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Gymnodimine A has been found in mollusks obtained along the whole northern coast of Spain from April 2017 to December 2019. This is the first time that this toxin is detected in mollusks from the Atlantic coast of Europe. The prevalence of the toxin was, in general, low, being detected on average in approximately 6% of the obtained samples (122 out of 1900). The concentrations recorded were also, in general, low, with a median of 1.3 μg kg−1, and a maximum value of 23.93 μg kg−1. The maxima of prevalence and concentration were not geographically coincident, taking place the first at the easternmost part of the sampled area and the second at the westernmost part. In most cases (>94%), gymnodimine A and 13-desmethyl spirolide C were concurrently detected, suggesting that Alexandrium ostenfeldii could be the responsible producer species. The existence of cases in which gymnodimine A was detected alone suggests also that a Karenia species could also be involved. The geographical heterogeneity of the distribution suggests that blooms of the producer species are mostly local. Not all bivalves are equally affected, clams being less affected than mussels, oysters, and razor clams. Due to their relatively low toxicity, and their low prevalence and concentration, it seems that these toxins do not pose an important risk for the mollusk consumers in the area.
J.Pablo Lamas; Fabiola Arévalo; Ángeles Moroño; Jorge Correa; Araceli E. Rossignoli; Juan Blanco. Gymnodimine A in mollusks from the north Atlantic Coast of Spain: Prevalence, concentration, and relationship with spirolides. Environmental Pollution 2021, 279, 116919 .
AMA StyleJ.Pablo Lamas, Fabiola Arévalo, Ángeles Moroño, Jorge Correa, Araceli E. Rossignoli, Juan Blanco. Gymnodimine A in mollusks from the north Atlantic Coast of Spain: Prevalence, concentration, and relationship with spirolides. Environmental Pollution. 2021; 279 ():116919.
Chicago/Turabian StyleJ.Pablo Lamas; Fabiola Arévalo; Ángeles Moroño; Jorge Correa; Araceli E. Rossignoli; Juan Blanco. 2021. "Gymnodimine A in mollusks from the north Atlantic Coast of Spain: Prevalence, concentration, and relationship with spirolides." Environmental Pollution 279, no. : 116919.
For the first time, pinnatoxins have been detected in shellfish from the Atlantic and Cantabrian coasts of Spain. High sensitivity LC-MS/MS systems were used to monitor all the currently known pinnatoxins (A–H). Pinnatoxin G (PnTX G) was the most prevalent toxin of the group, but its metabolite PnTX A has also been found at much lower levels. No trend in PnTX G concentration was found in the area, but a hotspot in the Ría de Camariñas has been identified. The maximum concentrations found did not exceed 15 µg·kg−1, being, in most cases, below 3 µg·kg−1. The highest concentrations were found in wild (intertidal) populations of mussels which attained much higher levels than raft-cultured ones, suggesting that the toxin-producer organisms preferentially develop in shallow areas. Other bivalve species had, in general, lower concentrations. The incidence of PnTX G followed a seasonal pattern in which the maximum concentrations took place in winter months. PnTX G was found to be partially esterified but the esterification percentage was not high (lower than 30%).
J. Pablo Lamas; Fabiola Arévalo; Ángeles Moroño; Jorge Correa; Susana Muñíz; Juan Blanco. Detection and Spatio-Temporal Distribution of Pinnatoxins in Shellfish from the Atlantic and Cantabrian Coasts of Spain. Toxins 2019, 11, 340 .
AMA StyleJ. Pablo Lamas, Fabiola Arévalo, Ángeles Moroño, Jorge Correa, Susana Muñíz, Juan Blanco. Detection and Spatio-Temporal Distribution of Pinnatoxins in Shellfish from the Atlantic and Cantabrian Coasts of Spain. Toxins. 2019; 11 (6):340.
Chicago/Turabian StyleJ. Pablo Lamas; Fabiola Arévalo; Ángeles Moroño; Jorge Correa; Susana Muñíz; Juan Blanco. 2019. "Detection and Spatio-Temporal Distribution of Pinnatoxins in Shellfish from the Atlantic and Cantabrian Coasts of Spain." Toxins 11, no. 6: 340.
A method based on micro-matrix solid-phase dispersion (μ-MSPD) followed by gas-chromatography tandem mass spectrometry (GC–MS/MS) was developed to analyze UV filters in personal care products. It is the first time that MSPD is employed to extract UV filters from cosmetics samples. This technique provides efficient and low-cost extractions, and allows performing extraction and clean-up in one step, which is one of their main advantages. The amount of sample employed was only 0.1 g and the extraction procedure was performed preparing the sample-sorbent column in a glass Pasteur pipette instead of the classic plastic columns in order to avoid plastizicer contamination. Factors affecting the process such as type of sorbent, and amount and type of elution solvent were studied by a factorial design. The method was validated and extended to other families of cosmetic ingredients such as fragrance allergens, preservatives, plasticizers and synthetic musks, including a total of 78 target analytes. Recovery studies in real sample at several concentration levels were also performed. Finally, the green extraction methodology was applied to the analysis of real cosmetic samples of different nature.
Maria Celeiro; Lua Vazquez; J. Pablo Lamas; Marlene Vila; Carmen Garcia-Jares; Maria Llompart. Miniaturized Matrix Solid-Phase Dispersion for the Analysis of Ultraviolet Filters and Other Cosmetic Ingredients in Personal Care Products. Separations 2019, 6, 30 .
AMA StyleMaria Celeiro, Lua Vazquez, J. Pablo Lamas, Marlene Vila, Carmen Garcia-Jares, Maria Llompart. Miniaturized Matrix Solid-Phase Dispersion for the Analysis of Ultraviolet Filters and Other Cosmetic Ingredients in Personal Care Products. Separations. 2019; 6 (2):30.
Chicago/Turabian StyleMaria Celeiro; Lua Vazquez; J. Pablo Lamas; Marlene Vila; Carmen Garcia-Jares; Maria Llompart. 2019. "Miniaturized Matrix Solid-Phase Dispersion for the Analysis of Ultraviolet Filters and Other Cosmetic Ingredients in Personal Care Products." Separations 6, no. 2: 30.
Os subprodutos de elaboración do viño conteñen valiosos compostos bioactivos, entre os que destacan os polifenois. Os efectos beneficiosos destes compostos atribúense, entre outras, á súa actividade antioxidante. Por esta razón, estúdase o efecto potencial dunha dieta enriquecida en polifenois nas características da carne de tenreiros alimentados con bagazo de uva como complemento ou suplemento na súa alimentación. Proponse unha extracción rápida de seis polifenois individuais de carne crúa de tenreiro, previamente identificados e seleccionados como marcadores no bagazo. O procedemento baséase na extracción simultánea automatizada mediante líquidos presurizados (PLE) seguida de cromatografía líquida de alta eficacia con detección UV-Vis cun sistema de díodos (HPLC-DAD) para a identificación dos polifenois e a análise cuantitativo. Nos extractos obtidos determínanse o índice de polifenois totais (IPT) e a actividade antioxidante (AA). Debido á complexidade da mostra, os resultados máis sobresaíntes deste traballo son os relacionados coa avaliación da actividade antioxidante dos extractos. Tras a correspondente análise estatística do conxunto de datos obtidos, pode observarse que resulta mellor incluír unha combinación de bagazo na dieta dos animais dada a mellora observada nos índices antioxidantes medidos, e en consecuencia, tamén nas propiedades da carne e na súa conservación.
Laura Rubio; Juan Pablo Lamas; Carmen García-Jares; Marta Lores. Actividade Antioxidante en Carne de Tenreiros Alimentados con Bagazo de Uva. Recursos Rurais 2019, 1 .
AMA StyleLaura Rubio, Juan Pablo Lamas, Carmen García-Jares, Marta Lores. Actividade Antioxidante en Carne de Tenreiros Alimentados con Bagazo de Uva. Recursos Rurais. 2019; (14):1.
Chicago/Turabian StyleLaura Rubio; Juan Pablo Lamas; Carmen García-Jares; Marta Lores. 2019. "Actividade Antioxidante en Carne de Tenreiros Alimentados con Bagazo de Uva." Recursos Rurais , no. 14: 1.
In this work, matrix solid-phase dispersion (MSPD) is proposed for the first time as an efficient and rapid alternative to extract fatty acids (FAs) from grape seeds. In addition, the use of green solvents as limonene allowed obtaining similar yields to other longer extraction procedures using more unhealthy solvents. The optimization of the MSPD procedure was performed using experimental design strategies and considering the use of solvents such as ethyl lactate and limonene with interesting possibilities for the extraction of bioactive compounds from natural sources. Gas chromatography-mass spectrometry (GC-MS) was used to analyze the seed extracts. The MSPD-GC-MS method was applied to characterize the FA profile of 27 seed samples from grapes belonging to Vitis vinifera white cultivars (Albariño, Caiño, Godello, Loureiro, Torrontés, Treixadura, Gewurtzträminer, Pinot blanc, and Pinot gris); a red cultivar (Mencía); a teinturier cultivar (Alicante Bouschet); and two hybrids producing white and rose grapes, respectively. Results showed that unsaturated FAs represented 82% of the total, with linoleic being the main acid (73.7%). The content of saturated FAs was 12.5%. Despite slight differences between cultivars, quite similar profiles were obtained independently of the cultivar, the type of sample (fresh grapes or distillation wastes), and the collection year, which is a very interesting conclusion for a potential exploitation of seeds as a source of good-quality oil. A simple technique such as MSPD using limonene as extraction solvent constituted an efficient and greener alternative for the extraction of grape seed FAs.
Laura Rubio; Juan Pablo Lamas; Marta Lores; Carmen Garcia-Jares. Matrix Solid-Phase Dispersion Using Limonene as Greener Alternative for Grape Seeds Extraction, Followed by GC-MS Analysis for Varietal Fatty Acid Profiling. Food Analytical Methods 2018, 11, 3235 -3242.
AMA StyleLaura Rubio, Juan Pablo Lamas, Marta Lores, Carmen Garcia-Jares. Matrix Solid-Phase Dispersion Using Limonene as Greener Alternative for Grape Seeds Extraction, Followed by GC-MS Analysis for Varietal Fatty Acid Profiling. Food Analytical Methods. 2018; 11 (11):3235-3242.
Chicago/Turabian StyleLaura Rubio; Juan Pablo Lamas; Marta Lores; Carmen Garcia-Jares. 2018. "Matrix Solid-Phase Dispersion Using Limonene as Greener Alternative for Grape Seeds Extraction, Followed by GC-MS Analysis for Varietal Fatty Acid Profiling." Food Analytical Methods 11, no. 11: 3235-3242.
Influence of sucrose and trehalose addition on epimerisation and thermal degradation of catechin and epicatechin have been studied. Sucrose addition accelerated epimerisation of catechin and epicatechin at 80 and 100 °C. Addition of trehalose had protective effect on epimerisation of catechin and epicatechin. Considering thermal degradation, it was confirmed that thermal degradation of catechin and epicatechin follows the first-order kinetics. Increasing the temperature causes shorter half-lives of catechin and epicatechin. Addition of sucrose had negative effect on catechin stability. However, in samples with epicatechin, sucrose impact was temperature dependent. Trehalose addition had positive impact on preventing catechin and epicatechin degradation almost doubling the half-life of studied compounds. Data presented in this study could help to predict chemical conversions of investigated phenolics in various food systems and to prolong their shelf life in thermally processed foods.
Ante Lončarić; Juan Pablo Lamas; Eugenia Guerra; Mirela Kopjar; Marta Lores. Thermal stability of catechin and epicatechin upon disaccharides addition. International Journal of Food Science & Technology 2017, 53, 1195 -1202.
AMA StyleAnte Lončarić, Juan Pablo Lamas, Eugenia Guerra, Mirela Kopjar, Marta Lores. Thermal stability of catechin and epicatechin upon disaccharides addition. International Journal of Food Science & Technology. 2017; 53 (5):1195-1202.
Chicago/Turabian StyleAnte Lončarić; Juan Pablo Lamas; Eugenia Guerra; Mirela Kopjar; Marta Lores. 2017. "Thermal stability of catechin and epicatechin upon disaccharides addition." International Journal of Food Science & Technology 53, no. 5: 1195-1202.
Camellia (genus of flowering plants of fam. Theaceae) is one of the main crops in Asia, where tea and oil from leaves and seeds have been utilized for thousands of years. This plant is excellently adapted to the climate and soil of Galicia (northwestern Spain) and northern Portugal where it is grown not only as an ornamental plant, but to be evaluated as a source of bioactive compounds. In this work, the main fatty acids were extracted from Camellia seeds of four varieties of Camellia: sasanqua, reticulata, japonica and sinensis, by means of matrix-solid phase dispersion (MSPD), and analyzed by gas chromatography (GC) with MS detection of the corresponding methyl esters. MSPD constitutes an efficient and greener alternative to conventional extraction techniques, moreover if it is combined with the use of green solvents such as limonene. The optimization of the MSPD extraction procedure has been conducted using a multivariate approach based on strategies of experimental design, which enabled the simultaneous evaluation of the factors influencing the extraction efficiency as well as interactions between factors. The optimized method was applied to characterize the fatty acids profiles of four Camellia varieties seeds, allowing us to compare their fatty acid composition.
Carmen Garcia-Jares; Marta Sanchez-Nande; Juan Pablo Lamas; Marta Lores. Profiling the Fatty Acids Content of Ornamental Camellia Seeds Cultivated in Galicia by an Optimized Matrix Solid-Phase Dispersion Extraction. Bioengineering 2017, 4, 87 .
AMA StyleCarmen Garcia-Jares, Marta Sanchez-Nande, Juan Pablo Lamas, Marta Lores. Profiling the Fatty Acids Content of Ornamental Camellia Seeds Cultivated in Galicia by an Optimized Matrix Solid-Phase Dispersion Extraction. Bioengineering. 2017; 4 (4):87.
Chicago/Turabian StyleCarmen Garcia-Jares; Marta Sanchez-Nande; Juan Pablo Lamas; Marta Lores. 2017. "Profiling the Fatty Acids Content of Ornamental Camellia Seeds Cultivated in Galicia by an Optimized Matrix Solid-Phase Dispersion Extraction." Bioengineering 4, no. 4: 87.
The use of a matrix solid-phase dispersion (MSPD) process to extract polyphenols from hawthorn (Crataegus oxyacantha L.) a deciduous shrub with an expected rich phytochemical profile, has been evaluated. MSPD extracts of fruits and leaves have an outstanding content of polyphenols, although the particular phenolic profile is solvent dependent. The extracts were analysed by HPLC-DAD for the accurate identification of the major bioactive polyphenols, some of which have never been described for this species. MSPD has proven to be a good alternative to the classic methods of obtaining natural extracts, fast and with low consumption of organic solvents, therefore, environmentally friendly. The bioactivities can be considered also very remarkable, revealing extracts with high levels of antioxidant activity.
Wassila Benabderrahmane; Marta Lores; Juan Pablo Lamas; Samir Benayache. Matrix solid-phase dispersion as a tool for phytochemical and bioactivities characterisation: Crataegus oxyacantha L._A case study. Natural Product Research 2017, 32, 1220 -1223.
AMA StyleWassila Benabderrahmane, Marta Lores, Juan Pablo Lamas, Samir Benayache. Matrix solid-phase dispersion as a tool for phytochemical and bioactivities characterisation: Crataegus oxyacantha L._A case study. Natural Product Research. 2017; 32 (10):1220-1223.
Chicago/Turabian StyleWassila Benabderrahmane; Marta Lores; Juan Pablo Lamas; Samir Benayache. 2017. "Matrix solid-phase dispersion as a tool for phytochemical and bioactivities characterisation: Crataegus oxyacantha L._A case study." Natural Product Research 32, no. 10: 1220-1223.
Grape seeds represent a high percentage (20% to 26%) of the grape marc obtained as a byproduct from white winemaking and keep a vast proportion of grape polyphenols. In this study, seeds obtained from 11 monovarietal white grape marcs cultivated in Northwestern Spain have been analyzed in order to characterize their polyphenolic content and antioxidant activity. Seeds of native (Albariño, Caiño, Godello, Loureiro, Torrontés, and Treixadura) and non-native (Chardonnay, Gewurtzträminer, Pinot blanc, Pinot gris, and Riesling) grape varieties have been considered. Low weight phenolics have been extracted by means of pressurized liquid extraction (PLE) and further analyzed by LC-MS/MS. The results showed that PLE extracts, whatever the grape variety of origin, contained large amounts of polyphenols and high antioxidant activity. Differences in the varietal polyphenolic profiles were found, so a selective exploitation of seeds might be possible.
Carmen Garcia-Jares; Alberto Vazquez; Juan P. Lamas; Marta Pajaro; Marta Alvarez-Casas; Marta Lores. Antioxidant White Grape Seed Phenolics: Pressurized Liquid Extracts from Different Varieties. Antioxidants 2015, 4, 737 -749.
AMA StyleCarmen Garcia-Jares, Alberto Vazquez, Juan P. Lamas, Marta Pajaro, Marta Alvarez-Casas, Marta Lores. Antioxidant White Grape Seed Phenolics: Pressurized Liquid Extracts from Different Varieties. Antioxidants. 2015; 4 (4):737-749.
Chicago/Turabian StyleCarmen Garcia-Jares; Alberto Vazquez; Juan P. Lamas; Marta Pajaro; Marta Alvarez-Casas; Marta Lores. 2015. "Antioxidant White Grape Seed Phenolics: Pressurized Liquid Extracts from Different Varieties." Antioxidants 4, no. 4: 737-749.
Fragrance allergens, preservatives, plasticizers, and synthetic musks are usually present in cosmetic and personal care products formulations and many of them are subjected to use restrictions or labeling requirements. Matrix solid-phase dispersion (MSPD) is a very suitable analytical technique for the extraction of these compounds providing a simple, low cost sample preparation, and the possibility of performing both extraction and clean-up in one step, reducing possible contamination and analyte losses. This extraction technique has been successfully applied to many cosmetics ingredients allowing obtaining quantitative recoveries. A new very simple micro-MSPD procedure performing the disruption step in a vial is proposed for the gas chromatography-mass spectrometry (GC-MS) analysis of 66 chemicals usually present in cosmetics and personal care products. The method was validated showing general recoveries between 80% and 110%, relative standard deviation (RSD) values lower than 15%, and limits of detection (LODs) below 30 ng·g−1. The validated method was applied to a broad range of cosmetics and personal care products, including several products intended for baby care.
Maria Celeiro; Juan Pablo Lamas; Maria Llompart; Carmen Garcia-Jares. In-Vial Micro-Matrix-Solid Phase Dispersion for the Analysis of Fragrance Allergens, Preservatives, Plasticizers, and Musks in Cosmetics. Cosmetics 2014, 1, 171 -201.
AMA StyleMaria Celeiro, Juan Pablo Lamas, Maria Llompart, Carmen Garcia-Jares. In-Vial Micro-Matrix-Solid Phase Dispersion for the Analysis of Fragrance Allergens, Preservatives, Plasticizers, and Musks in Cosmetics. Cosmetics. 2014; 1 (3):171-201.
Chicago/Turabian StyleMaria Celeiro; Juan Pablo Lamas; Maria Llompart; Carmen Garcia-Jares. 2014. "In-Vial Micro-Matrix-Solid Phase Dispersion for the Analysis of Fragrance Allergens, Preservatives, Plasticizers, and Musks in Cosmetics." Cosmetics 1, no. 3: 171-201.
Fragrance suspected allergens including those regulated by the EU Directive 76/768/EEC have been determined in different types of waters using solid-phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS). The procedure was based on headspace sampling (HS-SPME) using polydimethylsiloxane/divinylbenzene (PDMS/DVB) fibers and has been optimized by an experimental design approach. The method performance has been studied showing good linearity (R ≥ 0.994) as well as good intra-day and inter-day precision (RSD ≤ 12%). Detection limits (S/N = 3) ranged from 0.001 to 0.3 ng mL−1. Reliability was demonstrated through the quantitative recoveries of the compounds in real water samples, including baby bathwaters, swimming pool waters, and wastewaters. The absence of matrix effects allowed quantification of the compounds by external aqueous calibration. The analysis of 35 samples of different types of waters showed the presence of suspected allergens in all the analyzed samples. All targets were found in the samples, with the exception of methyl eugenol and amyl cinnamic alcohol. Highest concentrations of suspected allergens were present in baby bathwaters, containing from 5 to 15 of the compounds at concentrations ranging from few pg mL−1 to several hundreds of ng mL−1.
Elias Becerril; J. Pablo Lamas; Lucia Sanchez-Prado; Maria Llompart; Marta Lores; Carmen Garcia-Jares. Analysis of regulated suspected allergens in waters. Talanta 2010, 83, 464 -474.
AMA StyleElias Becerril, J. Pablo Lamas, Lucia Sanchez-Prado, Maria Llompart, Marta Lores, Carmen Garcia-Jares. Analysis of regulated suspected allergens in waters. Talanta. 2010; 83 (2):464-474.
Chicago/Turabian StyleElias Becerril; J. Pablo Lamas; Lucia Sanchez-Prado; Maria Llompart; Marta Lores; Carmen Garcia-Jares. 2010. "Analysis of regulated suspected allergens in waters." Talanta 83, no. 2: 464-474.