To investigate the cellular effects of terahertz (THz) exposure, human corneal epithelial (HCE-T) cells derived from human eye were exposed to 0.12 THz radiation at 5 mW/cm² for 24 h, then the genotoxicity, morphological changes, and heat shock protein (Hsp) expression of the cells were examined. There was no statistically significant increase in the micronucleus (MN) frequency of cells exposed to 0.12 THz radiation compared with sham-exposed controls and incubator controls, whereas the MN frequency of cells treated with bleomycin for 1 h (positive control) did increase significantly. Similarly, there were no significant morphological changes in cells exposed to 0.12 THz radiation compared to sham-exposed controls and incubator controls, and Hsp expression (Hsp27, Hsp70, and Hsp90α) was also not significantly different between the three treatments. These results indicate that exposure to 0.12 THz radiation using the present conditions appears to have no or very little effect on MN formation, morphological changes, and Hsp expression in cells derived from human eye.

Human corneal epithelial (HCE-T) and human lens epithelial (SRA01/04) cells derived from the human eye were exposed to 60 gigahertz (GHz) millimeter-wavelength radiation for 24 h. There was no statistically significant increase in the micronucleus (MN) frequency in cells exposed to 60 GHz millimeter-wavelength radiation at 1 mW/cm² compared with sham-exposed controls and incubator controls. The MN frequency of cells treated with bleomycin for 1 h provided positive controls. The comet assay, used to detect DNA strand breaks, and heat shock protein (Hsp) expression also showed no statistically significant effects of exposure. These results indicate that exposure to millimeter-wavelength radiation has no effect on genotoxicity in human eye cells.

Recent progress in wireless technologies has made human exposure to electromagnetic fields (EMFs) increasingly complex. The situation can increase public concerns related to possible health effects due to EMF exposure. Monitoring EMF exposure levels and characterizing them are indispensable for risk communications of human exposure to EMFs. From this background, a project on the acquisition, accumulation, and applications of EMF exposure monitoring data in Japan was started in 2019. One of the objectives of this project is to obtain a comprehensive picture of EMF exposure in actual daily lives. In 2019 and 2020, we measured the electric field (E-field) strength from mainly mobile phone base stations in the same areas as those in measurements conducted in 2006 and 2007 by the Ministry of Internal Affairs and Communications (MIC), Japan, and compared the data to investigate the time-course of the EMF environment. The number of measured points was 100 (10 × 10 grids) in an area of 1 km × 1 km in two urban and two suburban areas, and that in an underground shopping mall was 158. This large-scale study is the first in Japan. As a result, we found that the measured E-field strengths tended to be higher in 2019 and 2020 than those in 2006 and 2007, especially in the mall. However, the median ratios to the Japanese radio wave protection guideline values for urban areas and malls are lower than −40 dB.

Car-mounted measurements of radiofrequency electromagnetic exposure levels were carried out in a large area around Tokyo. Prior to the electric field (E-field) measurements using a car, the effect of the car body was evaluated in an anechoic chamber. The measurements between May 2021 and February 2022 were carried out within a radius of 100 km centering on Nihonbashi, Tokyo, with a measurement distance of about 13,800 km. The measurement results were averaged in the reference area mesh (1 km²). It was found that the E-field strengths of FM/TV frequency bands are lower than that of mobile phone base stations. It was also found that the E-field strength of only the 5G frequency band is approximately 20–30 dB lower than that of all mobile phone systems. However, note that it is possible to depend on the data traffic of 5G. The E-field strength of all bands is higher in Tokyo than in other prefectures. Additionally, repeated measurements were carried out to investigate the reproducibility of the measured E-field. The standard deviation is less than 3 dB along the same route, and a similar tendency of E-field strength by the car to the time-averaged results of spot measurements in the past was confirmed. Finally, the relationship of E-field strength with population density was investigated. It was found that the E-field strength from mobile phone base stations has a positive relationship with population density.