Objectives : The antioxidant activities of two Indian mangrove plants, Bruguiera cylindrica and Ceriops decandra, were investigated. Materials and Methods : Total phenolics and total flavonoid contents of the mangroves were determined using folin-ciocalteu reagent method and aluminium chloride method, respectively. Antioxidant capacity was assessed by the following methods: 1,1-diphenyl-2-picryl hydroxyl (DPPH.) quenching assay; 2,2'- azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS. ⁺ ) cation decolorization test; scavenging capacity towards hydroxyl ion radicals (.OH); reductive capacity; and antihemolytic activity. Results : The mangroves yielded 233.3 ± 0.062 and 283.31 ± 0.04 mg gallic acid equivalent/g phenolic contents and 11.6 ± 0.12 and 15.1 ± 0.02 mg quercetin equivalent/g flavonoid contents. The methanol extracts of both mangroves exhibited high antiradical activity against DPPH., ABTS. ⁺ , and .OH radicals. The reductive capacity of the extracts increased with increasing concentration of samples. The extracts also inhibited H ₂ O ₂ induced hemolysis in cow blood erythrocytes. The antioxidant activities were found stronger than that of the reference standard, butylated hydroxy toluene (BHT). The antioxidant activity of mangrove plants was correlated with total phenolics and flavonoid contents. Conclusion : Both plants can be considered as good sources of natural antioxidants for medicinal uses. Further studies are necessary to isolate active principles responsible for the overall antioxidant activity of the extracts.

In this study, the antioxidative effectiveness, and polyphenolic content of methanol and aqueous extracts of spices such as Lippia adoensis (Koseret), Nigella sativa (Thikur azmud), Piper capense (Timiz), Thymus schimperi (Tosign) and Trachyspermum ammi (Netchazmud), consumed among people of Ethiopia were investigated. The antioxidant activity was assessed via established in vitro assay models such as 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) radical quenching assay, reducing power assay and reactive nitrogen species (RNS) inhibitory potential. Total phenolics content was measured according to Folin-Ciocalteu's method and total flavonoid content was estimated by using Aluminium chloride colorimetric method. The results showed that the total phenolic content was highest in both methanol (720 ± 0.04 mg GAE/100 g extract DW) and aqueous (580 ± 0.08 mg GAE/100 g DW) extracts of L. adoensis. Among the five tested spices, the methanol and aqueous extracts of L. adoensis exerted the strongest DPPH radical quenching activity with IC50values of 49.17 ± 1.26 and 20.99 ± 8.6 μg/mL, respectively. Both methanol and aqueous extracts of L. adoensis showed notable reducing capacity. The highest RNS scavenging activity was shown by both methanol (IC50 597.21 ± 6.99 μg/mL) and aqueous (IC50 = 551.5 ± 28.9 μg/mL) extracts of L. adoensis. High to moderate positive correlations were observed between total phenolic contents and in vitro antioxidant assays. This indicates that the antioxidant activities of the tested spices are attributed to the phenolic contents. The results of the present work revealed that the tested spices demonstrated high phenolic contents and antioxidant properties. Thus, these spices are worth considering as important sources of natural antioxidant agents.

Traditional medicinal plants used by local Oromo people of Tulo District in west Hararghe, Ethiopia, were investigated before people's medicinal knowledge vanishes. Data on medicinal plants and demography were obtained between November 2019 and October 2020 through semi-structured interviews, group discussion and direct guided onsite observation to elicit information from 376 non-traditional and 20 traditional medicine practitioners. Ethnobotanical indices including informant consensus factor (ICF), preference ranking (PR), fidelity level (FL), relative frequency of citation (RFG) and cultural importance (CI) were employed for the data analysis. Moreover; descriptive statistics, t-test, analysis of variance and linear regression were used to reveal the effects of socio-demographic factors on respondents' traditional medicinal knowledge. Totally 104 plants distributed among 98 genera and 55 families were enumerated for the treatment of 60 illnesses. Seventy-seven of these medicinal plants serve to treat human ailments, whereas 11 and 16 of them were used for livestock and for both, respectively. Asteraceae and Lamiaceae were species rich families. Leaves were the most frequently (41.53%) reported structures for the preparation of remedies. Crushing was the principal approach (34.50%) of remedy preparation. Oral administration was frequently (66.08%) used route of application. The highest ICF was observed for swelling and hemorrhoid (0.90) category. Metabolic and degenerative as well as other ailment categories had the least ICF values. About 66% of medicinal plants had FL value of 100%. In PR, G. abyssinica was ranked first to treat cough. RFC values varied from 0.03 to 0.18 with the highest record for Salvia nilotica (0.18) followed by Lepidium sativum, Rydingia integrifolia and Nigella sativa each with 0.16; Euphorbia abyssinica and Asplenium monanthes each with 0.15. Extensive use of land for agricultural purpose was key threat to medicinal plant of Tulo District. All the tested socio-demographic features except religion significantly (P < 0.05) affected the traditional knowledge on medicinal plants possessed by the study population. The results of this study reveals that the people of Tulo District rely on traditional medicine of plant origin, and their indigenous knowledge is instrumental to exploit the most potential plants for further validation. Therefore, the medicinal plant species wealth of the study site and the associated indigenous knowledge need to be preserved.