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Solanum nigrum L., a hyperaccumulator of cadmium (Cd), is regarded as a promising candidate for phytoremediation of heavy metal pollution. In the present study, the hairy roots of Solanum nigrum L. were selected as a model plant system to study the potential application of Iron-regulated Transporter Gene (IRT1) for the efficient phytoremediation of Cd pollution. The transgenic hairy roots of Solanum nigrum L. expressing the IRT1 gene from Arabidopsis thaliana were successfully obtained via the Agrobacterium tumegaciens-mediated method. Expression of IRT1 reduced Cd stress-induced phytotoxic effects. Significantly superior root growth, increased antioxidant enzyme activities, decreased reactive oxygen species (ROS) levels, and less cell apoptosis were observed in the transgenic hairy roots of Solanum nigrum L. compared to the wild-type lines under Cd stress. Enhanced Cd accumulation was also carried out in the transgenic hairy roots compared to the control (886.8 μg/g vs. 745.0 μg/g). These results provide an important understanding of the Cd tolerance mechanism of transgenic IRT1 hairy roots of Solanum nigrum L., and are of particular importance to the development of a transgenic candidate for efficient phytoremediation process.
Peng Ye; Menghua Wang; Teng Zhang; Xiaoyu Liu; He Jiang; Yaping Sun; Xiyu Cheng; Qiong Yan. Enhanced Cadmium Accumulation and Tolerance in Transgenic Hairy Roots of Solanum nigrum L. Expressing Iron-Regulated Transporter Gene IRT1. Life 2020, 10, 324 .
AMA StylePeng Ye, Menghua Wang, Teng Zhang, Xiaoyu Liu, He Jiang, Yaping Sun, Xiyu Cheng, Qiong Yan. Enhanced Cadmium Accumulation and Tolerance in Transgenic Hairy Roots of Solanum nigrum L. Expressing Iron-Regulated Transporter Gene IRT1. Life. 2020; 10 (12):324.
Chicago/Turabian StylePeng Ye; Menghua Wang; Teng Zhang; Xiaoyu Liu; He Jiang; Yaping Sun; Xiyu Cheng; Qiong Yan. 2020. "Enhanced Cadmium Accumulation and Tolerance in Transgenic Hairy Roots of Solanum nigrum L. Expressing Iron-Regulated Transporter Gene IRT1." Life 10, no. 12: 324.
A two-step process was proposed to enhance enzymatic hydrolysis of Miscanthus sinensis based on a comparative study of acid/alkaline pretreatments. Ferric chloride pretreatment (FP) effectively removed hemicellulose and recovered soluble sugars, but the enzymatic hydrolysis was not efficient. Dilute alkaline pretreatment (ALP) resulted in much better delignification and stronger morphological changes of the sample, making it more accessible to enzymes. While ALP obtained the highest sugar yield during enzymatic hydrolysis, the soluble sugar recovery from the pretreatment stage was still limited. Furthermore, a two-step ferric chloride and dilute alkaline pretreatment (F-ALP) has been successfully developed by effectively recovering soluble sugars in the first FP step and further removing lignin of the FP sample in the second ALP step to improve its enzymatic hydrolysis. As a result, the two-step process yielded the highest total sugar recovery (418.8 mg/g raw stalk) through the whole process.
Lingci Li; Peng Ye; Mengyu Chen; Shangyuan Tang; Ying Luo; Yifan Gao; Qiong Yan; Xiyu Cheng. A Two-Step Ferric Chloride and Dilute Alkaline Pretreatment for Enhancing Enzymatic Hydrolysis and Fermentable Sugar Recovery from Miscanthus sinensis. Molecules 2020, 25, 1843 .
AMA StyleLingci Li, Peng Ye, Mengyu Chen, Shangyuan Tang, Ying Luo, Yifan Gao, Qiong Yan, Xiyu Cheng. A Two-Step Ferric Chloride and Dilute Alkaline Pretreatment for Enhancing Enzymatic Hydrolysis and Fermentable Sugar Recovery from Miscanthus sinensis. Molecules. 2020; 25 (8):1843.
Chicago/Turabian StyleLingci Li; Peng Ye; Mengyu Chen; Shangyuan Tang; Ying Luo; Yifan Gao; Qiong Yan; Xiyu Cheng. 2020. "A Two-Step Ferric Chloride and Dilute Alkaline Pretreatment for Enhancing Enzymatic Hydrolysis and Fermentable Sugar Recovery from Miscanthus sinensis." Molecules 25, no. 8: 1843.
Energy crops are not easily converted by microorganisms because of their recalcitrance. This necessitates a pretreatment to improve their biodigestibility. The effects of different pretreatments, as well as their combination on the enzymatic digestibility of Arundo donax L. were systematically investigated to evaluate its potential for bioconversion. Dilute alkaline pretreatment (ALP) using 1.2% NaOH at 120 °C for 30 min resulted in the highest reducing sugar yield in the enzymatic hydrolysis process because of its strong delignification and morphological modification, while ferric chloride pretreatment (FP) was effective in removing hemicellulose and recovering soluble sugars in the pretreatment stage. Furthermore, an efficient two-step ferric chloride-alkaline pretreatment (FALP) was successfully developed. In the first FP step, easily degradable cellulosic components, especially hemicellulose, were dissolved and then effectively recovered as soluble sugars. Subsequently, the FP sample was further treated in the second ALP step to remove lignin to enhance the enzymatic hydrolysis of the hardly degradable cellulose. As a result, the integrated two-step process obtained the highest total sugar yield of 420.4 mg/g raw stalk in the whole pretreatment and enzymatic hydrolysis process; hence, the process is a valuable candidate for biofuel production.
Shangyuan Tang; Yushen Cao; Chunming Xu; Yue Wu; Lingci Li; Peng Ye; Ying Luo; Yifan Gao; Yonghong Liao; Qiong Yan; Xiyu Cheng. One-Step or Two-Step Acid/Alkaline Pretreatments to Improve Enzymatic Hydrolysis and Sugar Recovery from Arundo Donax L. Energies 2020, 13, 948 .
AMA StyleShangyuan Tang, Yushen Cao, Chunming Xu, Yue Wu, Lingci Li, Peng Ye, Ying Luo, Yifan Gao, Yonghong Liao, Qiong Yan, Xiyu Cheng. One-Step or Two-Step Acid/Alkaline Pretreatments to Improve Enzymatic Hydrolysis and Sugar Recovery from Arundo Donax L. Energies. 2020; 13 (4):948.
Chicago/Turabian StyleShangyuan Tang; Yushen Cao; Chunming Xu; Yue Wu; Lingci Li; Peng Ye; Ying Luo; Yifan Gao; Yonghong Liao; Qiong Yan; Xiyu Cheng. 2020. "One-Step or Two-Step Acid/Alkaline Pretreatments to Improve Enzymatic Hydrolysis and Sugar Recovery from Arundo Donax L." Energies 13, no. 4: 948.
Brassica campestris L., a hyperaccumulator of cadmium (Cd), is considered a candidate plant for efficient phytoremediation. The hairy roots of Brassica campestris L are chosen here as a model plant system to investigate the response mechanism of Brassica campestris L. to Cd stress. High-throughput sequencing technology is used to identify genes related to Cd tolerance. A total of 2394 differentially expressed genes (DEGs) are identified by RNA-Seq analysis, among which 1564 genes are up-regulated, and 830 genes are down-regulated. Data from the gene ontology (GO) analysis indicate that DEGs are mainly involved in metabolic processes. Glutathione metabolism, in which glutathione synthetase and glutathione S-transferase are closely related to Cd stress, is identified in the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. A Western blot shows that glutathione synthetase and glutathione S-transferase are involved in Cd tolerance. These results provide a preliminary understanding of the Cd tolerance mechanism of Brassica campestris L. and are, hence, of particular importance to the future development of an efficient phytoremediation process based on hairy root cultures, genetic modification, and the subsequent regeneration of the whole plant.
Yaping Sun; Qianyun Lu; Yushen Cao; Menghua Wang; Xiyu Cheng; Qiong Yan. Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress. International Journal of Molecular Sciences 2019, 21, 180 .
AMA StyleYaping Sun, Qianyun Lu, Yushen Cao, Menghua Wang, Xiyu Cheng, Qiong Yan. Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress. International Journal of Molecular Sciences. 2019; 21 (1):180.
Chicago/Turabian StyleYaping Sun; Qianyun Lu; Yushen Cao; Menghua Wang; Xiyu Cheng; Qiong Yan. 2019. "Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress." International Journal of Molecular Sciences 21, no. 1: 180.
In this study, effects of different pretreatment methods on the enzymatic digestibility of Pennisetum alopecuroides, a ubiquitous wild grass in China, were investigated to evaluate its potential as a feedstock for biofuel production. The stalk samples were separately pretreated with H2SO4, NaOH and FeCl3 solutions of different concentrations at 120 °C for 30 min, after which enzymatic hydrolysis was conducted to measure the digestibility of pretreated samples. Results demonstrated that different pretreatments were effective at removing hemicellulose, among which ferric chloride pretreatment (FCP) gave the highest soluble sugar recovery (200.2 mg/g raw stalk) from the pretreatment stage. In comparison with FCP and dilute acid pretreatment (DAP), dilute alkaline pretreatment (DALP) induced much higher delignification and stronger morphological changes of the biomass, making it more accessible to hydrolysis enzymes. As a result, DALP using 1.2% NaOH showed the highest total soluble sugar yield through the whole process from pretreatment to enzymatic hydrolysis (508.5 mg/g raw stalk). The present work indicates that DALP and FCP have the potential to enhance the effective bioconversion of lignocellulosic biomass like P. alopecuroides, hence making this material a valuable and promising energy plant.
Shangyuan Tang; Chunming Xu; Linh Tran Khanh Vu; Sicheng Liu; Peng Ye; Lingci Li; Yuxuan Wu; Mengyu Chen; Yao Xiao; Yue Wu; Yining Wang; Qiong Yan; Xiyu Cheng. Enhanced Enzymatic Hydrolysis of Pennisetum alopecuroides by Dilute Acid, Alkaline and Ferric Chloride Pretreatments. Molecules 2019, 24, 1715 .
AMA StyleShangyuan Tang, Chunming Xu, Linh Tran Khanh Vu, Sicheng Liu, Peng Ye, Lingci Li, Yuxuan Wu, Mengyu Chen, Yao Xiao, Yue Wu, Yining Wang, Qiong Yan, Xiyu Cheng. Enhanced Enzymatic Hydrolysis of Pennisetum alopecuroides by Dilute Acid, Alkaline and Ferric Chloride Pretreatments. Molecules. 2019; 24 (9):1715.
Chicago/Turabian StyleShangyuan Tang; Chunming Xu; Linh Tran Khanh Vu; Sicheng Liu; Peng Ye; Lingci Li; Yuxuan Wu; Mengyu Chen; Yao Xiao; Yue Wu; Yining Wang; Qiong Yan; Xiyu Cheng. 2019. "Enhanced Enzymatic Hydrolysis of Pennisetum alopecuroides by Dilute Acid, Alkaline and Ferric Chloride Pretreatments." Molecules 24, no. 9: 1715.
Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and adsorption was studied. The simulated results showed that enhancement of tanshinone production was mainly due to the effect of the elicitor and that resin addition resulted in adsorbance of the tanshinones from the root and alteration of tanshinone distribution. Furthermore, parameter sensitivities analysis showed that the rate of transport of tanshinones from the root to the medium was the important factor that influenced tanshinone accumulation in the resin. In conclusion, the modeling of tanshinone synthesis and phase distribution identified the process mechanism under the combined effect of elicitation and adsorption and this modeling can be used in similar plant tissue culture systems.
Qiong Yan; Jian-Yong Wu; Rui Liu. Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and in situ adsorption in Salvia miltiorrhiza hairy root cultures. Biotechnology Letters 2010, 33, 813 -819.
AMA StyleQiong Yan, Jian-Yong Wu, Rui Liu. Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and in situ adsorption in Salvia miltiorrhiza hairy root cultures. Biotechnology Letters. 2010; 33 (4):813-819.
Chicago/Turabian StyleQiong Yan; Jian-Yong Wu; Rui Liu. 2010. "Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and in situ adsorption in Salvia miltiorrhiza hairy root cultures." Biotechnology Letters 33, no. 4: 813-819.
Ag+ was employed as an abiotic elicitor to stimulate the secondary metabolite production in hairy root culture of Salvia miltiorrhiza, a valuable herbal plant. The addition of Ag2S2O3 at 15 - 40 μM to the culture between 12 and 22 days post inoculation resulted in more than 2-fold increase in the yields of the three diterpenoid tanshinones, tanshinone I, tanshinone IIA and cryptotanshinone. This stimulating effect was dependent on the Ag+ dose, the day of Ag+ addition to the culture and the tanshinone species, while the total yield of the three tanshinones (TT) was mainly dependent on the Ag+ dose, with the highest being attained at 30 μM. The Ag+ treatment also caused a dose-dependent inhibition of hairy root growth. Sucrose feeding or medium renewal before the addition of Ag+ to the culture effectively prevented the growth inhibition, and significantly increased the biomass concentration and volumetric tanshinone yield. With combined medium renewal and Ag+ treatment, in particular, the TT yield was increased 6.6-fold relative to that of the control (55.7 mg/L versus 7.3 mg/L). The elicitor function of Ag+ in the hairy root culture was validated by its ability to induce the characteristic elicitor responses of plants, the increase in cross-cell membrane ion fluxes and the production of reactive oxygen species.
Changhe Zhang; Qiong Yan; Wai-Keung Cheuk; Jian-Yong Wu. Enhancement of Tanshinone Production inSalvia miltiorrhizaHairy Root Culture by Ag+Elicitation and Nutrient Feeding. Planta Medica 2004, 70, 147 -151.
AMA StyleChanghe Zhang, Qiong Yan, Wai-Keung Cheuk, Jian-Yong Wu. Enhancement of Tanshinone Production inSalvia miltiorrhizaHairy Root Culture by Ag+Elicitation and Nutrient Feeding. Planta Medica. 2004; 70 (2):147-151.
Chicago/Turabian StyleChanghe Zhang; Qiong Yan; Wai-Keung Cheuk; Jian-Yong Wu. 2004. "Enhancement of Tanshinone Production inSalvia miltiorrhizaHairy Root Culture by Ag+Elicitation and Nutrient Feeding." Planta Medica 70, no. 2: 147-151.