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Riccardo Pratesi
Interdisciplinary Laboratory of Biosciences and Celiac Disease Research Center, School of Medicine, University of Brasilia, Brasilia 70910-900, DF, Brazil

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https://pubmed.ncbi.nlm.nih.gov/30149623/

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Journal article
Published: 13 July 2021 in Brain Sciences
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Studies have shown that children and adolescents with autism and their relatives present a high level of stress and more family problems, impacting parents’ and caregivers’ quality of life (QoL). Despite studies on this subject, there is no specific questionnaire to evaluate QoL in parents or caregivers of children and adolescents with an autistic spectrum disorder (ASD) in Brazil. Therefore, this study’s primary purpose was to develop and validate a specific questionnaire to evaluate QoL in these individuals. The study was performed using the following steps: development of the ASD Parent/caregiver QoL questionnaire (autistic spectrum disorder parent/caregiver quality of life—ASDPC-QoL), subjective evaluation, validation of the questionnaire by the Delphi method, assessment of internal consistency, responsiveness, and reliability of the ASLPC-QoL, and administration of the questionnaire to 881 Brazilian ASD caregivers or parents. ASDPC-QoL comprises 28 questions divided into four domains (social, concerns, physical and mental health) with good psychometric properties (reproducibility, reliability, internal consistency, responsiveness, and validity). Our data showed that worries and physical health were the domains with the lowest scores in ASDPCA-QoL. ASDPCA-QoL did not differ among gender and age of child considering the total and all domains. Older participants (≥41 y/o) presented the best scores for social and worries domains but did not differ in other domains and the total. Parents or caregivers of ASD children diagnosed for more than three years have better mental and physical health domains than those recently diagnosed (up to 1 year) but did not differ in the total and other domains. Individuals with a partner and with the highest educational level present the best score for the social domain. Employed individuals showed better scores than unemployed ones for all domains and the total, except for worries, which did not differ. It also occurred comparing the individuals that do not use antidepressants and the ones that use them. Assessing and better understanding the QoL of caregivers is highly relevant. By understanding the social, worries, physical, and emotional health domains of caregivers, it is possible to track harmful aspects, prevent and treat pathologies, in addition to assisting in the implementation of effective public policies.

ACS Style

Claudia Pratesi; Alessandra Garcia; Riccardo Pratesi; Lenora Gandolfi; Mariana Hecht; Eduardo Nakano; Renata Zandonadi. Quality of Life in Caregivers of Children and Adolescents with Autistic Spectrum Disorder: Development and Validation of the Questionnaire. Brain Sciences 2021, 11, 924 .

AMA Style

Claudia Pratesi, Alessandra Garcia, Riccardo Pratesi, Lenora Gandolfi, Mariana Hecht, Eduardo Nakano, Renata Zandonadi. Quality of Life in Caregivers of Children and Adolescents with Autistic Spectrum Disorder: Development and Validation of the Questionnaire. Brain Sciences. 2021; 11 (7):924.

Chicago/Turabian Style

Claudia Pratesi; Alessandra Garcia; Riccardo Pratesi; Lenora Gandolfi; Mariana Hecht; Eduardo Nakano; Renata Zandonadi. 2021. "Quality of Life in Caregivers of Children and Adolescents with Autistic Spectrum Disorder: Development and Validation of the Questionnaire." Brain Sciences 11, no. 7: 924.

Communication
Published: 04 June 2021 in Sustainability
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Plastic pollution is a rapidly growing environmental and human health crisis, with no sign of improvement. From 2012 to 2020, the number of studies on plastic pollution increased, and macro to nano-sized plastics have been documented in the most remote biomes of the planet. Studies have shown contamination by microplastics (MPs) in various types of food consumed by humans, including seafood, honey, sugar, salt, tap and bottled water and beer. This study’s objective was to detect the possible contamination by MPs in drinking water samples collected from two main residential and commercial areas of Brasilia. A total of 32 samples (500 mL) of tap water were collected from residential and commercial areas. Samples were processed and transferred to a Sedgewick-Rafter counting cell chamber. The presence of MP particles was analyzed using a Nikon Eclipse fluorescence microscope. MPs were found in 100% of the samples. The mean microplastic particles per 500 mL found in the South Wing area was 97 ± 55, while the mean number of particles in the North Wing area was 219 ± 158, and the MPs found ranged in size from 6–50 microns. The study results reveal a disturbing amount of MP particles in Brasilia’s tap water. This surprising number of particles in residential and commercial tap water is especially considering that tap water is not the only source of MPs to which people are exposed.

ACS Style

Claudia Pratesi; Maria A. L. Santos Almeida; Geysa Cutrim Paz; Marcelo Ramos Teotonio; Lenora Gandolfi; Riccardo Pratesi; Mariana Hecht; Renata Zandonadi. Presence and Quantification of Microplastic in Urban Tap Water: A Pre-Screening in Brasilia, Brazil. Sustainability 2021, 13, 6404 .

AMA Style

Claudia Pratesi, Maria A. L. Santos Almeida, Geysa Cutrim Paz, Marcelo Ramos Teotonio, Lenora Gandolfi, Riccardo Pratesi, Mariana Hecht, Renata Zandonadi. Presence and Quantification of Microplastic in Urban Tap Water: A Pre-Screening in Brasilia, Brazil. Sustainability. 2021; 13 (11):6404.

Chicago/Turabian Style

Claudia Pratesi; Maria A. L. Santos Almeida; Geysa Cutrim Paz; Marcelo Ramos Teotonio; Lenora Gandolfi; Riccardo Pratesi; Mariana Hecht; Renata Zandonadi. 2021. "Presence and Quantification of Microplastic in Urban Tap Water: A Pre-Screening in Brasilia, Brazil." Sustainability 13, no. 11: 6404.

Journal article
Published: 09 May 2021 in Nutrients
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Since the end of 2019, the world has been facing an unpredicted COVID-19 pandemic with consequences for the economy, environment, society, and health. The COVID-19 pandemic has increased the risk of death, bringing unbearable psychological pressure upon people worldwide. For celiac patients, the pandemic may represent an additional burden concerning the inherent aspects of celiac disease (CD) that compromise these individuals’ quality of life (QoL). Therefore, the objective of this study was to evaluate Brazilian celiac patients’ QoL during the course of the COVID-19 pandemic caused by its outbreak and rapid spread and subsequent restrictive measures in addition to the dietary restrictions and other burdens caused by CD. This country-wide cross-sectional study was conducted using a self-administered instrument previously validated in Brazilian–Portuguese to investigate the QoL of individuals with CD. Data collected through the online self-administration of the Brazilian version of the celiac disease quality of life questionnaire (CDQ) comprised 674 CD individuals’ responses. Although pandemics have historically posed a challenge for Brazilian population, this period was not associated with a negative impact on Brazilian CD individuals’ QoL. During the pandemic, the QoL of Brazilian’s with CD was more affected by gastrointestinal aspects than emotions and social aspects and worries. Gender, age, marital status, having (or not) children, occupation, and a positive test for COVID-19 did not affect CD individuals’ QoL. However, the study revealed a larger burden and diminished QoL for individuals not following a gluten-free diet and those using antidepressants. Additional research is necessary to verify how the length of the pandemic will affect celiac individuals and then compare those outcomes compare to the COVID-19 period and after.

ACS Style

Ana Falcomer; Priscila Farage; Cláudia Pratesi; Riccardo Pratesi; Lenora Gandolfi; Eduardo Nakano; António Raposo; Renata Zandonadi. Health-Related Quality of Life and Experiences of Brazilian Celiac Individuals over the Course of the Sars-Cov-2 Pandemic. Nutrients 2021, 13, 1582 .

AMA Style

Ana Falcomer, Priscila Farage, Cláudia Pratesi, Riccardo Pratesi, Lenora Gandolfi, Eduardo Nakano, António Raposo, Renata Zandonadi. Health-Related Quality of Life and Experiences of Brazilian Celiac Individuals over the Course of the Sars-Cov-2 Pandemic. Nutrients. 2021; 13 (5):1582.

Chicago/Turabian Style

Ana Falcomer; Priscila Farage; Cláudia Pratesi; Riccardo Pratesi; Lenora Gandolfi; Eduardo Nakano; António Raposo; Renata Zandonadi. 2021. "Health-Related Quality of Life and Experiences of Brazilian Celiac Individuals over the Course of the Sars-Cov-2 Pandemic." Nutrients 13, no. 5: 1582.

Journal article
Published: 18 November 2020 in International Journal of Environmental Research and Public Health
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The COVID-19 outbreak, caused by Sars-Cov-2, was officially declared a global pandemic in February 2020, after an unexpected increase in hospitalization and mortality. When faced with this new disease, social and physical distancing and quarantine emerged as solutions to reduce virus transmission. This article examines the quality of life (QoL) of the Brazilian population’s during this period of isolation, due to the COVID-19 pandemic by analyzing; physical, psychological, social, and economic aspects. An online survey was distributed from 27 May to 14 August of 2020. A total of 1859 surveys were completed. Our results indicate that Brazilians were more affected by economic and social aspects than psychological and physical. Unemployed participants and individuals who tested positive for COVID-19 presented the lowest QoL. Females showed worst QoL scores than males, but having children did not influence the score. Higher educational level was associated with a better perception of QoL. Not following social distancing guidelines presented better scores in the psychological domain than the ones following restrict or partial social distancing rules. This study is the first to evaluate adults’ QoL related to the Sars-Cov-2 pandemic in Brazil at a national level. Our data may help health authorities identify the main factors affecting the QoL of the Brazilian population, thereby orientating them to recover after the pandemic.

ACS Style

Isabella Teotônio; Mariana Hecht; Luiz Claudio Castro; Lenora Gandolfi; Riccardo Pratesi; Eduardo Y. Nakano; Renata Puppin Zandonadi; Claudia B. Pratesi. Repercussion of COVID-19 Pandemic on Brazilians’ Quality of Life: A Nationwide Cross-Sectional Study. International Journal of Environmental Research and Public Health 2020, 17, 8554 .

AMA Style

Isabella Teotônio, Mariana Hecht, Luiz Claudio Castro, Lenora Gandolfi, Riccardo Pratesi, Eduardo Y. Nakano, Renata Puppin Zandonadi, Claudia B. Pratesi. Repercussion of COVID-19 Pandemic on Brazilians’ Quality of Life: A Nationwide Cross-Sectional Study. International Journal of Environmental Research and Public Health. 2020; 17 (22):8554.

Chicago/Turabian Style

Isabella Teotônio; Mariana Hecht; Luiz Claudio Castro; Lenora Gandolfi; Riccardo Pratesi; Eduardo Y. Nakano; Renata Puppin Zandonadi; Claudia B. Pratesi. 2020. "Repercussion of COVID-19 Pandemic on Brazilians’ Quality of Life: A Nationwide Cross-Sectional Study." International Journal of Environmental Research and Public Health 17, no. 22: 8554.

Journal article
Published: 26 September 2020 in International Journal of Environmental Research and Public Health
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This cross-sectional study aimed to translate, culturally adapt, validate, and apply a Celiac Disease Quality of Life (CD-QoL) questionnaire to a representative sample of Argentina’s celiac population. A previously developed and validated questionnaire (Celiac Disease Questionnaire: CDQ) was chosen as a tool for assessing the health-related quality of Life (HRQoL) of adult celiac patients in Argentina. Therefore, the study was performed in four stages: (a) translation and re-translation of the CDQ to Argentinian-Spanish language; (b) cultural adaptation and semantic evaluation; based on the Delphi method (c) validation of the CDQ by applying it to a representative sample of Argentinian celiac patients; (d) statistical analysis of the data. The result of stages (a) and (b) was a translated and culturally adapted an Argentinian-Spanish version of the CDQ, which was generated after reaching consensus between the corresponding four (phase a) and 10 (phase b) professionals involved in the different phases of this process. Among them, we can cite bilingual healthcare professionals with extensive experience in research and celiac disease, celiac patients, gastroenterologists, general practitioners, dieticians, and psychologists. The resulting CDQ proved to be an appropriate measuring tool to assess the HRQoL of Argentinian celiac patients confirmed by a good fit in the confirmatory factor validity analysis (RMSEA < 0.001 and χ2 = 267.325, df = 313, p = 0.971) and high values of internal consistency (Cronbach’s alpha > 0.7). A total of 191 participants accessed the questionnaire, and 171 individuals from 20 out of 23 Argentinian states completed the questionnaire. There was no correlation between higher educational level nor marital status with QoL. Individuals on a strict gluten-free diet (GFD) and those who do not take antidepressants showed higher QoL. Male gender also presented better HRQoL. There was no correlation between differences in HRQoL and age of the respondent, age at diagnosis, symptoms at diagnosis, or having other chronic diseases. However, a significantly higher score of HRQoL was reported among those individuals who disclosed having knowledge of CD related national regulations and benefits. This study highlights the importance of maintaining current public health regulations that support chronic disease patients, such as celiac patients.

ACS Style

Nicole Selleski; Renata Puppin Zandonadi; Laura B. Milde; Lenora Gandolfi; Riccardo Pratesi; Winfred Häuser; Rosa Harumi Uenishi; Eduardo Yoshio Nakano; Claudia B. Pratesi. Evaluation of Quality of Life of Adult Patients with Celiac Disease in Argentina: From Questionnaire Validation to Assessment. International Journal of Environmental Research and Public Health 2020, 17, 7051 .

AMA Style

Nicole Selleski, Renata Puppin Zandonadi, Laura B. Milde, Lenora Gandolfi, Riccardo Pratesi, Winfred Häuser, Rosa Harumi Uenishi, Eduardo Yoshio Nakano, Claudia B. Pratesi. Evaluation of Quality of Life of Adult Patients with Celiac Disease in Argentina: From Questionnaire Validation to Assessment. International Journal of Environmental Research and Public Health. 2020; 17 (19):7051.

Chicago/Turabian Style

Nicole Selleski; Renata Puppin Zandonadi; Laura B. Milde; Lenora Gandolfi; Riccardo Pratesi; Winfred Häuser; Rosa Harumi Uenishi; Eduardo Yoshio Nakano; Claudia B. Pratesi. 2020. "Evaluation of Quality of Life of Adult Patients with Celiac Disease in Argentina: From Questionnaire Validation to Assessment." International Journal of Environmental Research and Public Health 17, no. 19: 7051.

Journal article
Published: 25 March 2020 in Medicina
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Background and objectives: The present study is the first known in Latin America to enroll a substantial number of Kawasaki disease (KD) patients with an extended follow-up. This study aimed to: (1) to expose the difficulties and delays in the diagnosis of KD in a developing country, (2) to describe and correlate the clinical features of this disorder with the children’s age at the time of disease onset, (3) to correlate the frequent lack of early diagnosis with a delayed application of appropriate treatment, and (4) to describe the outcome and eventual recurrences of KD in our region. Materials and Methods: Three hundred and one participants (183 males and 118 females) included in the study were diagnosed and, subsequently, clinically followed for ten years (January 2007 to December 2016) at the Pediatric Rheumatology Walk-in Clinic of the Children’s Hospital of Brasilia. Results: Episodes ranged from four months to two years. This rate of recurrence was well-above that disclosed by previous reports. Delay in diagnosis, in all age groups, caused an undesirable delay between the disease onset, the final diagnosis, and the administration of intravenous immunoglobulin (IVIG). KD recurred in 25 (8.3%) of the children during the first three years of follow-up. In seven patients, KD recurred twice, with an interval between episodes ranging from four months to two years. Conclusions: This rate of recurrence was well-above that disclosed by previous reports. In Latin America, aside from a handful of physicians and researchers, KD is being ignored. There is a pressing need to educate primary health care physicians and bring awareness to the fact that KD is not an exotic condition that affects only the Asian populations but a disorder that already exists among us and that frequently results in severe consequences.

ACS Style

Cristina Medeiros R. De Magalhães; Fernanda Coutinho De Almeida; Lenora Gandolfi; Riccardo Pratesi; Natália Ribeiro De M. Alves; Nicole Selleski; Renata Puppin Zandonadi; Eduardo Yoshio Nakano; Claudia B. Pratesi. Clinical Manifestations of Kawasaki Disease at Different Age Spectrum: A Ten-Year Study. Medicina 2020, 56, 145 .

AMA Style

Cristina Medeiros R. De Magalhães, Fernanda Coutinho De Almeida, Lenora Gandolfi, Riccardo Pratesi, Natália Ribeiro De M. Alves, Nicole Selleski, Renata Puppin Zandonadi, Eduardo Yoshio Nakano, Claudia B. Pratesi. Clinical Manifestations of Kawasaki Disease at Different Age Spectrum: A Ten-Year Study. Medicina. 2020; 56 (4):145.

Chicago/Turabian Style

Cristina Medeiros R. De Magalhães; Fernanda Coutinho De Almeida; Lenora Gandolfi; Riccardo Pratesi; Natália Ribeiro De M. Alves; Nicole Selleski; Renata Puppin Zandonadi; Eduardo Yoshio Nakano; Claudia B. Pratesi. 2020. "Clinical Manifestations of Kawasaki Disease at Different Age Spectrum: A Ten-Year Study." Medicina 56, no. 4: 145.

Review article
Published: 10 December 2019 in International Journal for Parasitology: Drugs and Drug Resistance
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Chagas disease (CD) is a tropical neglected illness, affecting mainly populations of low socioeconomic status in Latin America. An estimated 6 to 8 million people worldwide are infected with Trypanosoma cruzi, the etiological agent of CD. Despite being one of the main global health problems, this disease continues without effective treatment during the chronic phase of the infection. The limitation of therapeutic strategies has been one of the biggest challenges on the fight against CD. Nifurtimox and benznidazole, developed in the 1970s, are still the only commercial options with established efficacy on CD. However, the efficacy of these drugs have a proven efficacy only during early infection and the benefits in the chronic phase are questionable. Consequently, there is a growing need for new pharmacological alternatives, either by optimization of existing drugs or by the formulation of new compounds. In the present study, a literature review of the currently adopted therapy, its concomitant combination with other drugs, and potential future treatments for CD was performed, considering articles published from 2012. The revised articles were selected according to the protocol of treatment: evaluation of drug association, drug repositioning and research of new drugs. As a result of the present revision, it was possible to conclude that the use of benznidazole in combination with other compounds showed better results when compared with its use as a single therapy. The search of new drugs has been the strategy most used in pursuing more effective forms of treatment for CD. However, studies have still focused on basic research, that is, they are still in a pre-clinical stage, using methodologies based on in vitro or in animal studies.

ACS Style

Vanessa Ribeiro; Nayra Dias; Taís Paiva; Luciana Hagström-Bex; Nadjar Nitz; Riccardo Pratesi; Mariana Hecht. Current trends in the pharmacological management of Chagas disease. International Journal for Parasitology: Drugs and Drug Resistance 2019, 12, 7 -17.

AMA Style

Vanessa Ribeiro, Nayra Dias, Taís Paiva, Luciana Hagström-Bex, Nadjar Nitz, Riccardo Pratesi, Mariana Hecht. Current trends in the pharmacological management of Chagas disease. International Journal for Parasitology: Drugs and Drug Resistance. 2019; 12 ():7-17.

Chicago/Turabian Style

Vanessa Ribeiro; Nayra Dias; Taís Paiva; Luciana Hagström-Bex; Nadjar Nitz; Riccardo Pratesi; Mariana Hecht. 2019. "Current trends in the pharmacological management of Chagas disease." International Journal for Parasitology: Drugs and Drug Resistance 12, no. : 7-17.

Validation study
Published: 27 September 2019 in Nutrients
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Celiac disease (CD) is an autoimmune disorder triggered by the ingestion of gluten and affects approximately 1% of the global population. Currently, the only treatment available is lifelong strict adherence to a gluten-free diet (GFD). Chronic diseases such as CD affect patients and their family members’ quality of life (QoL); particularly parents and caregivers who play an essential role in the child’s care and treatment. A higher level of psychological distress has been found in the parents of children with chronic ailments due to limited control over the child’s daily activities and the child’s illness. In this context, the validation of a specific questionnaire of QoL is a valuable tool to evaluate the difficulties faced by parents or caregivers of children with this chronic illness. A specific questionnaire for this population can elucidate the reasons for stress in their daily lives as well as the physical, mental, emotional, and social impact caused by CD. Therefore, this study aimed to develop and validate a specific questionnaire to evaluate the QoL of parents and caregivers of children and adolescents with CD. Overall results showed that a higher family income resulted in a higher score of the worries domain. In addition, having another illness besides CD decreased the QoL (except in the worries domain). The other variables studied did not present a statistically significant impact on the QoL, which was shown to be low in all aspects. Knowledge of the QoL is important to help implement effective strategies to improve celiac patients’ quality of life and reduce their physical, emotional, and social burden.

ACS Style

Liliane Maria Abreu Paiva; Lenora Gandolfi; Riccardo Pratesi; Rosa Harumi Uenishi; Renata Puppin Zandonadi; Eduardo Yoshio Nakano; Claudia B. Pratesi. Measuring Quality of Life in Parents or Caregivers of Children and Adolescents with Celiac Disease: Development and Content Validation of the Questionnaire. Nutrients 2019, 11, 2302 .

AMA Style

Liliane Maria Abreu Paiva, Lenora Gandolfi, Riccardo Pratesi, Rosa Harumi Uenishi, Renata Puppin Zandonadi, Eduardo Yoshio Nakano, Claudia B. Pratesi. Measuring Quality of Life in Parents or Caregivers of Children and Adolescents with Celiac Disease: Development and Content Validation of the Questionnaire. Nutrients. 2019; 11 (10):2302.

Chicago/Turabian Style

Liliane Maria Abreu Paiva; Lenora Gandolfi; Riccardo Pratesi; Rosa Harumi Uenishi; Renata Puppin Zandonadi; Eduardo Yoshio Nakano; Claudia B. Pratesi. 2019. "Measuring Quality of Life in Parents or Caregivers of Children and Adolescents with Celiac Disease: Development and Content Validation of the Questionnaire." Nutrients 11, no. 10: 2302.

Communication
Published: 16 August 2019 in Nutrients
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This study aimed to evaluate the occurrence of gluten contamination in naturally gluten-free meals from food services in the Federal District, Brazil. This is an exploratory cross-sectional quantitative study in which a total of 180 samples of naturally gluten-free dishes were collected from 60 food services in Brazil. The enzyme-linked immunosorbent assay was used for the quantification of gluten. As established by the Codex Alimentarius, the threshold of 20 ppm of gluten was considered as the accepted upper gluten level for gluten-free food. A total of 2.8% (95% CI: 0.3-5.2%) gluten contamination was found in the samples. Among the 60 food services, 6.7% (95% CI: 2.7-10.6%) displayed at least one contaminated food in our sample. The occurrence of gluten contamination in naturally gluten-free preparations was uncommon and low on a quantitative basis.

ACS Style

Priscila Farage; Renata Puppin Zandonadi; Lenora Gandolfi; Riccardo Pratesi; Ana Luísa Falcomer; Letícia Santos Araújo; Eduardo Yoshio Nakano; Verônica Cortez Ginani. Accidental Gluten Contamination in Traditional Lunch Meals from Food Services in Brasilia, Brazil. Nutrients 2019, 11, 1924 .

AMA Style

Priscila Farage, Renata Puppin Zandonadi, Lenora Gandolfi, Riccardo Pratesi, Ana Luísa Falcomer, Letícia Santos Araújo, Eduardo Yoshio Nakano, Verônica Cortez Ginani. Accidental Gluten Contamination in Traditional Lunch Meals from Food Services in Brasilia, Brazil. Nutrients. 2019; 11 (8):1924.

Chicago/Turabian Style

Priscila Farage; Renata Puppin Zandonadi; Lenora Gandolfi; Riccardo Pratesi; Ana Luísa Falcomer; Letícia Santos Araújo; Eduardo Yoshio Nakano; Verônica Cortez Ginani. 2019. "Accidental Gluten Contamination in Traditional Lunch Meals from Food Services in Brasilia, Brazil." Nutrients 11, no. 8: 1924.

Validation study
Published: 04 April 2019 in Nutrients
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This study aimed to translate, culturally adapt, validate, and apply a questionnaire to the Brazilian non-celiac gluten sensitive (NCGS) population. We also aimed to estimate the prevalence of symptoms which affect Brazilian NCGS. The Brazilian Portuguese version of the NCGS questionnaire was developed according to revised international guidelines. Five-hundred-and-fourty-three participants responded the NCGS questionnaire. We evaluated the reproducibility and validity of the questionnaire which presents valid measures of reproducibility. This is the first specific self-reported validated questionnaire for NCGS patients in Brazilian Portuguese, and the first nationwide characterization of self-reported NCGS in Brazilian adults. Most respondents were female (92.3%), and the main intestinal symptoms reported were bloating and abdominal pain. The most frequent extraintestinal symptoms were lack of wellbeing, tiredness, and depression. We expect that the present study will provide a picture of Brazilian individuals with suspected NCGS, which could help health professionals and governmental institutions in developing effective strategies to improve the treatment and diagnosis of Brazilian NCGS.

ACS Style

Yanna A. Gadelha De Mattos; Renata Puppin Zandonadi; Lenora Gandolfi; Riccardo Pratesi; Eduardo Yoshio Nakano; Claudia B. Pratesi. Self-Reported Non-Celiac Gluten Sensitivity in Brazil: Translation, Cultural Adaptation, and Validation of Italian Questionnaire. Nutrients 2019, 11, 781 .

AMA Style

Yanna A. Gadelha De Mattos, Renata Puppin Zandonadi, Lenora Gandolfi, Riccardo Pratesi, Eduardo Yoshio Nakano, Claudia B. Pratesi. Self-Reported Non-Celiac Gluten Sensitivity in Brazil: Translation, Cultural Adaptation, and Validation of Italian Questionnaire. Nutrients. 2019; 11 (4):781.

Chicago/Turabian Style

Yanna A. Gadelha De Mattos; Renata Puppin Zandonadi; Lenora Gandolfi; Riccardo Pratesi; Eduardo Yoshio Nakano; Claudia B. Pratesi. 2019. "Self-Reported Non-Celiac Gluten Sensitivity in Brazil: Translation, Cultural Adaptation, and Validation of Italian Questionnaire." Nutrients 11, no. 4: 781.

Evaluation study
Published: 10 September 2018 in Nutrients
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Gluten cross-contamination in gluten-free food may jeopardize treatment of celiac patients. Considering the deficit of appropriate instruments to enable the implementation of safe production practices for gluten-free food, this study aimed to evaluate the application of a check-list elaborated for gluten cross-contamination prevention in food services. The instrument was applied in 60 Brazilian food services. Interobserver reproducibility and internal consistency of the check-list were tested. A score classification was created for establishments according to the food contamination risk assessment. Subsequent to the application and statistical analysis, the original instrument was reduced to a 30-item check-list. In the reproducibility analysis, none of the 30 items showed significant divergence among the evaluators (p > 0.05 in the Cochran Q test). The 30-item version of the check-list presented Kuder–Richardson Formula 20 (KR-20) = 0.771, indicating good internal consistency. The proposed classification score is obtained by adding 1 point for each item with an “adequate” response; therefore, the final score may vary between 0 and 30 points. Establishments with up to 15 points exhibit risk of gluten contamination, while establishments with a score above 16 points exhibit low risk of contamination. The check-list displayed good reproducibility and internal consistency, suggesting that it could be a useful gluten contamination control instrument in food services.

ACS Style

Priscila Farage; Renata Puppin Zandonadi; Verônica Cortez Ginani; Lenora Gandolfi; Eduardo Yoshio Nakano; Riccardo Pratesi. Gluten-Free Diet: From Development to Assessment of a Check-List Designed for the Prevention of Gluten Cross-Contamination in Food Services. Nutrients 2018, 10, 1274 .

AMA Style

Priscila Farage, Renata Puppin Zandonadi, Verônica Cortez Ginani, Lenora Gandolfi, Eduardo Yoshio Nakano, Riccardo Pratesi. Gluten-Free Diet: From Development to Assessment of a Check-List Designed for the Prevention of Gluten Cross-Contamination in Food Services. Nutrients. 2018; 10 (9):1274.

Chicago/Turabian Style

Priscila Farage; Renata Puppin Zandonadi; Verônica Cortez Ginani; Lenora Gandolfi; Eduardo Yoshio Nakano; Riccardo Pratesi. 2018. "Gluten-Free Diet: From Development to Assessment of a Check-List Designed for the Prevention of Gluten Cross-Contamination in Food Services." Nutrients 10, no. 9: 1274.

Validation study
Published: 25 August 2018 in Nutrients
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(1) Background: Celiac disease (CD) is a common autoimmune disorder. The manifestations of the disease and the obligatory life-long gluten-free diet (GFD) are associated with the impairment of patients’ quality of life. Therefore, the present study aimed to translate, culturally adapt and validate a celiac disease quality of life (CD-QoL) questionnaire and apply it to a representative number of Brazilian CD patients. (2) Methods: A cross-cultural Brazilian-Portuguese version of the CD-QoL was developed according to revised international guidelines. The questionnaire was administered to 450 celiac patients. The reliability, reproducibility and validity were studied. (3) Results: The Brazilian CD-QoL questionnaire presents valid measures of reproducibility and internal consistency. Early diagnosis is related to higher scores of Brazilian CD-QoL social, sub- and total scale. There was a positive correlation between higher education level and higher QoL. Individuals with partners tend to have a better emotional subscale of QoL. CD-patients who follow a strict GFD have highest QoL scale values. Men scored higher than women on the CD-QoL. All results were statistically significant except for the gastrointestinal subscale. (4) Conclusions: Brazilian CD-QoL allows comparative research between different celiac populations in the world. QoL research will help in the development of effective strategies to improve Brazilian celiac patients’ quality of life.

ACS Style

Claudia B. Pratesi; Winfred Häuser; Rosa Harumi Uenishi; Nicole Selleski; Eduardo Yoshio Nakano; Lenora Gandolfi; Riccardo Pratesi; Renata Puppin Zandonadi. Quality of Life of Celiac Patients in Brazil: Questionnaire Translation, Cultural Adaptation and Validation. Nutrients 2018, 10, 1167 .

AMA Style

Claudia B. Pratesi, Winfred Häuser, Rosa Harumi Uenishi, Nicole Selleski, Eduardo Yoshio Nakano, Lenora Gandolfi, Riccardo Pratesi, Renata Puppin Zandonadi. Quality of Life of Celiac Patients in Brazil: Questionnaire Translation, Cultural Adaptation and Validation. Nutrients. 2018; 10 (9):1167.

Chicago/Turabian Style

Claudia B. Pratesi; Winfred Häuser; Rosa Harumi Uenishi; Nicole Selleski; Eduardo Yoshio Nakano; Lenora Gandolfi; Riccardo Pratesi; Renata Puppin Zandonadi. 2018. "Quality of Life of Celiac Patients in Brazil: Questionnaire Translation, Cultural Adaptation and Validation." Nutrients 10, no. 9: 1167.

Journal article
Published: 31 July 2018 in Case Reports in Perinatal Medicine
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Kawasaki disease (KD) is one of the most common causes of childhood systemic vasculitis, with the potential to cause significant cardiovascular complications, especially if undiagnosed. An investigation of coronary artery abnormalities with the application of appropriate therapeutic measures can improve the prognosis in these infants, decreasing the risk of coronary artery aneurysms. Neonatal KD is a rare event, often characterized only by few of the clinical features observed in older children. In this case report, we describe a newborn male who presented an incomplete clinical presentation of KD, where symptoms of the disease erupted immediately after birth. The objective of this report is to draw the attention of pediatricians and neonatologists to the possible presence of KD in an asymptomatic newborn during the immediate postnatal period, even in the absence of the classic features of KD, such as fever.

ACS Style

Cristina Medeiros Ribeiro De Magalhães; Riccardo Pratesi; Lenora Gandolfi; Rosa Harume Uenishi; Claudia B. Pratesi. Onset of Kawasaki disease immediately after birth. Case Reports in Perinatal Medicine 2018, 7, 1 .

AMA Style

Cristina Medeiros Ribeiro De Magalhães, Riccardo Pratesi, Lenora Gandolfi, Rosa Harume Uenishi, Claudia B. Pratesi. Onset of Kawasaki disease immediately after birth. Case Reports in Perinatal Medicine. 2018; 7 (2):1.

Chicago/Turabian Style

Cristina Medeiros Ribeiro De Magalhães; Riccardo Pratesi; Lenora Gandolfi; Rosa Harume Uenishi; Claudia B. Pratesi. 2018. "Onset of Kawasaki disease immediately after birth." Case Reports in Perinatal Medicine 7, no. 2: 1.

Research article
Published: 11 June 2018 in BMC Complementary and Alternative Medicine
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Anacardium occidentale L phenolic lipid (LDT11) is used in traditional medicine as anti-inflammatory, astringent, antidiarrheal, anti-asthmatic and depurative. Phenolic derivatives, such as anacardic acid, extracted from cashew nut shell liquid (CNSL) have demonstrated biological and pharmacological properties, and its profile makes it a candidate for the development of new anti-inflammatory agents. The objective of the present study was to evaluate the anti-inflammatory profile of a derivative, synthesized from LDT11, on an in vitro cellular model. Organic synthesis of the phenolic derivative of CNSL that results in the hemi-synthetic compound LDT11. The cytotoxicity of the planned compound, LDT11, was analyzed in murine macrophages cell line, RAW264.7. The cells were previously treated with LDT11, and then, the inflammation was stimulated with lipopolysaccharide (LPS), in intervals of 6 h and 24 h. The analysis of the gene expression of inflammatory markers (TNFα, iNOS, COX-2, NF-κB, IL-1β and IL-6), nitric oxide (NO) dosage, and cytokine IL-6 were realized. The results showed that the phenolic derivative, LDT11, influenced the modulatory gene expression. The relative gene transcripts quantification demonstrated that the LDT11 disclosed an immunoprotective effect against inflammation by decreasing genes expression when compared with cells stimulated with LPS in the control group. The NO and IL-6 dosages confirmed the results found in gene expression. The present study evaluated the immunoprotective effect of LDT11. In addition to a significant reduction in the expression of inflammatory genes, LDT11 also had a faster and superior anti-inflammatory action than the commercial products, and its response was already evident in the test carried out six hours after the treatment of the cells. This study demonstrated LDT11 is potentially valuable as a rapid immunoprotective anti-inflammatory agent. Treatment with LDT11 decreased the gene expression of inflammatory markers, and the NO, and IL-6 production. When compared to commercial drugs, LDT11 showed a superior anti-inflammatory action.

ACS Style

Marilen Queiroz de Souza; Isabella Márcia Soares Nogueira Teotônio; Fernanda Coutinho de Almeida; Gabriella Simões Heyn; Priscilla Souza Alves; Luiz Antônio Soares Romeiro; Riccardo Pratesi; Yanna Karla De Medeiros Nóbrega; Claudia B. Pratesi. Molecular evaluation of anti-inflammatory activity of phenolic lipid extracted from cashew nut shell liquid (CNSL). BMC Complementary and Alternative Medicine 2018, 18, 181 .

AMA Style

Marilen Queiroz de Souza, Isabella Márcia Soares Nogueira Teotônio, Fernanda Coutinho de Almeida, Gabriella Simões Heyn, Priscilla Souza Alves, Luiz Antônio Soares Romeiro, Riccardo Pratesi, Yanna Karla De Medeiros Nóbrega, Claudia B. Pratesi. Molecular evaluation of anti-inflammatory activity of phenolic lipid extracted from cashew nut shell liquid (CNSL). BMC Complementary and Alternative Medicine. 2018; 18 (1):181.

Chicago/Turabian Style

Marilen Queiroz de Souza; Isabella Márcia Soares Nogueira Teotônio; Fernanda Coutinho de Almeida; Gabriella Simões Heyn; Priscilla Souza Alves; Luiz Antônio Soares Romeiro; Riccardo Pratesi; Yanna Karla De Medeiros Nóbrega; Claudia B. Pratesi. 2018. "Molecular evaluation of anti-inflammatory activity of phenolic lipid extracted from cashew nut shell liquid (CNSL)." BMC Complementary and Alternative Medicine 18, no. 1: 181.

Brief report
Published: 01 November 2017 in Viral Immunology
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Rubella is an acute viral disease that usually does not generate sequels; however, in pregnant women the infection can cause serious abnormalities to fetuses, which are collectively called congenital rubella syndrome. In Brazil, population immunization was started in 1992, but few epidemiological studies have been conducted to assess vaccination coverage and seroconversion since then. The aim of this work is to evaluate the seropositivity of pregnant women to rubella virus after vaccination campaign was carried out in 2008. Serological tests for rubella diagnosis were performed in 87 pregnant women who attended the University of Brasilia Hospital, Federal District, Brazil. Antirubella IgG antibodies were detected in 83 out of 87 pregnant women (95.4%), with an age-independent seroprevalence. Only one woman was positive in IgM serological tests. Our data suggest high levels of vaccination coverage and antirubella immunization in the Brazil Federal District population.

ACS Style

Yanna K.M. Nóbrega; Bruna Caroline de Carvalho; Nadjar Nitz; Tamires E. Vital; Franco B. Leite; Inês Jorge Sequeira; Elsa Moreira; Juliana K.B. De Andrade; Lenora Gandolfi; Riccardo Pratesi; Mariana M. Hecht. Rubella Seropositivity in Pregnant Women After Vaccination Campaign in Brazil's Federal District. Viral Immunology 2017, 30, 675 -677.

AMA Style

Yanna K.M. Nóbrega, Bruna Caroline de Carvalho, Nadjar Nitz, Tamires E. Vital, Franco B. Leite, Inês Jorge Sequeira, Elsa Moreira, Juliana K.B. De Andrade, Lenora Gandolfi, Riccardo Pratesi, Mariana M. Hecht. Rubella Seropositivity in Pregnant Women After Vaccination Campaign in Brazil's Federal District. Viral Immunology. 2017; 30 (9):675-677.

Chicago/Turabian Style

Yanna K.M. Nóbrega; Bruna Caroline de Carvalho; Nadjar Nitz; Tamires E. Vital; Franco B. Leite; Inês Jorge Sequeira; Elsa Moreira; Juliana K.B. De Andrade; Lenora Gandolfi; Riccardo Pratesi; Mariana M. Hecht. 2017. "Rubella Seropositivity in Pregnant Women After Vaccination Campaign in Brazil's Federal District." Viral Immunology 30, no. 9: 675-677.

Original articles
Published: 26 June 2017 in Archives of Endocrinology and Metabolism
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Objective The purpose of this study was to verify the presence of endothelial dysfunction and initial structural atherosclerotic changes in children with Type 1 diabetes mellitus (T1DM). Subjects and methods The study population comprised 31 diabetic children aged 6 to 12 years, divided into two subgroups according to the duration of the T1DM diagnosis: subgroup 1, with less than 5 years elapsed since diagnosis, and subgroup 2, with more than 5 years elapsed since diagnosis. The control group comprised 58 age-matched healthy children. Ultrasonographic techniques were used to measure the flow-mediated dilatation (FMD) of the brachial artery and the intima-media thickness (IMT) of the carotid arteries. Results Children with T1DM with longer disease duration showed significantly decreased mean values of FMD compared with those in the control group. No significant differences between the groups were found in relation to IMT. The FMD percentage presented a moderate negative correlation with glycated hemoglobin (HbA1c) and fasting glucose levels. Conclusion Our findings suggest that endothelial dysfunction may be already present in children with 5 years or more elapsed since diagnosis, even in the absence of atherosclerotic structural changes. The decreased vasodilation response correlated with hyperglycemia. Key words: Type 1 diabetes mellitus; endothelial dysfunction; atherosclerosis; flow-mediated dilation; carotid intima-media thickness

ACS Style

Antonella Márcia Mercadante De Albuquerque Do Nascimento; Inês Jorge Sequeira; Daniel França Vasconcelos; Lenora Gandolfi; Yanna Karla De Medeiros Nóbrega; Riccardo Pratesi. Endothelial dysfunction in children with type 1 diabetes mellitus. Archives of Endocrinology and Metabolism 2017, 61, 476 -483.

AMA Style

Antonella Márcia Mercadante De Albuquerque Do Nascimento, Inês Jorge Sequeira, Daniel França Vasconcelos, Lenora Gandolfi, Yanna Karla De Medeiros Nóbrega, Riccardo Pratesi. Endothelial dysfunction in children with type 1 diabetes mellitus. Archives of Endocrinology and Metabolism. 2017; 61 (5):476-483.

Chicago/Turabian Style

Antonella Márcia Mercadante De Albuquerque Do Nascimento; Inês Jorge Sequeira; Daniel França Vasconcelos; Lenora Gandolfi; Yanna Karla De Medeiros Nóbrega; Riccardo Pratesi. 2017. "Endothelial dysfunction in children with type 1 diabetes mellitus." Archives of Endocrinology and Metabolism 61, no. 5: 476-483.

Validation study
Published: 06 January 2017 in Nutrients
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Conditions associated to the consumption of gluten have emerged as a major health care concern and the treatment consists on a lifelong gluten-free diet. Providing safe food for these individuals includes adapting to safety procedures within the food chain and preventing gluten cross-contamination in gluten-free food. However, a gluten cross-contamination prevention protocol or check-list has not yet been validated. Therefore, the aim of this study was to perform the content validation and semantic evaluation of a check-list elaborated for the prevention of gluten cross-contamination in food services. The preliminary version of the check-list was elaborated based on the Brazilian resolution for food safety Collegiate Board Resolution 216 (RDC 216) and Collegiate Board Resolution 275 (RDC 275), the standard 22000 from the International Organization for Standardization (ISO 22000) and the Canadian Celiac Association Gluten-Free Certification Program documents. Seven experts with experience in the area participated in the check-list validation and semantic evaluation. The criteria used for the approval of the items, as to their importance for the prevention of gluten cross-contamination and clarity of the wording, was the achievement of a minimal of 80% of agreement between the experts (W-values ≥ 0.8). Moreover, items should have a mean ≥4 in the evaluation of importance (Likert scale from 1 to 5) and clarity (Likert scale from 0 to 5) in order to be maintained in the instrument. The final version of the check-list was composed of 84 items, divided into 12 sections. After being redesigned and re-evaluated, the items were considered important and comprehensive by the experts (both with W-values ≥ 0.89). The check-list developed was validated with respect to content and approved in the semantic evaluation.

ACS Style

Priscila Farage; Renata Puppin Zandonadi; Verônica Cortez Ginani; Lenora Gandolfi; Riccardo Pratesi; Yanna Karla De Medeiros Nóbrega. Content Validation and Semantic Evaluation of a Check-List Elaborated for the Prevention of Gluten Cross-Contamination in Food Services. Nutrients 2017, 9, 36 .

AMA Style

Priscila Farage, Renata Puppin Zandonadi, Verônica Cortez Ginani, Lenora Gandolfi, Riccardo Pratesi, Yanna Karla De Medeiros Nóbrega. Content Validation and Semantic Evaluation of a Check-List Elaborated for the Prevention of Gluten Cross-Contamination in Food Services. Nutrients. 2017; 9 (1):36.

Chicago/Turabian Style

Priscila Farage; Renata Puppin Zandonadi; Verônica Cortez Ginani; Lenora Gandolfi; Riccardo Pratesi; Yanna Karla De Medeiros Nóbrega. 2017. "Content Validation and Semantic Evaluation of a Check-List Elaborated for the Prevention of Gluten Cross-Contamination in Food Services." Nutrients 9, no. 1: 36.

Short communications
Published: 01 October 2016 in Revista da Sociedade Brasileira de Medicina Tropical
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Key words: Toxocara spp; Pregnancy; Animal contact

ACS Style

Lívia Custódio Pereira; Guita Rubinsky Elefant; Yanna Medeiros Nóbrega; Tamires Vital; Nadjar Nitz; Lenora Gandolfi; Riccardo Pratesi; Mariana Hecht. Toxocara spp. seroprevalence in pregnant women in Brasília, Brazil. Revista da Sociedade Brasileira de Medicina Tropical 2016, 49, 641 -643.

AMA Style

Lívia Custódio Pereira, Guita Rubinsky Elefant, Yanna Medeiros Nóbrega, Tamires Vital, Nadjar Nitz, Lenora Gandolfi, Riccardo Pratesi, Mariana Hecht. Toxocara spp. seroprevalence in pregnant women in Brasília, Brazil. Revista da Sociedade Brasileira de Medicina Tropical. 2016; 49 (5):641-643.

Chicago/Turabian Style

Lívia Custódio Pereira; Guita Rubinsky Elefant; Yanna Medeiros Nóbrega; Tamires Vital; Nadjar Nitz; Lenora Gandolfi; Riccardo Pratesi; Mariana Hecht. 2016. "Toxocara spp. seroprevalence in pregnant women in Brasília, Brazil." Revista da Sociedade Brasileira de Medicina Tropical 49, no. 5: 641-643.

Journal article
Published: 01 September 2016 in Multiple Sclerosis and Related Disorders
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Comorbidity of celiac disease with demyelinating diseases of the central nervous system has been reported since the 1960s. The objective of this study was to determine the serological prevalence of celiac disease in the largest series of patients diagnosed with multiple sclerosis, neuromyelitis optica, or myelitis.

ACS Style

Pérola De Oliveira; Daniel Rocha De Carvalho; Ivar Viana Brandi; Riccardo Pratesi. Serological prevalence of celiac disease in Brazilian population of multiple sclerosis, neuromyelitis optica and myelitis. Multiple Sclerosis and Related Disorders 2016, 9, 125 -128.

AMA Style

Pérola De Oliveira, Daniel Rocha De Carvalho, Ivar Viana Brandi, Riccardo Pratesi. Serological prevalence of celiac disease in Brazilian population of multiple sclerosis, neuromyelitis optica and myelitis. Multiple Sclerosis and Related Disorders. 2016; 9 ():125-128.

Chicago/Turabian Style

Pérola De Oliveira; Daniel Rocha De Carvalho; Ivar Viana Brandi; Riccardo Pratesi. 2016. "Serological prevalence of celiac disease in Brazilian population of multiple sclerosis, neuromyelitis optica and myelitis." Multiple Sclerosis and Related Disorders 9, no. : 125-128.

Original articles
Published: 01 June 2015 in Arquivos de Gastroenterologia
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Background Celiac disease is an autoimmune enteropathy triggered by the ingestion of gluten in genetically susceptible individuals. Genetic susceptibility is associated with two sets of alleles, DQA1*05 - DQB1*02 and DQA1*03 - DQB1*03:02, which code for class II MHC DQ2 and DQ8 molecules, respectively. Approximately 90%-95% of celiac patients are HLA-DQ2 positive, and half of the remaining patients are HLA-DQ8 positive. In fact, during a celiac disease diagnostic workup, the absence of these specific DQA and DQB alleles has a near perfect negative predictive value. Objective Improve the detection of celiac disease predisposing alleles by combining the simplicity and sensitivity of real-time PCR (qPCR) and melting curve analysis with the specificity of sequence-specific primers (SSP). Methods Amplifications of sequence-specific primers for DQA1*05 (DQ2), DQB1*02 (DQ2), and DQA1*03 (DQ8) were performed by the real time PCR method to determine the presence of each allele in independent reactions. Primers for Human Growth Hormone were used as an internal control. A parallel PCR-SSP protocol was used as a reference method to validate our results. Results Both techniques yielded equal results. From a total of 329 samples the presence of HLA predisposing alleles was determined in 187 (56.8%). One hundred fourteen samples (61%) were positive for a single allele, 68 (36.3%) for two alleles, and only 5 (2.7%) for three alleles. Conclusion Results obtained by qPCR technique were highly reliable with no discordant results when compared with those obtained using PCR-SSP.

ACS Style

Nicole Selleski; Lucas Malta Almeida; Fernanda Coutinho De Almeida; Lenora Gandolfi; Riccardo Pratesi; Yanna Karla De Medeiros Nóbrega. SIMPLIFYING CELIAC DISEASE PREDISPOSING HLA-DQ ALLELES DETERMINATION BY THE REAL TIME PCR METHOD. Arquivos de Gastroenterologia 2015, 52, 143 -146.

AMA Style

Nicole Selleski, Lucas Malta Almeida, Fernanda Coutinho De Almeida, Lenora Gandolfi, Riccardo Pratesi, Yanna Karla De Medeiros Nóbrega. SIMPLIFYING CELIAC DISEASE PREDISPOSING HLA-DQ ALLELES DETERMINATION BY THE REAL TIME PCR METHOD. Arquivos de Gastroenterologia. 2015; 52 (2):143-146.

Chicago/Turabian Style

Nicole Selleski; Lucas Malta Almeida; Fernanda Coutinho De Almeida; Lenora Gandolfi; Riccardo Pratesi; Yanna Karla De Medeiros Nóbrega. 2015. "SIMPLIFYING CELIAC DISEASE PREDISPOSING HLA-DQ ALLELES DETERMINATION BY THE REAL TIME PCR METHOD." Arquivos de Gastroenterologia 52, no. 2: 143-146.