One of the problems of optical remote sensing of crop above-ground biomass (AGB) is that vegetation indices (VIs) often saturate from the middle to late growth stages. This study focuses on combining VIs acquired by a consumer-grade multiple-spectral UAV and machine learning regression techniques to (i) determine the optimal time window for AGB estimation of winter wheat and to (ii) determine the optimal combination of multi-spectral VIs and regression algorithms. UAV-based multi-spectral data and manually measured AGB of winter wheat, under five nitrogen rates, were obtained from the jointing stage until 25 days after flowering in the growing season 2020/2021. Forty-four multi-spectral VIs were used in the linear regression (LR), partial least squares regression (PLSR), and random forest (RF) models in this study. Results of LR models showed that the heading stage was the most suitable stage for AGB prediction, with R² values varying from 0.48 to 0.93. Three PLSR models based on different datasets performed differently in estimating AGB in the training dataset (R² = 0.74~0.92, RMSE = 0.95~2.87 t/ha, MAE = 0.75~2.18 t/ha, and RPD = 2.00~3.67) and validation dataset (R² = 0.50~0.75, RMSE = 1.56~2.57 t/ha, MAE = 1.44~2.05 t/ha, RPD = 1.45~1.89). Compared with PLSR models, the performance of the RF models was more stable in the prediction of AGB in the training dataset (R² = 0.95~0.97, RMSE = 0.58~1.08 t/ha, MAE = 0.46~0.89 t/ha, and RPD = 3.95~6.35) and validation dataset (R² = 0.83~0.93, RMSE = 0.93~2.34 t/ha, MAE = 0.72~2.01 t/ha, RPD = 1.36~3.79). Monitoring AGB prior to flowering was found to be more effective than post-flowering. Moreover, this study demonstrates that it is feasible to estimate AGB for multiple growth stages of winter wheat by combining the optimal VIs and PLSR and RF models, which overcomes the saturation problem of using individual VI-based linear regression models.

Spectral indices rarely show consistency in estimating crop traits across growth stages; thus, it is critical to simultaneously evaluate a group of spectral variables and select the most informative spectral indices for retrieving crop traits. The objective of this study was to explore the optimal spectral predictors for above-ground biomass (AGB) by applying Random Forest (RF) on three types of spectral predictors: the full spectrum, published spectral indices (Pub-SIs), and optimized spectral indices (Opt-SIs). Canopy hyperspectral reflectance of potato plants, treated with seven nitrogen (N) rates, was obtained during the tuber formation and tuber bulking from 2015 to 2016. Twelve Pub-SIs were selected, and their spectral bands were optimized using band optimization algorithms. Results showed that the Opt-SIs were the best input variables of RF models. Compared to the best empirical model based on Opt-SIs, the Opt-SIs based RF model improved the prediction of AGB, with R² increased by 6%, 10%, and 16% at the tuber formation, tuber bulking, and for across the two growth stages, respectively. The Opt-SIs can significantly reduce the number of input variables. The optimized Blue nitrogen index (Opt-BNI) and Modified red-edge normalized difference vegetation index (Opt-mND705) combined with an RF model showed the best performance in estimating potato AGB at the tuber formation stage (R² = 0.88). In the tuber bulking stage, only using optimized Nitrogen planar domain index (Opt-NPDI) as the input variable of the RF model produced satisfactory accuracy in training and testing datasets, with the R², RMSE, and RE being 0.92, 208.6 kg/ha, and 10.3%, respectively. The Opt-BNI and Double-peak nitrogen index (Opt-NDDA) coupling with an RF model explained 86% of the variations in potato AGB, with the lowest RMSE (262.9 kg/ha) and RE (14.8%) across two growth stages. This study shows that combining the Opt-SIs and RF can greatly enhance the prediction accuracy for crop AGB while significantly reduces collinearity and redundancies of spectral data.

Green vegetation is an essential part of natural resources and is vital to the ecosystem. Simultaneously, with improving people’s living standards, food security and the supply of forage resources have become increasingly the focus of attention. Therefore, timely and accurate monitoring and accurate and timely vegetation classification are significant for the rational utilization of agricultural resources. In recent years, the unmanned aerial vehicle (UAV) platform has attracted considerable attention and achieved great success in the application of remote sensing identification of vegetation due to the combination of the advantages of satellite and airborne systems. However, the results of many studies haven’t yet been synthesized to provide practical guidance for improving recognition performance. This study aimed to introduce the primary classifiers used for UAV remote-sensing vegetation identification and conducted a meta-analysis of relevant research on UAV remote-sensing vegetation identification. This meta-analysis reviewed 79 papers, analyzed the general characteristics of spatial and temporal distribution and journal sources, and compared the relationship between research objectives, sensor types, spatial resolution, research methods, number of target categories, and the overall accuracy of the results. Finally, a detailed review was conducted on how unmanned aerial vehicle remote sensing is applied in vegetation identification, along with the current unresolved issues and prospects.

Unmanned Aerial Systems (UASs) are increasingly vital in precision agriculture, offering detailed, real-time insights into plant health across multiple spectral domains. However, this technology’s precision in estimating plant traits associated with Nitrogen Use Efficiency (NUE), and the factors affecting this precision, are not well-documented. This review examines the capabilities of UASs in assessing NUE in crops. Our analysis specifically highlights how different growth stages critically influence NUE and biomass assessments in crops and reveals a significant impact of specific signal processing techniques and sensor types on the accuracy of remote sensing data. Optimized flight parameters and precise sensor calibration are underscored as key for ensuring the reliability and validity of collected data. Additionally, the review delves into how different canopy structures, like planophile and erect leaf orientations, uniquely influence spectral data interpretation. The study also recognizes the untapped potential of image texture features in UAV-based remote sensing for detailed analysis of canopy micro-architecture. Overall, this research not only underscores the transformative impact of UAS technology on agricultural productivity and sustainability but also demonstrates its potential in providing more accurate and comprehensive insights for effective crop health and nutrient management strategies.

Excessive nitrogen (N) fertilization poses environmental risks at regional and global levels. Satellite remote sensing provides a novel approach for large-scale N monitoring. In this study, we evaluated the performance of different types of spectral bands and indices (SIs) coupled with ensemble learning models (ELMs) at retrieving the plant N concentration (PNC) and plant aboveground biomass (AGB) of potato from Sentinel-2 images. Cloud-free Sentinel-2 imagery was acquired during the tuber-formation to starch-accumulation stages from 2020 to 2021. Fourteen optimal SIs were selected using the successive projections algorithm (SPA) and principal component analysis (PCA). The PNC and AGB estimation models were then built using an ELMs. The results showed that the SIs based on chlorophyll absorption bands were strongly related to potato PNC and AGB. Also, the N-correlated bands were mainly concentrated in the red-edge (705 nm) and short-wave infrared (1610 and 2190 nm) regions. The ELMs successfully predicted PNC and AGB (R²_PNC = 0.74; R²_AGB = 0.82). Compared with the other five base models (k-nearest neighbor (KNN), partial least squares regression (PLSR), support vector regression (SVR), random forest (RF), and Gaussian process regression (GPR)), the ELMs provided higher PNC and AGB estimation accuracy and effectively reduced overfitting to training data. This study demonstrated that the promising solution of using SPA-PCA coupled with an ensemble learning model improves the estimation accuracy of potato PNC and AGB based on Sentinel-2 imagery data.