In the last decade or so, polychlorinated biphenyls (PCBs) garnered renewed attention in the scientific community due to new evidence pointing at their continued presence in the environment and workplaces and the potential human risks related to their presence. PCBs move from the environment to humans through different routes; the dominant pathway is the ingestion of contaminated foods (fish, seafood and dairy products), followed by inhalation (both indoor and outdoor air), and, to a lesser extent, dust ingestion and dermal contact. Numerous studies reported the environmental and occupational exposure to these pollutants, deriving from building materials (flame-retardants, plasticizers, paints, caulking compounds, sealants, fluorescent light ballasts, etc.) and electrical equipment. The highest PCBs contaminations were detected in e-waste recycling sites, suggesting the need for the implementation of remediation strategies of such polluted areas to safeguard the health of workers and local populations. Furthermore, a significant correlation between PCB exposure and increased blood PCB concentrations was observed in people working in PCB-contaminated workplaces. Several epidemiological studies suggest that environmental and occupational exposure to high concentrations of PCBs is associated with different health outcomes, such as neuropsychological and neurobehavioral deficits, dementia, immune system dysfunctions, cardiovascular diseases and cancer. In addition, recent studies indicate that PCBs bioaccumulation can reduce fertility, with harmful effects on the reproductive system that can be passed to offspring. In the near future, further studies are needed to assess the real effects of PCBs exposure at low concentrations for prolonged exposure in workplaces and specific indoor environments.

Polycyclic aromatic hydrocarbons (PAHs) are a group of organic compounds with fused aromatic rings, primarily derived from combustion processes and environmental pollutants. This narrative review discusses the most relevant studies on PAHs, focusing on their sources, environmental and occupational exposure, and effects on human health, emphasizing their roles as carcinogenic, mutagenic, and teratogenic agents. The primary pathways for human exposure to PAHs are through the ingestion of contaminated food (mainly due to some food processing methods, such as smoking and high-temperature cooking techniques), the inhalation of ambient air, and the smoking of cigarettes. Coke oven workers are recognized as a high-risk occupational group for PAH exposure, highlighting the need for appropriate strategies to mitigate these risks and safeguard worker health. PAHs are metabolized into reactive intermediates in the body, which can lead to DNA damage and promote the development of various health conditions, particularly in environments with high exposure levels. Chronic PAH exposure has been linked to respiratory diseases, as well as cardiovascular problems and immune system suppression. Furthermore, this review underscores the significant impact of PAHs on reproductive health. The results of the reported studies suggest that both male and female fertility can be compromised due to oxidative stress, DNA damage, and endocrine disruption caused by PAH exposure. In males, PAHs impair sperm quality, while, in females, they disrupt ovarian function, potentially leading to infertility, miscarriage, and birth defects. Fetal exposure to PAHs is also associated with neurodevelopmental disorders. Given the extensive and detrimental health risks posed by PAHs, this review stresses the importance of stringent environmental regulations, occupational safety measures, and public health initiatives to mitigate exposure and safeguard reproductive and overall health.

Several studies have assessed the presence of microplastics (MPs) in human biological fluids and tissues highlighting potential health risks associated to oxidative stress, inflammation, immune dysfunction, neurotoxicity and reprotoxicity. However, only few studies have evaluated MP presence and effects in ovarian tissues of mammalians and, to date, no studies have detected MPs in human ovarian follicular fluids. Based on these premises, in this study, 18 women (undergoing assisted reproductive treatment at In Vitro Fertilisation center in Salerno, Southern Italy) were selected to assess the presence of MPs in follicular fluid. Plastic particles < 10 µm were measured using Scanning Electron Microscopy (SEM) coupled with an EDX (X Energy Dispersion) detector. MPs (size <10 µm) were detected in 14 out of 18 samples of follicular fluid, with an average concentration of 2191 particles/mL (0-7181particles/mL) and with a mean diameter of 4.48 µm (3.18-5.54 µm). Moreover, a significant correlation between MP concentration in follicular fluid samples and Follicle-Stimulating Hormone (FSH) (p-value <0.05), as well as a weak (non-significant) correlation with Body Mass Index (BMI), age and 17β-estradiol (E2), was found. On the contrary, no correlation with anti-Müllerian Hormone (AMH), fertilization outcomes, miscarriages, or live birth was observed. Since several studies on animal models have demonstrated the negative effects of MPs on ovarian function, the present study, that verified for the first time the presence of MPs in human follicular fluid, is of great significance for the scientific community in terms of raising awareness of the impact that these increasingly pervasive emerging contaminants have on reproductive function and human health.

Purpose: The SARS‐CoV‐2 pandemic prompted the development and use of next‐generation vaccines. Among these, mRNA‐based vaccines consist of injectable solutions of mRNA encoding for a recombinant Spike, which is distinguishable from the wild‐type protein due to specific amino acid variations introduced to maintain the protein in a prefused state. This work presents a proteomic approach to reveal the presence of recombinant Spike protein in vaccinated subjects regardless of antibody titer.Experimental design: Mass spectrometry examination of biological samples was used to detect the presence of specific fragments of recombinant Spike protein in subjects who received mRNA‐based vaccines.Results: The specific PP‐Spike fragment was found in 50% of the biological samples analyzed, and its presence was independent of the SARS‐CoV‐2 IgG antibody titer. The minimum and maximum time at which PP‐Spike was detected after vaccination was 69 and 187 days, respectively.Conclusions and clinical relevance: The presented method allows to evaluate the half‐life of the Spike protein molecule “PP” and to consider the risks or benefits in continuing to administer additional booster doses of the SARS‐CoV‐2 mRNA vaccine. This approach is of valuable support to complement antibody level monitoring and represents the first proteomic detection of recombinant Spike in vaccinated subjects.

DNA oxidative damage is one of the main concerns being implicated in severe cell alterations, promoting different types of human disorders and diseases. For their characteristics, male gametes are the most sensitive cells to the accumulation of damaged DNA. We have recently reported the relevance of arginine residues in the Cu(II)-induced DNA breakage of sperm H1 histones. In this work, we have extended our previous findings investigating the involvement of human sperm nuclear basic proteins on DNA oxidative damage in healthy males presenting copper and chromium excess in their semen. We found in 84% of those males an altered protamines/histones ratio and a different DNA binding mode even for those presenting a canonical protamines/histones ratio. Furthermore, all the sperm nuclear basic proteins from these samples that resulted were involved in DNA oxidative damage, supporting the idea that these proteins could promote the Fenton reaction in DNA proximity by increasing the availability of these metals near the binding surface of DNA. In conclusion, our study reveals a new and unexpected behavior of human sperm nuclear basic proteins in oxidative DNA damage, providing new insights for understanding the mechanisms related to processes in which oxidative DNA damage is implicated.

The aim of this study was to evaluate changes in the chemical profile of essential oils and antioxidant enzymes activity (catalase CAT, superoxide dismutase SOD, Glutathione S-transferases GST, and Peroxidase POX) in Mentha × piperita L. (Mitcham variety) and Mentha arvensis L. (var. piperascens), in response to heat stress. In addition, we used salicylic acid (SA) and melatonin (M), two brassinosteroids that play an important role in regulating physiological processes, to assess their potential to mitigate heat stress. In both species, the heat stress caused a variation in the composition of the essential oils and in the antioxidant enzymatic activity. Furthermore both Salicylic acid (SA) and melatonin (M) alleviated the effect of heat stress.