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Dr. Vincenzo Landi
Università di Bari "Aldo Moro"

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0 Animal Breeding
0 Animal Science
0 Gene Expression
0 Genomics
0 Molecular Genetics

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Journal article
Published: 19 July 2021 in Forensic Sciences Research
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The probative value of animal forensic genetic evidence relies on laboratory accuracy and reliability. Inter-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their output. The International Society for Animal Genetics (ISAG) administered animal forensic comparison tests (AFCTs) in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification, parentage and species determination services. The AFCTs revealed that analyses of low DNA template concentrations (≤300 pg/µL) constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage results. Moreover, a lack of familiarity with species testing protocols, interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination results. Several laboratories showed improvement in their genotyping accuracy over time. However, the use of forensically validated standards, such as a standard forensic STR kit, preferably with an allelic ladder, and stricter guidelines for STR typing, may have prevented some common issues from occurring, such as genotyping inaccuracies, missing data, elevated stutter products and loading errors. The AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each other. Laboratories should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number (LCN) analysis and species testing. Although this is the first time that the ISAG has conducted comparison tests for forensic testing, findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing. Supplemental data for this article are available online at https://doi.org/10.1080/20961790.2021.1886679

ACS Style

Sreetharan Kanthaswamy; Torsten Brendel; Luis Cancela; Denise A. Andrade de Oliveira; Bertram Brenig; Carmen Cons; Julian A. Crespi; Markéta Dajbychová; Andreas Feldl; Tomohito Itoh; Vincenzo Landi; Amparo Martinez; Malgorzata Natonek-Wisniewska; Robert F. Oldt; Anna Radko; Oscar Ramírez; Clementina Rodellar; Manuel Ruiz-Girón; David Schikorski; María Elena Turba; Guillermo Giovambatista. An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics. Forensic Sciences Research 2021, 1 -14.

AMA Style

Sreetharan Kanthaswamy, Torsten Brendel, Luis Cancela, Denise A. Andrade de Oliveira, Bertram Brenig, Carmen Cons, Julian A. Crespi, Markéta Dajbychová, Andreas Feldl, Tomohito Itoh, Vincenzo Landi, Amparo Martinez, Malgorzata Natonek-Wisniewska, Robert F. Oldt, Anna Radko, Oscar Ramírez, Clementina Rodellar, Manuel Ruiz-Girón, David Schikorski, María Elena Turba, Guillermo Giovambatista. An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics. Forensic Sciences Research. 2021; ():1-14.

Chicago/Turabian Style

Sreetharan Kanthaswamy; Torsten Brendel; Luis Cancela; Denise A. Andrade de Oliveira; Bertram Brenig; Carmen Cons; Julian A. Crespi; Markéta Dajbychová; Andreas Feldl; Tomohito Itoh; Vincenzo Landi; Amparo Martinez; Malgorzata Natonek-Wisniewska; Robert F. Oldt; Anna Radko; Oscar Ramírez; Clementina Rodellar; Manuel Ruiz-Girón; David Schikorski; María Elena Turba; Guillermo Giovambatista. 2021. "An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics." Forensic Sciences Research , no. : 1-14.

Journal article
Published: 25 May 2021 in Genes
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Susceptibility to diseases is inherited and can be transmitted between populations. Single-nucleotide polymorphism (SNPs) in genes related to immune response is associated with diseases in cattle. This study investigated SNPs in the genomic region of cytokines in 702 samples of Curraleiro Pé-Duro cattle and associated them with the occurrence of antibodies in brucellosis, leptospirosis, neosporosis, leukosis, infectious bovine rhinotracheitis (IBR), and bovine viral diarrhea (BVD) tests. DNA samples were evaluated by the kompetitive allele-specific polymerase chain reaction (KASP) method to identify polymorphisms. The gametic phase and SNP haplotypes were determined with the help of PHASE 2.1.1 software. Haplotypes were associated with serological results against Brucella abortus, Leptospira sp., Neospora caninum, leukosis, infectious rhinotracheitis, and BVD using univariate analysis followed by logistic regression. Haplotype 2 of TLR2 was present in 70% of the animals that tested positive for N. caninum infection. Haplotypes of TLR10 and TLR6 and IL10RA were more common in seronegative animals. Haplotypes related to the gene IL10RA were associated with animals negative to all infections. Curraleiro Pé-Duro cattle presented polymorphisms related to resistance to bacterial, viral, and N. caninum infections.

ACS Style

Thais Freitas; Juliana Dias; Luanna Guimarães; Sáudio Peixoto; Rayanne Silva; Kareem Badr; Maria Moura; Adriana Carmo; Vincenzo Landi; Maria Fioravanti. Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle. Genes 2021, 12, 806 .

AMA Style

Thais Freitas, Juliana Dias, Luanna Guimarães, Sáudio Peixoto, Rayanne Silva, Kareem Badr, Maria Moura, Adriana Carmo, Vincenzo Landi, Maria Fioravanti. Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle. Genes. 2021; 12 (6):806.

Chicago/Turabian Style

Thais Freitas; Juliana Dias; Luanna Guimarães; Sáudio Peixoto; Rayanne Silva; Kareem Badr; Maria Moura; Adriana Carmo; Vincenzo Landi; Maria Fioravanti. 2021. "Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle." Genes 12, no. 6: 806.

Journal article
Published: 08 April 2021 in Animals
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Goats have important social and economic roles in many countries because of their ability to survive and be productive in marginal areas. The overarching aim of this study was to compare the application of Wood’s model to different test-day milk recording protocols for estimation of total milk, fat, and protein yield in dairy goats. A total of 465 goats were used (Garganica, 78; Girgentana, 81; Jonica, 76; Maltese, 77; Red Mediterranean, 76; Saanen, 77). Milk yield was recorded every 15 days throughout lactation of 210 days, for a total of 14 collection days, during both morning and afternoon milking sessions. Milk samples were collected and analyzed for protein and fat. The fat-corrected milk was standardized at 35g fat/kg of milk. Wood models showed high R 2 values, and thus good fitting, in all the considered breeds. Wood model applied to first, second, fourth, and sixth month recordings (C) and ICAR estimation showed total milk yield very close to Wood’s model applied to all 14 recordings (A) (p > 0.38). Differently, Wood’s model applied to the first, second, third, and fourth month recording (B) estimation showed great differences (p < 0.01). This could be applied for farms that had the necessity to synchronize flock groups for kidding in order to produce kid meat. In farms that apply the estrus induction and/or synchronization for kidding, it would be possible to perform only four test-day milk recordings and to apply the Wood’s model on them in order to obtain the estimation of total milk, fat, and protein yield during lactation for animals inscribed, or to be inscribed, to the genealogical book.

ACS Style

Vincenzo Landi; Aristide Maggiolino; Angela Salzano; Salvatore Claps; Pasquale De Palo; Domenico Rufrano; Giuseppina Pedota; Gianluca Neglia. Evaluation of Different Test-Day Milk Recording Protocols by Wood’s Model Application for the Estimation of Dairy Goat Milk and Milk Constituent Yield. Animals 2021, 11, 1058 .

AMA Style

Vincenzo Landi, Aristide Maggiolino, Angela Salzano, Salvatore Claps, Pasquale De Palo, Domenico Rufrano, Giuseppina Pedota, Gianluca Neglia. Evaluation of Different Test-Day Milk Recording Protocols by Wood’s Model Application for the Estimation of Dairy Goat Milk and Milk Constituent Yield. Animals. 2021; 11 (4):1058.

Chicago/Turabian Style

Vincenzo Landi; Aristide Maggiolino; Angela Salzano; Salvatore Claps; Pasquale De Palo; Domenico Rufrano; Giuseppina Pedota; Gianluca Neglia. 2021. "Evaluation of Different Test-Day Milk Recording Protocols by Wood’s Model Application for the Estimation of Dairy Goat Milk and Milk Constituent Yield." Animals 11, no. 4: 1058.

Journal article
Published: 01 December 2020 in Journal of Dairy Science
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Morphological traits are of great importance to dairy goat production given their effect on phenotypes of economic interest. However, their underlying genomic architecture has not yet been extensively characterized. Herein, we aimed to identify genomic regions associated with body, udder, and leg conformation traits recorded in 825 Murciano-Granadina goats. We genotyped this resource population using the GoatSNP50 BeadChip (Illumina Inc., San Diego, CA) and performed genome-wide association analyses using the GEMMA software. We found 2 genome-wide significant associations between markers rs268273468 [Capra hircus (CHI) 16:69617700] and rs268249346 (CHI 28:18321523) and medial suspensory ligament. In contrast, we did not detect any genome-wide significant associations for body and leg traits. Moreover, we found 12, 19, and 7 chromosome-wide significant associations for udder, body, and leg traits, respectively. Comparison of our data with previous studies revealed a low level of positional concordance between regions associated with morphological traits. In addition to technical factors, this lack of concordance could be due to a substantial level of genetic heterogeneity among breeds or to the strong polygenic background of morphological traits, which makes it difficult to detect genetic factors that have small phenotypic effects.

ACS Style

Maria Gracia Luigi-Sierra; Vincenzo Landi; Dailu Guan; Juan Vicente Delgado; Anna Castelló; Betlem Cabrera; Emilio Mármol-Sánchez; Javier Fernández Alvarez; Mayra Gómez-Carpio; Amparo Martínez; Xavier Such; Jordi Jordana; Marcel Amills. A genome-wide association analysis for body, udder, and leg conformation traits recorded in Murciano-Granadina goats. Journal of Dairy Science 2020, 103, 11605 -11617.

AMA Style

Maria Gracia Luigi-Sierra, Vincenzo Landi, Dailu Guan, Juan Vicente Delgado, Anna Castelló, Betlem Cabrera, Emilio Mármol-Sánchez, Javier Fernández Alvarez, Mayra Gómez-Carpio, Amparo Martínez, Xavier Such, Jordi Jordana, Marcel Amills. A genome-wide association analysis for body, udder, and leg conformation traits recorded in Murciano-Granadina goats. Journal of Dairy Science. 2020; 103 (12):11605-11617.

Chicago/Turabian Style

Maria Gracia Luigi-Sierra; Vincenzo Landi; Dailu Guan; Juan Vicente Delgado; Anna Castelló; Betlem Cabrera; Emilio Mármol-Sánchez; Javier Fernández Alvarez; Mayra Gómez-Carpio; Amparo Martínez; Xavier Such; Jordi Jordana; Marcel Amills. 2020. "A genome-wide association analysis for body, udder, and leg conformation traits recorded in Murciano-Granadina goats." Journal of Dairy Science 103, no. 12: 11605-11617.

Journal article
Published: 10 October 2020 in Animals
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Considering casein haplotype variants rather than SNPs may maximize the understanding of heritable mechanisms and their implication on the expression of functional traits related to milk production. Effects of casein complex haplotypes on milk yield, milk composition, and curve shape parameters were used using a Bayesian inference for ANOVA. We identified 48 single nucleotide polymorphisms (SNPs) present in the casein complex of 159 unrelated individuals of diverse ancestry, which were organized into 86 haplotypes. The Ali and Schaeffer model was chosen as the best fitting model for milk yield (Kg), protein, fat, dry matter, and lactose (%), while parabolic yield-density was chosen as the best fitting model for somatic cells count (SCC × 103 sc/mL). Peak and persistence for all traits were computed respectively. Statistically significant differences (p < 0.05) were found for milk yield and components. However, no significant difference was found for any curve shape parameter except for protein percentage peak. Those haplotypes for which higher milk yields were reported were the ones that had higher percentages for protein, fat, dry matter, and lactose, while the opposite trend was described by somatic cells counts. Conclusively, casein complex haplotypes can be considered in selection strategies for economically important traits in dairy goats.

ACS Style

María Gabriela Pizarro Inostroza; Francisco Javier Navas González; Vincenzo Landi; Jose Manuel León Jurado; Juan Vicente Delgado Bermejo; Javier Fernández Álvarez; María Del Amparo Martínez Martínez. Bayesian Analysis of the Association between Casein Complex Haplotype Variants and Milk Yield, Composition, and Curve Shape Parameters in Murciano-Granadina Goats. Animals 2020, 10, 1845 .

AMA Style

María Gabriela Pizarro Inostroza, Francisco Javier Navas González, Vincenzo Landi, Jose Manuel León Jurado, Juan Vicente Delgado Bermejo, Javier Fernández Álvarez, María Del Amparo Martínez Martínez. Bayesian Analysis of the Association between Casein Complex Haplotype Variants and Milk Yield, Composition, and Curve Shape Parameters in Murciano-Granadina Goats. Animals. 2020; 10 (10):1845.

Chicago/Turabian Style

María Gabriela Pizarro Inostroza; Francisco Javier Navas González; Vincenzo Landi; Jose Manuel León Jurado; Juan Vicente Delgado Bermejo; Javier Fernández Álvarez; María Del Amparo Martínez Martínez. 2020. "Bayesian Analysis of the Association between Casein Complex Haplotype Variants and Milk Yield, Composition, and Curve Shape Parameters in Murciano-Granadina Goats." Animals 10, no. 10: 1845.

Journal article
Published: 18 September 2020 in Animals
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SPSS syntax was described to evaluate the individual performance of 49 linear and non-linear models to fit the milk component evolution curve of 159 Murciano-Granadina does selected for genotyping analyses. Peak and persistence for protein, fat, dry matter, lactose, and somatic cell counts were evaluated using 3107 controls (3.91 ± 2.01 average lactations/goat). Best-fit (adjusted R2) values (0.548, 0.374, 0.429, and 0.624 for protein, fat, dry matter, and lactose content, respectively) were reached by the five-parameter logarithmic model of Ali and Schaeffer (ALISCH), and for the three-parameter model of parabolic yield-density (PARYLDENS) for somatic cell counts (0.481). Cross-validation was performed using the Minimum Mean-Square Error (MMSE). Model comparison was performed using Residual Sum of Squares (RSS), Mean-Squared Prediction Error (MSPE), adjusted R2 and its standard deviation (SD), Akaike (AIC), corrected Akaike (AICc), and Bayesian information criteria (BIC). The adjusted R2 SD across individuals was around 0.2 for all models. Thirty-nine models successfully fitted the individual lactation curve for all components. Parametric and computational complexity promote variability-capturing properties, while model flexibility does not significantly (p > 0.05) improve the predictive and explanatory potential. Conclusively, ALISCH and PARYLDENS can be used to study goat milk composition genetic variability as trustable evaluation models to face future challenges of the goat dairy industry.

ACS Style

María Gabriela Pizarro Inostroza; Francisco Javier Navas González; Vincenzo Landi; Jose Manuel León Jurado; Juan Vicente Delgado Bermejo; Javier Fernández Álvarez; María Del Amparo Martínez Martínez. Goat Milk Nutritional Quality Software-Automatized Individual Curve Model Fitting, Shape Parameters Calculation and Bayesian Flexibility Criteria Comparison. Animals 2020, 10, 1693 .

AMA Style

María Gabriela Pizarro Inostroza, Francisco Javier Navas González, Vincenzo Landi, Jose Manuel León Jurado, Juan Vicente Delgado Bermejo, Javier Fernández Álvarez, María Del Amparo Martínez Martínez. Goat Milk Nutritional Quality Software-Automatized Individual Curve Model Fitting, Shape Parameters Calculation and Bayesian Flexibility Criteria Comparison. Animals. 2020; 10 (9):1693.

Chicago/Turabian Style

María Gabriela Pizarro Inostroza; Francisco Javier Navas González; Vincenzo Landi; Jose Manuel León Jurado; Juan Vicente Delgado Bermejo; Javier Fernández Álvarez; María Del Amparo Martínez Martínez. 2020. "Goat Milk Nutritional Quality Software-Automatized Individual Curve Model Fitting, Shape Parameters Calculation and Bayesian Flexibility Criteria Comparison." Animals 10, no. 9: 1693.

Journal article
Published: 08 September 2020 in Animals
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The genetic origins and diversity of Creole sheep from five regions of Colombia were investigated based on mitochondrial DNA (mtDNA) variations across 89 sequences from five breeds: one wool Creole sheep (CL) and four hair Creole sheep, including Ethiopian (OPCE), Sudan (OPCS), Pelibuey (OPCP) and Wayúu (OPCW). A global comparison was done using 62 haplotypes from Iberian, African, Indian, Caribbean, Mexican, Caucasian and European sheep based on sequences retrieved from GenBank. This study aimed to identify the maternal origin of Colombian Creole sheep and their genetic relationships at a global level. The results showed 31 different haplotypes from Colombian Creole sheep, which can be assigned to maternal lineage B, the most common lineage found in European sheep breeds and the only one found in several Iberian breed (e.g., Churra, Spanish Merino) that most likely participated in the Creole formation. Additional analyses showed that wool and hair sheep retained a broad genetic identity despite being geographically separated. The global-level phylogenetic analysis revealed that Colombian Creole sheep belong to a distinct and defined genetic lineage that is likely the result of a founder effect with ecotypes of Iberian descent and the subsequent introduction of foreign breeds. This is consistent with historical reports on the presence of sheep in South America and, particularly, Colombia.

ACS Style

Herman Alberto Revelo; Diana López-Alvarez; Vincenzo Landi; Lauden Rizzo; Luz Angela Alvarez. Mitochondrial DNA Variations in Colombian Creole Sheep Confirm an Iberian Origin and Shed Light on the Dynamics of Introduction Events of African Genotypes. Animals 2020, 10, 1594 .

AMA Style

Herman Alberto Revelo, Diana López-Alvarez, Vincenzo Landi, Lauden Rizzo, Luz Angela Alvarez. Mitochondrial DNA Variations in Colombian Creole Sheep Confirm an Iberian Origin and Shed Light on the Dynamics of Introduction Events of African Genotypes. Animals. 2020; 10 (9):1594.

Chicago/Turabian Style

Herman Alberto Revelo; Diana López-Alvarez; Vincenzo Landi; Lauden Rizzo; Luz Angela Alvarez. 2020. "Mitochondrial DNA Variations in Colombian Creole Sheep Confirm an Iberian Origin and Shed Light on the Dynamics of Introduction Events of African Genotypes." Animals 10, no. 9: 1594.

Journal article
Published: 04 September 2020 in Mathematics
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SPSS model syntax was defined and used to evaluate the individual performance of 49 linear and non-linear models to fit the lactation curve of 159 Murciano-Granadina does selected for genotyping analyses. Lactation curve shape, peak and persistence were evaluated for each model using 3107 milk yield controls with an average of 3.78 ± 2.05 lactations per goat. Best fit (Adjusted R2) values (0.47) were reached by the five-parameter logarithmic model of Ali and Schaeffer. Three main possibilities were detected: non-fitting (did not converge), standard (Adjusted R2 over 75%) and atypical curves (Adjusted R2 below 75%). All the goats fitted for 38 models. The ability to fit different possible functional forms for each goat, which progressively increased with the number of parameters comprised in each model, translated into a higher sensitivity to explaining curve shape individual variability. However, for models for which all goats fitted, only moderate increases in explanatory and predictive potential (AIC, AICc or BIC) were found. The Ali and Schaeffer model reported the best fitting results to study the genetic variability behind goat milk yield and perhaps enhance the evaluation of curve parameters as trustable future selection criteria to face the future challenges offered by the goat dairy industry.

ACS Style

María Gabriela Pizarro Inostroza; Francisco Javier Navas González; Vincenzo Landi; José Manuel León Jurado; Juan Vicente Delgado Bermejo; Javier Fernández Álvarez; María Del Amparo Martínez Martínez. Software-Automatized Individual Lactation Model Fitting, Peak and Persistence and Bayesian Criteria Comparison for Milk Yield Genetic Studies in Murciano-Granadina Goats. Mathematics 2020, 8, 1505 .

AMA Style

María Gabriela Pizarro Inostroza, Francisco Javier Navas González, Vincenzo Landi, José Manuel León Jurado, Juan Vicente Delgado Bermejo, Javier Fernández Álvarez, María Del Amparo Martínez Martínez. Software-Automatized Individual Lactation Model Fitting, Peak and Persistence and Bayesian Criteria Comparison for Milk Yield Genetic Studies in Murciano-Granadina Goats. Mathematics. 2020; 8 (9):1505.

Chicago/Turabian Style

María Gabriela Pizarro Inostroza; Francisco Javier Navas González; Vincenzo Landi; José Manuel León Jurado; Juan Vicente Delgado Bermejo; Javier Fernández Álvarez; María Del Amparo Martínez Martínez. 2020. "Software-Automatized Individual Lactation Model Fitting, Peak and Persistence and Bayesian Criteria Comparison for Milk Yield Genetic Studies in Murciano-Granadina Goats." Mathematics 8, no. 9: 1505.

Journal article
Published: 13 August 2020 in Italian Journal of Animal Science
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ACS Style

Víctor Fernando Torres-Aburto; Manuel Barrientos-Morales; Herminio Hernández-Flores; Araceli Rodríguez-Andrade; Patricia Cervantes-Acosta; Vincenzo Landi; Antonio Hernández Beltran; Belisario Domínguez-Mancera. Breeding soundness examination and herd proficiency of local genetic groups of bulls in tropical environment conditions in Veracruz, Mexico. Italian Journal of Animal Science 2020, 19, 840 -855.

AMA Style

Víctor Fernando Torres-Aburto, Manuel Barrientos-Morales, Herminio Hernández-Flores, Araceli Rodríguez-Andrade, Patricia Cervantes-Acosta, Vincenzo Landi, Antonio Hernández Beltran, Belisario Domínguez-Mancera. Breeding soundness examination and herd proficiency of local genetic groups of bulls in tropical environment conditions in Veracruz, Mexico. Italian Journal of Animal Science. 2020; 19 (1):840-855.

Chicago/Turabian Style

Víctor Fernando Torres-Aburto; Manuel Barrientos-Morales; Herminio Hernández-Flores; Araceli Rodríguez-Andrade; Patricia Cervantes-Acosta; Vincenzo Landi; Antonio Hernández Beltran; Belisario Domínguez-Mancera. 2020. "Breeding soundness examination and herd proficiency of local genetic groups of bulls in tropical environment conditions in Veracruz, Mexico." Italian Journal of Animal Science 19, no. 1: 840-855.

Journal article
Published: 11 August 2020 in Revista MVZ Córdoba
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Objetivo. Caracterizar genéticamente los ovinos criollos colombianos y sus relaciones con razas de origen europeo. Materiales y métodos. 261 muestras de sangre de las siguientes poblaciones, fueron colectadas: Criollos de Lana (CL), Mora Colombiana (MC), Criollo de Pelo (CP), mestizos (Mes), Hampshire (Hamp), Corriedale (Corr), Katahdin (Kath), Pelibuey (Pel), En 40 fincas de ocho departamentos (Córdoba, Magdalena, Cesar, Atlántico, Valle del Cauca, Nariño, Boyacá y Tolima) y 30 muestras de Merino Español (ME), Merino Precoz (MP), Merinofleischschaf (MF), Segureño (Seg) y Uda (UD) de Nigeria. Un total de 15 marcadores microsatélites fueron incluidos en este estudio. Resultados. En ovinos criollos, el número promedio de alelos encontrado fue 6.20±1.48 (CL), 7.27±1.39 (CP) y 3.60±1.55 (MC); hallándose también alta diversidad genética en ellos (heterocigosidades superiores al 75%), valores negativos en el FIS revelaron alto grado de introgresión; además el FST reveló estructura genética tanto en los grupos criollos (FST=0.02**), como en los departamentos muestreados (FST=0.039**). Según la distancia genética, los ovinos criollos colombianos presentan diferencias con los ovinos foráneos. Conclusiones. Los resultados obtenidos recomiendan proteger la ovinocultura criolla puesto que se encuentra amenazada por los constantes cruzamientos con razas foráneas, lo que conllevaría a pérdida de la identidad genética y de los rasgos de adaptación propios de los animales criollos.

ACS Style

Nini Vivas A; Vincenzo Landi; Jaime Muñoz Flórez; Moris Bustamante Yanez; Luz Álvarez Franco. Diversidad genética de ovinos criollos colombianos. Revista MVZ Córdoba 2020, 25, e2185 -e2185.

AMA Style

Nini Vivas A, Vincenzo Landi, Jaime Muñoz Flórez, Moris Bustamante Yanez, Luz Álvarez Franco. Diversidad genética de ovinos criollos colombianos. Revista MVZ Córdoba. 2020; 25 (3):e2185-e2185.

Chicago/Turabian Style

Nini Vivas A; Vincenzo Landi; Jaime Muñoz Flórez; Moris Bustamante Yanez; Luz Álvarez Franco. 2020. "Diversidad genética de ovinos criollos colombianos." Revista MVZ Córdoba 25, no. 3: e2185-e2185.

Journal article
Published: 08 August 2020 in Genetics Selection Evolution
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Background In this work, our aim was to generate a map of the copy number variations (CNV) segregating in a population of Murciano-Granadina goats, the most important dairy breed in Spain, and to ascertain the main biological functions of the genes that map to copy number variable regions. Results Using a dataset that comprised 1036 Murciano-Granadina goats genotyped with the Goat SNP50 BeadChip, we were able to detect 4617 and 7750 autosomal CNV with the PennCNV and QuantiSNP software, respectively. By applying the EnsembleCNV algorithm, these CNV were assembled into 1461 CNV regions (CNVR), of which 486 (33.3% of the total CNVR count) were consistently called by PennCNV and QuantiSNP and used in subsequent analyses. In this set of 486 CNVR, we identified 78 gain, 353 loss and 55 gain/loss events. The total length of all the CNVR (95.69 Mb) represented 3.9% of the goat autosomal genome (2466.19 Mb), whereas their size ranged from 2.0 kb to 11.1 Mb, with an average size of 196.89 kb. Functional annotation of the genes that overlapped with the CNVR revealed an enrichment of pathways related with olfactory transduction (fold-enrichment = 2.33, q-value = 1.61 × 10−10), ABC transporters (fold-enrichment = 5.27, q-value = 4.27 × 10−04) and bile secretion (fold-enrichment = 3.90, q-value = 5.70 × 10−03). Conclusions A previous study reported that the average number of CNVR per goat breed was ~ 20 (978 CNVR/50 breeds), which is much smaller than the number we found here (486 CNVR). We attribute this difference to the fact that the previous study included multiple caprine breeds that were represented by small to moderate numbers of individuals. Given the low frequencies of CNV (in our study, the average frequency of CNV is 1.44%), such a design would probably underestimate the levels of the diversity of CNV at the within-breed level. We also observed that functions related with sensory perception, metabolism and embryo development are overrepresented in the set of genes that overlapped with CNV, and that these loci often belong to large multigene families with tens, hundreds or thousands of paralogous members, a feature that could favor the occurrence of duplications or deletions by non-allelic homologous recombination.

ACS Style

Dailu Guan; Amparo Martínez; Anna Castelló; Vincenzo Landi; María Gracia Luigi-Sierra; Javier Fernández-Álvarez; Betlem Cabrera; Juan Vicente Delgado; Xavier Such; Jordi Jordana; Marcel Amills. A genome-wide analysis of copy number variation in Murciano-Granadina goats. Genetics Selection Evolution 2020, 52, 1 -10.

AMA Style

Dailu Guan, Amparo Martínez, Anna Castelló, Vincenzo Landi, María Gracia Luigi-Sierra, Javier Fernández-Álvarez, Betlem Cabrera, Juan Vicente Delgado, Xavier Such, Jordi Jordana, Marcel Amills. A genome-wide analysis of copy number variation in Murciano-Granadina goats. Genetics Selection Evolution. 2020; 52 (1):1-10.

Chicago/Turabian Style

Dailu Guan; Amparo Martínez; Anna Castelló; Vincenzo Landi; María Gracia Luigi-Sierra; Javier Fernández-Álvarez; Betlem Cabrera; Juan Vicente Delgado; Xavier Such; Jordi Jordana; Marcel Amills. 2020. "A genome-wide analysis of copy number variation in Murciano-Granadina goats." Genetics Selection Evolution 52, no. 1: 1-10.

Article
Published: 07 April 2020 in Italian Journal of Animal Science
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Domestic turkey production generally utilises only a few genetically improved lines, and local breeds are severely endangered as a result. Furthermore, the genetic resources of domestic turkeys have not been properly investigated, which could, ultimately, lead to the extinction of local breeds and negatively affect their corresponding genetic diversity and environmental adaptation. Although, several microsatellite markers have been designed for mapping and quantitative trait locus analysis, there is no standard panel of markers for genetic characterisation or genetic diversity assessment. Accordingly, the present study aimed to develop a set of polymorphic microsatellite markers that could be used for international turkey population studies. Thirty-nine microsatellites were selected based on polymorphism, DNA sequence and chromosome position, as well as on amplification efficiency, success rate and the absence of nonspecific amplification. The markers were screened using 105 DNA samples from local turkey breeds from Mexico, the United States, Italy, Brazil, Egypt and Spain. A total of 401 alleles were identified, with a mean number of alleles per marker of 10.28 ± 4.25. All microsatellites were polymorphic, with at least four alleles and no more than 19 alleles. Furthermore, allelic richness ranged from 3.810 to 17.985, mean heterozygosity ranged from 0.452 ± 0.229 to 0.667 ± 0.265, polymorphic information content values ranged from 0.213 (MNT264) to 0.850 (RHT0024) and the mean Fis value was 0.322. Overall, the panel was highly polymorphic and exhibited moderate Hardy–Weinberg disequilibrium, thereby indicating its value as a tool for biodiversity and population structure studies that could play an important role in promoting the conservation of local turkey breeds.

ACS Style

Amado Manuel Canales Vergara; Vincenzo Landi; Juan Vicente Delgado Bermejo; Amparo Martínez Martínez; Patricia Cervantes Acosta; Agueda Pons Barros; Daniele Bigi; Phillip Sponenberg; Mostafa Helal; Mohammad Hossein Banabazi; Maria Esperanza Camacho Vallejo. Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo). Italian Journal of Animal Science 2020, 19, 392 -398.

AMA Style

Amado Manuel Canales Vergara, Vincenzo Landi, Juan Vicente Delgado Bermejo, Amparo Martínez Martínez, Patricia Cervantes Acosta, Agueda Pons Barros, Daniele Bigi, Phillip Sponenberg, Mostafa Helal, Mohammad Hossein Banabazi, Maria Esperanza Camacho Vallejo. Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo). Italian Journal of Animal Science. 2020; 19 (1):392-398.

Chicago/Turabian Style

Amado Manuel Canales Vergara; Vincenzo Landi; Juan Vicente Delgado Bermejo; Amparo Martínez Martínez; Patricia Cervantes Acosta; Agueda Pons Barros; Daniele Bigi; Phillip Sponenberg; Mostafa Helal; Mohammad Hossein Banabazi; Maria Esperanza Camacho Vallejo. 2020. "Design and development of a multiplex microsatellite panel for the genetic characterisation and diversity assessment of domestic turkey (Meleagris gallopavo gallopavo)." Italian Journal of Animal Science 19, no. 1: 392-398.

Journal article
Published: 14 March 2020 in Genes
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Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits. Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components’ genetic parameters and breeding values. A total of 2594 lactation records were collected from 159 Murciano-Granadina goats (2005–2018), genotyped for 48 casein loci-located SNPs. Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits’ genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count). Milk yield, lactose, and somatic cell count heritabilities increased considerably when the model including genetic effects was considered (0.46, 0.30, 0.43, respectively). Components standard prediction errors decreased, and accuracies and reliabilities increased when genetic effects were considered. Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.

ACS Style

María Gabriela Pizarro Inostroza; Vincenzo Landi; Francisco Javier Navas González; Jose Manuel León Jurado; Juan Vicente Delgado Bermejo; Javier Fernández Álvarez; María Del Amparo Martínez Martínez. Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components. Genes 2020, 11, 309 .

AMA Style

María Gabriela Pizarro Inostroza, Vincenzo Landi, Francisco Javier Navas González, Jose Manuel León Jurado, Juan Vicente Delgado Bermejo, Javier Fernández Álvarez, María Del Amparo Martínez Martínez. Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components. Genes. 2020; 11 (3):309.

Chicago/Turabian Style

María Gabriela Pizarro Inostroza; Vincenzo Landi; Francisco Javier Navas González; Jose Manuel León Jurado; Juan Vicente Delgado Bermejo; Javier Fernández Álvarez; María Del Amparo Martínez Martínez. 2020. "Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components." Genes 11, no. 3: 309.

Journal article
Published: 22 December 2019 in Animals
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Despite Ecuador having a wide biodiversity of zootechnical species, characterization studies of these genetic resources are scarce. The objective of this research was to perform the biometric characterization of the Creole hen population through 14 quantitative zoometric measures. We evaluated 207 hens and 37 roosters from Ecuador’s three agro-ecological regions: the Sierra (highlands) region (Bolivar, Chimborazo, Tungurahua and Cotopaxi provinces); the Costa (coastal) region (Guayas); and the Oriente Amazonian region (Morona Santiago). Sexual dimorphism was assessed using one-way analysis of variance (ANOVA). Body dimensions were generally significantly higher for males (p < 0.05), especially for length of head, beak, neck, dorsum, tarsus, thigh, leg, and middle finger. Then, individuals were biometrically clustered into populations after a stepwise canonical discriminant analysis (CDA) computing interpopulation Mahalanobis distances. Agroecologically-based structured populations were identified when zoometrical criteria were used to classify the animals. Cotopaxi and Tungurahua provinces were reported to be the most distant from the rest, with a slight differentiation of the Morona Santiago province population from those in Guayas, Chimborazo and Bolívar. Conclusively, Ecuadorian Creole hens were higher than longer contrasting light hen breeds, which favors their dual-purpose aptitude. Hence, the development of selection programs aimed at Ecuadorian differentiated entity of production of eggs and meat across agro-ecological areas is feasible.

ACS Style

Paula Alexandra Toalombo Vargas; Francisco Javier Navas González; Vincenzo Landi; José Manuel León Jurado; Juan Vicente Delgado Bermejo. Sexual Dimorphism and Breed Characterization of Creole Hens through Biometric Canonical Discriminant Analysis across Ecuadorian Agroecological Areas. Animals 2019, 10, 32 .

AMA Style

Paula Alexandra Toalombo Vargas, Francisco Javier Navas González, Vincenzo Landi, José Manuel León Jurado, Juan Vicente Delgado Bermejo. Sexual Dimorphism and Breed Characterization of Creole Hens through Biometric Canonical Discriminant Analysis across Ecuadorian Agroecological Areas. Animals. 2019; 10 (1):32.

Chicago/Turabian Style

Paula Alexandra Toalombo Vargas; Francisco Javier Navas González; Vincenzo Landi; José Manuel León Jurado; Juan Vicente Delgado Bermejo. 2019. "Sexual Dimorphism and Breed Characterization of Creole Hens through Biometric Canonical Discriminant Analysis across Ecuadorian Agroecological Areas." Animals 10, no. 1: 32.

Journal article
Published: 28 November 2019 in Revista Mexicana de Ciencias Pecuarias
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Desde su llegada a México, las cabras han experimentado un largo proceso de adaptación y selección, dando como resultado animales locales de elevada rusticidad. Sin embargo, la importación de razas mejoradas y el intento de sustituir a los genotipos criollos por éstas, ha llevado a gran parte de este ganado al borde de la extinción. Un ejemplo es la cabra criolla negra (CCN), reconocida por su rusticidad y la calidad de su leche. El objetivo del presente trabajo fue caracterizar genéticamente una población de CCN. Se utilizaron 30 microsatélites, obteniendo; el número de alelos por marcador (NA), el número medio de alelos (MNA), número efectivo de alelos (Ae), la heterocigosidad observada (Ho) y esperada (He), el contenido de información polimórfica (PIC), el índice de fijación calculado con el estadístico FIS y el equilibrio de Hardy Weinberg (HWE). La población fue comparada con 13 razas caprinas del proyecto BioGoat. Los resultados mostraron que existe una elevada diversidad genética en este ganado. Se obtuvieron 243 alelos con un MNA de 8.1 alelos por locus. Los marcadores resultaron muy informativos con un PIC de 0.6. La He promedio de 0.71 y Ho de 0.62 puede indicar que existe un ligero desequilibrio en la población. La distancia de Reynolds mostró que la CCN se encuentra más distanciada genéticamente de la población Anglonubia y más cercana a la Murciano-Granadina. Los resultados aquí presentados sugieren que la población CCN representa una estructura racial bien diferenciada de las poblaciones incluidas en el estudio.

ACS Style

Juan Carlos Silva-Jarquin; Héctor Mario Andrade-Montemayor; Héctor Raymundo Vera-Ávila; Marina Durán-Aguilar; Sergio Iván Román-Ponce; Vincenzo Landi; Amparo Martínez-Martínez; Juan Vicente Delgado-Bermejo; Consorcio BioGoat. Diversidad y estructura genética de una población de cabras criollas negras de tres municipios del estado de Querétaro, México. Revista Mexicana de Ciencias Pecuarias 2019, 10, 801 -818.

AMA Style

Juan Carlos Silva-Jarquin, Héctor Mario Andrade-Montemayor, Héctor Raymundo Vera-Ávila, Marina Durán-Aguilar, Sergio Iván Román-Ponce, Vincenzo Landi, Amparo Martínez-Martínez, Juan Vicente Delgado-Bermejo, Consorcio BioGoat. Diversidad y estructura genética de una población de cabras criollas negras de tres municipios del estado de Querétaro, México. Revista Mexicana de Ciencias Pecuarias. 2019; 10 (4):801-818.

Chicago/Turabian Style

Juan Carlos Silva-Jarquin; Héctor Mario Andrade-Montemayor; Héctor Raymundo Vera-Ávila; Marina Durán-Aguilar; Sergio Iván Román-Ponce; Vincenzo Landi; Amparo Martínez-Martínez; Juan Vicente Delgado-Bermejo; Consorcio BioGoat. 2019. "Diversidad y estructura genética de una población de cabras criollas negras de tres municipios del estado de Querétaro, México." Revista Mexicana de Ciencias Pecuarias 10, no. 4: 801-818.

Original article
Published: 19 November 2019 in Journal of Animal Breeding and Genetics
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Goat milk casein proteins (αS1, αS2, β and κ) are encoded by four loci (CSN1S1, CSN1S2, CSN2 and CSN3, respectively) clustered within 250 kb in chromosome 6. In this study, 159 Murciano‐Granadina goats were genotyped for 48 SNPs within the entire casein region. Phenotypes on milk yield and components were obtained from 2,594 dairy registries. Additive and dominance effects on milk composition and quality were studied using non‐parametric tests and principal component analysis to prevent SNPs multicollinearity. Two deletions in exon 4 (CSN1S1 and CSN3), one in exon 7 (CSN2) and one in exon 15 (CSN1S2) have been found at frequencies ranging from 0.12 to 0.50. Bonferroni‐corrected significant SNP additive and dominance effects were found for milk yield, fat, protein, dry matter and lactose, and somatic cells. Exons 15 and 7 were significantly associated with milk yield and components except for lactose and somatic cells, while exon 4 was significantly associated with milk yield and components except for protein and dry matter. SNPs' associations with somatic cells were less frequent and weaker than those with milk yield and components. As caseins increase, somatic cells decrease, reducing milk enzymatic activity and consumption suitability. Hence, including molecular information in breeding schemes may promote production efficiency, as selecting against undesirable alleles could prevent the compromises derived from their dominance effects.

ACS Style

María Gabriela Pizarro Inostroza; Vincenzo Landi; Francisco Javier Navas González; José Manuel León Jurado; María Del Amparo Martínez Martínez; Javier Fernández Álvarez; Juan Vicente Delgado Bermejo. Non‐parametric association analysis of additive and dominance effects of casein complex SNPs on milk content and quality in Murciano‐Granadina goats. Journal of Animal Breeding and Genetics 2019, 137, 407 -422.

AMA Style

María Gabriela Pizarro Inostroza, Vincenzo Landi, Francisco Javier Navas González, José Manuel León Jurado, María Del Amparo Martínez Martínez, Javier Fernández Álvarez, Juan Vicente Delgado Bermejo. Non‐parametric association analysis of additive and dominance effects of casein complex SNPs on milk content and quality in Murciano‐Granadina goats. Journal of Animal Breeding and Genetics. 2019; 137 (4):407-422.

Chicago/Turabian Style

María Gabriela Pizarro Inostroza; Vincenzo Landi; Francisco Javier Navas González; José Manuel León Jurado; María Del Amparo Martínez Martínez; Javier Fernández Álvarez; Juan Vicente Delgado Bermejo. 2019. "Non‐parametric association analysis of additive and dominance effects of casein complex SNPs on milk content and quality in Murciano‐Granadina goats." Journal of Animal Breeding and Genetics 137, no. 4: 407-422.

Journal article
Published: 01 November 2019 in Animals
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According to recent archeological evidence, turkey (Meleagris gallopavo gallopavo) domestication may have occurred in Mexico around 2000 years ago. However, little is known about the phylogenetic and genealogical background underlying domestic turkey populations. This study aimed to further understand the domestication process and identify inter- or intraspecific connections between turkey populations to determine their origins, trace their global expansion, and define the species' genetic value. Ninety-three domestic turkeys (local breeds) were sampled from populations in Brazil, Mexico, USA, Spain, Italy, Iran, and Egypt. Publicly available sequences from previous studies were also included. Standard mitochondrial DNA, genetic diversity, and haplotype network analyses were performed. Seventy-six polymorphic sites were identified. Turkeys from Mexico showed the greatest number of polymorphic sites (40), while turkeys from Italy and Brazil reported only one site each. Nucleotide diversity was also highest in Mexico and the USA (π = 0.0175 and 0.0102, respectively) and lowest in Brazil and Italy. Of the six major haplogroups defined, the Mexican and USA populations appeared to have remained more stable and diverse than the other populations. This may be due to conservative husbandry policies in the rural areas of other populations, which have prevented the introduction of commercial turkey lines.

ACS Style

Amado Manuel Canales Vergara; Vincenzo Landi; Juan Vicente Delgado Bermejo; Amparo Martínez; Patricia Cervantes Acosta; Águeda Pons Barro; Daniele Bigi; Phillip Sponenberg; Mostafa Helal; Mohammad Hossein Banabazi; María Esperanza Camacho Vallejo. Tracing Worldwide Turkey Genetic Diversity Using D-loop Sequence Mitochondrial DNA Analysis. Animals 2019, 9, 897 .

AMA Style

Amado Manuel Canales Vergara, Vincenzo Landi, Juan Vicente Delgado Bermejo, Amparo Martínez, Patricia Cervantes Acosta, Águeda Pons Barro, Daniele Bigi, Phillip Sponenberg, Mostafa Helal, Mohammad Hossein Banabazi, María Esperanza Camacho Vallejo. Tracing Worldwide Turkey Genetic Diversity Using D-loop Sequence Mitochondrial DNA Analysis. Animals. 2019; 9 (11):897.

Chicago/Turabian Style

Amado Manuel Canales Vergara; Vincenzo Landi; Juan Vicente Delgado Bermejo; Amparo Martínez; Patricia Cervantes Acosta; Águeda Pons Barro; Daniele Bigi; Phillip Sponenberg; Mostafa Helal; Mohammad Hossein Banabazi; María Esperanza Camacho Vallejo. 2019. "Tracing Worldwide Turkey Genetic Diversity Using D-loop Sequence Mitochondrial DNA Analysis." Animals 9, no. 11: 897.

Journal article
Published: 15 October 2019 in Archivos de Zootecnia
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El pavo de traspatio mexicano es una raza autóctona en peligro de extinción, ya que en la actualidad se ha perdido la costumbre de conservar los recursos genéticos autóctonos de cada población, procurando el cruzamiento con animales de líneas comerciales para la producción de carne de pavo, dañando y perdiendo el acervo genético de los pavos autóctonos de México. El objetivo del presente trabajo es realizar la caracterización genética del pavo de traspatio mexicano mediante el uso de microsatélites y estudiar la posible estructura genética de esta población. Se analiza un panel de 38 microsatélites en 51 muestras de pavo de traspatio, tomadas de diferentes zonas de la ciudad de Veracruz, México. Se han evaluado los principales parámetros de diversidad genética: heterocigosidad esperada y observada, número de alelos, estadísticos F y Análisis Factorial de Correspondencia mediante el programa informático GENETIX. Se calculan las distancias genéticas entre individuos (DSA) con las que se ha construido un dendrograma utilizando el programa POPULATIONS. El árbol se visualiza con el programa TREEVIEW. Se estudia la estructura genética con el programa STRUCTURE. Todos los microsatélites utilizados han resultado polimórficos, encontrándose un mínimo de 2 alelos en el microsatélite MNT 264 y un máximo de 14 alelos en los marcadores MNT274 y RHT024, con un número medio de alelos de 6.79. Los valores medios de HE y HO son 0.619 y 0.620 respectivamente. Los estadísticos F muestran los siguientes valores en el total de la muestra: FIS 0.128 (P

ACS Style

A.M. Canales; Vincenzo Landi; A.M. Martínez; M. Macri; G. Pizarro; J. V. Delgado; P. Cervantes; A. Hernández; E. Camacho. Caracterización genética del pavo domestico de traspatio mexicano. Archivos de Zootecnia 2019, 68, 480 -487.

AMA Style

A.M. Canales, Vincenzo Landi, A.M. Martínez, M. Macri, G. Pizarro, J. V. Delgado, P. Cervantes, A. Hernández, E. Camacho. Caracterización genética del pavo domestico de traspatio mexicano. Archivos de Zootecnia. 2019; 68 (264):480-487.

Chicago/Turabian Style

A.M. Canales; Vincenzo Landi; A.M. Martínez; M. Macri; G. Pizarro; J. V. Delgado; P. Cervantes; A. Hernández; E. Camacho. 2019. "Caracterización genética del pavo domestico de traspatio mexicano." Archivos de Zootecnia 68, no. 264: 480-487.

Journal article
Published: 13 September 2019 in Animals
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A total of 2090 lactation records for 710 Murciano-Granadina goats were collected during the years 2005–2016 and analyzed to investigate the influence of the αS1-CN genotype on milk yield and components (protein, fat, and dry matter). Goats were genetically evaluated, including and excluding the αS1-CN genotype, in order to assess its repercussion on the efficiency of breeding models. Despite no significant differences being found for milk yield, fat and dry matter heritabilities, protein production heritability considerably increased after aS1-CN genotype was included in the breeding model (+0.23). Standard errors suggest that the consideration of genotype may improve the model’s efficiency, translating into more accurate genetic parameters and breeding values (PBV). Genetic correlations ranged from −0.15 to −0.01 between protein/dry matter and milk yield/protein and fat content, while phenotypic correlations were −0.02 for milk/protein and −0.01 for milk/fat or protein content. For males, the broadest range for reliability (RAP) (0.45–0.71) was similar to that of females (0.37–0.86) when the genotype was included. PBV ranges broadened while the maximum remained similar (0.61–0.77) for males and females (0.62–0.81) when the genotype was excluded, respectively. Including the αS1-CN genotype can increase production efficiency, milk profitability, milk yield, fat, protein and dry matter contents in Murciano-Granadina dairy breeding programs.

ACS Style

María Gabriela Pizarro Inostroza; Vincenzo Landi; Francisco Javier Navas González; Jose Manuel León Jurado; Amparo Martínez Martínez; Javier Fernández Álvarez; Juan Vicente Delgado Bermejo. Does the Acknowledgement of αS1-Casein Genotype Affect the Estimation of Genetic Parameters and Prediction of Breeding Values for Milk Yield and Composition Quality-Related Traits in Murciano-Granadina? Animals 2019, 9, 679 .

AMA Style

María Gabriela Pizarro Inostroza, Vincenzo Landi, Francisco Javier Navas González, Jose Manuel León Jurado, Amparo Martínez Martínez, Javier Fernández Álvarez, Juan Vicente Delgado Bermejo. Does the Acknowledgement of αS1-Casein Genotype Affect the Estimation of Genetic Parameters and Prediction of Breeding Values for Milk Yield and Composition Quality-Related Traits in Murciano-Granadina? Animals. 2019; 9 (9):679.

Chicago/Turabian Style

María Gabriela Pizarro Inostroza; Vincenzo Landi; Francisco Javier Navas González; Jose Manuel León Jurado; Amparo Martínez Martínez; Javier Fernández Álvarez; Juan Vicente Delgado Bermejo. 2019. "Does the Acknowledgement of αS1-Casein Genotype Affect the Estimation of Genetic Parameters and Prediction of Breeding Values for Milk Yield and Composition Quality-Related Traits in Murciano-Granadina?" Animals 9, no. 9: 679.

Journal article
Published: 11 September 2019 in Animals
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Latin American Creole chickens are generally not characterized; this is the case in Ecuador, where the lack of scientific information is contributing to their extinction. Here, we developed a characterization of the genetic resources of Ecuadorian chickens located in three continental agroecosystems (Pacific coastal, Andean, and Amazonian). Blood samples of 234 unrelated animals were collected in six provinces across Ecuador: Bolívar, Chimborazo, Cotopaxi, Guayas, Morona Santiago, and Tungurahua, in order to perform a genetic characterization and population structure assessment using the AVIANDIV project microsatellites panel (30 loci) and D-loop sequences of mitochondrial DNA and comparing with reference data from other breeds or genetic lines. The results indicate that Ecuadorian Creole chickens are the result of the admixture of different genetic groups that occurred during the last five centuries. While the influence of South Spanish breeds is demonstrated in the colonial age, genetic relationships with other breeds (Leghorn, Spanish fighter cock) cannot be discarded. The geographical configuration of the country and extreme climate variability have influenced the genetic isolation of groups constituting a homogeneous genetic status into the whole population. This is not only a source of genetic variation, but also a critical point because genetic drift produces a loss of genetic variants.

ACS Style

Paula Alexandra Toalombo Vargas; José Manuel León; Luis Rafael Fiallos Ortega; Amparo Martinez; Alex Arturo Villafuerte Gavilanes; Juan Vicente Delgado; Vincenzo Landi. Deciphering the Patterns of Genetic Admixture and Diversity in the Ecuadorian Creole Chicken. Animals 2019, 9, 670 .

AMA Style

Paula Alexandra Toalombo Vargas, José Manuel León, Luis Rafael Fiallos Ortega, Amparo Martinez, Alex Arturo Villafuerte Gavilanes, Juan Vicente Delgado, Vincenzo Landi. Deciphering the Patterns of Genetic Admixture and Diversity in the Ecuadorian Creole Chicken. Animals. 2019; 9 (9):670.

Chicago/Turabian Style

Paula Alexandra Toalombo Vargas; José Manuel León; Luis Rafael Fiallos Ortega; Amparo Martinez; Alex Arturo Villafuerte Gavilanes; Juan Vicente Delgado; Vincenzo Landi. 2019. "Deciphering the Patterns of Genetic Admixture and Diversity in the Ecuadorian Creole Chicken." Animals 9, no. 9: 670.