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In Huang-Huai-Hai Summer Corn Region of China, brief periods of high summer temperatures have been reported with increasing frequency in recent years. Athetis lepigone is a cosmopolitan insect which causes severe damage on summer corn seedlings. To understand how high summer temperatures may affect the population dynamics of A. lepigone, we exposed different developmental stages (1, 2 and 4-day old eggs; 1, 6, 12 and 18-day old larvae; 1, 3 and 6-day old pupae; and 1 and 2-day old female and male adults) to 41 °C for periods of various length (0.5, 1, 2, 4 and 6 h): The rearing temperature (constant 26 °C) was used as control. After heat treatment, all individuals were transferred to a 26 °C climate chamber for further development. The effects on immediate survival, maturation success to adulthood, and female fecundity were studied. Eggs, young larvae, late pupae and newly emerged adults had relatively higher immediate survival rates than the other experimental groups. Heat treatment at the egg and larval stages had no impact on development to adulthood and on female fecundity, while it significantly reduced the survival rate of larvae but not of eggs. Brief exposure to high temperature during the early pupal stage and as adults depressed female fecundity whereas exposure during the late pupal stage had no effect.
Feifei Liang; Zhenying Wang; Kanglai He; Shuxiong Bai; Tiantao Zhang. Effects of brief exposure to high temperatures on the survival and fecundity of Athetis lepigone (Lepidoptera: Noctuidae). Journal of Thermal Biology 2021, 100, 103066 .
AMA StyleFeifei Liang, Zhenying Wang, Kanglai He, Shuxiong Bai, Tiantao Zhang. Effects of brief exposure to high temperatures on the survival and fecundity of Athetis lepigone (Lepidoptera: Noctuidae). Journal of Thermal Biology. 2021; 100 ():103066.
Chicago/Turabian StyleFeifei Liang; Zhenying Wang; Kanglai He; Shuxiong Bai; Tiantao Zhang. 2021. "Effects of brief exposure to high temperatures on the survival and fecundity of Athetis lepigone (Lepidoptera: Noctuidae)." Journal of Thermal Biology 100, no. : 103066.
Yellow Peach Moth (YPM), Conogethes punctiferalis (Guenée), is one of the most destructive maize pests in the Huang-Huai-Hai summer maize region of China. Transgenic Bacillus thuringiensis (Bt) maize provides an effective means to control this insect pest in field trials. However, the establishment of Bt resistance to target pests is endangering the continued success of Bt crops. To use Bt maize against YPM, the baseline susceptibility of the local populations in the targeted areas needs to be verified. Diet-overlay bioassay results showed that all the fourteen YPM populations in China are highly susceptible to Cry1Ab. The LC50 values ranged from 0.35 to 2.38 ng/cm2 over the two years of the collection, and the difference between the most susceptible and most tolerant populations was sevenfold. The upper limit of the LC99 estimates of six pooled populations produced >99% larval mortality for representative eight populations collected in 2020 and was designated as diagnostic concentrations for monitoring susceptibility in YPM populations in China. Hence, we evaluated the laboratory selection of resistance in YPM to Cry1Ab using the diet-overlay bioassay method. Although the resistant ratio was generally low, YPM potentially could evolve resistance to Cry1Ab. The potential developmentof resistance by target pests points out the necessity to implement resistance management strategies for delaying the establishment of pest resistance to Bt crops.
Su Shwe; Sivaprasath Prabu; Yu Chen; Qincheng Li; Dapeng Jing; Shuxiong Bai; Kanglai He; Zhenying Wang. Baseline Susceptibility and Laboratory Selection of Resistance to Bt Cry1Ab Protein of Chinese Populations of Yellow Peach Moth, Conogethes punctiferalis (Guenée). Toxins 2021, 13, 335 .
AMA StyleSu Shwe, Sivaprasath Prabu, Yu Chen, Qincheng Li, Dapeng Jing, Shuxiong Bai, Kanglai He, Zhenying Wang. Baseline Susceptibility and Laboratory Selection of Resistance to Bt Cry1Ab Protein of Chinese Populations of Yellow Peach Moth, Conogethes punctiferalis (Guenée). Toxins. 2021; 13 (5):335.
Chicago/Turabian StyleSu Shwe; Sivaprasath Prabu; Yu Chen; Qincheng Li; Dapeng Jing; Shuxiong Bai; Kanglai He; Zhenying Wang. 2021. "Baseline Susceptibility and Laboratory Selection of Resistance to Bt Cry1Ab Protein of Chinese Populations of Yellow Peach Moth, Conogethes punctiferalis (Guenée)." Toxins 13, no. 5: 335.
In the Asian corn borer (ACB), Ostrinia furnacalis (Guenée), diapause is governed by a multigenetic constitution that responds to daylength and temperature with seasonality. The ACB displays uni- or multivoltinism, depending on its geographic specificity. Hence, warmer temperatures may result in alternation of voltinism in the ACB, which will help in understanding the ecological consequences of climate warming on insects. In the present study, we investigated the voltinism in two natural populations from Harbin (H) and Gongzhuling (G) as well as a laboratory (L) population (established from the H population in 2017) of the ACB, at ambient and elevated atmospheric CO2 (aCO2 390 μL/L and eCO2 750 μL/L) and temperature (aT and Et = At + 2 °C). From the diapause response, both the uni- and multivoltine ecotypes were coexisting in the H population. The neonate occurrence date of 50% individuals that induced diapause was ca. 10 days later in the G population than in the H population, but it was about 10 days earlier than in the L population. Comparing to the dates of onset and the peak of diapause induction, the G and L populations were less variable than the H population in response to a short and/or shortening daylength in the field. The univoltine individuals could not be eliminated completely after 19 generations of selection. Diapause incidence decreased with a climate-warming scenario, which was temporally specific and could be overridden by significantly low daily average temperatures. The eCO2 did not directly impact the voltinism. On the basis of voltinism, the H population was sympatric for uni- and multivoltine ecotypes, with multivoltinism being dominant. The univoltinism trait was recessive. Climate warming could significantly override the effect of photoperiod, which was yearly dependent. Warmer temperatures and a decreased latitude (shortened daylength), and their interaction, would drive ACB evolution toward diapause homogeneity for multivoltinism.
Kai-Qiang Liu; Lian-Xia Wang; Tian-Tao Zhang; Shu-Xiong Bai; Ke-Qin Wang; Zhen-Ying Wang; Kang-Lai He; William Hutchison. Voltine Ecotypes of the Asian Corn Borer and Their Response to Climate Warming. Insects 2021, 12, 232 .
AMA StyleKai-Qiang Liu, Lian-Xia Wang, Tian-Tao Zhang, Shu-Xiong Bai, Ke-Qin Wang, Zhen-Ying Wang, Kang-Lai He, William Hutchison. Voltine Ecotypes of the Asian Corn Borer and Their Response to Climate Warming. Insects. 2021; 12 (3):232.
Chicago/Turabian StyleKai-Qiang Liu; Lian-Xia Wang; Tian-Tao Zhang; Shu-Xiong Bai; Ke-Qin Wang; Zhen-Ying Wang; Kang-Lai He; William Hutchison. 2021. "Voltine Ecotypes of the Asian Corn Borer and Their Response to Climate Warming." Insects 12, no. 3: 232.
The yellow peach moth, Conogethes punctiferalis (Guenée) has become a major insect pest in Huang-Huai-Hai summer corn region of China in recent years. Larvae directly threatens the yield and quality of corn by boring into corn ear and feeding. Currently, there are few studies to use the parasitoids, Trichogramma, to control the pest C. punctiferalis, so this study firstly evaluated the parasitism, suitability and oviposition behaviour of Trichogramma on C. punctiferalis eggs at different ages in laboratory. We found that C. punctiferalis eggs at different ages could be parasitised by four Trichogramma strains, but the number of parasitised eggs gradually decreased with egg age. Among them, the Xd strain of T. dendrolimi exhibited a better acceptance to old age eggs, which parasitised 12.8 host eggs within 24 h. The percentage of female progeny of Xd strain was the highest among the four strains when young eggs were provided. Parasitoids took more drilling time on old eggs than that on young ones, however, the Xd strain took 37.07s, 36.60s and 44.27s on young, middle and old eggs, respectively, and with no significant difference. The current study has shown that the Xd strain of T. dendrolimi performed better on the parasitism, the acceptance to old age eggs, the host handing time and the suitability of progeny than other strains, indicating that this strain may be a potential biocontrol agent for C. punctiferalis.
Wanbin Chen; Kanglai He; Qinying Wang; Zhenying Wang. Effects of yellow peach moth Conogethes punctiferalis egg age on parasitism and oviposition behaviour of four indigenous Trichogramma strains in China. Biocontrol Science and Technology 2021, 31, 739 -753.
AMA StyleWanbin Chen, Kanglai He, Qinying Wang, Zhenying Wang. Effects of yellow peach moth Conogethes punctiferalis egg age on parasitism and oviposition behaviour of four indigenous Trichogramma strains in China. Biocontrol Science and Technology. 2021; 31 (7):739-753.
Chicago/Turabian StyleWanbin Chen; Kanglai He; Qinying Wang; Zhenying Wang. 2021. "Effects of yellow peach moth Conogethes punctiferalis egg age on parasitism and oviposition behaviour of four indigenous Trichogramma strains in China." Biocontrol Science and Technology 31, no. 7: 739-753.
Yellow peach moth, Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae), is a very destructive fruit borer in Eastern Asia and Australia. A successful artificial diet and rearing protocol are urgently needed for its biological control and research. In this study, we developed and assessed four artificial diets: AD‐1, AD‐2, AD‐3 and AD‐4, for rearing yellow peach moth larvae. AD‐1 is effective supplements, leading to the larval survival rate of 82.05%, percentage of pupation 79.48%, mean pupa weight of 76.53 mg, emergence rate of 95.25%, and an adult fecundity rate of 121 eggs/female. Using it to feed can shorten the whole generation developmental period to 33 days. Based on this diet AD‐1, we also develop a suitable mass rearing method. During the larval stages providing three‐ to four‐time fresh diet can keep a healthy larval colony.
Dapeng Jing; Tiantao Zhang; Shuxiong Bai; Kanglai He; Sivaprasath Prabu; Zhenying Wang. Artificial diet development for mass rearing and its effect on the reproduction of yellow peach moth, Conogethes punctiferalis (Guenée). Entomological Research 2021, 51, 127 -132.
AMA StyleDapeng Jing, Tiantao Zhang, Shuxiong Bai, Kanglai He, Sivaprasath Prabu, Zhenying Wang. Artificial diet development for mass rearing and its effect on the reproduction of yellow peach moth, Conogethes punctiferalis (Guenée). Entomological Research. 2021; 51 (3):127-132.
Chicago/Turabian StyleDapeng Jing; Tiantao Zhang; Shuxiong Bai; Kanglai He; Sivaprasath Prabu; Zhenying Wang. 2021. "Artificial diet development for mass rearing and its effect on the reproduction of yellow peach moth, Conogethes punctiferalis (Guenée)." Entomological Research 51, no. 3: 127-132.
Transgenic plants expressing insecticidal proteins from the Bacillus thuringiensis (Bt) have provided an effective way to control target pests. However, the toxicity of Bt proteins against yellow peach moth (YPM), Conogethes punctiferalis (Guenée), one of the most serious maize pests in China, has not received much study. Therefore, we performed diet-overlay bioassays to evaluate the insecticidal activities of Cry1Ab, Cry1Ac, Cry1Fa, Cry1Ah, Cry1Ie, Cry2Aa, and Vip3Aa19, as well as the interaction between Cry1-Class, Cry2Aa, and Vip3Aa19 against YPM. Results showed that the LC50 values ranged from 1.08 to 178.12 ng/cm2 (protein/diet). Among these proteins, Cry1Ab and Cry1Ac had lower LC50 values and LC90 values. In YPM bioassays, the combinations of Cry2Aa with Cry1Ac, Cry1Ie, and Cry1Ab showed antagonism while a mixture of Cry2Aa with Cry1Fa and Cry1Ah exhibited synergism. When Vip3Aa19 was combined with Cry proteins, all combinations interacted positively, with variation in synergistic factors (SF). Three ratios 1:1, 1:2, and 2:1 of Cry1Ah and Vip3Aa19 protein combination showed SF values of 5.20, 5.63, and 8.98, respectively. These findings can be applied in the establishment of new pyramided transgenic crops with suitable candidates as well as in resistance management strategies.
Su Mon Shwe; Yueqin Wang; Zupeng Gao; Xue Li; Shen Liu; Shuxiong Bai; Tiantao Zhang; Kanglai He; Zhenying Wang. Toxicity of Cry1-Class, Cry2Aa, and Vip3Aa19 Bt proteins and their interactions against yellow peach Moth, Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae). Journal of Invertebrate Pathology 2020, 178, 107507 .
AMA StyleSu Mon Shwe, Yueqin Wang, Zupeng Gao, Xue Li, Shen Liu, Shuxiong Bai, Tiantao Zhang, Kanglai He, Zhenying Wang. Toxicity of Cry1-Class, Cry2Aa, and Vip3Aa19 Bt proteins and their interactions against yellow peach Moth, Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae). Journal of Invertebrate Pathology. 2020; 178 ():107507.
Chicago/Turabian StyleSu Mon Shwe; Yueqin Wang; Zupeng Gao; Xue Li; Shen Liu; Shuxiong Bai; Tiantao Zhang; Kanglai He; Zhenying Wang. 2020. "Toxicity of Cry1-Class, Cry2Aa, and Vip3Aa19 Bt proteins and their interactions against yellow peach Moth, Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae)." Journal of Invertebrate Pathology 178, no. : 107507.
Niemann-Pick type C2 (NPC2) proteins in arthropods have been extensively differentiated and possibly duplicated according to environmental conditions and are probable to have different functions. The participation of NPC2 proteins in chemical communication in arthropods brings new objectives in environmental-friendly strategies for pest population control. In this study, NPC2 gene in Macrocentrus cingulum (McinNPC2) was newly identified by rapid amplification cDNA ends (RACE) technology. McinNPC2 amino acid sequence alignment with other representative NPC2 annotates to evaluate the highly conserved consensus amino acids, but with odorant binding proteins in M. cingulum show that only one consensus amino acid. Primary six-cysteine structures that are same to odorant binding proteins in M. cingulum were observed in McinNPC2. Phylogenetic analysis of McinNPC2 indicated that the nearest monophyletic group forming one clade with high posterior probability values clusters as Cyphomyrmex costatus (CcosNPC2) whereas the nearest evolutionary relation group as some odorant binding proteins. Moreover, quantitative real-time PCR (qPCR) measurements show that the McinNPC2 gene expression level in various tissues of the female is significantly and ubiquitously higher than in male, whereas the highest expression level in female antennae. We further explore the binding characterization of recombinant McinNPC2 to candidate odor molecules and did the modeling and docking simulations. The results showed ligands binding specificity and docking tests results indicate that β-ionone, an aroma compound commonly found in essential oils, can strongly bind with McinNPC2. In conclusion, we proposed that McinNPC2 may be involved in chemical communication and play roles in perception of plant volatiles.
Kyaw Lin Maung; Dapeng Jing; Tiantao Zhang; Sivaprasath Prabu; Kanglai He; Shuxiong Bai; Zhenying Wang. Molecular identification and functional analysis of Niemann-Pick type C2 protein in Macrocentrus cingulum Brischke (Hymenoptera: Braconidae). Journal of Asia-Pacific Entomology 2020, 24, 7 -14.
AMA StyleKyaw Lin Maung, Dapeng Jing, Tiantao Zhang, Sivaprasath Prabu, Kanglai He, Shuxiong Bai, Zhenying Wang. Molecular identification and functional analysis of Niemann-Pick type C2 protein in Macrocentrus cingulum Brischke (Hymenoptera: Braconidae). Journal of Asia-Pacific Entomology. 2020; 24 (1):7-14.
Chicago/Turabian StyleKyaw Lin Maung; Dapeng Jing; Tiantao Zhang; Sivaprasath Prabu; Kanglai He; Shuxiong Bai; Zhenying Wang. 2020. "Molecular identification and functional analysis of Niemann-Pick type C2 protein in Macrocentrus cingulum Brischke (Hymenoptera: Braconidae)." Journal of Asia-Pacific Entomology 24, no. 1: 7-14.
Ostrinia furnacalis, is the major pest of maize causing significant yield losses. So far, many approaches have been used to increase the virulence of entomopathogenic fungal isolates. The current study is an attempt to estimate synergistic effect of Beauveria bassiana and Trichoderma asperellum in order to explore larval immune response through RNA sequencing and differentially expression analysis. In vivo synergism was examined in seven proportions (B. bassiana: T. asperellum = 1:1, 1:2, 1:3, 1:4, 4:1, 3:1, 2:1) and in the in vitro case, two inoculation methods were applied: seed coating and soil drenching. Results revealed significant decrease in plant damage and high larval mortality in fungal treatments. Fungal isolates mediated the plant defense by increasing proline, superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO) and protease activities. Seed coating method was proved to be the most effective in case of maize endophytic colonization. In total, 59 immune-related differentially expressed genes DEGs were identified including, cytochrome P450, heat shock protein, ABC transporter, cadherin, peptidoglycan recognition protein (PGRP), cuticlular protein, etc. Further, transcriptomic response was confirmed by qRT-PCR. Our results concluded that, coculture of B. bassiana and T. asperellum has the synergistic potential to suppress the immune response of O. furnacalis and can be used as sustainable approach to induce plant resistance through activation of defense-related enzymes.
RaufA Batool; Muhammad Umer; Yangzhou Wang; Kanglai He; Tiantao Zhang; Shuxiong Bai; Yang Zhi; Jie Chen; Zhenying Wang. Synergistic Effect of Beauveria bassiana and Trichoderma asperellum to Induce Maize (Zea mays L.) Defense against the Asian Corn Borer, Ostrinia furnacalis (Lepidoptera, Crambidae) and Larval Immune Response. International Journal of Molecular Sciences 2020, 21, 8215 .
AMA StyleRaufA Batool, Muhammad Umer, Yangzhou Wang, Kanglai He, Tiantao Zhang, Shuxiong Bai, Yang Zhi, Jie Chen, Zhenying Wang. Synergistic Effect of Beauveria bassiana and Trichoderma asperellum to Induce Maize (Zea mays L.) Defense against the Asian Corn Borer, Ostrinia furnacalis (Lepidoptera, Crambidae) and Larval Immune Response. International Journal of Molecular Sciences. 2020; 21 (21):8215.
Chicago/Turabian StyleRaufA Batool; Muhammad Umer; Yangzhou Wang; Kanglai He; Tiantao Zhang; Shuxiong Bai; Yang Zhi; Jie Chen; Zhenying Wang. 2020. "Synergistic Effect of Beauveria bassiana and Trichoderma asperellum to Induce Maize (Zea mays L.) Defense against the Asian Corn Borer, Ostrinia furnacalis (Lepidoptera, Crambidae) and Larval Immune Response." International Journal of Molecular Sciences 21, no. 21: 8215.
Mating and oviposition behavior can change the gene expression levels in insect antennae, especially for pheromone-binding protein (PBP) genes. In this study, we observed the development of the ovaries in the yellow peach moth, Conogethes punctiferalis (Guenée), from its early emergence to full maturity. Sex pheromones in the sex gland reached their highest quantity and influenced the expression of the PBP2 gene in male antennae. Furthermore, the expression of PBP genes was measured after mating and oviposition. The result showed that mating resulted in an increased expression of the PBP2 gene in male antennae, while the expression of the PBP1 gene increased in female antennae, indicating that PBP genes might play different functions in different sexes. Compared with females that had not mated, the PBP1 and PBP5 genes were up-regulated in female antennae after mating or oviposition. Altogether, the PBP genes were regulated by sex pheromone release, mating or oviposition, suggesting that these genes may play some critical roles in behaviors associated with reproduction.
Dapeng Jing; Tiantao Zhang; Sivaprasath Prabu; Shuxiong Bai; Kanglai He; Junbo Luan; Zhenying Wang. PBP genes regulated by the development of the ovaries, sex pheromone release, mating and oviposition behavior in Conogethes punctiferalis (Guenée). Chemoecology 2020, 31, 1 -9.
AMA StyleDapeng Jing, Tiantao Zhang, Sivaprasath Prabu, Shuxiong Bai, Kanglai He, Junbo Luan, Zhenying Wang. PBP genes regulated by the development of the ovaries, sex pheromone release, mating and oviposition behavior in Conogethes punctiferalis (Guenée). Chemoecology. 2020; 31 (1):1-9.
Chicago/Turabian StyleDapeng Jing; Tiantao Zhang; Sivaprasath Prabu; Shuxiong Bai; Kanglai He; Junbo Luan; Zhenying Wang. 2020. "PBP genes regulated by the development of the ovaries, sex pheromone release, mating and oviposition behavior in Conogethes punctiferalis (Guenée)." Chemoecology 31, no. 1: 1-9.
Yunhe Li; Zhenying Wang; Jörg Romeis. Managing the Invasive Fall Armyworm through Biotech Crops: A Chinese Perspective. Trends in Biotechnology 2020, 39, 105 -107.
AMA StyleYunhe Li, Zhenying Wang, Jörg Romeis. Managing the Invasive Fall Armyworm through Biotech Crops: A Chinese Perspective. Trends in Biotechnology. 2020; 39 (2):105-107.
Chicago/Turabian StyleYunhe Li; Zhenying Wang; Jörg Romeis. 2020. "Managing the Invasive Fall Armyworm through Biotech Crops: A Chinese Perspective." Trends in Biotechnology 39, no. 2: 105-107.
The fall armyworm (FAW, Spodoptera frugiperda) is an important polyphagous insect pest in many crops. This highly invasive pest species originates from the Americas and recently spread rapidly across more than 100 countries worldwide. It poses a major threat to food security in a number of developing countries due to its rapid spread and distinctive ability to inflict widespread damage across multiple crops. Chemical insecticides are used as the main management strategy to control FAW in many parts of the world, particularly in the recently invaded areas in Africa and Asia. However, concerns over the adverse effects on environment and humans, and the development of resistance against insecticides have intensified efforts to develop alternatives that are effective and low-risk, while at the same time cost effective. Given that microbials are generally considered desirable options for pest management, this review compiles information on microbials in all phases of their development including entomopathogenic fungi, entomopathogenic nematodes, bacteria and baculoviruses, with a special focus on their efficacy against FAW. In addition, combinations of microbial agents and also mixtures with compatible insecticides for improved control of FAW are reviewed. The findings are discussed in light of improving management programs of FAW
Jingfei Guo; Shengyong Wu; Feng Zhang; Chaolong Huang; Kanglai He; Dirk Babendreier; Zhenying Wang. Prospects for microbial control of the fall armyworm Spodoptera frugiperda: a review. BioControl 2020, 65, 647 -662.
AMA StyleJingfei Guo, Shengyong Wu, Feng Zhang, Chaolong Huang, Kanglai He, Dirk Babendreier, Zhenying Wang. Prospects for microbial control of the fall armyworm Spodoptera frugiperda: a review. BioControl. 2020; 65 (6):647-662.
Chicago/Turabian StyleJingfei Guo; Shengyong Wu; Feng Zhang; Chaolong Huang; Kanglai He; Dirk Babendreier; Zhenying Wang. 2020. "Prospects for microbial control of the fall armyworm Spodoptera frugiperda: a review." BioControl 65, no. 6: 647-662.
Evolution of insect resistance to Bt toxins challenges the use of Cry toxins to control agricultural pests. In lepidopterans, Cry toxin affinity towards multiple midgut epithelial receptors has become a matter of dispute. Cry1Ah toxin-binding proteins were identified in the larval midgut of susceptible (ACB-BtS) and resistant (ACB-AhR) strains of the Asian corn borer (ACB). A pull-down assay was performed using biotinylated Cry1Ah toxin, and the binding proteins were identified by employing liquid chromatography–tandem mass spectrometry (LC-MS/MS). This study aimed to find the binding consistency of the midgut epithelial protein to the Cry1Ah toxin. The binding proteins from different fractions of SDS-PAGE showed a different pattern. We observed an isoform of prophenoloxidase PPO1b (UniProt Acc No. A0A1Q1MKI0), which was found only in the ACB-AhR fractions. Prophenoloxidase (proPO) is an extraordinary defense molecule activated in insect species during pathogen invasion and the wound healing process. Importantly, this prophenoloxidase might have direct/indirect interaction with the Cry1Ah toxin. Our data also suggest that factors like techniques, enrichment of binding proteins in the sample and the reversible and irreversible nature of the brush border membrane vesicles (BBMVs) to Cry toxins could cause the inconsistency in the protein–protein interactions. Moreover, inside the larva midgut, the influence of the Cry toxins under physiological conditions might be different from the laboratory procedures.
Sivaprasath Prabu; Muhammad Shabbir; Zhenying Wang; Kanglai He. Analysis of Cry1Ah Toxin-Binding Reliability to Midgut Membrane Proteins of the Asian Corn Borer. Toxins 2020, 12, 418 .
AMA StyleSivaprasath Prabu, Muhammad Shabbir, Zhenying Wang, Kanglai He. Analysis of Cry1Ah Toxin-Binding Reliability to Midgut Membrane Proteins of the Asian Corn Borer. Toxins. 2020; 12 (6):418.
Chicago/Turabian StyleSivaprasath Prabu; Muhammad Shabbir; Zhenying Wang; Kanglai He. 2020. "Analysis of Cry1Ah Toxin-Binding Reliability to Midgut Membrane Proteins of the Asian Corn Borer." Toxins 12, no. 6: 418.
Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), is a major polyphagous pest with the potential to seriously damage various crops. A better understanding of FAW's performance on different hosts may help to predict which plants will be attacked when the preferred host is absent, and facilitate the development of effective pest management practices. We compared the larval performance of FAW fed on maize with that of FAW fed on potato and tobacco, which are important crops in China, using an age‐stage two‐sex life table and adult female oviposition preference experiments. In cage experiments with potato, tobacco, or maize as the host, FAW reared on maize exhibited the strongest performance with shorter developmental time in the larval stage, longer longevity, and a higher reproductive rate in adults. Females oviposited on maize in preference to potato or tobacco. Compared with larvae fed on maize, those fed on potato and tobacco exhibited significantly lower survival, with only 31.61% and 8.13% developing to the adult stage, respectively. Several life table parameters, including the mean generation time (T ), net reproductive rate (R 0), finite rate of increase (λ ), and intrinsic rate of natural increase (r m) were negatively affected in FAW fed on potato and tobacco. Our results support the preference–performance hypothesis, i.e., that herbivore females maximize fitness by choosing host plants associated with strong larval performance. Although larvae and adults performed poorly on potato and tobacco, their offspring will be capable of establishing populations on them, posing a potential threat to these crops in China. This article is protected by copyright. All rights reserved
Jing‐Fei Guo; Meng‐Di Zhang; Zu‐Peng Gao; Deng‐Jie Wang; Kang‐Lai He; Zhen‐Ying Wang. Comparison of larval performance and oviposition preference of Spodoptera frugiperda among three host plants: Potential risks to potato and tobacco crops. Insect Science 2020, 28, 602 -610.
AMA StyleJing‐Fei Guo, Meng‐Di Zhang, Zu‐Peng Gao, Deng‐Jie Wang, Kang‐Lai He, Zhen‐Ying Wang. Comparison of larval performance and oviposition preference of Spodoptera frugiperda among three host plants: Potential risks to potato and tobacco crops. Insect Science. 2020; 28 (3):602-610.
Chicago/Turabian StyleJing‐Fei Guo; Meng‐Di Zhang; Zu‐Peng Gao; Deng‐Jie Wang; Kang‐Lai He; Zhen‐Ying Wang. 2020. "Comparison of larval performance and oviposition preference of Spodoptera frugiperda among three host plants: Potential risks to potato and tobacco crops." Insect Science 28, no. 3: 602-610.
Black cutworm (BCW), Agrotis ipsilon (Hufnagel), is an occasional pest of maize that can cause considerable economic loss and injury to corn seedlings. This research mainly assessed the susceptibility of BCW neonates to 11 Bt toxins (Cry1Ab, Cry1Ac, Cry1Ah, Cry1F, Cry1Ie, Cry1B, Cry2Aa, Vip3_ch1, Vip3_ch4, Vip3Ca2, Vip3Aa19) by exposing neonates to an artificial diet containing Bt toxins and evaluated the efficacy of three transgenic maize events (C008, C009, C010) expressing Vip3Aa19 toxin against BCW. The toxin-diet bioassay data indicated that Vip3Aa19 protein (LC50 = 0.43 μg/g) was the most active against BCW. Chimeric protein Vip3_ch1 (LC50 = 5.53 μg/g), Cry1F (LC50 = 83.62 μg/g) and Cry1Ac (LC50 = 184.77 μg/g) were less toxic. BCW was very tolerant to the other Bt toxins tested, with LC50 values more than 200 μg/g. Greenhouse studies were conducted with artificial infestations at the whorl stage by placing second-instar BCW larvae into whorl leaf and the fourth-instar larvae at the base of maize seedings. These results suggest that these transgenic maize events expressing Vip3Aa19 can provide effective control for BCW.
Xiaorui Yan; Junjiao Lu; Meifeng Ren; Yin He; Yueqin Wang; Zhenying Wang; Kanglai He. Insecticidal Activity of 11 Bt toxins and 3 Transgenic Maize Events Expressing Vip3Aa19 to Black Cutworm, Agrotis ipsilon (Hufnagel). Insects 2020, 11, 208 .
AMA StyleXiaorui Yan, Junjiao Lu, Meifeng Ren, Yin He, Yueqin Wang, Zhenying Wang, Kanglai He. Insecticidal Activity of 11 Bt toxins and 3 Transgenic Maize Events Expressing Vip3Aa19 to Black Cutworm, Agrotis ipsilon (Hufnagel). Insects. 2020; 11 (4):208.
Chicago/Turabian StyleXiaorui Yan; Junjiao Lu; Meifeng Ren; Yin He; Yueqin Wang; Zhenying Wang; Kanglai He. 2020. "Insecticidal Activity of 11 Bt toxins and 3 Transgenic Maize Events Expressing Vip3Aa19 to Black Cutworm, Agrotis ipsilon (Hufnagel)." Insects 11, no. 4: 208.
Background Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Results Totally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis. The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis. Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues. Conclusions Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis, which might be supportive for pest management studies in future.
Dapeng Jing; Tiantao Zhang; Shuxiong Bai; Kanglai He; Sivaprasath Prabu; Junbo Luan; Zhenying Wang. Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae). BMC Genomics 2020, 21, 244 -13.
AMA StyleDapeng Jing, Tiantao Zhang, Shuxiong Bai, Kanglai He, Sivaprasath Prabu, Junbo Luan, Zhenying Wang. Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae). BMC Genomics. 2020; 21 (1):244-13.
Chicago/Turabian StyleDapeng Jing; Tiantao Zhang; Shuxiong Bai; Kanglai He; Sivaprasath Prabu; Junbo Luan; Zhenying Wang. 2020. "Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)." BMC Genomics 21, no. 1: 244-13.
Conogethes pinicolalis (Lepidoptera: Crambidae), a major pine pest, possesses a sensitive olfactory system to locate its host. Pheromone binding proteins (PBPs) and general odorant binding proteins (GOBPs) are two types of proteins involved in the process. In this work, we used phylogenetic analysis, gene expression, fluorescence competitive binding assay, and molecular docking to characterize PBPs and GOBPs in C. pinicolalis. The phylogenetic unrooted tree revealed the C. pinicolalis GOBPs and PBPs amino acid sequences showed very close relation with Conogethes punctiferalis (yellow peach moth). Meanwhile, both the PBPs and GOBPs were specifically expressed in the antennae. Binding affinities of PBPs and GOBPs to 19 volatile compounds were tested. PBP2 shows the strongest binding to E10-16: Ald with a Ki value of 0.28/1.66 μM; GOBP1 to Z10-16:Ald with a Ki value of 3.11/4.24 μM. Furthermore, molecular docking reveals potential active sites in PBP2 and GOBP1 to interact with most of the tested volatiles. Finally, we demonstrate that PBP2 and GOBP1 are the dominant genes in their respective families using several different assays. In conclusion, PBP2 and GOBP1 genes may play similar roles in detecting and transporting sex pheromones and host plant volatiles in C. pinicolalis.
Dapeng Jing; Tiantao Zhang; Sivaprasath Prabu; Shuxiong Bai; Kanglai He; Zhenying Wang. Molecular characterization and volatile binding properties of pheromone binding proteins and general odorant binding proteins in Conogethes pinicolalis (Lepidoptera: Crambidae). International Journal of Biological Macromolecules 2020, 146, 263 -272.
AMA StyleDapeng Jing, Tiantao Zhang, Sivaprasath Prabu, Shuxiong Bai, Kanglai He, Zhenying Wang. Molecular characterization and volatile binding properties of pheromone binding proteins and general odorant binding proteins in Conogethes pinicolalis (Lepidoptera: Crambidae). International Journal of Biological Macromolecules. 2020; 146 ():263-272.
Chicago/Turabian StyleDapeng Jing; Tiantao Zhang; Sivaprasath Prabu; Shuxiong Bai; Kanglai He; Zhenying Wang. 2020. "Molecular characterization and volatile binding properties of pheromone binding proteins and general odorant binding proteins in Conogethes pinicolalis (Lepidoptera: Crambidae)." International Journal of Biological Macromolecules 146, no. : 263-272.
Insects recognize odorous compounds using sensory neurons organized in olfactory sensilla. The process odor detection in insects requires an ensemble of proteins, including odorant binding proteins, olfactory receptors, and odor degrading enzymes; each of them are encoded by multigene families. Most functional proteins seem to be broadly tuned, responding to multiple chemical compounds with different, but mostly quite similar structures. Based on the hypothesis that insects recognize host volatiles by means of general odorant binding proteins (GOBPs), the current study aimed to characterize GOBPs of the yellow peach moth, Conogethes punctiferalis (Guenée). In oviposition preference tests, it was found that the yellow peach moth preferred volatiles from Prunus persica (peach) in finding their host plant. Exposure of the moth to volatiles from peaches affected the expression level of GOBP genes. Binding affinity of GOBPs from yellow peach moth was assessed for 16 host plant volatiles and 2 sex pheromones. The fluorescence ligand-binding assays revealed highest affinities for hexadecanal, farnesol, and limonene with KD values of 0.55 ± 0.08, 0.35 ± 0.04, and 1.54 ± 0.39, respectively. The binding sites of GOBPs from yellow peach moth were predicted using homology modeling and characterized using molecular docking approaches. The results indicated the best binding affinity of both GOBP1 and GOBP2 for farnesol, with scores of −7.4 and −8.5 kcal/mol. Thus, GOBPs may play an important role in the process of finding host plants.
Dapeng Jing; Tiantao Zhang; Shuxiong Bai; Sivaprasath Prabu; Kanglai He; Youssef Dewer; Zhenying Wang; Jing; Bai; He; Wang. GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, Conogethes punctiferalis (Lepidoptera: Crambidae). Insects 2019, 10, 302 .
AMA StyleDapeng Jing, Tiantao Zhang, Shuxiong Bai, Sivaprasath Prabu, Kanglai He, Youssef Dewer, Zhenying Wang, Jing, Bai, He, Wang. GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, Conogethes punctiferalis (Lepidoptera: Crambidae). Insects. 2019; 10 (9):302.
Chicago/Turabian StyleDapeng Jing; Tiantao Zhang; Shuxiong Bai; Sivaprasath Prabu; Kanglai He; Youssef Dewer; Zhenying Wang; Jing; Bai; He; Wang. 2019. "GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, Conogethes punctiferalis (Lepidoptera: Crambidae)." Insects 10, no. 9: 302.
Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) have revolutionized pest control, but the benefits of this approach have been reduced by the evolution of resistance in pests. The widely adopted 'pyramid strategy' for delaying resistance entails transgenic crops producing two or more distinct toxins that kill the same pest. The limited experimental evidence supporting this strategy comes primarily from a model system under ideal conditions. Here we tested the pyramid strategy under nearly worst-case conditions, including some cross-resistance between the toxins in the pyramid. In a laboratory selection experiment with an artificial diet, we used Bt toxins Cry1Ab, Cry1F, and Cry1Ie singly or in pairs against Ostrinia furnacalis, one of the most destructive pests of corn in Asia. Under the conditions evaluated, pairs of toxins did not consistently delay the evolution of resistance relative to single toxins.
Yueqin Wang; Yudong Quan; Jing Yang; Changlong Shu; Zhenying Wang; Jie Zhang; Angharad M. R. Gatehouse; Bruce E. Tabashnik; Kanglai He. Evolution of Asian Corn Borer Resistance to Bt Toxins Used Singly or in Pairs. Toxins 2019, 11, 461 .
AMA StyleYueqin Wang, Yudong Quan, Jing Yang, Changlong Shu, Zhenying Wang, Jie Zhang, Angharad M. R. Gatehouse, Bruce E. Tabashnik, Kanglai He. Evolution of Asian Corn Borer Resistance to Bt Toxins Used Singly or in Pairs. Toxins. 2019; 11 (8):461.
Chicago/Turabian StyleYueqin Wang; Yudong Quan; Jing Yang; Changlong Shu; Zhenying Wang; Jie Zhang; Angharad M. R. Gatehouse; Bruce E. Tabashnik; Kanglai He. 2019. "Evolution of Asian Corn Borer Resistance to Bt Toxins Used Singly or in Pairs." Toxins 11, no. 8: 461.
Climate change is a major environmental concern and is directly related to the increasing concentrations of greenhouse gases. The increase in concentrations of atmospheric carbon dioxide (CO2), not only affects plant growth and development, but also affects the emission of plant organic volatile compounds (VOCs). Changes in the plant odor profile may affect the plant-insect interactions, especially the behavior of herbivorous insects. In this study, we compared the foraging behavior of corn leaf aphid (Rhopalosiphum maidis) on barley (Hordeum vulgare L.) seedlings grown under contrasted CO2 concentrations. During the dual choice bioassays, the winged and wingless aphids were more attracted by the VOCs of barley seedlings cultivated under ambient CO2 concentrations (aCO2; 450 ppm) than barley seedlings cultivated under elevated CO2 concentrations (eCO2; 800 ppm), nymphs were not attracted by the VOCs of eCO2 barley seedlings. Then, volatile compositions from 14-d-old aCO2 and eCO2 barley seedlings were investigated by GC-MS. While 16 VOCs were identified from aCO2 barley seedlings, only 9 VOCs were found from eCO2 barley seedlings. At last, we discussed the potential role of these chemicals observed during choice bioassays. Our findings lay foundation for functional response of corn leaf aphid under climate change through host plant modifications.
Yu Chen; Clément Martin; Junior Corneille Fingu Mabola; François Verheggen; Zhenying Wang; Kanglai He; Frederic Francis. Effects of Host Plants Reared under Elevated CO2 Concentrations on the Foraging Behavior of Different Stages of Corn Leaf Aphids Rhopalosiphum maidis. Insects 2019, 10, 182 .
AMA StyleYu Chen, Clément Martin, Junior Corneille Fingu Mabola, François Verheggen, Zhenying Wang, Kanglai He, Frederic Francis. Effects of Host Plants Reared under Elevated CO2 Concentrations on the Foraging Behavior of Different Stages of Corn Leaf Aphids Rhopalosiphum maidis. Insects. 2019; 10 (6):182.
Chicago/Turabian StyleYu Chen; Clément Martin; Junior Corneille Fingu Mabola; François Verheggen; Zhenying Wang; Kanglai He; Frederic Francis. 2019. "Effects of Host Plants Reared under Elevated CO2 Concentrations on the Foraging Behavior of Different Stages of Corn Leaf Aphids Rhopalosiphum maidis." Insects 10, no. 6: 182.
The fall armyworm, Spodoptera frugiperda, is a species native to the Americas and has spread to many countries in Africa and Asia in recent years. Proactive actions for potential invasion of S. frugiperda to China coordinated by government agencies and agricultural extension systems resulted in timely detection in January 2019 in Yunnan Province neighboring to Myanmar. The extensive monitoring in southern provinces of China since February 2019 resulted in dynamic tracking of the S. frugiperda spreading to 13 provincial regions in China within four months by May 10, 2019, which is crucial for timely management actions in the fields. The first detections of S. frugiperda (corn strain) in China were confirmed using cytochrome oxidase subunit 1 (CO1) and triosephosphate isomerase (Tpi) genes molecular marker method. In addition to S. frugiperda, larvae of three other noctuid species with similar morphological appearance (S. litura, S. exigua and Mythimna separata) can occur simultaneously and cause similar damage in cornfield in southern China. Thus, we can use both morphological and molecular marker methods to compare larval stages of four noctuid species. Further, we discussed the risk of potential spread of invasive S. frugiperda to other regions and impact on corn production in China. This article is protected by copyright. All rights reserved
Da‐Peng Jing; Jing‐Fei Guo; Yu‐Ying Jiang; Jian‐Zhou Zhao; Amit Sethi; Kang‐Lai He; Zhen‐Ying Wang. Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques. Insect Science 2019, 27, 780 -790.
AMA StyleDa‐Peng Jing, Jing‐Fei Guo, Yu‐Ying Jiang, Jian‐Zhou Zhao, Amit Sethi, Kang‐Lai He, Zhen‐Ying Wang. Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques. Insect Science. 2019; 27 (4):780-790.
Chicago/Turabian StyleDa‐Peng Jing; Jing‐Fei Guo; Yu‐Ying Jiang; Jian‐Zhou Zhao; Amit Sethi; Kang‐Lai He; Zhen‐Ying Wang. 2019. "Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques." Insect Science 27, no. 4: 780-790.