaccessibility after human ingestion using a standardised in vitro gastrointestinal digestion protocol and an analytical method based on high-pressure liquid chromatography coupled with a photodiode array and tandem mass spectrometry. The effects of different factors such as gastric pH, enzymes, and food matrix on the solubility and chemical stability of BPS were studied to evaluate their contribution to its bioaccessibility. The results highlighted that BPS was available at the end of the digestion process in the range of 50–80%, and was susceptible to absorption at the intestinal level. The effect of pH was not significant as a single factor. The presence of enzymes slightly decreased the bioaccessibility of BPS in the intestinal phase with gastric pH increase. Additionally, a soy drink reduced BPS bioaccessibility by up to 5% after oral intake. Finally, a few BPS degradation products were found in non-bioaccessible fractions at different pH values.
Antía Lestido-Cardama; Beatriz Millán Sánchez; Raquel Sendón; Ana Rodríguez-Bernaldo de Quirós; Letricia Barbosa-Pereira. Study on the chemical behaviour of Bisphenol S during the in vitro gastrointestinal digestion and its bioaccessibility. Food Chemistry 2021, 367, 130758 .
AMA StyleAntía Lestido-Cardama, Beatriz Millán Sánchez, Raquel Sendón, Ana Rodríguez-Bernaldo de Quirós, Letricia Barbosa-Pereira. Study on the chemical behaviour of Bisphenol S during the in vitro gastrointestinal digestion and its bioaccessibility. Food Chemistry. 2021; 367 ():130758.
Chicago/Turabian StyleAntía Lestido-Cardama; Beatriz Millán Sánchez; Raquel Sendón; Ana Rodríguez-Bernaldo de Quirós; Letricia Barbosa-Pereira. 2021. "Study on the chemical behaviour of Bisphenol S during the in vitro gastrointestinal digestion and its bioaccessibility." Food Chemistry 367, no. : 130758.
The cocoa bean shell (CBS) is one of the main cocoa byproducts with a prospective to be used as a functional food ingredient due to its nutritional and sensory properties. This study aims to define the chemical fingerprint of CBSs obtained from cocoa beans of diverse cultivars and collected in different geographical areas of Venezuela assessed using high-performance liquid chromatography coupled to photodiodes array and mass spectrometry (HPLC-PDA-MS/MS) and spectrophotometric assays combined with multivariate analysis for classification purposes. The study provides a comprehensive fingerprint and quantitative data for 39 compounds, including methylxanthines and several polyphenols, such as flavan-3-ols, procyanidins, and N-phenylpropenoyl amino acids. Several key cocoa markers, such as theobromine, epicatechin, quercetin-3-O-glucoside, procyanidin_A pentoside_3, and N-coumaroyl-l-aspartate_2, were found suitable for the classification of CBS according to their cultivar and origin. Despite the screening methods required a previous purification of the sample, both methodologies appear to be suitable for the classification of CBS with a high correlation between datasets. Finally, preliminary findings on the identification of potential contributors for the radical scavenging activity of CBS were also accomplished to support the valorization of this byproduct as a bioactive ingredient in the production of functional foods.
Letricia Barbosa-Pereira; Simona Belviso; Ilario Ferrocino; Olga Rojo-Poveda; Giuseppe Zeppa. Characterization and Classification of Cocoa Bean Shells from Different Regions of Venezuela Using HPLC-PDA-MS/MS and Spectrophotometric Techniques Coupled to Chemometric Analysis. Foods 2021, 10, 1791 .
AMA StyleLetricia Barbosa-Pereira, Simona Belviso, Ilario Ferrocino, Olga Rojo-Poveda, Giuseppe Zeppa. Characterization and Classification of Cocoa Bean Shells from Different Regions of Venezuela Using HPLC-PDA-MS/MS and Spectrophotometric Techniques Coupled to Chemometric Analysis. Foods. 2021; 10 (8):1791.
Chicago/Turabian StyleLetricia Barbosa-Pereira; Simona Belviso; Ilario Ferrocino; Olga Rojo-Poveda; Giuseppe Zeppa. 2021. "Characterization and Classification of Cocoa Bean Shells from Different Regions of Venezuela Using HPLC-PDA-MS/MS and Spectrophotometric Techniques Coupled to Chemometric Analysis." Foods 10, no. 8: 1791.
Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are synthetic chemical compounds widely used in different industry fields including food contact materials (FCM), providing resistance to fat and humidity, and non-stick properties. PFAS enter into the food chain directly from the intake of contaminated food or indirectly from the migration of the FCM into the food. This exposure published in different research highlights a public health concern. Therefore, it is necessary to perform analysis of the content of different FCM and evaluate the migration from the FCM under normal conditions of use and storage. This bibliographical review proves that different perfluoroalkyl and polyfluoroalkyl compounds are detected in fast food packaging, microwave popcorn bags, and frying pans, among others. Furthermore, it shows the conditions or factors that favor the migration of the PFAS from the FCM into the food.
Arabela Ramírez Carnero; Antía Lestido-Cardama; Patricia Vazquez Loureiro; Letricia Barbosa-Pereira; Ana Rodríguez Bernaldo de Quirós; Raquel Sendón. Presence of Perfluoroalkyl and Polyfluoroalkyl Substances (PFAS) in Food Contact Materials (FCM) and Its Migration to Food. Foods 2021, 10, 1443 .
AMA StyleArabela Ramírez Carnero, Antía Lestido-Cardama, Patricia Vazquez Loureiro, Letricia Barbosa-Pereira, Ana Rodríguez Bernaldo de Quirós, Raquel Sendón. Presence of Perfluoroalkyl and Polyfluoroalkyl Substances (PFAS) in Food Contact Materials (FCM) and Its Migration to Food. Foods. 2021; 10 (7):1443.
Chicago/Turabian StyleArabela Ramírez Carnero; Antía Lestido-Cardama; Patricia Vazquez Loureiro; Letricia Barbosa-Pereira; Ana Rodríguez Bernaldo de Quirós; Raquel Sendón. 2021. "Presence of Perfluoroalkyl and Polyfluoroalkyl Substances (PFAS) in Food Contact Materials (FCM) and Its Migration to Food." Foods 10, no. 7: 1443.
Cocoa bean shell is one of the main by-products of chocolate manufacturing and possesses several compounds with biofunctionalities. It can function as an antibacterial agent, and its action is mostly reported against Streptococcus mutans. However, only a few studies have investigated the cocoa bean shell compounds responsible for this activity. This study aimed to evaluate several extracts of cocoa bean shells from different geographical origins and cocoa varieties and estimate their antimicrobial properties against different fungal and bacterial strains by determining their minimal inhibitory concentration. The results demonstrated antimicrobial activity of cocoa bean shell against one of the tested strains, S. mutans. Cocoa bean shell extracts were further analysed via LC-HRMS for untargeted metabolomic analysis. LC-HRMS data were analysed (preprocessing and statistical analyses) using the Workflow4Metabolomics platform. The latter enabled us to identify possible compounds responsible for the detected antimicrobial activity by comparing the more and less active extracts. Active extracts were not the most abundant in polyphenols but contained higher concentrations of two metabolites. After tentative annotation of these metabolites, one of them was identified and confirmed to be 7-methylxanthine. When tested alone, 7-methylxanthine did not display antibacterial activity. However, a possible cocktail effect due to the synergistic activity of this molecule along with other compounds in the cocoa bean shell extracts cannot be neglected. In conclusion, cocoa bean shell could be a functional ingredient with benefits for human health as it exhibited antibacterial activity against S. mutans. However, the antimicrobial mechanisms still need to be confirmed.
Olga Rojo-Poveda; Sofia Oliveira Ribeiro; Cèlia Anton-Sales; Flore Keymeulen; Letricia Barbosa-Pereira; Cédric Delporte; Giuseppe Zeppa; Caroline Stévigny. Evaluation of Cocoa Bean Shell Antimicrobial Activity: A Tentative Assay Using a Metabolomic Approach for Active Compound Identification. Planta Medica 2021, 1 .
AMA StyleOlga Rojo-Poveda, Sofia Oliveira Ribeiro, Cèlia Anton-Sales, Flore Keymeulen, Letricia Barbosa-Pereira, Cédric Delporte, Giuseppe Zeppa, Caroline Stévigny. Evaluation of Cocoa Bean Shell Antimicrobial Activity: A Tentative Assay Using a Metabolomic Approach for Active Compound Identification. Planta Medica. 2021; ():1.
Chicago/Turabian StyleOlga Rojo-Poveda; Sofia Oliveira Ribeiro; Cèlia Anton-Sales; Flore Keymeulen; Letricia Barbosa-Pereira; Cédric Delporte; Giuseppe Zeppa; Caroline Stévigny. 2021. "Evaluation of Cocoa Bean Shell Antimicrobial Activity: A Tentative Assay Using a Metabolomic Approach for Active Compound Identification." Planta Medica , no. : 1.
Metabolomics applications to the aquaculture research are increasing steadily. The use of standardized proton nuclear magnetic resonance (1H NMR) spectroscopy can provide the aquaculture industry with an unbiased, reproducible, and high-throughput screening tool, which can help to diagnose nutritional and disease-related metabolic disorders in farmed fish. Standard operating procedures developed for analysing (human) plasma by 1H NMR were applied to fingerprint the metabolome in plasma samples collected from Atlantic salmon. The aim was to explore the metabolome of salmon plasma in relation to growth stage and sampling site. A total of 72 salmon were collected from three aquaculture sites in Norway (Lat. 65, 67, and 70 °N) and over two sampling events (December 2017 and November 2018). Plasma drawn from each salmon was measured by 1H NMR and metabolites were quantified using the SigMa software. The NMR data was analysed by principal component analysis (PCA) and ANOVA-simultaneous component analysis (ASCA). Important metabolic differences were evidenced, with adult salmon having a much higher content of very low-density lipoproteins and cholesterol in their plasma, while smolts displayed significantly higher levels of propylene glycol. Overall, 24% of the metabolite variation was due to the growth stage, whereas 12% of the metabolite variation was related to the aquaculture site and practice (p < 0.001). This study provides a baseline investigation of the plasma metabolome of the Atlantic salmon and demonstrates how 1H NMR metabolomics can be used in future investigations for comparing aquaculture practices and their influence on the fish metabolome.
Violetta Aru; Bekzod Khakimov; Klavs Martin Sørensen; Elvis Mashingaidze Chikwati; Trond M. Kortner; Paul Midtlyng; Åshild Krogdahl; Søren Balling Engelsen. The plasma metabolome of Atlantic salmon as studied by 1H NMR spectroscopy using standard operating procedures: effect of aquaculture location and growth stage. Metabolomics 2021, 17, 1 -13.
AMA StyleVioletta Aru, Bekzod Khakimov, Klavs Martin Sørensen, Elvis Mashingaidze Chikwati, Trond M. Kortner, Paul Midtlyng, Åshild Krogdahl, Søren Balling Engelsen. The plasma metabolome of Atlantic salmon as studied by 1H NMR spectroscopy using standard operating procedures: effect of aquaculture location and growth stage. Metabolomics. 2021; 17 (6):1-13.
Chicago/Turabian StyleVioletta Aru; Bekzod Khakimov; Klavs Martin Sørensen; Elvis Mashingaidze Chikwati; Trond M. Kortner; Paul Midtlyng; Åshild Krogdahl; Søren Balling Engelsen. 2021. "The plasma metabolome of Atlantic salmon as studied by 1H NMR spectroscopy using standard operating procedures: effect of aquaculture location and growth stage." Metabolomics 17, no. 6: 1-13.
Necrotising enterocolitis (NEC) is a microbiome-dependent gut disease in preterm infants in early life. Antibiotic treatment is a common intervention for NEC. How NEC lesions, with or without antibiotics, affect plasma metabolome was explored in this study. Formula-fed preterm pigs were used as a model for human NEC and treated with saline, parenteral or oral antibiotics (n = 15–17) for four days after delivery. Gut tissues were collected for evaluation of NEC-like lesions and plasma for metabolomic analysis by proton nuclear magnetic resonance spectroscopy (1H-NMR). Metabolites were annotated, quantified and subjected to statistical modelling to delineate the effects of NEC and antibiotic treatment. Presence of severe NEC lesions, not antibiotic treatment, was the main drive for plasma metabolite changes. Relative to other pigs, pigs with severe NEC lesions had higher levels of alanine, histidine and myo-inositol, and lower levels of 3-hydroxybutyric acid and isobutyric acid. Across NEC lesion states (healthy, mild, severe), antibiotics directly affected only a few metabolites (tryptophan, 3-phenyllactic acid). Together and independently, NEC and antibiotic treatment affected circulating metabolites in preterm pigs. Amino acids and plasma metabolites, partly related to the gut microbiome, may be helpful to monitor progression of NEC lesions after proper validation.
Yan-Nan Jiang; Yong-Xin Ye; Per Sangild; Thomas Thymann; Søren Engelsen; Bekzod Khakimov; Ping-Ping Jiang. Plasma Metabolomics to Evaluate Progression of Necrotising Enterocolitis in Preterm Pigs. Metabolites 2021, 11, 283 .
AMA StyleYan-Nan Jiang, Yong-Xin Ye, Per Sangild, Thomas Thymann, Søren Engelsen, Bekzod Khakimov, Ping-Ping Jiang. Plasma Metabolomics to Evaluate Progression of Necrotising Enterocolitis in Preterm Pigs. Metabolites. 2021; 11 (5):283.
Chicago/Turabian StyleYan-Nan Jiang; Yong-Xin Ye; Per Sangild; Thomas Thymann; Søren Engelsen; Bekzod Khakimov; Ping-Ping Jiang. 2021. "Plasma Metabolomics to Evaluate Progression of Necrotising Enterocolitis in Preterm Pigs." Metabolites 11, no. 5: 283.
In analytical chemistry, multivariate calibration is applied when substituting a time-consuming reference measurement (based on e.g. chromatography) with a high-throughput measurement (based on e.g. vibrational spectroscopy). An average error term, of the response variable, is often used to evaluate the performance of a calibration model. However, indirect relationships, between the response and explanatory variables, may be used for calibration. In such cases, model validity cannot necessarily be determined solely by the average error term. One should also consider the use of the models, as well as the validity of the indirect relationships in future samples. If the analyte of interest is partly quantified from signals of interfering compounds, then these interfering compounds will play a hidden role in the calibration. This hidden role may affect future use of the calibration model as strong covariance relationships between analyte estimates and interfering compounds may be imposed. Hence, such model cannot detect changes in the relationship between the analyte and interfering compounds. The problem is called the cage of covariance. This paper discusses the concept cage of covariance and possible consequences of applying models exposed to this issue.
Carl Emil Eskildsen; Tormod Næs; Peter B. Skou; Lars Erik Solberg; Katinka R. Dankel; Silje A. Basmoen; Jens Petter Wold; Siri S. Horn; Borghild Hillestad; Nina A. Poulsen; Mette Christensen; Theo Pieper; Nils Kristian Afseth; Søren B. Engelsen. Cage of covariance in calibration modeling: Regressing multiple and strongly correlated response variables onto a low rank subspace of explanatory variables. Chemometrics and Intelligent Laboratory Systems 2021, 213, 104311 .
AMA StyleCarl Emil Eskildsen, Tormod Næs, Peter B. Skou, Lars Erik Solberg, Katinka R. Dankel, Silje A. Basmoen, Jens Petter Wold, Siri S. Horn, Borghild Hillestad, Nina A. Poulsen, Mette Christensen, Theo Pieper, Nils Kristian Afseth, Søren B. Engelsen. Cage of covariance in calibration modeling: Regressing multiple and strongly correlated response variables onto a low rank subspace of explanatory variables. Chemometrics and Intelligent Laboratory Systems. 2021; 213 ():104311.
Chicago/Turabian StyleCarl Emil Eskildsen; Tormod Næs; Peter B. Skou; Lars Erik Solberg; Katinka R. Dankel; Silje A. Basmoen; Jens Petter Wold; Siri S. Horn; Borghild Hillestad; Nina A. Poulsen; Mette Christensen; Theo Pieper; Nils Kristian Afseth; Søren B. Engelsen. 2021. "Cage of covariance in calibration modeling: Regressing multiple and strongly correlated response variables onto a low rank subspace of explanatory variables." Chemometrics and Intelligent Laboratory Systems 213, no. : 104311.
Inhaled administration of ethanol in the early stages of COVID-19 would favor its location on the initial replication sites, being able to reduce the progression of the disease and improving its prognosis. Before evaluating the efficacy and safety of this novel therapeutic strategy in humans, its characterization is required. The developed 65° ethanol formulation is stable at room temperature and protected from light for 15 days, maintaining its physicochemical and microbiological properties. Two oxygen flows have been tested for its administration (2 and 3 L/min) using an automated headspace gas chromatographic analysis technique (HS-GC-MS), with that of 2 L/min being the most appropriate one, ensuring the inhalation of an ethanol daily dose of 33.6 ± 3.6 mg/min and achieving more stable concentrations during the entire treatment (45 min). Under these conditions of administration, the formulation has proven to be safe, based on histological studies of the respiratory tracts and lungs of rats. On the other hand, these results are accompanied by the first preclinical molecular imaging study with radiolabeled ethanol administered by this route. The current ethanol formulation has received approval from the Spanish Agency of Medicines and Medical Devices for a phase II clinical trial for early-stage COVID-19 patients, which is currently in the recruitment phase (ALCOVID-19; EudraCT number: 2020-001760-29).
Ana Castro-Balado; Cristina Mondelo-García; Letricia Barbosa-Pereira; Iria Varela-Rey; Ignacio Novo-Veleiro; Néstor Vázquez-Agra; José Antúnez-López; Enrique Bandín-Vilar; Raquel Sendón-García; Manuel Busto-Iglesias; Ana Rodríguez-Bernaldo de Quirós; Laura García-Quintanilla; Miguel González-Barcia; Irene Zarra-Ferro; Francisco Otero-Espinar; David Rey-Bretal; José Lago-Quinteiro; Luis Valdés-Cuadrado; Carlos Rábade-Castedo; María del Río-Garma; Carlos Crespo-Diz; Olga Delgado-Sánchez; Pablo Aguiar; Gema Barbeito-Castiñeiras; María Pérez del Molino-Bernal; Rocío Trastoy-Pena; Rossana Passannante; Jordi Llop; Antonio Pose-Reino; Anxo Fernández-Ferreiro. Development and Characterization of Inhaled Ethanol as a Novel Pharmacological Strategy Currently Evaluated in a Phase II Clinical Trial for Early-Stage SARS-CoV-2 Infection. Pharmaceutics 2021, 13, 342 .
AMA StyleAna Castro-Balado, Cristina Mondelo-García, Letricia Barbosa-Pereira, Iria Varela-Rey, Ignacio Novo-Veleiro, Néstor Vázquez-Agra, José Antúnez-López, Enrique Bandín-Vilar, Raquel Sendón-García, Manuel Busto-Iglesias, Ana Rodríguez-Bernaldo de Quirós, Laura García-Quintanilla, Miguel González-Barcia, Irene Zarra-Ferro, Francisco Otero-Espinar, David Rey-Bretal, José Lago-Quinteiro, Luis Valdés-Cuadrado, Carlos Rábade-Castedo, María del Río-Garma, Carlos Crespo-Diz, Olga Delgado-Sánchez, Pablo Aguiar, Gema Barbeito-Castiñeiras, María Pérez del Molino-Bernal, Rocío Trastoy-Pena, Rossana Passannante, Jordi Llop, Antonio Pose-Reino, Anxo Fernández-Ferreiro. Development and Characterization of Inhaled Ethanol as a Novel Pharmacological Strategy Currently Evaluated in a Phase II Clinical Trial for Early-Stage SARS-CoV-2 Infection. Pharmaceutics. 2021; 13 (3):342.
Chicago/Turabian StyleAna Castro-Balado; Cristina Mondelo-García; Letricia Barbosa-Pereira; Iria Varela-Rey; Ignacio Novo-Veleiro; Néstor Vázquez-Agra; José Antúnez-López; Enrique Bandín-Vilar; Raquel Sendón-García; Manuel Busto-Iglesias; Ana Rodríguez-Bernaldo de Quirós; Laura García-Quintanilla; Miguel González-Barcia; Irene Zarra-Ferro; Francisco Otero-Espinar; David Rey-Bretal; José Lago-Quinteiro; Luis Valdés-Cuadrado; Carlos Rábade-Castedo; María del Río-Garma; Carlos Crespo-Diz; Olga Delgado-Sánchez; Pablo Aguiar; Gema Barbeito-Castiñeiras; María Pérez del Molino-Bernal; Rocío Trastoy-Pena; Rossana Passannante; Jordi Llop; Antonio Pose-Reino; Anxo Fernández-Ferreiro. 2021. "Development and Characterization of Inhaled Ethanol as a Novel Pharmacological Strategy Currently Evaluated in a Phase II Clinical Trial for Early-Stage SARS-CoV-2 Infection." Pharmaceutics 13, no. 3: 342.
Lipoprotein subfractions are biomarkers for early diagnosis of cardiovascular diseases. The reference method, ultracentrifugation, for measuring lipoproteins is time consuming and there is a need to develop a rapid method for cohort screenings. Here we present partial least squares regression models developed using 1H-NMR spectra and concentrations of lipoproteins as measured by ultracentrifugation on 316 healthy Danes. Different regions of the 1H-NMR spectra representing signals of the lipoproteins and different lipid species were investigated to develop parsimonious, reliable and best performing prediction models. 65 LP main and subfractions were predictable with an accuracy Q2 of > 0.6 on test set samples. The models were tested on an independent cohort of 290 healthy Swedes with predicted and reference values matching by up to 85-95%. The software was developed to predict lipoproteins in human blood using 1H-NMR spectra and made freely available to be applied for future cohort screenings.
Bekzod Khakimov; Huub C.J. Hoefsloot; Nabiollah Mobaraki; Violetta Aru; Mette Kristensen; Mads V. Lind; Lars Holm; Josué L Castro-Mejía; Dennis S Nielsen; Doris M. Jacobs; Age K. Smilde; Søren Balling Engelsen. Human blood lipoprotein predictions from 1H NMR spectra: protocol, model performances and cage of covariance. 2021, 1 .
AMA StyleBekzod Khakimov, Huub C.J. Hoefsloot, Nabiollah Mobaraki, Violetta Aru, Mette Kristensen, Mads V. Lind, Lars Holm, Josué L Castro-Mejía, Dennis S Nielsen, Doris M. Jacobs, Age K. Smilde, Søren Balling Engelsen. Human blood lipoprotein predictions from 1H NMR spectra: protocol, model performances and cage of covariance. . 2021; ():1.
Chicago/Turabian StyleBekzod Khakimov; Huub C.J. Hoefsloot; Nabiollah Mobaraki; Violetta Aru; Mette Kristensen; Mads V. Lind; Lars Holm; Josué L Castro-Mejía; Dennis S Nielsen; Doris M. Jacobs; Age K. Smilde; Søren Balling Engelsen. 2021. "Human blood lipoprotein predictions from 1H NMR spectra: protocol, model performances and cage of covariance." , no. : 1.
Background: Cocoa bean shell (CBS), a main byproduct of cocoa processing, represents a source of components such as polyphenols and methylxanthines, which have been associated with a reduced risk of several diseases. Therefore, CBS has potential application as a food ingredient. Intestinal mucosa is exposed to immune and inflammatory responses triggered by dietary agents, such as oxysterols, which derive from cholesterol oxidation and are pro-oxidant compounds able to affect intestinal function. We aimed at investigating the capability of the Forastero cultivar CBS, added or not added to ice cream, to protect against the intestinal barrier damage induced by a dietary oxysterol mixture. Methods: Composition and antioxidant capacity of in vitro digested CBS and CBS-enriched ice cream were analyzed by high-performance liquid chromatography and 1,1-diphenyl-2-picryl-hydrazyl radical-scavenging assay, respectively. CaCo-2 cells differentiated into enterocyte-like monolayer were incubated with 60 µM oxysterol mixture in the presence of CBS formulations. Results: The oxysterol mixture induced tight junction impairment, interleukin-8 and monocyte chemoattractant protein-1 cell release, and oxidative stress-related nuclear factor erythroid 2 p45-related factor 2 response Nrf2. Both CBSs protected cells from these adverse effects, probably thanks to their high phenolic content. CBS-enriched ice cream showed the highest antioxidant capacity. Theobromine, which is in high concentrations of CBS, was also tested. Although theobromine exerted no effect on Nrf2 expression, its anti-inflammatory cooperating activity in CBS effect cannot be excluded. Conclusions: Our findings suggest that CBS-enriched ice cream may be effective in the prevention of gut integrity damage associated with oxidative/inflammatory reactions.
Daniela Rossin; Letricia Barbosa-Pereira; Noemi Iaia; Barbara Sottero; Alice Danzero; Giuseppe Poli; Giuseppe Zeppa; Fiorella Biasi. Protective Effect of Cocoa Bean Shell against Intestinal Damage: An Example of Byproduct Valorization. Antioxidants 2021, 10, 280 .
AMA StyleDaniela Rossin, Letricia Barbosa-Pereira, Noemi Iaia, Barbara Sottero, Alice Danzero, Giuseppe Poli, Giuseppe Zeppa, Fiorella Biasi. Protective Effect of Cocoa Bean Shell against Intestinal Damage: An Example of Byproduct Valorization. Antioxidants. 2021; 10 (2):280.
Chicago/Turabian StyleDaniela Rossin; Letricia Barbosa-Pereira; Noemi Iaia; Barbara Sottero; Alice Danzero; Giuseppe Poli; Giuseppe Zeppa; Fiorella Biasi. 2021. "Protective Effect of Cocoa Bean Shell against Intestinal Damage: An Example of Byproduct Valorization." Antioxidants 10, no. 2: 280.
Fruit by-products have a low economic value and have proven biological activities, such as antioxidant capacity due to the presence of active compounds. The main objective of this study was to obtain and determine the antioxidant capacity, through DPPH radical assay and β-carotene bleaching assay, of three food grade extracts from apple, lemon, and orange industrial by-products. Furthermore, the extracts were characterized by ultra-high performance liquid chromatography coupled to mass spectrometry (UHPLC-MS/MS). LC with diode array detector (LC-DAD) was used for the quantification of the main polyphenols. Lemon extract presented the highest inhibition percentage of DPPH radical (51.7%) and the highest total phenolics content (43.4 mg GAE/g) from the by-products studied. Orange by-product was that with the higher number of polyphenols while lemon extract was that with the highest content of individual phenolics. The by-product obtained from the lemon was that with higher amounts of hydroxycinnamic acids (407 µg/g of by-product), mainly chlorogenic acid (386.7 µg/g), followed by the apple by-product (128.0 µg/g of by-product), which showed higher amounts of rosmarinic and chlorogenic acids. These industrial by-products have great potential as a source of natural antioxidants to be used directly as food additives or to be incorporated in packaging to produce active food packaging.
Cássia Barbosa; Mariana Andrade; Raquel Séndon; Ana Silva; Fernando Ramos; Fernanda Vilarinho; Khaoula Khwaldia; Letricia Barbosa-Pereira. Industrial Fruits By-Products and Their Antioxidant Profile: Can They Be Exploited for Industrial Food Applications? Foods 2021, 10, 272 .
AMA StyleCássia Barbosa, Mariana Andrade, Raquel Séndon, Ana Silva, Fernando Ramos, Fernanda Vilarinho, Khaoula Khwaldia, Letricia Barbosa-Pereira. Industrial Fruits By-Products and Their Antioxidant Profile: Can They Be Exploited for Industrial Food Applications? Foods. 2021; 10 (2):272.
Chicago/Turabian StyleCássia Barbosa; Mariana Andrade; Raquel Séndon; Ana Silva; Fernando Ramos; Fernanda Vilarinho; Khaoula Khwaldia; Letricia Barbosa-Pereira. 2021. "Industrial Fruits By-Products and Their Antioxidant Profile: Can They Be Exploited for Industrial Food Applications?" Foods 10, no. 2: 272.
Forward-focused molecular genetics is successfully framing DNA diversity and mapping primary gene functions. However, abandoning the classic Linnaean fingerprint link between the phenome and genome by suppressing gene interaction (pleiotropy), has resulted in a genome-to-phenome gap and poor utilization of molecular data. We demonstrate how to bridge this gap by using an example of a barley mutant seed model, where pleiotropy is observed as covarying global molecular patterns that define each endosperm. Global coherence was discovered as a covariate coordinator within and between local genotype specific fingerprints. This implies that any of these fingerprints can select its recombinant global phenotype variant, including composition. Introducing the law of coherence, and the movement of gene complexes by chemical fingerprint traits as selectors, introduces a revolution in understanding physiological molecular genetics and plant-breeding.
Lars Munck; Åsmund Rinnan; Bekzod Khakimov; Birthe Møller Jespersen; Søren Balling Engelsen. Physiological Genetics Reformed: Bridging the Genome-to-Phenome Gap by Coherent Chemical Fingerprints – the Global Coordinator. Trends in Plant Science 2021, 26, 324 -337.
AMA StyleLars Munck, Åsmund Rinnan, Bekzod Khakimov, Birthe Møller Jespersen, Søren Balling Engelsen. Physiological Genetics Reformed: Bridging the Genome-to-Phenome Gap by Coherent Chemical Fingerprints – the Global Coordinator. Trends in Plant Science. 2021; 26 (4):324-337.
Chicago/Turabian StyleLars Munck; Åsmund Rinnan; Bekzod Khakimov; Birthe Møller Jespersen; Søren Balling Engelsen. 2021. "Physiological Genetics Reformed: Bridging the Genome-to-Phenome Gap by Coherent Chemical Fingerprints – the Global Coordinator." Trends in Plant Science 26, no. 4: 324-337.
Miguel De León-Zapata; Lorenzo Pastrana-Castro; Letricia Barbosa-Pereira; María L. Rua-Rodríguez; Janeth Ventura; Thalia Salinas; Raul Rodríguez; Juan A. Ascacio-Valdés; José Sandoval-Cortés; Cristóbal N. Aguilar. Nanoemulsions for Edible Coatings: Stabilizing and Bioactive Properties. Practical Applications of Physical Chemistry in Food Science and Technology 2020, 183 -198.
AMA StyleMiguel De León-Zapata, Lorenzo Pastrana-Castro, Letricia Barbosa-Pereira, María L. Rua-Rodríguez, Janeth Ventura, Thalia Salinas, Raul Rodríguez, Juan A. Ascacio-Valdés, José Sandoval-Cortés, Cristóbal N. Aguilar. Nanoemulsions for Edible Coatings: Stabilizing and Bioactive Properties. Practical Applications of Physical Chemistry in Food Science and Technology. 2020; ():183-198.
Chicago/Turabian StyleMiguel De León-Zapata; Lorenzo Pastrana-Castro; Letricia Barbosa-Pereira; María L. Rua-Rodríguez; Janeth Ventura; Thalia Salinas; Raul Rodríguez; Juan A. Ascacio-Valdés; José Sandoval-Cortés; Cristóbal N. Aguilar. 2020. "Nanoemulsions for Edible Coatings: Stabilizing and Bioactive Properties." Practical Applications of Physical Chemistry in Food Science and Technology , no. : 183-198.
Cocoa bean shell (CBS), a by-product with considerable concentrations of bioactive compounds and proven biofunctional potential, has been demonstrated to be a suitable ingredient for high-fiber functional biscuits adapted to diabetic consumers. In this work, the in vitro bioaccessibility and intestinal absorption of polyphenols and methylxanthines contained in these biscuits were evaluated, and the effect of the food matrix was studied. Biscuits containing CBS and the CBS alone underwent in vitro digestion followed by an intestinal permeability study. The results confirmed that compounds were less bioavailable in the presence of a food matrix, although the digestion contributed to their release from this matrix, increasing the concentrations available at the intestinal level and making them capable of promoting antioxidant and antidiabetic activities. After digestion, CBS biscuits were shown to possess α-glucosidase inhibition capacity comparable to that of acarbose. Moreover, the presence of the food matrix improved the stability of polyphenols throughout the digestion process. Intestinal absorption of flavan-3-ols seemed to be limited to a maximum threshold and was therefore independent of the sample, while procyanidin was not absorbed. Methylxanthine absorption was high and was boosted by the presence of the food matrix. The results confirmed the biofunctional potential of CBS-based biscuits.
Olga Rojo-Poveda; Letricia Barbosa-Pereira; Charaf El Khattabi; Estelle N.H. Youl; Marta Bertolino; Cédric Delporte; Stéphanie Pochet; Caroline Stévigny. Polyphenolic and Methylxanthine Bioaccessibility of Cocoa Bean Shell Functional Biscuits: Metabolomics Approach and Intestinal Permeability through Caco-2 Cell Models. Antioxidants 2020, 9, 1164 .
AMA StyleOlga Rojo-Poveda, Letricia Barbosa-Pereira, Charaf El Khattabi, Estelle N.H. Youl, Marta Bertolino, Cédric Delporte, Stéphanie Pochet, Caroline Stévigny. Polyphenolic and Methylxanthine Bioaccessibility of Cocoa Bean Shell Functional Biscuits: Metabolomics Approach and Intestinal Permeability through Caco-2 Cell Models. Antioxidants. 2020; 9 (11):1164.
Chicago/Turabian StyleOlga Rojo-Poveda; Letricia Barbosa-Pereira; Charaf El Khattabi; Estelle N.H. Youl; Marta Bertolino; Cédric Delporte; Stéphanie Pochet; Caroline Stévigny. 2020. "Polyphenolic and Methylxanthine Bioaccessibility of Cocoa Bean Shell Functional Biscuits: Metabolomics Approach and Intestinal Permeability through Caco-2 Cell Models." Antioxidants 9, no. 11: 1164.
Melatonin is a hormone produced by the pineal gland and extra-pineal tissues with chronobiotic effects that can alter the circadian rhythms, among other health benefits. This study intended to compare two different methods, high-performance liquid chromatography coupled to a variable wavelength detector plus a fluorescence detector (HPLC-VWD-FLD) and constant-wavelength synchronous spectrofluorimetry, to determine melatonin in food supplements. HPLC-MS/MS was used as a confirmatory technique. The data showed that both analytical methodologies were valid for the determination of melatonin with correlation between the datasets obtained (99% confidence level). Constant-wavelength synchronous spectrofluorimetry is a fast, simple, and economical method that can be used for a rapid and precise determination of melatonin in supplements, in the absence of other compounds that could interfere in the analysis. On the other hand, the HPLC-VWD-FLD method showed to be more suitable for the determination of melatonin in complex samples because of its high sensitivity and separation efficiency. Finally, all supplements analyzed from the Spanish market comply with the melatonin content declared on the label and with the claim “contributes to the alleviation of subjective feelings of the jet lag of jet lag” (0.5 mg/day). Also, 7 out of 10 complain about the claim “contributes to the reduction of time taken to fall asleep” (1 mg/day).
Sandra Barreiro Astray; Letricia Barbosa-Pereira; María Asunción Lage-Yusty; Julia López-Hernández. Comparison of Analytical Methods for the Rapid Determination of Melatonin in Food Supplements. Food Analytical Methods 2020, 14, 734 -741.
AMA StyleSandra Barreiro Astray, Letricia Barbosa-Pereira, María Asunción Lage-Yusty, Julia López-Hernández. Comparison of Analytical Methods for the Rapid Determination of Melatonin in Food Supplements. Food Analytical Methods. 2020; 14 (4):734-741.
Chicago/Turabian StyleSandra Barreiro Astray; Letricia Barbosa-Pereira; María Asunción Lage-Yusty; Julia López-Hernández. 2020. "Comparison of Analytical Methods for the Rapid Determination of Melatonin in Food Supplements." Food Analytical Methods 14, no. 4: 734-741.
This chapter is a primer on the use of multivariate data analysis—or chemometrics—to near-infrared spectra. The extraordinary synergy between near-infrared spectroscopy and the data analysis methods called chemometrics has led to a green analytical revolution in practically all areas of life sciences and related industries for quality control and process monitoring. The near-infrared spectroscopy method is nondestructive, rapid and environmentally friendly. However, the most unique advantage of near-infrared spectroscopy is that it can measure samples remotely and unbiased, as is, i.e., solids and liquids without interfering with the sample or sample preparation. The success of near-infrared spectroscopy would not have been possible without the chemometric data processing. This chapter gives an overview, including tricks of the trade, of the most common chemometric techniques for analysis of near-infrared spectral ensembles illustrated by downloadable data examples.
Klavs Martin Sørensen; Frans Van Den Berg; Søren Balling Engelsen. NIR Data Exploration and Regression by Chemometrics—A Primer. Near-Infrared Spectroscopy 2020, 127 -189.
AMA StyleKlavs Martin Sørensen, Frans Van Den Berg, Søren Balling Engelsen. NIR Data Exploration and Regression by Chemometrics—A Primer. Near-Infrared Spectroscopy. 2020; ():127-189.
Chicago/Turabian StyleKlavs Martin Sørensen; Frans Van Den Berg; Søren Balling Engelsen. 2020. "NIR Data Exploration and Regression by Chemometrics—A Primer." Near-Infrared Spectroscopy , no. : 127-189.
The bioconversion of fruit by-products into new functional and clean label ingredients/additives represents a sustainable approach with great potential for application in the food sector
Letricia Barbosa-Pereira; Mariana A. Andrade; Cássia H. Barbosa; Fernanda Vilarinho; Ana Sanches-Silva; Ana Rodriguez-Bernaldo de Quirós; Raquel Sendón. Phenolic Profile of Fruit Industry Byproducts Determined by LC–DAD–MS/MS. Proceedings 2020, 70, 31 .
AMA StyleLetricia Barbosa-Pereira, Mariana A. Andrade, Cássia H. Barbosa, Fernanda Vilarinho, Ana Sanches-Silva, Ana Rodriguez-Bernaldo de Quirós, Raquel Sendón. Phenolic Profile of Fruit Industry Byproducts Determined by LC–DAD–MS/MS. Proceedings. 2020; 70 (1):31.
Chicago/Turabian StyleLetricia Barbosa-Pereira; Mariana A. Andrade; Cássia H. Barbosa; Fernanda Vilarinho; Ana Sanches-Silva; Ana Rodriguez-Bernaldo de Quirós; Raquel Sendón. 2020. "Phenolic Profile of Fruit Industry Byproducts Determined by LC–DAD–MS/MS." Proceedings 70, no. 1: 31.
A great number of factors can influence milk fermentation for yoghurt production such as fermentation conditions, starter cultures and milk characteristics. It is important for dairy companies to know the best combinations of these parameters for a controlled fermentation and for the desired qualities of yoghurt. This study investigates the use of a 1H-NMR metabolomics approach to monitor the changes in milk during fermentation from time 0 to 24 h, taking samples every hour in the first 8 h and then at the end-point at 24 h. Three different starter cultures (L. delbrueckii ssp. bulgaricus, S. thermophilus and their combination) were used and two different heat treatments (99 or 105 °C) were applied to milk. The results clearly show the breakdown of proteins and lactose as well as the concomitant increase in acetate, lactate and citrate during fermentation. Formate is found at different initial concentrations depending on the heat treatment of the milk and its different time trajectory depends on the starter cultures: Lactobacillus cannot produce formate, but needs it for growth, whilst Streptococcus is able to produce formate from pyruvate, therefore promoting the symbiotic relationship between the two strains. On the other hand, Lactobacillus can hydrolyze milk proteins into amino acids, enriching the quality of the final product. In this way, better insight into the protocooperation of lactic acid bacteria strains and information on the impact of a greater heat treatment in the initial matrix were obtained. The global chemical view on the fermentations provided using NMR is key information for yoghurt producers and companies producing starter cultures.
Alessia Trimigno; Christian Bøge Lyndgaard; Guðrún Anna Atladóttir; Violetta Aru; Søren Balling Engelsen; Line Clemmensen. An NMR Metabolomics Approach to Investigate Factors Affecting the Yoghurt Fermentation Process and Quality. Metabolites 2020, 10, 293 .
AMA StyleAlessia Trimigno, Christian Bøge Lyndgaard, Guðrún Anna Atladóttir, Violetta Aru, Søren Balling Engelsen, Line Clemmensen. An NMR Metabolomics Approach to Investigate Factors Affecting the Yoghurt Fermentation Process and Quality. Metabolites. 2020; 10 (7):293.
Chicago/Turabian StyleAlessia Trimigno; Christian Bøge Lyndgaard; Guðrún Anna Atladóttir; Violetta Aru; Søren Balling Engelsen; Line Clemmensen. 2020. "An NMR Metabolomics Approach to Investigate Factors Affecting the Yoghurt Fermentation Process and Quality." Metabolites 10, no. 7: 293.
The cocoa bean shell (CBS), a by-product of the cocoa industry, has been reported to be rich in fiber and polyphenols, which could contribute to reducing the metabolism of sugars and glucose adsorption. The production of CBS-based biscuits in which sucrose is replaced with tagatose (a low-glycemic sugar with prebiotic properties), benefiting diabetic consumers, is proposed. Six prototype biscuits were produced using sucrose, tagatose, and CBS powder at 0%, 10%, and 20% as a wheat flour replacement. Biscuits were studied in terms of fiber content, and those with 10% and 20% CBS showed to contain 5.66% and 8.70–8.71% of total dietary fiber, respectively. Moreover, the physicochemical and structural properties of the biscuits were studied to evaluate their differences due to the use of sucrose and tagatose combined with CBS. Significant effects mainly caused by the reducing nature and lower solubility of tagatose with respect to sugar, and the water retention capacities of CBS were observed. Finally, the biscuits were evaluated by performing a consumer acceptance evaluation, and their perceptible sensorial differences were studied by performing a Napping® sensory characterization. CBS-based biscuits represent an interesting possibility for cocoa by-product revalorization, although an optimized recipe is recommended, especially when employing tagatose.
Olga Rojo-Poveda; Letricia Barbosa-Pereira; David Orden; Caroline Stévigny; Giuseppe Zeppa; Marta Bertolino. Physical Properties and Consumer Evaluation of Cocoa Bean Shell-Functionalized Biscuits Adapted for Diabetic Consumers by the Replacement of Sucrose with Tagatose. Foods 2020, 9, 814 .
AMA StyleOlga Rojo-Poveda, Letricia Barbosa-Pereira, David Orden, Caroline Stévigny, Giuseppe Zeppa, Marta Bertolino. Physical Properties and Consumer Evaluation of Cocoa Bean Shell-Functionalized Biscuits Adapted for Diabetic Consumers by the Replacement of Sucrose with Tagatose. Foods. 2020; 9 (6):814.
Chicago/Turabian StyleOlga Rojo-Poveda; Letricia Barbosa-Pereira; David Orden; Caroline Stévigny; Giuseppe Zeppa; Marta Bertolino. 2020. "Physical Properties and Consumer Evaluation of Cocoa Bean Shell-Functionalized Biscuits Adapted for Diabetic Consumers by the Replacement of Sucrose with Tagatose." Foods 9, no. 6: 814.
The cocoa bean shell (CBS), a cocoa by-product, contains a significant number of bioactive compounds with functional properties, such as polyphenols and methylxanthines, and is used as an ingredient in beverages and foods. In this work, the bioaccessibility of polyphenols and methylxanthines after In Vitro digestion was evaluated in new flavoured beverages for at-home consumption (capsules and tea bags). In addition, the polyphenolic composition, functional properties (antiradical and α-glucosidase inhibition capacities) and consumer acceptability of these beverages were evaluated. In both capsule and tea bag beverages, the bioaccessibility of methylxanthines was 100% while that of total polyphenols exceeded 50%. The main polyphenols determined using reverse-phase liquid chromatography were type B procyanidins and epicatechin. The antiradical activity in capsule and tea bag beverages was 1.75 and 1.88 mM of Trolox equivalents, respectively, of which 59.50% and 57.09% were recovered after simulated digestion. The percentage of α-glucosidase inhibition before In Vitro digestion (51.64% and 53.82% for capsules and tea bags, respectively) was comparable to that of acarbose at 0.5 mM. All the beverages obtained a high consumer acceptability. Therefore, these results highlight that CBSs can be used as a valid source of bioactive compounds in the preparation of beverages with homemade techniques.
Carolina Cantele; Olga Rojo-Poveda; Marta Bertolino; Daniela Ghirardello; Vladimiro Cardenia; Letricia Barbosa-Pereira; Giuseppe Zeppa. In Vitro Bioaccessibility and Functional Properties of Phenolic Compounds from Enriched Beverages Based on Cocoa Bean Shell. Foods 2020, 9, 715 .
AMA StyleCarolina Cantele, Olga Rojo-Poveda, Marta Bertolino, Daniela Ghirardello, Vladimiro Cardenia, Letricia Barbosa-Pereira, Giuseppe Zeppa. In Vitro Bioaccessibility and Functional Properties of Phenolic Compounds from Enriched Beverages Based on Cocoa Bean Shell. Foods. 2020; 9 (6):715.
Chicago/Turabian StyleCarolina Cantele; Olga Rojo-Poveda; Marta Bertolino; Daniela Ghirardello; Vladimiro Cardenia; Letricia Barbosa-Pereira; Giuseppe Zeppa. 2020. "In Vitro Bioaccessibility and Functional Properties of Phenolic Compounds from Enriched Beverages Based on Cocoa Bean Shell." Foods 9, no. 6: 715.