The method of preparation of highly stable reproducible C₆₀ fullerene aqueous colloid solution is described. The structural organization of C₆₀ fullerenes in aqueous solution was studied and analyzed in detail using various techniques such as chemical analysis, UV/VIS spectroscopy, atomic force and scanning tunneling microscopy, dynamic light scattering, and zeta potential methods.

Toxicity of the C₆₀ fullerene aqueous colloid solution in vitro and in vivo experiments was studied. C₆₀ fullerene in the concentration range of 3.6-144 µg/mL demonstrated low toxicity against human embryonic kidney (HEK293) cells with 383.4 μg/mL IC₅₀ value. The acute toxicity of C₆₀ fullerene in a dose progression (75, 150, 300, 600, 1200 and 1800 mg/kg) was investigated at 14^th day after single intraperitoneal (i.p.) administration into mice. No toxic effect of С₆₀ fullerene in the dose range of 75-150 mg/kg was observed; the calculated LD₅₀ value was 721 mg/kg. The toxic effect of C₆₀ fullerene was observed at a concentration of 300 mg/kg and higher, it was accompanied with mice behavior disturbance, hematotoxicity and pathomorphological changes in spleen, hepatic and kidney tissues. In mice treated with 600 mg/kg of C₆₀ fullerene indices of inflammatory process were detected, whereas at 1200 mg/kg dose leukopoies and thrombocytopoies were found to be suppressed. Thus, C₆₀ fullerene aqueous colloid solution in a dose range of 75-150 mg/kg is nontoxic and could be used for biomedical purposes.

Unusual thermodynamic properties of C₆₀ fullerene complexation with antibiotic doxorubicin were observed and explained.

Molybdenum disulfide (MoS₂) was recently found to exhibit an indirect-to-direct gap transition when its thickness is reduced to a single S–Mo–S trilayer sheet, which opens up opportunities for a range of novel optoelectronic applications. Here we first report the possibility of dispersant-free efficient liquid exfoliation down to the graphene-like state of solvent-free mechanochemically delaminated bulk MoS₂. The graphene-like, single-sheet structure of MoS₂ nanoparticles in the dispersions is confirmed by mutually complementary experimental methods. The proposed approach essentially improves the known liquid exfoliation method based on the ultrasound disintegration of bulk MoS₂, because the proposed mechanochemical treatment of the initial bulk MoS₂ could provide 100% yield of the single-sheet nanoparticles without the use of dispersants.

Among metal-based nanoparticles, silver nanoparticles (AgNPs) are particularly appealing because of their stability, functionality, and documented antimicrobial properties. AgNPs also offer the possibility of different surface modifications. In this work, we functionalized AgNPs with thiobarbituric acid or 11-mercaptoundecanoic acid residues to improve the nanoparticles’ biological activities. Subsequently, we assessed the physicochemical properties of newly synthesized AgNPs using a wide range of biophysical methodologies, including UV/vis and fluorescence spectroscopy, atomic force and scanning electron microscopy, and dynamic light scattering and isothermal titration calorimetry. Next, we examined the effect of nanoparticles functionalization on AgNPs mutagenicity and toxicity. Our study revealed that AgNPs’ surface modification affects nanoparticles aggregation, and also impacts nanoparticles’ interaction with model acridine mutagen ICR-191. AgNPs coated with MUA showed the most interesting interactions with tested ICR-191, slightly modulating its toxicity properties by decreasing the viability in treated cells.