This page has only limited features, please log in for full access.
In the last three decades, we assisted in a shift in the dairy product preferences in developed countries, with an increase in goat-derived products consumption. As already seen in the bovine sector, the diffusion of goat milk consumption has led to an abandonment of the local breeds in favor of the more productive cosmopolitan breeds, with an intense reduction in the livestock biodiversity. However, in several studies, it has been shown that local breeds present unique features such as a robust behavior towards the local climate and a high quality of their dairy products. This study aims to assess the differences between the basic lactation variables (milk yield, fat %, protein %, lactose % and somatic cell count) in the Alpine goat and the local breed Verzasca goat from a Bayesian point of observation. Results indicate that the Nera di Verzasca goat has overall lactation characteristics similar toa cosmopolite breed such as the Alpine goat, except for the milk daily yield reduced with respect to Alpine goats (1.68 ± 0.84 and 2.14 ± 1.03, respectively). Moreover, the Verzasca goat showed a tendency to maintain a lower level of somatic cell count along with lactation (798 ± 1734 and 1216 ± 2828, respectively). These findings help to increase the knowledge about the Italian local goat breeds and are important in the optic of biodiversity conservation.
Stella Agradi; Alessia Gazzonis; Giulio Curone; Massimo Faustini; Susanna Draghi; Gabriele Brecchia; Daniele Vigo; Maria Manfredi; Sergio Zanzani; Luisa Pulinas; Majlind Sulce; Albana Munga; Marta Castrica; Laura Menchetti. Lactation Characteristics in Alpine and Nera di Verzasca Goats in Northern Italy: A Statistical Bayesian Approach. Applied Sciences 2021, 11, 7235 .
AMA StyleStella Agradi, Alessia Gazzonis, Giulio Curone, Massimo Faustini, Susanna Draghi, Gabriele Brecchia, Daniele Vigo, Maria Manfredi, Sergio Zanzani, Luisa Pulinas, Majlind Sulce, Albana Munga, Marta Castrica, Laura Menchetti. Lactation Characteristics in Alpine and Nera di Verzasca Goats in Northern Italy: A Statistical Bayesian Approach. Applied Sciences. 2021; 11 (16):7235.
Chicago/Turabian StyleStella Agradi; Alessia Gazzonis; Giulio Curone; Massimo Faustini; Susanna Draghi; Gabriele Brecchia; Daniele Vigo; Maria Manfredi; Sergio Zanzani; Luisa Pulinas; Majlind Sulce; Albana Munga; Marta Castrica; Laura Menchetti. 2021. "Lactation Characteristics in Alpine and Nera di Verzasca Goats in Northern Italy: A Statistical Bayesian Approach." Applied Sciences 11, no. 16: 7235.
High-pressure (HP) treatment could lead to several advantages when applied to fish and seafood since it would affect the extension of the shelf life of this highly perishable food. In this regard, this study aimed to evaluate the effect of high-pressure treatment (500 MPa for 2 min at a temperature of 4 °C) on changes in quality on two different kinds of fresh fish fillets (Salmo salar and Pleuronectes platessa). Specifically, physico-chemical (VOCs, untargeted metabolomics spectra, pH and color), microbiological (Enterobacteriaceae, Pseudomonas spp., mesophilic and psychrotrophic bacteria) and sensory traits were evaluated at different days of refrigerated storage. From the results obtained, it is possible to state that the high pressure significantly (p ≤ 0.05) reduced microbial growth for each investigated microorganism. Regarding the colorimetric coordinates, no remarkable effects on a* and b* indices were found, while a significant effect (p = 0.01) was observed on the colorimetric index L*, making the HP-treated samples lighter than their respective controls. The sensory analysis showed that for the odor attribute, the HP treatment seems to have had a stabilizing action during shelf-life. Moreover, the treated samples obtained a better score than the respective controls (p ≤ 0.05). With regards to texture and appearance attributes, the treatment seems to have had a significant (p ≤ 0.05) effect, making the treated samples more compact and opaque than controls, therefore resulting in the loss of the characteristics of raw fish for the treated samples. Moreover, from a chemical point of view, HP treatment prevents the development of volatile sulfides and delays the formation of histamine (p ≤ 0.05). Very interestingly, the metabolomic approach revealed novel dipeptide markers for the HP procedure.
Marta Castrica; Radmila Pavlovic; Claudia Balzaretti; Giulio Curone; Gabriele Brecchia; Emma Copelotti; Sara Panseri; Davide Pessina; Claudio Arnoldi; Luca Chiesa. Effect of High-Pressure Processing on Physico-Chemical, Microbiological and Sensory Traits in Fresh Fish Fillets (Salmo salar and Pleuronectes platessa). Foods 2021, 10, 1775 .
AMA StyleMarta Castrica, Radmila Pavlovic, Claudia Balzaretti, Giulio Curone, Gabriele Brecchia, Emma Copelotti, Sara Panseri, Davide Pessina, Claudio Arnoldi, Luca Chiesa. Effect of High-Pressure Processing on Physico-Chemical, Microbiological and Sensory Traits in Fresh Fish Fillets (Salmo salar and Pleuronectes platessa). Foods. 2021; 10 (8):1775.
Chicago/Turabian StyleMarta Castrica; Radmila Pavlovic; Claudia Balzaretti; Giulio Curone; Gabriele Brecchia; Emma Copelotti; Sara Panseri; Davide Pessina; Claudio Arnoldi; Luca Chiesa. 2021. "Effect of High-Pressure Processing on Physico-Chemical, Microbiological and Sensory Traits in Fresh Fish Fillets (Salmo salar and Pleuronectes platessa)." Foods 10, no. 8: 1775.
Goji berry shows a wide range of beneficial properties in human health, but only a few studies evaluated its effects in livestock animals. The objective of this research was to assess the effects of goji berry supplementation on the hormonal profile, productive, and reproductive performance of does. Two months before artificial insemination, 105 nulliparous does were randomly divided into three groups (n = 35) based on the dietary treatment: commercial diet (C), or a diet supplemented with either 1% (G1), or 3% (G3) of goji berry, respectively. The results showed that receptivity was higher in G1 than in the C group (p< 0.05). Trends toward significance for differences between the G1 and G3 groups in marginal means of LH concentrations (p = 0.059), and between G1 and C in LH AUC values (p = 0.078), were evidenced. Estrogen concentrations showed a more fluctuating trend but a significant interaction effect (p< 0.001). The G1 group showed higher litter weight than C at birth (p = 0.008) and weaning (p< 0.001), as well as higher litter size at weaning (p = 0.020). The G1 group also exhibited the highest mean milk production (p< 0.01). In conclusion, goji berry influenced reproductive and productive performance, probably via modulating hormonal patterns and milk production in rabbits. However, further studies are needed to validate these preliminary results.
Egon Andoni; Giulio Curone; Stella Agradi; Olimpia Barbato; Laura Menchetti; Daniele Vigo; Riccardo Zelli; Elisa Cotozzolo; Maria Ceccarini; Massimo Faustini; Alda Quattrone; Marta Castrica; Gabriele Brecchia. Effect of Goji Berry (Lycium barbarum) Supplementation on Reproductive Performance of Rabbit Does. Animals 2021, 11, 1672 .
AMA StyleEgon Andoni, Giulio Curone, Stella Agradi, Olimpia Barbato, Laura Menchetti, Daniele Vigo, Riccardo Zelli, Elisa Cotozzolo, Maria Ceccarini, Massimo Faustini, Alda Quattrone, Marta Castrica, Gabriele Brecchia. Effect of Goji Berry (Lycium barbarum) Supplementation on Reproductive Performance of Rabbit Does. Animals. 2021; 11 (6):1672.
Chicago/Turabian StyleEgon Andoni; Giulio Curone; Stella Agradi; Olimpia Barbato; Laura Menchetti; Daniele Vigo; Riccardo Zelli; Elisa Cotozzolo; Maria Ceccarini; Massimo Faustini; Alda Quattrone; Marta Castrica; Gabriele Brecchia. 2021. "Effect of Goji Berry (Lycium barbarum) Supplementation on Reproductive Performance of Rabbit Does." Animals 11, no. 6: 1672.
This study aims to find reliable strategies for advancing first mating and shortening the period from weaning to first lambing in ewe lambs. Sarda ewe lambs (n = 400) were selected from two farms and allocated into four separated groups of 100, all of which were exposed to fertile, adult rams over the course of a 50-day breeding period. The first treatment group (RMR) was exposed to four melatonin-treated rams which were replaced every ten days, whilst the second treatment group (RM) was exposed to four melatonin-treated rams which were not replaced. Alternatively, the first control group (RCR) was exposed to four untreated rams which were replaced every ten days, whilst the second control group (RC) was exposed to four untreated rams which were not replaced. In each group, lambing dates, fertility rate, litter size, and distance in days from ram introduction to lambing (DRIL) were recorded. The highest fertility rate was recorded in the RMR group (p ≤ 0.05). Shorter DRIL (p ≤ 0.01) and higher lambing concentrations were recorded in the RM and RMR groups as compared to the controls. The findings indicate that melatonin treatment of rams and their replacement at 10-day intervals results in earlier onset of first mating, increased fertility rate in ewe lambs, and a higher number of ewes that lambs in a shorter time frame.
Giovanni Cosso; Sebastiano Luridiana; Luisa Pulinas; Giulio Curone; Giulia Pich; Vincenzo Carcangiu; Maria Mura. Melatonin Treatment in Rams and Their Replacement with Novel Treated Rams Advance First Lambing and Increase Fertility in Sarda Ewe Lambs. Animals 2021, 11, 1227 .
AMA StyleGiovanni Cosso, Sebastiano Luridiana, Luisa Pulinas, Giulio Curone, Giulia Pich, Vincenzo Carcangiu, Maria Mura. Melatonin Treatment in Rams and Their Replacement with Novel Treated Rams Advance First Lambing and Increase Fertility in Sarda Ewe Lambs. Animals. 2021; 11 (5):1227.
Chicago/Turabian StyleGiovanni Cosso; Sebastiano Luridiana; Luisa Pulinas; Giulio Curone; Giulia Pich; Vincenzo Carcangiu; Maria Mura. 2021. "Melatonin Treatment in Rams and Their Replacement with Novel Treated Rams Advance First Lambing and Increase Fertility in Sarda Ewe Lambs." Animals 11, no. 5: 1227.
The economy in Mediterranean areas is tightly linked to the evolution of the sheep-farming system; therefore, improvement in ewe’s reproductive performance is essential in the developing countries of this area. MTNR1A is the gene coding for Melatonin receptor 1 (MT1), and it is considered to be involved in the reproductive activity in sheep. The aims of this study were: (1) identifying the polymorphisms from the entire MTNR1A coding region and promoter in Lebanese Awassi sheep flocks, and (2) investigating the association between the found polymorphisms and the reproductive performance, assessed as lambing rate, litter size, and days to lambing (DTL). The study was conducted in two districts of Lebanon, where 165 lactating ewes, aged 5.2 ± 1.5 years, with body condition score (BCS) 3.3 ± 0.4, were chosen and exposed to adult and fertile rams. From 150 to 220 days after ram introduction, lambing dates and litter sizes were registered. This study provided the entire coding region of the MTNR1A receptor gene in the Awassi sheep breed. Thirty-one single nucleotide polymorphisms (SNPs) were detected, five of which were missense mutations. The H2, H3, and H4 haplotypes were associated with lower DTL (p < 0.05), as well as the SNPs rs430181568 and rs40738822721, named from now on SNP20 and SNP21, respectively. These SNPs were totally linked and can be considered as a single marker. The ewes carrying the C allele at both these polymorphic sites advanced their reproductive recovery (p < 0.05). These results are essential for improving reproductive management and obtaining advanced lambing in Awassi ewes.
Giovanni Cosso; Michella Nehme; Sebastiano Luridiana; Luisa Pulinas; Giulio Curone; Chadi Hosri; Vincenzo Carcangiu; Maria Mura. Detection of Polymorphisms in the MTNR1A Gene and Their Association with Reproductive Performance in Awassi Ewes. Animals 2021, 11, 583 .
AMA StyleGiovanni Cosso, Michella Nehme, Sebastiano Luridiana, Luisa Pulinas, Giulio Curone, Chadi Hosri, Vincenzo Carcangiu, Maria Mura. Detection of Polymorphisms in the MTNR1A Gene and Their Association with Reproductive Performance in Awassi Ewes. Animals. 2021; 11 (2):583.
Chicago/Turabian StyleGiovanni Cosso; Michella Nehme; Sebastiano Luridiana; Luisa Pulinas; Giulio Curone; Chadi Hosri; Vincenzo Carcangiu; Maria Mura. 2021. "Detection of Polymorphisms in the MTNR1A Gene and Their Association with Reproductive Performance in Awassi Ewes." Animals 11, no. 2: 583.
In ruminants, the role of melatonin in the control of reproductive seasonality is well reported, but it is still little known about its action on milk traits and on the immune system. The aim of this study was to assess the effect of melatonin on milk yield and composition, somatic cell count (SCC), some cytokine blood concentration and reproductive resumption in sheep. One hundred lactating sheep were allocated to two groups (of 50 sheep each), M (treated with melatonin) and C (controls), and exposed to the rams for 50 d. Time period in days from ram introduction to lambing (TRIL) and litter size were recorded. Every 15 d, from 1 March to 30 April, the individual daily milk yield was registered and milk composition and SCC, were analysed. The levels of Interleukin 2 and 6 (IL-2 and 6) and tumour necrosis factor-alpha (Tnf-α) were evaluated every 15 d. The highest fertility rate (p < .01) and the shortest TRIL (p < .05) was recorded in M group. Milk yield and composition were similar between groups. The somatic cells decreased continuously in the treated ewes (p < .01) and increased continuously in untreated ewes (p < .05). IL-2 and IL-6 increased in M group, although no statistical differences were found between groups. Melatonin administration improved reproductive efficiency, did not affect milk yield and composition and decreased somatic cells. This last effect could help to maintain healthy mammary gland and encourage farmers to reduce the use of drugs against mastitis.
Giovanni Cosso; Maria Consuelo Mura; Luisa Pulinas; Giulio Curone; Daniele Vigo; Vincenzo Carcangiu; Sebastiano Luridiana. Effects of melatonin treatment on milk traits, reproductive performance and immune response in Sarda dairy sheep. Italian Journal of Animal Science 2021, 20, 632 -639.
AMA StyleGiovanni Cosso, Maria Consuelo Mura, Luisa Pulinas, Giulio Curone, Daniele Vigo, Vincenzo Carcangiu, Sebastiano Luridiana. Effects of melatonin treatment on milk traits, reproductive performance and immune response in Sarda dairy sheep. Italian Journal of Animal Science. 2021; 20 (1):632-639.
Chicago/Turabian StyleGiovanni Cosso; Maria Consuelo Mura; Luisa Pulinas; Giulio Curone; Daniele Vigo; Vincenzo Carcangiu; Sebastiano Luridiana. 2021. "Effects of melatonin treatment on milk traits, reproductive performance and immune response in Sarda dairy sheep." Italian Journal of Animal Science 20, no. 1: 632-639.
The microbiota is extremely important for the animal’s health, but, to date, knowledge on the intestinal microbiota of the rabbit is very limited. This study aimed to describe bacterial populations that inhabit the different gastrointestinal compartments of the rabbit: stomach, duodenum, jejunum, ileum, caecum, and colon. Samples of the luminal content from all compartments of 14 healthy New White Zealand rabbits were collected at slaughter and analyzed using next generation 16S rRNA Gene Sequencing. The findings uncovered considerable differences in the taxonomic levels among the regions of the digestive tract. Firmicutes were the most abundant phylum in all of the sections (45.9%), followed by Bacteroidetes in the large intestine (38.9%) and Euryarchaeota in the foregut (25.9%). Four clusters of bacterial populations were observed along the digestive system: (i) stomach, (ii) duodenum and jejunum, (iii) ileum, and (iv) large intestine. Caecum and colon showed the highest richness and diversity in bacterial species, while the highest variability was found in the upper digestive tract. Knowledge of the physiological microbiota of healthy rabbits could be important for preserving the health and welfare of the host as well as for finding strategies to manipulate the gut microbiota in order to also promote productive performance.
Elisa Cotozzolo; Paola Cremonesi; Giulio Curone; Laura Menchetti; Federica Riva; Filippo Biscarini; Maria Laura Marongiu; Marta Castrica; Bianca Castiglioni; Dino Miraglia; Sebastiano Luridiana; Gabriele Brecchia. Characterization of Bacterial Microbiota Composition along the Gastrointestinal Tract in Rabbits. Animals 2020, 11, 31 .
AMA StyleElisa Cotozzolo, Paola Cremonesi, Giulio Curone, Laura Menchetti, Federica Riva, Filippo Biscarini, Maria Laura Marongiu, Marta Castrica, Bianca Castiglioni, Dino Miraglia, Sebastiano Luridiana, Gabriele Brecchia. Characterization of Bacterial Microbiota Composition along the Gastrointestinal Tract in Rabbits. Animals. 2020; 11 (1):31.
Chicago/Turabian StyleElisa Cotozzolo; Paola Cremonesi; Giulio Curone; Laura Menchetti; Federica Riva; Filippo Biscarini; Maria Laura Marongiu; Marta Castrica; Bianca Castiglioni; Dino Miraglia; Sebastiano Luridiana; Gabriele Brecchia. 2020. "Characterization of Bacterial Microbiota Composition along the Gastrointestinal Tract in Rabbits." Animals 11, no. 1: 31.
This study examined the effects of goji berries dietary supplementation on the energetic metabolism of doe. Thirty days before artificial insemination, 75 New Zealand White does were assigned to three different diets: commercial standard diet (C) and supplemented with 1% (LG) and 3% (HG) of goji berries, respectively. Body conditions, hormones and metabolites were monitored until weaning. Body weight and BCS were higher in HG than C (p < 0.05). LG showed lower T3/T4 ratio and cortisol concentrations (p < 0.05) and tended to have lower indices of insulin resistances (p < 0.1) than HG. Compared to control, leptin was higher in HG at AI (p < 0.01) and in LG during lactation (p < 0.05). Two principal components were extracted by multivariate analysis describing the relationships between (1) non-esterified fatty acids, insulin and glucose levels, and (2) body conditions and leptin metabolism. The first component highlighted the energy deficit and the insulin resistance of the does during pregnancy and lactation. The second one showed that leptin, body weight and Body Condition Score (BCS) enhance as levels of goji berries in the diet increase. Thus, the effects of goji supplementation are dose-dependent: an improvement on energy metabolism was achieved with a low-dose while the highest dose could determine excessive fattening and insulin resistance in does.
Laura Menchetti; Giulio Curone; Egon Andoni; Olimpia Barbato; Alessandro Troisi; Bernard Fioretti; Angela Polisca; Michela Codini; Claudio Canali; Daniele Vigo; Gabriele Brecchia. Impact of Goji Berries (Lycium barbarum) Supplementation on the Energy Homeostasis of Rabbit Does: Uni- and Multivariate Approach. Animals 2020, 10, 2000 .
AMA StyleLaura Menchetti, Giulio Curone, Egon Andoni, Olimpia Barbato, Alessandro Troisi, Bernard Fioretti, Angela Polisca, Michela Codini, Claudio Canali, Daniele Vigo, Gabriele Brecchia. Impact of Goji Berries (Lycium barbarum) Supplementation on the Energy Homeostasis of Rabbit Does: Uni- and Multivariate Approach. Animals. 2020; 10 (11):2000.
Chicago/Turabian StyleLaura Menchetti; Giulio Curone; Egon Andoni; Olimpia Barbato; Alessandro Troisi; Bernard Fioretti; Angela Polisca; Michela Codini; Claudio Canali; Daniele Vigo; Gabriele Brecchia. 2020. "Impact of Goji Berries (Lycium barbarum) Supplementation on the Energy Homeostasis of Rabbit Does: Uni- and Multivariate Approach." Animals 10, no. 11: 2000.
Forty-two New Zealand White rabbits (n = 21/group) were fed with two different diets: a commercial diet (control group) and a diet supplemented with goji berries (3% w/w). After slaughtering, the effect of dietary supplementation on microbiological, physico-chemical, and sensory characteristics of the rabbit loins, packed in an oxygen-permeable package, was evaluated at 6 h post mortem (day 0), after 4 and 10 days of refrigerated storage. No relevant results were obtained for pH and total volatile basic Nitrogen (TVBN) values but with regards to the color, some significant differences were observed between the groups. The goji berries (GBs) dietary supplementation had positive effects by reducing thiobarbituric acid reactive substances (TBARS) values in all the observations (p < 0.001). Moreover, microbiological results showed that the supplementation had a significant impact on Lactobacillus spp. (p < 0.001) prevalence, indeed the goji group had higher means on day 0 (p < 0.05) and on day 4 (p < 0.001) than the control group. Lastly, with regards to the consumer’s test, the tasters assigned a higher score to GBs rabbit meatballs and the purchase interest increased when the rabbit diet was known. Overall, these results indicate that the goji berries inclusion in the rabbit diet could represent a valuable strategy to improve quality and sensory traits of meat.
Marta Castrica; Laura Menchetti; Claudia M. Balzaretti; Raffaella Branciari; David Ranucci; Elisa Cotozzolo; Daniele Vigo; Giulio Curone; Gabriele Brecchia; Dino Miraglia. Impact of Dietary Supplementation with Goji Berries (Lycium barbarum) on Microbiological Quality, Physico-Chemical, and Sensory Characteristics of Rabbit Meat. Foods 2020, 9, 1480 .
AMA StyleMarta Castrica, Laura Menchetti, Claudia M. Balzaretti, Raffaella Branciari, David Ranucci, Elisa Cotozzolo, Daniele Vigo, Giulio Curone, Gabriele Brecchia, Dino Miraglia. Impact of Dietary Supplementation with Goji Berries (Lycium barbarum) on Microbiological Quality, Physico-Chemical, and Sensory Characteristics of Rabbit Meat. Foods. 2020; 9 (10):1480.
Chicago/Turabian StyleMarta Castrica; Laura Menchetti; Claudia M. Balzaretti; Raffaella Branciari; David Ranucci; Elisa Cotozzolo; Daniele Vigo; Giulio Curone; Gabriele Brecchia; Dino Miraglia. 2020. "Impact of Dietary Supplementation with Goji Berries (Lycium barbarum) on Microbiological Quality, Physico-Chemical, and Sensory Characteristics of Rabbit Meat." Foods 9, no. 10: 1480.
The aim of this study was to characterize the MTNR1A locus in Sarda sheep breed, in order to identify potential single nucleotide polymorphisms (SNPs) associated with reproductive resumption. The reproductive performance of 200 lactating ewes, aged 3–5 years, with body condition score (BCS) 2.5–4.0, at least at their third lambing were monitored for two consecutive years. In both year the enrolled ewes were exposed for 100 days to 10 adult, fertile rams. Mating, pregnancy and lambing for each ewe were recorded in order to evaluate differences in reproductive performance according to the analysed genotypes. From individual blood samples, DNA was extracted to amplify and to sequence promotor, the coding region, a part of intron and of 3′ Untranslated region (3′ UTR) of the MTNR1A gene. A total number of 29 SNPs were found (named SNP1 to SNP29), five of which caused also amino acid changes. The polymorphic sites found at positions g.17355452C > T (SNP16, rs430181568) and g.17355358C > T (SNP17, rs407388227) were linked (D’ = 1 and r2 = 1) and showed a significant association to DRIL trait (distance in days from ram introduction to lambing). In both years, the ewes carrying C/C genotype in both these polymorphic sites showed the lowest DRIL compared to the other genotypes (P < 0.05). The ewes carrying C/C and T/C genotype exhibited the lambing peak at 170 days, and approximately 60% of the total lambing at 180 days from the ram introduction. Instead, ewes carrying T/T genotype showed the lambing peak around 200 days after ram introduction. Six haplotypes have been identified and the most frequent haplotype was also associated with lower DRIL (P < 0.05). Litter size displayed no statistical significance either among genotypes or among haplotypes. This study provided the major part of the MTNR1A gene in Sarda sheep breed and evidenced that SNP17 is associated with a shorter DRIL. The obtained results underlined the role of this polymorphism in improving reproductive efficiency in Sarda sheep and provides a suitable information for improving genetic selection.
Sebastiano Luridiana; Giovanni Cosso; Luisa Pulinas; Maria Veronica Di Stefano; Giulio Curone; Vincenzo Carcangiu; Maria Consuelo Mura. New polymorphisms at MTNR1A gene and their association with reproductive resumption in sarda breed sheep. Theriogenology 2020, 158, 438 -444.
AMA StyleSebastiano Luridiana, Giovanni Cosso, Luisa Pulinas, Maria Veronica Di Stefano, Giulio Curone, Vincenzo Carcangiu, Maria Consuelo Mura. New polymorphisms at MTNR1A gene and their association with reproductive resumption in sarda breed sheep. Theriogenology. 2020; 158 ():438-444.
Chicago/Turabian StyleSebastiano Luridiana; Giovanni Cosso; Luisa Pulinas; Maria Veronica Di Stefano; Giulio Curone; Vincenzo Carcangiu; Maria Consuelo Mura. 2020. "New polymorphisms at MTNR1A gene and their association with reproductive resumption in sarda breed sheep." Theriogenology 158, no. : 438-444.
S. Mann; G. Curone; T.L. Chandler; P. Moroni; J. Cha; Ruchika Bhawal; S. Zhang. Heat treatment of bovine colostrum: I. Effects on bacterial and somatic cell counts, immunoglobulin, insulin, and IGF-I concentrations, as well as the colostrum proteome. Journal of Dairy Science 2020, 103, 9368 -9383.
AMA StyleS. Mann, G. Curone, T.L. Chandler, P. Moroni, J. Cha, Ruchika Bhawal, S. Zhang. Heat treatment of bovine colostrum: I. Effects on bacterial and somatic cell counts, immunoglobulin, insulin, and IGF-I concentrations, as well as the colostrum proteome. Journal of Dairy Science. 2020; 103 (10):9368-9383.
Chicago/Turabian StyleS. Mann; G. Curone; T.L. Chandler; P. Moroni; J. Cha; Ruchika Bhawal; S. Zhang. 2020. "Heat treatment of bovine colostrum: I. Effects on bacterial and somatic cell counts, immunoglobulin, insulin, and IGF-I concentrations, as well as the colostrum proteome." Journal of Dairy Science 103, no. 10: 9368-9383.
S. Mann; G. Curone; T.L. Chandler; A. Sipka; J. Cha; Ruchika Bhawal; S. Zhang. Heat treatment of bovine colostrum: II. Effects on calf serum immunoglobulin, insulin, and IGF-I concentrations, and the serum proteome. Journal of Dairy Science 2020, 103, 9384 -9406.
AMA StyleS. Mann, G. Curone, T.L. Chandler, A. Sipka, J. Cha, Ruchika Bhawal, S. Zhang. Heat treatment of bovine colostrum: II. Effects on calf serum immunoglobulin, insulin, and IGF-I concentrations, and the serum proteome. Journal of Dairy Science. 2020; 103 (10):9384-9406.
Chicago/Turabian StyleS. Mann; G. Curone; T.L. Chandler; A. Sipka; J. Cha; Ruchika Bhawal; S. Zhang. 2020. "Heat treatment of bovine colostrum: II. Effects on calf serum immunoglobulin, insulin, and IGF-I concentrations, and the serum proteome." Journal of Dairy Science 103, no. 10: 9384-9406.
Animal health is affected by many factors such as metabolic stress, the immune system, and epidemiological features that interconnect. The immune system has evolved along with the phylogenetic evolution as a highly refined sensing and response system, poised to react against diverse infectious and non-infectious stressors for better survival and adaptation. It is now known that high genetic merit for milk yield is correlated with a defective control of the inflammatory response, underlying the occurrence of several production diseases. This is evident in the mastitis model where high-yielding dairy cows show high disease prevalence of the mammary gland with reduced effectiveness of the innate immune system and poor control over the inflammatory response to microbial agents. There is growing evidence of epigenetic effects on innate immunity genes underlying the response to common microbial agents. The aforementioned agents, along with other non-infectious stressors, can give rise to abnormal activation of the innate immune system, underlying serious disease conditions, and affecting milk yield. Furthermore, the microbiome also plays a role in shaping immune functions and disease resistance as a whole. Accordingly, proper modulation of the microbiome can be pivotal to successful disease control strategies. These strategies can benefit from a fundamental re-appraisal of native cattle breeds as models of disease resistance based on successful coping of both infectious and non-infectious stressors.
Valerio Bronzo; Vincenzo Lopreiato; Federica Riva; Massimo Amadori; Giulio Curone; Maria Filippa Addis; Paola Cremonesi; Paolo Moroni; Erminio Trevisi; Bianca Castiglioni. The Role of Innate Immune Response and Microbiome in Resilience of Dairy Cattle to Disease: The Mastitis Model. Animals 2020, 10, 1397 .
AMA StyleValerio Bronzo, Vincenzo Lopreiato, Federica Riva, Massimo Amadori, Giulio Curone, Maria Filippa Addis, Paola Cremonesi, Paolo Moroni, Erminio Trevisi, Bianca Castiglioni. The Role of Innate Immune Response and Microbiome in Resilience of Dairy Cattle to Disease: The Mastitis Model. Animals. 2020; 10 (8):1397.
Chicago/Turabian StyleValerio Bronzo; Vincenzo Lopreiato; Federica Riva; Massimo Amadori; Giulio Curone; Maria Filippa Addis; Paola Cremonesi; Paolo Moroni; Erminio Trevisi; Bianca Castiglioni. 2020. "The Role of Innate Immune Response and Microbiome in Resilience of Dairy Cattle to Disease: The Mastitis Model." Animals 10, no. 8: 1397.
This study aimed to evaluate the relationships between fatty acids and the pattern that most contributes to discriminate between two farming systems, in which the main difference was the practice, or not, of alpine summer-grazing. Milk and cheese were sampled every month in two farms of Original Brown cows identical under geographical location and management during no grazing season point of view in the 2018 season. Fatty acids concentrations were determined by gas chromatography. The principal component analysis extracted three components (PCs). Mammary gland de novo synthetized fatty acids (C14:0, C14:1 n9, and C16:0) and saturated and monosaturated C18 fatty acids (C18:0, C18:1 n9c) were inversely associated in the PC1; PC2 included polyunsaturated C18 fatty acids (C18:2 n6c, C18:3 n3) and C15:0 while conjugated linoleic acid (CLA n9c, n11t) and fatty acids containing 20 or more carbon atoms (C21:0, C20:5 n3) were associated in the PC3. The processes of rumen fermentation and de novo synthesis in mammary gland that are, in turn, influenced by diet, could explain the relationships between fatty acids within each PC. The discriminant analyses showed that the PC2 included the fatty acids profile that best discriminated between the two farming systems, followed by PC3 and, lastly, PC1. This model, if validated, could be an important tool to the dairy industry.
Stella Agradi; Giulio Curone; Daniele Negroni; Daniele Vigo; Gabriele Brecchia; Valerio Bronzo; Sara Panseri; Luca Maria Chiesa; Tanja Peric; Doina Danes; Laura Menchetti. Determination of Fatty Acids Profile in Original Brown Cows Dairy Products and Relationship with Alpine Pasture Farming System. Animals 2020, 10, 1231 .
AMA StyleStella Agradi, Giulio Curone, Daniele Negroni, Daniele Vigo, Gabriele Brecchia, Valerio Bronzo, Sara Panseri, Luca Maria Chiesa, Tanja Peric, Doina Danes, Laura Menchetti. Determination of Fatty Acids Profile in Original Brown Cows Dairy Products and Relationship with Alpine Pasture Farming System. Animals. 2020; 10 (7):1231.
Chicago/Turabian StyleStella Agradi; Giulio Curone; Daniele Negroni; Daniele Vigo; Gabriele Brecchia; Valerio Bronzo; Sara Panseri; Luca Maria Chiesa; Tanja Peric; Doina Danes; Laura Menchetti. 2020. "Determination of Fatty Acids Profile in Original Brown Cows Dairy Products and Relationship with Alpine Pasture Farming System." Animals 10, no. 7: 1231.
This study was conducted to evaluate the changing concentrations of metabolic hormones and metabolites in pregnant (P) and pseudopregnant (PP) rabbit does. Twenty-five New Zealand White rabbit does were submitted to artificial insemination (AI) and then classified as P (n = 15) or PP (n = 10). Blood samples were collected weekly until day 32 post AI. During pregnancy, leptin concentrations were greater on Days 14 and 21 (P < 0.05), while insulin was greater on days 21 and 32 post AI (P < 0.05) compared to PP does. The triiodothyronine/thyroxine (T3/T4) ratio was greater in the first and last week (P < 0.001); whereas, cortisol concentrations were greater in the last week of pregnancy and after parturition (P < 0.01) compared with that of PP does. Non-esterified fatty acids (NEFA) concentrations increased from day 7 until day 32 post AI (P < 0.05). Glucose concentrations were unchanged throughout pregnancy although concentrations were positively associated with litter size. These results indicate concentrations of hormones and metabolites change during pregnancy to ensure energy requirements are met for both the foetuses and the maternal tissues. Physiological hyperleptinemia, hyperinsulinemia, and changes in cortisol as well as thyroid hormones indicate there is an adaptation of metabolic functions induced by pregnancy. These adaptations could be mediated by gonadal steroids because changes mainly occur in the second half of pregnancy when the profile of the sex hormones differs between P and PP does.
Laura Menchetti; Egon Andoni; Olimpia Barbato; Claudio Canali; Alda Quattrone; Daniele Vigo; Michela Codini; Giulio Curone; Gabriele Brecchia. Energy homeostasis in rabbit does during pregnancy and pseudopregnancy. Animal Reproduction Science 2020, 218, 106505 .
AMA StyleLaura Menchetti, Egon Andoni, Olimpia Barbato, Claudio Canali, Alda Quattrone, Daniele Vigo, Michela Codini, Giulio Curone, Gabriele Brecchia. Energy homeostasis in rabbit does during pregnancy and pseudopregnancy. Animal Reproduction Science. 2020; 218 ():106505.
Chicago/Turabian StyleLaura Menchetti; Egon Andoni; Olimpia Barbato; Claudio Canali; Alda Quattrone; Daniele Vigo; Michela Codini; Giulio Curone; Gabriele Brecchia. 2020. "Energy homeostasis in rabbit does during pregnancy and pseudopregnancy." Animal Reproduction Science 218, no. : 106505.
This study investigated the effects of a short-term administration of bovine colostrum (BC) in a TNBS model of induced colitis. Colitis was induced by TNBS treatment after seven days of BC (BC group, n = 12) or saline (control group, n = 12) administration in mice. Clinical signs, histopathological characteristics, expression levels of Toll-like receptor 4 (TLR4), pro- and anti-inflammatory cytokines, and microbial composition were assessed. BC was well tolerated and did not induce any histological damage or clinical symptoms. After TNBS treatment, the BC group showed a reduction in body weight (BW) loss compared to Control (p < 0.05). Moreover, expression levels of TLR4 (p < 0.01), Interleukin-1β (IL-1β; p < 0.001), Interleukin-8 (IL-8; p < 0.001), and Interleukin-10 (IL-10; p < 0.001) were lower in mice administered with BC. Finally, Escherichia coli were higher (p < 0.05), while Enterococci (p < 0.001), Lactobacillus spp. (p < 0.001), and Bifidobacterium spp. (p < 0.05) were lower in Control than BC group. This study confirms that pre-treatment with BC modulates the expression of genes and the count of microbes involved in the etiopathogenesis of colitis.
Laura Menchetti; Giulio Curone; Iulia Elena Filipescu; Olimpia Barbato; Leonardo Leonardi; Gabriella Guelfi; Giovanna Traina; Patrizia Casagrande-Proietti; Federica Riva; Anna Beatrice Casano; Federica Piro; Daniele Vigo; Alda Quattrone; Gabriele Brecchia. The Prophylactic Use of Bovine Colostrum in a Murine Model of TNBS-Induced Colitis. Animals 2020, 10, 492 .
AMA StyleLaura Menchetti, Giulio Curone, Iulia Elena Filipescu, Olimpia Barbato, Leonardo Leonardi, Gabriella Guelfi, Giovanna Traina, Patrizia Casagrande-Proietti, Federica Riva, Anna Beatrice Casano, Federica Piro, Daniele Vigo, Alda Quattrone, Gabriele Brecchia. The Prophylactic Use of Bovine Colostrum in a Murine Model of TNBS-Induced Colitis. Animals. 2020; 10 (3):492.
Chicago/Turabian StyleLaura Menchetti; Giulio Curone; Iulia Elena Filipescu; Olimpia Barbato; Leonardo Leonardi; Gabriella Guelfi; Giovanna Traina; Patrizia Casagrande-Proietti; Federica Riva; Anna Beatrice Casano; Federica Piro; Daniele Vigo; Alda Quattrone; Gabriele Brecchia. 2020. "The Prophylactic Use of Bovine Colostrum in a Murine Model of TNBS-Induced Colitis." Animals 10, no. 3: 492.
Climate change involves different dramatic phenomena including desertification and wildfires, severe storms such as hurricanes and blizzards, increased sea levels resulting in flooding coastal cities and rise of atmospheric CO2 concentration. The alteration of the climate in a specific region affects the life of indigenous animals and humans. The climate changes influence living beings both directly and indirectly. The immune system of animals dramatically suffers the climate instability, making animals more susceptible to infectious and not infectious diseases. Different species of livestock animals respond with similar mechanisms to global warming, but some of them are more susceptible depending on their age, metabolism, and genetic conditions. The selection and study of autochthonous species and breeds, more easily adapted to specific environmental conditions could be an interesting strategy to face livestock rearing in the future.
Joel Filipe; Valentina Herrera; Giulio Curone; Daniele Vigo; Federica Riva. Floods, Hurricanes, and Other Catastrophes: A Challenge for the Immune System of Livestock and Other Animals. Frontiers in Veterinary Science 2020, 7, 1 .
AMA StyleJoel Filipe, Valentina Herrera, Giulio Curone, Daniele Vigo, Federica Riva. Floods, Hurricanes, and Other Catastrophes: A Challenge for the Immune System of Livestock and Other Animals. Frontiers in Veterinary Science. 2020; 7 ():1.
Chicago/Turabian StyleJoel Filipe; Valentina Herrera; Giulio Curone; Daniele Vigo; Federica Riva. 2020. "Floods, Hurricanes, and Other Catastrophes: A Challenge for the Immune System of Livestock and Other Animals." Frontiers in Veterinary Science 7, no. : 1.
Infections of the genital tract can perturb the fertility in humans and animals. Pathogen recognition and activation of innate immunity onset through the pattern recognition receptor activation, such as Toll-like receptor 4 (TLR4), leading to the production of proinflammatory cytokines and mediators. TLR4 is expressed both on leukocytes and nonimmune cells. Rabbit TLR4 shows great similarity to its human counterpart. Moreover, the TLR4 signalling pathway could be modulated by long-chain polyunsaturated fatty acids (LC-PUFA). The objectives of this study were (i) to determine the expression levels of TLR4 and proinflammatory cytokines in the reproductive hypothalamic-gonadal axis of the male rabbit and (ii) to evaluate if the n-3 PUFA-enriched diets can modify their expression levels in the tissues and LC-PUFA profiles in seminal plasma. Fifteen rabbit bucks ( n = 5 /experimental group) were fed with different diets: commercial standard (group C), rich in extruded linseed (10%, group L), and in fish oil (3%, group FO) for 110 days. TLR4, TNF-α, and IL-1βmRNA were ubiquitously expressed throughout the hypothalamic-gonadal axis. However, TLR4 mRNA expression was lower in the hypothalamus than the epididymis ( P < 0.01 ), seminal vesicles ( P < 0.01 ), and pituitary gland ( P < 0.05 ). Dietary enrichment in PUFA did not modify the gene expression profile nor the histological characteristics of the tissues. Conversely in seminal plasma, rabbits fed with L and FO had lower n-6 ( P < 0.05 ), LC-PUFA n-6 ( P < 0.05 ), and n-6/n-3 ratio ( P < 0.05 ) but higher n-3 ( P < 0.001 ) and LC-PUFA n-3 ( P < 0.01 ) compared to the control group. Our study builds a map of the gene expression of TRL4 and proinflammatory cytokines in the reproductive hypothalamic-gonadal axis of the male rabbit, fundamental step for understanding the immune defence mechanisms. Diets enriched in LC-PUFA did not affect basal gene expression but modulated sperm fatty acid composition. Finally, rabbit may be an excellent animal model to study the relationship between inflammation and infertility, and the nutritional modulation of immune functions.
Laura Menchetti; Olimpia Barbato; Monica Sforna; Daniele Vigo; Simona Mattioli; Giulio Curone; Marco Tecilla; Federica Riva; Gabriele Brecchia. Effects of Diets Enriched in Linseed and Fish Oil on the Expression Pattern of Toll-Like Receptors 4 and Proinflammatory Cytokines on Gonadal Axis and Reproductive Organs in Rabbit Buck. Oxidative Medicine and Cellular Longevity 2020, 2020, 1 -10.
AMA StyleLaura Menchetti, Olimpia Barbato, Monica Sforna, Daniele Vigo, Simona Mattioli, Giulio Curone, Marco Tecilla, Federica Riva, Gabriele Brecchia. Effects of Diets Enriched in Linseed and Fish Oil on the Expression Pattern of Toll-Like Receptors 4 and Proinflammatory Cytokines on Gonadal Axis and Reproductive Organs in Rabbit Buck. Oxidative Medicine and Cellular Longevity. 2020; 2020 ():1-10.
Chicago/Turabian StyleLaura Menchetti; Olimpia Barbato; Monica Sforna; Daniele Vigo; Simona Mattioli; Giulio Curone; Marco Tecilla; Federica Riva; Gabriele Brecchia. 2020. "Effects of Diets Enriched in Linseed and Fish Oil on the Expression Pattern of Toll-Like Receptors 4 and Proinflammatory Cytokines on Gonadal Axis and Reproductive Organs in Rabbit Buck." Oxidative Medicine and Cellular Longevity 2020, no. : 1-10.
IL-1R8 is a member of Interleukin-1 receptor family acting as a negative regulator of inflammation reliant on ILRs and TLRs activation. IL-1R8 role has never been evaluated in acute bacterial mastitis. We first investigated IL-1R8 sequence conservation among different species and its pattern of expression in a wide panel of organs from healthy goats. Then, modulation of IL-1R8 during natural and experimental mammary infection was evaluated and compared in blood, milk and mammary tissues from healthy and Staphylococcus aureus infected goats. IL-1R8 has a highly conserved sequence among vertebrates. Goat IL-1R8 was ubiquitously expressed in epithelial and lymphoid tissues with highest levels in pancreas. IL-1R8 was down-regulated in epithelial mammary cells following S. aureus infection. Interestingly it was up-regulated in leukocytes infiltrating the infected mammary tissues suggesting that it could represent a target of S. aureus immune evasion.
J. Filipe; V. Bronzo; G. Curone; B. Castiglioni; D. Vigo; B. Smith; Valentina Herrera; P. Roccabianca; P. Moroni; F. Riva. Staphylococcus aureus intra-mammary infection affects the expression pattern of IL-R8 in goat. Comparative Immunology, Microbiology and Infectious Diseases 2019, 66, 101339 .
AMA StyleJ. Filipe, V. Bronzo, G. Curone, B. Castiglioni, D. Vigo, B. Smith, Valentina Herrera, P. Roccabianca, P. Moroni, F. Riva. Staphylococcus aureus intra-mammary infection affects the expression pattern of IL-R8 in goat. Comparative Immunology, Microbiology and Infectious Diseases. 2019; 66 ():101339.
Chicago/Turabian StyleJ. Filipe; V. Bronzo; G. Curone; B. Castiglioni; D. Vigo; B. Smith; Valentina Herrera; P. Roccabianca; P. Moroni; F. Riva. 2019. "Staphylococcus aureus intra-mammary infection affects the expression pattern of IL-R8 in goat." Comparative Immunology, Microbiology and Infectious Diseases 66, no. : 101339.
Serum protein distribution and concentration can be affected by different physiological and pathological conditions. The aim of this study was to evaluate the changes in the concentration of serum protein fractions and haptoglobin in clinically healthy dairy buffaloes during late pregnancy and early lactation. Blood and milk samples were collected from 30 buffaloes at around 7 d before expected calving (blood only) and 7, 30 and 50 d after calving. In serum samples, the total protein, haptoglobin, albumin, α1-, α2-, β1-, β2-, γ-globulins, and albumin/globulin ratio (A/G) values were evaluated. In milk, fat%, protein%, lactose%, somatic cell score (SCS) were assessed, along with milk yield (MY) and daily milk production (DMP). The peripartum period significantly influenced (P < 0.005) total protein, albumin, haptoglobin, α2-, β2- and γ-globulins (P < 0.005). Milk yield, DMP and fat% changed significantly throughout the monitoring period (P < 0.005). Milk yield and DMP were positively correlated with total protein, albumin, β2-globulins and A/G ratio, and negatively correlated with haptoglobin and α2-globulins. These results provide new knowledge about the serum protein electrophoretic pattern in Italian Mediterranean Buffaloes during the last phase of pregnancy and early stages of lactation.
Matteo Gianesella; Enrico Fiore; Francesca Arfuso; Domenico Vecchio; Giulio Curone; Massimo Morgante; Elisa Mazzotta; Tamara Badon; Pasquale Rossi; Silvia Bedin; Alessandro Zumbo; Giuseppe Piccione. Serum haptoglobin and protein electrophoretic fraction modifications in buffaloes (Bubalus bubalis) around calving and during early lactation. Journal of Dairy Research 2019, 86, 291 -295.
AMA StyleMatteo Gianesella, Enrico Fiore, Francesca Arfuso, Domenico Vecchio, Giulio Curone, Massimo Morgante, Elisa Mazzotta, Tamara Badon, Pasquale Rossi, Silvia Bedin, Alessandro Zumbo, Giuseppe Piccione. Serum haptoglobin and protein electrophoretic fraction modifications in buffaloes (Bubalus bubalis) around calving and during early lactation. Journal of Dairy Research. 2019; 86 (3):291-295.
Chicago/Turabian StyleMatteo Gianesella; Enrico Fiore; Francesca Arfuso; Domenico Vecchio; Giulio Curone; Massimo Morgante; Elisa Mazzotta; Tamara Badon; Pasquale Rossi; Silvia Bedin; Alessandro Zumbo; Giuseppe Piccione. 2019. "Serum haptoglobin and protein electrophoretic fraction modifications in buffaloes (Bubalus bubalis) around calving and during early lactation." Journal of Dairy Research 86, no. 3: 291-295.