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In many ecosystems, the zooplankton community has been pressured simultaneously by microplastic pollution and alterations resulting from global climate changes. The potential influence of light intensity rise (from 10,830 lx to 26,000 lx) and water temperature rise (from 20 °C to 25 °C) on the long term-toxicity of microplastics (MPs) to Daphnia magna were investigated. Three 21-day laboratory bioassays with model MPs (1–5 μm diameter) were carried out at (i) 20 °C/10830 lx, (ii) 20 °C/26000 lx, and (iii) 25 °C/10830 lx. In each bioassay, one control (no MPs) and three MP concentrations (0.04, 0.09, 0.19 mg/L) were tested. In all the bioassays, MPs caused parental and juvenile mortality, and reduced the somatic growth, reproduction and population growth rate. The MP EC50s on living offspring (95% confidence interval within brackets) were 0.146 mg/L (0.142–0.151 mg/L) at 20 °C/10830 lx, 0.102 mg/L (0.099–0.105 mg/L) at 20 °C/26000 lx, and 0.101 mg/L (0.098–0.104 mg/L) at 25 °C/10830 lx. Relatively to the respective control group, 0.19 mg/L of MPs decreased the mean of the population growth rate by 27% at 20 °C/10830 lx, 38% at 20 °C/26000 lx and 59% at 25 °C/10830 lx. Based on the population growth rate and in relation to 20 °C/10830 lx (control, no MPs), the interaction between increased light intensity (26,000 lx) and MPs was synergism (at all the MP concentrations tested). The interaction between water temperature rise (25 °C) and MPs was antagonism at 0.04 mg/L of MPs and synergism at 0.09 and 0.19 mg/L of MPs. In the present scenario of climate changes and global MP pollution such findings raise high concern because zooplankton communities are crucial for aquatic biodiversity conservation, ecosystem functioning and services provided to humans. Further studies on the combined effects of MPs, other common pollutants, and alterations due to climate changes are needed.
Lúcia Guilhermino; Alexandra Martins; Sara Cunha; José O. Fernandes. Long-term adverse effects of microplastics on Daphnia magna reproduction and population growth rate at increased water temperature and light intensity: Combined effects of stressors and interactions. Science of The Total Environment 2021, 784, 147082 .
AMA StyleLúcia Guilhermino, Alexandra Martins, Sara Cunha, José O. Fernandes. Long-term adverse effects of microplastics on Daphnia magna reproduction and population growth rate at increased water temperature and light intensity: Combined effects of stressors and interactions. Science of The Total Environment. 2021; 784 ():147082.
Chicago/Turabian StyleLúcia Guilhermino; Alexandra Martins; Sara Cunha; José O. Fernandes. 2021. "Long-term adverse effects of microplastics on Daphnia magna reproduction and population growth rate at increased water temperature and light intensity: Combined effects of stressors and interactions." Science of The Total Environment 784, no. : 147082.
The effects of polystyrene nanoplastics (NP), lithium (Li), and their mixtures were investigated using the exotic invasive bivalve Corbicula fluminea as test organism for ethical reasons. In a 96 h laboratory bioassay, groups of bivalves were exposed to water (control), 0.8 mg/L NP, 3.2 mg/L NP, 1 mg/L Li, 4 mg/L Li, 0.8 mg/L NP + 1 mg/L Li (Mix1) or 3.2 mg/L NP + 4 mg/L Li (Mix2). Effect criteria were filtration rate (FR), lipid peroxidation levels (LPO), and the activity of the enzymes cholinesterases (ChE), isocitrate dehydrogenase (IDH), octopine dehydrogenase, glutathione S-transferases and glutathione reductase. Bivalves exposed to NP alone or in mixture had IDH activity inhibition (22%), and LPO reduced by 33%. Bivalves exposed to Li alone or in mixture had 22% of ChE inhibition. Bivalves exposed to Mix2 had FR induction (twofold). These results suggest that the NP tested may decrease the cellular energy production by anaerobic pathways despite providing some reduction of lipid oxidative damage that Li has anticholinergic effects, and that NP and Li may cause toxicological interactions in bivalves exposed simultaneously to the two substances. Further studies are needed to understand potential toxicological interactions between NP and Li under different abiotic conditions and exposure periods.
Rafaela S. Costa; Patrícia Oliveira; Lúcia Guilhermino. Effects of Nanoplastics, Lithium, and Their Mixtures on Corbicula fluminea: Preliminary Findings. Plant-Microbes-Engineered Nano-particles (PM-ENPs) Nexus in Agro-Ecosystems 2021, 279 -282.
AMA StyleRafaela S. Costa, Patrícia Oliveira, Lúcia Guilhermino. Effects of Nanoplastics, Lithium, and Their Mixtures on Corbicula fluminea: Preliminary Findings. Plant-Microbes-Engineered Nano-particles (PM-ENPs) Nexus in Agro-Ecosystems. 2021; ():279-282.
Chicago/Turabian StyleRafaela S. Costa; Patrícia Oliveira; Lúcia Guilhermino. 2021. "Effects of Nanoplastics, Lithium, and Their Mixtures on Corbicula fluminea: Preliminary Findings." Plant-Microbes-Engineered Nano-particles (PM-ENPs) Nexus in Agro-Ecosystems , no. : 279-282.
Luís Gabriel A. Barboza; Sara C. Cunha; Carolina Monteiro; José O. Fernandes; Lúcia Guilhermino. Corrigendum to “Bisphenol A and its analogs in muscle and liver of fish from the North East Atlantic Ocean in relation to microplastic contamination. Exposure and risk to human consumers” [J. Hazard. Mater. 393 (2020) 122419]. Journal of Hazardous Materials 2021, 415, 125654 .
AMA StyleLuís Gabriel A. Barboza, Sara C. Cunha, Carolina Monteiro, José O. Fernandes, Lúcia Guilhermino. Corrigendum to “Bisphenol A and its analogs in muscle and liver of fish from the North East Atlantic Ocean in relation to microplastic contamination. Exposure and risk to human consumers” [J. Hazard. Mater. 393 (2020) 122419]. Journal of Hazardous Materials. 2021; 415 ():125654.
Chicago/Turabian StyleLuís Gabriel A. Barboza; Sara C. Cunha; Carolina Monteiro; José O. Fernandes; Lúcia Guilhermino. 2021. "Corrigendum to “Bisphenol A and its analogs in muscle and liver of fish from the North East Atlantic Ocean in relation to microplastic contamination. Exposure and risk to human consumers” [J. Hazard. Mater. 393 (2020) 122419]." Journal of Hazardous Materials 415, no. : 125654.
The experienced view of stakeholders is a very valuable tool to build inclusive and reliable maritime spatial planning (MSP). Within this context, the present work assesses the potential and limitations for a further sustainable development of fishing and aquaculture activities, considering the Portuguese North Region as case study. The official strategies and legal framework drafted by Portugal in MSP issues were initially reviewed, with the corresponding management objectives identified. Official statistical data were used to show the current situation of regional fishery and aquaculture, while the perceptions of involved groups were collected by a methodology based in a multi-stakeholder survey and subsequent workshop. Taking into account the regional circumstances defined by a decreased fishing production (decline of 45.9% during the period of 2012–2019) and scarce aquaculture weight (≤1% in terms of national production in 2018), the stakeholders brought to light great difficulties on the part of public administration to implement official management objectives. The stakeholders also considered that conflicts between maritime activities are almost inexistent at present, even though they predicted future disagreements when new players intend to use maritime space. A positive response about a successful future for aquaculture was obtained from every group surveyed, although the specialized stakeholders pointed out severe limitations for a further development of both off-shore and extensive coastal aquaculture modalities. In conclusion, it seems evident there is the need for a fluent collaboration with the regional fishing stakeholder, particularly promoting synergies involving small scale fleets, in order to avoid future potential conflicts. Against the challenges and limitations posed by the aquaculture industry, promoting the intensive cultivation of high commercial value fish and new interest local species, when conducted under sustainable practices that add value to the harvested product, would be an interesting strategy to implement in our case study.
Salas-Leiton E.; Vieira L.R.; Guilhermino L.. Sustainable Fishing and Aquaculture Activities in the Atlantic Coast of the Portuguese North Region: Multi-Stakeholder Views as a Tool for Maritime Spatial Planning. Sustainability 2021, 13, 663 .
AMA StyleSalas-Leiton E., Vieira L.R., Guilhermino L.. Sustainable Fishing and Aquaculture Activities in the Atlantic Coast of the Portuguese North Region: Multi-Stakeholder Views as a Tool for Maritime Spatial Planning. Sustainability. 2021; 13 (2):663.
Chicago/Turabian StyleSalas-Leiton E.; Vieira L.R.; Guilhermino L.. 2021. "Sustainable Fishing and Aquaculture Activities in the Atlantic Coast of the Portuguese North Region: Multi-Stakeholder Views as a Tool for Maritime Spatial Planning." Sustainability 13, no. 2: 663.
The present study tested under laboratorial conditions the sorption of okadaic acid (OA), a lipophilic toxin produced by marine phytoplankton, onto 6-mm plastic circular fragments. Fragments (4 g L−1) of polyethylene terephthalate (PET), polypropylene (PP), expanded polystyrene (EPS) and non-expanded polystyrene (PS) were exposed for 96 h to seawater spiked with 10 ng mL−1 of OA. Results of the experiments showed a broad percentage of OA removed from the water by plastics after 48 h of exposure: 30 ± 5.1% (PET), 37 ± 9.5% (PP), 62 ± 7.1% (EPS) and 83 ± 1.9% (PS). Sorption appears to be highly influenced by polymer's characteristics, such as polarity and degree of crystallinity. Further studies are needed to clarify the effect of the contact area on sorption by expanded plastics. These results point to the plausible interaction of OA and plastics in coastal waters.
Sara T. Costa; Alisa Rudnitskaya; Carlos Vale; Lúcia Guilhermino; Maria João Botelho. Sorption of okadaic acid lipophilic toxin onto plastics in seawater. Marine Pollution Bulletin 2020, 157, 111322 .
AMA StyleSara T. Costa, Alisa Rudnitskaya, Carlos Vale, Lúcia Guilhermino, Maria João Botelho. Sorption of okadaic acid lipophilic toxin onto plastics in seawater. Marine Pollution Bulletin. 2020; 157 ():111322.
Chicago/Turabian StyleSara T. Costa; Alisa Rudnitskaya; Carlos Vale; Lúcia Guilhermino; Maria João Botelho. 2020. "Sorption of okadaic acid lipophilic toxin onto plastics in seawater." Marine Pollution Bulletin 157, no. : 111322.
In the present study, the levels of bisphenol A (BPA) and analagous compounds in muscle and liver of fish (Dicentrarchus labrax, Trachurus trachurus, Scomber colias) from the North East Atlantic Ocean were determined and the risk of their consumption by humans was assessed. The potential relationship between bisphenol concentrations and microplastic (MP) contamination of fish was also investigated. Fish from all the species had BPA in the liver and muscle, and bisphenol B (BPB) and bisphenol E (BPE) in the muscle. The highest concentration of BPA in the liver (302 ng/g dry weight - dw) was found in S. colias and the lowest one (5 ng/g dw) in T. trachurus. In the muscle, the bisphenol with the highest concentration was BPE in S. colias (272 ng/g dw). Fish with microplastics had significantly higher concentrations of bisphenols than fish where no microplastics were found, suggesting a relation between MP and bisphenol contamination in fish. In all species, the concentration of bisphenols was correlated with higher MP intake. Regarding human food safety, the estimated daily intake (EDI), target hazard quotient (THQ) and hazard index (HI) of bisphenols were higher than those established by the European Food Safety Authority suggesting hazardous risk for human consumers. These findings highlight the need of more research on fish contamination by MP and associated chemicals and inherent human food safety risks.
Luis Gabriel Antão Barboza; Sara C. Cunha; Carolina Monteiro; José O. Fernandes; Lúcia Guilhermino. Bisphenol A and its analogs in muscle and liver of fish from the North East Atlantic Ocean in relation to microplastic contamination. Exposure and risk to human consumers. Journal of Hazardous Materials 2020, 393, 122419 .
AMA StyleLuis Gabriel Antão Barboza, Sara C. Cunha, Carolina Monteiro, José O. Fernandes, Lúcia Guilhermino. Bisphenol A and its analogs in muscle and liver of fish from the North East Atlantic Ocean in relation to microplastic contamination. Exposure and risk to human consumers. Journal of Hazardous Materials. 2020; 393 ():122419.
Chicago/Turabian StyleLuis Gabriel Antão Barboza; Sara C. Cunha; Carolina Monteiro; José O. Fernandes; Lúcia Guilhermino. 2020. "Bisphenol A and its analogs in muscle and liver of fish from the North East Atlantic Ocean in relation to microplastic contamination. Exposure and risk to human consumers." Journal of Hazardous Materials 393, no. : 122419.
Corbicula fluminea and Dreissena polymorpha are two non-indigenous species (NIS), known to provoke biodiversity loss of the existent native communities and alterations in the ecosystem functioning structure. Both of these NIS have successfully founded well established populations in Lakes Maggiore and Garda (Northern Italy). Here, we evaluated the mitochondrial COI genetic diversity of C. fluminea and D. polymorpha populations from the aforementioned lakes. The COI gene analysis revealed one C. fluminea haplotype, belonging to the FW5 androgenetic invasive lineage. Two D. polymorpha haplotypes – LM1 and LM2 – were detected in Lake Maggiore. The D. polymorpha comparative phylogeographical haplotype analysis between Lake Maggiore and the retrieved COI data available from Lake Garda revealed that LM1 is the dominant haplotype in both populations, whereas LM2 a rare haplotype was only detected in Lake Maggiore. These findings contribute for a better understanding of the demographic history of these highly invasive species in these Italian lakes, thus suggesting that C. fluminea and D. polymorpha populations present a similar genetic pattern. The low genetic diversity detected in both of these bivalve populations seems to reflect a pre-existent low genetic pool prevenient from the introductory source(s).
Cidália Gomes; Tito Mendes; Rui Borges; Irene Guarneri; Ilaria Marchi; Lucia Guilhermino; Vitor Vasconcelos; Nicoletta Rita Riccardi; Agostinho Antunes. The genetic diversity of two invasive sympatric bivalves (Corbicula fluminea and Dreissena polymorpha) from Lakes Garda and Maggiore, Northern Italy. Journal of Great Lakes Research 2019, 46, 225 -229.
AMA StyleCidália Gomes, Tito Mendes, Rui Borges, Irene Guarneri, Ilaria Marchi, Lucia Guilhermino, Vitor Vasconcelos, Nicoletta Rita Riccardi, Agostinho Antunes. The genetic diversity of two invasive sympatric bivalves (Corbicula fluminea and Dreissena polymorpha) from Lakes Garda and Maggiore, Northern Italy. Journal of Great Lakes Research. 2019; 46 (1):225-229.
Chicago/Turabian StyleCidália Gomes; Tito Mendes; Rui Borges; Irene Guarneri; Ilaria Marchi; Lucia Guilhermino; Vitor Vasconcelos; Nicoletta Rita Riccardi; Agostinho Antunes. 2019. "The genetic diversity of two invasive sympatric bivalves (Corbicula fluminea and Dreissena polymorpha) from Lakes Garda and Maggiore, Northern Italy." Journal of Great Lakes Research 46, no. 1: 225-229.
Microplastics (MP) pollution has received increased attention over the last few years. However, while the number of studies documentating the ingestion of microplastics by fish has increased, fewer studies have addressed the toxicological effects derived from the ingestion of these small items in wild conditions. Here, MP contamination and effect biomarkers were investigated in three commercially important fish species from the North East Atlantic Ocean. From the 150 analysed fish (50 per species), 49 % had MP. In fish from the 3 species, MP in the gastrointestinal tract, gills and dorsal muscle were found. Fish with MP had significantly (p ≤ 0.05) higher lipid peroxidation levels in the brain, gills and dorsal muscle, and increased brain acetylcholinesterase activity than fish where no MP were found. These results suggest lipid oxidative damage in gills and muscle, and neurotoxicity through lipid oxidative damage and acetylcholinesterase induction in relation to MP and/or MP-associated chemicals exposure. From the 150 fish analysed, 32 % had MP in dorsal muscle, with a total mean (± SD) of 0.054 ± 0.099 MP items/g. Based on this mean and on EFSA recommendation for fish consumption by adults or the general population, human consumers of Dicentrachus labrax, Trachurus trachurus, Scomber colias may intake 842 MP items/year from fish consumption only. Based on the mean of MP in fish muscle and data (EUMOFA, NOAA) of fish consumption per capita in selected European and American countries, the estimated intake of microplastics through fish consumption ranged from 518 to 3078 MP items/year/capita. Considering that fish consumption is only one of the routes of human exposure to microplastics, this study and others in the literature emphasize the need for more research, risk assessment and adoption of measures to minimize human exposure to these particles. Thus, MP pollution and its effects should be further investigated and addressed according to the WHO ‘One Health’ approach.
Luís Gabriel A. Barboza; Clara Lopes; Patrícia Oliveira; Filipa Bessa; Vanessa Otero; Bruno Henriques; Joana Raimundo; Miguel Caetano; Carlos Vale; Lucia Guilhermino. Microplastics in wild fish from North East Atlantic Ocean and its potential for causing neurotoxic effects, lipid oxidative damage, and human health risks associated with ingestion exposure. Science of The Total Environment 2019, 717, 134625 .
AMA StyleLuís Gabriel A. Barboza, Clara Lopes, Patrícia Oliveira, Filipa Bessa, Vanessa Otero, Bruno Henriques, Joana Raimundo, Miguel Caetano, Carlos Vale, Lucia Guilhermino. Microplastics in wild fish from North East Atlantic Ocean and its potential for causing neurotoxic effects, lipid oxidative damage, and human health risks associated with ingestion exposure. Science of The Total Environment. 2019; 717 ():134625.
Chicago/Turabian StyleLuís Gabriel A. Barboza; Clara Lopes; Patrícia Oliveira; Filipa Bessa; Vanessa Otero; Bruno Henriques; Joana Raimundo; Miguel Caetano; Carlos Vale; Lucia Guilhermino. 2019. "Microplastics in wild fish from North East Atlantic Ocean and its potential for causing neurotoxic effects, lipid oxidative damage, and human health risks associated with ingestion exposure." Science of The Total Environment 717, no. : 134625.
Microplastics (MPs) were found to modulate the toxicity of other pollutants but the knowledge on the topic is still limited. The goals of this study were to investigate the short-term toxicity of cadmium (Cd) to wild Pomatochistus microps juveniles, the potential modulation of acute Cd toxicity by 1–5 µm polyethylene MPs in this species, and possible differences of sensitivity to Cd and MPs-Cd mixtures between juveniles from two distinct wild populations. Juveniles were collected in the estuaries of Minho (M-est) and Lima (L-est) Rivers (NW Portugal). One 96 h bioassay with M-est juveniles and another one with L-est juveniles were carried out in laboratory conditions. Each bioassay had 12 treatments: control, 5 Cd concentrations, 1 MPs concentration, and 5 MPs-Cd mixtures. No significant differences in Cd-induced mortality between juveniles from distinct estuaries or between juveniles exposed to Cd alone and those exposed to MPs-Cd mixtures were found. The total 96h LC10 and LC50 of Cd alone were 2 mg/L (95% CI: 0–4 mg/L) and 8 mg/L (95% CI: 2–17 mg/L), respectively. Cd alone significantly decreased the post-exposure predatory performance (PEPP) of M-est (≥6 mg/L) and L-est juveniles (≥3 mg/L), and acetylcholinesterase (AChE) activity of M-est juveniles (13 mg/L). MPs alone (0.14 mg/L) significantly reduced the PEPP and AChE activity of L-est juveniles but not of M-est juveniles. MPs-Cd mixtures (3–13 mg/L of Cd + 0.14 mg/L of MPs) significantly inhibited the PEPP of juveniles from both estuaries and AChE of L-est estuary juveniles but not of M-est juveniles. Evidences of toxicological interactions, namely antagonism, between MPs and Cd were found. Overall, the results indicate that MPs modulated the sub-lethal toxic effects of Cd in wild P. microps juveniles, especially neurotoxicity. Moreover, the environmental conditions of the natural habitats to which juveniles were exposed during pre-developmental phases influence the sub-lethal toxicity of Cd, MPs, and their mixtures. The implications to environmental and human risk assessment are discussed and further research is needed.
Tiago Miranda; Luis R. Vieira; Lúcia Guilhermino. Neurotoxicity, Behavior, and Lethal Effects of Cadmium, Microplastics, and Their Mixtures on Pomatoschistus microps Juveniles from Two Wild Populations Exposed under Laboratory Conditions―Implications to Environmental and Human Risk Assessment. International Journal of Environmental Research and Public Health 2019, 16, 2857 .
AMA StyleTiago Miranda, Luis R. Vieira, Lúcia Guilhermino. Neurotoxicity, Behavior, and Lethal Effects of Cadmium, Microplastics, and Their Mixtures on Pomatoschistus microps Juveniles from Two Wild Populations Exposed under Laboratory Conditions―Implications to Environmental and Human Risk Assessment. International Journal of Environmental Research and Public Health. 2019; 16 (16):2857.
Chicago/Turabian StyleTiago Miranda; Luis R. Vieira; Lúcia Guilhermino. 2019. "Neurotoxicity, Behavior, and Lethal Effects of Cadmium, Microplastics, and Their Mixtures on Pomatoschistus microps Juveniles from Two Wild Populations Exposed under Laboratory Conditions―Implications to Environmental and Human Risk Assessment." International Journal of Environmental Research and Public Health 16, no. 16: 2857.
The widespread use of microplastics and nanomaterials resulting in environmental contamination is of high concern. Microplastics have been found to modulate the toxicity of other environmental contaminants. Thus, the hypothesis that microplastics increase the toxicity of gold nanoparticles to the marine microalgae Tetraselmis chuii was tested. In a laboratory bioassay, T. chuii cultures were exposed for 96 h to ∼5 nm diameter gold nanoparticles (AuNP) and to virgin 1–5 μm diameter microplastics (MP), alone and in mixture. The treatments were: control; citrate-control; AuNP alone (0.1, 0.3 and 3 mg/L); MP alone (0.3, 0.9 and 4 mg/L) and mixture of the two substances in three different concentrations (0.1 mg/L AuNP + 0.3 mg/L MP; 0.3 mg/L AuNP + 0.9 mg/L MP; 3 mg/l AuNP + 4 mg/L MP). The effect criterion was the inhibition of the average specific growth rate. AuNP alone and MP alone did not cause significant decrease of T. chui average specific growth rate up to 3 mg/L and 4 mg/L, respectively. The mixture containing 3 mg/L AuNP + 4 mg/L MP significantly reduced the average specific growth rate of the microalgae. Therefore, this mixture was more toxic to T. chuii than its components individually. Overall, the results of the present study indicated that the MP and AuNP tested have a relatively low toxicity to T. chuii, but the toxicity increases when they are in mixtures containing high concentrations of both substances. These proof-of-concept findings stress the need of more research on the toxicity of mixtures containing microplastics and nanomaterials.
Elham Davarpanah; Lúcia Guilhermino. Are gold nanoparticles and microplastics mixtures more toxic to the marine microalgae Tetraselmis chuii than the substances individually? Ecotoxicology and Environmental Safety 2019, 181, 60 -68.
AMA StyleElham Davarpanah, Lúcia Guilhermino. Are gold nanoparticles and microplastics mixtures more toxic to the marine microalgae Tetraselmis chuii than the substances individually? Ecotoxicology and Environmental Safety. 2019; 181 ():60-68.
Chicago/Turabian StyleElham Davarpanah; Lúcia Guilhermino. 2019. "Are gold nanoparticles and microplastics mixtures more toxic to the marine microalgae Tetraselmis chuii than the substances individually?" Ecotoxicology and Environmental Safety 181, no. : 60-68.
Biomarkers use to be recorded in sentinel mussels for biological effects assessment in coastal pollution monitoring. There are recommended procedures (e.g., by ICES) for biomarker determination but not for sample collection and transport. However, where and when mussels are collected and how samples are processed can be a major source of variability in biomarker values. Thus, the present study aims at contributing to identify reliable practices for collection and transport of intertidal mussels in biomarker-based coastal pollution monitoring. Mussels, Mytilus galloprovincialis, were handpicked from the high (2.5–3.0 m) and low (0.5–1.0 m) tide-mark levels at autumn tides in Gorliz, a relatively clean locality of the Basque Coast (Bay of Biscay). Then, they were processed by different ways: (a) in situ dissected immediately after sampling; (b) transported to the laboratory either in air or in seawater; and (c) dissected at different time intervals after sampling. Oxidative stress, lysosomal and tissue-level biomarkers were investigated. Oxidative stress and lysosomal biomarkers were significantly affected by collection and transport to the laboratory, due to the combination of air exposure (hypoxia) and digestion process. Consequently, it is recommended that sentinel mussels should be collected the nearest possible to the 0 tide-mark level and processed in situ as soon as possible, and always within 1 h upon collection. If in situ processing is not possible, mussels should be transported in air and processed within the shortest time possible. Finally, details on sampling should be always provided to made possible the proper interpretation and comparison of results.
E. Blanco-Rayón; Lucia Guilhermino; I. Marigómez; U. Izagirre. Collection and transport of sentinel mussels in biomarker-based coastal pollution monitoring: Current flaws and reliable practices. Ecological Indicators 2019, 103, 722 -734.
AMA StyleE. Blanco-Rayón, Lucia Guilhermino, I. Marigómez, U. Izagirre. Collection and transport of sentinel mussels in biomarker-based coastal pollution monitoring: Current flaws and reliable practices. Ecological Indicators. 2019; 103 ():722-734.
Chicago/Turabian StyleE. Blanco-Rayón; Lucia Guilhermino; I. Marigómez; U. Izagirre. 2019. "Collection and transport of sentinel mussels in biomarker-based coastal pollution monitoring: Current flaws and reliable practices." Ecological Indicators 103, no. : 722-734.
Microplastics (MPs) are plastic particles with less than 5 mm in size that are considered global environmental pollutants. The MPs present in the environment result from the successive breakdown of larger plastic pieces or from the direct input of micro- and nano-sized particles used in various industries and products available to consumers. Such MPs have been found in several wild species and other natural resources, including some consumed as food by humans, with possible adverse effects on ecosystem and human health. The central aim of this work was to review the published literature regarding the contamination of sea commercial salts (sea and terrestrial origins) and its possible impacts on human health. Moreover, to lead to a comprehensive understanding of the paradigm, a short introduction and revision of the environmental contamination by MPs and its effects are included. MPs have been found in commercial salts from 128 brands, from 38 different countries spanning over five continents. The concentration of MPs found in the samples analysed is lower than the concentrations of MPs reported in other resources, such as blue mussels. However, as commercial salts are used every day and by all humans, they constitute a long-term exposure route for the general population in addition to others (e.g., animals consumed as food by humans, water, air). Therefore, commercial salts contaminated with MPs may contribute to the potential long-term adverse effects resulting from human exposure to these particles.
Diogo Peixoto; Carlos Pinheiro; João Amorim; Luis Oliva Teles; Lúcia Guilhermino; Natividade Vieira. Microplastic pollution in commercial salt for human consumption: A review. Estuarine, Coastal and Shelf Science 2019, 219, 161 -168.
AMA StyleDiogo Peixoto, Carlos Pinheiro, João Amorim, Luis Oliva Teles, Lúcia Guilhermino, Natividade Vieira. Microplastic pollution in commercial salt for human consumption: A review. Estuarine, Coastal and Shelf Science. 2019; 219 ():161-168.
Chicago/Turabian StyleDiogo Peixoto; Carlos Pinheiro; João Amorim; Luis Oliva Teles; Lúcia Guilhermino; Natividade Vieira. 2019. "Microplastic pollution in commercial salt for human consumption: A review." Estuarine, Coastal and Shelf Science 219, no. : 161-168.
Luís R. Vieira; Fernando Morgado; António J.A. Nogueira; Amadeu M.V.M. Soares; Lúcia Guilhermino. Integrated multivariate approach of ecological and ecotoxicological parameters in coastal environmental monitoring studies. Ecological Indicators 2018, 95, 1128 -1142.
AMA StyleLuís R. Vieira, Fernando Morgado, António J.A. Nogueira, Amadeu M.V.M. Soares, Lúcia Guilhermino. Integrated multivariate approach of ecological and ecotoxicological parameters in coastal environmental monitoring studies. Ecological Indicators. 2018; 95 ():1128-1142.
Chicago/Turabian StyleLuís R. Vieira; Fernando Morgado; António J.A. Nogueira; Amadeu M.V.M. Soares; Lúcia Guilhermino. 2018. "Integrated multivariate approach of ecological and ecotoxicological parameters in coastal environmental monitoring studies." Ecological Indicators 95, no. : 1128-1142.
Mussels are widely used in toxicological experimentation; however, experimental setups are not standardized yet. Although there is evidence of changes in biomarker values during food digestion and depending on the mussel nutritive status, the mode of feeding differs among toxicological experiments. Typically, mussels are fed with different diets in different long-term experiments, while fasting is the most common approach for short-term studies. Consequently, comparisons among experiments and reliable interpretations of biomarker results are often unfeasible. The present investigation aimed at determining the influence of fasting (against feeding with Isochrysis galbana) on biomarkers and their responsiveness in mussels exposed for 96 h to the water accommodated fraction (WAF) of a heavy fuel oil (0%, 6.25%, 12.5% and 25% WAF in sea water). PAH tissue levels in digestive gland and a battery of biomarkers were compared. WAF exposure led to decrease of cytochrome-C-oxidase activity, modulated glutathione-S-transferase activity, augmented lipid peroxidation, inhibited acetyl cholinesterase (AChE) activity, and led to lysosomal enlargement (VvLYS and S/VLYS) and membrane destabilisation, lipofuscin accumulation, and histopathological alterations (VvBAS, MLR/MET and CTD ratio) in the digestive gland epithelium; and were integrated as IBR/n (biological response index). Overall, no significant changes were recorded in AChE activity, S/VLYS and CTD ratio in any experimental treatment, while all the other biomarkers showed significant changes depending on the fasting/feeding condition, the exposure to WAF and/or their interaction. As a result, the integrated biomarker index IBR/n was higher at increasing WAF exposure levels both in fasted and fed mussels albeit the response was more marked in the latter. The response profiles were qualitatively similar between fasted and fed mussels but quantitatively more pronounced in fed mussels, especially upon exposure to the highest concentration (25% WAF). Therefore, it is highly recommended that mussels are also supplied with food during short-term, like during long-term toxicological experiments. This practice would avoid the interference of fasting with biological responses elicited by the tested chemicals and allow for reliable comparison with data obtained in long-term experiments and monitoring programmes.
E. Blanco-Rayón; L. Guilhermino; M. Irazola; A.V. Ivanina; I.M. Sokolova; Urtzi Izagirre; I. Marigómez. The influence of short-term experimental fasting on biomarker responsiveness in oil WAF exposed mussels. Aquatic Toxicology 2018, 206, 164 -175.
AMA StyleE. Blanco-Rayón, L. Guilhermino, M. Irazola, A.V. Ivanina, I.M. Sokolova, Urtzi Izagirre, I. Marigómez. The influence of short-term experimental fasting on biomarker responsiveness in oil WAF exposed mussels. Aquatic Toxicology. 2018; 206 ():164-175.
Chicago/Turabian StyleE. Blanco-Rayón; L. Guilhermino; M. Irazola; A.V. Ivanina; I.M. Sokolova; Urtzi Izagirre; I. Marigómez. 2018. "The influence of short-term experimental fasting on biomarker responsiveness in oil WAF exposed mussels." Aquatic Toxicology 206, no. : 164-175.
The presence of microplastics and several other pollutants in the marine environment is of growing concern. However, the knowledge on the toxicity of mixtures containing microplastics and other contaminants to marine species is still scarce. The main goals of this study were to investigate the oxidative stress and lipid oxidative damage potentially induced by 96 h of exposure to mercury (0.010 and 0.016 mg/L), microplastics (0.26 and 0.69 mg/L), and mixtures of the two substances (same concentrations, full factorial) in the gills and liver of D. labrax juveniles, and the possible influence of microplastics on mercury bioconcentration (gills) and bioaccumulation (liver). The results indicate that the presence of microplastics in the water increased the concentration of mercury in gills and liver of D. labrax juveniles. Microplastics and mercury, alone and in mixtures, caused oxidative stress in both organs. Based on the total induction of antioxidant enzymatic activity, the type of toxicological interaction in fish exposed to the mixture containing the lowest concentration of the two substances was addition in gills, and addition or synergism in the liver. These results stress the need to further address the role of microplastics in the bioconcentration, bioaccumulation, and toxicity of other environmental contaminants in different species.
Luís Gabriel Antão Barboza; Luis Vieira; Vasco Branco; Cristina Carvalho; Lucia Guilhermino. Microplastics increase mercury bioconcentration in gills and bioaccumulation in the liver, and cause oxidative stress and damage in Dicentrarchus labrax juveniles. Scientific Reports 2018, 8, 1 -9.
AMA StyleLuís Gabriel Antão Barboza, Luis Vieira, Vasco Branco, Cristina Carvalho, Lucia Guilhermino. Microplastics increase mercury bioconcentration in gills and bioaccumulation in the liver, and cause oxidative stress and damage in Dicentrarchus labrax juveniles. Scientific Reports. 2018; 8 (1):1-9.
Chicago/Turabian StyleLuís Gabriel Antão Barboza; Luis Vieira; Vasco Branco; Cristina Carvalho; Lucia Guilhermino. 2018. "Microplastics increase mercury bioconcentration in gills and bioaccumulation in the liver, and cause oxidative stress and damage in Dicentrarchus labrax juveniles." Scientific Reports 8, no. 1: 1-9.
The main objectives of this study were to investigate the effects of a mixture of microplastics and mercury on Corbicula fluminea, the post-exposure recovery, and the potential of microplastics to influence the bioconcentration of mercury by this species. Bivalves were collected in the field and acclimated to laboratory conditions for 14 days. Then, a 14-day bioassay was carried out. Bivalves were exposed for 8 days to clean medium (control), microplastics (0.13 mg/L), mercury (30 µg/L) and to a mixture (same concentrations) of both substances. The post-exposure recovery was investigated through 6 additional days in clean medium. After 8 and 14 days, the following endpoints were analysed: the post-exposure filtration rate (FR); the activity of cholinesterase enzymes (ChE), NADP-dependent isocitrate dehydrogenase (IDH), octopine dehydrogenase, catalase, glutathione reductase, glutathione peroxidase and glutathione S-transferases (GST), and the levels of lipid peroxidation (LPO). After 8 days of exposure to mercury, the bioconcentration factors (BCF) were 55 in bivalves exposed to the metal alone and 25 in bivalves exposed to the mixture. Thus, microplastics reduced the bioconcentration of mercury by C. fluminea. Bivalves exposed to microplastics, mercury or to the mixture had significantly (p ≤ 0.05) decreased FR and increased LPO levels, indicating fitness reduction and lipid oxidative damage. In addition, bivalves exposed to microplastics alone had significant (p ≤ 0.05) reduction of adductor muscle ChE activity, indicating neurotoxicity. Moreover, bivalves exposed to mercury alone had significantly (p ≤ 0.05) inhibited IDH activity, suggesting alterations in cellular energy production. Antagonism between microplastics and mercury in FR, ChE activity, GST activity and LPO levels was found. Six days of post-exposure recovery in clean medium was not enough to totally reverse the toxic effects induced by the substances nor to eliminate completely the mercury from the bivalve’s body. These findings have implications to animal, ecosystem and human health.
Patrícia Oliveira; Luis Gabriel Antão Barboza; Vasco Branco; Neusa Figueiredo; Cristina Carvalho; Lúcia Guilhermino. Effects of microplastics and mercury in the freshwater bivalve Corbicula fluminea (Müller, 1774): Filtration rate, biochemical biomarkers and mercury bioconcentration. Ecotoxicology and Environmental Safety 2018, 164, 155 -163.
AMA StylePatrícia Oliveira, Luis Gabriel Antão Barboza, Vasco Branco, Neusa Figueiredo, Cristina Carvalho, Lúcia Guilhermino. Effects of microplastics and mercury in the freshwater bivalve Corbicula fluminea (Müller, 1774): Filtration rate, biochemical biomarkers and mercury bioconcentration. Ecotoxicology and Environmental Safety. 2018; 164 ():155-163.
Chicago/Turabian StylePatrícia Oliveira; Luis Gabriel Antão Barboza; Vasco Branco; Neusa Figueiredo; Cristina Carvalho; Lúcia Guilhermino. 2018. "Effects of microplastics and mercury in the freshwater bivalve Corbicula fluminea (Müller, 1774): Filtration rate, biochemical biomarkers and mercury bioconcentration." Ecotoxicology and Environmental Safety 164, no. : 155-163.
The environmental contamination by microplastics is a global challenge to ecosystem and human health, and the knowledge on the long-term effects of such particles is limited. Thus, the effects of microplastics and post-exposure recovery were investigated over 4 generations (F, F, F, F) using Daphnia magna as model. Effect criteria were parental mortality, growth, several reproductive parameters, and population growth rate. Microplastics exposure (0.1mg/l of pristine polymer microspheres 1-5μm diameter) caused parental mortality (10-100%), and significantly (p≤0.05) decreased growth, reproduction, and population growth rate leading to the extinction of the microplastics-exposed model population in the F generation. Females descending from those exposed to microplastics in F and exposed to clean medium presented some recovery but up to the F generation they still had significantly (p≤0.05) reduced growth, reproduction, and population growth rate. Overall, these results indicate that D. magna recovery from chronic exposure to microplastics may take several generations, and that the continuous exposure over generations to microplastics may cause population extinction. These findings have implications to aquatic ecosystem functioning and services, and raise concern on the long-term animal and human exposure to microplastics through diverse routes.
Alexandra Martins; Lúcia Guilhermino. Transgenerational effects and recovery of microplastics exposure in model populations of the freshwater cladoceran Daphnia magna Straus. Science of The Total Environment 2018, 631-632, 421 -428.
AMA StyleAlexandra Martins, Lúcia Guilhermino. Transgenerational effects and recovery of microplastics exposure in model populations of the freshwater cladoceran Daphnia magna Straus. Science of The Total Environment. 2018; 631-632 ():421-428.
Chicago/Turabian StyleAlexandra Martins; Lúcia Guilhermino. 2018. "Transgenerational effects and recovery of microplastics exposure in model populations of the freshwater cladoceran Daphnia magna Straus." Science of The Total Environment 631-632, no. : 421-428.
Recent studies have demonstrated the negative impacts of microplastics on wildlife. Therefore, the presence of microplastics in marine species for human consumption and the high intake of seafood (fish and shellfish) in some countries cause concern about the potential effects of microplastics on human health. In this brief review, the evidence of seafood contamination by microplastics is reviewed, and the potential consequences of the presence of microplastics in the marine environment for human food security, food safety and health are discussed. Furthermore, challenges and gaps in knowledge are identified. The knowledge on the adverse effects on human health due to the consumption of marine organisms containing microplastics is very limited, difficult to assess and still controversial. Thus, assessment of the risk posed to humans is challenging. Research is urgently needed, especially regarding the potential exposure and associated health risk to micro- and nano-sized plastics.
Luís Gabriel Antão Barboza; A. Dick Vethaak; Beatriz R.B.O. Lavorante; Anne-Katrine Lundebye; Lúcia Guilhermino. Marine microplastic debris: An emerging issue for food security, food safety and human health. Marine Pollution Bulletin 2018, 133, 336 -348.
AMA StyleLuís Gabriel Antão Barboza, A. Dick Vethaak, Beatriz R.B.O. Lavorante, Anne-Katrine Lundebye, Lúcia Guilhermino. Marine microplastic debris: An emerging issue for food security, food safety and human health. Marine Pollution Bulletin. 2018; 133 ():336-348.
Chicago/Turabian StyleLuís Gabriel Antão Barboza; A. Dick Vethaak; Beatriz R.B.O. Lavorante; Anne-Katrine Lundebye; Lúcia Guilhermino. 2018. "Marine microplastic debris: An emerging issue for food security, food safety and human health." Marine Pollution Bulletin 133, no. : 336-348.
The effects of emerging environmental contaminants on human and environmental health is of high concern, especially those potentially induced by mixtures. The main goal of the present study was to assess the chronic effects of mixtures of citrate stabilized ≈5 nm gold nanoparticles (AuNP) and 1-5μm microplastics (MP) on Daphnia magna. A 21-day bioassay was carried out. The effect criteria were parental mortality, somatic growth and several reproductive parameters. AuNP induced parental mortality, reduced the total offspring and caused immobile juveniles and aborted eggs. MP induced parental mortality, delayed the first brood release, decreased the number of broods released, the total offspring, and caused immobile juveniles. All the mixtures caused higher toxicity than AuNP and MP alone. Based on parental mortality, evidences of antagonism between AuNP and MP were observed at low concentrations of both mixture components, whereas evidences of synergism at high concentrations were found. Chronic (21-day) exposure of D. magna to AuNPs, MP, and their mixtures can impair development, reproduction, ultimately leading to death.
Alexandre Pacheco; Alexandra Martins; Lúcia Guilhermino. Toxicological interactions induced by chronic exposure to gold nanoparticles and microplastics mixtures in Daphnia magna. Science of The Total Environment 2018, 628-629, 474 -483.
AMA StyleAlexandre Pacheco, Alexandra Martins, Lúcia Guilhermino. Toxicological interactions induced by chronic exposure to gold nanoparticles and microplastics mixtures in Daphnia magna. Science of The Total Environment. 2018; 628-629 ():474-483.
Chicago/Turabian StyleAlexandre Pacheco; Alexandra Martins; Lúcia Guilhermino. 2018. "Toxicological interactions induced by chronic exposure to gold nanoparticles and microplastics mixtures in Daphnia magna." Science of The Total Environment 628-629, no. : 474-483.
Microplastics and mercury are environmental pollutants of great concern. The main goal of the present study was to investigate the effects of these pollutants, both individually and in binary mixtures, on the swimming performance of juvenile European seabass, Dicentrarchus labrax. Microplastics alone, mercury alone and all the mixtures caused significant reduction of the swimming velocity and resistance time of fish. Moreover, changes in behavioural responses including lethargic and erratic swimming behaviour were observed. These results highlight that fish behavioural responses can be used as sensitive endpoint to establish the effects of contamination by microplastics and also emphasizes the need to assess the combined effects of microplastics and other environmental contaminants, with special attention to the effects on behavioural responses in fish and other aquatic species.
Luís Gabriel Antão Barboza; Luís Russo Vieira; Lúcia Guilhermino. Single and combined effects of microplastics and mercury on juveniles of the European seabass (Dicentrarchus labrax): Changes in behavioural responses and reduction of swimming velocity and resistance time. Environmental Pollution 2018, 236, 1014 -1019.
AMA StyleLuís Gabriel Antão Barboza, Luís Russo Vieira, Lúcia Guilhermino. Single and combined effects of microplastics and mercury on juveniles of the European seabass (Dicentrarchus labrax): Changes in behavioural responses and reduction of swimming velocity and resistance time. Environmental Pollution. 2018; 236 ():1014-1019.
Chicago/Turabian StyleLuís Gabriel Antão Barboza; Luís Russo Vieira; Lúcia Guilhermino. 2018. "Single and combined effects of microplastics and mercury on juveniles of the European seabass (Dicentrarchus labrax): Changes in behavioural responses and reduction of swimming velocity and resistance time." Environmental Pollution 236, no. : 1014-1019.