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The viral family Coronaviridae comprises four genera, termed Alpha-, Beta-, Gamma-, and Deltacoronavirus. Recombination events have been described in many coronaviruses infecting humans and other animals. However, formal analysis of the recombination patterns, both in terms of the involved genome regions and the extent of genetic divergence between partners, are scarce. Common methods of recombination detection based on phylogenetic incongruences (e.g., a phylogenetic compatibility matrix) may fail in cases where too many events diminish the phylogenetic signal. Thus, an approach comparing genetic distances in distinct genome regions (pairwise distance deviation matrix) was set up. In alpha, beta, and delta-coronaviruses, a low incidence of recombination between closely related viruses was evident in all genome regions, but it was more extensive between the spike gene and other genome regions. In contrast, avian gammacoronaviruses recombined extensively and exist as a global cloud of genes with poorly corresponding genetic distances in different parts of the genome. Spike, but not other structural proteins, was most commonly exchanged between coronaviruses. Recombination patterns differed between coronavirus genera and corresponded to the modular structure of the spike: recombination traces were more pronounced between spike domains (N-terminal and C-terminal parts of S1 and S2) than within domains. The variability of possible recombination events and their uneven distribution over the genome suggest that compatibility of genes, rather than mechanistic or ecological limitations, shapes recombination patterns in coronaviruses.
Yulia Vakulenko; Andrei Deviatkin; Jan Drexler; Alexander Lukashev. Modular Evolution of Coronavirus Genomes. Viruses 2021, 13, 1270 .
AMA StyleYulia Vakulenko, Andrei Deviatkin, Jan Drexler, Alexander Lukashev. Modular Evolution of Coronavirus Genomes. Viruses. 2021; 13 (7):1270.
Chicago/Turabian StyleYulia Vakulenko; Andrei Deviatkin; Jan Drexler; Alexander Lukashev. 2021. "Modular Evolution of Coronavirus Genomes." Viruses 13, no. 7: 1270.
Aim. To evaluate the efficiency of bone repair on a critical-sized rat calvarial defect model using our original xenogeneic bone mineral, widely established Geistlich Bio-Oss®, and autologous bone graft. Materials and Methods. We created a critical-sized calvarial defect in Sprague-Dawley rats (n = 48) and then divided them into 4 groups (unfilled defect, autologous bone graft, Geistlich BioOss® and our original xenogeneic bone mineral, 12 rats per group). Rats were sacrificed upon 4 and 12 months (6 rats per time point) with the following excision of the implant and adjacent tissues. 3D structure, extent of mineralisation, and bone volume were measured by means of microcomputed tomography. Microanatomy of the explants and adjacent tissue was investigated by haematoxylin and eosin staining. Results. The highest and the lowest bone volume was expectedly detected when the defect was filled with the autologous bone graft or remained unfilled, respectively. Replacement of the defect by the original bone mineral entailed better regeneration as compared to Geistlich Bio-Oss. Bone mineral density, bone thickness and the extent of mineralisation did not differ significantly between the experimental groups and were close to the positive control values, indicating efficient bone repair. Conclusions. Original xenogeneic bone mineral promotes induction of bone regeneration as compared to Geistlich Bio-Oss®, a commercially available bone mineral widely used in the clinical practice.
A. V. Veremeev; R. N. Bolgarin; V. G. Nesterenko; A. A. Andreev-Andrievskiy. Xenogeneic bone mineral is efficient for the repair of critical-sized rat calvarial defects. Fundamental and Clinical Medicine 2021, 6, 16-26 .
AMA StyleA. V. Veremeev, R. N. Bolgarin, V. G. Nesterenko, A. A. Andreev-Andrievskiy. Xenogeneic bone mineral is efficient for the repair of critical-sized rat calvarial defects. Fundamental and Clinical Medicine. 2021; 6 (1):16-26.
Chicago/Turabian StyleA. V. Veremeev; R. N. Bolgarin; V. G. Nesterenko; A. A. Andreev-Andrievskiy. 2021. "Xenogeneic bone mineral is efficient for the repair of critical-sized rat calvarial defects." Fundamental and Clinical Medicine 6, no. 1: 16-26.
Molecular phylogenetics, particularly statistical phylogenetics, is widely used to solve the fundamental and applied problems in virology. Bayesian, or statistical, phylogenetic methods, which came into practice 10—15 years ago, markedly expanded the range of questions that can be answered based on analyzing nucleotide and amino acid sequences. An opportunity of using various evolution models allows inferring the chronology, geography and dynamics of the infection spreading. For example, analysis of globally distributed HIV group M by Bayesian methods demonstrated with a probability of 99% that the most recent common ancestor of these viruses existed in the surroundings of the city of Kinshasa (Democratic Republic of the Congo) in the early 1920s. Another study showed that H9N2 influenza virus most likely passed on to humans from wild ducks in Hong Kong in the late 1960s. In addition, using of the Bayesian analysis allows to evaluating the effect of measures taken on the development of the epidemic process. For example, it was shown retrospectively that the rate of hepatitis C virus infection cases in Egypt increased by several orders of magnitude in the mid-20th century. A sharp rise in new case rate is associated with the treatment for schistosomiasis by using non-sterile repeatedly used syringes. A set of Bayesian analysis methods has been applied in tens of thousands of researches describing various aspects of the occurrence and spread of infectious diseases in humans and animals. This was facilitated by the development and accessibility of software that implements these methods. The complexity of Bayesian phylogenetic methods imposes strict requirements on the data being analyzed. The correctness of the phylogenetic analysis data depends on various factors. For example, it is necessary to choose an evolutionary model that most adequately describes the studied objects. A mandatory step in formulating the results is the justification of the selected model. For viruses, the acquisition of genetic elements from other organisms is typical, therefore, the genomes even from closely related viruses may have non-homologous regions unsuitable for phylogenetic analysis. Another aspect is the creation of a representative dataset. Sometimes, all stages of the analysis are not indicated in publications, so that the data obtained can be interpreted ambiguously. The correct use of statistical phylogenetics methods in virology is possible only upon understanding their principles, proper methods of data preparation and evolutionary model selection criteria.
Yu. A. Vakulenko; A. N. Lukashev; A. A. Deviatkin. The use of statistical phylogenetics in virology. Russian Journal of Infection and Immunity 2021, 11, 42 -56.
AMA StyleYu. A. Vakulenko, A. N. Lukashev, A. A. Deviatkin. The use of statistical phylogenetics in virology. Russian Journal of Infection and Immunity. 2021; 11 (1):42-56.
Chicago/Turabian StyleYu. A. Vakulenko; A. N. Lukashev; A. A. Deviatkin. 2021. "The use of statistical phylogenetics in virology." Russian Journal of Infection and Immunity 11, no. 1: 42-56.
Imatinib mesilate (Glivec) is a well-known antitumor target inhibitor of protein tyrosine kinase, which is effective in different cancer types expressing Bcr / Abl and, in particular, in hemoblastosis. A higher interest to imatinib during the COVID-19 epidemic is explained by the fact that cancer patients are one of the COVID-19 risk groups. Moreover, imatinib target mechanism of action, which is effective in cancer, can have a high potential against the most severe COVID-19 complication such as the disease associated pulmonary fibrosis. COVID-19 associated interstitial pulmonary fibrosis develops as an autoimmune process caused by systemic inflammation with atypical (idiopathic) pneumonia resulting from acute respiratory distress syndrome with the tyrosine kinase mechanism of signaling pathway activation and cellular response. Experimental and clinical results showing antifibrotic and dose-related antithrombotic imatinib effect demonstrate perspective use of this antitumor agent to correct COVID-19 associated pneumonia causing a high death rate of patients with COVID-19.The review presents literature data of 2001–2020 discussing pathologic genetic and clinical characteristics of the fibrosis which exacerbates COVID-19 pneumonia in adults. The sequence of the disease processes demonstrates that disease progression with the decreasing oxygen saturation in the peripheral blood intensifies local thrombosis in the lungs. As a result, hypoxia is developing, which is difficult to control and can cause lethal outcome in severe cases. Yet, the conventional antifibrotic and thrombolytic agents can only partially control the process of pneumofibrosis including that of cancer patients. The approximate antifibrotic dose of imatinib 400 mg / day is therapeutic for oncopathology. The antitumor drug registered in many countries and well described side effects and contraindications needs no long-term registration studies for a new indication, therefore, it may be easily prepared for clinical testing.
I. N. Mikhaylova; N. M. Treshalina; I. Zh. Shubina; I. V. Manina; М. В. Киселевский; A. N. Lukashev. Antitumor proteinkinase inhibitor imatinib may be regarded as a potential correcting agent for COVID-19 associated pulmonary fibrosis. Advances in Molecular Oncology 2021, 7, 20-28 .
AMA StyleI. N. Mikhaylova, N. M. Treshalina, I. Zh. Shubina, I. V. Manina, М. В. Киселевский, A. N. Lukashev. Antitumor proteinkinase inhibitor imatinib may be regarded as a potential correcting agent for COVID-19 associated pulmonary fibrosis. Advances in Molecular Oncology. 2021; 7 (4):20-28.
Chicago/Turabian StyleI. N. Mikhaylova; N. M. Treshalina; I. Zh. Shubina; I. V. Manina; М. В. Киселевский; A. N. Lukashev. 2021. "Antitumor proteinkinase inhibitor imatinib may be regarded as a potential correcting agent for COVID-19 associated pulmonary fibrosis." Advances in Molecular Oncology 7, no. 4: 20-28.
The fungal glycoprotein l-lysine α-oxidase (LO) catalyzes the oxidative deamination of l-lysine (l-lys). LO may be internalized in the intestine and shows antitumor, antibacterial, and antiviral effects in vivo. The main mechanisms of its effects have been shown to be depletion of the essential amino acid l-lys and action of reactive oxidative species produced by the reaction. Here, we report that LO penetrates into the brain and is retained there for up to 48 h after intravenous injection, which might be explained by specific pharmacokinetics. LO actively intervenes in amino acid metabolism in the brain. The most significant impact of LO was towards amino acids, which are directly exposed to its action (l-lys, l-orn, l-arg). In addition, the enzyme significantly affected the redistribution of amino acids directly associated with the tricarboxylic acid (TCA) cycle (l-asp and l-glu). We discovered that the depletion of l-orn, the precursor of polyamines (PA), led to a significant and long-term decrease in the concentration of polyamines, which are responsible for regulation of many processes including cell proliferation. Thus, LO may be used to reduce levels of l-lys and PA in the brain.
Elena Lukasheva; Marina Makletsova; Alexander Lukashev; Gulalek Babayeva; Anna Arinbasarova; Alexander Medentsev. Fungal Enzyme l-Lysine α-Oxidase Affects the Amino Acid Metabolism in the Brain and Decreases the Polyamine Level. Pharmaceuticals 2020, 13, 398 .
AMA StyleElena Lukasheva, Marina Makletsova, Alexander Lukashev, Gulalek Babayeva, Anna Arinbasarova, Alexander Medentsev. Fungal Enzyme l-Lysine α-Oxidase Affects the Amino Acid Metabolism in the Brain and Decreases the Polyamine Level. Pharmaceuticals. 2020; 13 (11):398.
Chicago/Turabian StyleElena Lukasheva; Marina Makletsova; Alexander Lukashev; Gulalek Babayeva; Anna Arinbasarova; Alexander Medentsev. 2020. "Fungal Enzyme l-Lysine α-Oxidase Affects the Amino Acid Metabolism in the Brain and Decreases the Polyamine Level." Pharmaceuticals 13, no. 11: 398.
Currently, the lowest formal taxon in virus classification is species; however, unofficial lower-level units are commonly used in everyday work. Tick-borne encephalitis virus (TBEV) is a species of mammalian tick-borne flaviviruses that may cause encephalitis. Many known representatives of TBEV are grouped into subtypes, mostly according to their phylogenetic relationship. However, the emergence of novel sequences could dissolve this phylogenetic grouping; in the absence of strict quantitative criterion, it may be hard to define the borders of the first TBEV taxonomic unit below the species level. In this study, the nucleotide/amino-acid space of all known TBEV sequences was analyzed. Amino-acid sequence p-distances could not reliably distinguish TBEV subtypes. Viruses that differed by less than 10% of nucleotides in the polyprotein-coding gene belonged to the same subtype. At the same time, more divergent viruses were representatives of different subtypes. According to this distance criterion, TBEV species may be divided into seven subtypes: TBEV-Eur, TBEV-Sib, TBEV-FE, TBEV-2871 (TBEV-Ob), TBEV-Him, TBEV-178-79 (TBEV-Bkl-1), and TBEV-886-84 (TBEV-Bkl-2).
Andrei Deviatkin; Galina Karganova; Yulia Vakulenko; Alexander Lukashev. TBEV Subtyping in Terms of Genetic Distance. Viruses 2020, 12, 1240 .
AMA StyleAndrei Deviatkin, Galina Karganova, Yulia Vakulenko, Alexander Lukashev. TBEV Subtyping in Terms of Genetic Distance. Viruses. 2020; 12 (11):1240.
Chicago/Turabian StyleAndrei Deviatkin; Galina Karganova; Yulia Vakulenko; Alexander Lukashev. 2020. "TBEV Subtyping in Terms of Genetic Distance." Viruses 12, no. 11: 1240.
Rheumatoid arthritis (RA) is the most common autoimmune disease worldwide. Epigenetic alternations of microRNAs (miRNAs) can contribute to its pathogenesis and progression. As the first line therapy with DMARDs is not always successful, other drugs and therapeutic targets should be applied. This study aims to measure the expression level of plasma miRNAs in RA patients treated with olokizumab and to evaluate their potential as prognostic biomarkers. The expression of 9 miRNAs was quantified in 103 RA patients before treatment and at weeks 12 and 24 of olokizumab therapy by reverse transcription-polymerase chain reaction (RT-PCR) assay and analyzed in groups of responders and non-responders. Almost all miRNAs changed their expression during therapy. The ROC curve analysis of the most prominent of them together with consequent univariate and multivariate regression analysis revealed statistically significant associations with the olokizumab therapy efficiency scores for miR-26b, miR-29, miR-451, and miR-522. Therefore, these miRNAs might be a potential therapeutic response biomarker.
Irina Bure; Dmitry Mikhaylenko; Ekaterina Kuznetsova; Ekaterina Alekseeva; Kristina Bondareva; Alexey Kalinkin; Alexander Lukashev; Vadim Tarasov; Andrey Zamyatnin; Marina Nemtsova. Analysis of miRNA Expression in Patients with Rheumatoid Arthritis during Olokizumab Treatment. Journal of Personalized Medicine 2020, 10, 205 .
AMA StyleIrina Bure, Dmitry Mikhaylenko, Ekaterina Kuznetsova, Ekaterina Alekseeva, Kristina Bondareva, Alexey Kalinkin, Alexander Lukashev, Vadim Tarasov, Andrey Zamyatnin, Marina Nemtsova. Analysis of miRNA Expression in Patients with Rheumatoid Arthritis during Olokizumab Treatment. Journal of Personalized Medicine. 2020; 10 (4):205.
Chicago/Turabian StyleIrina Bure; Dmitry Mikhaylenko; Ekaterina Kuznetsova; Ekaterina Alekseeva; Kristina Bondareva; Alexey Kalinkin; Alexander Lukashev; Vadim Tarasov; Andrey Zamyatnin; Marina Nemtsova. 2020. "Analysis of miRNA Expression in Patients with Rheumatoid Arthritis during Olokizumab Treatment." Journal of Personalized Medicine 10, no. 4: 205.
Tick-Borne Encephalitis Virus (TBEV) is a dangerous arbovirus widely distributed in Northern Eurasia. The area of this pathogen changes over time. At the beginning of the 2000s, the Ixodes tick populations in Karelia increased. At the same time, the area of I. persulcatus, the main vector of the Siberian TBEV subtype, also expanded. Herein, we sequenced 10 viruses isolated from ticks collected in three locations from the Karelia region in 2008–2018. PCR positive samples were passaged in suckling mice or pig embryo kidney cells (PEK). After the second passage in suckling, mice viral RNA was isolated and E-gene fragment was sequenced. Viral sequences were expected to be similar or nearly identical. Instead, there was up to a 4.8% difference in nucleotide sequence, comparable with the most diverse viruses belonging to the Baltic subgroup in Siberian TBEV subtype (Baltic TBEV-Sib). To reveal whether this was systemic or incidental, a comprehensive phylogeographical analysis was conducted. Interestingly, viruses within each geographic region demonstrated comparable diversity to the whole Baltic TBEV-Sib. Moreover, Baltic TBEV-Sib has a distribution area limited by three ecological regions. This means that active virus mixing occurs in the vast geographic area forming one common virus pool. The most plausible explanation is the involvement of flying animals in the TBEV spread.
Andrei A. Deviatkin; Ivan S. Kholodilov; Oxana A. Belova; Sergey V. Bugmyrin; Lubov A. Bespyatova; Anna Y. Ivannikova; Yulia A. Vakulenko; Alexander N. Lukashev; Galina G. Karganova. Baltic Group Tick-Borne Encephalitis Virus Phylogeography: Systemic Inconsistency Pattern between Genetic and Geographic Distances. Microorganisms 2020, 8, 1589 .
AMA StyleAndrei A. Deviatkin, Ivan S. Kholodilov, Oxana A. Belova, Sergey V. Bugmyrin, Lubov A. Bespyatova, Anna Y. Ivannikova, Yulia A. Vakulenko, Alexander N. Lukashev, Galina G. Karganova. Baltic Group Tick-Borne Encephalitis Virus Phylogeography: Systemic Inconsistency Pattern between Genetic and Geographic Distances. Microorganisms. 2020; 8 (10):1589.
Chicago/Turabian StyleAndrei A. Deviatkin; Ivan S. Kholodilov; Oxana A. Belova; Sergey V. Bugmyrin; Lubov A. Bespyatova; Anna Y. Ivannikova; Yulia A. Vakulenko; Alexander N. Lukashev; Galina G. Karganova. 2020. "Baltic Group Tick-Borne Encephalitis Virus Phylogeography: Systemic Inconsistency Pattern between Genetic and Geographic Distances." Microorganisms 8, no. 10: 1589.
CRISPR/Cas technologies have advanced dramatically in recent years. Many different systems with new properties have been characterized and a plethora of hybrid CRISPR/Cas systems able to modify the epigenome, regulate transcription, and correct mutations in DNA and RNA have been devised. However, practical application of CRISPR/Cas systems is severely limited by the lack of effective delivery tools. In this review, recent advances in developing vehicles for the delivery of CRISPR/Cas in the form of ribonucleoprotein complexes are outlined. Most importantly, we emphasize the use of extracellular vesicles (EVs) for CRISPR/Cas delivery and describe their unique properties: biocompatibility, safety, capacity for rational design, and ability to cross biological barriers. Available molecular tools that enable loading of desired protein and/or RNA cargo into the vesicles in a controllable manner and shape the surface of EVs for targeted delivery into specific tissues (e.g., using targeting ligands, peptides, or nanobodies) are discussed. Opportunities for both endogenous (intracellular production of CRISPR/Cas) and exogenous (post-production) loading of EVs are presented.
Dmitry Kostyushev; Anastasiya Kostyusheva; Sergey Brezgin; Valery Smirnov; Elena Volchkova; Alexander Lukashev; Vladimir Chulanov. Gene Editing by Extracellular Vesicles. International Journal of Molecular Sciences 2020, 21, 7362 .
AMA StyleDmitry Kostyushev, Anastasiya Kostyusheva, Sergey Brezgin, Valery Smirnov, Elena Volchkova, Alexander Lukashev, Vladimir Chulanov. Gene Editing by Extracellular Vesicles. International Journal of Molecular Sciences. 2020; 21 (19):7362.
Chicago/Turabian StyleDmitry Kostyushev; Anastasiya Kostyusheva; Sergey Brezgin; Valery Smirnov; Elena Volchkova; Alexander Lukashev; Vladimir Chulanov. 2020. "Gene Editing by Extracellular Vesicles." International Journal of Molecular Sciences 21, no. 19: 7362.
Aim. To evaluate the efficacy of heterologous demineralised bone matrix (DBM) for the replacement of bone defects using a critical-sized rat calvarial defect model. Materials and Methods. For the experiments, we used 48 Sprague-Dawley rats (4.5 to 6 months of age). Critical-sized (8 mm diameter) calvarial defect was filled by the bone autograft, heterologous DBM, or comparator product (Geistlich BioOss®) or remained unfilled (negative control). Upon 4 or 12 weeks, rats were euthanised with the subsequent investigation of the defect and adjacent tissues by means of hematoxylin and eosin staining (mineralized tissue area to the defect area ratio) and microcomputed tomography (volume, thickness, and mineral density of the repaired tissue). Results. In our experimental setting, bone autograft was the most efficient in bone repair. Heterologous DBM and comparator product were equally efficient in filling the defect and did not show any statistically significant differences regarding any of the parameters. Microcomputed tomography and routine histological examination demonstrated concordant results. Conclusion. Heterologous DBM is efficient for the repair of critical-sized rat calvarial defects.
A. V. Veremeev; R. N. Bolgarin; V. G. Nesterenko; A. A. Andreev-Andrievskiy. Heterologous demineralised bone matrix is efficient for the repair of critical-sized rat calvarial defects. Fundamental and Clinical Medicine 2020, 5, 24-34 .
AMA StyleA. V. Veremeev, R. N. Bolgarin, V. G. Nesterenko, A. A. Andreev-Andrievskiy. Heterologous demineralised bone matrix is efficient for the repair of critical-sized rat calvarial defects. Fundamental and Clinical Medicine. 2020; 5 (3):24-34.
Chicago/Turabian StyleA. V. Veremeev; R. N. Bolgarin; V. G. Nesterenko; A. A. Andreev-Andrievskiy. 2020. "Heterologous demineralised bone matrix is efficient for the repair of critical-sized rat calvarial defects." Fundamental and Clinical Medicine 5, no. 3: 24-34.
Bats host many viruses pathogenic to humans, and increasing evidence suggests that Rotavirus A (RVA) also belongs to this list. Rotaviruses cause diarrheal disease in many mammals and birds, and their segmented genomes allow them to reassort and increase their genetic diversity. Eighteen out of 2,142 bat fecal samples (0.8%) collected from Europe, Central America and Africa were PCR-positive for RVA and 11 of those were fully characterized using viral metagenomics. Upon contrasting their genomes with publicly available data, at least 7 distinct bat RVA genotype constellations (GCs) were identified, including evidence of reassortments and 6 novel genotypes. Some of these constellations are spread across the world, whereas others appear to be geographically restricted. Our analyses also suggest that several unusual human and equine RVA strains might be of bat RVA origin, based on their phylogenetic clustering, despite varying levels of nucleotide sequence identities between them. Although SA11 is one of the most widely used reference strains for RVA research and forms the backbone of a reverse genetics system, its origin remained enigmatic. Remarkably, the majority of the genotypes of SA11-like strains were shared with Gabonese bat RVAs, suggesting a potential common origin. Overall, our findings suggest an underexplored genetic diversity of RVAs in bats, which is likely only the tip of the iceberg. Increasing contact between humans and bat wildlife will further increase the zoonosis risk, which warrants closer attention to these viruses. Importance The increased research on bat coronaviruses after SARS-CoV and MERS-CoVallowed the very rapid identification of SARS-CoV-2. This is an excellent example of the importance of knowing viruses harbored by wildlife in general and bats in particular, for global preparedness against emerging viral pathogens. The current effort to characterize bat rotavirus strains from 3 continents shed light on the vast genetic diversity of rotaviruses and also hinted at a bat origin for several atypical rotaviruses in humans and animals, implying that zoonoses of bat rotaviruses might occur more frequently than currently realized.
Ceren Simsek; Victor Max Corman; Hermann Ulrich Everling; Alexander N. Lukashev; Andrea Rasche; Gael Darren Maganga; Tabea Binger; Daan Jansen; Leen Beller; Ward Deboutte; Florian Gloza-Rausch; Antje Seebens-Hoyer; Stoian Yordanov; Augustina Sylverken; Samuel Kinsley Oppong; Yaw Adu Sarkodie; Peter Vallo; Eric M. Leroy; Mathieu Bourgarel; Kwe Claude Yinda; Marc Van Ranst; Christian Drosten; Jan Felix Drexler; Jelle Matthijnssens. At least seven distinct rotavirus genotype constellations in bats with evidence of reassortment and zoonotic transmissions. 2020, 1 .
AMA StyleCeren Simsek, Victor Max Corman, Hermann Ulrich Everling, Alexander N. Lukashev, Andrea Rasche, Gael Darren Maganga, Tabea Binger, Daan Jansen, Leen Beller, Ward Deboutte, Florian Gloza-Rausch, Antje Seebens-Hoyer, Stoian Yordanov, Augustina Sylverken, Samuel Kinsley Oppong, Yaw Adu Sarkodie, Peter Vallo, Eric M. Leroy, Mathieu Bourgarel, Kwe Claude Yinda, Marc Van Ranst, Christian Drosten, Jan Felix Drexler, Jelle Matthijnssens. At least seven distinct rotavirus genotype constellations in bats with evidence of reassortment and zoonotic transmissions. . 2020; ():1.
Chicago/Turabian StyleCeren Simsek; Victor Max Corman; Hermann Ulrich Everling; Alexander N. Lukashev; Andrea Rasche; Gael Darren Maganga; Tabea Binger; Daan Jansen; Leen Beller; Ward Deboutte; Florian Gloza-Rausch; Antje Seebens-Hoyer; Stoian Yordanov; Augustina Sylverken; Samuel Kinsley Oppong; Yaw Adu Sarkodie; Peter Vallo; Eric M. Leroy; Mathieu Bourgarel; Kwe Claude Yinda; Marc Van Ranst; Christian Drosten; Jan Felix Drexler; Jelle Matthijnssens. 2020. "At least seven distinct rotavirus genotype constellations in bats with evidence of reassortment and zoonotic transmissions." , no. : 1.
Here we evaluated the efficacy of bone repair using various native bovine biomaterials (refined hydroxyapatite (HA), demineralised bone matrix (DBM), and purified bone collagen (COLL)) as compared with commercially available bone mineral and bone autografts. We employed a conventional critical-sized (8 mm diameter) rat calvarial defect model (6-month-old male Sprague–Dawley rats, n = 72 in total). The artificial defect was repaired using HA, DBM, COLL, commercially available bone mineral powder, bone calvarial autograft, or remained unfilled (n = 12 animals per group). Rats were euthanised 4 or 12 weeks postimplantation (n = 6 per time point) with the subsequent examination to assess the extent, volume, area, and mineral density of the repaired tissue by means of microcomputed tomography and hematoxylin and eosin staining. Bovine HA and DBM powder exhibited excellent repair capability similar to the autografts and commercially available bone mineral powder while COLL showed higher bone repair rate. We suggest that HA and DBM powder obtained from bovine bone tissue can be equally applied for the repair of bone defects and demonstrate sufficient potential to be implemented into clinical studies.
Alexey Veremeev; Roman Bolgarin; Vladimir Nesterenko; Alexander Andreev-Andrievskiy; Anton Kutikhin. Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes are Efficient in Repair of Critical-Sized Rat Calvarial Defects. Materials 2020, 13, 3393 .
AMA StyleAlexey Veremeev, Roman Bolgarin, Vladimir Nesterenko, Alexander Andreev-Andrievskiy, Anton Kutikhin. Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes are Efficient in Repair of Critical-Sized Rat Calvarial Defects. Materials. 2020; 13 (15):3393.
Chicago/Turabian StyleAlexey Veremeev; Roman Bolgarin; Vladimir Nesterenko; Alexander Andreev-Andrievskiy; Anton Kutikhin. 2020. "Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes are Efficient in Repair of Critical-Sized Rat Calvarial Defects." Materials 13, no. 15: 3393.
Rheumatoid arthritis (RA) is the most common inflammatory arthropathy worldwide. Possible manifestations of RA can be represented by a wide variability of symptoms, clinical forms, and course options. This multifactorial disease is triggered by a genetic predisposition and environmental factors. Both clinical and genealogical studies have demonstrated disease case accumulation in families. Revealing the impact of candidate gene missense variants on the disease course elucidates understanding of RA molecular pathogenesis. A multivariate genomewide association study (GWAS) based analysis identified the genes and signalling pathways involved in the pathogenesis of the disease. However, these identified RA candidate gene variants only explain 30% of familial disease cases. The genetic causes for a significant proportion of familial RA have not been determined until now. Therefore, it is important to identify RA risk groups in different populations, as well as the possible prognostic value of some genetic variants for disease development, progression, and treatment. Our review has two purposes. First, to summarise the data on RA candidate genes and the increased disease risk associated with these alleles in various populations. Second, to describe how the genetic variants can be used in the selection of drugs for the treatment of RA.
Dmitry S. Mikhaylenko; Marina V. Nemtsova; Irina V. Bure; Ekaterina B. Kuznetsova; Ekaterina A. Alekseeva; Vadim V. Tarasov; Alexander N. Lukashev; Marina I. Beloukhova; Andrei A. Deviatkin; Jr. Andrey A. Zamyatnin. Genetic Polymorphisms Associated with Rheumatoid Arthritis Development and Antirheumatic Therapy Response. International Journal of Molecular Sciences 2020, 21, 4911 .
AMA StyleDmitry S. Mikhaylenko, Marina V. Nemtsova, Irina V. Bure, Ekaterina B. Kuznetsova, Ekaterina A. Alekseeva, Vadim V. Tarasov, Alexander N. Lukashev, Marina I. Beloukhova, Andrei A. Deviatkin, Jr. Andrey A. Zamyatnin. Genetic Polymorphisms Associated with Rheumatoid Arthritis Development and Antirheumatic Therapy Response. International Journal of Molecular Sciences. 2020; 21 (14):4911.
Chicago/Turabian StyleDmitry S. Mikhaylenko; Marina V. Nemtsova; Irina V. Bure; Ekaterina B. Kuznetsova; Ekaterina A. Alekseeva; Vadim V. Tarasov; Alexander N. Lukashev; Marina I. Beloukhova; Andrei A. Deviatkin; Jr. Andrey A. Zamyatnin. 2020. "Genetic Polymorphisms Associated with Rheumatoid Arthritis Development and Antirheumatic Therapy Response." International Journal of Molecular Sciences 21, no. 14: 4911.
Aim. To evaluate the efficacy of bone repair using xenogeneic native bone collagen (Bongraf COLLAGEN) as compared to other widely applied orthopaedic solutions (xenogeneic native bone mineral Geistlich Bio-Oss® and bone autograft).Materials and Methods. We employed a conventional critical-sized (8 mm) rat calvarial defect model (48 Wistar or Sprague-Dawley rats). The artificial defect was repaired using Bongraf COLLAGEN, Geistlich Bio-Oss® utilised as a comparator, bone calvarial autograft, or remained unfilled (n = 6 per group). Rats were euthanised 4 or 12 weeks postimplantation (n = 3 per time point) with the subsequent examination (repair extent, volume, thickness and mineral density of the repaired tissue) by means of microcomputed tomography and hematoxylin and eosin staining.Results. Expectedly, highest volume, thickness and mineral density of the repaired tissue have been observed in defects filled with autografts. Bongraf COLLAGEN and Geistlich Bio-Oss® also demonstrated a comparable and significant repair capability, yet the former option demonstrated higher bone regeneration rate.Conclusion. Xenogeneic native bone collagen (Bongraf COLLAGEN) is comparable with xenogeneic native bone mineral (Geistlich Bio-Oss®).
A. V. Veremeev; R. N. Bolgarin; V. G. Nesterenko; A. A. Andreev-Andrievskiy. Аpplication of xenogeneic native bone collagen for bone repair in critical-sized rat calvarial defect model. Fundamental and Clinical Medicine 2020, 5, 8-21 .
AMA StyleA. V. Veremeev, R. N. Bolgarin, V. G. Nesterenko, A. A. Andreev-Andrievskiy. Аpplication of xenogeneic native bone collagen for bone repair in critical-sized rat calvarial defect model. Fundamental and Clinical Medicine. 2020; 5 (2):8-21.
Chicago/Turabian StyleA. V. Veremeev; R. N. Bolgarin; V. G. Nesterenko; A. A. Andreev-Andrievskiy. 2020. "Аpplication of xenogeneic native bone collagen for bone repair in critical-sized rat calvarial defect model." Fundamental and Clinical Medicine 5, no. 2: 8-21.
Overall incidence of toxocariasis in Russia is low and varies between 1.6 and 2.7 per 100,000, while in several hyper-endemic regions, such as Altay, Kurgan, Perm and Udmurtia, it reaches 43 per 100,000. The seroprevalence of toxocariasis in published references was on average 16% and varied across the regions of Russia from negligible in North Siberia to 40% in southern regions of West Siberia. Seroprevalence in adults in five regions of Russia identified in this study was on average 20%, and varied from 3% in Yakutia (north of East Siberia) to 36% in Rostov-on-Don, South Russia. There was no correlation between seroprevalence and reported incidence of toxocariasis; however, the pattern of seroprevalence variation could be linked to Toxocara prevalence in dogs. Toxocariasis seroprevalence has more than doubled over the last 20 years. Diagnostic antibody titres (1:800 or more) were found in 3.6% of sera, suggesting about five million of acute Toxocara invasions per year.
Lyudmila V. Akhmadishina; Maria N. Ruzina; Maria A. Lukasheva; Karen K. Kyuregyan; Mikhail I. Mikhailov; Alexander N. Lukashev. Seroprevalence and incidence of human toxocarosis in Russia. Advances in Parasitology Volume 10 2020, 419 -432.
AMA StyleLyudmila V. Akhmadishina, Maria N. Ruzina, Maria A. Lukasheva, Karen K. Kyuregyan, Mikhail I. Mikhailov, Alexander N. Lukashev. Seroprevalence and incidence of human toxocarosis in Russia. Advances in Parasitology Volume 10. 2020; ():419-432.
Chicago/Turabian StyleLyudmila V. Akhmadishina; Maria N. Ruzina; Maria A. Lukasheva; Karen K. Kyuregyan; Mikhail I. Mikhailov; Alexander N. Lukashev. 2020. "Seroprevalence and incidence of human toxocarosis in Russia." Advances in Parasitology Volume 10 , no. : 419-432.
Despite the weak clinical efficacy of TRAIL death receptor agonists, a search is under way for new agents that more efficiently activate apoptotic signaling. We previously created a TRAIL DR5-selective variant DR5-B without affinity for the DR4, DcR1, DcR2, and OPG receptors and increased proapoptotic activity in tumor cells. Here we showed that DR5-B significantly inhibited tumor growth in HCT116 and Caco-2 but not in HT-29 xenografts. The antitumor activity of DR5-B was 2.5 times higher in HCT116 xenografts compared to TRAIL. DR5-B at a dose of 2 or 10 mg/kg/d for 10 days inhibited tumor growth in HCT116 xenografts by 26% or 50% respectively, and increased animal survival. Unexpectedly, DR5-B at a higher dose (25 mg/kg/d) inhibited tumor growth only during the first 8 days of drug exposure, while at the end of the monitoring, no effect or even slight stimulation of tumor growth was observed. The pharmacokinetic parameters of DR5-B were comparable to those of TRAIL, except that the half-life was 3.5 times higher. Thus, enhancing TRAIL selectivity to DR5 may increase both antitumor and proliferative activities depending on the concentration and administration regimens.
Anne V. Yagolovich; Artem A. Artykov; Tatiana A. Karmakova; Maria S. Vorontsova; Andrey A. Pankratov; Alexander Andreev-Andrievskiy; Dmitry A. Dolgikh; Mikhail Kirpichnikov; Marine E. Gasparian. Genetically Modified DR5-Specific TRAIL Variant DR5-B Revealed Dual Antitumor and Protumoral Effect in Colon Cancer Xenografts and an Improved Pharmacokinetic Profile. Translational Oncology 2020, 13, 100762 .
AMA StyleAnne V. Yagolovich, Artem A. Artykov, Tatiana A. Karmakova, Maria S. Vorontsova, Andrey A. Pankratov, Alexander Andreev-Andrievskiy, Dmitry A. Dolgikh, Mikhail Kirpichnikov, Marine E. Gasparian. Genetically Modified DR5-Specific TRAIL Variant DR5-B Revealed Dual Antitumor and Protumoral Effect in Colon Cancer Xenografts and an Improved Pharmacokinetic Profile. Translational Oncology. 2020; 13 (4):100762.
Chicago/Turabian StyleAnne V. Yagolovich; Artem A. Artykov; Tatiana A. Karmakova; Maria S. Vorontsova; Andrey A. Pankratov; Alexander Andreev-Andrievskiy; Dmitry A. Dolgikh; Mikhail Kirpichnikov; Marine E. Gasparian. 2020. "Genetically Modified DR5-Specific TRAIL Variant DR5-B Revealed Dual Antitumor and Protumoral Effect in Colon Cancer Xenografts and an Improved Pharmacokinetic Profile." Translational Oncology 13, no. 4: 100762.
Lynx1 is a GPI‐tethered protein colocalized with nicotinic acetylcholine receptors (nAChRs) in the brain areas important for learning and memory. Previously we demonstrated that at low micromolar concentrations the water‐soluble Lynx1 variant lacking GPI‐anchor (ws‐Lynx1) acts on α7‐nAChRs as a positive allosteric modulator. We hypothesized that ws‐Lynx1 could be used for improvement of cognitive processes dependent on nAChRs. Here we showed that 2 µM ws‐Lynx1 increased the acetylcholine‐evoked current at α7‐nAChRs in the rat primary visual cortex L1 interneurons. At higher concentrations ws‐Lynx1 inhibits α7‐nAChRs expressed in X. laevis oocytes with IC50 ~50 µM. In C57BL/6 mice, ws‐Lynx1 penetrated the blood‐brain barrier upon intranasal administration and accumulated in the cortex, hippocampus, and cerebellum. Chronic ws‐Lynx1 treatment prevented the olfactory memory and motor learning impairment induced by the α7‐nAChRs inhibitor methyllycaconitine (MLA). Enhanced long‐term potentiation (LTP) and increased paired‐pulse facilitation ratio were observed in the hippocampal slices incubated with ws‐Lynx1 and in the slices from ws‐Lynx1‐treated mice. LTP blockade observed in MLA‐treated mice was abolished by ws‐Lynx1 co‐administration. To understand the mechanism of ws‐Lynx1 action, we studied the interaction of ws‐Lynx1 and MLA at α7‐nAChRs, measured the basal concentrations of endogenous Lynx1 and the α7 nAChR subunit and their association in the mouse brain . Our findings suggest that endogenous Lynx1 limits α7‐nAChRs activation in the adult brain. Ws‐Lynx1 partially displaces Lynx1 causing positive modulation of α7‐nAChRs and enhancement of synaptic plasticity. Ws‐Lynx1 and similar compounds may constitute useful hits for treatment of cognitive deficits associated with the cholinergic system dysfunction.
Zakhar O. Shenkarev; Mikhail A. Shulepko; Maxim L. Bychkov; Dmitrii S. Kulbatskii; Olga V. Shlepova; Nathalia A. Vasilyeva; Alexander Andreev-Andrievskiy; Anfisa S. Popova; Evgeniya A. Lagereva; Eugene V. Loktyushov; Sergey G. Koshelev; Morten S. Thomsen; Dmitry A. Dolgikh; Sergey A. Kozlov; Pavel M. Balaban; Mikhail Kirpichnikov; Ekaterina N. Lyukmanova. Water‐soluble variant of human Lynx1 positively modulates synaptic plasticity and ameliorates cognitive impairment associated with α7‐nAChR dysfunction. Journal of Neurochemistry 2020, 155, 45 -61.
AMA StyleZakhar O. Shenkarev, Mikhail A. Shulepko, Maxim L. Bychkov, Dmitrii S. Kulbatskii, Olga V. Shlepova, Nathalia A. Vasilyeva, Alexander Andreev-Andrievskiy, Anfisa S. Popova, Evgeniya A. Lagereva, Eugene V. Loktyushov, Sergey G. Koshelev, Morten S. Thomsen, Dmitry A. Dolgikh, Sergey A. Kozlov, Pavel M. Balaban, Mikhail Kirpichnikov, Ekaterina N. Lyukmanova. Water‐soluble variant of human Lynx1 positively modulates synaptic plasticity and ameliorates cognitive impairment associated with α7‐nAChR dysfunction. Journal of Neurochemistry. 2020; 155 (1):45-61.
Chicago/Turabian StyleZakhar O. Shenkarev; Mikhail A. Shulepko; Maxim L. Bychkov; Dmitrii S. Kulbatskii; Olga V. Shlepova; Nathalia A. Vasilyeva; Alexander Andreev-Andrievskiy; Anfisa S. Popova; Evgeniya A. Lagereva; Eugene V. Loktyushov; Sergey G. Koshelev; Morten S. Thomsen; Dmitry A. Dolgikh; Sergey A. Kozlov; Pavel M. Balaban; Mikhail Kirpichnikov; Ekaterina N. Lyukmanova. 2020. "Water‐soluble variant of human Lynx1 positively modulates synaptic plasticity and ameliorates cognitive impairment associated with α7‐nAChR dysfunction." Journal of Neurochemistry 155, no. 1: 45-61.
Tick-borne encephalitis (TBE) is one of the most important viral zoonosis transmitted by the bite of infected ticks. In this study, all tick-borne encephalitis virus (TBEV) E gene sequences available in GenBank as of June 2019 with known date of isolation (n = 551) were analyzed. Simulation studies showed that a sample bias could significantly affect earlier studies, because small TBEV datasets (n = 50) produced non-overlapping intervals for evolutionary rate estimates. An apparent lack of a temporal signal in TBEV, in general, was found, precluding molecular clock analysis of all TBEV subtypes in one dataset. Within all subtypes and most of the smaller groups in these subtypes, there was evidence of many medium- and long-distance virus transfers. These multiple random events may play a key role in the virus spreading. For some groups, virus diversity within one territory was similar to diversity over the whole geographic range. This is best exemplified by the virus diversity observed in Switzerland or Czech Republic. These two countries yielded most of the known European subtype Eu3 subgroup sequences, and the diversity of viruses found within each of these small countries is comparable to that of the whole Eu3 subgroup, which is prevalent all over Central and Eastern Europe. Most of the deep tree nodes within all three established TBEV subtypes dated less than 300 years back. This could be explained by the recent emergence of most of the known TBEV diversity. Results of bioinformatics analysis presented here, together with multiple field findings, suggest that TBEV may be regarded as an emerging disease.
Andrei A. Deviatkin; Ivan S. Kholodilov; Yulia A. Vakulenko; Galina G. Karganova; Alexander N. Lukashev. Tick-Borne Encephalitis Virus: An Emerging Ancient Zoonosis? Viruses 2020, 12, 247 .
AMA StyleAndrei A. Deviatkin, Ivan S. Kholodilov, Yulia A. Vakulenko, Galina G. Karganova, Alexander N. Lukashev. Tick-Borne Encephalitis Virus: An Emerging Ancient Zoonosis? Viruses. 2020; 12 (2):247.
Chicago/Turabian StyleAndrei A. Deviatkin; Ivan S. Kholodilov; Yulia A. Vakulenko; Galina G. Karganova; Alexander N. Lukashev. 2020. "Tick-Borne Encephalitis Virus: An Emerging Ancient Zoonosis?" Viruses 12, no. 2: 247.
In this work, we describe the first Leishmania-infecting leishbunyavirus—the first virus other than Leishmania RNA virus (LRV) found in trypanosomatid parasites. Its host is Leishmania martiniquensis, a human pathogen causing infections with a wide range of manifestations from asymptomatic to severe visceral disease. This virus (LmarLBV1) possesses many characteristic features of leishbunyaviruses, such as tripartite organization of its RNA genome, with ORFs encoding RNA-dependent RNA polymerase, surface glycoprotein, and nucleoprotein on L, M, and S segments, respectively. Our phylogenetic analyses suggest that LmarLBV1 originated from leishbunyaviruses of monoxenous trypanosomatids and, probably, is a result of genomic re-assortment. The LmarLBV1 facilitates parasites’ infectivity in vitro in primary murine macrophages model. The discovery of a virus in L. martiniquensis poses the question of whether it influences pathogenicity of this parasite in vivo, similarly to the LRV in other Leishmania species.
Danyil Grybchuk; Diego H. Macedo; Yulia Kleschenko; Natalya Kraeva; Alexander N. Lukashev; Paul A. Bates; Pavel Kulich; Tereza Leštinová; Petr Volf; Alexei Y. Kostygov; Vyacheslav Yurchenko. The First Non-LRV RNA Virus in Leishmania. Viruses 2020, 12, 168 .
AMA StyleDanyil Grybchuk, Diego H. Macedo, Yulia Kleschenko, Natalya Kraeva, Alexander N. Lukashev, Paul A. Bates, Pavel Kulich, Tereza Leštinová, Petr Volf, Alexei Y. Kostygov, Vyacheslav Yurchenko. The First Non-LRV RNA Virus in Leishmania. Viruses. 2020; 12 (2):168.
Chicago/Turabian StyleDanyil Grybchuk; Diego H. Macedo; Yulia Kleschenko; Natalya Kraeva; Alexander N. Lukashev; Paul A. Bates; Pavel Kulich; Tereza Leštinová; Petr Volf; Alexei Y. Kostygov; Vyacheslav Yurchenko. 2020. "The First Non-LRV RNA Virus in Leishmania." Viruses 12, no. 2: 168.
Rheumatoid arthritis (RA) is a systemic inflammatory joint disease affecting about 1% of the population worldwide. Current treatment approaches do not ensure a cure for every patient. Moreover, classical regimens are based on nontargeted systemic immune suppression and have significant side effects. Biological treatment has advanced considerably but efficacy and specificity issues remain. Gene therapy is one of the potential future directions for RA therapy, which is rapidly developing. Several gene therapy trials done so far have been of moderate success, but experimental and genetics studies have yielded novel targets. As a result, the arsenal of gene therapy tools keeps growing. Currently, both viral and nonviral delivery systems are used for RA therapy. Herein, we review recent approaches for RA gene therapy.
Andrei A. Deviatkin; Yulia A. Vakulenko; Ludmila V. Akhmadishina; Vadim V. Tarasov; Marina Beloukhova; Andrey A. Zamyatnin Jr.; Alexander N. Lukashev. Emerging Concepts and Challenges in Rheumatoid Arthritis Gene Therapy. Biomedicines 2020, 8, 9 .
AMA StyleAndrei A. Deviatkin, Yulia A. Vakulenko, Ludmila V. Akhmadishina, Vadim V. Tarasov, Marina Beloukhova, Andrey A. Zamyatnin Jr., Alexander N. Lukashev. Emerging Concepts and Challenges in Rheumatoid Arthritis Gene Therapy. Biomedicines. 2020; 8 (1):9.
Chicago/Turabian StyleAndrei A. Deviatkin; Yulia A. Vakulenko; Ludmila V. Akhmadishina; Vadim V. Tarasov; Marina Beloukhova; Andrey A. Zamyatnin Jr.; Alexander N. Lukashev. 2020. "Emerging Concepts and Challenges in Rheumatoid Arthritis Gene Therapy." Biomedicines 8, no. 1: 9.