Inflammation and angiogenesis are key facets of cardiovascular disease pathophysiology. Age and physical activity level can influence fasting systemic inflammation, but the impact of these factors on postprandial inflammation is unknown. In addition, markers of angiogenesis have never been tested in the context of a single high-fat meal (HFM). The purpose of this study was to investigate the effects of an HFM on markers of inflammation and angiogenesis in individuals of different ages and physical activity levels. Twenty-two healthy adults—8 younger active (YA) adults (4 men, 4 women; mean ± SD age: 25 ± 5 y), 8 older active (OA) adults (4 men, 4 women; 67 ± 5 y), and 6 older inactive (OI) adults (3 men, 3 women; 68 ± 7 y)—consumed an HFM [63% fat (39% saturated fat, 14% monounsaturated fat, 10% polyunsaturated fat), 34% carbohydrate; 12 kcal/kg body mass; 927 ± 154 kcal]. Fourteen inflammatory and 9 angiogenic markers were measured at baseline and 3 and 6 h postmeal. Significant group effects were observed in interleukin (IL)-10 (YA > OA; P = 0.02), IL-23 (YA > OA; P = 0.02), tumor necrosis factor (TNF)-α (OA < OI; P = 0.04), and vascular endothelial growth factor (VEGF)-C (YA < OA; P = 0.001). IL-8, VEGF-A, VEGF-C, and heparin-binding epidermal growth factor–like growth factor significantly increased, whereas granulocyte-macrophage colony-stimulating factor, interferon-γ, IL-1β, IL-5, IL-10, IL-12, IL-13, IL-17A, IL-23, TNF-α, leptin, angiopoietin-2, and follistatin significantly decreased after HFM consumption (P’s < 0.05). Notably, VEGF-A and VEGF-C were significantly higher at 3 h [mean difference: 22.5 pg/mL (VEGF-A); 73.5 pg/mL (VEGF-C)] and 6 h postmeal [mean difference: 26.9 pg/mL (VEGF-A); 81.2 pg/mL (VEGF-C)]. A novel finding of this study was the robust increase in VEGF after an HFM. There were also group differences in several inflammatory markers (IL-10 and IL-23 greater in YA than OA, and TNF-α lower in OA than OI) that suggest a potential influence of age and physical activity level.

Background: Postprandial lipemia (PPL) is a cardiovascular disease risk factor. However, the effects of different fat sources on PPL remain unclear. We aimed to determine the postprandial response in triglycerides (TG) to four dietary fat sources in adults. Methods: Participants completed four randomized meal trials. For each meal trial, participants (n = 10; 5M/5F) consumed a high-fat meal (HFM) (13 kcal/kg; 61% of total kcal from fat) with the fat source derived from butter, coconut oil, olive oil, or canola oil. Blood was drawn hourly for 6 h post-meal to quantify PPL. Results: Two-way ANOVA of TG revealed a time effect (p < 0.0001), but no time–meal interaction (p = 0.56), or meal effect (p = 0.35). Meal trials did not differ with regard to TG total (p = 0.33) or incremental (p = 0.14) area-under-the-curve. When stratified by sex and the TG response was averaged across meals, two-way ANOVA revealed a time effect (p < 0.0001), time–group interaction (p = 0.0001), and group effect (p = 0.048), with men exhibiting a greater response than women, although this difference could be attributed to the pronounced difference in BMI between men and women within the sample. Conclusion: In our sample of young adults, postprandial TG responses to a single HFM comprised of different fat sources did not differ.

Background: A large post-meal triglyceride (TG) response is an independent risk factor for cardiovascular disease, but postprandial lipemia assessments are not clinically practical in their current form. Therefore, we assessed the validity of an abbreviated, clinically feasible protocol in measuring postprandial lipemia. Method: Eighteen healthy adults (8 male and 10 female) completed 3 high-fat meal trials in random order: (1) a Standard in Lab (SL) protocol wherein blood draws (to determine TG) were made from a catheter at baseline and hourly for 6 h; (2) an Abbreviated in Lab (AL) protocol in which participants remained in the laboratory but blood draws were only made at baseline and 4 h post-meal; and (3) an Abbreviated with Freedom (AF) protocol in which participants vacated the laboratory between the meal and the 4-h blood draw. Results: TG increase from baseline was very similar (p = 0.93) across the 3 trials (SL: 68.5 ± 62.7 mg/dL; AL: 71.1 ± 58.0 mg/dL; AF: 66.7 ± 46.4 mg/dL), as were 4-h TG levels (SL: 144.6 ± 84.2 mg/dL; AL: 171.4 ± 88.2 mg/dL; AF: 157.7 ± 76.7 mg/dL; p = 0.49). Similarly, total and incremental area under the curve (AUC) were not significantly different across the trials (p = 0.12 and 0.91, respectively). Conclusion: The TG results of the clinically feasible, abbreviated protocol were similar to those of the more exhaustive standard protocol. The AF protocol could be a valid and feasible clinical tool for measurement of postprandial lipemia and assessment of cardiovascular risk, although studies in larger and more diverse cohorts are needed.